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Effects Of Herbs – Containing Phytoestrogens On Rat Testis: A Histological, Histochemical And Biochemical Study, Malak A. Al-Yawer

Iraqi Postgraduate Medical Journal

ABSTRACT: BACKGROUND: Estrogens has traditionally been known as the female hormone, but this idea has been challenged in early 1990’s and an essential physiological role for estrogen in male fertility was identified. Phytoestrogens are naturally occurring non-steroidal plant chemicals that can act like the female hormone estrogen. The herbs ( anise alfalfa and vervain ) chosen in this study contain phytoestrogens. OBJECTIVE: Previous studies demonstrated controversy of the effects of phytoestrogens on the rat testes .Hence, the present investigation was undertaken to investigate the influence of typical dose of herbs containing phytoestrogen on the rat testis. MATERIALS AND METHODS: Twenty-four …

Synthesis And Characterization Of Amphiphilic Cyclic Diblock Copolypeptoids From N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization Of N-Substituted N-Carboxyanhydride, Chang Uk Lee, Thomas P. Smart, Li Guo, Thomas H. Epps, Donghui Zhang

Faculty Publications

N-Heterocyclic carbene (NHC)-mediated ring-opening polymerization of N-decyl-N-carboxylanhydride monomer (De-NCA) has been shown to occur in a controlled manner, yielding cyclic poly(N-decylglycine)s (c-PNDGs) with polymer molecular weights (MW) between 4.8 and 31 kg mol -1 and narrow molecular weight distributions (PDI < 1.15). The reaction exhibits pseudo-first-order kinetics with respect to monomer concentration. The polymer MW increases linearly with conversion, consistent with a living polymerization. ESI MS and SEC analyses confirm the cyclic architectures of the forming polymers. DSC and WAXS studies reveal that the c-PNDG homopolymers are highly crystalline with two prominent first-order transitions at 72-79 °C (T m,1) and 166-177 °C (T m,2), which have been attributed to side chain and main chain melting, respectively. A series of amphiphilic cyclic diblock copolypeptoids [i.e., poly(N-methylglycine)-b-poly(N- decylglycine) (c-PNMG-b-PNDG)] with variable molecular weight and composition were synthesized by sequential NHC-mediated polymerization of the corresponding N-methyl-N-carboxyanhydride (Me-NCA) and De-NCA monomers. 1H NMR analysis reveals that adjusting the initial monomer to NHC molar ratio can readily control the block copolymer chain length and composition. Time-lapsed light scattering and cryogenic transmission electron microscopy (cryo-TEM) analyses of c-PNDG-b-PNMG samples showed that the amphiphilic cyclic block copolypeptoids self-assemble into spherical micelles that reorganize into micrometer-long cylindrical micelles with uniform diameter in room temperature methanol over the course of several days. An identical morphological transition has also been noted for the linear analogues, which occurs more rapidly than for the cyclic copolypeptoids. We tentatively attribute this difference to the different crystallization kinetics of the solvophobic block (i.e., PNDG) in the cyclic and linear block copolypeptoids. © 2011 American Chemical Society.

Cd2ap Regulates Sumoylation Of Cin85 In Podocytes, Irini Tossidou, Rainer Niedenthal, Malte Klaus, Beina Teng, Kirstin Worthmann, Benjamin King, Kevin Peterson

Dartmouth Scholarship

Podocytes are highly differentiated and polarized epithelial cells located on the visceral side of the glomerulus. They form an indispensable component of the glomerular filter, the slit diaphragm, formed by several transmembrane proteins and adaptor molecules. Disruption of the slit diaphragm can lead to massive proteinuria and nephrotic syndrome in mice and humans. CD2AP is an adaptor protein that is important for the maintenance of the slit diaphragm. Together with its paralogue, CIN85, CD2AP belongs to a family of adaptor proteins that are primarily described as being involved in endocytosis and downregulation of receptor tyrosine kinase activity. We have shown …

Natural Variation In Gestational Cortisol Is Associated With Patterns Of Growth In Marmoset Monkeys (Callithrix Geoffroyi), Aaryn C. Mustoe, Andrew K. Birnie, Andrew V. Korgan, Jonathan Bruce Santo, Jeffrey French

Psychology Faculty Publications

High levels of prenatal cortisol have been previously reported to retard fetal growth. Although cortisol plays a pivotal role in prenatal maturation, heightened exposure to cortisol can result in lower body weights at birth, which have been shown to be associated with adult diseases like hypertension and cardiovascular disease. This study examines the relationship between natural variation in gestational cortisol and fetal and postnatal growth in marmoset monkeys. Urinary samples obtained during the mother’s gestation were analyzed for cortisol. Marmoset body mass index (BMI) was measured from birth through 540 days in 30- or 60-day intervals. Multi-level modeling was used …

Cluster Control Of Automated Surface Vessels, Paul David Mahacek

Mechanical Engineering Senior Theses

This research focuses on the design and control of a fleet of robotic kayaks, and presents experimental data regarding the functionality and performance of the system. One of the key technical challenges in fielding multi-robot systems for real-world applications is the coordination and relative motion control of the individual units. Coordinated formation control of the fleet is implemented through the use of the cluster space control architecture, which is a full-order controller that treats the fleet as a virtual, articulating, kinematic mechanism. The resulting system is capable of autonomous navigation utilizing a centralized controller, currently implemented via a shore-based computer …

Science With A Skew: The Nuclear Power Industry After Chernobyl And Fukushima, Gayle Greene

Scripps Faculty Publications and Research

It is one of the marvels of our time that the nuclear industry managed to resurrect itself from its ruins at the end of the last century, when it crumbled under its costs, inefficiencies, and mega-accidents. Chernobyl released hundreds of times the radioactivity of the Hiroshima and Nagasaki bombs combined, contaminating more than 40% of Europe and the entire Northern Hemisphere. But along came the nuclear lobby to breathe new life into the industry, passing off as “clean” this energy source that polluted half the globe. The “fresh look at nuclear”—in the words of a New York Times makeover piece …

Deafdigest: Gold Edition, December 25, 2011, Barry Strassler

DeafDigest Gold 2011

No abstract provided.

Defisiensi Riboflavin Dan Demensia Pada Usia Lanjut, Idral Purnakarya

Kesmas

Demensia Alzheimer menempati urutan kesembilan penyebab kematian di Amerika Serikat. Demensia adalah kondisi yang sering dialami yang berhubungan dengan berbagai faktor dan gaya hidup terutama diet. Penelitian ini bertujuan untuk mengetahui hubungan defisiensi asupan riboflavin (vitamin B12) dengan demensia pada usia lanjut (usila). Penelitian ini menggunakan desain studi cross sectional dan dilaksanakan pada bulan September 2007 sampai dengan Januari 2008. Sampel penelitian adalah 141 lansia berumur lebih dari sama dengan 60 tahun yang diambil secara purposive sampling. Demensia diukur menggunakan kuesioner MMSE (² 24, skor maksimum 30) dan asupan riboflavin diukur menggunakan form Semi Quantitative – FFQ. Penelitian ini memperlihatkan …

Biosensors For Brain Trauma And Dual Laser Doppler Flowmetry: Enoxaparin Simultaneously Reduces Stroke-Induced Dopamine And Blood Flow While Enhancing Serotonin And Blood Flow In Motor Neurons Of Brain, In Vivo, Patricia A. Broderick, Edwin H. Kolodny

Publications and Research

Neuromolecular Imaging (NMI) based on adsorptive electrochemistry, combined with Dual Laser Doppler Flowmetry (LDF) is presented herein to investigate the brain neurochemistry affected by enoxaparin (Lovenox®), an antiplatelet/antithrombotic medication for stroke victims. NMI with miniature biosensors enables neurotransmitter and neuropeptide (NT) imaging; each NT is imaged with a response time in milliseconds. A semiderivative electronic reduction circuit images several NT’s selectively and separately within a response time of minutes. Spatial resolution of NMI biosensors is in the range of nanomicrons and electrochemically-induced current ranges are in pico- and nano-amperes. Simultaneously with NMI, the LDF technology presented herein operates on line …

Learning The Structure Of Gene Regulatory Networks From Time Series Gene Expression Data, Haoni Li, Nan Wang, Ping Gong, Edward J. Perkins, Chaoyang Zhang

Faculty Publications

Background: Dynamic Bayesian Network (DBN) is an approach widely used for reconstruction of gene regulatory networks from time-series microarray data. Its performance in network reconstruction depends on a structure learning algorithm. REVEAL (REVerse Engineering ALgorithm) is one of the algorithms implemented for learning DBN structure and used to reconstruct gene regulatory networks (GRN). However, the two-stage temporal Bayes network (2TBN) structure of DBN that specifies correlation between time slices cannot be obtained by score metrics used in REVEAL.

Methods: In this paper, we study a more sophisticated score function for DBN first proposed by Nir Friedman for stationary …

Transcriptomic Profiles Of Peripheral White Blood Cells In Type Ii Diabetes And Racial Differences In Expression Profiles, Jinghe Mao, Junmei Ai, Xinchun Zhou, Ming Shenwu, Manuel Ong Jr., Marketta Blue, Jasmine T. Washington, Xiaonan Wang, Youping Deng

Faculty Publications

Background: Along with obesity, physical inactivity, and family history of metabolic disorders, African American ethnicity is a risk factor for type 2 diabetes (T2D) in the United States. However, little is known about the differences in gene expression and transcriptomic profiles of blood in T2D between African Americans (AA) and Caucasians (CAU), and microarray analysis of peripheral white blood cells (WBCs) from these two ethnic groups will facilitate our understanding of the underlying molecular mechanism in T2D and identify genetic biomarkers responsible for the disparities.

Results: A whole human genome oligomicroarray of peripheral WBCs was performed on 144 …

Structural Analysis Of Proinsulin Hexamer Assembly By Hydroxyl Radical Footprinting And Computational Modeling, Janna G. Kiselar, Manish Datt, Mark R. Chance, Michael A. Weiss

Faculty Scholarship

Mutations in the insulin gene can impair proinsulin folding and cause diabetes mellitus. Although crystal structures of insulin dimers and hexamers are well established, proinsulin is refractory to crystallization. Although an NMR structure of an engineered proinsulin monomer has been reported, structures of the wild-type monomer and hexamer remain undetermined. We have utilized hydroxyl radical footprinting and molecular modeling to characterize these structures. Differences between the footprints of insulin and proinsulin, defining a "shadow" of the connecting (C) domain, were employed to refine the model. Our results demonstrate that in its monomeric form, (i) proinsulin contains a native-like insulin moiety …

Talented Students And Resilient At-Risk Students: Similarities And Differences, Frank C. Worrell

Gifted Children

This study compared academically talented students (n = 23) who were not at risk for school failure and resilient at-risk students (n = 27) on risk factors and protective/promotive factors. Participants’ risk status was determined by student assignment. The academically talented students were attending a summer program at a major research university and the at-risk students were graduates of a continuation high school for students who had had numerous infractions at regular high schools. As expected, the two groups differed on risk factors and on factors related to academic status. However, the groups did not differ on psychosocial variables related …

Cal Poly Professor, Colleagues Discover New Magnetic Bacterium, Public Affairs Office

Press Releases

No abstract provided.

Pentachlorophenol Decreases Tumor-Cell-Binding Capacity And Cell-Surface Protein Expression Of Human Natural Killer Cells, Tasia Hurd, Jasmine Walker, Margaret M. Whalen

Chemistry Faculty Research

Pentachlorophenol (PCP) is an organochlorine pesticide that decreases the tumor-cell killing (lytic) function of human natural killer (NK) cells. NK cells defend against tumor cells and virally infected cells. They bind to these targets, utilizing a variety of cell-surface proteins. This study examined concentrations of PCP that decrease lytic function for alteration of NK binding to tumor targets. Levels of PCP that caused loss of binding function were then examined for effects on expression of cell-surface proteins needed for binding. Exposure to 10 µm PCP for 24 h (which caused a greater than 70% loss of lytic function) decreased NK …

Differential Effects Of Pravastatin And Simvastatin On The Growth Of Tumor Cells From Different Organ Sites, David G. Menter, Victoria P. Ramsauer, Sam Harirforoosh, Kanishka Chakraborty, Peiying Yang, Linda His, Robert A. Newman, Koyamangalath Krishnan

Pharmacy Faculty Articles and Research

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) inhibitors, commonly known as statins, may possess cancer preventive and therapeutic properties. Statins are effective suppressors of cholesterol synthesis with a well-established risk-benefit ratio in cardiovascular disease prevention. Mechanistically, targeting HMGCR activity primarily influences cholesterol biosynthesis and prenylation of signaling proteins. Pravastatin is a hydrophilic statin that is selectively taken up by a sodium-independent organic anion transporter protein-1B1 (OATP1B1) exclusively expressed in liver. Simvastatin is a hydrophobic statin that enters cells by other mechanisms. Poorly-differentiated and well-differentiated cancer cell lines were selected from various tissues and examined for their response to these two statins. Simvastatin …

A Method For Measuring Programmatic Dependency And Interdependency Between Dod Acquisition Programs, Matthew B. Christensen

Theses and Dissertations

There is currently no accepted method for quantitative measurement of acquisition program interdependency. While some methods exist for measuring dependency or interdependency at the component or system level, these methods do not translate well to program interdependency measurement. The objective of this thesis is to provide a model for measuring acquisition program interdependency accurately and quantitatively. The model presented in this thesis uses four Interdependency Factors to identify dependency relationships between programs. Specific Interdependency Levels are then used to measure the strengths of those dependencies. The model also accounts for measurement of dependencies upon programs that are not directly connected, …

Electroluminescence Studies On Longwavelength Indium Arsenide Quantum Dot Microcavities Grown On Gallium Arsenide, John C. Ramsey

Theses and Dissertations

A comprehensive study of the electroluminescence of four GaAs/AlGaAs microcavity devices with InAs/GaInAs quantum dot active regions emitting near 1.3 µm was conducted. The four molecular beam epitaxial grown samples with AlAs oxide aperture confinement layers were fabricated, characterized, and optically modeled. Optical power transmission of the samples was modeled using Matlab and compared with measured transmission data. Resonant cavity light emitting diodes (RCLEDs) and three vertical cavity surface emitting laser (VCSEL) samples were fabricated and electro-optically characterized over a range of injection currents and temperatures. Devices achieved continuous wave room temperature lasing at 1.28 µm with an output power …

Student Center, Science Wing Set To Open In 2012, Office Of University Communications

Gardner-Webb NewsCenter Archive

Rarely has Gardner-Webb looked forward to the New Year with more excitement than this winter, as the University anticipates opening, in the fall of 2012, the long-awaited Tucker Student Center and the new science research wing. The buildings represent the culmination of the University’s “Higher Ground Campaign,” and they manifest the generous and continued support of the University’s most faithful friends and donors. As GWU President Dr. Frank Bonner says, when it opens, the Tucker Student Center will be “the single-most impressive and most transformational building ever built on Gardner-Webb’s campus.”

A Shared Gene Expression Signature In Mouse Models Of Ebv-Associated And Non-Ebv-Associated Burkitt Lymphoma, Kathryn T. Bieging, Kamonwan Fish, Subbarao Bondada, Richard Longnecker

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The link between EBV infection and Burkitt lymphoma (BL) is strong, but the mechanism underlying that link has been elusive. We have developed a mouse model for EBV-associated BL in which LMP2A, an EBV latency protein, and MYC are expressed in B cells. Our model has demonstrated the ability of LMP2A to accelerate tumor onset, increase spleen size, and bypass p53 inactivation. Here we describe the results of total gene expression analysis of tumor and pretumor B cells from our transgenic mouse model. Although we see many phenotypic differences and changes in gene expression in pretumor B cells, the transcriptional …

Development Of An Environmentally Friendly Binder System For Paper Products, Ashley Rhea Flory

Student Theses and Dissertations

It is important for industries to find ways to find green chemistries to protect the environment and the paper industry is no exception. Using renewable resources such as plant material will be an excellent substitute for the chemicals that are currently used as paper binders. In this project, carbohydrates, proteins and phenolic compounds were applied to air laid, pressed paper in order to obtain a binder that is as strong as the currently used factory binders while being organic and renewable. Out of the twenty binders surveyed soy protein, gelatin, zein protein, pectin and Salix lignin provided comparable strength results …

Mechanism Of Acetylcholinesterase's Neurotrophic Action On Drg Neurons Of Rat, Naga Venkata Pavan Kumar Kunala

Student Theses and Dissertations

The fundamental role of enzyme acetylcholinesterase (AChE) is to catalyze the neurotransmitter acetylcholine at cholinergic synapses. In addition to its catalytic activity, AChE appears to play a significant non-catalytic role of neurotropic activity on dorsal root ganglion (DRG) neurons of rat. However the mechanism responsible for AChE's neurotrophic activity is not fully understood. Earlier experiments in Dr. Srivatsan's laboratory demonstrated the binding of fluorophore conjugated AChE to cell surface of DRG neurons. These findings led to my hypothesis that AChE may bind to cell surface protein(s) in DRG neurons, and the resulting protein-protein interaction is responsible for activating downstream signaling …

Efficacy Of A Novel Chelator For Mercury Chelation And Distribution, David Harold Clarke

Student Theses and Dissertations

Mercury is found throughout the environment with a distribution on a global scale in nearly all terrestrial to marine environments. Biotransformation of divalent mercury and subsequent bioaccumulation of organo-mercury species is catalyzed by sulfate reducing bacteria. Trophic transport of the resultant methyl mercury, a highly toxic species, leads to upward accumulation in the trophic structure of aquatic environments and ultimately to human exposure through consumption of methyl mercury laden fish. The potential for human health risk has made mercury a high priority environmental contaminant. Research aimed at assessing the within-organism fate of mercury using a genetically tractable mammalian model is …

Characteristics Of Extreme Droughts Inferred From Tree-Ring Data In The Qilian Mountains, 1700−2005, Yong Zhang, Xuemei Shao, Zhi-Yong Yin, Eryuan Liang, Qinhua Tian, Yan Xu

Environmental and Ocean Sciences: Faculty Scholarship

Severe droughts were evaluated using a tree-ring network of 12 chronologies (1700−2005) from the Qilian Mountains on the northeastern Tibetan Plateau. A total of 98 drought years, including 78 moderate, 15 severe, and 5 extreme events, were identified for the past 306 yr. Following the starting year of a drought phase, drought commonly continued for 1 to 2 yr. The spatial patterns of moisture conditions in central and eastern China corresponding to 19 major drought years during the period 1700−2000 were grouped into 3 clusters, with one particular pattern showing droughts of nation-wide impact (i.e. 1721, 1928 and 1966). Drought …

Differential Effects Of Pravastatin And Simvastatin On The Growth Of Tumor Cells From Different Organ Sites, David G. Menter, Victoria P. Ramsauer, Sam Harirforoosh, Kanishka Chakraborty, Peiying Yang, Linda Hsi, Robert A. Newman, Koyamangalath Krishnan

ETSU Faculty Works

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) inhibitors, commonly known as statins, may possess cancer preventive and therapeutic properties. Statins are effective suppressors of cholesterol synthesis with a well-established risk-benefit ratio in cardiovascular disease prevention. Mechanistically, targeting HMGCR activity primarily influences cholesterol biosynthesis and prenylation of signaling proteins. Pravastatin is a hydrophilic statin that is selectively taken up by a sodium-independent organic anion transporter protein-1B1 (OATP1B1) exclusively expressed in liver. Simvastatin is a hydrophobic statin that enters cells by other mechanisms. Poorly-differentiated and well-differentiated cancer cell lines were selected from various tissues and examined for their response to these two statins. Simvastatin …

Functional Analysis Of The Kinome Of The Wheat Scab Fungus Fusarium Graminearum., Chenfang Wang, Shijie Zhang, Rui Hou, Zhongtao Zhao, Qian Zheng, Qijun Xu, Dawei Zheng, Guanghui Wang, Huiquan Liu, Xuli Gao, Ji-Wen Ma, H Corby Kistler, Zhensheng Kang, Jin-Rong Xu

Department of Botany and Plant Pathology Faculty Publications

As in other eukaryotes, protein kinases play major regulatory roles in filamentous fungi. Although the genomes of many plant pathogenic fungi have been sequenced, systematic characterization of their kinomes has not been reported. The wheat scab fungus Fusarium graminearum has 116 protein kinases (PK) genes. Although twenty of them appeared to be essential, we generated deletion mutants for the other 96 PK genes, including 12 orthologs of essential genes in yeast. All of the PK mutants were assayed for changes in 17 phenotypes, including growth, conidiation, pathogenesis, stress responses, and sexual reproduction. Overall, deletion of 64 PK genes resulted in …

Morpholino Gene Knockdown In Adult Fundulus Heteroclitus: Role Of Sgk1 In Seawater Acclimation, Emily G. Notch, Joseph R. Shaw, Bonita A. Coutermarsh, Marisa Dzioba, Bruce A. Stanton

Dartmouth Scholarship

The Atlantic killifish (Fundulus heteroclitus) is an environmental sentinel organism used extensively for studies on environmental toxicants and salt (NaCl) homeostasis. Previous research in our laboratory has shown that rapid acclimation of killifish to seawater is mediated by trafficking of CFTR chloride channels from intracellular vesicles to the plasma membrane in the opercular membrane within the first hour in seawater, which enhances chloride secretion into seawater, thereby contributing to salt homeostasis. Acute transition to seawater is also marked by an increase in both mRNA and protein levels of serum glucocorticoid kinase 1 (SGK1) within 15 minutes of transfer. …

High Connectivity In The Deepwater Snapper Pristipomoides Filamentosus (Lutjanidae) Across The Indo-Pacific With Isolation Of The Hawaiian Archipelago, Michelle R. Gaither, Shelley A. Jones, Christopher Kelley, Stephen J. Newman, Laurie Sorenson, Brian W. Bowen

Fisheries Research Articles

In the tropical Indo-Pacific, most phylogeographic studies have focused on the shallow-water taxa that inhabit reefs to approximately 30 m depth. Little is known about the large predatory fishes, primarily snappers (subfamily Etelinae) and groupers (subfamily Epinephelinae) that occur at 100–400 m. These long-lived, slow-growing species support fisheries across the Indo-Pacific, yet no comprehensive genetic surveys within this group have been conducted. Here we contribute the first range-wide survey of a deepwater Indo-Pacific snapper, Pristipomoides filamentosus, with special focus on Hawai'i. We applied mtDNA cytochrome b and 11 microsatellite loci to 26 samples (N = 1,222) collected across …

Cns Recruitment Of Cd8+ T Lymphocytes Specific For A Peripheral Virus Infection Triggers Neuropathogenesis During Polymicrobial Challenge., Christine M Matullo, Kevin J O'Regan, Mark Curtis, Glenn F Rall

Department of Microbiology and Immunology Faculty Papers

Although viruses have been implicated in central nervous system (CNS) diseases of unknown etiology, including multiple sclerosis and amyotrophic lateral sclerosis, the reproducible identification of viral triggers in such diseases has been largely unsuccessful. Here, we explore the hypothesis that viruses need not replicate in the tissue in which they cause disease; specifically, that a peripheral infection might trigger CNS pathology. To test this idea, we utilized a transgenic mouse model in which we found that immune cells responding to a peripheral infection are recruited to the CNS, where they trigger neurological damage. In this model, mice are infected with …

Comparison Of Impairments, Activity Limitations, Physical Activity, And Self-Efficacy Among Healthy Weight, Overweight And Obese Minority Middle School Children, Annabel Nunez-Gaunaurd

Open Access Dissertations

The feasibility and outcomes of a 12-week extracurricular family-based intervention led by physical therapists that was designed to increase physical activity (PA) in three Hispanic male middle-school children was examined. This intervention has limited feasibility and may increase physical activity levels for overweight Hispanic middle school children. In a second study, differences in motor proficiency, strength, endurance, and PA among healthy weight, overweight and obese children were examined, and correlations between BMI and physical impairments were explored. Obese children demonstrated impairments in motor proficiency, strength, and endurance when compared to healthy weight children. Among overweight children, higher BMI was associated …