Digital Commons Network^™

Mutation Of The Hof1 Pest Domain Affects Cytokinesis In Budding Yeast, Katherine E. Stockstill

Masters Theses

"Cytokinesis is the division of a cell into two daughter cells. Cytokinesis failure results in polyploidy, which may contribute to tumorigenesis or cause cell death. The Saccharomyces cerevisiae protein Hof1, which is required for efficient cytokinesis, is phosphorylated during mitosis. The hypothesis that MEN (mitotic exit network) dependent phosphorylation regulates Hof1 degradation, triggering completion of cytokinesis was tested. Hof1 contains a PEST sequence, which is rich in proline, glutamic acid, serine, and threonine, and is believed to be involved in protein degradation. To test the hypothesis, we created two non-phosphorylatable mutants of the Hof1 PEST domain tagged with GFP. The …

Production And Characterization Of Supported Palladium Nanoparticles On Multiwalled Carbon Nanotubes By Gamma Irradiation, Jessika Viviana Rojas Marin

Masters Theses

"Carbon nanotubes are being studied for a variety of applications due to their outstanding mechanical, chemical, electrical, and optical properties that make them interesting in different areas. Nowadays, different methods to modify the structure of the nanotubes are being developed in order to expand the application fields of such materials. In this work, palladium nanoparticles were directly produced and supported on multi-walled carbon nanotubes (MWCNT) by gamma irradiation. A solution with a 2:1 water-isopropanol ratio was prepared and mixed with palladium chloride as precursor of palladium ions. Radiolysis of water produces certain species that reduce the ions down to a …

Integration Of Model-Based Systems Engineering And Virtual Engineering Tools For Detailed Design, Akshay Kande

Masters Theses

"Design and development of a system can be viewed as a process of transferring and transforming data using a set of tools that form the system's development environment. Conversion of the systems engineering data into useful information is one of the prime objectives of the tools used in the process. With complex systems, the objective is further augmented with a need to represent the information in an accessible and comprehensible manner. The importance of representation is easily understood in light of the fact that the stakeholder's ability to make prompt and appropriate decisions is directly related to his understanding of …

Assess Commercialization Feasibility Of Algae-Based Bio-Fuel With Dashboard And Scorecard Technologies, Madhur Chopra

Masters Theses

"The growing energy depleting energy supplies and increasing environmental pollution has motivated researchers across the globe to look for alternative energy sources. Various energy sources have been identified but none of them have the capability of realistically replacing the fossil fuels and meeting the current demand. Algae based bio-fuels give some hope but producing them at large scale is a challenge which needs to be overcome.

The underlying objective of this research project is two folds. Firstly, the project aims to assist with the commercialization of algae based bio-fuels by developing Dashboard based and Balanced Scorecard based monitoring prototypes. The …

Improving Student Learning Outcomes Through Personal Response Systems (Clickers), Ran Cai

Masters Theses

"The main purpose of this research was to examine the impact of Personal Response Systems (PRS) or clickers, (as they are commonly called), on student learning outcome. Student learning outcomes as measured by course grades were compared between traditional lecture format and clicker use lecture format. Student perceptions on clicker use were observed in the mean time. Statistical analysis indicated that although student conveyed positive attitude on clicker adoption, there were no significant differences in course scores between students who used clickers and students who did not.

This study also considered the influence of other factors, such as gender, student …

Design, Analysis, And Optimization Of Composite Structures For Aerospace Applications, Tyler Jason Keil

Masters Theses

"This thesis contains three papers that focus on composites that pertain to a variety of structures commonly found in the aerospace industry. The first paper presents an investigation of the stowage and deployment strength of a thin carbon fiber-reinforced polymer shell capable of being rolled and stowed in a tight rolled configuration. The intent of this structure is for use as a segment of a larger deployable reflective aperture. This study involved finite element analysis of several laminates, coupon-level and full-scale prototype testing, which provided a much needed insight and benchmark of composite shells used in this manner.

The second …

Veille Bibliographique Sur La Surveillance Biologique De L'Exposition Aux Produits Chimiques En Milieu De Travail, Annie Bijaoui, Florence Pillière, Maryse Gagnon, Ginette Truchon, Michèle Berode, Aline Detorrenté, Aline Sager, Perrine Hoët

Vulgarisation

L’article présente les étapes de la mise en place d’une veille bibliographique (ou veille scientifique) thématique effectuée conjointement depuis 2005 par 4 institutions francophones du domaine de la santé au travail : l’INRS (France), l’IRSST (Québec), l’IST (Suisse) et l’UCL (Belgique). La thématique suivie est celle de la surveillance biologique de l’exposition aux produits chimiques en milieu de travail. Les données recueillies et mises en forme par les documentalistes servent aux chercheurs spécialistes du sujet non seulement pour suivre les nouveautés du domaine, mais aussi pour documenter des cours et mettre à jour des guides de surveillance biologique. Les différentes …

Risk Factors For Running Away Among A General Population Sample Of Males And Females, Kimberly A. Tyler, Kellie J. Hagewen, Lisa A. Melander

Department of Sociology: Faculty Publications

The present study examines risk factors for running away and homelessness among a sample of more than 7,000 currently housed youth using the National Longitudinal Study of Adolescent Health (Add Health). Structural equation modeling results revealed that those with greater levels of family instability and those who ran away at Wave 2 were significantly more likely to run away and/or become homeless 5 years later at Wave 3. Family instability also had a significant indirect effect on running away and/or being homeless at Wave 3 through greater levels of problem behaviors and running away at Wave 2. Running away at …

Pregnancy And Intimate Partner Violence: Risk Factors, Severity, And Health Effects, Douglas A. Brownridge, Tamara L. Tallieu, Kimberly A. Tyler, Agnes Tiwari, Ko Ling Chan, Susy C. Santos

Department of Sociology: Faculty Publications

The current study compares female victims of intimate partner violence (IPV) who were and were not victimized during pregnancy. Victims of pregnancy violence are more likely to report having experienced all forms of violence, particularly severe forms, and have higher odds of experiencing several postviolence indicators of severity and adverse health consequences. The significance of predictors disappears in a post hoc analysis controlling for proxies of battering behavior (i.e., repeated and severe violence), suggesting that victims who experience violence during pregnancy may be more likely to be in a current intimate relationship with an abuser who inflicts repeated and severe …

Effect Of School Racial Composition On Trajectories Of Depressive Symptoms From Adolescence Through Early Adulthood, Katrina M. Walsemann, Bethany A. Bell, Bridget J. Goosby

Department of Sociology: Faculty Publications

We investigate the effect of high school racial composition, measured as percent of non-Hispanic white students, on trajectories of depressive symptoms from adolescence to early adulthood. We also explore whether the effect of school racial composition varies by respondent race/ethnicity and whether adult socioeconomic status mediates this relationship. We analyzed four waves of data from the National Longitudinal Study of Adolescent Health using 3-level linear growth models. We restricted our sample to respondents enrolled in grades 9-12 in 1994/5 who were interviewed at a minimum in Waves I and IV. This resulted in 10,350 respondents enrolled in 80 high schools …

Variation In Distress Among Women With Infertility: Evidence From A Population-Based Sample, Arthur L. Greil, Karina M. Shreffler, Lone Schmidt, Julia Mcquillan

Department of Sociology: Faculty Publications

BACKGROUND We examine variation in fertility-specific distress (FSD) and general distress according to different experiences of infertility among 1027 US women who have experienced infertility within the previous 10 years.

METHODS General distress was measured by a short form of the Center for Epidemiological Studies-Depression. Multiple regression analysis was conducted on self-report data (based on a telephone interview) from a probability-based sample of US women aged 25–45 years. We compare women with infertility who have had a prior pregnancy (secondary infertility, n = 628) to women with infertility with no prior pregnancies (primary infertility, n = 399). We further distinguish …

Are Prior Pregnancy Outcomes Relevant For Models Of Fertility-Specific Distress Or Infertility Helpseeking?, Arthur L. Greil, Katherine M. Johnson, Julia Mcquillan, Naomi L Lacy

Department of Sociology: Faculty Publications

Women with prior pregnancy but no live birth are inconsistently termed as either ‘primary infertile’ or ‘secondary infertile’ in psychosocial studies of infertile women. The goal of this study was to discover whether infertile women who had experienced pregnancies but no live births were more similar in attitudes and behavior to infertile women who had not experienced pregnancies or to those who had live births. We used the National Survey of Fertility Barriers (NSFB), which contains self-reported data from a probability-based sample of US women aged between 25 and 45, to accomplish our goal. In this cross-sectional analysis, infertile women …

Nicotine Competes With A Visual Stimulus For Control Of Conditioned Responding, Jennifer E. Murray, Nicole R. Wells, Rick A. Bevins

Department of Psychology: Faculty Publications

Environmental stimuli that co-occur with tobacco use come to evoke drug-related conditioned responses (CRs) that appear involved in continued use of nicotine-containing products. In rats, nicotine can serve as a conditional stimulus (CS) for non-drug unconditioned stimuli (USs), prompting the question of whether the nicotine CS can compete with, or overshadow, a non-drug environmental stimulus for control of a CR. In Experiment 1, male Sprague-Dawley rats were assigned to a group [0, 0.01, 0.03, 0.045, or 0.06 mg nicotine (base)/kg/infusion]. During each session, there were 10 intravenous infusions followed by a 30-second houselight to form a compound CS. At light …

Serotonin Transporter (5-Httlpr) Genotype And Childhood Trauma Are Associated With Individual Differences In Decision Making, Scott F. Stoltenberg, Melissa K. Lehmann, Cynthia Anderson, Parthasarathi Nag, Cheryl Anagnopoulos

Department of Psychology: Faculty Publications

The factors that influence individual differences in decision making are not yet fully characterized, but convergent evidence is accumulating that implicates serotonin (5-HT) system function. Therefore, both genes and environments that influence serotonin function are good candidates for association with risky decision making. In the present study we examined associations between common polymorph isms in the serotonin transporter gene (SLC6A4; 5-HTTLPR and rs25531)' the experience of childhood trauma and decision making on the Iowa gambling task (IGT) in 391 (64.5% female) healthy Caucasian adults. Homozygosity for the 5-HTTLPR L allele was associated with riskier decision making in the first block …

Adipose Tissue Abca1 Contributes To Hdl Biogenesis In Vivo, Soonkyu Chung, Janet K. Sawyer, Abraham K. Gebre, Nobuyo Maeba, John S. Parks

Department of Nutrition and Health Sciences: Faculty Publications

Background—Adipose tissue (AT) is the body’s largest free cholesterol (FC) reservoir and abundantly expresses ATP binding cassette transporter A1 (ABCA1), a key cholesterol transporter for HDL biogenesis. However, the extent to which AT ABCA1 expression contributes to HDL biogenesis in vivo is unknown.

Methods and Results—Adipocyte-specific ABCA1 knockout mice (ABCA1^−A/−A) were

generated by crossing ABCA1floxed mice with aP2 cre transgenic mice. AT from ABCA1^−A/−A mice had −^A/−A mice had a twofold increase in FC content, compared to WT mice, and failed to efflux cholesterol to apoA-I. …

Hydrogen Peroxide Probes Directed To Different Cellular Compartments, Mikalai Malinouski, You Zhou, Vsevolod V. Belousov, Dolph L. Hatfield, Vadim N. Gladyshev

Department of Biochemistry: Faculty Publications

Background: Controlled generation and removal of hydrogen peroxide play important roles in cellular redox homeostasis and signaling. We used a hydrogen peroxide biosensor HyPer, targeted to different compartments, to examine these processes in mammalian cells.

Principal Findings: Reversible responses were observed to various redox perturbations and signaling events. HyPer expressed in HEK 293 cells was found to sense low micromolar levels of hydrogen peroxide. When targeted to various cellular compartments, HyPer occurred in the reduced state in the nucleus, cytosol, peroxisomes, mitochondrial intermembrane space and mitochondrial matrix, but low levels of the oxidized form of the biosensor were also observed …

Targeted Deletion Of The Mouse Mitoferrin1 Gene: From Anemia To Protoporphyria, Marie-Berengere Troadec, David Warner, Jared Wallace, Kirk Thomas, Gerald J. Spangrude, John Phillips, Oleh Khalimonchuk, Barry H. Paw, Diane Mcvey Ward, Jerry Kaplan

Department of Biochemistry: Faculty Publications

Mitoferrin1 is 1 of 2 homologous mitochondrial iron transporters and is required for mitochondrial iron delivery in developing erythroid cells. We show that total deletion of Mfrn1 in embryos leads to embryonic lethality. Selective deletion of Mfrn1 in adult hematopoietic tissues leads to severe anemia because of a deficit in erythroblast formation. Deletion of Mfrn1 in hepatocytes has no phenotype or biochemical effect under normal conditions. In the presence of increased porphyrin synthesis, however, deletion of Mfrn1 in hepatocytes results in a decreased ability to convert protoporphyrin IX into heme, leading to protoporphyria, cholestasis, and bridging cirrhosis. Our results show …

The Effect Of Cooking On Diphacinone Residues Related To Human Consumption Of Feral Pig Tissues, William C. Pitt, Michelle Higashi, Thomas M. Primus

United States Department of Agriculture Wildlife Services: Staff Publications

We examined feral pig tissues to determine whether the potential hazard of consuming meat from pigs previously exposed to diphacinone rodenticide baits was reduced by cooking. Residue levels were measured in cooked and uncooked tissues of feral pigs exposed to sub-lethal quantities of the anticoagulant rodenticide. Pigs were provided large amounts of baits or untreated food to consume, then euthanized prior to the onset of symptoms indicative of rodenticide poisoning or sickness. For analysis, we grouped pigs into three levels of mean diphacinone consumption: 0, 3.5, and 7.4 mg/kg. None of the pigs displayed obvious signs of toxicity during the …

Chapter Nine: Understanding Declines In Rusty Blackbirds, Russell Greenberg, Dean W. Demarest, Steven M. Matsuoka, Claudia Mettke-Hofmann, David Evers, Paul B. Hamel, Jason Luscier, Luke L. Powell, David Shaw, Michael L. Avery, Keith A. Hobson, Peter J. Blancher, Daniel K. Niven

United States Department of Agriculture Wildlife Services: Staff Publications

The Rusty Blackbird (Euphagus carolinus), a formerly common breeding species of boreal wetlands, has exhibited the most marked decline of any North American landbird. North American Breeding Bird Survey (BBS) trends in abundance are estimated to be ‒12.5%/yr. over the last 40 years, which is tantamount to a >95% cumulative decline. Trends in abundance calculated from Christmas Bird Counts (CBC) for a similar period indicate a range-wide decline of ‒5.6%/yr. Qualitative analyses of ornithological accounts suggest the species has been declining for over a century. Several studies document range retraction in the southern boreal forest, whereas limited data …

Comparing A Bioenergetics Model With Feeding Rates Of Caged European Starlings, H. Jeffrey Homan, Randal S. Stahl, George M. Linz

United States Department of Agriculture Wildlife Services: Staff Publications

We tested a bioenergetics model integrated within a mortality model that estimates numbers of European starlings (Sturnus vulgaris) poisoned with the avicide, Compound DRC-1339 Concentrate. The bioenergetics model predicted daily metabolic rate. Accuracy and reliability of this variable is critical because other algorithms (e.g., toxicity regressions, feeding behavior) in the mortality model depend on metabolic rate to calculate the amount of DRC-1339 ingested per bird. We tested the bioenergetics model by comparing its estimates of metabolic rate with those generated from measuring feeding rates of caged starlings during a feeding trial conducted outdoors during January 2008. Over the …

Assessment Of Tannin Variation In Tamarisk Foliage Across A Latitudinal Gradient, Amanda M. Hussey, B. A. Kimball, Jonathan M. Friedman

United States Department of Agriculture Wildlife Services: Staff Publications

Certain phenotypic traits of plants vary with latitude of origin. To understand if tannin concentration varies among populations of tamarisk (Tamarix spp.) according to a latitudinal gradient, an analytical method was adapted from an enological tannin assay. The tannin content (wet basis) of tamarisk foliage collected from 160 plants grown in a common garden ranged from 8.26 to 62.36 mg/g and was not correlated with the latitude of the original North American plant collection site. Tannins do not contribute to observed differences in herbivory observed among these tamarisk populations.

Who Hits And Hoots At Whom? Potential For Interference Competition Between Barred And Northern Spotted Owls, Nicholas J. Van Lanen, Alan B. Franklin, Kathryn P. Huyvaert, Raoul F. Reiser Ii, Peter C. Carlson

United States Department of Agriculture Wildlife Services: Staff Publications

The northern spotted owl (Strix occidentalis caurina) is a controversial species in the Pacific Northwest that is listed as threatened under the Endangered Species Act. The barred owl (Strix varia), a species historically restricted to eastern North America, has recently expanded its range to completely overlap that of the northern spotted owl. Recent evidence suggests that barred owls may compete with northern spotted owls and may be one cause for recent declines in some northern spotted owl populations. Our focus was to examine whether barred owls have the potential to competitively exclude northern spotted owls from …

Efficacy Of Erl-4221 As An Ovotoxin For Feral Pigs (Sus Scrofa), Dustin L. Sanders, Fang Xie, Richard E. Mauldin, Jerome C. Hurley, Lowell A. Miller, Michelle R. Garcia, Randy W. Deyoung, David B. Long, Tyler A. Campbell

United States Department of Agriculture Wildlife Services: Staff Publications

Context. The expansion of feral pig populations across the United States has increased the occurrence of damage and damage complaints.Newtechniques are needed to more effectively manage feral pig damage, including the development of fertility control agents.

Aims. We aimed to assess the ovotoxic properties of ERL-4221 as a candidate fertility control agent for feral pigs.

Methods. We conducted two palatability trials to determine ERL-4221 acceptance and one experimental trial with ERL- 4221 at the captive wildlife facility of Texas A&M University-Kingsville during 2008. Our experimental trial had three treatments, a control containing no ERL-4221, baits containing 16.0 …

The Need To Quantify Ecosystem Services Provided By Birds, Daniel G. Wenny, Travis L. Devault, Matthew D. Johnson, Dave Kelly, Cagan H. Sekercioglu, Diana F. Tomback, Christopher J. Whelan

United States Department of Agriculture Wildlife Services: Staff Publications

What are the birds worth--what is their actual dollar value to human society? To most of us in the ornithological community, birds are invaluable. But in these times we need more specific rationales to convince policy makers and business leaders to include bird conservation in land-use and development decisions. Over the past two decades, awareness of our dependence on a variety of ecosystem services (natural ecological processes that benefit human society) and of their importance and prevalence has progressed toward the goal of making conservation a mainstream value. Building strategies for the protection of ecosystem services into conservation and land-use …

The Nebraska Deer Exchange: A Novel Program For Donating Harvested Deer, Aaron M. Hildreth, Scott Hygnstrom, Kit M. Hams, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Populations of white-tailed deer (Odocoileus virginianus) have become overabundant throughout their historic range, especially in developed landscapes. A variety of techniques, including controlled hunts in urban–suburban areas, are being used to increase harvest. Deer donation programs have been initiated in nearly all 50 states of the United States, and have been successful at providing venison to the hungry, but at significant costs to wildlife agencies and hunters. Our objectives were to: 1) develop a low-cost program to facilitate the exchange of excess harvested deer from hunters to recipients; 2) evaluate the attitudes of participating hunters and recipients; and …

A Field Evaluation Of A Trap For Invasive American Bullfrogs, Nathan P. Snow, Gary W. Witmer

United States Department of Agriculture Wildlife Services: Staff Publications

Native to the eastern United States, American bullfrogs (Rana catesbeiana [Lithobates catesbeianus]) have been introduced in many countries throughout the world. There have been relatively few effective and efficient control methods developed to manage bullfrogs. Particularly in the Hawaiian Islands, Pacific coast of North America, and Japan, finding effective methods for controlling invasive bullfrogs is needed with special emphasis on low impacts for sensitive native species. We conducted a field study to examine the efficacy of a newly designed live trap for capturing invasive bullfrogs. We found that our trap was successful at capturing bullfrogs because we …

Artificial Nest Cavity Used Successfully By Native Species And Avoided By European Starlings, Laura A. Tyson, Bradley F. Blackwelll, Thomas W. Seamans

United States Department of Agriculture Wildlife Services: Staff Publications

We describe a weather-durable cavity design used successfully by cavity-nesting species native to the eastern USA and, although accessible, avoided by European Starlings (Sturnus vulgaris). The artificial nest cavity was constructed using 9.5-cm inside diameter polyvinyl chloride tubes cut to 27.5-cm lengths. The tubes were mounted horizontally with 5.1- cm entry holes drilled through one of the capped ends. Eastern Bluebirds (Sialia sialis), House Wrens (Troglodytes aedon), and Tree Swallows (Tachycineta bicolor) nested in 49 of 100, newly mounted tubes on utility poles in north-central Ohio, USA from April through June 2009. …

Vertebrate Species Introductions In The United States And Its Territories, Gary W. Witmer, Pam L. Fuller

United States Department of Agriculture Wildlife Services: Staff Publications

At least 1,065 introduced vertebrate species have been introduced in the United States and its territories, including at least 86 mammalian, 127 avian, 179 reptilian/amphibian, and 673 fish species. Examples in each major taxonomic group include domestic cat, small Indian mongoose, red fox, goat, pig, rabbit, rats, house mouse, gray squirrel, nutria, starling, Indian common myna, red-vented bulbul, brown treesnake, red-eared slider, brown trout, tilapia, and grass carp. We briefly review some of these species and the types of damage they cause. We then review the basic types of methods used for control or eradication of each taxonomic group, including …

Edison State College General Catalog 2010-2011, Edison State College

FSW Course Catalogs

Edison State College General Catalog Volume Forty Nine. 2010-2011. Scanned by Internet Archive.

Serum Monocyte Chemoattractant Protein-1 In Pancreatic Cancer., Jennifer Sullivan, Qiaoke Gong, Terry Hyslop, Harish Lavu, Galina Chipitsyna, Charles Yeo, Hwyda A Arafat

Department of Surgery Faculty Papers

Background/Aims. Pancreatic ductal adenocarcinoma (PDA) has etiological association with chronic inflammation. Elevated circulating levels of inflammatory mediators, such as monocyte chemoattractant protein-1 (MCP-1), are found in obese individuals. We hypothesized that serum MCP-1 levels are elevated in obese PDA patients. Methods. ELISA was used to analyze MCP-1 serum levels in PDA (n = 62) and intraductal papillary mucinous neoplasms (IPMN) (n = 27). Recursive partitioning statistical analysis investigated the relationship between log MCP-1 and clinicopathological parameters. Results. Log MCP-1 values were significantly (P < 0.05) elevated in patients with BMI ≥ 37.5. In patients with BMI < 37.5, average log MCP-1 values were significantly elevated in PDA patients when compared to IPMN patients. Within the IPMN group, higher log MCP-1 levels correlated with increased age. Recursive partitioning analysis of IPMN versus PDA revealed a strategy of predicting characteristics of patients who are more likely to have cancer. This strategy utilizes log MCP-1 as the primary factor and also utilizes smoking status, gender, and age. Conclusion. MCP-1 is a promising biomarker in pancreatic cancer. The potential of using MCP-1 to distinguish PDA from IPMN patients must be studied in larger populations to validate and demonstrate its eventual clinical utility.