Digital Commons Network^™

The Role Of Trm9 In Stress Responses, Ashish Ravindra Patil

Legacy Theses & Dissertations (2009 - 2024)

Cells need to respond appropriately to environmental changes in order to maintain homeostasis. The cellular response to an environmental stress is regulated at transcriptional, translational and post translational levels. The tRNA, which acts as an adaptor molecule between the mRNA and the protein, plays an important role in the translational regulation of cellular responses to stress and is one of the most heavily modified biomolecules. In Saccharomyces cerevisiae , the wobble uracil of the tRNA(3'-UCU-5') Arg, tRNA(3'-UUC-5') Glu and certain other specific tRNAs are modified to 5-methoxycarbonylmethyluridine (mcm5U) and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) residues by the tRNA methyltransferase 9 (Trm9). Modifications at …

Maternal Caffeine Consumption And Risk Of Congenital Limb Deficiency Malformations, Lei Chen

Legacy Theses & Dissertations (2009 - 2024)

ABSTRACT

Regulation Of The Aryl Hydrocarbon Receptor And The Signal Transducer And Activator Of Transcription 1 By Long-Term Estrogen Exposure In Breast Carcinoma Cells, Neal Englert

Legacy Theses & Dissertations (2009 - 2024)

The risk of developing breast cancer is known to be associated with a woman's lifetime exposure to estrogens, both endogenous and exogenous. Increased exposure to estrogens stimulates cellular proliferation, which is a widely accepted theory of estrogen receptor positive mammary carcinogenesis. The molecular mechanisms of gene expression regulation in response to long-term estrogen exposure (LTEE) of MCF-7 breast cancer cells were addressed in this study, with a focus on the aryl hydrocarbon receptor (AHR) and signal transducer and activator of transcription 1 (STAT1).

Physiology Of Yawning : Proximate Mechanisms Supporting An Ultimate Function, Melanie Lee Shoup-Knox

Legacy Theses & Dissertations (2009 - 2024)

Recent research suggests that yawning functions to cool the brain during periods of mild hyperthermia. Evidence for this hypothesis is largely behavioral, and includes reports of increased yawning during increases in ambient temperature and times of stress as well as an amelioration of yawning upon nasal breathing and forehead cooling. Little research has been published on the physiological mechanisms supporting a brain cooling function, however. The current set of studies explores human and animal physiological parameters in search of evidence of brain cooling during yawning. In humans, heart rate, skin temperature, and skin conductance findings suggest that yawning involves an …

Effect Of Parent Personal Experience On Views, Level Of Concern, And Reactions To Middle School Students' Bullying Behavior, Leigh A. Cooper

Legacy Theses & Dissertations (2009 - 2024)

This study sought to generate a base of literature pertaining to parent historical experiences with bullying. Specifically, parent history of bullying was examined as a predictor of current perceptions, level of concern, and strategies used to prevent and intervene with bullying for middle school students. Parents from a public school district in the Northeast were invited to complete an online survey through letters distributed to their student and media-based announcements. Two-hundred-sixty participants provided viable responses. A series of hierarchical regression analyses were used to determine if parent history of involvement in bullying was predictive of their current responses to bullying.

The P38 Alpha Stress-Activated Protein Kinase Pathway In Mammary Gland Development And Cancer, Huei-Chi Wen

Legacy Theses & Dissertations (2009 - 2024)

Breast tumors arise from preinvasive lesions consisting of abnormal epithelial cells that accumulate within the mammary duct. However, the initiating steps and underlying pathways leading to the formation of these early stages of breast carcinoma, such as ductal carcinoma in situ (DCIS), is not fully understood.

Examining The Role Of Ethnicity In The Nature And Assessment Of Body Image In Men, Jason Matthew Lavender

Legacy Theses & Dissertations (2009 - 2024)

Although body image research has historically focused on dissatisfaction among women, studies within the past decade have begun to focus on body dissatisfaction among men, revealing important gender differences in the nature, conceptualization, and assessment of this construct. Despite recent advances in the research literature in this area, few studies have examined ethnic differences in male body image, and no information is available on the psychometric properties of many of the most widely used body image measures for samples of ethnic minority men. The purpose of the present research was to evaluate the psychometric properties of the Drive for Muscularity …

Advanced Statistical Methods For Biochemical And Forensic Applications Of Raman Spectroscopy, Vitali Sikirzhytski

Legacy Theses & Dissertations (2009 - 2024)

This work is dedicated to the development and application of advanced statistical methods for biochemical and forensic applications of Raman spectroscopy. We developed a new concept of multidimensional Raman signatures for forensic identification of body fluid traces, which was successfully applied to the study of dried blood, semen, saliva, sweat and vaginal fluid stains. Several alternative advanced chemometric techniques were also successfully tested for the problems of pure body fluids discrimination and mixture analysis. Studies of body fluid mixtures with low blood and semen concentrations indicated that the detection limit of a minor contributor was as low as a few …

Unstoppable? : A Closer Look At Terrorism Displacement, Henda Yao Hsu

Legacy Theses & Dissertations (2009 - 2024)

Since T.A Repetto first introduced crime displacement in 1976, situational crime prevention researchers have long been challenged by the assumption that displacement is an unavoidable consequence of focused crime prevention measures. Despite evidence that criminals do not inevitably shift their offending behavior in response to crime prevention initiatives, recent efforts to extend situational prevention to terrorism have been met with criticisms of displacement. This study examines whether terrorists displace their attacks by changing their methods once the opportunities to carry out acts of terrorism are blocked. To accomplish this, the author examines, through the use of the newly synthesized Global …

Structure And Function Of Coding And Non-Coding Rna Domains, Fei Liu

Legacy Theses & Dissertations (2009 - 2024)

As a versatile molecule, RNA exhibits an astonishing variety of functional activities, which is typically attributed to its particular structure-forming capacity. There are an increasing number of established roles and systems where RNA structures, in particular, RNA pseudoknots, affect specific biological processes based on their structural features. The structure-function-relationships of RNA in three systems has been invesgated: the telomerase RNA pseudoknot domain from S. cerevisiae, a poteintial unusual H-type pseudoknot forming region near the 3'-splice-site in the influenza virus NS1 mRNA, and the 5'-untranslated-region of the CC16 mRNA where mutations in the wildtype sequence have been related to asthma. UV-monitored …

Evaluating How New York Natural Heritage Program Information Affects Development Projects In New York State, Tara Salerno

Legacy Theses & Dissertations (2009 - 2024)

Is New York Natural Heritage Program (NYNHP) information used to affect land-use decisions in New York State? A seemingly simple question, yet it had no answer. The NYNHP maintains a comprehensive database on the status and location of rare species and significant natural communities throughout New York State. The NYNHP uses this database to provide information to consulting firms, project applicants, developers, local governments, state and federal agencies, private organizations, non-profits, the environmental conservation community, and the general public (together referred to as requestors) with the intent that such information will be used to aid in land-use decisions that will …

Synthesis And Characterization Of Diallylic Polysulfanes And Study Toward The Synthesis Of Thiarubrine E, Kai Wang

Legacy Theses & Dissertations (2009 - 2024)

Garlic oil contains diallyl polysulfanes as major components, which have been used as environmentally benign bird repellants and nematicides. While previous studies found that the biological activity of polysulfanes increases with numbers of sulfur atoms, higher diallyl polysulfanes are currently unavailable. A simple approach was developed, using liquid sulfur as a reagent, to the synthesis of diallyl polysulfanes containing chains of more than 20 sulfur atoms. This method was successfully applied to other diallylic disulfides and to garlic oil to give new families of polysulfanes. Some diallyl polysulfanes were isolated in a pure form by preparative high performance liquid chromatography …

Applications Of Raman Spectroscopy For Silicon Stress Characterization In Integrated Circuits, Colin Mcdonough

Legacy Theses & Dissertations (2009 - 2024)

The introduction of mechanical stress in Si-based integrated circuits (ICs), whether desired or undesired, is intrinsic to IC fabrication. The origins are diverse and result from the numerous materials, geometries, and processes involved in fabrication. These stresses can lead to such effects as delamination, void formation and migration, and fracture, and can significantly affect device performance. As a result, stress development is a major concern for reliability, process control, and device design. It is necessary to investigate and characterize the origins and levels of the induced stresses. A more complete fundamental understanding of the evolution of stress in ICs and …

The Value Of Human Life : An Individual-Level Determinant Of Violence, Maria Tcherni

Legacy Theses & Dissertations (2009 - 2024)

My contribution to the study of causes of violence in criminology is introducing a theoretical concept of the Value of Life (VL) and developing an empirical solution to measuring it. The value that an individual implicitly assigns to the human life - both his/her own life and other people's lives - is a theoretical concept that synthesizes information about precursors, correlates, and determinates of violence. The VL concept provides a meaningful way to explain some phenomena not commonly associated with violent behavior (for example, reckless behavior with regard to one's health), while also illuminating some puzzling associations consistently found in …

Herpes Simplex Virus Type 1 : Interactions With The Cellular Proteasome And Microrna Pathways, Daniel Joseph Munson

Legacy Theses & Dissertations (2009 - 2024)

Herpes Simplex Virus Type 1 (HSV-1) is a human pathogen that is estimated to have infected 60-80% of the population worldwide, causing oral lesions, encephalitis, and blindness. Infection results in life-long latency of the virus, with periods of reactivation and symptomatic disease. Early in infection, HSV-1 induces the formation of replication compartments and VICE (virus induced chaperone enriched) domains within the nucleus. Adjacent to VICE domains, we observed foci which contained Mss1, a cellular protein normally associated with the proteasome. Mss1 belongs to the AAA-ATPase family of proteins, which have been shown to function in DNA repair, transcription, translation, and …

Typically Developing Children's Attitudes Towards A Peer With Behaviors Indicative Of Autism, Lindsay A. Washington

Legacy Theses & Dissertations (2009 - 2024)

Although inclusive educational programming offers opportunities for increased contact between children with autism spectrum disorders (ASD) and typically developing peers, research indicates that children with ASD continue to struggle socially in these settings. One possible contributing factor could be the type of attitudes that typically developing peers hold regarding children with ASD. The purpose of the present study was to determine whether typically developing first grade children express rejecting attitudes towards a hypothetical peer with behaviors indicative of autism. This study employed a between-groups design and included 89 participants. Participating children viewed a brief video that depicted either a child …

Advocating For Children In Care In A Climate Of Economic Recession: The Relationship Between Poverty And Child Maltreatment, Bruce A. Boyer, Amy E. Halbrook

Northwestern Journal of Law & Social Policy

No abstract provided.

Design And Application Of Composite Rna Aptamers, Shengchun Wang

Legacy Theses & Dissertations (2009 - 2024)

aptamers are being developed as an essential tool in many fields of biological research. Their utility is not limited to being protein inhibitors; a lot of novel functions can be realized. However, in vivo application of RNA aptamers still faces many challenges. The aim of this dissertation is to design and apply composite aptamers in multiple expression and delivery systems to address some critical issues, such as correct folding, high level production, degradation by nucleases, excessive consumption of cellular resource and potential toxic effect.

Dietary Restraint And Weight Loss In College Students As Risk Factors For Eating Pathology, Katherine Elizabeth Schaumberg

Legacy Theses & Dissertations (2009 - 2024)

Many suggest that dietary restraint represents a key component to the etiology and maintenance of eating pathology, and much research supports this position. Some recent evidence brings to question the relationship between dietary restraint and eating disorder risk. Furthermore, measures of dietary restraint do not appear to consistently predict caloric restriction, and these scales appear inadequate for differentiating between healthy and risky restraint in individuals. The current study seeks to examine the relationship between self-reported dietary restraint, recent weight loss, and eating pathology in a college sample to determine if cognitive restraint measured by restraint scales coupled with caloric restriction …

Homologous And Heterologous Crystallin Protein Interactions As The Molecular Basis Of Inherited Cataract, Priya Ranjan Banerjee

Legacy Theses & Dissertations (2009 - 2024)

Age-related cataract is the most common cause of blindness worldwide. Nearly fifty percent of Americans above the age of 75 are diagnosed with this disease (http://www.nei.nih.gov/eyedata/pbd_tables.asp), and surgical intervention is the sole method of treatment at present (http://www.nei.nih.gov/healthyvision/objective/cataracts.asp). In the developing world, even this treatment is not readily available. These are compelling reasons to search for better treatments to delay, prevent or arrest cataract formation. Recent evidence suggests that age-related cataracts also have a genetic component. Therefore, determining the mechanisms underlying genetic cataracts with a known association to a protein-mutation is one important strategy towards understanding the molecular basis for …

Biophysical Investigations Of The Molecular Basis Of Cataract Associated With The R76s Mutation In Human Gammad-Crystallin, Vurghun Ahmadov

Legacy Theses & Dissertations (2009 - 2024)

Cataract disease results when the eye lens becomes opaque and scatters a significant part of the incoming light into the eye. The lens contains very high concentrations of the lens proteins, called crystallins, which are present at concentrations comparable to those found in protein crystals (about 400-600 mg/mL). Chemical modifications of the crystallins, such as oxidation and deamidation, or genetic mutations are known to result in increased light-scattering in vitro, and are implicated in cataract formation in vivo. Here we present the in vitro work on a mutant protein of human gammaD crystallin (HGD), namely R76S (i.e. Arg 76 to …

Features Of The Human Body And Voice : The Biological Impact On Perception And Attraction Through Multiple Sensory Domains, Roy Nathan Pipitone

Legacy Theses & Dissertations (2009 - 2024)

A growing body of literature has shown that variation in the sound of a person's voice conveys important information about our underlying biology. The present studies further investigate how the human voice can be used as a medium that disseminates characteristics related to mate quality and attraction. The human voice has been shown to be affected by cyclic hormonal fluctuations across the menstrual cycle, and raters show preferences for voice recordings at heightened fertility times compared to other times during the menstrual cycle. In the current studies, this finding was replicated multiple times and less masculine males were found to …

Bringing The Dead Compartment Of A Plant Cell To Life: A Novel Imaging Technique Resurrects The Dynamic Nature Of The Apoplast, Doug Van Hoewyk

Biology

No abstract provided.

Assessing Hormones In Response To Trauma, Corina E. Klein

Honors Capstones

Hormones produced in the body may be important in ameliorating the effects of traumatic stress. Previous research bas shown that the concentration of several hormones, such as cortisol, dehydroepiandrosterone (DHEA) and DHEA-sulfated (DHEAS) in a given individual who has been exposed to a traumatic event may be indicative of the likelihood that the individual will develop posttraumatic stress disorder (PTSD). The current study assessed whether higher levels of cortisol in comparison to DHEA or DHEAS could indicate a predisposition toward future mental illness following an emotionally stressful event. Female students from Northern lllinois University provided salivary samples before and following …

Isolation And Analysis Of Arabidopsis Thaliana Lines Bearing Drg Knock-Out Mutations And Overexpressing Gfp Protein Fusions: Are Wild-Type Drgs Necessary For Heat Stress Granule Formation?, Lindsey Mckinney

Honors Capstones

Purpose and Methodology: The purpose of this study was to determine the role of DRG and GFP in response to heat stress in Arabidopsis by examining whether any DRG mutant combination inhibits the formation of heat stress granules (HSGs) following exposure of plants to heat stress. Methods utilized in the experimentation include the polymerase chain reaction (PCR) and gel electrophoresis to screen for plants containing the desired combination of genes, a root-growth assay to study the relationship between phenotype and genetic composition, and confocal microscopy to observe the formation of HSGs in root tips. Findings: The genetic composition of DRG …

Miltown: An American Antidepressant, 1945-1955, Alexander Porter

Honors Capstones

Out of 2.4 billion drugs prescribed throughout America in 2005, antidepressants accounted for 118 million of them. Family doctor Ronald Dworkin, one of many critics of antidepressant use in America, argues that doctor's are now "medicating unhappiness."! However, his blanket statement completely overlooks the history of antidepressant use in the United States. The use of prescription drugs to treat "unhappiness" is not a new phenomenon. Before modern antidepressants, tranquilizers such as Librium and Valium were used to treat outpatient-based mental illness. Although they reaped large profits, they also attracted social and political attention. But even these were not the first …

The Role Of Nitric Oxide In Pancreatic Cell Secretion, Kaylee R. Walters

Honors Capstones

The pancreas is a model tissue that has been extensively studied in the lab of my research supervisor. They have shown that nitric oxide is produced by the pancreas and is necessary for sectretion; however, the mechanism of action remained to be identified. The goal of my research over the past year was to investigate the signaling role of nitric oxide, the peroxynitrite producing enzyme NADPH oxidase NOX-4, and the nitrosylation oftarget proteins on the zymogen granule membrane as a potential mechanism to regulate cytoskeletal reorganization and migration of the secretory vesicles. In addition, I performed research to understand the …

Floristic And Environmental Characteristics Of Sub-Boreal Peatlands Of Minnesota And Western Wisconsin, Kevin Douglas Clement

All Graduate Theses, Dissertations, and Other Capstone Projects

Peatland communities in the sub-boreal region of Minnesota and western Wisconsin were characterized by floristic composition, structure and environmental characteristics to determine the natural variation among peatland communities in the region and their shared associations with the boreal and temperate peatlands of North America. Floristic classification revealed five, distinct peatland community types, identified as Sphagnum Bogs, Herbaceous Fens, Forested Fens, Rich/Calcareous Fens and Phalaris anrundinacea dominated peatlands. Differences among sub-boreal peatlands largely reflected changes in the significant indicator and dominant plant species identified, which were found to exhibit high fidelities to a particular peatland community type. Floristic variations among sub-boreal …

January 2011 Newsletter, Career Center, Illinois Wesleyan University

Career Connections Newsletter

Issue Highlights:

• Alumni Profile of Robert Rave, Class of 1996: Publicist and Author of Spin and Waxed
• The Realities of a Changing Job Market
• Cleaning Up Your Online Presence: Why and How?

Pulsed Radiofrequency Neuromodulation Of Peripheral Nerve Injury, Alex Willett

Honors Projects

Pulsed-radiofrequency neuromodulation (PRF) is a pain management technique that involves placing a needle electrode near nerves and generating electrical current pulses in order to modulate the transduction of somatosensory information through those nerves. This technique evolved from a similar radiofrequency (RF) procedure in which constant current is distributed to a nerve or neural structure. RF interrupts nerve conduction and prevents somatosensory information from reaching the brain. In the case of continuous radiofrequency, however, the destructive lesion can cause further complications and unwanted side effects. According to research, PRF, unlike RF, is non-destructive yet still induces analgesia and consequently represents a …