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2011

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Articles 14521 - 14550 of 20343

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Association Of Plasminogen Activator Inhibitor-1 And Tissue Plasminogen Activator With Type 2 Diabetes And Metabolic Syndrome In Malaysian Subjects, Z. Al-Hamodi, I.S. Ismail, R. Saif-Ali, K.A. Ahmed, S. Muniandy Jan 2011

Association Of Plasminogen Activator Inhibitor-1 And Tissue Plasminogen Activator With Type 2 Diabetes And Metabolic Syndrome In Malaysian Subjects, Z. Al-Hamodi, I.S. Ismail, R. Saif-Ali, K.A. Ahmed, S. Muniandy

Research Publications (2011 to 2015)

Background: Increased plasma plasminogen activator inhibitor-1 (PAI-1) activity and decreased tissue plasminogen activator (tPA) activity could be considered a true component of the metabolic syndrome (MetS) associated with an increased risk of developing cardiovascular diseases (CVD) and fibrinolytic abnormalities. The aim of this study was to investigate the association of tPA and its inhibitor PAI-1 with type 2 diabetes (T2D) and MetS and interrelationship between PAI-1 and tPA activities and antigens in Malaysian T2D and normal subjects. Methods: The plasma activities and antigens of PAI-1 and tPA and the levels of the tPA/PAI-1 complex as well as serum insulin, parameter …

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Bioelectrical Impedance Analysis Device: Measurement Of Bioelectrical Tissue Conductivity In Dengue Patients, H. Abdul Rahim, M.N. Taib, F. Ibrahim, Z. Abdul Rahim Jan 2011

Bioelectrical Impedance Analysis Device: Measurement Of Bioelectrical Tissue Conductivity In Dengue Patients, H. Abdul Rahim, M.N. Taib, F. Ibrahim, Z. Abdul Rahim

Research Publications (2011 to 2015)

This paper describes and introduces a new approach to monitor dengue hemorrhagic fever using BIA technique. This technique is an in vivo technique where an approximately a small average constant current of 460 μA at single frequency, 50 kHz is applied through human body, and measure the body's resistance and reactance to that current via four-surface electrode. These measurements are combined with the patient data (i.e. age, sex, height and weight) to estimate mass and water compartments. The dengue's clinical studies in correlation with the BIA measurement have been conducted on 119 male and 91 female who are the DHF …

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Biochemical And Toxinological Characterization Of Naja Sumatrana (Equatorial Spitting Cobra) Venom, M.K.K. Yap, N.H. Tan, Shin Yee Fung Jan 2011

Biochemical And Toxinological Characterization Of Naja Sumatrana (Equatorial Spitting Cobra) Venom, M.K.K. Yap, N.H. Tan, Shin Yee Fung

Research Publications (2011 to 2015)

The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A2. Fractionation of N. sumatrana venom by Resource® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice. Most of the protein peaks …

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Capsular Serotyping Of Pasteurella Multocida From Various Animal Hosts - A Comparison Of Phenotypic And Genotypic Methods, N.D. Arumugam, N. Ajam, P.J. Blackall, N.M. Asiah, M. Ramlan, J. Maria, S. Yuslan, Kwai Lin Thong Jan 2011

Capsular Serotyping Of Pasteurella Multocida From Various Animal Hosts - A Comparison Of Phenotypic And Genotypic Methods, N.D. Arumugam, N. Ajam, P.J. Blackall, N.M. Asiah, M. Ramlan, J. Maria, S. Yuslan, Kwai Lin Thong

Research Publications (2011 to 2015)

One hundred and fourteen strains of Pasteurella multocida were isolated from different domestic animals species (cattle, buffalo, sheep, goat, pig, rabbit, dog, cat), avian species (chicken, duck, turkey) and wild animals (deer, tiger, orang utan, marmoset). The serogroups of P multocida were determined by both conventional capsular serotyping and a multiplex PCR assay targeting specific capsular genes. Based on the conventional serotyping method, the 114 strains of P multocida were subtyped into 55 species-specific (untypeable strains) P. multocida, 15 serogroup A, 23 serogroup B and 21 serogroup D. Based on the multiplex PCR assay on the specific capsular genes associated …

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Change In Facial Shape In Two Cohorts Of Japanese Adult Female Students Twenty Years Apart, M.G. Hossain, A. Saw, F. Ohtsuki, P.E. Lestrel, Tunku Kamarul Jan 2011

Change In Facial Shape In Two Cohorts Of Japanese Adult Female Students Twenty Years Apart, M.G. Hossain, A. Saw, F. Ohtsuki, P.E. Lestrel, Tunku Kamarul

Research Publications (2011 to 2015)

Introduction: Secular changes in the head and body dimensions of the Japanese population were recorded during the last century, but studies on Japanese adult facial shape were poorly documented. This study aimed to document the secular changes in facial shape and their association with craniofacial measures of Japanese adult female students. Methods: Data were collected from 1998-2001 and 1975-1979 from institutions in Tokyo and Kyoto. The prosopic index (PI) was derived from morphological facial height (n-gn) and bizygomatic breadth (zy-zy). Multiple regression analysis was used to determine the association between PI (facial form) and craniofacial measures. Results: Larger mean values …

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Change Point Detection Of Eeg Signals Based On Particle Swarm Optimization, M.F. Mohamed Saaid, Wan Abu Bakar Wan Abas, Hamzah Arof, N. Mokhtar, R. Ramli, Z. Ibrahim Jan 2011

Change Point Detection Of Eeg Signals Based On Particle Swarm Optimization, M.F. Mohamed Saaid, Wan Abu Bakar Wan Abas, Hamzah Arof, N. Mokhtar, R. Ramli, Z. Ibrahim

Research Publications (2011 to 2015)

This paper proposes a change point detection for electroencephalograms (EEG) signal application based on Particle Swarm Optimization (PSO). As EEG signal is well known consider as non-stationary in nature, we model the signal by using the sinusoidal-Heaviside function, which are capable to represent the change of the behavior of the signal. The parameter of the model with the change point location can be tuned by finding the minimum value of sum squared error. It was showed that the minimum value of sum squared error in the parameter tuning give the exact location of change point. The proposed method is applied …

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Chemically Induced Breast Tumors In Rats Are Detectable In Early Stages By Contrast Enhanced Magnetic Resonance Imaging But Not By Changes In The Acute-Phase Reactants In Serum, S. Golbabapour, W.W. Pang, J. George, T. Pasupati, P.S. Abdul-Rahman, Onn Haji Hashim Jan 2011

Chemically Induced Breast Tumors In Rats Are Detectable In Early Stages By Contrast Enhanced Magnetic Resonance Imaging But Not By Changes In The Acute-Phase Reactants In Serum, S. Golbabapour, W.W. Pang, J. George, T. Pasupati, P.S. Abdul-Rahman, Onn Haji Hashim

Research Publications (2011 to 2015)

The present study was undertaken to develop a rat model for monitoring the early development of breast cancer. Twelve female rats were divided into two groups of six rats that were either treated with N-methyl-N-nitrosourea to induce breast cancer or with bacterial lipopolysaccharide to induce inflammation. Serum samples taken from the rats prior to the treatment were used as controls. By the 14th week, presence of the tumor was detectable by contrast enhanced magnetic resonance imaging and confirmed by histopathology. When the serum proteins of the rats were examined by 2-dimensional electrophoresis (2-DE), no difference could be detected in the …

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Cloning And Characterisation Of Acc Oxidase Gene From Mas (Aa) Banana, W. Kunasekaran, Amru Nasrulhaq Boyce, C. Somasundram Jan 2011

Cloning And Characterisation Of Acc Oxidase Gene From Mas (Aa) Banana, W. Kunasekaran, Amru Nasrulhaq Boyce, C. Somasundram

Research Publications (2011 to 2015)

Considerable experimental attention has been given to amplify Mas (AA) ACC oxidase gene from Mas (AA) banana (Genbank accession number: EU106081). ACO gene from Mas (AA) has been compared with other homologous gene by using bioinformatics tools to study the characteristics of the gene such as evolutionary history, binding sites, predicted 3D structure and consensus region of the homologous proteins. The finding shows that Mas ACO is member of iron/ascorbate-dependent oxidase superfamily which requires ascorbate and iron for full activity so that it will meet the all the criteria requires as it is expressed in vivo to produce ethylene.

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Comparative Pcr-Based Fingerprinting Of Vibrio Cholerae Isolated In Malaysia, C.S.J. Teh, Kwai Lin Thong, R. Osawa, K.H. Chue Jan 2011

Comparative Pcr-Based Fingerprinting Of Vibrio Cholerae Isolated In Malaysia, C.S.J. Teh, Kwai Lin Thong, R. Osawa, K.H. Chue

Research Publications (2011 to 2015)

Vibrio cholerae, the causative agent of cholera, is endemic in many parts of the world, especially in countries poor in resources. Molecular subtyping of V. cholerae is useful to trace the regional spread of a clone or multidrug-resistant strains during outbreaks of cholera. Current available PCR-based fingerprinting methods such as Random Amplified Polymorphic DNA (RAPD)-PCR, Enterobacterial Repetitive Intergenic Consensus Sequence (ERIC)-PCR, and Repetitive Extragenic Palindromic (REP)-PCR were used to subtype V. cholerae. However, there are problems for inter-laboratory comparison as these PCR methods have their own limitations especially when different PCR methods have been used for molecular typing. In this …

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Comparison Study Of The Transradial Prosthetics And Biological Hand Using Motion Analysis Approach, N.A. Abd Razak, Noor Azuan Abu Osman, Wan Abu Bakar Wan Abas, A.N. Ahmad Fu'ad Jan 2011

Comparison Study Of The Transradial Prosthetics And Biological Hand Using Motion Analysis Approach, N.A. Abd Razak, Noor Azuan Abu Osman, Wan Abu Bakar Wan Abas, A.N. Ahmad Fu'ad

Research Publications (2011 to 2015)

This paper presents the comparison study of the motion analysis between the transradial prosthetics and the biological hand that focuses on the supination/pronation and flexion/extension movements. The design of the new transradial prosthetics is based on combining the new approach on both body-powered prosthetics and electric-powered prosthetics. Two servo motors were used to generate the transradial motion while the shoulder ultrasonic sensor functioned as the input for the biomechatronics system. This paper briefly describes the kinetics and kinematics data of motion analyses that focus on the degree of maximum and minimum rotation of each motion. The data analysis of the …

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Cyclization Vs. Cyclization/Dimerization In O-Amidostilbene Radical Cation Cascade Reactions: The Amide Question, Azhar Ariffin, N.F. Thomas, C.H. Kee, H. Morita, K. Awang, I. Noorbatcha, K. Takeya, C.G. Lim Jan 2011

Cyclization Vs. Cyclization/Dimerization In O-Amidostilbene Radical Cation Cascade Reactions: The Amide Question, Azhar Ariffin, N.F. Thomas, C.H. Kee, H. Morita, K. Awang, I. Noorbatcha, K. Takeya, C.G. Lim

Research Publications (2011 to 2015)

The n-butyramido, isobutyramido, benzamido, and furancarboxamido functions profoundly modulate the electronics of the stilbene olefinic and NH groups and the corresponding radical cations in ways that influence the efficiency of the cyclization due presumably to conformational and stereoelectronic factors. For example, isobutyramido-stilbene undergoes FeCl(3) promoted cyclization to produce only indoline, while n-butyramidostilbene, under the same conditions, produces both indoline and bisindoline.

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Determination Of Polymorphism At Ribosomal Dna In Hybrid Crosses Between Pleurotus Citrinopileatus And Schizophyllum Commune By Mating Technique, A.G. Rosnina, N. Abdullah, S. Vikineswary, S. Bhassu, F.A. Avin Jan 2011

Determination Of Polymorphism At Ribosomal Dna In Hybrid Crosses Between Pleurotus Citrinopileatus And Schizophyllum Commune By Mating Technique, A.G. Rosnina, N. Abdullah, S. Vikineswary, S. Bhassu, F.A. Avin

Research Publications (2011 to 2015)

Mushroom production is dependent on the quality of the spawn used to inoculate the fruiting substrates. New strains with the desired traits can be produced by mating technique involving fusion of two genes from two different mushroom type that controls the formation of dikaryon. Mating incompatibility in mushroom fungi is controlled by the mating-type loci. In tetrapolar species, two unlinked mating-type loci exists (A and B), whereas in bipolar species there is only one locus. The A and B mating-type loci encodes homeodomain transcription factors and pheromones and pheromone receptors, respectively. Like most mushroom species, both P. citrinopileatus and S. …

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Determination Of Toxinotypes Of Environmental Clostridium Perfringens By Polymerase Chain Reaction, L.C.H. Florence, S.L. Hakim, M.A. Kamaluddin, Kwai Lin Thong Jan 2011

Determination Of Toxinotypes Of Environmental Clostridium Perfringens By Polymerase Chain Reaction, L.C.H. Florence, S.L. Hakim, M.A. Kamaluddin, Kwai Lin Thong

Research Publications (2011 to 2015)

Toxinotype of Clostridium perfringens (CP) isolates collected from the Bernam River, Selangor River and Tengi Canal between April 2007 and January 2008 were determined by Polymerase Chain Reaction (PCR) using published primers. All the 147 isolates were toxinotype Type A, harbouring the alpha toxin gene. In addition, 5 of the isolates also had the enterotoxin (CPE) gene.

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Different Chemically Substituted Chitooligosaccharides Inhibit Beta-Secretase Activity, A.B.A. Ahmed, N.Y. Yoon, R.M. Taha, S.K. Kim Jan 2011

Different Chemically Substituted Chitooligosaccharides Inhibit Beta-Secretase Activity, A.B.A. Ahmed, N.Y. Yoon, R.M. Taha, S.K. Kim

Research Publications (2011 to 2015)

Chitooligosaccharide or COS is a kind of oligosaccharide that is an integral part of the nervous system. COS is synthesized by chemical substitution and derived AE-COS, DEAE-COS and DMAE-COS that are being shown on IC(50) value. AE-COS and DMAE-COS exhibited four fold less inhibition than DEAE-COS. In addition, non-competitive inhibitor was identified via a Dixon plot and the Ki inhibition constant (100 mu g/mL). We declared, chemical substitution of COS is a water soluble human safe BACE-1 inhibitor.

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Dna Strand Patterns On Aluminium Thin Films, N.M. Khatir, S.M. Banihashemian, V. Periasamy, Wan Haliza Abd Majid, S.A. Rahman, F. Shahhosseini Jan 2011

Dna Strand Patterns On Aluminium Thin Films, N.M. Khatir, S.M. Banihashemian, V. Periasamy, Wan Haliza Abd Majid, S.A. Rahman, F. Shahhosseini

Research Publications (2011 to 2015)

A new patterning method using Deoxyribose Nucleic Acid (DNA) strands capable of producing nanogaps of less than 100 nm is proposed and investigated in this work. DNA strands from Bosenbergia rotunda were used as the fundamental element in patterning DNA on thin films of aluminium (Al) metal without the need for any lithographic techniques. The DNA strands were applied in buffer solutions onto thin films of Al on silicon (Si) and the chemical interactions between the DNA strands and Al creates nanometer scale arbitrary patterning by direct transfer of the DNA strands onto the substrate. This simple and cost-effective method …

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Effect Of Elemental Sulfur Application On Ammonia Volatilization From Surface Applied Urea Fertilizer To Calcareous Sandy Soils, A.A. Soaud, M.E. Saleh, K.A. El-Tarabily, Mohd Sofian-Azirun, M.M. Rahman Jan 2011

Effect Of Elemental Sulfur Application On Ammonia Volatilization From Surface Applied Urea Fertilizer To Calcareous Sandy Soils, A.A. Soaud, M.E. Saleh, K.A. El-Tarabily, Mohd Sofian-Azirun, M.M. Rahman

Research Publications (2011 to 2015)

Widespread acceptance of urea was delayed in part due to its greater potential for nitrogen (N) loss via ammonia volatilization. Elemental sulfur (S(0)) at rates of 0, 1, 5 and 10 t ha(-1) combined with or without urea and inoculation of Paracoccus versutus (Pv) were tested to determine NH(3)-N volatilization from urea in sandy calcareous soils of Masafi-1 and Masafi-2. Daily NH(3)-N loss was measured up to 29 days after surface urea application in both soils using the closed dynamic airflow system. Total NH(3)-N volatilizations from urea-treated soils were 22.15 to 29.74 of applied N in Masafi -1 and Masafi …

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Effect Of Picloram, Additives And Plant Growth Regulators On Somatic Embryogenesis Of Phyla Nodiflora (L.) Greene, A.B.A. Ahmed, A.S. Rao, M.V. Rao, R.M. Taha Jan 2011

Effect Of Picloram, Additives And Plant Growth Regulators On Somatic Embryogenesis Of Phyla Nodiflora (L.) Greene, A.B.A. Ahmed, A.S. Rao, M.V. Rao, R.M. Taha

Research Publications (2011 to 2015)

The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid) markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture, which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10 ml/L), citric acid …

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Effects Of Different Pretreatment Methods On Anaerobic Mixed Microflora For Hydrogen Production And Cod Reduction From Palm Oil Mill Effluent, P. Mohammadi, Shaliza Ibrahim, M.S.M. Annuar, S. Law Jan 2011

Effects Of Different Pretreatment Methods On Anaerobic Mixed Microflora For Hydrogen Production And Cod Reduction From Palm Oil Mill Effluent, P. Mohammadi, Shaliza Ibrahim, M.S.M. Annuar, S. Law

Research Publications (2011 to 2015)

Effects of different pretreatment methods on sludge inocula were evaluated with respect to hydrogen (H 2) production enhancement using palm oil mill effluent (POME) in a batch system. The experimental results showed that the pretreatment methods (chemical, acid, heat-shock, freezing and thawing, and base) at 35 °C and initial pH 5.5 had a positive influence on H 2 production yield and chemical oxygen demand (COD) removal efficiency during the fermentative H 2 production as compared to the control experiments (without pretreatment). Heat-shock pretreatment method was shown to be a simple and useful method for enhancing both H 2 producing and …

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Effects Of Elemental Sulfur, Phosphorus, Micronutrients And Paracoccus Versutus On Nutrient Availability Of Calcareous Soils, A.A. Soaud, F.H. Al Darwish, M.E. Saleh, K.A. El-Tarabily, Mohd Sofian-Azirun, M.M. Rahman Jan 2011

Effects Of Elemental Sulfur, Phosphorus, Micronutrients And Paracoccus Versutus On Nutrient Availability Of Calcareous Soils, A.A. Soaud, F.H. Al Darwish, M.E. Saleh, K.A. El-Tarabily, Mohd Sofian-Azirun, M.M. Rahman

Research Publications (2011 to 2015)

This study was carried out in the laboratory to investigate the effects of elemental sulfur (S-0), Paracoccus versutus (Pv), phosphorus (P) and micronutrients (DTPA extractable Fe+Mn+Zn) both singly and combined on nutrient availability of calcareous soils. Soils were collected from Al Semaih, Al Dhahrah and Melaiha in United Arab Emirates (UAE) and all soils were incubated at 40 +/- 2(0)C for 32, 64, 96 and 128 days. Soil pH dropped and S concentration increased significantly with the addition of S-0 alone or in combination with Pv, P and micronutrients in all types of soils. Elemental S application considerably increased the …

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Efficient Propagation Of An Important Medicinal Plant Boesenbergia Rotunda By Shoot Derived Callus, N.A. Yusuf, M.S.M. Annuar, N. Khalid Jan 2011

Efficient Propagation Of An Important Medicinal Plant Boesenbergia Rotunda By Shoot Derived Callus, N.A. Yusuf, M.S.M. Annuar, N. Khalid

Research Publications (2011 to 2015)

A protocol for high frequency shoot organogenesis and plant establishment from shoot base derived callus was developed for Boesenbergia rotunda (L.) Mansf. Kulturpfl. The cultures produced callus in MS medium supplemented with various concentrations of dicamba and 2,4-D. Nevertheless, embryogenic callus only emerged on 2, 4-D augmented MS medium. In addition, the success of callus induction was achieved from meristem derived callus producing 100 of embryogenic callus. Multiple shoot induction was achieved from the surface of the callus after transferring onto MS medium supplemented with BAP or kinetin (shoot induction medium). The highest frequency of callus producing shoots (49.8) was …

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Enhancement In Mineralization Of A Number Of Natural Refractory Organic Compounds By The Combined Process Of Sonolysis And Ozonolysis (Us/O3), M. Cui, M. Jang, S.H. Cho, D. Elena, J. Khim Jan 2011

Enhancement In Mineralization Of A Number Of Natural Refractory Organic Compounds By The Combined Process Of Sonolysis And Ozonolysis (Us/O3), M. Cui, M. Jang, S.H. Cho, D. Elena, J. Khim

Research Publications (2011 to 2015)

As an advanced oxidation process, the combination of sonolysis (US)/ozonolysis (O3) was investigated on the treatment of tannic acid (TA) and humic acid (HA). In this study, biodegradable chemicals were found by the molecular weight and GC-MS analysis method, and mineralization rate and synergetic effects were also studied. For the water samples prior to the treatment of US/O3, ratios of molecular size higher than 5000 and 2000 Da for HA and TA, detected by the ultra filtration method, were 90.25 and 89.53, respectively. However, after 0.5 h of reacting, this ratio rapidly reduced to 3 and 4, and the ratios …

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Enzymatic And Toxinological Activities Of Hypnale Hypnale (Hump-Nosed Pit Viper) Venom And Its Fractionation By Ion Exchange High Performance Liquid Chromatography, C.H. Tan, S.M. Sim, C.A. Gnanathasan, Shin Yee Fung, G. Ponnudurai, J. Pailoor, N.H. Tan Jan 2011

Enzymatic And Toxinological Activities Of Hypnale Hypnale (Hump-Nosed Pit Viper) Venom And Its Fractionation By Ion Exchange High Performance Liquid Chromatography, C.H. Tan, S.M. Sim, C.A. Gnanathasan, Shin Yee Fung, G. Ponnudurai, J. Pailoor, N.H. Tan

Research Publications (2011 to 2015)

Hypnale hypnale (hump-nosed pit viper) has been recently identified as one of the medically important venomous snakes in Sri Lanka and on the southwestern coast of India. The characterization of its venom is essential for understanding the pathophysiology of envenomation and for optimizing its management. In the present study, the biological properties of Hypnale hypnale venom and venom fractions obtained using Resource Q ion exchange chromatography were determined. The venom exhibited toxic activities typical of pit viper venom, comparable to that of its sister taxon, the Malayan pit viper (Calloselasma rhodostoma). Particularly noteworthy were its high activities of thrombin-like enzyme, …

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Enzyme-Mediated Production Of Sugars From Sago Starch: Statistical Process Optimization, L.L. Wee, M.S.M. Annuar, Shaliza Ibrahim, Y. Chisti Jan 2011

Enzyme-Mediated Production Of Sugars From Sago Starch: Statistical Process Optimization, L.L. Wee, M.S.M. Annuar, Shaliza Ibrahim, Y. Chisti

Research Publications (2011 to 2015)

Glucoamylase (γ-amylase, EC 3.2.1.3) from Aspergillus niger was used to hydrolyze the soluble sago starch to reducing sugars without any major pretreatment of the substrate. A 2 L stirred tank reactor was used for the hydrolysis. The effects of pH, temperature, agitation speed, substrate concentration, and enzyme concentration on the reaction were investigated in order to maximize both the initial reaction velocity v and the final product yield Y p/s. A response surface methodology central composite design was used for the optimization. A maximum Y p/s of 0.58 g · g -1 and a high v of 0.50 mmoles ·L …

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Erectogenic Effects Of Clerodendron Capitatum: Involvement Of Phosphodiesterase Type-5 Inhibition, S.I. Abdelwahab, A.H. Mohamed, M. Oall, O.Y. Mohamed, M.M. Elhassan Taha, S. Mohan, M.I. Noordin, Mohd Rais Mustafa, K. M. Alkharfy Jan 2011

Erectogenic Effects Of Clerodendron Capitatum: Involvement Of Phosphodiesterase Type-5 Inhibition, S.I. Abdelwahab, A.H. Mohamed, M. Oall, O.Y. Mohamed, M.M. Elhassan Taha, S. Mohan, M.I. Noordin, Mohd Rais Mustafa, K. M. Alkharfy

Research Publications (2011 to 2015)

Clerodendron capitatum (Willd) (family: verbenaceae) is locally named as Gung and used traditionally to treat erectile dysfunction.Therefore, the current study was designed to investigate the erectogenic properties of C. capitatum. The relaxation effect of this plant was tested on phenylephrine precontracted rabbit corpus cavernosum smooth muscle (CCSM). The effects of C. capitatum were also examined on isolated Guinea pig atria alone, in the presence of calcium chloride (Ca 2+ channel blocker), atropine(cholinergic blocker), and glibenclamide (ATP-sensitive K +channel blocker). These effects were confirmed on isolated rabbit aortic strips. The extract, when tested colorimetrically for its inhibitory activities on phosphordiesterase-5 (PDE-5) …

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Establishment Of Avicennia Marina Mangroves On Accreting Coastline At Sungai Haji Dorani, Selangor, Malaysia, N.M. Tamin, R. Zakaria, Roslan Hashim, Y. Yin Jan 2011

Establishment Of Avicennia Marina Mangroves On Accreting Coastline At Sungai Haji Dorani, Selangor, Malaysia, N.M. Tamin, R. Zakaria, Roslan Hashim, Y. Yin

Research Publications (2011 to 2015)

Avicennia marina saplings were established within a restoration area at Sg. Hj. Dorani, Malaysia between May and August 2010. Sapling establishment was achieved following the construction of segmented and hard breakwater as mitigating measure against coastal erosion. Geostructures known as brush faschines were installed within the restoration area as secondary sediment and seed traps. There was a steady increase in the sand content of the soil from 5% in November 2008 to 18% in May 2010. The dominant soil content was silt but it fluctuated and declined slightly over the same period, between 80% in November 2008 and 72% in …

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Evaluation Of Methods For Estimating Fractal Dimension In Motor Imagery-Based Brain Computer Interface, C.K. Loo, A. Samraj, G.C. Lee Jan 2011

Evaluation Of Methods For Estimating Fractal Dimension In Motor Imagery-Based Brain Computer Interface, C.K. Loo, A. Samraj, G.C. Lee

Research Publications (2011 to 2015)

A brain computer interface BCI enables direct communication between a brain and a computer translating brain activity into computer commands using preprocessing, feature extraction, and classification operations. Feature extraction is crucial, as it has a substantial effect on the classification accuracy and speed. While fractal dimension has been successfully used in various domains to characterize data exhibiting fractal properties, its usage in motor imagery-based BCI has been more recent. In this study, commonly used fractal dimension estimation methods to characterize time series Katz's method, Higuchi's method, rescaled range method, and Renyi's entropy were evaluated for feature extraction in motor imagery-based …

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Evaluation Of The Dengue Ns1 Ag Stripâ® For Detection Of Dengue Virus Antigen In Aedes Aegypti (Diptera: Culicidae), C.H. Tan, P.S.J. Wong, M.Z.I. Li, I. Vythilingam, L.C. Ng Jan 2011

Evaluation Of The Dengue Ns1 Ag Stripâ® For Detection Of Dengue Virus Antigen In Aedes Aegypti (Diptera: Culicidae), C.H. Tan, P.S.J. Wong, M.Z.I. Li, I. Vythilingam, L.C. Ng

Research Publications (2011 to 2015)

Abstract Dengue fever is currently one of the most important mosquito-borne diseases that affect humans. With neither vaccines nor treatment available, prevention of the disease relies heavily on surveillance and control of mosquito vectors. In the present study, we have evaluated and showed the potential use of the Dengue NS1 Ag Strip® for the detection of dengue virus (DENV) in Aedes aegypti. Initial results showed that the sensitivity of the test kit in detecting DENV in wild-caught mosquitoes is comparable to that of real-time reverse transcriptaseâ��polymerase chain reaction. The detection of naturally infected Ae. aegypti with the NS1 rapid test …

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Expression And Analysis Of The Glycosylation Properties Of Recombinant Human Erythropoietin Expressed In Pichia Pastoris, Ser Huy Teh, Mun Yik Fong, Zulqarnain Mohamed Jan 2011

Expression And Analysis Of The Glycosylation Properties Of Recombinant Human Erythropoietin Expressed In Pichia Pastoris, Ser Huy Teh, Mun Yik Fong, Zulqarnain Mohamed

Research Publications (2011 to 2015)

The Pichia pastoris expression system was used to produce recombinant human erythropoietin, a protein synthesized by the adult kidney and responsible for the regulation of red blood cell production. The entire recombinant human erythropoietin (rhEPO) gene was constructed using the Splicing by Overlap Extension by PCR (SOE-PCR) technique, cloned and expressed through the secretory pathway of the Pichia expression system. Recombinant erythropoietin was successfully expressed in P. pastoris. The estimated molecular mass of the expressed protein ranged from 32 kDa to 75 kDa, with the variation in size being attributed to the presence of rhEPO glycosylation analogs. A crude functional …

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Extraction, Isolation And Characterization Of Bioactive Compounds From Plants' Extracts, S. Sasidharan, Y. Chen, D. Saravanan, K.M. Sundram, L.Y. Latha Jan 2011

Extraction, Isolation And Characterization Of Bioactive Compounds From Plants' Extracts, S. Sasidharan, Y. Chen, D. Saravanan, K.M. Sundram, L.Y. Latha

Research Publications (2011 to 2015)

Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations. The common problems and key challenges in …

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Fabrication And Characterization Of Graphene Hydrogel Via Hydrothermal Approach As A Scaffold For Preliminary Study Of Cell Growth, H. Lim, N. Huang, S. Lim, I. Harrison, C. Chia Jan 2011

Fabrication And Characterization Of Graphene Hydrogel Via Hydrothermal Approach As A Scaffold For Preliminary Study Of Cell Growth, H. Lim, N. Huang, S. Lim, I. Harrison, C. Chia

Research Publications (2011 to 2015)

BACKGROUND: Three-dimensional assembly of graphene hydrogel is rapidly attracting the interest of researchers because of its wide range of applications in energy storage, electronics, electrochemistry, and waste water treatment. Information on the use of graphene hydrogel for biological purposes is lacking, so we conducted a preliminary study to determine the suitability of graphene hydrogel as a substrate for cell growth, which could potentially be used as building blocks for biomolecules and tissue engineering applications. METHODS: A three-dimensional structure of graphene hydrogel was prepared via a simple hydrothermal method using two-dimensional large-area graphene oxide nanosheets as a precursor. RESULTS: The concentration …

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