Digital Commons Network^™

Effect Of Benzylaminopurine (Bap) Pulsing On In Vitro Shoot Multiplication Of Musa Acuminata (Banana) Cv. Berangan, N. Jafari, R.Y. Othman, Norzulaani Khalid

Research Publications (2011 to 2015)

MS (Murashige and Skoog) media supplemented with benzylaminopurine (BAP) showed that the number of bud formation in shoot cultures of Musa acuminata cv.Berangan during the initiation stage increased proportionately with the concentrations used (11, 22 and 33 μM). However, the highest concentration of BAP (33 μM) simultaneously increased the formation of abnormal shoots. After the first apical bud appeared, explants were transferred to MS medium with lower concentrations of BAP either with or without indole acetic acid (IAA). Proliferation media supplemented with IAA showed enhanced shoot multiplication and elongation but did not help to reduce the abnormality index that occurred

Effects Of Sulfur And Nitrogen On Nutrients Uptake Of Corn Using Acidified Water, M. Motior Rahman, A.A. Soaud, F.H. Al Darwish, Mohd Sofian-Azirun

Research Publications (2011 to 2015)

A greenhouse experiment was carried out with elemental sulfur (S) and nitrogen (N) fertilizer using acidified water (pH 6.5) to determine nutrients uptake and growth of maize plants grown in calcareous sandy soil. Four levels of elemental sulfur (0, 1, 5 and 10 t ha -1), two levels of N (0 and 0.34 t N ha -1) were tested at Al Foah Agricultural Experiment Farm, United Arab Emirates (UAE) University. In Al Semaih soil, the pH (1.21-1.33) and electrical conductivity (EC) (7.61 dSm -1) decreased by application of elemental S plus N, while EC rose (3.84 dSm -1) and pH …

Evaluation Of N ε-(Carboxymethyl)Lysine And Lipid Peroxidation In Multiethnic Malaysian Subjects With Type 2 Diabetes Mellitus, K.A.A. Ahmed, S. Muniandy, I.S. Ismail, R.S. Ali, Z.H. Alhamodi

Research Publications (2011 to 2015)

The glycoxidation product, N ε-(carboxymethyl)lysine (CML), is formed during reaction of glucose with protein under oxidative conditions. The aim of the study was to evaluate the levels of the most common glycoxidation product; N ε-(carboxymethyl)lysine (CML) and the most advanced lipoxidation product, malondialdehyde (MDA) in type 2 diabetic Malay, Indian, and Chinese ethnic groups in the Malaysian population. Studies were performed on age-matched Malay, Indian, and Chinese subjects with type 2 diabetes mellitus. Different biochemical parameters including serum levels of CML, MDA, lipid profile, glucose, and hemoglobin A1c (HbA 1c) were measured by standard methods. The relationships between circulating CML …

Fabrication And Characterization Of Graphene Hydrogel Via Hydrothermal Approach As A Scaffold For Preliminary Study Of Cell Growth, H.N. Lim, N.M. Huang, S. Lim, I. Harrison, C.H. Chia

Research Publications (2011 to 2015)

Three-dimensional assembly of graphene hydrogel is rapidly attracting the interest of researchers because of its wide range of applications in energy storage, electronics, electrochemistry, and waste water treatment. Information on the use of graphene hydrogel for biological purposes is lacking, so we conducted a preliminary study to determine the suitability of graphene hydrogel as a substrate for cell growth, which could potentially be used as building blocks for biomolecules and tissue engineering applications. A three-dimensional structure of graphene hydrogel was prepared via a simple hydrothermal method using two-dimensional large-area graphene oxide nanosheets as a precursor. The concentration and lateral size …

Hepatoprotective Effects Of Orthosiphon Stamineus Extract On Thioacetamide-Induced Liver Cirrhosis In Rats, Mohammed A. Alshawsh, Mahmood Ameen Abdulla, Salmah Ismail, Zahra A. Amin

Research Publications (2011 to 2015)

Orthosiphon stamineus as medicinal plant is commonly used in Malaysia for treatment of hepatitis and jaundice; in this study, the ethanol extracts were applied to evaluate the hepatoprotective effects in a thioacetamide-induced hepatotoxic model in Sprague Dawley rats. Five groups of adult rats were arranged as follows:Group 1 (normal control group), Group 2 Thioacetamide (TAA) as positive control (hepatotoxic group), Group 3 Silymarin as a well-known standard drug (hepatoprotective group), and Groups 4 and 5 as high and low dose (treatment groups). After 60-day treatment, all rats were sacrificed. The hepatotoxic group showed a coarse granulation on the liver surface …

Mucuna Pruriens Linn. Seed Extract Pretreatment Protects Against Cardiorespiratory And Neuromuscular Depressant Effects Of Naja Sputatrix (Javan Spitting Cobra) Venom In Rats, S.Y. Fung, N.H. Tan, S.M. Sim, E. Marinello, R. Guerranti

Research Publications (2011 to 2015)

Mucuna pruriens has been used by native Nigerians as a prophylactic for snakebite. The protective effects of M. pruriens seed extract (MPE) were investigated against the pharmacological actions of N. sputatrix (Javan spitting cobra) venom in rats. The results showed that MPE-pretreatment protected against cardiorespiratory and, to a lesser extent, neuromuscular depressant effects of N. sputatrix venom. These may be explained at least in part by the neutralisation of the cobra venom toxins by anti-MPE antibodies elicited by the MPE pretreatment.

The Application Of Melatonin And Platelet-Rich Plasma In The Development Of A Bioactive Calcium Aluminate Bone Regenerative Scaffold, William Clafshenkel

Electronic Theses and Dissertations

Over 500,000 bone graft procedures are conducted annually within the United States. Autografts contribute to donor site complications and disease transmission with allografts has been described. Many ceramics are only osteoconductive and are brittle, limiting their clinical use. Thus, the objective of this study was to create a bone substitute with osteoinductive properties similar to natural bone using the ceramic biomaterial calcium aluminate (CA). Calcium aluminate materials are durable and remain moldable for an extended period of time at room temperature. Further, the surfaces of CA scaffolds can be modified with biological agents through simple chemical means to locally deliver …

A Phenomenology Of Occupation-Based Hand Therapy, Donna Colaianni

Electronic Theses and Dissertations

The founding philosophies of occupational therapy and the medical model have been in conflict within the practice of occupational therapy for more than 60 years. This conflict is especially evident in the hand therapy arena due to the prevalence of the medical model. There is extensive literature outside this specialty area indicating that an occupation-based approach is beneficial for patients with whom it is used yet there is a lack of research on occupation-based hand therapy. Clearly describing and defining what constitutes occupation-based hand therapy can help promote occupation-based practice in keeping with the philosophy of the profession and facilitate …

The Domestic Church And The Post-Nuclear Family: A Theological Analysis And Critique, Corey Harris

Electronic Theses and Dissertations

This dissertation is an attempt to reconcile the Roman Catholic theological understanding that the Christian family is a domestic church (ecclesia domestica) with a realistic demographic and sociological portrait of the American family while remaining faithful to Catholic moral teaching. The first chapter analyzes several works of the Magisterium from the time of Vatican II that have specifically referenced the domestic church. These documents collectively show that the domestic church is a sacramental and ecclesial reality although the theology is commonly espoused under the subheading of marriage and referencing a specifically nuclear form of family. The following chapter analyzes the …

Roles Of Carboxyl Groups In The Transmembrane Insertion Of Peptides, Francisco N. Barrera, Dhammika Weerakkody, Michael Anderson, Oleg A. Andreev, Yana K. Reshetnyak, Donald M. Engelman

Physics Faculty Publications

We have used pHLIP® [pH (low) insertion peptide] to study the roles of carboxyl groups in transmembrane (TM) peptide insertion. pHLIP binds to the surface of a lipid bilayer as a disordered peptide at neutral pH; when the pH is lowered, it inserts across the membrane to form a TM helix. Peptide insertion is reversed when the pH is raised above the characteristic pK_a (6.0). A key event that facilitates membrane insertion is the protonation of aspartic acid (Asp) and/or glutamic acid (Glu) residues, since their negatively charged side chains hinder membrane insertion at …

Tuning A Polar Molecule For Selective Cytoplasmic Delivery By A Ph (Low) Insertion Peptide, Dayanjali Wijesinghe, Donald M. Engelman, Oleg A. Andreev, Yana K. Reshetnyak

Physics Faculty Publications

Drug molecules are typically hydrophobic and small in order to traverse membranes to reach cytoplasmic targets, but we have discovered that more polar molecules can be delivered across membranes using water-soluble, moderately hydrophobic membrane peptides of the pHLIP (pH low insertion peptide) family. Delivery of polar cargo molecules could expand the chemical landscape for pharmacological agents that have useful activity but are too polar by normal drug criteria. The spontaneous insertion and folding of the pHLIP peptide across a lipid bilayer seeks a free energy minimum, and insertion is accompanied by a release of energy that can be used to …

Measuring Tumor Aggressiveness And Targeting Metastatic Lesions With Fluorescent Phlip, Yana K. Reshetnyak, Lan Yao, Sida Zheng, Sergey Kuznetsov, Donald M. Engelman, Oleg A. Andreev

Physics Faculty Publications

Purpose: Malignant cancer foci develop acidic extracellular environments. Mild acidic conditions trigger insertion and folding of the pH (low) insertion peptide (pHLIP^TM) across a cellular membrane, enabling targeting of such lesions.

Procedures: We employed optical imaging to follow targeting by fluorescent pHLIP given i.v. in mice. For visualization, Cy5.5 and Alexa750 were covalently attached to the N terminus of pHLIP, which stays outside of a cell membrane after transmembrane insertion.

Results: We demonstrate that pHLIP targets: (a) tumors of different origins established by subcutaneous injection of cancer cells, (b) spontaneous prostate tumors in TRAMP mice and (c) metastatic …

Rollins Magazine, Fall 2011, Rollins College Office Of Marketing And Communications

Rollins Magazine

Issue Title: "High-Impact Learning"

Annual Report, Mississippi State University Foundation, Amy Cagle

MSUF Annual Reports

This document is the annual report for Mississippi State University Foundation.

Common Data Set, Office Of Institutional Research And Effectiveness, Mississippi State University

Common data set

No abstract provided.

Mississippi State University - Student Enrollment Profile - Fall 2011, Mississippi State University Office Of Institutional Research And Effectiveness

Enrollment Profiles

No abstract provided.

Occurrence Of Blastocystis In Water Of Two Rivers From Recreational Areas In Malaysia, I. Ithoi, A. Jali, J.W. Mak, W.Y. Wan Sulaiman, R. Mahmud

Research Publications (2011 to 2015)

This study reports the occurrence of Blastocystis in water from two rivers, Sungai Congkak and Sungai Batu, located in recreational areas in Malaysia. This protozoan was detected in samples from both rivers with an average of 33.3% and 22.1%, respectively. It was detected highest at the downstream (73.8% and 33.8%) followed by midstream (17.5% and 25.0%) and upstream (8.8% and 7.5%) stations, with additionally higher detection during holidays (with average 47.5% and 30.8%) than week days (with average 19.2% and 13.3%), in both rivers, respectively. There was a strong association with the daily activities of locals and visitors, who came …

Panduratin A Inhibits The Growth Of A549 Cells Through Induction Of Apoptosis And Inhibition Of Nf-Kappab Translocation, S.C. Cheah, D.R. Appleton, S.T. Lee, M.L. Lam, A.H.A. Hadi, Mohd Rais Mustafa

Research Publications (2011 to 2015)

In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC50 value of 4.4 µg/mL 10.8 µM). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as …

Postharvest Physico-Chemical And Mechanical Changes In Jambu Air (Syzygium Aqueum Alston) Fruits, M. Tehrani, S. Chandran, A.B.M.S. Hossain, A. Nasrulhaq-Boyce

Research Publications (2011 to 2015)

Little data is available in the scientific literature on postharvest changes taking place in Syzygium aqueum fruits, an increasingly popular fruit in the Asian region. In this study the postharvest physico-chemical and mechanical properties, namely, fruit color, weight loss, pulp firmness, total soluble solids, pH and titratable acidity were determined during the postharvest storage period under ambient conditions of Syzygium aqueum fruits. It was observed that weight loss, total soluble solids (TSS) and pH of the jambu air fruits increased with time whilst pulp firmness and the color index of the fruits decreased. Analysis of the antioxidant activity, determined in …

Potential Application Of Sludge Produced From Coal Mine Drainage Treatment For Removing Zn(Ii) In An Aqueous Phase, M. Cui, M. Jang, S.H. Cho, J. Khim

Research Publications (2011 to 2015)

Various analyses of physico-chemical characteristics and batch tests were conducted with the sludge obtained from a full-scale electrolysis facility for treating coal mine drainage in order to find the applicability of sludge as a material for removing Zn(II) in an aqueous phase. The physico-chemical analysis results indicated that coal mine drainage sludge (CMDS) had a high specific surface area and also satisfied the standard of toxicity characteristic leaching procedure (TCLP) because the extracted concentrations of certain toxic elements such as Pb, Cu, As, Hg, Zn, and Ni were much less than their regulatory limits. The results of X-ray diffraction (XRD) …

Profiling Of Burkholderia Cepacia Secretome At Mid-Logarithmic And Early-Stationary Phases Of Growth, Vanitha Mariappan, Kumutha Malar Vellasamy, Onn Haji Hashim, Jamuna Vadivelu

Research Publications (2011 to 2015)

Background: Burkholderia cepacia is a Gram-negative pathogen that causes serious respiratory infections in immunocompromised patients and individuals with cystic fibrosis. This bacterium is known to release extracellular proteins that may be involved in virulence. Methodology/Principal Findings: In the present study, B. cepacia grown to mid-logarithmic and early-stationary phases were investigated on their ability to invade and survive intracellularly in A549 lung epithelial cells in order to discern the fate of these bacteria in the pathogenesis of B. cepacia lung infections in in vitro condition. The early-stationary phase B. cepacia was demonstrated to be more invasive than mid-logarithmic phase. In addition, …

Studies On Seed And Forage Yield In 10 Populations Of Sainfoin (Onobrychis Saliva) Grown As Spaced Plants And Swards, S. Mohajer, A.A. Jafari, R.M. Taha

Research Publications (2011 to 2015)

In order to study seed and dry matter (DM) yield and relationships between yield and morphological traits, 10 populations of sainfoin (Onobrychis saliva), were assessed in two separate experiments under sward and spaced plant conditions using complete block design with two replications, in Karaj, Iran, during 2004-2007. The data were collected for seed yield, DM yield, plant height, stem number per m(2), flowering date, inflorescences length and 1000-grain weight. The data were analyzed for individual experiment and combined over two experiments. Results showed significant differences among populations for all of traits except 1000-grain weight in sward and DM yield and …

Somatic Embryogenesis And Plant Regeneration From Hypocotyl And Leaf Explants Of Brassica Oleracea Var. Botrytis (Cauliflower), P.K. Siong, R.M. Taha, F.A. Rahiman

Research Publications (2011 to 2015)

We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis On MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages …

The Concept Of Two-Dimensional Electrophoresis-Guided Purification Proven By Isolation Of Rhodocetin From Calloselasma Rhodostoma (Malayan Pit Viper), M.S. Tang, J. Vejayan, H. Ibrahim

Research Publications (2011 to 2015)

Two-dimensional gel electrophoresis (2DE) is an important tool for investigating the complexity of snake venom proteomes. Apart from applications based on whole proteome analysis, we suggest that 2DE can be used as an assay to guide the progress of protein purification. The aim of this study was to prove the feasibility of this concept by using it to purify rhodocetin from Calloselasma rhodostoma venom. Rhodocetin (α subunit) spot on the 2DE profile of C. rhodostoma venom was first identified and confirmed by mass spectrometry, with a molecular mass of 16 kDa and calculated pI of 5.16. Rhodocetin was subsequently purified …

Ultrasonography In The Evaluation Of Carpal Tunnel Syndrome: Diagnostic Criteria And Comparison With Nerve Conduction Studies, K.Y. Chan, J. George, K.J. Goh, T.S. Ahmad

Research Publications (2011 to 2015)

Ultrasound criteria for carpal tunnel syndrome (CTS) may vary in different populations. To determine the ultrasonographic criteria for CTS in a Malaysian population and compare its usefulness with nerve conduction studies (NCS), we studied patients clinically diagnosed with CTS and normal controls by ultrasonography. All patients also underwent standard NCS. Median nerve Cross-Sectional Area (CSA) and Flattening Ratio (FR) at 3 different levels – proximal to tunnel inlet, at tunnel inlet and tunnel outlet were measured. Receiver operator characteristic (ROC) analyses were used to calculate the optimal discriminatory threshold values for CTS. Of 54 CTS hands, NCS was positive in …

Antimicrobial Susceptibility Profiling And Genomic Diversity Of Multidrug-Resistant Acinetobacter Baumannii Isolates From A Teaching Hospital In Malaysia, Boon Hong Kong, Yasmin Abu Hanifah, Mohd Yasim Mohd Yusof, Kwai Lin Thong

Research Publications (2011 to 2015)

The resistance phenotypes and genomic diversity of 185 Acinetobacter baumannii isolates obtained from the intensive care unit (ICU) of a local teaching hospital in Kuala Lumpur from 2006 to 2009 were determined using antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE). Antibiogram analyses showed that the isolates were fully resistant to β-lactam antimicrobials and had high resistance rates to the other antimicrobial agents tested. However, the isolates were susceptible to polymyxin B. Resistance to cefoperazone/sulbactam was only detected in strains isolated from 2007 to 2009. Some environmental isolates and an isolate from the hands of a healthcare worker (HCW) had …

Application Of Artificial Neural Network On Vibration Test Data For Damage Identification In Bridge Girder, S.J.S. Hakim, H. Abdul Razak

Research Publications (2011 to 2015)

Structures are exposed to damage during their service life which can severely affect their safety and functionality. Thus, it is important to monitor structures for the occurrence, location and extent of damage. Artificial neural networks (ANNs) as a numerical technique have been applied increasingly for damage identification with varied success. ANNs are inspired by human biological neurons and have been used to model some specific problems in many areas of engineering and science to achieve reasonable results. ANNs have the ability to learn from examples and then adapt to changing situations when sufficient input-output data are available. This paper presents …

Massive Modularity, Content Integration, And Language, Collin Rice

Philosophy Faculty Research and Scholarship

One of the obstacles facing massive modularity is how a pervasively modular mind might generate non-domain-specific thoughts by integrating the content produced by various domain-specific modules. Peter Carruthers has recently argued that the operations of the language faculty are constitutive of the process by which the human mind is able to integrate content from heterogeneous conceptual domains. In this article, I first argue that Carruthers’s data do not provide support for either of two possible interpretations of his thesis. In addition, I provide empirical and theoretical reasons for thinking content integration is performed external to the language faculty.

Culture And Passionate Love, Elaine Hatfield, Richard L. Rapson

Papers from the International Association for Cross-Cultural Psychology Conferences

For more than 4,000 years, poets and storytellers have sung of the delights and sufferings of love and lust. This chapter reviews what scholars from various disciplines have discovered about the nature of passionate love and sexual desire. Anthropologists and evolutionary psychologists have assumed that passionate love is a cultural universal. Cultural researchers, historians, and social psychologists have emphasized the stunning diversity in the way passionate love and sexual desire have been viewed and experienced. Culture, ethnicity and the rules passed down by political and religious authorities have a profound impact on the way people think about and act out …

Can Happiness Change? An Interdisciplinary, Multi-Method Investigation Of The Dynamics Of Happiness, Franziska Deutsch, Mandy Boehnke, Ulrich Kühnen, Klaus Boehnke

Papers from the International Association for Cross-Cultural Psychology Conferences

None of the major basic questions social sciences are concerned with can satisfyingly be answered from the perspective of one discipline alone. Each of them proposes theories and perspectives that make unique and important contributions. At the same time theoretical perspectives in general inevitably do have their blind spots. This fundamental insight was the reason for us to choose as the motto for the 19th IACCP congress held in Bremen in 2008 “Crossing borders – (Cross-) Cultural Psychology as an Interdisciplinary, Multi-Method Endeavor”. In this chapter we first want to illustrate this motto and our reasons for choosing it by …