Digital Commons Network^™

The Uninvited, Tim Kelly

SFA School of Theatre

No abstract provided.

School House Rock Live!, Scott Shattuck

SFA School of Theatre

No abstract provided.

Microrna Profiling Reveals Age-Dependent Differential Expression Of Nuclear Factor B And Mitogen-Activated Protein Kinase In Adipose And Bone Marrow-Derived Human Mesenchymal Stem Cells, Amitabh C. Pandey, Julie A. Semon, Deepak Kaushal, Regina P. O'Sullivan, Julie Glowacki, Jeffrey M.. Gimble, Bruce A. Bunnell

Biological Sciences Faculty Research & Creative Works

Introduction. Mesenchymal stem cells (MSCs) play a central role in mediating endogenous repair of cell and tissue damage. Biologic aging is a universal process that results in changes at the cellular and molecular levels. In the present study, the role of microRNA (miRNA) in age-induced molecular changes in MSCs derived from adipose tissue (ASCs) and bone marrow (BMSCs) from young and old human donors were investigated by using an unbiased genome-wide approach.

Methods. Human ASCs and BMSCs from young and old donors were cultured, and total RNA was isolated. The miRNA fraction was enriched and used to determine the expression …

Casein Α S1 Is Expressed By Human Monocytes And Upregulates The Production Of Gm-Csf Via P38 Mapk, Stefan Vordenbäumen, Achim Braukmann, Klaudia Petermann, Andrea Scharf, Ellen Bleck, Anna Von Mikecz, Joachim Jose, Matthias Schneider

Biological Sciences Faculty Research & Creative Works

Caseins are major constituents of mammalian milks that are thought to be exclusively expressed in mammary glands and to function primarily as a protein source, as well as to ameliorate intestinal calcium uptake. In addition, proinflammatory and immunomodulatory properties have been reported for bovine caseins. Our aim was to investigate whether human casein α s1 (CSN1S1) is expressed outside the mammary gland and possesses immunomodulatory functions in humans as well. For this purpose, CSN1S1 mRNA was detected in primary human monocytes and CD4+ and CD8+ T cells, but not in CD19 + B cells. CSN1S1 protein was traceable in supernatants …

The “Definitiveness” Of Genocide And A Question Of Genocide: A Review Essay, Bedross Der Matossian

Department of History: Faculty Publications

Over the course of the past two decades, the historiography of the Armenian Genocide has evolved through the introduction of new methodologies, approaches, and more complex analyses of the Genocide that venture beyond rudimentary and essentialist arguments and representations. Concomitantly, denialist literature has also developed, reinvigorated in the U.S. by the presentation of alternative ways of viewing the event in order to counter “Armenian allegations.” The latest such endeavor, disguised under the cloak of “scholarship,” has been the introduction of the concept of “crimes against humanity” as an alternative designation to genocide or as a new “compromise” when dealing with …

Csr 2.0: Transforming The Role Of Business In Society, Wayne Visser

Social Space

Corporate Social Responsibility (or CSR) comes in different forms and shades. But according to Wayne Visser, it also comes in different stages, most of which have failed. Save, perhaps, for one.

Effect Of Feeding Wet Distillers Grains With Solubles To Beef Cattle On Air And Manure Quality, M. J. Spiehs, D. N. Miller, B. L. Woodbury, R. A. Eigenberg, V. H. Varel, D. B. Parker

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Air quality is becoming a pressing issue for beef feedlot producers. Feeding practices influence the excretion of starch, fiber, nitrogen (N), and sulfur (S) in manure, thereby affecting nutrient content and the production of ammonia (NH₃) and odorous compounds. Wet distillers grains with solubles (WDGS) are a common and economical ingredient in feedlot diets. WDGS are high in protein, fiber, phosphorus (P), and S but low in starch. The objective of this study was to compare NH3 concentration in the air and nutrients and volatile organic compounds (VOC) concentration in manure between two dietary treatments fed to feedlot …

Systematics And Biogeography Of The Clusioid Clade (Malpighiales), Brad R. Ruhfel

Biological Sciences Faculty and Staff Research

The clusioids are a clade of flowering plants in the diverse rosid order Malpighiales. It includes five families (i.e., Bonnetiaceae, Calophyllaceae, Clusiaceae sensu stricto, Hypericaceae, and Podostemaceae) that form a conspicuous element of tropical forests worldwide and are economically important. Their phylogenetic and biogeographical history has remained uncertain, however, which has hindered our understanding of their evolution. I conducted the first taxon-rich multigene analysis of this important clade to clarify their phylogenetic relationships (Chapter 1). Plastid (cp:matK, ndhF, and rbcL) and mitochondrial (mt: matR) nucleotide sequence data from nearly 200 taxa produced a well-resolved clusioid phylogeny and indicate that several …

Novel Copper Loaded Core-Shell Silica Nanoparticles With Improved Copper Bioavailability Synthesis, Characterization And Study Of Antibacterial Properties, Pavithra Maniprasad

Electronic Theses and Dissertations

A novel core-shell silica based antimicrobial nanoparticle was synthesized. The Stöber silica shell has been engineered to accommodate copper. Synthesis of the core-shell Cu-silica nanoparticle (C-S CuSiNP) involves preparation of base-hydrolyzed Stöber silica “seed” particles first, followed by the acid-catalyzed seeded growth of the Cu-silica shell layer around the core. Scanning electron microscopy and transmission electron microscopy showed monodispersed, spherical shaped nanoparticles with smooth surface morphology. Characterization of particle size distribution in solution by the Dynamic Light Scattering (DLS) technique was fairly consistent with the electron microscopy results. Loading of Cu to nanoparticles was confirmed by the SEM-Energy Dispersive X-Ray …

A Solid Phase Assay For Topoisomerase I Interfacial Poisons And Catalytic Inhibitors, Vidusha Cyril

Electronic Theses and Dissertations

We report a mechanism based screening technique to rapidly identify eukaryotic topoisomerase I targeting agents. The method is based on genetic tagging of topoisomerase I to immobilize the enzyme on a solid surface in a microtiter well format. DNA is added to the wells and retained DNA is detected by Picogreen fluorescence. Compounds that result in an increase in Picogreen staining represent potential topoisomerase interfacial poisons while those that reduce fluorescence report catalytic inhibitors; therefore, the solid phase assay represents a „bimodal‟ readout that reveals mechanisms of action. The method has been demonstrated to work with known interfacial poisons and …

Co-Culture Of Yeast Antagonists Of Fusarium Head Blight And Their Effect On Disease Development In Wheat, D. A. Schisler, P. J. Slininger, Michael J. Boehm, P. A. Paul

Department of Plant Pathology: Faculty Publications

Multistrain mixtures of biocontrol agents which can reduce plant disease to a greater extent than the individual strains of the mixture, commonly, are prepared by blending separately produced fermentation products. Co-cultivation of strains to equivalent biomass yields would provide mixture advantages without incurring the cost disadvantages of multiple fermentation and processing protocols. Fusariwn Head Blight (FHB) antagonists Cryptococcus flavescens OR 182.9 (NRRL Y-302l6), C. aureus OH 181.1 (NRRL Y-302l5) and C. aureus OR 181.1 (NRRL Y -30215), were grown in two- and three-strain co-cultures to assess the quality and efficacy of the fermentation end products produced. Final cell counts of …

The Gc-Rich Mitochondrial And Plastid Genomes Of The Green Alga Coccomyxa Give Insight Into The Evolution Of Organelle Dna Nucleotide Landscape, David Roy Smith, Fabien Burki, Takashi Burki, Jane Grimwood, Igor V, Grigoriev, James L. Van Etten, Patrick J. Keeling

Department of Plant Pathology: Faculty Publications

Most of the available mitochondrial and plastid genome sequences are biased towards adenine and thymine (AT) over guanine and cytosine (GC). Examples of GC-rich organelle DNAs are limited to a small but eclectic list of species, including certain green algae. Here, to gain insight in the evolution of organelle nucleotide landscape, we present the GC-rich mitochondrial and plastid DNAs from the trebouxiophyte green alga Coccomyxa sp. C-169. We compare these sequences with other GC-rich organelle DNAs and argue that the forces biasing them towards G and C are nonadaptive and linked to the metabolic and/or life history features of this …

Pacs Bulletin 2011-2012, Program In Audiology And Communication Sciences

PACS News

No abstract provided.

Screening For Cytomegalovirus During Pregnancy, Stuart P. Adler

Pediatrics Publications

The epidemiology and pathogenesis of CMV infections among pregnant women have been intensely studied over the last three decades. This paper highlights recent developments that make either universal or limited serologic screening for CMV during pregnancy potentially attractive. The developments include an understanding of the pathogenesis of CMV infections, a knowledge of high-risk women, the availability of accurate methods for the serologic diagnosis of a primary CMV infection using either single or serial blood samples, accurate methods for the diagnosis of fetal infection via amniotic fluid, sensitive fetal and placental indicators for neonatal outcomes, and the availability of potentially effective …

Corticotrophin-Releasing Hormone Type 1 Receptor Gene (Crhr1) Variants Predict Posttraumatic Stress Disorder Onset And Course In Pediatric Injury Patients, Ananda B. Amstadler, Nicole R. Nugent, Bao-Zhu Yang, Alisa Miller, Richie Siburian, Priya Moorjani, Stephen Haddad, Aditi Basu, Jesen Fagerness, Glenn Saxe, Jordan W. Smoller, Karestan C. Koenen

Psychiatry Publications

Posttraumatic stress disorder (PTSD) is a common and disabling anxiety disorder that may occur in the aftermath of exposure to potentially traumatic life events. PTSD is moderately heritable, but few specific molecular variants accounting for this heritability have been identified. Genes regulating the hypothalamic-pituitary-adrenal (HPA) axis, such as corticotrophin-releasing hormone type 1 receptor gene (CRHR1), have been implicated in traumatic-stress related phenotypes but have yet to be studied in relation to PTSD. The present study sought to examine the relation between 9 single nucleotide polymorphisms (SNPs) in the CRHR1 gene and posttraumatic stress symptoms in a prospective study of pediatric …

Acsl6 Is Associated With The Number Of Cigarettes Smoked And Its Expression Is Altered By Chronic Nicotine Exposure, Jingchun Chen, Darlene H. Brunzell, Kia J. Jackson, Andrew Van Der Vaart, Jennie Z. Ma, Thomas J. Payne, Richard Sherva, Lindsay A. Farrer, Pablo Gejman, Douglas F. Levinson, Peter Holmans, Steven H. Aggen, Imad Damaj, Po-Hsiu Kuo, Bradley T. Webb, Raymond Anton, Henry R. Kranzler, Joel Gelemter, Minf D. Li, Kenneth S. Kendler, Xiangning Chen

Psychiatry Publications

Individuals with schizophrenia tend to be heavy smokers and are at high risk for tobacco dependence. However, the nature of the comorbidity is not entirely clear. We previously reported evidence for association of schizophrenia with SNPs and SNP haplotypes in a region of chromosome 5q containing the SPEC2, PDZ-GEF2 and ACSL6 genes. In this current study, analysis of the control subjects of the Molecular Genetics of Schizophrenia (MGS) sample showed similar pattern of association with number of cigarettes smoked per day (numCIG) for the same region. To further test if this locus is associated with tobacco smoking as measured …

Minimal Art: Or Why Small Viral K+ Channels Are Good Tools For Understanding Basic Structure And Function Relations, Gerhard Thiel, Dirk Baumeister, Indra Schroeder, Stefan M. Kast, James L. Van Etten, Anna Moroni

James Van Etten Publications

Some algal viruses contain genes that encode proteins with the hallmarks of K+ channels. One feature of these proteins is that they are less than 100 amino acids in size, which make them truly minimal for a K+ channel protein. That is, they consist of only the pore module present in more complex K+ channels. The combination of miniature size and the functional robustness of the viral K+ channels make them ideal model systems for studying how K+ channels work. Here we summarize recent structure/function correlates from these channels, which provide insight into functional properties such as gating, pharmacology and …

Performance Screening Of Chemostat Adapted Recombinant Zymomonas Mobilis Strains, Deirdre M. Beard, Nancy Dowe

STAR Program Research Presentations

Corn stover biomass can be pretreaed and hydrolyzed into soluble sugars to be fermented by microorganisms to ethanol. NREL has developed a recombinant bacteria Zymomonas mobilis 8b that metabolizes both five and six carbon sugars. During pretreatment, toxic inhibitors such as furfural and acetate are produced. NREL has made an attempt to adapt two sub-strains of Z. mobilis 8b to acetate and furfural by using a chemostat method. During the chemostat process, cultures were frozen back in glycerol and saved. In this study, those frozen cultures were revived and analyzed for performance in environments with varying concentrations of furfural and …

Verification Of The Kcat Value Of Aat Via Copasi, Jeremy A. Waddell, Roque Troz, Melissa P. Hill, Delmar S. Larsen

STAR Program Research Presentations

PLP is a chromophoric cofactor required for catalytic activity by a wide variety of enzymes. While PLP enzymes are thermally activated in vivo, it has been reported that some PLP enzymes can be activated by UV light. One enzyme that uses PLP as a cofactor is apsartate aminotransferase (AAT). Previous studies with AAT suggest that the carbanionic quinonoid intermediate is photogenerated by UV laser excitation. AAT is central to nitrogen metabolism in all living systems and has a large body of literature. As such, it is a useful prototype for the fundamental studies on this class of enzymes which …

Caffeine Metabolism And Cytochrome P450 Enzyme Mrna Expression Levels Of Genetically Diverse Inbred Mouse Strains, Neal Addicott, Michael Malfatti, Gabriela G. Loots

STAR Program Research Presentations

Cytochrome P450 (CYP) enzymes activate or eliminate medications and other xenobiotics, and Caffeine has been used as an indicator of activity for some CYPs. The natural genetic variation in the human population for caffeine metabolizing CYPs does not yet have an established animal model for comparison, but the natural variation in inbred mouse strains presents an attractive starting point. The project gave four different strains of mice a 40mg/kg dose of caffeine and sampled blood and liver tissue after 30 minutes. Samples were analyzed using High Performance Liquid Chromatography (HPLC), reverse transcription PCR (rtPCR), and quantitative PCR (qPCR). The DBA/2J …

Contrasting Effects Of Flow On Adult And Juvenile Ascidian Life History Stages, Including The Global Invasive Didemnum Vexillum, Tren C. Kauzer, C Sarah Cohen

STAR Program Research Presentations

Few studies have quantified a relationship between recruitment, growth, survival and flow with colonial filter feeders. In this study, the growth of D. vex and larval recruitment were evaluated under different local flow conditions in Half Moon Bay, California, USA. Tunicates were subjected to four different flow conditions, three of which included objects to differentially obstruct flow, and growth and recruitment were measured. A direct relationship was seen between increases in flow and adult growth, and an inverse relationship was seen between increases in flow and recruitment. These trends suggest that optimal flow conditions differ for juveniles and adults. The …

College Of Liberal Arts Magazine, Fall 2010/Winter 2011, College Of Liberal Arts

College of Liberal Arts Magazine

No abstract provided.

California Polytechnic State University 2011-2013 Catalog, Academic Affairs, California Polytechnic State University - San Luis Obispo

Course Catalogs

No abstract provided.

Annual Reports Of The Town Officers, Town Of Stoddard, New Hampshire For The Fiscal Year Ending June 30, 2011., Stoddard Town Representatives

Stoddard, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

A P38Α-Selective Chemosensor For Use In Unfractionated Cell Lysates, Cliff I. Stains, Elvedin Luković, Barbara Imperiali

Cliff Stains Publications

Recent efforts have identified the p38α Ser/Thr kinase as a potential target for the treatment of inflammatory diseases as well as non-small cell lung carcinoma. Despite the significance of p38α, no direct activity probe compatible with cell lysate analysis exists. Instead, proxies for kinase activation, such as phosphospecific antibodies, which do not distinguish between p38 isoforms, are often used. Our laboratory has recently developed a sulfonamido-oxine (Sox) fluorophore that undergoes a significant increase in fluorescence in response to phosphorylation at a proximal residue, allowing for real-time activity measurements. Herein we report the rational design of a p38α-selective chemosensor using this …

Synthesis Of Knowledge Of Extreme Fire Behavior: Volume I For Fire Managers, Paul Werth, Brian Potter, Craig Clements, Mark Finney, Scott Goodrick, Martin Alexander, Miguel Cruz, Jason Forthofer, Sara Mcallister

Faculty Publications, Meteorology and Climate Science

The National Wildfire Coordinating Group definition of extreme fire behavior (EFB) indicates a level of fire behavior characteristics that ordinarily precludes methods of direct control action. One or more of the following is usually involved: high rate of spread, prolific crowning/ spotting, presence of fire whirls, and strong convection column. Predictability is difficult because such fires often exercise some degree of influence on their environment and behave erratically, sometimes dangerously. Alternate terms include “blow up” and “fire storm.” Fire managers examining fires over the last 100 years have come to understand many of the factors necessary for EFB development. This …

Spin Transition In Gd3n@C-80, Detected By Low-Temperature On-Chip Squid Technique, L. Chen, E. E. Carpenter, C. S. Hellberg, H. C. Dorn, M. Schultz, W/ Wernsdorfer, I. Chiorescu

Chemistry Publications

We present a magnetic study of the Gd₃N@C₈₀ molecule, consisting of a Gd-trimer via a nitrogen atom, encapsulated in a C₈₀ cage. This molecular system can be an efficient contrast agent for magnetic resonance imaging (MRI) applications. We used a low-temperature technique able to detect small magnetic signals by placing the sample in the vicinity of an on-chip SQUID. The technique implemented at the National High Magnetic Field Laboratory has the particularity of being able to operate in high magnetic fields of up to 7 T. The Gd₃N@C₈₀shows a paramagnetic behavior and …

Town Report Milford, New Hampshire 2010., Milford Town Representatives

Milford, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

Central Washington University 2011-2012 Undergraduate/Graduate Catalog, Central Washington University

Course Catalogs

No abstract provided.

Direction Of The Play: The Outsiders, Janice Carroll

Graduate Student Projects

This project entailed the selection, background research and documentation, musical analysis, casting, vocal coaching, direction, and post-production analysis of Howe Sound Secondary School's production of Janice Carroll's The Outsiders. Documentation included research and analysis of the play and an evaluation of the play as a production vehicle for Theatre Arts at Central Washington Unviersity