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Epigenetic Effects Of Benzo(A)Pyrene In Fundulus Heteroclitus And Danio Rerio, Xiefan Fang

Electronic Theses and Dissertations

DNA methylation is one of the epigenetic mechanisms that controls gene expression and is vulnerable to early life environmental toxicant exposures. Our goal was to use two fish models, Fundulus heteroclitus and Danio rerio (zebrafish), to study the benzo(a)pyrene (BaP) effects on DNA methylation status and whether the alterations could contribute to BaP-mediated reproductive and developmental toxicities. Initially, we used Fundulus to study BaP effects on glycine N-methyltransferase (GNMT) expression throughout development. Fundulus embryos were exposed to waterborne BaP at nominally 10 or 100 μg/L and both GNMT mRNA expression and enzyme activity were measured. Quantitative PCR and whole mount …

Swinburne's "The Tale Of Balen": An Edition With Critical Commentary, Warren Hill Kelly

Electronic Theses and Dissertations

Warren Hill Kelly's Swinburne's The Tale of Balen: an Edition with Critical Commentary comprises a variorum-like edition of the poem that records all variances of the poem evident in its manuscript through its first and second editions, Chatto & Windus 1896 and 1904, or those editions produced during the poet's lifetime and therefore potentially bearing evidence of his editorial input. The edition forms as its basis the poet's final intention expressed in the manuscript, and notes all alterations within the manuscript and the first two published editions, and by coupling the edition's text with the notations pertaining to the manuscript, …

The Moral Responsibility Of The Corporate Lawyer, Judith A. Mcmorrow, Luke M. Scheuer

Catholic University Law Review

No abstract provided.

Monitoring Soil Quality To Assess The Sustainability Of Harvesting Corn Stover, Douglas L. Karlen, Gary Varvel, Jane M.F. Johnson, John M. Baker, S. L. Osborne, Jeff M. Novak, Paul R. Adler, Gregg Rothermel, Stuart Birrell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Harvesting feedstock for biofuel production must not degrade soil, water, or air resources. Our objective is to provide an overview of field research being conducted to quantify effects of harvesting corn (Zea mays L.) stover as a bioenergy feedstock. Coordi- nated field studies are being conducted near Ames, IA; St. Paul and Morris, MN; Mead, NE; University Park, PA; Florence, SC; and Brookings, SD., as part of the USDA-ARS Renewable Energy Assessment Project (REAP). A baseline soil quality assessment was made using the Soil Management Assessment Framework (SMAF). Corn grain and residue yield for two different stover harvest rates (~50% …

Mediation Of Host Immune Responses After Immunization Of Neonatal Calves With A Heat-Killed Mycobacterium Avium Subsp. Paratuberculosis Vaccine, Judith R. Stabel, W.R. Waters, John Bannantine, Konstantin P. Lyashchenko

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A major drawback of current whole-cell vaccines for Mycobacterium avium subsp. paratuberculosis is the interference with diagnostic tests for bovine tuberculosis (TB) and paratuberculosis. The current study was designed to explore the effects of immunization with a heat-killed whole-cell vaccine (Mycopar) on diagnostic test performance and to characterize host immune responses to vaccination over a 12-month period. Neonatal dairy calves were assigned to treatment groups consisting of (i) controls, not vaccinated (n 􏰊 5), and (ii) vaccinates, vaccinated with Mycopar vaccine (n 􏰊 5). The results from this study demonstrated a rapid initiation of M. avium subsp. paratuberculosis-specific gamma interferon …

Induction Of B Cell Responses Upon Experimental Infection Of Neonatal Calves With Mycobacterium Avium Subsp. Paratuberculosis, Judith R. Stabel, John Bannantine, Shigetoshi Eda, Suelee Robbe-Austerman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The objective of this study was to determine if experimental infection of neonatal calves with Mycobacterium avium subsp. paratuberculosis would invoke changes in the percentages of total B cells in the peripheral blood mononuclear cell population and of subpopulations of B cells as determined by CD5, CD25, and CD45RO markers during a 12-month period. Experimental infection groups included control (noninfected), oral (infected with M. avium subsp. paratuberculosis strain K-10), oral/DXM (pretreatment with dexamethasone before oral inoculation), i.p. (intraperitoneal inoculation), and oral/M (oral inoculation with mucosal scrapings from a cow with clinical disease) groups. Over the course of the study, the …

Relationship Of Cooked-Rice Nutritionally Important Starch Fractions With Other Physicochemical Properties, James Patindol, Harmeet Guraya, Elaine Champagne, Ming-Hsuan Chen, Anna Mcclung

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Sixteen rice cultivars representing five cytosine-thymine repeat (CT_n) microsatellite genetic marker groups were analyzed for their cooked rice nutritionally important starch fractions (NISFs, which include rapidly digestible (RDS), slowly digestible (SDS), and resistant starch (RS)), basic grain quality indices (apparent amylose (AM), crude protein (CP), alkali spreading value (AS), and gel consistency (GC)), pasting characteristics, and thermal properties. Chemometric tools (bivariate correlation, principal component analysis, multiple linear regression, and partial least squares regression) were used to establish the association of NISF with other milled rice physicochemical properties. CT₁₁ was generally associated with high percentages of RS and …

Regulatory Control Of High Levels Of Carotenoid Accumulation In Potato Tubers, Xiangjun Zhou, Ryan Mcquinn, Zhangjun Fei, Anne-Marie A. Wolters, Joyce Van Eck, Charles Brown, James J. Giovannoni, Li Li

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Potato (Solanum tuberosum L.) tubers contain a wide range of carotenoid contents. To decipher the key factors controlling carotenoid levels in tubers, four potato lines (Atlantic, Désirée, 91E22 and POR03) were examined by a combination of biochemical, molecular and genomics approaches. These lines contained incremental levels of carotenoids, which were found to be associated with enhanced capacity of carotenoid biosynthesis as evident from norflurazon treatment. Microarray analysis of high and low carotenoid lines (POR03 versus Atlantic) revealed 381 genes that showed significantly differential expression. The carotenoid metabolic pathway genes b-carotene hydroxylase 2 (BCH2) and b-carotene hydroxylase 1 (BCH1), along …

Invited Commentary: Dietary Pattern Analysis, Fumiaki Imamura, Paul F. Jaques

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The analytic approaches used in nutritional epidemiology for dietary pattern analyses share common characteristics with those of genetic epidemiology. In this issue of the Journal, Gorst-Rasmussen et al. (Am J Epidemiol. 2011;173(10):1097–1104) discuss one such approach. Application of methods used in genetic pattern analyses to nutritional epidemiology could prove valuable but raises important issues that need to be considered because dietary and genetic studies often address different types of questions in analyzing interrelated variables. These different aims require statistical methods that assume different characteristics of the underlying patterns. The authors briefly describe such differences to facilitate interpretation and …

The Rural Growth Trifecta: Outdoor Amenities, Creative Class And Entrepreneurial Context, David A. Mcgranahan, Timothy R. Wojan, Dayton M. Lambert

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Recent work challenges the notion that attracting creative workers to a place is sufficient for generating local economic growth. In this article, we examine the problem of sustaining robust growth in the periphery of the USA, demonstrating the contingent nature of talent as an engine for economic growth. We test the hypothesis that rural growth in the knowledge economy is dependent on the ability to utilize new knowledge, perhaps generated elsewhere, in addressing local economic challenges. Tests confirm that the interaction of entrepreneurial context with the share of the workforce employed in the creative class is strongly associated with growth …

26th Hohenheim Consensus Conference, September 11, 2010 Scientific Substantiation Of Health Claims: Evidence-Based Nutrition, Hans Konrad Biesalski, Peter J. Aggett, Robert Anton, Paul S. Bernstein, Jeffrey Blumberg, Robert P. Heaney, Jeya Henry, John M. Nolan, David P. Richardson, Ben Van Ommen, Renger F. Witkamp, Ger T. Rijkers, Iris Zöllner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Objective: The objective was to define the term evidence based nutrition on the basis of expert discussions and scientific evidence.

Methods and procedures: The method used is the established Hohenheim Consensus Conference. The term “Hohenheim Consensus Conference” defines conferences dealing with nutrition-related topics. The major aim of the conference is to review the state of the art of a given topic with experts from different areas (basic science, clinicians, epidemiologists, etc.). Based on eight to 12 questions, the experts discuss short answers and try to come to a consensus. A scientifically based text is formulated that justifies the …

Response Of Tribolium Castaneum And Tribolium Confusum Adults To Vertical Black Shapes And Its Potential To Improve Trap Capture, Altair A. Semeao, James F. Campbell, R. Jeff Whitworth, Phillip E. Sloderbeck

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Tribolium castaneum and Tribolium confusum can be monitored in food processing facilities using traps baited with pheromones and kairomones, but beetle response to traps might be enhanced by adding visual cues. Against a white background, T. castaneum adults were more likely to visit black pillars than white pillars when presented with a choice (e.g., 73% of beetles visited black and 17% visiting white pillar), and visits to black pillars increased with pillar height. When tested against a black background, beetles did not show a significant preference for either color pillar regardless of height. When comparing beetle’s captures in pheromone/kairomone baited …

Effect Of Maternal Restraint Stress During Gestation On Temporal Lipopolysaccharide-Induced Neuroendocrine And Immune Responses Of Progeny, C.T. Collier, P.N. Williams, J.A. Carroll, T.H. Welsh Jr, J.C. Laurenz

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The impact of gestational dam restraint stress on progeny immune and neuroendocrine temporal hormone responses to lipopolysaccharide (LPS) challenge was assessed. Maternal stress (5-min snout snare restraint stress during days 84 to 112 of gestation) increased (P < 0.05) the magnitude of tumor necrosis factor (TNF)-α, interleukin-6, epinephrine (E), norepinephrine, and serum amyloid A (SAA) production following LPS infusion in the offspring. Moreover, these effects appear to be dependent on gender for TNF-α, E, and cortisol production. However, maternal stress did not affect (P < 0.05) the normalization of proinflammatory cytokines or neuroendocrine hormones produced following LPS. Collectively, these results indicate that maternal stress impacts aspects of the proinflammatory cytokine and stress hormone response in their progeny following LPS dosing of the offspring. This response is potentially responsible in part for the resultant changes to SAA production. Because several of the changes observed here are dependent on pig gender, these results are also the first evidence that inherent epigenetic factors coupled with maternal stress impact the cumulative response to stress and LPS in young pigs.

Evaluation Of Two Mutants Of Mycobacterium Avium Subsp. Paratuberculosis As Candidates For A Live Attenuated Vaccine For Johne’S Disease, Kun Taek Park, Andrew J. Allen, John P. Bannantine, Keun Seok Seo, Mary J. Hamilton, Gaber S. Abdellrazeq, Heba M. Rihan, Amanda Grimm, William C. Davis

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Control of Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis, has been difficult because of a lack of an effective vaccine. To address this problem we used targeted gene disruption to develop candidate mutants with impaired capacity to survive ex vivo and in vivo to test as a vaccine. We selected relA and pknG, genes known to be important virulence factors in Mycobacterium tuberculosis and Mycobacterium bovis, for initial studies. Deletion mutants were made in a wild type Map (K10) and its recombinant strain expressing the green fluorescent protein (K10-GFP). Comparison of survival in an ex vivo …

Improving In-Season Nitrogen Recommendations For Maize Using An Active Sensor, J. Schmidt, D. Beegle, Q. Q. Zhu, R. Sripada

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An active crop canopy reflectance sensor could be used to increase N-use efficiency in maize (Zea mays L.), if temporal and spatial variability in soil N availability and plant demand are adequately accounted for with an in-season N application. Our objective was to evaluate the success of using an active canopy sensor for developing maize N recommendations. This study was conducted in 21 farmers’ fields from 2007 to 2009, representing the maize production regions of east central and southeastern Pennsylvania, USA. Four blocks at each site included seven sidedress N rates (0–280 kgNha⁻¹) and one at-planting N …

Oil Productivity And Composition Of Sunflower As A Function Of Hybrid And Planting Date, Valtcho D. Zheljazkov, Brady A. Vick, Brian S. Baldwin, Normie Buehring, Christine Coker, Tess Astatkie, Billy Johnson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Sunflower (Helianthus annuus L.) is a potential cash crop for the southeastern United States for production of cooking oil or biodiesel. Two years (2006 and 2007) of experiments were conducted at each of five locations in Mississippi to evaluate the effect of planting date (April 20, May 20, and June 20), and hybrid (DKF3875, DKF2990, DKF3510, DKF3901, PR63M80, PR62A91, PR63A21, PR63M91, and PR64H41) on seed yield, oil content, and oil composition of sunflower. Seed oil concentration varied from 25 to 47%. The oleic acid concentration in the oil was greater than 85% for DKF3510 and PR64H41, above 65% for …

Improving Animal Welfare And Reducing Animal Use For Veterinary Vaccine Potency Testing: State Of The Science And Future Directions, William S. Stokes, Karen Brown, Jodie Kulpa-Eddy, Geetha Srinivas, Marlies Halder, Hans Draayer, Jeffrey Galvin, Ivo Claassen, Glen Gifford, Ralph Woodland, Vivian Doelling, Brett Jones

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Veterinary vaccines contribute to improved human and animal health and welfare by preventing diseases and deaths caused by a wide range of infectious agents. However, testing necessary to ensure vaccine effectiveness and safety can involve large numbers of animals and significant pain and distress. NICEATM and ICCVAM convened an international workshop to review the state of the science of human and veterinary vaccine potency and safety testing methods and to identify opportunities to advance new and improved methods that can further reduce, refine, and replace animal use. This workshop report is the fourth in a series of six, and addresses …

A Spatially Explicit Degree-Day Model Of Rift Valley Fever Transmission Risk In The Continental United States, Sarah K. Konrad, Scott N. Miller, Will K. Reeves

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A spatially explicit degree-day model was used to evaluate the risk of Rift Valley fever virus (RVFV) transmission by mosquitoes to humans and livestock within five target states in the continental United States: California, Minnesota, Nebraska, New York, and Texas. A geographic information system was used to model potential virus transmission based on a 12-day moving window assessment of the extrinsic incubation period theorized for RVFV in the United States. Risk of potential virus transmission in each state was spatially evaluated on a 10-km grid using average historical daily temperature data from 1994 to 2003. The highest levels of transmission …

How Might Stress Contribute To Increased Risk For Schizophrenia In Children With Chromosome 22q11.2 Deletion Syndrome?, Elliott A. Beaton, Tony J. Simon

Psychology Faculty Publications

The most common human microdeletion occurs at chromosome 22q11.2. The associated syndrome (22q11.2DS) has a complex and variable phenotype with
a high risk of schizophrenia. While the role of stress in the etiopathology of schizophrenia has been under investigation for over 30 years (Walker et al. 2008), the stress–diathesis model has yet to be investigated in children with
22q11.2DS. Children with 22q11.2DS face serious medical, behavioral, and socioemotional challenges from infancy into adulthood. Chronic stress elevates glucocorticoids, decreases immunocompetence, negatively impacts brain
development and function, and is associated with psychiatric illness in adulthood. Drawing knowledge from the extant and well-developed …

The Seaside Resort Towns Of Cape May And Atlantic City, New Jersey Development, Class Consciousness, And The Culture Of Leisure In The Mid To Late Victorian Era, Tatyana Ressetar

Electronic Theses and Dissertations

"Victorianism" is a highly controversial, sometimes ironic, term penned by historians throughout various works that has come to hold dramatic weight in both its meaning and its influence. Though the term is usually most closely associated with nineteenth century England, Victorianism was a highly influential movement in American culture simultaneously as well, specifically in the spheres of home, work, and play. Of those, "play," or leisure, is undoubtedly the least explored, especially before the latter decades of the twentieth century. Prior to this period, most literature about the Victorians, with the exception of a few works, only dealt with masculinity, …

The Synergies Between Tourism Expansion And Development An Analysis Of Economic Growth, Poverty And Human Development In Ecuador, Manuel Antonio Rivera

Electronic Theses and Dissertations

This dissertation attempts to answer the question of what has been the effect of tourism expansion and development in Ecuador. We attempt to analyze, both theoretically and empirically, the relationship between tourism and development. There have been an enormous number of studies explaining how tourism can affect the various dimensions of development. However, no research has been undertaken to simultaneously empirically examine the impact that changes in tourism expansion has on poverty, human development, and economic growth. To this end, we propose a cointergation methodology with an error correction model to estimate the impact of tourism expansion on poverty, human …

Democracy And Education Equity In Latin America, Olen Dean Stonerook

Electronic Theses and Dissertations

In the literature democratic longevity in countries transitioning from authoritarian regimes to democracy is linked to economic development; four factors of economic development are identified: industrialization, education, urbanization, and growing wealth. Education is viewed as a primary factor for effective democratic participation and economic development. This thesis examines the relationship between level of democracy and educational outputs and outcomes. Does the level of democracy (political rights and civil liberties) have an effect on the levels of investment in education and measurable outcomes in education equity toward meeting the educational needs of the newly represented public? The expectation is that the …

Adherence Practices Of Caucasian Women With Hypertension Residing In Rural Florida An Exploratory Study, Jeanne M. Hopple

Electronic Theses and Dissertations

Hypertension, or high blood pressure, is a major risk factor for heart disease and stroke. Elevated blood pressure is often a silent process affecting multiple organ systems. Risk for heart disease is associated with poorly treated or unrecognized hypertension that is more common among women than men. Non-adherence to prescribed treatment regimens has been identified as a major reason for inadequate hypertension management. This exploratory descriptive qualitative study using narrative inquiry investigated adherence practices among Caucasian women with diagnoses of hypertension from a rural area of Florida. The purpose of this study was to gain an understanding from women who …

Differential Diagnoses Of Temporal Bone Defects And Zygomatic Bone Lesions Found In Fetal And Infant Individuals From The Kellis 2 Cemetery, Dakhleh Oasis, Egypt, Brittany A. Jardine

Electronic Theses and Dissertations

The Kellis 2 cemetery site within the Dakhleh Oasis, Egypt provides a unique study opportunity due to the large number of infant, perinatal, and fetal individuals that have been recovered. Several of the infant and fetal remains have undiagnosed circular defects on the temporal bone, and others have undiagnosed lesions on the zygomatic bone. Of the 268 individuals under one year of age that have been analyzed from the Kellis 2 cemetery, twentysix individuals have the temporal bone defect and six have the zygomatic bone lesions. A survey of clinical and paleopathological research provided possible pathological conditions that could cause …

Stable Expression Of Tuberculosis Vaccine Antigen In Lettuce Chloroplasts, Priya Saikumar Lakshmi

Electronic Theses and Dissertations

Tuberculosis (TB) is caused by Mycobacterium tuberculosis and is one of the leading reasons of death by an infectious bacterial pathogen. The development of TB vaccines has been recognized as a major public health priority by the World Health Organization. In this study, a potential candidate antigen, ESAT-6 (6 kDa early secretory antigenic target) was fused with cholera toxin B subunit (CTB). Transplastomic lettuce plants were generated expressing these fusion proteins. Site-specific transgene integration into the chloroplast genome was confirmed by polymerase chain reaction and Southern blot analysis. In transplastomic leaves, expression levels of fusion protein (CTB-ESAT6) varied depending upon …

Evolutionary Relationships Among Staphylococci And The Prevention Of Staphylococcus Aureus Nasal Colonization, Ryan Paul Lamers

Electronic Theses and Dissertations

Staphylococcus is a significant cause of human infection and mortality, worldwide. Currently, there are greater than 60 taxa within Staphylococcus, and nearly all are pathogenic. The collective potential for virulence among species of Staphylococcus heightens the overall clinical significance of this genus and argues for a thorough understanding of the evolutionary relationships among species. Within Staphylococcus, aureus is the most common cause of human infection, where nasal carriage of this bacterium is a known risk factor for autoinfection. The predisposition to infection by nasal carriers of S. aureus, and the ease with which strains are transferred between individuals, suggests that …

An Investigation Of The Representation Of Middle School English Language Learners (Ells) In Special Education Programs In A Large Urban School District, Leah J. Lerma

Electronic Theses and Dissertations

The disproportionate representation of ethnically and racially diverse students in special education has been an enduring problem in education for the past four decades. However, most of the research on disproportionality has focused on the ethnic/racial variable; the body of research focusing on the linguistic variable is still slim in comparison. As linguistic diversity in the United States continues to rise, teachers will continue to be challenged to meet the needs of the English Language Learners in their classrooms. However, many teachers feel unprepared to deal with this diversity and have difficulty discriminating whether a student’s poor performance is due …

Management Support And Faculty's Adoption Of Learning Management System Applying Technology Acceptance Model 3, Zhigang Li

Electronic Theses and Dissertations

The purpose of this research study was to understand and identify the key factors that affect faculty’s behavioral intention of using a learning management system. This research study adopted the Technology Acceptance Model 3 (TAM3) as the theoretical foundation and extended it by adding management support as an exogenous variable based on the recommendations from previous research studies. Technology Acceptance Model 3 is the latest iteration of Technology Acceptance Model – a widely adopted research framework for studying users’ acceptance of technology. It provides a comprehensive network of determinants of technology adoption and use. A survey questionnaire with 54 measurement …

The Effects Of Urbanization On Cypress (Taxodium Distichum) In Central Florida, Lisa A. Mccauley

Electronic Theses and Dissertations

Urbanization is accelerating in the United States and is contributing to fragmentation of natural habitats, causing changes in species composition and declines in native species. Human population growth in Orlando is typical of growth in the southeastern United States and throughout the range of cypress (Taxodium distichum). Orlando has numerous isolated cypress wetlands, called cypress domes, and many remain among the current urbanized area. This makes Orlando ideal to study the effects of urbanization on cypress domes. Specifically, I tested how urbanization and its effects on fragmentation, hydrology, and fire regime) affected (a) the numbers and spatial pattern of cypress …

Effects Of A Reading Inference Strategy Intervention On The Reading And Social Inference Abilities Of Adults With Asperger Syndrome, Kimberly A. Murza

Electronic Theses and Dissertations

The ability to generate inferences is a skill that is necessary to fully comprehend a text and understand the intentions, behaviors, and emotions of a conversational partner. Individuals with Asperger syndrome (AS) have been shown to demonstrate significant difficulty in inference generation in both social contexts and in reading comprehension. Although, the reciprocity of the four components of literacy (reading, writing, listening, and speaking) has been established in the literature (Bradley & Bryant, 1983; Catts & Kamhi, 2005; Englert & Thomas, 1987; Gillon & Dodd, 1995; Hiebert, 1980; Kroll, 1981; Ruddell & Ruddell, 1994); the relationship between inference generation in …