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2011

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Assessment Of Bacterial Community Patterns From Ecologically Distinct Antarctic Soil Environments / Chun Wie Chong, Chun Wie Chong Jan 2011

Assessment Of Bacterial Community Patterns From Ecologically Distinct Antarctic Soil Environments / Chun Wie Chong, Chun Wie Chong

Student Works (2010-2019)

It is commonly known that bacteria are ubiquitously distributed in soil environments. They are heavily involved in the biogeochemical cycles and are vital in maintaining soil ecological functions. These functional roles of bacteria are especially important in the extreme and harsh environments of Antarctica where the terrestrial trophic interactions are generally limited to microorganisms due to restricted development of terrestrial plant and animal communities. In order to assess the spatial distribution and taxonomic composition of soil bacterial community in Antarctica, soils were collected from a range of geographically and environmentally distinct locations. These included relatively milder but variable environments on …

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Mycotoxigenic Fungi And Mycotoxins Contaminating Red Rice At Consumer Level In Selangor, Malaysia / Nik Iskandar Putra Samsudin, Samsudin Nik Iskandar Putra Jan 2011

Mycotoxigenic Fungi And Mycotoxins Contaminating Red Rice At Consumer Level In Selangor, Malaysia / Nik Iskandar Putra Samsudin, Samsudin Nik Iskandar Putra

Student Works (2010-2019)

Red rice is a product of rice fermentation by fungi from the genus Monascus notably the M. purpureus, M. ruber, and M. pilosus. Although the traditional method of its preparation does not necessarily adhere to safety and health regulation, it is still widely consumed by Chinese as traditional herbal remedy believed to possess outstanding and diverse industrial, clinical, and food applications. The present study was undertaken to determine the occurrence of mycotoxigenic fungi and mycotoxins present on red rice at consumer level in Selangor, Malaysia. In this study, 50 samples of red rice were obtained from nine administrative districts of …

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Determination And Assessment Of Potential Mobility Of Heavy Metals In Solid Samples By Sequential Extraction Procedures (Sep) / Keivan Nemati, Keivan Nemati Jan 2011

Determination And Assessment Of Potential Mobility Of Heavy Metals In Solid Samples By Sequential Extraction Procedures (Sep) / Keivan Nemati, Keivan Nemati

Student Works (2010-2019)

The use of sequential extraction schemes for fractionation of metals in different substrates has grown exponentially over the past two decades. Despite the popularity of these schemes, they have been criticized for metal redistribution and the reagents used. The work presented in this dissertation is aimed at addressing these problems by, (i) a Tessier sequential extraction (ii) a BCR sequential extraction schemes and modified BCR method, (iii) a comparison between Tessier and BCR method for shrimp aquaculture sludge. From the sequential extraction, Cd, Mn and Pb were mostly found in exchangeable/carbonate form, showed its susceptibility to be leached easily. Also …

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Chromosomal Alterations In Oral Cancer For Selected Indian And Indigenous Population / Noor Julieana Mat Amin, Mat Amin Noor Julieana Jan 2011

Chromosomal Alterations In Oral Cancer For Selected Indian And Indigenous Population / Noor Julieana Mat Amin, Mat Amin Noor Julieana

Student Works (2010-2019)

Introduction: In the Malaysian population, the Indians were identified as having a higher oral cancer (OC) risk compared to Malays, Chinese and other ethnicities with betel quid chewing contributing the major factor among Indian female. Cancer progresses through a series of histopathological stages where the progression is thought to be driven by the accumulation of genetic alterations (Garnis et. al., 2009). Therefore, the identification of chromosomal alterations will not only enhance the understanding of the biology in this process, but will also identify important genes that might involve in oral carcinogenesis. Thus, this study utilised array Comparative Genomic Hybridization (arrayCGH) …

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Mining And Validation Of Est-Microsatellites In Freshwater Prawns, Macrobrachium Rosenbergii / Shairah Abdul Razak, Abdul Razak Shairah Jan 2011

Mining And Validation Of Est-Microsatellites In Freshwater Prawns, Macrobrachium Rosenbergii / Shairah Abdul Razak, Abdul Razak Shairah

Student Works (2010-2019)

The current study conducted in purpose to illustrate the utility of EST-derived SSR in characterizing wild populations of M. rosenbergii in Malaysia‟s rivers. A novel set of EST-SSR generated from RNA of M. rosenbergii were validated in a full panel of 120 samples from four wild populations through Polymerase Chain Reaction (PCR). Seven EST-SSR loci were identified, characterized, and evaluated on 30 individuals each from the populations namely Negeri Sembilan, Kedah, Sarawak, and Terengganu. The average polymorphic informative content value (PIC) for these seven primers was found to be 0.6208 indicating considerable degree of polymorphism with number of alleles detected …

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Evaluation Of Obturation Quality Using Gutta-Percha And A Resin-Based Material With Different Techniques / Nashwan Abdullah Abdo Al-Afifi., Nashwan Abdullah Abdo Al-Afifi Jan 2011

Evaluation Of Obturation Quality Using Gutta-Percha And A Resin-Based Material With Different Techniques / Nashwan Abdullah Abdo Al-Afifi., Nashwan Abdullah Abdo Al-Afifi

Student Works (2010-2019)

Introduction: Gutta-percha (GP) has been accepted as the “gold standard” root filling material. It is the material against which most others are compared. The adhesive potential of GP to radicular dentine has been shown to be far from satisfactory. Therefore, resin-based materials that address many of the limitations of the GP/sealer combinations have been introduced. They are claimed to produce the so-called “monoblock” that is a gap-free union between core material and sealer, which adheres and penetrates into dentinal tubules. Examples of these resin-based filling materials include Resilon, EndoREZ and Guttaflow. Objectives: The objectives of this study were to evaluate …

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Distributions Of Extractable Organic Compounds In Airborne Paraticulate Matter Over The Straits Of Malacca, South China Sea And Sulu-Sulawesi Seas / Aliaa Diyana Azizuddin, Azizuddin Aliaa Diyana Jan 2011

Distributions Of Extractable Organic Compounds In Airborne Paraticulate Matter Over The Straits Of Malacca, South China Sea And Sulu-Sulawesi Seas / Aliaa Diyana Azizuddin, Azizuddin Aliaa Diyana

Student Works (2010-2019)

The solvent-extractable hydrocarbons of 26 atmospheric samples, collected during a 2 months cruise starting from the Straits of Malacca, South China Sea and ends at Sulu- Sulawesi Seas were analyzed to determine the sources and distributions of homologous compounds series. The atmospheric aerosols were collected using a standard high volume air sampler at the rate of 1.13 m3/min for 24 hours. The filters with suspended particles were then extracted by ultrasonic agitation with dichloromethane and analyzed using gas chromatography-mass spectrometry. The particulate matter contains mainly fatty acids, n-alkanes and low levels of n-alcohols, n-aldehydes and aliphatic ketones derived from both …

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Association Of Dna Repair Gene Polymorphism (Xrcc1) And Oral Cancer Risk / Mohadeseh Hasanpourghadi, Mohadeseh Hasanpourghadi Jan 2011

Association Of Dna Repair Gene Polymorphism (Xrcc1) And Oral Cancer Risk / Mohadeseh Hasanpourghadi, Mohadeseh Hasanpourghadi

Student Works (2010-2019)

The main aim of the present study was to determine the distribution of XRCC1 Arg399Gln genotypes in oral cancer and non-oral cancer patients. This study was also to investigate the association between XRCC1 Arg399Gln genotypes and oral cancer risk and to compare the distribution of XRCC1 Arg399Gln genotypes among different ethnic groups in Malaysia. This case-control study involved a total of 209 cases of oral squamous cell carcinoma (OSCC) patients and 212 controls with neither any trace of cancers nor any family history of cancer. Mean age of the OSCC patients for cases was 61.34 ± 14.01 years, while for …

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Coral Ecology Of A Unique Continental Island Of Pulau Perak, Kedah, Malaysia / Affendi Yang Amri, Yang Amri Affendi Jan 2011

Coral Ecology Of A Unique Continental Island Of Pulau Perak, Kedah, Malaysia / Affendi Yang Amri, Yang Amri Affendi

Student Works (2010-2019)

Coral reef research in Peninsular Malaysia has mainly been concentrated on the east coast with little emphasis on the Straits of Malacca (SOM) in the west coast. The coral reefs in SOM are extremely important, more so than their east coast counterparts due to the fact that they are only a few viable coral reef ecosystems in SOM. In the northern part of SOM stands Pulau Perak, an island which has underwater vertical reef walls. Pulau Perak reef walls are unique compared to other continental islands in Peninsular Malaysia which have typical fringing reefs. In addition the scientific knowledge of …

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Screening And Partial Purification Of Protease Inhibitors From Senna Surattensis Leaves / Ahmad Firdhaus Arham., Arham Ahmad Firdhaus Jan 2011

Screening And Partial Purification Of Protease Inhibitors From Senna Surattensis Leaves / Ahmad Firdhaus Arham., Arham Ahmad Firdhaus

Student Works (2010-2019)

This study was done to determine the inhibitory activity that were exhibited by different parts of the local plants in Malaysia selected from the 4 families which are Leguminosae, Rubiaceae, Apocynaceae and Euphorbiaceae. The screening method used in this study are Bradford Assay and Trypsin Inhibitory Assay. Both these assays revealed that Senna surattensis leaves showed the highest inhibitory activity of 83 % compared to other 41 plant samples studied in this study. SDS-PAGE and Tricine SDS on this sample extract showed the presence of this protease inhibitor through the formation of band. From these band also, the molecular weight …

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Analysis Of Transgenic Wild Rice : Molecular Confirmation Of Transgene Presence And Risk Assessment For Pollen Viability / Alireza Saeidi., Alireza Saeidi Jan 2011

Analysis Of Transgenic Wild Rice : Molecular Confirmation Of Transgene Presence And Risk Assessment For Pollen Viability / Alireza Saeidi., Alireza Saeidi

Student Works (2010-2019)

Rice is one of the major sources of food for the population of the world, and almost 50% of the world population depends on it. Malaysian national rice production was estimated to provide just 60-65% of the domestic requirement, so the strategies that can help to increase rice yield are necessary for the agricultural sector of Malaysia. Towards this, one useful research approach is to reduce or knock down expression of a rice gene using RNA interference (RNAi) with the goal of obtaining a phenotype that is suggestive of its function. This project is a based on the analysis of …

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Biological Activities Of Alpinia Pahangensis And Alpinia Mutica Extracts / Fadhlina Binti Mahfot, Mahfot Fadhlina Jan 2011

Biological Activities Of Alpinia Pahangensis And Alpinia Mutica Extracts / Fadhlina Binti Mahfot, Mahfot Fadhlina

Student Works (2010-2019)

The aim of the present study is to investigate the biological activities of two selected wild Alpinia species namely, Alpinia pahangensis Ridl. and Alpinia mutica Roxb. Extraction of the rhizomes and leaves was done successively using three solvents with different polarity: hexane, dichloromethane and methanol. The crude extracts were tested for their antimicrobial and antioxidant activities. The antioxidant activity was determined using the DPPH free radical scavenging method. The antimicrobial activity was investigated based on disc-diffusion assay and minimum inhibition concentration (MIC) against 12 microorganisms consisting of six gram positive bacteria: Bacillus cerues, Bacillus subtilis, Staphylococcus aureus, Streptococus mutans, Streptococcus …

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Comparative Studies Of Bioethanol Fuel Production And Engine Emission From Different Rotten Fruits Biomasses / Hadeel Mustafa Al-Yamani, Hadeel Mustafa Al-Yamani Jan 2011

Comparative Studies Of Bioethanol Fuel Production And Engine Emission From Different Rotten Fruits Biomasses / Hadeel Mustafa Al-Yamani, Hadeel Mustafa Al-Yamani

Student Works (2010-2019)

The unsustainable energy resources like fossil fuel are going to be depleted in the next few decades and the trends in biofuel production from biomass are gaining popularity to encounter the expected energy crisis in the world. Ethanol is the most widely used liquid biofuel and is produced as a result of fermentation process from sugars, starches or cellulosie biomass including fruit wastes. In this study rotten rambutan, mango, banana and pineapple fruits were utilized for bioethanol production. Rotten fruits were used to produce bioethanol by fermentation using yeast (Saccromyces cerevisiae ). Different parameters including pH, TSS, glucose concentration, retention …

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Genetic Variation And Differentiation Between Cultured And Wild Populations Of Channa Striata (Haruan) From Microsatellite Markers / Jothi Annavadivoo A/P Balasingam., Jothi Annavadivoo Balasingam Jan 2011

Genetic Variation And Differentiation Between Cultured And Wild Populations Of Channa Striata (Haruan) From Microsatellite Markers / Jothi Annavadivoo A/P Balasingam., Jothi Annavadivoo Balasingam

Student Works (2010-2019)

The snakehead fish (Channa striata) is a freshwater fish species indigenous to Malaysia. Channa striata (C. striata), lo cally known as Haruan is valued as a natural remedy in traditional medicine as well as a reputable source of protein. The rapid progress of scientific validation research on the therapeutic properties of the species has spurred the equally rapid expansion of commercialised products that capitalises on these unique yet beneficial qualities of C. striata. Dependence on the wild in order to meet the increasing demand on the species is no longer a feasible option whereby, deteriorating natural abundance due to anthropogenic …

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Seagrass Mapping And Distribution In Relation To Water Quality Using Gis In Sungai Johor, Peninsular Malaysia / Noor Aini Ahmad, Noor Aini Ahmad Jan 2011

Seagrass Mapping And Distribution In Relation To Water Quality Using Gis In Sungai Johor, Peninsular Malaysia / Noor Aini Ahmad, Noor Aini Ahmad

Student Works (2010-2019)

Seagrass meadows are noted for their extremely high productivity and contribution of valuable ecosystem functions and services to coastal zone. Seagrasses which are vulnerable to the changes of water and environmental quality are increasingly threatened by human activities resulting in a deterioration of water quality. An environmental study on seagrass was conducted at Sungai Johor, Peninsular Malaysia. The purpose of this study was to examine the relationship between species diversity and distribution of seagrasses and various environmental (water quality) factors. Three (3) seagrass species were found along Sungai Johor which identified as Halophila ovalis, Halophila spinulosa and Enhalus acoroides. Halophila …

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Antioxidant And Cytotoxic Investigations Of Beta Vulgaris L / Jaime Stella A/P Moses Richardson, Jaime Stella Moses Richardson Jan 2011

Antioxidant And Cytotoxic Investigations Of Beta Vulgaris L / Jaime Stella A/P Moses Richardson, Jaime Stella Moses Richardson

Student Works (2010-2019)

Beta vulgaris has been commonly consumed and traditionally used for various medicinal purposes. The present study scientifically evaluates the antioxidant and cytotoxic potential of the plant using various bioassays. The root part of Beta vulgaris was collected and extracted using methanol and then fractionated with hexane, ethyl acetate and water. The fresh root of Beta vulgaris was juiced and then subjected to evaporation under reduced pressure to form an extract. Chemical investigations were then directed to the ethyl acetate fraction and juice extract; fraction ET1, ET2 and ET3 were obtained from ethyl acetate fraction by isolation using high performance liquid …

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Proteomic Characterization Of Eurycoma Longifolia And Its Application In Validating Tongkat Ali Herbal Products / Venoos Iman, Venoos Iman Jan 2011

Proteomic Characterization Of Eurycoma Longifolia And Its Application In Validating Tongkat Ali Herbal Products / Venoos Iman, Venoos Iman

Student Works (2010-2019)

Eurycoma longifolia commonly known as “Tongkat Ali” in Malaysia is claimed to have aphrodisiac properties and has been widely used in Malay traditional medicine. An aphrodisiac is defined as a substance that increases sexual activity and libido and/or improves sexual performance. These claims have led to a vast number of “Tongkat Ali” products being marketed without proper authentication. There was therefore a need to characterize the proteins in these commercial Tongkat Ali herbal products using biomarkers to establish the authenticity of the claims. The important chemical components in Eurycoma longifolia are eurycomanol, eurycomanone and eurycomalactone. However they are not linked …

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Discovery Of Immune Responsive Proteins In Ihhnv-Infected And Non-Infected Macrobrachium Rosenbergii / Tahereh Alinejad, Tahereh Alinejad Jan 2011

Discovery Of Immune Responsive Proteins In Ihhnv-Infected And Non-Infected Macrobrachium Rosenbergii / Tahereh Alinejad, Tahereh Alinejad

Student Works (2010-2019)

The major problem in the prawn industry worldwide is acute epizootic diseases that are closely associated with explosive death among prawns which leads to economic losses in commercial aquaculture and posing threats to the indigenous wild stocks. Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) was listed by OIE (Office of International Epizootics of World Organization for Animal Health) to be shown to have international significance in aquaculture. The study was conducted to understand the molecular responses of crustacean hemocytes to IHHNV infection. 2D-proteomic approach was used to investigate the differential expression proteins in hemocytes of infected and non-infected M.rosenbergi .Viral …

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Mathematical Model On The Cellulase Enzymes Production As A Function Of Viscosity / Fahimeh Abdeyazdan, Fahimeh Abdeyazdan Jan 2011

Mathematical Model On The Cellulase Enzymes Production As A Function Of Viscosity / Fahimeh Abdeyazdan, Fahimeh Abdeyazdan

Student Works (2010-2019)

As cellulosic material is the most abundant and renewable source of organic matter, ethanol can be produced cheaply from cellulosic waste. The cellulose must be hydrolyzed to glucose before it can be fermented to ethanol. The enzymatic hydrolysis of cellulose using cellulase enzymes is considered an environmental- friendly and less energy intensive alternative. White-rot fungi belong to the wood-destroying basidiomycetes and are well known as the only micro-organisms responsible for the enzyme hydrolysis of all major wood polymers, including lignin, cellulose and hemicelluloses. On the other hand, the production cost of cellulase enzymes is a limitation in ethanol fermentation. Therefore, …

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Production Of Caprine And Bovine In Vitro-Fertilised As Well As Parthenogenetic Embryos And An Attempt To Vitrify In Vivo- And In Vitro-Derived Embryos / Tan Wei Lun, Wei Lun Tan Jan 2011

Production Of Caprine And Bovine In Vitro-Fertilised As Well As Parthenogenetic Embryos And An Attempt To Vitrify In Vivo- And In Vitro-Derived Embryos / Tan Wei Lun, Wei Lun Tan

Student Works (2010-2019)

The main objective of this research was to determine the effects of oocyte grading and insemination duration on cleavage rate of embryos obtained from in vitro fertilisation (IVF) in bovine and caprine species. In addition, the effects of oocyte grading in bovine and caprine on production of parthenogenetic embryos as well as an attempt to cryopreserve embryos using vitrification technique were also evaluated. Bovine oocytes were obtained from abattoir while caprine oocytes were retrieved through laparoscopic oocyte pick-up (LOPU) or abattoir source. For laparoscopic oocyte pick-up goats, gonadotrophin injections were involved prior to surgery, which were Estrumate (125 μg), Pregnant …

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Preparation Of Β-Cyclodextrin Based Adsorbents And Their Behaviour Towards Chromium(Vi)-Diphenylcarbazide Complex Removal From Aqueous Solution / Nurul Huda Mohd Yusof, Nurul Huda Mohd Yusof Jan 2011

Preparation Of Β-Cyclodextrin Based Adsorbents And Their Behaviour Towards Chromium(Vi)-Diphenylcarbazide Complex Removal From Aqueous Solution / Nurul Huda Mohd Yusof, Nurul Huda Mohd Yusof

Student Works (2010-2019)

The adsorption of Cr(VI) ions from aqueous solution by β-cyclodextrin (β-CD) based polymer as an adsorbent was studied in a batch adsorptions experiments. Three types of adsorbent were synthesized, namely, β-CDCP-HMDI, β-CDCP-TDI and Si-TDI-βCD. The method was based on the complex reaction of Cr(VI) with 1,5-diphenylcarbazide (DPC) in acidic solution loaded on β-CDCP-HMDI and β-CDCP-TDI and Si-TDI-βCD. The adsorption isotherm and kinetics of the three adsorbent were studied. Equilibrium isotherm was analyzed according to classic Langmuir and Freundlich model. It shows that the Langmuir isotherm is better in describing the adsorption process for adsorbent β-CDCP-HMDI, while Freundlich isotherm is better …

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Biologically Active Alkaloids From Alstonia Angustiloba / Ku Wai Foong, Wai Foong Ku Jan 2011

Biologically Active Alkaloids From Alstonia Angustiloba / Ku Wai Foong, Wai Foong Ku

Student Works (2010-2019)

The alkaloidal content of Malaysian plant, Alstonia angustiloba Miq., was investigated and the results are summarized as below (Table). A total of 20 alkaloids were isolated and characterized, of which, three were new alkaloids, viz., angustilobine C (1), andransinine (2), and angustiphylline (3). Angustilobine C (1) and andransinine (2) are alkaloids of the angustilobine- and andranginine type, respectively, while angustiphylline (3) is a new bisindole alkaloid constituted from the union of uleine and secovallesamine moieties. Alkaloids 1 and 2 were obtained from the leaf extract while the bisindole 3 was isolated from the bark extract. In addition to the new …

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Study On The Supramolecular Interaction Of Inclision Complex Of Β - Cyclodextrin With Dithizone And Dithizone-Zinc And Its Analytical Application / Siti Nurur Raihan Mohd Kamal, Mohd Kamal Siti Nurur Raihan Jan 2011

Study On The Supramolecular Interaction Of Inclision Complex Of Β - Cyclodextrin With Dithizone And Dithizone-Zinc And Its Analytical Application / Siti Nurur Raihan Mohd Kamal, Mohd Kamal Siti Nurur Raihan

Student Works (2010-2019)

The non-covalent interaction of beta-cyclodextrin (β-CD), dithizone (H2Dz) and dithizone-zinc (H2Dz-Zn) complex was studied in detail. Solid samples were studied using solid state 13C Cross Polarization and Magic Angle Spinning spectroscopy (13C CP/MAS NMR), Fourier Transform Infrared spectroscopy (FTIR), Thermal Gravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Powder X-Ray Diffraction (XRD) and Energy Dispersive X-ray (EDX) to investigate the formation of the inclusion complex. Spectrophotometric technique was also used to assess the formation of inclusion complex in liquid form. The characterization results supported the formation of inclusion complex. The results showed that β-CD formed a complex with H2Dz in a …

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Conversion Of Spent Mushroom Sawdust Substrate To Simple Sugars For Bioethanol Production / Soo Ching Siew, Ching Siew Soo Jan 2011

Conversion Of Spent Mushroom Sawdust Substrate To Simple Sugars For Bioethanol Production / Soo Ching Siew, Ching Siew Soo

Student Works (2010-2019)

Spent Mushroom Sawdust Substrate (SMSS) was appraised for its potential as a lignocellulosic waste source for conversion of to bioethanol. Aqueous perchloric acid is used to hydrolyze the lignocellulosic waste feedstock to simple sugars which are then fermented to ethanol. Aqueous perchloric acid was chosen as the medium for conversion of SMSS to sugars as it can hydrolyze the recalcitrant cellulosic biomass to give a significant amount of glucose without the need for strong heating or the application of pressure. The products of the breakdown of SMSS by perchloric acid were analysed by HPLC, glucometer and by Fehling’s method. HPLC …

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Antioxidant Effects Of Rhodomyrtus Tomentosa (Kemunting) Extract On The Development Of Experimental Atherosclerotic-Induced New Zealand White Rabbit / Muhamad Fahrin Maskam, Muhamad Fahrin Maskam Jan 2011

Antioxidant Effects Of Rhodomyrtus Tomentosa (Kemunting) Extract On The Development Of Experimental Atherosclerotic-Induced New Zealand White Rabbit / Muhamad Fahrin Maskam, Muhamad Fahrin Maskam

Student Works (2010-2019)

The in vitro study was conducted to determine the antioxidant activity of R. tomentosa in water, methanol, chloroform and petroleum ether extract. In DPPH assay, methanol extract had the highest antioxidant capacity with IC50 of 107μg/ml. Antioxidant activity of R. tomentosa measured using FRAP assay also showed that methanol extract had the highest antioxidant capacity 0.162nm at concentration of 500μg/ml. While in Metal Chelating assay again showed methanol extract had the most chelating ability of all extracts with 36% at concentration of 100μg/ml. The chemical compounds found in R. tomentosa were quercetin, gallic acid and tannic acid from HPLC analyses; …

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Antioxidant Activity In Selected Local Vegetables / Sumathi A/P Murti, Sumathi Murti Jan 2011

Antioxidant Activity In Selected Local Vegetables / Sumathi A/P Murti, Sumathi Murti

Student Works (2010-2019)

A free radical is any atom or molecule that has a single unpaired electron in an outer shell and its damage is closely associated with oxidative damage. Oxidative damage by free radicals in the human body plays an important causative role in disease initiation and progression. Antioxidants provide a measure of protection that slows the process of oxidative damage. In this study, crude petroleum benzene, chloroform, methanol and water extracts of Allium tuberosum (garlic chives), Apium graveolens (L.) (celery), Ipomoea batatas (L.) (sweet potato leaves), Murraya koenigii (L.) (curry leaves), Psophocarpus tetragonolobus (winged bean), and Sauropus androgynus (sweet leaves) were …

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Screening And Partial Purification Of Protease Inhibitors From Senna Surattensis Leaves / Ahmad Firdhaus Arham, Ahmad Firdhaus Arham Jan 2011

Screening And Partial Purification Of Protease Inhibitors From Senna Surattensis Leaves / Ahmad Firdhaus Arham, Ahmad Firdhaus Arham

Student Works (2010-2019)

This study was done to determine the inhibitory activity that were exhibited by different parts of the local plants in Malaysia selected from the 4 families which are Leguminosae, Rubiaceae, Apocynaceae and Euphorbiaceae. The screening methods used in this study are Bradford Assay and Trypsin Inhibitory Assay. Both these assays revealed that Senna surattensis leaves showed the highest inhibitory activity of 83 % compared to other 41 plant samples studied in this study. SDS-PAGE and Tricine SDS on this sample extract showed the presence of this protease inhibitor through the formation of band. From these band also, the molecular weight …

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Mining And Validation Of Est-Microsatellites In Freshwater Prawns, Macrobrachium Rosenbergii / Shairah Abdul Razak, Shairah Abdul Razak Jan 2011

Mining And Validation Of Est-Microsatellites In Freshwater Prawns, Macrobrachium Rosenbergii / Shairah Abdul Razak, Shairah Abdul Razak

Student Works (2010-2019)

The current study conducted in purpose to illustrate the utility of EST-derived SSR in characterizing wild populations of M. rosenbergii in Malaysia‟s rivers. A novel set of EST-SSR generated from RNA of M. rosenbergii were validated in a full panel of 120 samples from four wild populations through Polymerase Chain Reaction (PCR). Seven EST-SSR loci were identified, characterized, and evaluated on 30 individuals each from the populations namely Negeri Sembilan, Kedah, Sarawak, and Terengganu. The average polymorphic informative content value (PIC) for these seven primers was found to be 0.6208 indicating considerable degree of polymorphism with number of alleles detected …

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Isolation And Purification Of Glutathione S-Transferases From Donax Sp / Norfarhan Mohd Assa’Ad, Norfarhan Mohd Assa’Ad Jan 2011

Isolation And Purification Of Glutathione S-Transferases From Donax Sp / Norfarhan Mohd Assa’Ad, Norfarhan Mohd Assa’Ad

Student Works (2010-2019)

Glutathione S-transferases (GSTs) are among the enzymes involved in the phase II detoxification metabolism of wide range exogenous and endogenous compounds in living cells. Bivalves GSTs often proposed as biomarker for marine pollution detection for several reasons; filter feeder, sessile, wide range distribution, and not affected by some biotic factors. In this study, GSTs from remis Donax sp. was purified by using two affinity column; GSTrapTMHP and GSH-agarose (C3). The total recovery of CDNB-active GSTs was 12% and 3% for GSTrapTMHP and GSH-agarose (C3), respectively. SDS-PAGE of GSTrapTMHP purified extract revealed two subunits with apparent molecular masses (MW) of 29 …

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Proteomic And Probit Analyses Of Glufosinate-Ammonium-Resistant Goosegrass(Eleusine Indica (L.) Gaertn.) Biotypes In Malaysia / Adam Bin Jalaludin, Adam Jalaludin Jan 2011

Proteomic And Probit Analyses Of Glufosinate-Ammonium-Resistant Goosegrass(Eleusine Indica (L.) Gaertn.) Biotypes In Malaysia / Adam Bin Jalaludin, Adam Jalaludin

Student Works (2010-2019)

Goosegrass (Eleusine indica [L.] Gaertn.), regarded as one of the world’s worst weeds is highly pernicious to cash crop growers in Malaysia. Following reports in 2009 that glufosinate ammonium failed to adequately control goosegrass populations in Kesang, Malacca and Jerantut, Pahang, Malaysia, on-site field trials were conducted to assess the efficacy of glufosinate-ammonium and glyphosate towards goosegrass from both places. Glufosinate-ammonium at 495 g ai ha1 managed to provide 82% control of the weed at the vegetable farm while the same rate failed to control goosegrass at the oil palm nursery. Glyphosate failed in controlling goosegrass population at both places …

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