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Articles 12841 - 12870 of 16826
Full-Text Articles in Entire DC Network
The Effects Of The Composition Of Growth Medium And Fermentation Conditions On The Production Of Lipase By R. Delemar, Ünsal Açikel, Mehtap Erşan, Yeşi̇m Sağ Açikel
The Effects Of The Composition Of Growth Medium And Fermentation Conditions On The Production Of Lipase By R. Delemar, Ünsal Açikel, Mehtap Erşan, Yeşi̇m Sağ Açikel
Turkish Journal of Biology
Lipases (triacylglycerol hydrolases) are hydrolytic enzymes that can catalyze the hydrolysis of the ester bond of long-chain acylglycerols at the oil-water interface. The present study investigated the effects of inducers, surface-active materials, activators, and inhibitors in the fermentation medium on lipase activity in Rhizopus delemar. In the presence of certain commercial oils and tributyrin as an inducer, lipase activity decreased in the order of sunflower oil > soybean oil > hazelnut oil > corn oil > tributyrin > olive oil. In addition, the effects of the stirring and aeration rates on lipase activity were investigated. To investigate the effect of surface-active materials on lipase activity …
Phylogenetic Analysis Of Peste Des Petits Ruminants Virus (Pprv) Isolated In Iran Based On Partial Sequence Data From The Fusion (F) Protein Gene, Majid Esmaelizad, Saber Jelokhani-Niaraki, Rohani Kargar-Moakhar
Phylogenetic Analysis Of Peste Des Petits Ruminants Virus (Pprv) Isolated In Iran Based On Partial Sequence Data From The Fusion (F) Protein Gene, Majid Esmaelizad, Saber Jelokhani-Niaraki, Rohani Kargar-Moakhar
Turkish Journal of Biology
In order to gain some insight into the origin of the peste des petits ruminants virus (PPRV) isolated in Iran, a selected region of the PPRV genome in a clinical sample collected from a sheep was amplified using RT-PCR, and the resulting amplicon was sequenced for phylogenetic analysis. The partial nucleotide and predicted amino acid sequence of the Iranian isolate was aligned with the corresponding sequences of 16 previously published F genes. Sequence analysis of the strains showed that the Iranian isolate had the highest degree of homology with the majority of the strains, with the exception of Nigerian isolates …
Effects Of Vesicular Arbuscular Mycorrhiza Glomus Intraradices On Photosynthetic Pigments, Antioxidant Enzymes, Lipid Peroxidation, And Chromium Accumulation In Maize Plants Treated With Chromium, Raziyeh Rahmaty, Jalil Khara
Effects Of Vesicular Arbuscular Mycorrhiza Glomus Intraradices On Photosynthetic Pigments, Antioxidant Enzymes, Lipid Peroxidation, And Chromium Accumulation In Maize Plants Treated With Chromium, Raziyeh Rahmaty, Jalil Khara
Turkish Journal of Biology
Contamination of soil and ground water by chromium (Cr), due to its wide industrial use, has become a serious source of concern over the past decade. In this study a glasshouse experiment was conducted to investigate the effects of the mycorrhizal fungus Glomus intraradices on Cr toxicity in maize plants. Half of the plants were inoculated with the arbuscular mycorrhizal fungus (AMF). Cr was supplied in the form of potassium dichromate at 0.00, 0.10, 0.25, and 0.50 mM through irrigation water in a sand culture. At the end of the experiment, it was observed that Cr had significantly decreased the …
The Effect Of Diazinon On Egg Fertility And Development In Drosophila Melanogaster, Ayla Karataş, Zafer Bahçeci̇, Ensar Başpinar
The Effect Of Diazinon On Egg Fertility And Development In Drosophila Melanogaster, Ayla Karataş, Zafer Bahçeci̇, Ensar Başpinar
Turkish Journal of Biology
This study investigated the effects of diazinon, an organic phosphoric insecticide, on some developmental features of Drosophila melanogaster. The aim was to determine if any developmental inhibition occurs when diazinon is applied and if so, to determine which developmental stages are affected. Therefore, diazinon was applied to Drosophila melanogaster eggs, larvae, and adults, and its effects were observed. During the experiments 3 concentrations with values lower than LC_{50} were utilized (6 × 10^{-5}, 6 × 10^{-6}, 6 × 10^{-7} ppm). Diazinon solution was applied to Drosophila melanogaster by means of nutrition, adding it to the culture medium. The results show …
Uv-B Induces Cell Death In The Lichen Physcia Semipinnata (J.F.Gmel), Di̇lek Ünal, Yi̇ği̇t Uyanikgi̇l
Uv-B Induces Cell Death In The Lichen Physcia Semipinnata (J.F.Gmel), Di̇lek Ünal, Yi̇ği̇t Uyanikgi̇l
Turkish Journal of Biology
We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses up to 95.9 J/cm2 was higher in the photobiont layer than the mycobiont layer.
The Toxicity Of A Synthetic Industrial Detergent And A Corrosion Inhibitor To Brackish Water Fish (Tilapia Guineensis), Dori̇s Fouwe Ogeleka, Lawrence Ikechukwu Ezemonye, Feli̇x E. Okieimen
The Toxicity Of A Synthetic Industrial Detergent And A Corrosion Inhibitor To Brackish Water Fish (Tilapia Guineensis), Dori̇s Fouwe Ogeleka, Lawrence Ikechukwu Ezemonye, Feli̇x E. Okieimen
Turkish Journal of Biology
The lethal effects resulting from incessant release of toxic surfactant-containing industrial chemicals into the Niger Delta Ecological Zone of Nigeria was studied using a laboratory toxicity test. The study employed the Organisation for Economic Development and Cooperation (OECD) 203 protocol. The test organism (Tilapia guineensis [fish]) was obtained from a brackish water environment and exposed to Neatex (industrial detergent) and Norust CR 486 (corrosion inhibitor) at concentrations of 6.25, 12.5, 25, 50, and 100 mg/L for 96 h. The ecological endpoint considered was mortality at 96 h, and the results obtained indicate that concentration and duration of exposure influenced the …
Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala
Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala
Turkish Journal of Biology
Detergent compatible alkaline protease production by a newly isolated Bacillus sp.Y from laundry soil was studied under shake flask conditions in growth medium comprised (g/L): peptone, 1; NaCl, 5; skim milk, 100; Na_2CO_3, 4. Different environmental process parameters such as fermentation period, initial pH, incubation temperature, and nutritional parameters such as supplementation of different starch sources and nitrogen sources were standardized for the maximum yield of alkaline protease. Rice flour showed an almost 2.2-fold increase in protease activity followed by wheat flour, which showed a 1.85-fold increase over the basal media. However, supplementation of potato starch, corn starch, and ragi …
An Entomopathogenic Bacterium, Pseudomonas Putida, From Leptinotarsa Decemlineata, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, Kazim Sezen
An Entomopathogenic Bacterium, Pseudomonas Putida, From Leptinotarsa Decemlineata, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, Kazim Sezen
Turkish Journal of Biology
The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was …
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
Turkish Journal of Biology
The efficacy of boric acid (BA) was examined on liver marker enzymes in aluminum (Al)-treated rats. Also, a liver micronucleus assay was performed to evaluate the genotoxicity in hepatocytes. With these aims, Sprague-Dawley rats were randomly separated into 10 groups of 5 animals. Aluminum chloride (5 mg/kg body weight (b.w.) AlCl3) and BA (3.25, 13, 36 and 58.5 mg/kg b.w.) alone were administered with injections to the experimental animals. Furthermore, the animals were also treated with Al for 4 consecutive days followed by BA exposure for 10 days. The rats were anesthetized after Al and BA injections and the levels …
Antagonistic Potential Of Marine Actinobacteria Against Fish And Shellfish Pathogens, Selvakumar Dharmaraj
Antagonistic Potential Of Marine Actinobacteria Against Fish And Shellfish Pathogens, Selvakumar Dharmaraj
Turkish Journal of Biology
A total of 94 marine actinobacterial isolates were found to be associated with 4 sponges: Callyspongia diffusa, Mycale mytilorum, Tedania anhelans, and Dysidea fragilis. Among the cultures 7 strains showed antagonism against fish and shellfish pathogens. Analysis of morphological, physiological, biochemical, and protein profile characteristics suggested that the actinobacterial strains belonged to the genus Streptomyces. The initial screening of the isolates by the spot inoculation method exhibited antibacterial activity against Aeromonas hydrophila. In vitro screening of the submerged culture extracts showed positive inhibition against the fish and shellfish pathogens Aeromonas hydrophila, Serratia sp., and Vibrio spp. The screening of bioactive …
Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz
Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz
Turkish Journal of Biology
In this study genetic variation was determined with isozyme analysis in 8 natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey, a part of the southern Palearctic region. For this purpose, 7 isozyme systems (ACP, AOX, CAT, HBDH, HXK, MDH, and SOD) were resolved using the native polyacrylamide gel electrophoresis (native-PAGE) technique. A total of 9 loci and 20 alleles were detected. The proportion of polymorphic loci in populations ranged from 44.44% to 66.67%. The overall mean number of effective alleles per locus (Ne) was 1.314 ± 0.454. The mean estimated expected heterozygosity (He) …
The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais
The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais
Turkish Journal of Biology
The analgesic effect of the ethanolic whole plant extract of Matricaria aurea L. (Asteraceae) was studied in rats using the cold water tail flick assay and in mice using the tail immersion, tail clip, acetic-acid-induced writhing, and formalin pain tests. The results showed dose-dependent and significant (P < 0.001) increases in pain threshold at 60 min post-treatment with doses of 100, 200, and 300 mg/kg of the extract in tail flick, tail immersion, and tail clip tests. The effects of the extract were significantly (P < 0.001) lower than those produced by morphine (10 mg/kg) in the same tests. The extract (100, 200, and 300 mg/kg) exhibited a dose-dependent inhibition of writhing and also showed a significant (P < 0.001) inhibition in both phases of the formalin pain test, but with a less intense effect in the first phase. The results indicate that the analgesic effect of M. aurea ethanolic extract is both centrally and peripherally mediated.
Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Turkish Journal of Biology
Ran binding proteins (RanBPs) are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated (Cucumis melo L.) using the RACE (rapid amplification of cDNA ends) method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids (22.2 kDa protein), was characterized (GenBank accession no: EU853459). The predicted amino acid sequence of CmRanBP was found to be 70% identical to VvRanBP, PtRanBP, and RcRanBP from Vitis vinifera, Populus trichocarpa, and Ricinus communis, respectively. …
The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç
The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç
Turkish Journal of Biology
: The present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic (90.52%), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei's genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be …
Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz
Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz
Turkish Journal of Biology
A series of studies were carried out to determine the succession and phytoplankton community of the Melen River in the Western Black Sea region of Turkey. The study was conducted at 6 stations at monthly intervals between May 2003 and April 2004. It was observed that the abundance and biovolume of phytoplankton were low in winter, though higher values were recorded in late spring and summer. Among the values noted, the spring peak was 534.38 × 10^5 ind. L^{-1} and 7384.8 mm^3 L^{-1} belonging to Peridinium sp. The most common diatom, Cyclostephanos dubius, was favoured by low discharge at stations …
Antibacterial Activity Of Bee Propolis Samples From Different Geographical Regions Of Turkey Against Two Foodborne Pathogens, Salmonella Enteritidis And Listeria Monocytogenes, Ayhan Temi̇z, Ayla Şener, Asli Özkök Tüylü, Kadri̇ye Sorkun, Beki̇r Sali̇h
Antibacterial Activity Of Bee Propolis Samples From Different Geographical Regions Of Turkey Against Two Foodborne Pathogens, Salmonella Enteritidis And Listeria Monocytogenes, Ayhan Temi̇z, Ayla Şener, Asli Özkök Tüylü, Kadri̇ye Sorkun, Beki̇r Sali̇h
Turkish Journal of Biology
The aim of the present study was to investigate the antibacterial activities of 25 propolis samples collected from various geographical regions of Turkey against 2 food-borne pathogens, Salmonella Enteritidis ATCC 13076 and Listeria monocytogenes ATCC 1462. The chemical compositions of ethyl alcohol extracts of the propolis (EEP) samples were determined by gas chromatography coupled to mass spectrometry. The main components of EEP samples were flavonoids, aromatic acid esters, aromatic alcohols, aromatic acids, aliphatic carboxylic acids, terpens, and aliphatic carboxylic acid esters. Antibacterial activities of the EEP samples were tested at 2 different dilutions of 1:10 and 1:100 (v/v). All EEP …
The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns
The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns
Turkish Journal of Biology
To evaluate possible interactions between the renin-angiotensin system (RAS) and the sympathetic nervous system (SNS) in the systemic circulation upon the intravenous administration of angiotensin II and a set of adrenergic agonists in normal Sprague-Dawley (SD) rats. Rats were treated with carvedilol, a non-selective \beta- and selective \alpha_1-adrenoceptor blocker, in a dose of (5 mg/kg) orally for 7 days, and on day 8, the animals were pentobarbitone-anaesthetized and prepared for systemic hemodynamic study. Dose-response relationships in terms of elevation in the magnitude of the mean arterial blood pressure in response to intravenous injection of noradrenaline (NA), phenylephrine (PE), methoxamine (ME), …
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Turkish Journal of Biology
This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and …
Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre
Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre
Turkish Journal of Biology
In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates …
Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan
Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan
Turkish Journal of Biology
The fruits of Carica papaya L. (Caricaceae) are valuable as food and are also used in traditional medicine. The present study was designed to assess the antioxidant potential of the juices of 3 papaya cultivars (PCJ): Sunrise Solo, Red Lady, and Tainung. The antioxidant capacity of PCJ obtained from fully ripened fruit was determined by the following methods: scavenging of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, scavenging of superoxide radicals, 2-deoxyribose oxidation assay, and thiocyanate assay. Total phenolic content (TPC) of PCJ was determined by the Folin-Ciocalteu method. In the DPPH assay (IC_{50} values), the effect of Sunrise …
Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak
Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak
Turkish Journal of Biology
The chemical composition and nutritional value of a wild edible ectomycorrhizal mushroom from southwestern Anatolia, Tricholoma anatolicum, were analyzed. Moisture, crude oil, protein, ash, total carbohydrate content, and mineral composition of the mushrooms studied, including Fe, Na, K, Zn, Cu, Ca, Cd, and Pb, were determined. The energy values of the samples were also calculated. The analyses were conducted during the 3 different growing stages of the mushrooms: mycelium, young fruiting bodies, and mature fruiting bodies. The highest values for moisture and crude oil contents were found to be in the mycelium, ash, and carbohydrate content of young fruiting bodies. …