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2010

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Hydraulic Conductivity, Infiltration, And Runoff From No-Till And Tilled Cropland, Jessica H. Deck Dec 2010

Hydraulic Conductivity, Infiltration, And Runoff From No-Till And Tilled Cropland, Jessica H. Deck

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Infiltration and runoff are important processes that affect the efficiency of center pivot irrigation systems. No-till planting systems potentially influence the hydraulic properties of soils and the soil surface conditions. The result of long-term use of no-till could be higher infiltration and lower runoff from rainfall and irrigation.

This potential was investigated in Nebraska on two center pivot irrigated sites; Fillmore County and Phelps County, one furrow irrigated site; South Central Agriculture Laboratory (SCAL), and one dryland site; Rogers Farm. Paired treatments were used at each location, one that was no-till planted and one that used two to three operations …


The In Situ Function Of A Microbial Community Profiled By Ft-Ir: A Snapshot In Time, Ryan Roberts Dec 2010

The In Situ Function Of A Microbial Community Profiled By Ft-Ir: A Snapshot In Time, Ryan Roberts

Department of Biochemistry: Dissertations, Theses, and Student Research

Photographs of an ecosystem are an important tool in macro-community ecology. The photograph is a permanent record of species phenotype. In microbiology, biochemical activity provides the most descriptive information of an organism’s phenotype. A method for fingerprinting all biochemical activities occurring within a microbial community is analogous to a photograph. Infrared spectroscopy in the region between wavelengths 2500 to 20,000 nm (mid-IR) is a well established instrumental method for fingerprinting the total biochemical profile of axenic cultures. Spectra are complex and sensitive as demonstrated by the ability to discriminate between strains of bacteria, fungi, and algae. This thesis develops the …


Environmental Conditions Of Green Valley Lake, Jennifer Bellamy Dec 2010

Environmental Conditions Of Green Valley Lake, Jennifer Bellamy

All-Inclusive List of Electronic Theses and Dissertations

Green Valley Lake, western Indiana, has experienced periodic inputs of acid mine drainage (AMD) from the abandoned Green Valley Coal Mine. The lake serves as a state fishing area, and AMD inputs may affect the aquatic ecosystem and human health. The purpose of this research is to determine to what extent the sediments in Green Valley Lake are acting as sinks for metals and if they may impact water quality. Water and bottom sediment samples were taken throughout the lake to evaluate spatial variability of contamination and to determine how the metal concentrations compared to Post Archean Average Shale (PAAS) …


The Status Of Crawfish Frogs (Lithobates Areolatus) In Indiana, And A Tool To Assess Populations, Nathan J. Engbrecht Dec 2010

The Status Of Crawfish Frogs (Lithobates Areolatus) In Indiana, And A Tool To Assess Populations, Nathan J. Engbrecht

All-Inclusive List of Electronic Theses and Dissertations

The conservation status of Crawfish Frogs (Lithobates areolatus) in Indiana has changed over the past several decades. Once described as being locally plentiful, declines led to the listing of Crawfish Frogs as a State Endangered Species in 1988. Several records for this species in Indiana are > 50 yrs old and have gone unconfirmed for several decades. However, recent surveys have confirmed the continued presence of Crawfish Frogs in parts of southern Indiana, redefining the perceived range of this species in the state. In an effort to increase survey efficiency in this species, I used automated recording systems and manual call …


Counterintuitive Effects Of Large-Scale Predator Removal On A Midlatitude Rodent Community, John L. Maron, Dean E. Pearson, Robert J. Fletcher Jr. Dec 2010

Counterintuitive Effects Of Large-Scale Predator Removal On A Midlatitude Rodent Community, John L. Maron, Dean E. Pearson, Robert J. Fletcher Jr.

Biological Sciences Faculty Publications

Historically, small mammals have been focal organisms for studying predator–prey dynamics, principally because of interest in explaining the drivers of the cyclical dynamics exhibited by northern vole, lemming, and hare populations. However, many small-mammal species occur at relatively low and fairly stable densities at temperate latitudes, and our understanding of how complex predator assemblages influence the abundance and dynamics of these species is surprisingly limited. In an intact grassland ecosystem in western Montana, USA, we examined the abundance and dynamics of Columbian ground squirrels (Spermophilus columbianus), deer mice (Peromyscus maniculatus), and montane voles (Microtus montanus …


Comparison Of Pcr And Rt-Pcr In The First Report Of Batrachochytrium Dendrobatidis In Amphibians In New Jersey, Usa, Kirsten Monsen, Lisa Hazard, Rakesh Dussa Dec 2010

Comparison Of Pcr And Rt-Pcr In The First Report Of Batrachochytrium Dendrobatidis In Amphibians In New Jersey, Usa, Kirsten Monsen, Lisa Hazard, Rakesh Dussa

Department of Biology Faculty Scholarship and Creative Works

No abstract provided.


Curbing Energy Sprawl With Microgrids, Sara C. Bronin Dec 2010

Curbing Energy Sprawl With Microgrids, Sara C. Bronin

Cornell Law Faculty Publications

Energy sprawl - the phenomenon of ever-increasing consumption of land, particularly in rural areas, required to site energy generation facilities - is a real and growing problem. Over the next twenty years, at least sixty-seven million acres of land will have been developed for energy projects, destroying wildlife habitats and fragmenting landscapes. According to one influential report, even renewable energy projects - especially large-scale projects that require large-scale transmission and distribution infrastructure - contribute to energy sprawl. This Article does not aim to stop large-scale renewable energy projects or even argue that policymakers focus solely on land use in determining …


Polymer (Su-8) Optofluidic Device With Embedded Hydrogel Oxygen Sensing Elements, Zhan Gao, David B. Henthorn, Chang-Soo Kim Dec 2010

Polymer (Su-8) Optofluidic Device With Embedded Hydrogel Oxygen Sensing Elements, Zhan Gao, David B. Henthorn, Chang-Soo Kim

Chemical and Biochemical Engineering Faculty Research & Creative Works

The SU-8 photopolymer is an excellent alternative for traditional materials used in fluidic devices such as glass- or silicon-Based inorganic materials. We describe a new chemically anchored, lithographically patterned hydrogel sensing element embedded in a completed SU-8 fluidic channel structure after the channel bonding process. Polyethylene glycol (PEG) hydrogel films incorporating oxygen-responsive ruthenium-complex fluorophores are grafted and patterned by photopolymerization of solution-phase precursors on the modified SU-8 channel surface. the optofluidic sensing platform with this embedded sensing elements exhibited excellent performance in measuring dissolved oxygen content. a unique advantage of this method is the selective growth of various functional films …


The Impact Of Long-Term Tillage, Crop Rotation And N Application On Soil Carbon Sequestration, Agustin Martellotto Dec 2010

The Impact Of Long-Term Tillage, Crop Rotation And N Application On Soil Carbon Sequestration, Agustin Martellotto

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Soil organic carbon (SOC) stock is controlled by many factors, but for given conditions it is the long term summation of the balance between inputs and outputs. Management practices will alter this balance by affecting the system’s productivity and the speed of residue and soil organic matter decomposition. Given that annual changes in SOC are generally small, compared with the large and variable SOC background, long-term experiments, and soil samples taken at the start of experiments (archived samples) are necessary to determine SOC trends over time. Changes in SOC were analyzed for two long-term experiments; one an irrigated site at …


Twenty-Eight Years Of The Us-Lter Program: Experience, Results, And Research Questions, James R. Gosz, Robert B. Waide, John J. Magnuson Dec 2010

Twenty-Eight Years Of The Us-Lter Program: Experience, Results, And Research Questions, James R. Gosz, Robert B. Waide, John J. Magnuson

Long Term Ecological Research Network

The U.S. Long Term Ecological Research program (hereafter US-LTER) concentrates on ecological processes that play out at the time scales spanning decades to centuries. This focuses US-LTER research between the most common time scales for ecological studies (1-3 years; Tilman 1989; Figure 1) and the much longer temporal fact of disciplines such as paleoecology. The importance of the decade-to-century time scale is particularly evident in light of the rapid changes in ecological forcing functions that are occurring at a broad range of spatial scales (Millennium Ecosystem Assessment 2005, Intergovernmental Panel on Climate Change 2007). Long-term data sets from programs such …


Delaying Bud Break In ‘Edelweiss’ Grapevines To Avoid Spring Frost Injury By Naa And Vegetable Oil Applications, Issam M. Qrunfleh Dec 2010

Delaying Bud Break In ‘Edelweiss’ Grapevines To Avoid Spring Frost Injury By Naa And Vegetable Oil Applications, Issam M. Qrunfleh

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Delaying bud break is an approach to avoid spring frost damage. Field experiments were conducted during the winters of 2009 and 2010 at James Arthur Vineyards in Raymond, Nebraska to study the effect of spraying NAA and Amigo Oil on delaying bud break in ‘Edelweiss’ grapevines to avoid such damage. In 2009, the experiment consisted of five treatments: NAA (500, 750, and 1,000 mg/l), oil applied at 10%, and the non-sprayed control. There were four application dates: January 6, February 3, March 3, and April 1. Bud break was evaluated throughout spring. During harvest, the number of clusters and weights …


A Case Study Of The Mathematical Learning Of Two Teachers Acquiring Mathematical Knowledge For Teaching, David R. Hartman Dec 2010

A Case Study Of The Mathematical Learning Of Two Teachers Acquiring Mathematical Knowledge For Teaching, David R. Hartman

College of Education and Human Sciences: Dissertations, Theses, and Student Research

This study offers an analysis of the learning of practicing teachers as they acquire a deeper knowledge of mathematics. While some professional developers have shifted part of their focus to helping practicing teachers acquire a deeper knowledge of mathematics (e.g., Stein & Silver, 1996), the results from studies often describe what translates from the professional development experience into classroom practice and measureable gains in student achievement (e.g., Desimone et al., 2002). Studies showing improvements in pedagogy and student learning are important. However, studying what teachers are learning and how they learn is important in developing understanding of the content and …


The Role Of Peroxidase In The Defense Response Of Buffalograss To Chinch Bugs, Anh Hoang Dec 2010

The Role Of Peroxidase In The Defense Response Of Buffalograss To Chinch Bugs, Anh Hoang

Department of Entomology: Dissertations, Theses, and Student Research

In recent years, buffalograss, Buchloë dactyloides (Nuttall) Engelmann has gained popularity as a turfgrass because of its low maintenance requirement, drought tolerance, and limited pests and diseases. Within the last decade, however, the western chinch bug Blissus occiduus Barber has emerged as important pest of buffalograss. Considerable progress has been made toward identifying buffalograsses with resistance to the western chinch bug and understanding the mechanisms of the resistance. It has been hypothesized that chinch bug-resistant buffalograsses can effectively detoxify the elevated levels of Reactive Oxygen Species (ROS) through enhanced activity of ROS-scavenging enzymes, while ROS accumulates in the susceptible plants …


Examination Of Egg White Proteins And Effects Of High Pressure On Select Physical And Functional Properties, Andrew Hoppe Dec 2010

Examination Of Egg White Proteins And Effects Of High Pressure On Select Physical And Functional Properties, Andrew Hoppe

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Egg white proteins have become an important and desirable ingredient to the food industry due to their functional properties which include gelling, foaming, and emulsification. Egg white is also well recognized as an excellent source of nutrition. The goal of this work was to determine the effects of high pressure (HP) treatment on egg white proteins. Specifically, experiments were conducted using Raman spectroscopy and pepsin digestibility to investigate structural changes. Pressure treatment at 400 to 800 MPa (5 minutes at 4°C) resulted in increased pepsin digestibility of egg white proteins ovalbumin, ovotransferrin, and lysozyme compared to heat-treated (85 to 95°C) …


Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh Dec 2010

Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Holocarboxylase synthetase (HCS) is a chromatin protein that is essential for mediating the covalent binding of biotin to histones. Biotinylation of histones plays crucial roles in the repression of genes and repeats in the human genome. The objective of this project was to determine genomic binding sites of holocarboxylase synthetase (HCS) in the human chromatin of breast cell lines using an antibody independent technology. We tested the feasibility of DNA adenine methyltransferase identification (DamID) technology to map HCS binding sites in human mammary cell lines. Full-length HCS was fused to Dam for subsequent transfection into breast cancer (MCF-7) and normal …


Shovelnose Sturgeon Age And Growth Characteristics And Fish Community Characteristics Of The Lower Platte River And Missouri River Near Nebraska, Tara L. Anderson Dec 2010

Shovelnose Sturgeon Age And Growth Characteristics And Fish Community Characteristics Of The Lower Platte River And Missouri River Near Nebraska, Tara L. Anderson

School of Natural Resources: Dissertations, Theses, and Student Research

There have been documented widespread losses of freshwater biodiversity over the past several decades. Many of these losses may be due to anthropogenic causes such as habitat alterations, pollution, and overharvest. Shovelnose sturgeon may also be influenced by anthropogenic causes and have experienced declines in population abundance near Nebraska. My objectives were to first to describe the age, growth and mortality of shovelnose sturgeon found in the lower Platte River (LPR). Second, I quantitatively evaluated similarities and differences between the Missouri River (MR) and the LPR fish community composition (presence-absence of species) and structure (abundance of species). We used trammel …


The Interaction Of Fire And Grazing In Oklahoma Artemisia Filifolia Shrubland, Stephen L. Winter Dec 2010

The Interaction Of Fire And Grazing In Oklahoma Artemisia Filifolia Shrubland, Stephen L. Winter

School of Natural Resources: Dissertations, Theses, and Student Research

I. FIRE-RESILIENCY OF A NORTH AMERICAN ARTEMISIA (ASTERACEAE) SHRUB: IMPLICATIONS FOR RESTORATION OF A CRITICAL ECOSYSTEM PROCESS. Conclusions: Unlike most North American Artemisia shrub species, our research suggests that A. filifolia is highly resilient to the effects of fire. Therefore, use of prescribed fires for the restoration and maintenance of ecosystem processes and properties is appropriate in A. filifolia shrublands of the southern Great Plains in North America.

II. RESTORATION OF THE FIRE-GRAZING INTERACTION IN ARTEMISIA FILIFOLIA SHRUBLAND OF THE SOUTHERN GREAT PLAINS, NORTH AMERICA. Synthesis and applications. Vegetation structure in Artemisia filifolia shrublands of our study were readily altered …


Micropropagation And Acclimatization Of 'Norton' Grapevine (Vitis Aestivalis), Brant B. Bigger Dec 2010

Micropropagation And Acclimatization Of 'Norton' Grapevine (Vitis Aestivalis), Brant B. Bigger

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Propagation of the Vitis aestivalis cultivar 'Norton‘ (syn='Cynthiana‘) through traditional woody cuttings has been difficult. Rooting of woody cuttings has been a major hindrance in propagating this cultivar and providing enough plants to meet grower needs. In vitro propagation offers another method of increasing plant material. Cultures were established and maintained on Murashige and Skoog (MS) medium supplemented with 4 M 6-benzyladenine (BA) and thiamine at 0.5 mg•L-1 and solidified with Difco-Bacto agar at 7.5 g•L-1. The objectives of this study were to determine optimal methods for in vitro production and ex vitro establishment of 'Norton‘ plantlets. …


Ultrasonic Activation Of Triacetone Triperoxide, Latravia R. Dobson Dec 2010

Ultrasonic Activation Of Triacetone Triperoxide, Latravia R. Dobson

Department of Chemistry: Dissertations, Theses, and Student Research

Triacetone triperoxide (TATP) is an organic peroxide that has received widespread attention in recent years. TATP is a ketone peroxide with a high active oxygen content. It is relatively shock sensitive, with explosive decomposition easily initiated, and is therefore considered a primary explosive. However, TATP is also a powerful explosive possessing about 83 % of the power of TNT. TATP can be prepared quickly and easily from inexpensive household chemicals in the absence of any specialized facilities, making it the explosive of choice for modern day terrorists. TATP poses a major challenge for security and law enforcement services around the …


I, A New Synthetic Approach To The Synthesis Of N-(Phosphonoacetyl)-L-Ornithine. Ii, The Influence Of Pyridine On The Ozonolysis Of Alkenes, Bradley M. Johnson Dec 2010

I, A New Synthetic Approach To The Synthesis Of N-(Phosphonoacetyl)-L-Ornithine. Ii, The Influence Of Pyridine On The Ozonolysis Of Alkenes, Bradley M. Johnson

Department of Chemistry: Dissertations, Theses, and Student Research

Part I. The use of chemical inhibitors to manipulate the level of amino acids in cells has proven to be invaluable in the mechanistic study of gene expression in bacteria and fungi. Here we present a new approach to the synthesis of δ‐N‐ (phosphonoacetyl)‐L‐ornithine (PALO), a potent ornithine transcarbamylase inhibitor, using a new amino acid protecting group, 9‐borabicyclononane (9‐BBN). Starting from commercially available reagents and utilizing mild reaction conditions, we were able to form PALO in fewer synthetic steps and in greater yields than previous attempts.

Part II. Ozonolysis is widely used to transform alkenes into oxygen‐rich functional groups (e.g.– …


Application Of Homotopy Perturbation Method To Biological Population Model, Pradip Roul Dec 2010

Application Of Homotopy Perturbation Method To Biological Population Model, Pradip Roul

Applications and Applied Mathematics: An International Journal (AAM)

In this article, a well-known analytical approximation method, so-called the Homotopy perturbation method (HPM) is adopted for solving the nonlinear partial differential equations arising in the spatial diffusion of biological populations. The resulting solutions are compared with those of the existing solutions obtained by employing the Adomian’s decomposition method. The comparison reveals that our approximate solutions are in very good agreement with the solutions by Adomian’s method. Moreover, the results show that the proposed method is a more reliable, efficient and convenient one for solving the non-linear differential equations.


Two-Layered Pulsatile Blood Flow In A Stenosed Artery With Body Acceleration And Slip At Wall, Devajyoti Biswas, Uday Shankar Chakraborty Dec 2010

Two-Layered Pulsatile Blood Flow In A Stenosed Artery With Body Acceleration And Slip At Wall, Devajyoti Biswas, Uday Shankar Chakraborty

Applications and Applied Mathematics: An International Journal (AAM)

Pulsatile flow of blood through an artery in presence of a mild stenosis has been investigated in this paper assuming the body fluid blood as a two-fluid model with the suspension of all the erythrocytes in the core region as Bingham Plastic and the peripheral region of plasma as a Newtonian fluid. This model has been used to study the influence of body acceleration, non- Newtonian nature of blood and a velocity slip at wall, in blood flow through stenosed arteries. By employing a perturbation analysis, analytic expressions for the velocity profile, Plug-core radius, flow rate, wall shear stress and …


Expression And Localization Of An Hsp70 Protein In The Microsporidian Encephalitozoon Cuniculi, Carrie E. Jolly, Cory A. Leonard, James R. Hayman Dec 2010

Expression And Localization Of An Hsp70 Protein In The Microsporidian Encephalitozoon Cuniculi, Carrie E. Jolly, Cory A. Leonard, James R. Hayman

ETSU Faculty Works

Microsporidia spore surface proteins are an important, under investigated aspect of spore/host cell attachment and infection. For comparison analysis of surface proteins, we required an antibody control specific for an intracellular protein. An endoplasmic reticulum-associated heat shock protein 70 family member (Hsp70; ECU02 0100; "C1") was chosen for further analysis. DNA encoding the C1 hsp70 was amplified, cloned and used to heterologously express the C1 Hsp70 protein, and specific antiserumwas generated. Two-dimensional Western blotting analysis showed that the purified antibodies were monospecific. Immunoelectron microscopy of developing and mature E. cuniculi spores revealed that the protein localized to internal structures and …


Zoledronic Acid: Clinical Utility And Patient Considerations In Osteoporosis And Low Bone Mass, Ronald C. Hamdy Dec 2010

Zoledronic Acid: Clinical Utility And Patient Considerations In Osteoporosis And Low Bone Mass, Ronald C. Hamdy

ETSU Faculty Works

The availability of a once-a-year zoledronic acid infusion heralds a new era in the management of osteoporosis. It virtually eliminates the problem of poor compliance with orally administered bisphosphonates and, because it bypasses the gastrointestinal tract, it is not associated with gastrointestinal side effects. Zoledronic acid is effective for the treatment and prevention of postmenopausal osteoporosis, and for the treatment of osteoporosis in men, and glucocorticoid- induced osteoporosis. When administered within three months of a hip fracture, it reduces mortality and the risk of subsequent fractures. It is remarkably free of serious adverse effects. After administration of the intravenous infusion, …


New Fat Free Mass - Fat Mass Model For Use In Physiological Energy Balance Equations, Diana Thomas, Sai Das, James A. Levine, Corby K. Martin, Laurel Mayer, Andrew Mcdougall, Boyd J. Strauss, Steven B. Heymsfield Dec 2010

New Fat Free Mass - Fat Mass Model For Use In Physiological Energy Balance Equations, Diana Thomas, Sai Das, James A. Levine, Corby K. Martin, Laurel Mayer, Andrew Mcdougall, Boyd J. Strauss, Steven B. Heymsfield

Department of Mathematics Faculty Scholarship and Creative Works

Background: The Forbes equation relating fat-free mass (FFM) to fat mass (FM) has been used to predict longitudinal changes in FFM during weight change but has important limitations when paired with a one dimensional energy balance differential equation. Direct use of the Forbes model within a one dimensional energy balance differential equation requires calibration of a translate parameter for the specific population under study. Comparison of translates to a representative sample of the US population indicate that this parameter is a reflection of age, height, race and gender effects. Results: We developed a class of fourth order polynomial equations relating …


Ambiguity And Morality In Jelinek's Bambiland, Andrea Bandhauer Dec 2010

Ambiguity And Morality In Jelinek's Bambiland, Andrea Bandhauer

CLCWeb: Comparative Literature and Culture

In her article "Ambiguity and Morality in Jelinek's Bambiland" Andrea Bandhauer begins by noting that the language of Elfriede Jelinek's play Bambiland (2004) is characterized by experimentation and a propensity for complex and ambiguous word plays. In this play, her critique of the media is directed against the international, or rather, Western press and its role in the Iraq war. The text of Bambiland, conceptualized as a "work-in-progress," in which Jelinek posed as an "embedded writer," started to appear on her website at the beginning of the war and Jelinek continued writing it through 2003. In the text of the …


String Theory And Our Relationship With Nature: The Convergence Of Science, Curriculum Theory, And The Environment, Virginia Therese Bennett Dec 2010

String Theory And Our Relationship With Nature: The Convergence Of Science, Curriculum Theory, And The Environment, Virginia Therese Bennett

College of Graduate Studies: Theses & Dissertations

Curriculum Theory affords us the opportunity to examine education from a multitude of directions. This work takes advantage of that opportunity to explore the relationships between science, nature, and curriculum using string theory and our ideas about the environment as a backdrop. Both the energy and multiple possibilities created by strings and the rich history leading up to the theory help to illustrate the many opportunities we have to advance discussions in alternative ways of looking at science. By considering the multiple dimensions inherent in string theory as multiple pathways and interweaving metaphors from Deleuze and Guattari, Michel Serres, and …


Administrators' And Teachers' Perceptions Of In-School Suspension, Amelia Shondra Jackson Dec 2010

Administrators' And Teachers' Perceptions Of In-School Suspension, Amelia Shondra Jackson

Dissertations

Providing a safe and orderly school environment for students to learn is of paramount importance to school administrators, teachers, students, and the general public; however, the learning process should not be compromised while trying to maintain such an environment. There are many alternative approaches that can be utilized that would rectify the problem we as educators face in providing a safe and orderly environment. At-risk students are usually the ones who have the most serious infractions. These students exhibit a cyclical pattern of recalcitrant behavior. It is imperative that these at-risk students be identified as early as possible, and intervention …


Panorama December 2010, Southern Adventist University Dec 2010

Panorama December 2010, Southern Adventist University

Panorama Parent Newsletter

This issue of the Panorama parent newsletter provides information about outdoor adventure locations in the area, contains a profile on Professor Marty Miller, and highlights the SOAP program.


Age-Associated Changes In Synaptic Lipid Raft Proteins Revealed By Two-Dimensional Fluorescence Difference Gel Electrophoresis, Lei Jiang, Jianwen Fang, David S. Moore, Natalia V. Gogichaeva, Nadezhda A. Galeva, Mary L. Michaelis, Asma Zaidi Dec 2010

Age-Associated Changes In Synaptic Lipid Raft Proteins Revealed By Two-Dimensional Fluorescence Difference Gel Electrophoresis, Lei Jiang, Jianwen Fang, David S. Moore, Natalia V. Gogichaeva, Nadezhda A. Galeva, Mary L. Michaelis, Asma Zaidi

Faculty and Staff Publications

Brain aging is associated with a progressive decline in cognitive function though the molecular mechanisms remain unknown. Functional changes in brain neurons could be due to age-related alterations in levels of specific proteins critical for information processing. Specialized membrane microdomains known as 'lipid rafts' contain protein complexes involved in many signal transduction processes. This study was undertaken to determine if two-dimensional fluorescence difference gel electrophoresis (2D DIGE) analysis of proteins in synaptic membrane lipid rafts revealed age-dependent alterations in levels of raft proteins. Five pairs of young and aged rat synaptic membrane rafts were subjected to DIGE separation, followed by …