Digital Commons Network^™

Immunophenotypic Profile Of Acute Leukemias At Agakhan University Hospital, Nairobi, Beatrice M. Kabera

Master of Medicine in Clinical Pathology

Background: Reliable distinction between AML and ALL is important for the selection of appropriate therapy. The final diagnosis of acute leukemia should be based on clinico-pathological, morphological, cytochemical and molecular cytogenetics data, together with immunophenotypic information. Flow cytometry has become the preferred method for the lineage assignment and maturational analysis of malignant cells in acute leukemias. Immunophenotyping has also allowed the detection of aberrant antigen co expression and the analysis of heterogeneity and clonality of malignant cells in acute leukemias. It is insufficient to merely make a diagnosis of acute leukemia, or even AML or ALL. Further classification is essential …

Summer Commencement Ceremonies, University Of North Florida

Commencement Printed Materials

Program for the Summer Commencement ceremonies.

Peroxisomal Plant 3-Ketoacyl-Coa Thiolase Structure And Activity Are Regulated By A Sensitive Redox Switch, Valerie E. Pye, Caspar E. Christensen, Jim Dyer, Susan Arent, Anette Henriksen

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

The breakdown of fatty acids, performed by the β-oxidation cycle, is crucial for plant germination and sustainability. β-Oxidation involves four enzymatic reactions. The final step, in which a two-carbon unit is cleaved from the fatty acid, is performed by a 3-ketoacyl-CoA thiolase (KAT). The shortened fatty acid may then pass through the cycle again (until reaching acetoacetyl-CoA) or be directed to a different cellular function. Crystal structures of KAT from Arabidopsis thaliana and Helianthus annuus have been solved to 1.5 and 1.8 Å resolution, respectively. Their dimeric structures are very similar and exhibit a typical thiolase-like fold; dimer formation and …

Assessing Economic And Hrql Burden Of Food Allergy And Anaphylaxis In The U.S., Dipen Patel

Theses and Dissertations

Background: Food allergy, an abnormal immunologic response to food protein, has an estimated prevalence of 6% in young children and 3.7% in adults in the U.S. The only proven therapy for food allergy is strict elimination of the offending allergens. As a result, caregivers and patients could experience constant anxiety and stress that affects their quality of life. Additionally, food allergy can lead to significant economic impact on the health care system, since severe reactions often lead to ED visits and hospitalizations. Objectives: The first major objective was to determine the economic burden of Food Allergy and Anaphylaxis (FAA) patients …

Contribution Of Nuceliec Acids On The Structure Of Recombinant Hepadnavirus Core Antigens, Maimuna Bruce

Theses and Dissertations

The Hepatitis B core antigen (HBcAg) has been proposed to be an ideal candidate for use as an adjuvant due to its immunogenicity, and tolerance to manipulations such as insertions of epitopes or covalent attachment of ligands. HBcAg is a complex macromolecule containing protein and nucleic acid. We investigated the effect of the removal and reconstitution of nucleic acids upon its structure. It’s been shown that the RNA content of hepadnavirus core antigens can be reduced significantly, but not be completely removed. Following removal of some of the RNA, antigens retain the ability to bind added nucleic acids, in particular, …

Structural Characterization Of Hiv Gp41 With The Membrane-Proximal External Region, Wuxian Shi, Jen Bohon, Mark R. Chance

Faculty Scholarship

Human immunodeficiency virus, type 1 (HIV-1) envelope glycoprotein (gp120/gp41) plays a critical role in virus infection and pathogenesis. Three of the six monoclonal antibodies considered to have broadly neutralizing activities (2F5, 4E10, and Z13e1) bind to the membrane-proximal external region (MPER) of gp41. This makes the MPER a desirable template for developing immunogens that can elicit antibodies with properties similar to these monoclonal antibodies, with a long term goal of developing antigens that could serve as novel HIV vaccines. In order to provide a structural basis for rational antigen design, an MPER construct, HR1-54Q, was generated for x-ray crystallographic and …

The Effects Of A Polynutrient Dietary Supplement On Physiological Measures And Mood State In Resistance Trained Men, Thomas Incledon

Open Access Dissertations

The purpose of the present study was to test the acute effects of a dietary supplement, having as its major ingredient an extract of ginseng, on grip strength, lower body power output, cardiovascular markers, metabolic markers, hormones, and mood state. Twelve experienced resistance-trained men (28.3 ± 5.7 yrs) were randomly administered placebo (P), single dose (SD) and double dose (DD) of the supplement on separate days. Diet and activity levels were kept constant across testing days. On each day, subjects began with the Profile of Mood States (POMSpre1), blood draws (BDpre1), blood pressure (BPpre1), and heart rate (HRpre1) assessments, then …

Nsf Grant To Fund August Conference For Minority Faculty, Winthrop University

Winthrop News 2010

• The conference will be held Aug 12-14 in Chantilly, Va.
• Attending the conference will be 50 junior and senior faculty members from public and private institutions who have been awarded National Science Foundation grants.

07-29-2010 Swosu Announces Summer Graduates, Southwestern Oklahoma State University

THE BARK ARCHIVE 2010

A total of 155 students at Southwestern Oklahoma State University completed requirements for either associate, bachelor's, master's or doctoral degrees this summer on the Weatherford and Sayre campuses.

Preservation Ethics In The Case Of Nebraska's Nationally Registered Historic Properties, Darren Michael Adams

Department of Geography: Dissertations, Theses, and Student Research

This dissertation focuses on the National Register of Historic Places and considers the geographical implications of valuing particular historic sites over others. Certain historical sites will either gain or lose desirability from one era to the next, this dissertation identifies and explains three unique preservation ethical eras, and it maps the sites which were selected during those eras. These eras are the Settlement Era (1966–1975), the Commercial Architecture Era (1976–1991), and the Progressive Planning Era (1992–2010). The findings show that transformations in the program included an early phase when state authorities listed historical resources pertaining to the settlement of the …

Meditation Acutely Improves Psychomotor Vigilance, And May Decrease Sleep Need, Prashant Kaul, Jason Passafiume, Craig R. Sargent, Bruce F. O'Hara

Biology Faculty Publications

BACKGROUND: A number of benefits from meditation have been claimed by those who practice various traditions, but few have been well tested in scientifically controlled studies. Among these claims are improved performance and decreased sleep need. Therefore, in these studies we assess whether meditation leads to an immediate performance improvement on a well validated psychomotor vigilance task (PVT), and second, whether longer bouts of meditation may alter sleep need.

METHODS: The primary study assessed PVT reaction times before and after 40 minute periods of mediation, nap, or a control activity using a within subject cross-over design. This study utilized novice …

Essays On Adolescent Behavior And Outcomes: Influence Of Parents, Peers, Schools, And Neighborhoods, Debjani Kanjilal

Electronic Theses and Dissertations Archive

Adolescence is an important phase in one’s life since it shapes up one’s future in terms of academic, economic, behavioral, and health related outcomes, among others. The transition period between childhood and adulthood has been believed to involve major physical, social, and psychological changes which can affect future outcomes of an individual. Parental decisions are indeed important for subsequent behavior of adolescents which may also persist over time. Apart from parents, social network groups also play an important role in an adolescent’s life. Social groups may include friends, schools, neighborhoods, cultural groups, etc. and there is evidence of significant effects …

Poxa, Yjek And Elongation Factor P Coordinately Modulate Virulence And Drug Resistance In Salmonella Enterica, William Wiley Navarre, Shicong Zou, Hervé Roy, Jinglin Lucy Xie, Alexei Savchenko, Alexander Singer, Elena Edvokimova, Lynne R. Prost, Runjun Kumar, Michael Ibba, Ferric C. Fang

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-β-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with …

Semester Of Service

News Releases

No abstract provided.

Differential Toxicity Of Dna Adducts Of Mitomycin C, Jill Bargonetti, Elise Champeil, Maria Tomasz

Publications and Research

The clinically used antitumor agent mitomycin C (MC) alkylates DNA upon reductive activation, forming six covalent DNA adducts in this process. This review focuses on differential biological effects of individual adducts in various mammalian cell cultures, observed in the authors' laboratories. Evidence is reviewed that various adducts are capable of inducing different cell death pathways in cancer cells.This evidence is derived from a parallel study of MC and its derivatives 2,7-diaminomitosene (2,7-DAM) which is the main metabolite of MC and forms two mono-adducts with DNA, and decarbamoyl mitomycin C (DMC), which alkylates and cross-links DNA, predominantly with a chirality opposite …

Function And Regulation Of Matrix Metalloproteinase-1 In Glioblastoma Multiforme, Monika Anand

Theses and Dissertations

Glioblastoma Multiforme (GBM) is an aggressive and fatal cancer of the brain. It is characterized with augmented morbidity and elusion to therapies due in part to the incessant infiltration and spread of tumor cells in normal brain. We investigated the function of Matrix metalloproteinase-1, an important enzyme noted to be responsible for invasion in other cancers, in GBM and its regulation by epidermal growth factor receptor (EGFR) signaling. Previous studies from our laboratory demonstrated elevated levels of MMP-1 in GBM. Further studies indicated the involvement of MMP-1 in GBM invasion. The GBM cell lines T98G, U251MG and U87MG were used …

Characterization And Crystallization Of The Mycobacterium Tuberculosis Trmd, Zohal Hamidi

Theses and Dissertations

One third of the world’s population is affected by Tuberculosis (TB), a disease caused by infection with Mycobacterium tuberculosis (MtB). The emergence of multidrug-resistant MtB makes this disease a major public health concern. New agents are needed to treat TB infections in a manner that circumvents existing pathways of resistance. One strategy is to target the organism at the translational level by inhibiting vital modifications of RNA. One gene responsible for these modifications is the tRNA (guanosine-1)-methyltransferase, trmD, which has been shown to be essential in several bacteria. The eukaryotic and bacterial m1G methyltransferases are structurally dissimilar, making this enzyme …

A New Species Of Calceolaria (Calceolariaceae) From Disturbed Paramos In South Ecuador, Pamela Puppo

Biological Sciences Faculty Research

Calceolaria molaui Puppo, sp. nov. is described and illustrated. The new species was collected in Loja, in disturbed paramos near the frontier with Peru so it would be expected to occur in northern Peru as well. It is characteristic for having hirsute indumentum composed of branched trichomes, ovate, subcoriaceous leaves with reticulate venation, cyme bracts absent, elaiophore absent, and brown, deflexed anthers, totally dehiscent. Due to its indumentum, leave shape, and anther morphology, C. molaui is placed in Section Lehmannina Pennell within subgenus Calceolaria. This is the only species of this section in southern Ecuador.

Enhancing Gene Expression Signatures In Cancer Prediction Models: Understanding And Managing Classification Complexity, Vidya P. Kamath

USF Tampa Graduate Theses and Dissertations

Cancer can develop through a series of genetic events in combination with external influential factors that alter the progression of the disease. Gene expression studies are designed to provide an enhanced understanding of the progression of cancer and to develop clinically relevant biomarkers of disease, prognosis and response to treatment. One of the main aims of microarray gene expression analyses is to develop signatures that are highly predictive of specific biological states, such as the molecular stage of cancer. This dissertation analyzes the classification complexity inherent in gene expression studies, proposing both techniques for measuring complexity and algorithms for reducing …

The Impact Of Adolescent Nicotine Exposure On Drug Dependence In Adulthood, Mai Alajaji

Theses and Dissertations

Nicotine is one of the first and most commonly abused drugs in adolescence. According to The Center for Disease Control, every day more than 6000 adolescents try their first cigarette and over 3000 of them become daily smokers. Smoking among adolescents is a strong predictor of future drug abuse and dependence in adulthood. A number of studies has suggests that adolescents pre-exposed to nicotine may suffer permanent disruption of the brain’s reward systems through changes in dopamine receptor function. We hypothesize that nicotine exposure during adolescence causes long lasting neurobiological alterations that increase the likelihood of cocaine use in adulthood. …

Role Of Xrcc3 In Acquisition And Maintenance Of Invasiveness Through Extracellular Matrix In Breast Cancer Progression, Siddharth Saini

Theses and Dissertations

Acquisition of invasiveness through extracellular matrix is a crucial characteristic of transition to malignancy in the breast. It was previously shown that Polo-like kinase 1 (PLK-1), a mitotic kinase and genome stability regulator, is involved in acquisition of invasiveness in a breast cell model (HMT-3522 cell line) of pre-invasive to invasive transition. This and other data led to the suggestion that a new class of genes called GISEM for Genome Instability and Extracellular Matrix Invasiveness may exist. Previous lab data show that XRCC3 is found downregulated in progression from preinvasive to invasive phenotype. This led to the hypothesis that XRCC3 …

Data Reduction For Spectral Clustering To Analyze High Throughput Flow Cytometry Data, Habil Zare, Parisa Shooshtari, Arvind Gupta, Ryan R. Brinkman

Paediatrics Publications

Background: Recent biological discoveries have shown that clustering large datasets is essential for better understanding biology in many areas. Spectral clustering in particular has proven to be a powerful tool amenable for many applications. However, it cannot be directly applied to large datasets due to time and memory limitations. To address this issue, we have modified spectral clustering by adding an information preserving sampling procedure and applying a post-processing stage. We call this entire algorithm SamSPECTRAL.Results: We tested our algorithm on flow cytometry data as an example of large, multidimensional data containing potentially hundreds of thousands of data points (i.e., …

Multiple Mechanisms Of Consciousness: The Neural Correlates Of Emotional Awareness., Jayna M Amting, Steven G Greening, Derek G V Mitchell

Brain and Mind Institute Researchers' Publications

Emotional stimuli, including facial expressions, are thought to gain rapid and privileged access to processing resources in the brain. Despite this access, we are conscious of only a fraction of the myriad of emotion-related cues we face everyday. It remains unclear, therefore, what the relationship is between activity in neural regions associated with emotional representation and the phenomenological experience of emotional awareness. We used functional magnetic resonance imaging and binocular rivalry to delineate the neural correlates of awareness of conflicting emotional expressions in humans. Behaviorally, fearful faces were significantly more likely to be perceived than disgusted or neutral faces. Functionally, …

Report Of The Second Report On The Ninth Meeting Of The Adhoc Open-Ended Working Group On Access And Benefit-Sharing, Convention On Biological Diversity

Latin American Energy Dialogue, White Papers and Reports

This document is the meeting minutes from the Convention on Biological Diversity's draft of the protocol on access to genetic resources and the fair and equitable sharing of benefits from their utilization.

A Multi-Objective Evolutionary Algorithm To Solve Complex Optimization Problems, Koyel Chaudhuri

Electronic Theses and Dissertations Archive

Multi-objective optimization problem formulations reflect pragmatic modeling of several real-life complex optimization problems. In many of them the considered objectives are competitive with each other; emphasizing only one of them during solution generation and evolution incurs high probability of producing a one-sided solution, which is unacceptable with respect to other objectives. An appropriate solution to the multi-objective optimization problem is to investigate a set of solutions that satisfy all of the competing objectives to an acceptable extent, where no solution in the solution set is dominated by others in terms of objective optimization. In this work, we investigate well known …

Infant Vocal Development In A Dynamic Communication System: Vocal Exploration Activities In Various Social Settings, Kyounghwa Kwon

Electronic Theses and Dissertations Archive

Pre-linguistic vocal productions are the manifestations of a unique capacity ofhuman infants.The uniqueness is characterized by voluntaryvocal play and systematic repetition which serveno fixed functions.Such pre-linguistic infant vocalizations are the product of complex processes of internal vocal exploration activities and external social interactions. However,very few studies have incorporated both aspects to understand the nature of early vocal development. Therefore, the current study aims to investigate the relationship between vocal category development and exploration activities in varying social communication circumstances, using a developmentally appropriate coding scheme for infant vocalization.The vocalizations of 7 infants at three different ages (approximately 4, 7, and …

The Alestle - Vol. 62 No. 43 - 07/28/2010, Southern Illinois University Edwardsville

The Alestle

Vol. 62 No. 43

Thinking Outside The Curve, Part I: Modeling Birthweight Distribution, Richard Charnigo, Lorie W. Chesnut, Tony Lobianco, Russell S. Kirby

Statistics Faculty Publications

BACKGROUND: Greater epidemiologic understanding of the relationships among fetal-infant mortality and its prognostic factors, including birthweight, could have vast public health implications. A key step toward that understanding is a realistic and tractable framework for analyzing birthweight distributions and fetal-infant mortality. The present paper is the first of a two-part series that introduces such a framework.

METHODS: We propose describing a birthweight distribution via a normal mixture model in which the number of components is determined from the data using a model selection criterion rather than fixed a priori.

RESULTS: We address a number of methodological issues, including how the …

Stereoscopic Particle Image Velocimetry Analysis Of Healthy And Emphysemic Acinus Models, Emily Jean Berg

Theses

Inhaled particles reaching the alveolar walls have the potential to cross the blood gas barrier and enter the blood stream. Pulmonary dosimetry, however, is not well understood. Numerical and experimental studies shed some light on the mechanisms of particle transport, but realistic geometries have not been investigated. In order to accurately predict particle deposition, the characteristics that affect deposition need to be understood. This includes, but is not limited to, fluid flow, lung morphology, breathing conditions, and particle concentration. Various geometries have been used for research, but very few are close representations to in vivo geometry. Most studies have used …

Mathematical And Statistical Analysis Of Random Coils In Proteins, Giang-Huang Dang

Theses (Pre-2016)

The three dimensional structure of a protein plays an essential role in the function of that protein. The primary structure of a protein is the sequence of amino acid residues that make up the protein. Secondary structures are stable arrangements of amino acid residues. Alpha-helices, beta-bridges, beta-sheets, turns, bends are all well defined secondary structures. But there are segments of protein that do not fall into any of the above recognized secondary structures. These are called random coils. This term does not mean there is no structure or at least we cannot claim there is no inherent structure. All we …