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2010

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A General Approach For Receptor And Antibody-Targeted Detection Of Native Proteins Utilizing Split-Luciferase Reassembly, Cliff I. Stains, Jennifer L. Furman, Jason R. Porter, Srivats Rajagopal, Richard T. Wyatt, Indraneel Ghosh Jan 2010

A General Approach For Receptor And Antibody-Targeted Detection Of Native Proteins Utilizing Split-Luciferase Reassembly, Cliff I. Stains, Jennifer L. Furman, Jason R. Porter, Srivats Rajagopal, Richard T. Wyatt, Indraneel Ghosh

Cliff Stains Publications

The direct detection of native proteins in heterogeneous solutions remains a challenging problem. Standard methodologies rely on a separation step to circumvent nonspecific signal generation. We hypothesized that a simple and general method for the detection of native proteins in solution could be achieved through ternary complexation, where the conditional signal generation afforded by split-protein reporters could be married to the specificity afforded by either native receptors or specific antibodies. Toward this goal, we describe a solution phase split-luciferase assay for native protein detection, where we fused fragmented halves of firefly luciferase to separate receptor fragments or single-chain antibodies, allowing …

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(Trans)Forming Traditional Interpretations Of Title Vii: “Because Of Sex” And The Transgender Dilemma, Mary Kristen Kelly Jan 2010

(Trans)Forming Traditional Interpretations Of Title Vii: “Because Of Sex” And The Transgender Dilemma, Mary Kristen Kelly

Duke Journal of Gender Law & Policy

No abstract provided.

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The Role Of Motivation In The Learning Of English As A Second Language, Nadzrah Ahmad Bustami Jan 2010

The Role Of Motivation In The Learning Of English As A Second Language, Nadzrah Ahmad Bustami

Student Works (2010-2019)

Motivation is important in language learning. This present study aims to investigate learners’ motivation in the learning of English as a second language at university level and the factors that affect their motivation. The study particularly focuses on six motivation components: integrative motivation, instrumental motivation, effort, valence, expectancy and ability. The present study also aims to explore the differences in motivation level between female and male participants. 189 students participated in this quantitative study. The survey method used was the administration of the Motivation Scale (Wen, 1996 and modified by Shaaban and Ghaith, 2000). The results of this study revealed …

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Preservice Secondary School Mathematics Teachers' Subject Matter Knowledge Of Perimeter And Area., Wun Thiam Yew Jan 2010

Preservice Secondary School Mathematics Teachers' Subject Matter Knowledge Of Perimeter And Area., Wun Thiam Yew

Student Works (2010-2019)

The purpose of this study was to investigate preservice secondary school mathematics teachers (PSSMTs)' subject matter knowledge (SMK) of perimeter and area. Specifically, this study aimed to investigate PSSMTs’ five basic types of knowledge of perimeter and area, namely conceptual knowledge (CK), procedural knowledge (PK), linguistic knowledge (LK), strategic knowledge (SK), and ethical knowledge (EK). This study also aimed to investigate PSSMTs' levels (low, medium, high) of SMK of perimeter and area. Data of this study was collected using clinical interview technique. Interview sessions were recorded using digital video camera and tape recorder. Subjects of this study consisted of eight …

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(2-1,1-Bis(Hydroxy-Meth-Yl)-2-Oxidoeth-Ylimino-Meth-Yl-4-Chloro-Phenolato-Kap Pan,O,O')Dibutyl-Tin(Iv), R. Reisi, Misni Misran, K.M. Lo, S.W. Ng Jan 2010

(2-1,1-Bis(Hydroxy-Meth-Yl)-2-Oxidoeth-Ylimino-Meth-Yl-4-Chloro-Phenolato-Kap Pan,O,O')Dibutyl-Tin(Iv), R. Reisi, Misni Misran, K.M. Lo, S.W. Ng

Research Publications (2006 to 2010)

The Schiff base ligand in the title compound, Sn(C4H 9)2(C11H12ClNO4), chelates to the Sn atom through the two deprotonated O atoms, as well as through the N atom, to confer an overall cis-C2SnNO2 trigonal-bipyramidal geometry at tin C - Sn - C = 130.3 (1)°. The hydr-oxy groups engage in O - H�O hydrogen bonding with the O atoms of adjacent mol-ecules, generating a chain running along the c axis.

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(Αr,4r,4ar,6as,7r,8s,10r,11s)-Methyl Α-Acetoxy-4-(3-Furanyl)-10-Hydroxy-4a,7,9,9-Tetramethyl-2,13-Dioxo-1,4,4a,5,6,6a,7,8,9,10,11,12-Dodecahydro-7,11-Methano-2h-Cycloocta[F][2]Benzopyran-8-Acetate (6-O-Acetylswietenolide) From The Seeds Ofswietenia Macrophylla, B.H. Goh, H. Abdul Kadir, S.N. Abdul Malek, S.W. Ng Jan 2010

(Αr,4r,4ar,6as,7r,8s,10r,11s)-Methyl Α-Acetoxy-4-(3-Furanyl)-10-Hydroxy-4a,7,9,9-Tetramethyl-2,13-Dioxo-1,4,4a,5,6,6a,7,8,9,10,11,12-Dodecahydro-7,11-Methano-2h-Cycloocta[F][2]Benzopyran-8-Acetate (6-O-Acetylswietenolide) From The Seeds Ofswietenia Macrophylla, B.H. Goh, H. Abdul Kadir, S.N. Abdul Malek, S.W. Ng

Research Publications (2006 to 2010)

The molecule of O-acetylswietenolide, C29H36O9, isolated from the seeds of Swietenia macrophylla, features four six membered rings connected together in the shape of a bowl; one of the inner rings adopts a twisted chair conformation owing to the C C double bond. The furyl substitutent is connected to an outer ring, and it points away from the bowl cavity. The hydroxy group is connected to a carbonyl O atom of an adjacent molecule by an O—H� � �O hydrogen bond, generating a chain running along the b axis.

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(2e)-1-(6-Chloro-2-Methyl-4-Phenylquinolin-3-Yl)-3-Phenylprop-2-En-1-One, Kong Wai Tan, E.R.T. Tiekink, A.J. Viji, S. Sarveswari, V. Vijayakumar Jan 2010

(2e)-1-(6-Chloro-2-Methyl-4-Phenylquinolin-3-Yl)-3-Phenylprop-2-En-1-One, Kong Wai Tan, E.R.T. Tiekink, A.J. Viji, S. Sarveswari, V. Vijayakumar

Research Publications (2006 to 2010)

In the title compound, C(25)H(18)ClNO, the conformation about the C=C double bond is E. Significant twists are evident in the molecule, with the benzene ring forming a dihedral angle of 53.92 (11)degrees with the quinolinyl residue. Further, the chalcone residue is approximately perpendicular to the quinolinyl residue [C(q)-C(q)-C(c)-O(c) torsion angle = -104.5 (3)degrees, where q = quinolinyl and c = chalcone]. In the crystal, the presence of C-H center dot center dot center dot O and C-H center dot center dot center dot pi interactions leads to supramolecular layers lying parallel to ((1) over bar 02).

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2,4,6,8-Tetrakis(4-Ethylphenyl)-3,7-Diazabicyclo 3.3.1 Nonan-9-One, Kong Wai Tan, E.R.T. Tiekink, K. Rajesh, V. Vijayakumar, A.P. Safwan Jan 2010

2,4,6,8-Tetrakis(4-Ethylphenyl)-3,7-Diazabicyclo 3.3.1 Nonan-9-One, Kong Wai Tan, E.R.T. Tiekink, K. Rajesh, V. Vijayakumar, A.P. Safwan

Research Publications (2006 to 2010)

The bicyclo[3.3.1] nonane ring in the title compound, C(39)H(44)N(2)O, adopts a chair-boat conformation with the four benzene rings being directed away from the carbonyl group. The presence of C-H center dot center dot center dot O contacts leads to helical supra-molecular chains along the b axis.

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2-Methylbenzimidazolium Thiocyanate, S.A. Shaker, Hapipah Mohd Ali, H. Khaledi Jan 2010

2-Methylbenzimidazolium Thiocyanate, S.A. Shaker, Hapipah Mohd Ali, H. Khaledi

Research Publications (2006 to 2010)

In the crystal structure of the title compound, C8H9N2+center dot SCN-, the nearly planar 2-methylbenzimidazolium cation [r.m.s. deviation = 0.0123 (4) angstrom] is perpendicular to a mirror plane and the methyl H atoms are disordered about the mirror plane with equal occupancies. The thiocyanate anion also lies on a mirror plane. N-H center dot center dot center dot N hydrogen bonds link the components into an infinite chain along the b axis.

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2-Methylbenzimidazolium Thiocyanate-2-Methylbenzimidazole (1/1), S.A. Shaker, Hapipah Mohd Ali, H. Khaledi Jan 2010

2-Methylbenzimidazolium Thiocyanate-2-Methylbenzimidazole (1/1), S.A. Shaker, Hapipah Mohd Ali, H. Khaledi

Research Publications (2006 to 2010)

In the crystal structure of the title compound, C(8)H(9)N(2)(+)center dot-SCN(-)center dot C(8)H(8)N(2), the three components are linked by intermolecular N-H center dot center dot center dot N and N-H center dot center dot center dot S hydrogen bonds into infinite chains along the c axis.

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A Biological Model To Improve Pe Malware Detection: Review, S.M. Abdulalla, L.M. Kiah, O. Zakaria Jan 2010

A Biological Model To Improve Pe Malware Detection: Review, S.M. Abdulalla, L.M. Kiah, O. Zakaria

Research Publications (2006 to 2010)

Malwares control computer systems by infecting system files. They take advantage of system compatibilities to ensure their survival from one version to another. The structure of the windows portable executable (PE) files between available versions of the windows operating system (OS) makes these files an easy target for malwares. Fields and codes of clean PE files are modified and changed after infection. Checking both changes and modifications is necessary to detect malwares with a minimum false alarm rate. This paper reviews models that propose to detect PE malwares. It discusses PE structure and identifies the fields and locations that are …

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Aetiology And Outcome Of Neonatal Cholestasis In Malaysia, Way Seah Lee, P.F. Chai, Christopher Chiong Meng Boey, Lai Meng Looi Jan 2010

Aetiology And Outcome Of Neonatal Cholestasis In Malaysia, Way Seah Lee, P.F. Chai, Christopher Chiong Meng Boey, Lai Meng Looi

Research Publications (2006 to 2010)

Introduction: Little is known about the epidemiology, causes and outcomes of neonatal cholestasis in the Asian population beyond Japan and Taiwan. Methods: This was a prospective, observational study on patients with neonatal cholestasis who were referred to the University of Malaya Medical Centre, Malaysia, between November 1996 and May 2004. Results: Biliary atresia (BA) (29 percent) and idiopathic neonatal hepatitis (38 percent) were the two commonest causes of neonatal cholestasis (n is 146) that were referred. Out of the 39 patients (27 percent of the total) who died at the time of review, 35 succumbed to end-stage liver disease. Three …

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Age At Menarche Of University Students In Bangladesh: Secular Trends And Association With Adult Anthropometric Measures And Socio-Demographic Factors, M.D.G. Hossain, S. Islam, S.A.W. Aik, T.K. Zaman, P.E. Lestrel Jan 2010

Age At Menarche Of University Students In Bangladesh: Secular Trends And Association With Adult Anthropometric Measures And Socio-Demographic Factors, M.D.G. Hossain, S. Islam, S.A.W. Aik, T.K. Zaman, P.E. Lestrel

Research Publications (2006 to 2010)

Age at menarche has been shown to be an important indicator for diseases such as breast cancer and ischaemic heart disease. The aim of the present study was to document secular trends in age at menarche and their association with anthropometric measures and socio-demographic factors in university students in Bangladesh. Data were collected from 995 students from Rajshahi University using a stratified sampling technique between July 2004 and May 2005. Trends in age at menarche were examined by linear regression analysis. Multiple regression analysis was used to assess the association of age at menarche with adult anthropometric measures and various …

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Agrobacterium-Mediated Transformation Of The Aquarium Plant Cryptocoryne Willisii With Gus And Gfp Genes, N. Khalid, S.M. Wong, N. Sahidin Jan 2010

Agrobacterium-Mediated Transformation Of The Aquarium Plant Cryptocoryne Willisii With Gus And Gfp Genes, N. Khalid, S.M. Wong, N. Sahidin

Research Publications (2006 to 2010)

The effects of different infection times (2, 4, 6, 8, and 10 min) and co-cultivation periods (1, 2, 3, and 4 days) on transformation ofCryptocoryne willisii with Agrobacterium tumefaciens strain LBA4404 harbouring pCAMBIA1304 were assessed. Fluorometric assay revealed that the highest expression of β-glucuronidase (GUS) enzyme driven by the CaMV 35S promoter can be achieved by 6 min infection time and one day of co-cultivation (582.1 ± 84.3 pmol 4MU/mg/min). Expression of green fluorescent protein (mgfp5) gene fused to 5' GUS gene was also observed. Transformants appeared green fluorescent under ultraviolet light (UV) excitation. Plantlets of C. willisii showing GFP …

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Analysis And Experiment Of Centrifugal Force For Microfluidic Elisa Cd Platform, F. Ibrahim, P. Jahanshahi, N. Abd Rahman, M.K.B. Abdul Kahar, M. Madou, A.A. Nozari, N. Soin, S.Z. Md Dawal, K. Abi Samra Jan 2010

Analysis And Experiment Of Centrifugal Force For Microfluidic Elisa Cd Platform, F. Ibrahim, P. Jahanshahi, N. Abd Rahman, M.K.B. Abdul Kahar, M. Madou, A.A. Nozari, N. Soin, S.Z. Md Dawal, K. Abi Samra

Research Publications (2006 to 2010)

This paper presents an analysis and experiment of centrifugal force for microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) on a compact-disc (CD) platform. The ELISA CD was designed based on the centrifugal force as a driving force while capillary force acts as preventing barrier. The CD composed of 2 layers: the substrate layer is made of PMMA and the top layer is laminated with an Adhesive Sealing Films (ASF) or thermal seal. The PMMA substrate was fabricated by CNC micromachining. The ELISA CD consists of 7 reservoirs: waste, detection, serum, conjugate, washing solution, substrate and stopping solution. In the experiment, the reservoirs …

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Application Of Electromagnetic Waves As A Solution To Some Agricultural Problems, B.M. Ida Idayu, S. Maryam Ashayer, E. Amin, H. Aliakbarian, H. Ameri Mahabadi, M. Mahmoud Jan 2010

Application Of Electromagnetic Waves As A Solution To Some Agricultural Problems, B.M. Ida Idayu, S. Maryam Ashayer, E. Amin, H. Aliakbarian, H. Ameri Mahabadi, M. Mahmoud

Research Publications (2006 to 2010)

A review of some applications of electromagnetic waves in agriculture is presented. There are many applications in the literature which are categorized in two sections of post-harvest indoor and pre-harvest outdoor applications. Taking a look at the applications suggested, there are still works to be done to industrialize the method. However, the proposed method is promising in special cases when the cost and hazards of other methods are high.

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Asian-American Conference On Environmental Protection 1978 (Sapporo, Japan), N.A. Rashid Jan 2010

Asian-American Conference On Environmental Protection 1978 (Sapporo, Japan), N.A. Rashid

Research Publications (2006 to 2010)

No abstract provided.

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Assessing The Perceptions Of A Biostatistics And Epidemiology Module: Views Of Year 2 Medical Students From A Malaysian University. A Cross-Sectional Survey, Aqil M. Daher, Farzana Amin Jan 2010

Assessing The Perceptions Of A Biostatistics And Epidemiology Module: Views Of Year 2 Medical Students From A Malaysian University. A Cross-Sectional Survey, Aqil M. Daher, Farzana Amin

Research Publications (2006 to 2010)

Background In the era of evidence based medicine, biostatistics and epidemiology are considered as the main elements aiding the health professional to design a research study, understand the literature, and make decisions about patient care. The aim of the study is to explore students' perception about this subject because it plays an important role in determining educational outcome. Methods Data were collected from a self-administered questionnaire distributed among 164 Year 2 medical students. The 5-point Likert scale anchored by Strongly disagree = 1 and Strongly agree = 5 included 36 questions in four domains designed to assess the perception of …

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Assessing The Value Of Dna Barcodes And Other Priority Gene Regions For Molecular Phylogenetics Of Lepidoptera, J.J. Wilson Jan 2010

Assessing The Value Of Dna Barcodes And Other Priority Gene Regions For Molecular Phylogenetics Of Lepidoptera, J.J. Wilson

Research Publications (2006 to 2010)

Background: Despite apparently abundant amounts of observable variation and species diversity, the order Lepidoptera exhibits a morphological homogeneity that has provided only a limited number of taxonomic characters and led to widespread use of nucleotides for inferring relationships. This study aims to characterize and develop methods to quantify the value of priority gene regions designated for Lepidoptera molecular systematics. In particular, I assess how the DNA barcode segment of the mitochondrial COI gene performs across a broad temporal range given its number one position of priority, most sequenced status, and the conflicting opinions on its phylogenetic performance. Methodology/Principal Findings: Gene …

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Banana, Gingers And Papaya Cell Cultures For High Throughput Agriculture, Norzulaani Khalid, W.W. Chee, F. Bokhari, T.S. Kiat, F.D. Idris, M. Mohamad, H. Ibrahim, S. Annuar, R.Y. Othman, N.A. Rahman Jan 2010

Banana, Gingers And Papaya Cell Cultures For High Throughput Agriculture, Norzulaani Khalid, W.W. Chee, F. Bokhari, T.S. Kiat, F.D. Idris, M. Mohamad, H. Ibrahim, S. Annuar, R.Y. Othman, N.A. Rahman

Research Publications (2006 to 2010)

There is considerable interest in developing plant cell cultures that could facilitate rapid propagation of elite materials and for the production of phytochemicals. In Malaysia, there is an urgent need for sufficient supply to meet the voluminous demand for seedlings which are important plantation and cash crops. Considering the cost of labour and infrastructure in conventional plantation, propagation in liquid culture is a practical way to produce clonal propagules at a low cost. Plant cell cultures could also provide an alternative source of phytochemicals. These cultures will ensure continuous high quality compounds and will also prevent the extinction of the …

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Biodiesel Production From Waste Soybean Oil Biomass As Renewable Energy And Environmental Recycled Process, A.B.M. Sharif Hossain, Amru Nasrulhaq Boyce, A. Salleh, S. Chandran Jan 2010

Biodiesel Production From Waste Soybean Oil Biomass As Renewable Energy And Environmental Recycled Process, A.B.M. Sharif Hossain, Amru Nasrulhaq Boyce, A. Salleh, S. Chandran

Research Publications (2006 to 2010)

Alternative fuel is currently an important issue all over the world due to the efforts on reducing global warming which is contributed by the combustion of petroleum or petrol diesel. Biodiesel is non-toxic, biodegradable, produced from renewable sources and contributes a minimal amount of net green house gases, such as CO(2), SO(2) and NO emissions to the atmosphere. The study was carried out to produce biodiesel from waste/recycled oils to reduce the cost of biodiesel, waste and pollution. Some important variables such as volumetric ratio, types of reactants and catalytic activities were selected to obtain a high quality biodiesel fuel …

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Characterization Of Drug Resistant Salmonella Enterica Serotype Typhimurium By Antibiograms, Plasmids, Integrons, Resistance Genes And Pfge, Douadi Benacer, Kwai Lin Thong, Haruo Watanabe, Savithri Devi Puthucheary Jan 2010

Characterization Of Drug Resistant Salmonella Enterica Serotype Typhimurium By Antibiograms, Plasmids, Integrons, Resistance Genes And Pfge, Douadi Benacer, Kwai Lin Thong, Haruo Watanabe, Savithri Devi Puthucheary

Research Publications (2006 to 2010)

Forty-seven Salmonella Typhimurium (33 zoonotic, 14 clinical) strains were tested for antimicrobial resistance using the standard disk diffusion method. The presence of relevant resistance genes and class 1 integrons were investigated by using PCR. Pulsed-field gel electrophoresis (PFGE) and plasmid profiling were carried out to determine the genomic diversity of Salmonella Typhimurium. Approximately 57.4 of the S. Typhimurium strains were multidrug resistant (MDR) and showed high resistance rates to tetracycline (70.2), sulfonamides (57.4), streptomycin (53.1), ampicillin (29.7), nalidixic acid (27.6), kanamycin (23.4), chloramphenicol (21.2), and trimethoprim (19.1). Resistance towards cephalosporins was noted for cephalothin (27.6), cephradine (21.2), amoxicillin clavulanic acid …

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Characterizations Of Chitosan-Based Polymer Electrolyte Photovoltaic Cells, Abdul Kariem Arof, M.H. Buraidah, L.P. Teo, S.R. Majid, Rosiyah Yahya, R.M. Taha Jan 2010

Characterizations Of Chitosan-Based Polymer Electrolyte Photovoltaic Cells, Abdul Kariem Arof, M.H. Buraidah, L.P. Teo, S.R. Majid, Rosiyah Yahya, R.M. Taha

Research Publications (2006 to 2010)

The membranes 55 wt. chitosan-45 wt. NH 4 I, 33 wt. chitosan-27 wt. NH 4 I -40 wt. EC, and 27.5 wt. chitosan-22.5 wt. NH 4 I -50 wt. buthyl-methyl-imidazolium-iodide (BMII) exhibit conductivity of 3.73Ã� 10 -7, 7.34Ã� 10 -6, and 3.43Ã� 10 -5 S cm -1, respectively, at room temperature. These membranes have been used in the fabrication of solid-state solar cells with configuration ITO/ TiO 2 /polymer electrolyte membrane/ITO. It is observed that the short-circuit current density increases with conductivity of the electrolyte. The use of anthocyanin pigment obtained by solvent extraction from black rice and betalain from …

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Clinico-Pathological Evaluation Of Oral Melanotic Macule, Oral Pigmented Nevus And Oral Mucosal Melanoma, R.R. Buery, C.H. Siar, N. Katase, M. Fujii, H. Liu, M. Kubota, R. Tamamura, H. Tsujigiwa, H. Nagatsuka Jan 2010

Clinico-Pathological Evaluation Of Oral Melanotic Macule, Oral Pigmented Nevus And Oral Mucosal Melanoma, R.R. Buery, C.H. Siar, N. Katase, M. Fujii, H. Liu, M. Kubota, R. Tamamura, H. Tsujigiwa, H. Nagatsuka

Research Publications (2006 to 2010)

Focal pigmented melanocytic lesions rarely occur in the oral cavity but should not be taken for granted for they may represent markers or risks for oral mucosal melanoma (OMM). The study investigated the clinical and pathological features of focal pigmented lesions of melanocytic origin focusing on oral melanotic macule (oral MM), oral pigmented nevus (OPN) and OMM. Immunohistochemistry was employed with S100, HMB-45, Melan A, c-kit and Ki-67. Oral MM mostly occurred on the gingiva and buccal mucosa while OPN mostly occurred on the buccal mucosa. OMM occurred on the palate and gingiva. A female gender predilection was observed in …

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Coconut Waste As A Source For Biodiesel Production, S. Sulaiman, Abdul Aziz Abdul Raman, Mohamed Kheireddine Aroua Jan 2010

Coconut Waste As A Source For Biodiesel Production, S. Sulaiman, Abdul Aziz Abdul Raman, Mohamed Kheireddine Aroua

Research Publications (2006 to 2010)

Biodiesel industry needs a cheaper and economical viable raw material that can replace the currently used vegetable oil. Obtaining cheaper raw materials are one of the continuous targets of many biodiesel producing facilities since 70 to 95 of the production costs are attributed to raw materials. One of the main options is to use waste material from animal and plant sources. In this study, coconut waste is used to produce biodiesel using methanol and KOH. The oil content in coconut waste varies from 10-11 wt. The highest yield, 64 is achieved with 5 wt of KOH within 3 hr by …

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Crystallization And Initial X-Ray Diffraction Analysis Of A Mannose-Binding Lectin From Champedak, M. Gabrielsen, P.S. Abdul-Rahman, N.W. Isaacs, Onn Haji Hashim, R.J. Cogdell Jan 2010

Crystallization And Initial X-Ray Diffraction Analysis Of A Mannose-Binding Lectin From Champedak, M. Gabrielsen, P.S. Abdul-Rahman, N.W. Isaacs, Onn Haji Hashim, R.J. Cogdell

Research Publications (2006 to 2010)

Mannose-binding lectin from champedak (Artocarpus integer) is a homotetramer with a single-monomer molecular weight of 16 800 Da. Previous work has shown it to bind IgE and IgM, as well as being a mitogen of T cells in humans. Champedak mannose-binding lectin has successfully been used to detect altered glycosylation states of serum proteins. The protein was crystallized at 293 K in space group P2(1)2(1)2(1) (unit-cell parameters a = 76.89, b = 86.22, c = 95.37 angstrom) and the crystals diffracted to 2.0 angstrom resolution.

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Detection Of Free Living Amoebae, Acanthamoeba And Naegleria, In Swimming Pools, Malaysia, Init Ithoi, Y.L. Lau, A.A. Fadzlun, A.I. Foead, R.S. Neilson, Veeranoot Nissapatorn Jan 2010

Detection Of Free Living Amoebae, Acanthamoeba And Naegleria, In Swimming Pools, Malaysia, Init Ithoi, Y.L. Lau, A.A. Fadzlun, A.I. Foead, R.S. Neilson, Veeranoot Nissapatorn

Research Publications (2006 to 2010)

This study reports the detection of Acanthamoeba and Naegleria species in 14 swimming pools around Petaling Jaya and Kuala Lumpur, Malaysia. Sampling was carried out at 4 sites (the platforms (P), wall (W), 1 meter from the wall (1) and middle (2)) of each swimming pool. These free living amoebae (FLA) were detected under light and inverted microscopes after being cultured on the surface of non-nutrient agar lawned with Escherichia coli. Acanthamoeba species were detected in higher number of culture plates from all sampling sites of all the swimming pools. While Naegleria, were detected in fewer culture plates at 3 …

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Development Of Lightweight Concrete Using Industrial Waste Material, Palm Kernel Shell As Lightweight Aggregate And Its Properties, Ubagaram Johnson Alengaram, Hilmi Mahmud, Mohd Zamin Jumaat Jan 2010

Development Of Lightweight Concrete Using Industrial Waste Material, Palm Kernel Shell As Lightweight Aggregate And Its Properties, Ubagaram Johnson Alengaram, Hilmi Mahmud, Mohd Zamin Jumaat

Research Publications (2006 to 2010)

Abstract-Agricultural industrial wastes produced after extracting palm oil from palm fruits known as palm kernel shell (PKS) are available in large quantities in Indonesia, Malaysia, Nigeria and other tropical countries. Malaysia is the second largest palm oil producing country in the world. This paper reports the results of an investigation conducted to utilize the PKS as lightweight aggregate to produce grade30 concrete with density of about 1850kg/m3. The properties of both PKS and crushed granite aggregates were compared. The concrete produced using PKS referred to here after as palm kernel shell concrete (PKSC) and its properties were compared with properties …

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Differentiation Of Salmonella Enterica Based On Pcr Detection Of Selected Somatic And Flagellar Antigens, M.E.E. Nori, Kwai Lin Thong Jan 2010

Differentiation Of Salmonella Enterica Based On Pcr Detection Of Selected Somatic And Flagellar Antigens, M.E.E. Nori, Kwai Lin Thong

Research Publications (2006 to 2010)

Serotyping is the basis of Salmonella surveillance. However, the limitations of the traditional serotyping have stimulated rapid research and development in DNA-based serotyping. The aim of this study was to apply a combination of sequential multiplex PCRs targeting the O, H and Vi antigens to serotype a panel of 122 recently isolated human- and foodborne-Salmonella strains. The mPCR that targets the o (genes wzxC2, rfbJ, prt, tyv, wzxE, wzxC1, prt) and Vi (viaB) antigens successfully subtyped the strains into serogroups C2 (n = 35, 28.7), B (n = 33, 27.1), D9 (n = 29, 22.9), E (n = 21, 17.2), …

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Dna Fingerprinting Of Human Isolates Of Burkholderia Pseudomallei From Different Geographical Regions Of Malaysia, K.H. Chua, K.H. See, Kwai Lin Thong, S.D. Puthucheary Jan 2010

Dna Fingerprinting Of Human Isolates Of Burkholderia Pseudomallei From Different Geographical Regions Of Malaysia, K.H. Chua, K.H. See, Kwai Lin Thong, S.D. Puthucheary

Research Publications (2006 to 2010)

Melioidosis is an infectious disease caused by Burkholderia pseudomallei and endemic in Southeast Asia. One hundred and forty six clinical isolates of B. pseudomallei from different states in Malaysia were obtained and molecular typing was carried out using pulsed-field gel electrophoresis (PFGE). Overall, nine clusters were successfully identified. Burkholderia pseudomallei isolates used in this study were found to be genetically diverse and there were differences in the clusters of isolates from peninsular and east Malaysia. BS9 cluster was the most common cluster and found in all the states while BS2 cluster only existed in a particular state. Based on the …

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