Digital Commons Network^™

Phylloplanins: Novel Antifungal Proteins On Plant Leaf Surfaces, Ryan William Shepherd

University of Kentucky Doctoral Dissertations

Secreted surface proteins are an innate immune defense component employed by animals to inhibit invading microbes. Surface proteins have not been documented in plants, even though the aerial leaf surface, or phylloplane, is a major site of pathogen ingress. We have discovered novel proteins, termed phylloplanins, which accumulate on leaf surfaces of Nicotiana tabacum, and we have isolated the gene Phylloplanin that is unique in gene databases. Natural and E. coli-expressed phylloplanins inhibit spore germination and limit leaf infection by the oomycete pathogen Peronospora tabacina.

We investigated the site of phylloplanin biosynthesis using biochemical techniques. These techniques …

Long Wave Animation, Anwar Shaikh Phd

Archives of Anwar Shaikh

This collection includes data on US and UK golden waves, 1786–2010.

Queer Appalachia: Toward Geographies Of Possibility, Mathias J. Detamore

University of Kentucky Doctoral Dissertations

Stereotypes about Appalachia abound through dubious and reductive representations of the ‘hillbilly’ icon. Sexuality and how it functions in Appalachia is usually cast from the outside as wild, violent, bestial, incestuous and generally base. Movies such as Deliverance and television shows such as The Beverly Hillbillies and The Dukes of Hazard render images of Appalachian sexuality as hyper-sexual, both naive and violent. These images of Appalachian sexual ignorance and violence that permeate popular culture have had problematic and reductive implications for rural gay/trans Appalachian folk. Mainstream gay culture has often used the perceived meanings of these images to circumscribe and …

The Advent Of Gene Patenting: Putting The Great Debate In Perspective, Brian Zadorozny

SMU Science and Technology Law Review

No abstract provided.

Purchasing Subscription Reference Databases In The Iowa Elementary Public School Library, Michaela B. Seeman

Honors Program Theses

With so many reference materials available for the elementary public school library, making subscription reference database purchasing decisions can be an overwhelming task. The purpose of this research study was to examine how the 15 participating Iowa teacher librarians made subscription reference database purchasing decisions and the use they observe of various reference materials by students and staff influencing these decisions. To answer these questions, I conducted a survey asking Iowa public elementary school teacher librarians how they decided what to purchase and criteria they used for examining reference materials such as subscription reference databases. The population used for this …

Antidepressant Stimulation Of Cdp-Diacylglycerol Synthesis Does Not Require Monoamine Reuptake Inhibition, Ashiwel S. Undieh, Marwa A. Aboukhatwa

Publications and Research

Background: Recent studies demonstrate that diverse antidepressant agents increase the cellular production of the nucleolipid CDP-diacylglycerol and its synthetic derivative, phosphatidylinositol, in depression-relevant brain regions. Pharmacological blockade of downstream phosphatidylinositide signaling disrupted the behavioral antidepressant effects in rats. However, the nucleolipid responses were resistant to inhibition by serotonin receptor antagonists, even though antidepressant-facilitated inositol phosphate accumulation was blocked. Could the neurochemical effects be additional to the known effects of the drugs on monoamine transmitter transporters? To examine this question, we tested selected agents in serotonin-depleted brain tissues, in PC12 cells devoid of serotonin transporters, and on the enzymatic activity of …

Virtual Contacts In Patent Cases: How Should Internet-Related Contacts Affect The Personal Jurisdiction Analysis?, Megan M. La Belle

Scholarly Articles

In the 1990s, when the Internet was still considered novel, courts struggled with the question of how Internet-related contacts should be treated in the personal jurisdiction analysis. So when Zippo Manufacturing v. Zippo DOT Com established an apparently easy-to-apply test for deciding whether a defendant’s virtual contacts are sufficient for personal jurisdiction, many courts embraced it . To date, however, the Federal Circuit has neither adopted nor rejected the Zippo approach, leaving litigants and lower courts in patent cases with little guidance on the issue. Although a recent decision suggests that the Federal Circuit recognizes the limitations of Zippo, it …

Salinity Tolerance And Osmotic Response Of The Estuarine Hermit Crab Pagurus Maclaughlinae In The Indian River Lagoon, Florida, Sarah E. Rhodes-Ondi, Richard L. Turner

Ocean Engineering and Marine Sciences Faculty Publications

Pagurus maclaughlinae is the most common hermit in the Indian River Lagoon System. Wide variations in lagoonal salinity make it likely that P. maclaughlinae is euryhaline and that other hermit species in the area are more stenohaline, at least in some stages of their life histories. In a study of salinity tolerance, crabs were held unfed at salinities of 5–50 (25 control) for up to 30 d. Based on survivorship curves, P. maclaughlinae tolerated acute exposure to salinities of 10–45 for up to 18 d, and survivorship up to 30 d at 20–45 equaled or exceeded survivorship of the control. …

The Loss Of Egypt’S Children, Cindy Ragab

Human Rights & Human Welfare

Under the fierce rays of the desert sun, in the heat of the summer, young children are forced to remove pests from cotton crops for eleven hours per day, search for recyclable goods among animals and the pungent stench of city dumps, and are sold to elderly male tourists through temporary marriages by their parents. This is the hideous reality for millions of child laborers in Egypt. Child labor is a manifestation of the pains of extreme poverty on the world’s most vulnerable population. Childhood is lost. Children are forced to take on responsibilities that in normal circumstances push adults …

The Distribution Of Gnrh And Erbeta In Pre- And Post-Pubertal Male Rats, Maysaa Nageeb

Master's Theses

The hypothalamic-pituitary- gonadal axis is intrinsic in regulating reproductive function. Each part orchestrates the synthesis and release of a key hormone. At the level of the hypothalamus, Gonadotropin- releasing hormone (GnRH) is released and it is the

most upstream regulator of sexual maturation. GnRH neurons express a type of estrogen receptor, ERbeta, which suggests that estradiol (E2) plays a direct role in modulating GnRH function (Herbison and Pape 2001). The objective of this study was to determine if gonadal steroid hormones regulate the expression of ERbeta and GnRH mRNA in the male brain during pubertal development. In-situ hybridization was used …

Dna Replication And Genome Maintenance Of Human Papillomavirus Type 16 In Mammalian Cells, Daraporn Pittayakhajonwut

School of Biological Sciences: Dissertations, Theses, and Student Research

Human papillomavirus (HPV) is a major causative agent of cervical cencer and a number of human cancers. The high‐risk types can be detected in more than 90% of cervical cancer, of which HPV16 is the most common found. The virus establishes a latent infection in basal epithelial cells in which the viral genomes can be stably maintained as extrachromosomal DNA for decades before the development of cancer. Several attempts have been made in bovine papillomavirus (BPV) to understand how the virus can maintain its genome at constant copy numbers in dividing cells. The viral protein E2 has been proposed to …

Phospholipase D Signaling In T Cells, Uma Chandrasekaran

Dissertations

Antigen stimulation of T lymphocytes induces the activation of phospholipase D (PLD) signaling. Phospholipase D (PLD) is a phosphodiesterase that catalyzes the conversion of phosphatidyl choline (PC) to phosphatidic acid (PA). PA is an important lipid second messenger and is known to mediate a variety of cellular functions. However, the specific role of PA in T lymphocytes has not been established. Previous studies indicated differential requirement for TCR induced PLD signaling in regulatory and non-regulatory T cells. Inhibition of TCR induced PLD signal preferentially suppressed the growth of non-regulatory T cells while allowing the proliferation of regulatory T cells in …

B Lymphocyte Development In Galt, Venkata Arunachalam Yeramilli

Dissertations

In rabbits, the primary antibody repertoire is generated in the gut-associated lymphoid tissues (GALT), where bone marrow (BM)-derived B cells undergo a proliferative expansion and somatically diversify the immunoglobulin genes. Unlike in other species, B lymphopoiesis in rabbit arrests a few months after birth, and it is unclear how the peripheral B cell compartment is maintained when there is no influx of newly-made B cells from the BM.

For my dissertation, I investigated how B cells develop in the GALT of rabbits, and how they are maintained in adults after the arrest of lymphopoiesis. To identify cellular signals that promote …

Ethanol Impairs Mechanisms Of Macrophage Phagocytosis And Cytokine Production, John Karavitis

Dissertations

Extensive evidence indicates that alcohol (ethanol) consumption affects human health by altering normal physiological functions of the immune system. This study investigated the effect of a single in vivo exposure of macrophages to clinically relevant levels of ethanol (1.2 and 2.2 g/kg). Following 3 hour exposure, both doses of ethanol decreased ex vivo TNFα production by splenic and alveolar macrophages (AMs). Interestingly, the higher dose of ethanol resulted in sustained suppression of LPS-induced TNFα production at 3 and 6 hours post ethanol administration, as well as decreased IL-6 and IL-12 production after 6 hours, compared control treated groups. LPS or …

Uncommon Sense In Renaissance English Literature, Eric Byville

Dissertations

My project explores the distinctive union of Senecan tragedy and Elizabethan satire in Renaissance English drama, particularly the works of John Marston and William Shakespeare. Unlike Ben Jonson, who incorporated both Senecan tragedy and Elizabethan satire in his drama but did so in different plays (Catiline, Every Man Out), Marston and Shakespeare combined the two traditions in one and the same play, such as the former's Antonio's Revenge (1600) and The Malcontent (c. 1603) and the latter's Troilus and Cressida (1601) and Timon of Athens (c. 1606). They recognized and exploited a deep compatibility between the two traditions, a compatibility …

Your Change Is Still Behind: Futurity In Early Modern Literature, Tripthi Pillai

Dissertations

A study of Renaissance literature's engagement with temporality, my project is a critical evaluation of the concept of early modern futurity, of which I propose three categories: "Material futurity"; "Biological futurity"; and "Political futurity." In the moments that I identify in texts composed during the Tudor and early Stuart reigns in England, I demonstrate that the future--as an idea--structures individuals' actions and ruptures social formations. Futurity, which I define as a play of multiple desires that exist simultaneously within our present beings, is a volatile agent of imagination in early modern literature. Futurity collides with the cultural sites of memory …

Characterization Of Tram, A Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts With The B. Fragilis Putative Coupling Protein Bcta And The B. Fragilis Relaxase Protein Bmph, Mai Thi Thanh Nguyen

Dissertations

Bacteroides spp. organisms, the most prominent bacteria of the human colon, are reservoirs of antibiotic resistance genes which promiscuously transfer within and from the genus via conjugation. The B. fragilis clinical isolate LV23 harbors a 37kb conjugative transposon, BTF-37. Two important conjugal apparatus proteins encoded by BTF-37 are BctA, a predicted coupling protein (CP) that powers DNA translocation, and ORF7 (TraM). In this study, we demonstrated that TraM exhibits characteristics of a required conjugal apparatus protein including 1) upregulated expression under conjugation conditions; 2) localization to the bacterial inner membrane; 3) interaction with the putative coupling protein BctA and the …

Morphogenesis Of The Bacillus Anthracis Spore, Michael Mallozzi

Dissertations

With the ability to shut down metabolism, survive exposure to high degrees of heat, ultra-violet and ionizing radiation, digestive enzymes, and antimicrobials, the bacterial spore is one of the most-resistant forms of life on earth. Although most spores are harmless, several species' spores cause serious diseases such as tetanus, anthrax, gas gangrene, botulism, and diarrhea. Spore-forming bacteria differentiate into spores in response to stresses (especially starvation) in a multi-stage developmental pathway involving the coordinated expression of hundreds of genes and the formation of specialized protective organelles which surround and protect the spore during dormancy. The Bacillus anthracis spore (the causative …

Involvement Of The Nmda Receptor In Moderate Ethanol Preconditioning-Dependent Neuroprotection From Amyloid-Beta In Vitro, Robert Matthew Mitchell

Dissertations

Alzheimer's disease (AD) is a progressive, mentally crippling, and eventually fatal form of dementia with growing prevalence in aging populations. In 2009 it was estimated that 5.3 million Americans have AD, with 5.1 million older than age 65 and 200,000 under 65. That accounts for 1 in 8 adults over 65. Furthermore, AD costs Americans 148 billion dollars in direct expenses, and is the leading cause of dementia and the 6th leading cause of death. Since improved medical care in general is increasing average life span and age is the primary risk factor for AD, there is need for basic …

Hindbrain Serotonin Neurons Activate 5-Ht1a Receptors In The Nucleus Tractus Solitarius (Nts) To Modulate Sympathetic And Ventilatory Recovery Following Hypotensive Hemorrhage, Ling-Hsuan Kung

Dissertations

Trauma is the leading cause of death of young people in the United States. Most such deaths result either from the initial blood loss or from secondary reperfusion injury following resuscitation.

Currently, the standard treatments for hypovolemic shock include volume resuscitation and using of vasoconstrictors to raise pressure. However, these treatments can exacerbate reperfusion injury, and require technical care when apply to patients. Therefore, novel therapies that can safely restore perfusion pressure are needed.

Our lab has found that 5-HT1A-receptor agonist, 8-OH-DPAT, increases perfusion pressure and improves acid-base balance in hypovolemic rats by stimulation of sympathetic-mediated increases in venous tone. …

Interpretive Teleology Without Eschatology: A Study Of The Hermeneutic Strategies Of Nietzsche's Genealogical Project, Darin Sean Mcginnis

Dissertations

In contemporary philosophy, there exists a vigorous debate between those thinkers who advocate the need for a truth that exists outside of historical forces and those who hold that all understanding must be historical in its basis. My research is focused on deciphering this problem by providing a description of how understanding and interpretation occur. More specifically, I have proposed a project that explores how the philosophical writings of Friedrich Nietzsche can give some insight into this problem of understanding truth. I argue that Nietzsche develops a method of interpretation - a hermeneutics - that focuses on the manner in …

Roman Law And The Legal World Of The Romans, James G. Keenan

Classical Studies: Faculty Publications and Other Works

A review of Andrew. M Riggsby's book, Roman Law and the Legal World of the Romans.

Deconstructing Ihf-Mediated Inhibition Of The Complex Acs Promoter, David S. Thach

Dissertations

acs encodes a high affinity enzyme that permits survival during carbon starvation. As befits a survival gene, its transcription is subject to complex regulation. Previously, the Wolfe lab reported that CRP activates acs transcription by binding tandem DNA sites located upstream of the major acsP2 promoter and that the nucleoid protein IHF binds three specific sites located just upstream. The most proximal site (IHF III) exhibits reduced transcription compared to the full-length promoter or to a construct lacking all three IHF sites. The goal of my research was to understand how IHF III inhibits CRP-dependent acs transcription. First, I helped …

Developmental Characterization Of Cara Mitad: A Drosophila Nuclear Receptor Co-Regulator, Chhavi Chauhan

Dissertations

In insects and vertebrates, hormone titers drive cellular proliferation and differentiation events that guide proper development. Nuclear receptors (NR) respond to these hormone signals by activating cascades of gene expression, along with coregulator protein complexes. In Drosophila melanogaster, fluctuating titers of the steroid hormone ecdysone are responsible for coordinating the timing of organismal development. Despite major advances in our understanding of Drosophila NR activities, we lack essential knowledge of the coregulators that are required for their proper function.

We have recently identified the Drosophila cara mitad (cmi) (`dear half') gene. The deduced CMI protein is closely related to the N-terminal …

Mechanisms Of Ipsapirone-Mediated Neuroprotection Of Ethanol-Treated Fetal Rhombencephalic Neurons: Role Of Endogenous Antioxidants, Jongho Lee

Dissertations

Prenatal exposure to ethanol can cause serious damage to the developing central nervous system. Both in vivo and in vitro studies demonstrate the vulnerability of the developing serotonergic system to the damaging effects of ethanol. However, treatment with a serotonin-1A (5-HT1A) agonist prevents the ethanol-associated apoptosis in developing 5-HT neurons. One mechanism by which 5-HT1A agonists exert their neuroprotective effects appears to involve activation of the phosphatidylinositol 3'kinase (PI-3K) pro-survival pathway and activation of NF-κB dependent anti-apoptotic genes. Additional NF-κB dependent genes might also be involved with the neuroprotective effects of ipsapirone.

There is also considerable evidence that ethanol augments …

Business Ethics And Global Age Cosmopolitanism, Isaias Ramon Rivera

Dissertations

This dissertation is a study of business ethics and business education. It is particularly focused on the Business Administration degree and the business ethics literature, while also considering university students, professors, and business practitioners. The key problem I address is how to create and teach an ethics of Global Age Cosmopolitanism (GAC). I approach this problem through a philosophical analysis focusing on political, economic, and educational principles. It is my hope that such an ethics will cause university professors and students to become engaged citizens who seek to solve the many problems that affect society and to sustain structural justice …

Lysine Specific Demethylase-1 And The Brahma Chromatin Remodeling Complex Regulate Conserved Signaling Pathways During Drosophila Wing Development, Brenda Jean Curtis

Dissertations

The conserved SWI/SNF chromatin remodeling complex uses the energy from ATP hydrolysis to alter local chromatin environments by disrupting DNA-histone contacts. The Drosophila SWI/SNF counterpart, the Brahma complex, has been shown to have an essential role in regulating the proper expression of many developmentally important genes, including those required for eye and wing tissue morphogenesis. A temperature sensitive mutation in one of the core subunits, SNR1 (SNF5/Ini1/SMARC B1), results in reproducible wing patterning phenotypes that can be enhanced and suppressed by extragenic mutations. SNR1 functions as a regulatory subunit to modulate chromatin remodeling activities of the Brahma complex on target …

Quality Of Life And Health Outcomes In Overweight And Non-Overweight Children With Asthma., Amy Becker Manion

Dissertations

Over the last two decades the number of children and adolescents who are overweight has more than doubled. Currently, an estimated 18 percent of children and adolescents ages 6-19 years are overweight. Following this trend in childhood overweight, there has been an alarming increase in the number of children with asthma who are overweight. The increasing trend in both asthma and overweight has led to the suggestion of a causal relationship between the two. Childhood overweight has been found to have a profound negative impact on quality of life (QOL), yet there is a dearth of research regarding the impact …

Courting And Consorting With The Global: The Local Politics Of An Emerging World Heritage Site In Sulawesi, Indonesia, Kathleen M. Adams

Anthropology: Faculty Publications and Other Works

I begin this chapter with a vignette concerning the events that led to the selection of a particular Toraja hamlet (known as Ke'te' Kesu') for tentative inclusion on UNESCO's List of World Heritage Sites. In this portion of the chapter I also unpack some of the local reactions to this selection and contrast these reactions with an analysis of UNESCO conception~ and assumptions pertaining to World Heritage Sites, many of which are entwined with romantic assumptions about ancient life-ways under siege by the contemporary world. I then turn to trace the historyofKe'te' Kesu', from its colonial roots to the present, …

Projected Loss Of A Salamander Diversity Hotspot As A Consequence Of Projected Global Climate Change, Joseph Milanovich, William E. Peterman, Nathan P. Nibbelink, John C. Maerz

Biology: Faculty Publications and Other Works

Significant shifts in climate are considered a threat to plants and animals with significant physiological limitations and limited dispersal abilities. The southern Appalachian Mountains are a global hotspot for plethodontid salamander diversity. Plethodontids are lungless ectotherms, so their ecology is strongly governed by temperature and precipitation. Many plethodontid species in southern Appalachia exist in high elevation habitats that may be at or near their thermal maxima, and may also have limited dispersal abilities across warmer valley bottoms.