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Spectroscopic Study Of The Dimerisation Process Of Iron Protoporphyrin Ix, K. Dziedzic-Kocurek, Hugh Byrne, A. Swiderski, J. Stanek

Articles

The commercial protoporphyrin IX, iron-ferriprotoporphyrin IX-chloride and synthesized iron porphyrin μ-oxo-dimers were examined by UV/vis absorption and fluorescence, Fourier transformed infrared spectroscopy, resonance Raman, X-ray absorption, Mössbauer spectroscopy and SQUID. The evidence of Fe-O-Fe antiferromagnetic coupling concluded from SQUID and Mössbauer in the case of samples containing dimerized forms confirmed the presence of the oxo-bridges. In this paper the results of UV/vis, fluorescence, Fourier transform infrared FTIR and Raman spectroscopies are reported and discussed. The study is based on the comparison of the free-base protoporphyrin IX, Fe-PPIX-Cl and the synthesized dimerized specimen. The vibrational modes in two energy regions i.e. …

The Bundle Dispersion Of Swnt On Interaction With P-Terphenyl, Theresa Hedderman, Anika Mostaert, Anne Shanahan, Hugh Byrne

Articles

This paper investigates the interaction and bundle dispersion of single walled carbon nanotubes (SWNT) produced by arc discharge and by the high pressure decomposition of carbon monoxide, often referred to as the HiPco method, in the presence of the molecule p-terphenyl. The study will show that the extent of SWNT bundle dispersion and the degree of interaction with p-terphenyl is related to the level of purity of the SWNT sample. This study compares the bundle dispersion and interaction of SWNT with p-terphenyl in their as produced state and after purification. A number of spectroscopic and microscopic techniques are used to …

Titanium Dioxide Nanoparticles Induce Oxidative Stress And Dna-Adduct Formation But Not Dna-Breakage In Human Lung Cells, Kunal Bhattacharya, Maria Davoren, Jens Boertz, Roel P.F. Schins, Eik Hoffmann, Elke Dopp

Articles

Titanium dioxide (TiO2), also known as titanium (IV) oxide or anatase, is the naturally occurring oxide of titanium. It is also one of the most commercially used form. To date, no parameter has been set for the average ambient air concentration of TiO2 nanoparticles (NP) by any regulatory agency. Previously conducted studies had established these nanoparticles to be mainly non-cyto- and -genotoxic, although they had been found to generate free radicals both acellularly (specially through photocatalytic activity) and intracellularly. The present study determines the role of TiO2-NP (anatase, ∅ < 100 nm) using several parameters such as cyto- and genotoxicity, DNA-adduct formation and generation of free radicals following its uptake by human lung cells in vitro. For comparison, iron containing nanoparticles (hematite, Fe2O3, ∅ < 100 nm) were used. The results of this study showed that both types of NP were located in the cytosol near the nucleus. No particles were found inside the nucleus, in mitochondria or ribosomes. Human lung fibroblasts (IMR-90) were more sensitive regarding cyto- and genotoxic effects caused by the NP than human bronchial epithelial cells (BEAS-2B). In contrast to hematite NP, TiO2-NP did not induce DNA-breakage measured by the Comet-assay in both cell types. Generation of reactive oxygen species (ROS) was measured acellularly (without any photocatalytic activity) as well as intracellularly for both types of particles, however, the iron-containing NP needed special reducing conditions before pronounced radical generation. A high level of DNA adduct formation (8-OHdG) was observed in IMR-90 cells exposed to TiO2-NP, but not in cells exposed to hematite NP. Our study demonstrates different modes of action for TiO2- and Fe2O3-NP. Whereas TiO2-NP were able to generate elevated amounts of free radicals, which induced indirect genotoxicity mainly by DNA-adduct formation, Fe2O3-NP were clastogenic (induction of DNA-breakage) and required reducing conditions for radical formation.

Effect Of Suburban Transit Oriented Developments On Residential Property Values, Shishir Mathur, Christopher Ferrell

Faculty Publications, Urban and Regional Planning

No abstract provided.

Providing Academic Leadership Through The Role Of Senior Lecturer 1 (Teaching) Using The Theme Of 'Sustainable Energy' As An Example, Jim Mcgovern

Articles

An academic leader should lead in their particular area and should love what they are doing. An example of leadership in the area of 'sustainable energy use' is presented that involves leading through identifying and participating in the development of emerging technologies. Excellent academic leadership can be provided by a Senior Lecturer through active research, incorporating the latest developments in student-centred learning in their work and providing example and encouragement for colleagues and learners.

The Effect Of Air Preheating In A Biomass Cfb Gasifier Using Aspen Plus Simulation, Wayne Doherty, Anthony Reynolds, David Kennedy

Articles

In the context of climate change, increasing efficiency and energy security, biomass gasification is likely to play an important role in energy production. Atmospheric circulating fluidised bed (CFB) technology was selected for the current study. The primary objective of this research is to develop a computer simulation model of a CFB biomass gasifier that can accurately predict gasifier performance under various operating conditions. An original model was developed using ASPEN Plus (Advanced System for Process ENgineering Plus). The model is based on Gibbs free energy minimisation. The restricted equilibrium method was used to calibrate the model against experimental data. This …

Biochemical Changes Of Antioxidant Enzymes In Common Carp (Cyprinus Carpio L.) After Heavy Metal Exposure, Rajamanickam Vinodhini, Muthuswamy Narayanan

Turkish Journal of Veterinary & Animal Sciences

Accidental industrial spills may lead to a high concentration of toxic metals in the aquatic environment, as well as affecting freshwater ecosystems with acute and chronic toxicity. Of all aquatic species, fish are particularly sensitive to waterborne contamination and are recognized as bioindicators for water quality monitoring. The objective of the present study was to evaluate the biochemical changes of the enzymatic defense systems in fresh water fish, the common carp (Cyprinus carpio L.), when they are exposed to a heavy metal contaminated aquatic system. The fish were systematically exposed to heavy metal solutions such as cadmium, lead, nickel, and …

Serum Zinc Concentrations In Dogs With Microsporum Canis Dermatophytosis: A Pilot Study, Kerem Ural, Mehmet Çağri Karakurum, Özkan Duru, Cenker Çağri Cingi, Ali̇ Evren Haydardedeoğlu

Turkish Journal of Veterinary & Animal Sciences

Zinc deficiency may result in alopecia, erythema, crusting, scaling, parakeratosis, inflammation of the skin, and impaired wound healing. Zinc deficiency also may impair the immune response against microorganisms and lead to fungal infections such as trichophytosis. The present study included male and female dogs (n = 7) of various breeds, ages, and diagnosed with Microsporum canis dermatophytosis and 4 healthy dogs used as the control group. The influence of dermatophytosis on serum zinc concentrations in dogs was investigated. Wood's lamp examination, fungal cultures, and additionally biopsy specimen were used to confirm the diagnosis and rule out other causes of the …

Postnatal Expression Pattern Of Ppar\Gamma Gene In Pigs, Junfang Jiang, Yongqing Jiang, Zhigang Zhu, Zirong Xu

Turkish Journal of Veterinary & Animal Sciences

PPAR\gamma is a member of the peroxisome proliferator-activated receptor subfamily of nuclear hormone receptors. PPAR\gamma has been showed to be a key regulator of adipocyte differentiation and lipid metabolism. The pattern of PPAR\gamma gene expression in different growth stages and its relation with adipose deposition in pigs is studied. Fifteen female Duroc×Landrace×Yorkshire pigs in 5 groups with 3 pigs in each grup, at live weight of 1, 30, 50, 70, and 90 kg were used to study the developmental gene expression of PPAR\gamma in subcutaneous adipose tissue by means of semi-quantitative RT-PCR. The results showed the PPAR\gamma mRNA levels of …

Effects Of Feeding Frequency On Nutrient Digestibility And Growth Performance Of Rainbow Trout (Oncorhynchus Mykiss) Fed A High Lipid Diet, Serap Ustaoğlu Tiril, Fi̇kret Alagi̇l

Turkish Journal of Veterinary & Animal Sciences

The effect of feeding frequency on nutrient digestibility and growth performance was investigated in rainbow trout (Oncorhynchus mykiss) fed a high lipid diet. The experimental diet contained 25.2% crude lipid and 40.7% crude protein. Two feeding frequencies were tested: 2 meals per day (Group I) and 6 meals per day (Group II). Fish were fed a restricted daily ration (1.5% of body weight) divided into equal meals. t the end of the experiment, the weight gain in Group I and II was 84.6% and 90.1%, respectively. There was no statistically significant difference between groups. The crude lipid and protein digestibility …

Effects Of Oocyte Source On The Developmental Competence Of In Vitro Matured Goat Oocytes Fertilized By The Intracytoplasmic Sperm Injection Technique, Abu Nasar Md. Aminoor Rahman, Ramli Bin Abdullah, Wan Embong Wan-Khadijah

Turkish Journal of Veterinary & Animal Sciences

This study evaluated the effects of oocyte source on in vitro maturation (IVM) and embryo developmental competence in goats by the intracytoplasmic sperm injection (ICSI) technique. Oocytes were obtained either by laparoscopic ovum pick-up (LOPU) or by slicing of abattoir ovaries. The donor does used for LOPU were superstimulated and their estrus was synchronized. Recovered oocytes from both sources were cultured in microdrops of IVM medium in the presence of CO2 (5%) at 38.5 °C in a CO2 incubator for 27 h. The ICSI was performed with immobilized sperm on the lid of a culture dish (35 mm) under an …

Fatty Acid Profiles Of Microdiets For Marine Fish In Turkey, Mustafa Yildiz

Turkish Journal of Veterinary & Animal Sciences

Lipid contents and fatty acid profiles in the commercial microdiets for farming marine fish species were determined. Twenty microdiet samples were taken from marine fish farms on the Aegean region of Turkey throughout 2006-2007. Of the 20 microdiet samples, 5 were produced in Turkey and 15 were imported. The fatty acid analysis of the microdiets was made by gas-liquid chromatography using helium as the carrier gas. The lipid levels of the microdiets produced in Turkey by different companies ranged from 9.5 ± 0.05% to 11.6 ± 0.14% (P < 0.05). In contrast, the lipid levels of imported microdiets were between 10.6 ± 0.20% and 17.2 ± 0.14% (P < 0.05). n-3 polyunsaturated fatty acids (n-3 PUFA) levels in the microdiets ranged from 7.1 ± 0.03% to 24.8 ± 0.03% of total fatty acids (P < 0.05). Similarly, n-3 highly unsaturated fatty acid (n-3 HUFA) levels in the microdiets ranged from 6.1 ± 0.01% to 22.8 ± 0.02% of total fatty acids (P < 0.05). n-3 highly unsaturated fatty acids (n-3 HUFA) levels in the microdiets were found between 0.6 ± 0.00% and 3.6 ± 0.01% of dry weight of diets (P < 0.05). Docosahexaenoic acid (DHA) levels of these microdiets were between 0.3 ± 0.00% and 2.0 ± 0.03% of the dry weight of diets (P < 0.05). Eicosapentaenoic acid (EPA) levels in the dry weight of diets were between 0.3 ± 0.01% and 2.2 ± 0.01% (P < 0.05). Arachidonic acid (ArA) levels in the microdiets were also found between 0.3 ± 0.01% and 0.9 ± 0.01% of total fatty acids (P < 0.05). The value of DHA/EPA ratios in the microdiets ranged from 0.5 ± 0.01 to 2.0 ± 0.01 (P > 0.05). The results showed that the total lipid or n-3 and n-6 …

Surgical Correction Of Small Colon Faecalith In A Dare-Shuri Foal, Behrooz Nikahval, Nasser Vesal, Mohsen Ghane

Turkish Journal of Veterinary & Animal Sciences

A 6-month-old male Dare-Shuri foal was admitted for acute abdominal pain unresponsive to medical treatment. The foal was anxious and exhibiting signs of abdominal pain. The foal had not defecated in the previous 5 days and severe abdominal distension was present. Based on clinical signs and the results of the diagnostic work-up, gastrointestinal obstruction was suspected. Midline exploratory celiotomy revealed a faecalith in the small colon. A longitudinal incision was made through the antimesenteric border of the involved intestine and an approximately 350 g mass mostly composed of dried roughage was removed. The day after surgery, intestinal sounds were detectable; …

Study On Eimeria And Cryptosporidium Infections In Sheep And Goats At Elfora Export Abattoir, Debre-Zeit, Ethiopia, Dinka Ayana, Getachew Tilahun, Abebe Wossene

Turkish Journal of Veterinary & Animal Sciences

A cross sectional study on Eimeria and Cryptosporidium infections in small ruminants at ELFORA export abattoir was conducted from October 2005 to February 2006. In this study, attempts were made to determine the prevalence and intensity of Eimeria and Cryptosporidium infections in sheep and goats. Moreover, potential risk factors associated with the infections and the species of Eimeria and Cryptosporidium incriminated in the infections were identified. A total of 384 fecal samples were collected and examined by flotation technique using Sheather's sugar solution to detect the oocysts of Eimeria and Cryptosporiudium species. For Cryptosporidium infection, fecal samples were also examined …

Effects Of Addition Of Vitamin B_{12} To The Extender On Post-Thaw Motility, Acrosome Morphology, And Plasma Membrane Integrity In Bull Semen, Jian Hong Hu, Qing Wang Li, Yu-Lin Chen, Zhong Liang Jiang, Yong Hong Jia, Li Qiang Wang, Bo Bo Ou

Turkish Journal of Veterinary & Animal Sciences

To investigate the effects of supplementation of vitamin B_{12} on bovine sperm post-thaw quality, vitamin B_{12} was added at the concentrations of 1.25, 2.50, 3.75, and 5.00 mg/mL to bovine semen cryoprotective medium. The results indicated that the motility and VSL, VCL, STR, and VAP values of sperm supplemented with 2.50 mg/mL vitamin B_{12} were significantly higher than those of other concentrations (P < 0.05). No significant difference was observed for LIN and ALH values and the percentage of grade a spermatozoa between the extenders containing 2.50 mg/mL and 3.75 mg/mL vitamin B_{12} (P > 0.05). The percentages of acrosome-intact and plasma membrane-intact spermatozoa were significantly improved (P < 0.05) by supplementing with 2.50 mg/mL vitamin B_{12}. With all parameters measured, the concentration of 2.50 mg/mL vitamin B_{12} showed the best results on the quality of bovine semen in freezing-thawing. In conclusion, the addition of 2.50 mg/mL vitamin B_{12} to the extender improved the bovine frozen semen quality.

A Comparison Of The Clinical Effects Associated With Xylazine, Ketamine, And A Xylazine-Ketamine Cocktail In Pigeons (Columba Livia), Uzma Farid Durrani, Muhammad Ashraf, Muhammad A. Khan

Turkish Journal of Veterinary & Animal Sciences

A study was conducted to compare the clinical effects of xylazine, ketamine, and a cocktail of them in pigeons (Columbia livia). For this study 15 adult and healthy pigeons were divided into 3 equal groups: A - xylazine (16 mg/kg), B - ketamine (60 mg/kg) and C - xylazine-ketamine cocktail (8 mg/kg + 30 mg/kg, respectively). All treatments were administered intramuscularly. Onset of action was smooth with a mean time of onset of 13.4 ± 2.78 min, 11 ± 1.49 min, and 1.6 ± 0.51 min, respectively. In group A and B light sedation and a light plane of anaesthesia …

Laparoscopic Sterilization Vs. Open Method Sterilization In Dogs: A Comparison Of Two Techniques, Anburaja Mahalingam, Naveen Kumar, Swapan K. Maiti, Ashok K. Sharma, Umesh Dimri, Meena Kataria

Turkish Journal of Veterinary & Animal Sciences

Ten clinically healthy, adult male dogs randomly equally divided into 2 groups (I and II) were subjected to laparoscopic sterilization and open method castration under xylazine-ketamine anesthesia. In group I laparoscopic vasectomy by cauterization and cutting of the vas deferens was performed and in group II conventional open castration by the pre-scrotal approach was done. Insufflation of the abdominal cavity was achieved with CO_2 (2 L/min) at 10 mmHg pressure gradient. Two ports were needed to carry out the operation. Clinical observations revealed no significant changes. Differential leukocyte count (DLC) revealed significant neutrophilia and comparative lymphopenia on the 3rd postoperative …

Structural Aberration In Ha Associated With Adaptation Of Human Influenza A H3n2 Virus In Embryonated Chicken Eggs, Viroj Wiwanitkit

Turkish Journal of Veterinary & Animal Sciences

Molecular changes associated with adaptation of human influenza A virus in embryonated chicken eggs are reported: 4 amino acid substitutions occurred in HA (G186V, S219F, V226I, V309I). These substitutions allowed binding to SAalpha2,3Gal- and SAalpha2,6Gal-containing receptors, conferred SAalpha2,3Gal specificity, and preserved antigenicity. Here, the author reports the result of protein secondary structure predictions of the adaption from its primary sequence using the NNPREDICT server. Comparing the classical HA of H3N2, the adaptation has additional helices and strands and these identified structural changes should be the explanation for the reported binding and antigenicity changes.

Ileal Leiomyoma In A Captive Zebra (Equus Burchelli), Rahşan Yilmaz, Ahmet Akkoç, M. Özgür Özyi̇ği̇t

Turkish Journal of Veterinary & Animal Sciences

In the present study, an ileal leiomyoma encountered in a captive zebra (Equus burchelli) was reported. A 29-year-old, female zebra was found dead in the Bursa zoo. According to the referring veterinarian the most prominent clinical signs were intermittent colic (responsive to analgesics) and constipation. At post mortem examination, abdomen was severely distended. A firm, multilobular, well vascularized and encapsulated tumoural mass, 12 cm in diameter, embedded into the proximal part of the ileum wall, and protruded from the serosal surface was found. The cut section of the mass had whorled appearance, pale yellow to white in colour, and had …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

The Sheaf Catalogs Of George John Spencer, Larissa C. Brookes

Master's Theses

No abstract provided.

Job Related Stress And The Ability To Transition Among Life Roles For Police Officers, Stacy Dawn Seay

Master's Theses

No abstract provided.

Wintering Duck Response To Trail Use At Former San Francisco Bay Salt Ponds, Heather Renee White

Master's Theses

No abstract provided.

A Pastor's Crucifixion, Tommy Mouton

Master's Theses

No abstract provided.

Regeneration Of Damaged Growth Plate Using Igf-I Plasmid-Releasing Porous Plga Scaffolds, Nirmal Ravi

University of Kentucky Doctoral Dissertations

Growth plate injuries account for 15-30% of long bone fractures in children. About 10% of these result in significant growth disturbances due to formation of a boney bar. If not treated correctly, this can lead to life-lasting consequences of limb length inequalities and angular deformities. Current treatments for growth plate injuries include removal of boney bar and insertion of fat, silicone, bone cement, etc.. This treatment y is inadequate, leaving almost half of these patients with continued deformities. This dissertation reports characterization of a DNA–containing porous poly(lactic-co-glycolic acid) (PLGA) scaffold system, chondrogenesis using insulin-like growth factor I (IGF-I) plasmid-releasing scaffolds …

The Pharmacogenomics Of Egfr-Dependent Nsclc: Predicting And Enhancing Response To Targeted Egfr Therapy, Justin M. Balko

University of Kentucky Doctoral Dissertations

The introduction of tyrosine kinase inhibitors (TKI) targeting the epidermal growth factor receptor (EGFR) inhibitors to the clinic has resulted in an improvement in the treatment of non small cell lung cancer (NSCLC). However, many patients treated with EGFR TKIs do not respond to therapy. The burden of failed treatment is largely placed on the healthcare field, limiting the effectiveness of EGFR TKIs. Furthermore, responses are hindered by the emergence of resistance. Thus, two questions must be addressed to achieve maximum benefit of EGFR inhibitors: How can patients who will benefit from EGFR TKIs be selected a priori? How …

Imide-Functionalized Conjugated Polymers: Synthesis, Structure-Property And Device Studies, Xugang Guo

University of Kentucky Doctoral Dissertations

Organic semiconductors are widely studied as potential active components for consumer electronics due largely to their easily tuned properties and the promise of lower-cost solution-based processing technology. Imide-functionalized organic small molecule compounds have been one of the more important and studied organic semiconductors. However, very few imide-functionalized conjugated polymers have been reported in the literature. The body of this dissertation focuses on the synthesis, structure-property and device studies of imide-functionalized conjugated polymers. Reasons for choosing arylene imides as polymer building blocks include: a) they impart low-lying LUMOs to polymers, allowing band-gap engineering through choice of comonomers with variable electron-donating ability; …

Three Essays On Exchange Rate Economics, Gil Kim

University of Kentucky Doctoral Dissertations

A country’s economy is becoming more and more dynamic and complicated in its scale and mobility. So, the concerns of exchange rate economics have become more popular. My research interest is in international economics with its major factor, exchange rates and other macroeconomic variables. Chapter 1 presents a brief introduction of the three studies.

Chapter Two investigate the role of exchange rate changes with particular attention to international capital flows. With liberalization of capital movements, international capital movements became free and unrestricted in many emerging market economies as well as developed countries. Using a Vector Auto-regressive (VAR) model for a …

The Interplay Between The Expression And Functions Of Wnt13 Isoforms During Apoptosis In Bovine Aortic Endothelial Cells, Tao Tang

University of Kentucky Doctoral Dissertations

Wnt proteins are crucial for development/homeostasis by controlling cell fate including apoptosis (Moon RT et al. 1997). Three humanWnt13 isoforms were identified: the secreted Wnt13A, mitochondrial Wnt13B, and nuclear Wnt13C forms; and nuclear Wnt13 had an increased sensitivity to LPS/TNF-induced apoptosis in primary endothelial cells (EC); both Wnt13B and C mRNA contain two start codons (AUG+1 and +74), but the same protein encoded from AUG+74 by Wnt13C was expressed lower than Wnt13B (Struewing IT et al.2006). We hypothesize that during EC apoptosis, the nuclear Wnt13C expression is regulated translationally; nuclear Wnt13 favors apoptosis through regulating the activity/expression of apoptosis-related factors; …

Production Sequencing And Stability Analysis Of A Just-In-Time System With Sequence Dependent Setups, John Thomas Henninger

University of Kentucky Doctoral Dissertations

Just-In-Time (JIT) production systems is a popular area for researchers but real-world issues such as sequence dependent setups are often overlooked. This research investigates an approach for determining stability and an approach for mixed product sequencing in production systems with sequence dependent setups and buffer thresholds which signal replenishment of a given buffer. Production systems in this research operate under JIT pull production principles by producing only when demand exists and idle when no demand exists.

In the first approach, an iterative method is presented to determine stability for a multi-product production system that operates with replenishment signals and may …