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Sequencing, Analysis, And Annotation Of Expressed Sequence Tags For Camelus Dromedarius, Abdulaziz M. Al-Swailem, Maher M. Shehata, Faisel M. Abu-Duhier, Essam J. Al-Yamani, Khalid A. Al-Busadah, Mohammed S. Al-Arawi, Ali Y. Al-Khider, Abdullah N. Al-Muhaimeed, Fahad H. Al-Qahtani, Manee M. Manee, Badr M. Al-Shomrani, Saad M. Al-Qhtani, Amer S. Al-Harthi, Kadir C. Akdemir, Mehmet S. Inan, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

Despite its economical, cultural, and biological importance, there has not been a large scale sequencing project to date for Camelus dromedarius. With the goal of sequencing complete DNA of the organism, we first established and sequenced camel EST libraries, generating 70,272 reads. Following trimming, chimera check, repeat masking, cluster and assembly, we obtained 23,602 putative gene sequences, out of which over 4,500 potentially novel or fast evolving gene sequences do not carry any homology to other available genomes. Functional annotation of sequences with similarities in nucleotide and protein databases has been obtained using Gene Ontology classification. Comparison to available …

Genes Differentially Expressed By Cumulus Cells And Assays Using Same To Identify Pregnancy Competent Oocytes, Jose B. Cibelli, Amy E. Iager, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

A genetic means of identifying "pregnancy competent" oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the "pregnancy signature", also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. In preferred embodiments the …

Family Legacies: Constructing Individual And Family Identity Through Intergenerational Storytelling, Blair Thompson, Jody Koenig Kellas, Jordan Soliz, Jason Thompson, Amber Epp, Paul Schrodt

Department of Communication Studies: Faculty Publications

The current study focused on discovering the ways in which the intergenerational transmission of family legacy stories both enables and constrains individual family members’ sense of their own identities. Using semistructured interviews, 17 third-generation family members identified a multitude of both positive and negative family legacies. Both positive and negative legacies were influenced by the storytelling context. Positive legacies portrayed families as hardworking, caring, and cohesive while negative legacies were more idiosyncratic. Individual family members typically responded to their family legacies by embracing the positive and rejecting the negative. However, individuals’ responses also pointed to additional complexities in accepting or …

Genomic Instability In Regions Adjacent To A Highly Conserved Pch Prophage In Escherichia Coli O157:H7 Generates Diversity In Expression Patterns Of The Lee Pathogenicity Island, Zhijie Yang, Jaehyoung Kim, Chaomei Zhang, Min Zhang, Joeseph Nietfeldt, Carolyn M. Southward, Michael G. Surette, Stephen D. Kachman, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

The LEE pathogenicity island has been acquired on multiple occasions within the different lineages of

enteropathogenic and enterohemorrhagic Escherichia coli. In each lineage, LEE expression is regulated by complex networks of pathways, including core pathways shared by all lineages and lineage-specific pathways. Within the O157:H7 lineage of enterohemorrhagic E. coli, strain-to-strain variation in LEE expression has been observed, implying that expression patterns can diversify even within highly related subpopulations. Using comparative genomics of E. coli O157:H7 subpopulations, we have identified one source of strain-level variation affecting LEE expression. The variation occurs in prophage-dense regions of the genome that …

Pyramidobacter Piscolens Gen. Nov., Sp. Nov., A Member Of The Phylum ‘Synergistetes’ Isolated From The Human Oral Cavity, Julia Downes, Sonia R. Vartoukian, Floyd E. Dewhirst, Jacques Izard, Tsute Chen, Wen-Han Yu, Iain C. Sutcliffe, William G. Wade

Department of Food Science and Technology: Faculty Publications

Four strains of anaerobic, gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group distinct from any species with validly published names. 16S rRNA and 23S rRNA gene sequence analyses and DNA–DNA reassociation data revealed that the strains constituted a novel group within the phylum ‘Synergistetes’ and were most closely related to Jonquetella anthropi. Two libraries of randomly cloned DNA were prepared from strain W5455T and were sequenced to provide a genome survey as a resource for metagenomic studies. A new genus …

Cryo-Electron Tomography Elucidates The Molecular Architecture Of Treponema Pallidum, The Syphilis Spirochete, Jacques Izard, Christian Renken, Chyong-Ere Hsieh, Daniel C. Desrosiers, Star Dunham-Ems, Carson La Vake, Linda L. Gebhardt, Ronald J. Limberger, David L. Cox, Michael Marko, Justin D. Radolf

Department of Food Science and Technology: Faculty Publications

Cryo-electron tomography (CET) was used to examine the native cellular organization of Treponema pallidum, the syphilis spirochete. T. pallidum cells appeared to form flat waves, did not contain an outer coat and, except for bulges over the basal bodies and widening in the vicinity of flagellar filaments, displayed a uniform periplasmic space. Although the outer membrane (OM) generally was smooth in contour, OM extrusions and blebs frequently were observed, highlighting the structure’s fluidity and lack of attachment to underlying periplasmic constituents. Cytoplasmic filaments converged from their attachment points opposite the basal bodies to form arrays that ran roughly …

Alcohol Expectancies Among High School Students In Inner Mongolia, China, Duane F. Shell, Ian Newman, Ming Qu

Department of Educational Psychology: Faculty Publications

Objective — This study examines differences in Chinese high school students’ alcohol expectancies by drinking status (nondrinker, occasional drinker, regular drinker) and gender (male, female).
Method — The authors administered the Chinese Adolescent Alcohol Expectancy Questionnaire (CAEQ) to a convenience sample of 1244 high school students (M = 627; F = 617) from schools in Hohhot City and Tongliao City in Inner Mongolia, China.
Results — Differences were found in the 8 CAEQ factors (3 negative and 5 positive factors). Regular drinkers had lower negative consequences and higher positive perception expectancies than nondrinkers or occasional drinkers. Nondrinkers had higher harm …

Teacher–Child Relationship Quality: The Roles Of Child Temperament And Teacher–Child Interactions, Kathleen Moritz Rudasill, Sara E. Rimm-Kaufman

Department of Educational Psychology: Faculty Publications

Young children’s relationships with teachers predict social and academic success. This study examines contributions of child temperament (shyness, effortful control) and gender to teacher–child relationship quality both directly and indirectly through the frequency of teacher–child interactions in the classroom. Using an NICHD SECCYD sample of 819 first grade children, four findings emerged: (a) children’s shyness, effortful control, and gender contributed directly to teacher–child conflict and closeness; (b) children’s shyness contributed to the frequency of child-initiated teacher–child interactions, and children’s effortful control contributed to the frequency of teacher-initiated teacher–child interactions; (c) shyness related to teacher–child closeness indirectly through the frequency of …

Parent Involvement As A Predictor Of Teacher–Child Relationship Quality In Third Grade, Amanda J. Wyrick, Kathleen Moritz Rudasill

Department of Educational Psychology: Faculty Publications

Research Findings: Research on teacher–child relationships is important, as the quality of this relationship is linked to numerous child outcomes in the areas of academic and social functioning. In addition, parent involvement has been identified as a significant factor in the successful development of a child. This study attempted to join these two lines of research by assessing the extent to which teacher–child relationship quality varies as a function of parent involvement. We used a sample of 894 third-grade children, mothers, and teachers from the National Institute of Child Health and Human Development (NICHD) Study of Early Child Care. Regression …

Predatory Senescence In Ageing Wolves, Daniel R. Macnulty, Douglas W. Smith, John A. Vucetich, L. David Mech, Craig Packer

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

It is well established that ageing handicaps the ability of prey to escape predators, yet surprisingly little is known about how ageing affects the ability of predators to catch prey. Research into long-lived predators has assumed that adults have uniform impacts on prey regardless of age. Here we use longitudinal data from repeated observations of individually-known wolves (Canis lupus) hunting elk (Cervus elaphus) in Yellowstone National Park to demonstrate that adult predatory performance declines with age and that an increasing ratio of senescent individuals in the wolf population depresses the rate of prey offtake. Because this ratio fluctuates independently of …

Problems With The Claim Of Ecotype And Taxon Status Of The Wolf In The Great Lakes Region, Matthew A. Cronin, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Koblmuller et al. (2009) analyzed molecular genetic data of the wolf in the Great Lakes (GL) region of the USA and concluded that the animal was a unique ecotype of grey wolf and that genetic data supported the population as a discrete wolf taxon. However, some of the literature that the researchers used to support their position actually did not, and additional confusion arises from indefinite use of terminology. Herein, we discuss the problems with designation of a wolf population as a taxon or ecotype without proper definition and assessment of criteria.

Koblmuller et al. (2009) wrote ‘The GL …

A Serological Survey Of Infectious Disease In Yellowstone National Park’S Canid Community, Emily S. Almberg, L. David Mech, Douglas W. Smith, Jennifer W. Sheldon, Robert L. Crabtree

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Background: Gray wolves (Canis lupus) were reintroduced into Yellowstone National Park (YNP) after a .70 year absence, and as part of recovery efforts, the population has been closely monitored. In 1999 and 2005, pup survival was significantly reduced, suggestive of disease outbreaks.

Methodology/Principal Findings: We analyzed sympatric wolf, coyote (Canis latrans), and red fox (Vulpes vulpes) serologic data from YNP, spanning 1991–2007, to identify long-term patterns of pathogen exposure, identify associated risk factors, and examine evidence for disease-induced mortality among wolves for which there were survival data. We found high, constant exposure to canine …

Characterization Of A Monothiol Glutaredoxin Encoded By Chlorella Virus Pbcv-1, Lisa A. Fitzgerald, Yuanzheng Zhang, Gentry L. Lewis, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Annotation of the 330-kb Chlorella virus PBCV-1 genome identified a 237 nucleotide gene (a438l) that codes for a protein with ~35% amino acid identity to glutaredoxins (Grx) found in other organisms. The PBCV-1 protein resembles classical Grxs in both size (9 kDa) and location of the active site (N-terminus). However, the PBCV-1 Grx is unusual because it contains a monothiol active site (CPYS) rather than the typical dithiol active site (CPYC). To examine this unique active site, four sitespecific mutants (CPYC, CPYA, SPYC, and SPYS) were constructed to determine if the N-terminal cysteine is necessary for enzyme activity. …

The Zinc Finger Dna-Binding Domain Of K-Rbp Plays An Important Role In Regulating Kaposi’S Sarcoma-Associated Herpesvirus Rta-Mediated Gene Expression, Zhilong Yang, Hui-Ju Wen, Veenu Minhas, Charles Wood

Nebraska Center for Virology: Faculty Publications

K-RBP is a KRAB-containing zinc finger protein with multiple zinc finger motifs and represses Kaposi’s sarcoma-associated herpesvirus (KSHV) transactivator RTA-mediated transactivation of several viral lytic gene promoters, including the ORF57 promoter. Whether K-RBP binds DNA through its zinc fingers and the role of zinc finger domain in repressing gene expression are unclear. Here we report that K-RBP binds DNA through its zinc finger domain and the target DNA sequences contain high GC content. Furthermore, K-RBP binds to KSHV ORF57 promoter, which contains a GC-rich motif. K-RBP suppresses the basal ORF57 promoter activity as well as RTA-mediated activation. The zinc finger …

Type I Interferons And Interferon Regulatory Factors Regulate Tnf-Related Apoptosis-Inducing Ligand (Trail) In Hiv-1-Infected Macrophages, Yunlong Huang, Angelique Walstrom, Luwen Zhang, Yong Zhao, Min Cui, Ling Ye, Jialin C. Zheng

Nebraska Center for Virology: Faculty Publications

TNF-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family that participates in HIV-1 pathogenesis through the depletion of CD4+ T cells. TRAIL is expressed on the cell membrane of peripheral immune cells and can be cleaved into a soluble, secreted form. The regulation of TRAIL in macrophages during HIV-1 infection is not completely understood. In this study, we investigated the mechanism(s) of TRAIL expression in HIV-1-infected macrophages, an important cell type in HIV-1 pathogenesis. A human monocyte-derived macrophage (MDM) culture system was infected with macrophage-tropic HIV-1ADA, HIV-1JR-FL, or HIV-1BAL strains. TRAIL, predominantly the …

Human Ubc9 Contributes To Production Of Fully Infectious Human Immunodeficiency Virus Type 1 Virions, Tareq Jaber, Christopher R. Bohl, Gentry L. Lewis, Charles Wood, John T. West Jr., Robert A. Weldon Jr.

Nebraska Center for Virology: Faculty Publications

Ubc9 was identified as a cellular protein that interacts with the Gag protein of Mason-Pfizer monkey virus. We show here that Ubc9 also interacts with the human immunodeficiency virus type 1 (HIV-1) Gag protein and that their interaction is important for virus replication. Gag was found to colocalize with Ubc9 predominantly at perinuclear puncta. While cells in which Ubc9 expression was suppressed with RNA interference produced normal numbers of virions, these particles were 8- to 10-fold less infectious than those produced in the presence of Ubc9. The nature of this defect was assayed for dependence on Ubc9 during viral assembly, …

Expression Of Hpv16 E5 Produces Enlarged Nuclei And Polyploidy Through Endoreplication, Lulin Hu, Tamara A. Potapova, Shibo Li, Susannah Rankin, Gary J. Gorbsky, Peter C. Angeletti, Brian P. Ceresa

Nebraska Center for Virology: Faculty Publications

Anogenital cancers and head and neck cancers are causally-associated with infection by high-risk

human papillomavirus (HPV). The mechanism by which high-risk HPVs contribute to

oncogenesis is poorly understood. HPV16 encodes three genes (HPV16 E5, E6, and E7) that can

transform cells when expressed independently. HPV16 E6 and E7 have well-described roles

causing genomic instability and unregulated cell cycle progression. The role of HPV16 E5 in cell

transformation remains to be elucidated. Expression of HPV16 E5 results in enlarged, polyploid

nuclei that are dependent on the level and duration of HPV16 E5 expression. Live-cell imaging

data indicate these changes do not …

Human Papillomavirus 16 E5 Induces Bi-Nucleated Cell Formation By Cell-Cell Fusion, Lulin Ha, Kendra Plafker, Valeriya Vorozhoko, Rosemary E. Zuna, Marie H. Hanigan, Gary J. Gorbsky, Scott M. Plafker, Peter C. Angeletti, Brian P. Ceresa

Nebraska Center for Virology: Faculty Publications

Human Papillomaviruses (HPV) 16 is a DNA virus encoding three oncogenes – E5, E6, and E7. The E6 and E7 proteins have well-established roles as inhibitors of tumor suppression, but the contribution of E5 to malignant transformation is controversial. Using spontaneously immortalized human keratinocytes (HaCaT cells), we demonstrate that expression of HPV16 E5 is necessary and sufficient for the formation of bi-nucleated cells, a common characteristic of precancerous cervical lesions. Expression of E5 from non-carcinogenic HPV6b does not produce bi-nucleate cells. Video microscopy and biochemical analyses reveal that bi-nucleates arise through cell-cell fusion. Although most E5-induced bi-nucleates fail to propagate, …

A Comparative Study Of Hiv-1 Clade C Env Evolution In A Zambian Infant With An Infected Rhesus Macaque During Disease Progression, For Yue Tso, Federico G. Hoffmann, Damien C. Tully, Philippe Lemey, Robert A. Rasmussen, Hong Zhang, Ruth M. Ruprecht, Charles Wood

Nebraska Center for Virology: Faculty Publications

Objective—To evaluate whether HIV-1 clade C (HIV-C) envelope variations that arise during disease progression in rhesus macaque model reflect changes that occur naturally in human infection.

Design—An infant macaque was infected with SHIV-1157i, an R5 tropic clade C SHIV (SHIV-C) which expresses a primary HIV-C envelope derived from an infected human infant, and monitored over a five-year period. Genetic variation of the V1-V5 envelope region, which is the main target for humoral immune responses, derived from the infected macaque and infant was examined.

Methods—V1-V5 envelope region were cloned and sequenced from longitudinal PBMC samples collected from the infected macaque and …

Protection Against Mucosal Shiv Challenge By Peptide And Helper-Dependent Adenovirus Vaccines, Eric A. Weaver, Pramod N. Nehete, Bharti P. Nehete, Stephanie J. Buchl, Donna Palmer, David C. Montefiori, Philip Ng, K. Jagannadha Sastry, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Groups of rhesus macaques that had previously been immunized with HIV-1 envelope (env) peptides and first generation adenovirus serotype 5 (FG-Ad5) vaccines expressing the same peptides were immunized intramuscularly three times with helper- dependent adenovirus (HD-Ad) vaccines expressing only the HIV-1 envelope from JRFL. No gag, pol, or other SHIV genes were used for vaccination. One group of the FG-Ad5- immune animals was immunized three times with HD-Ad5 expressing env. One group was immunized by serotype-switching with HD-Ad6, HD-Ad1, and HD-Ad2 expressing env. Previous work demonstrated that serum antibody levels against env were significantly higher in the serotype-switched group than …

Feeding Ecology Of Arctic-Nesting Sandpipers During Spring Migration Through The Prairie Pothole Region, Jan Eldridge, Gary Krapu, Douglas Johnson

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We evaluated food habits of 4 species of spring-migrant calidrid sandpipers in the Prairie Pothole Region (PPR) of North Dakota. Sandpipers foraged in several wetland classes and fed primarily on aquatic dipterans, mostly larvae, and the midge family Chironomidae was the primary food eaten. Larger sandpiper species foraged in deeper water and took larger larvae than did smaller sandpipers. The diverse wetland habitats that migrant shorebirds use in the PPR suggest a landscape-level approach be applied to wetland conservation efforts. We recommend that managers use livestock grazing and other tools, where applicable, to keep shallow, freshwater wetlands from becoming choked …

Missouri River Recovery Program: Missouri River Basin

United States Army Corps of Engineers, Omaha District

The Missouri River, the nation’s longest, travels more than 2,300 miles from Three Forks, Montana, to join the Mississippi River near St. Louis, Missouri. For centuries, the river has served as the center of life for the inhabitants of North America’s Great Plains. Following exploration and mapping by Lewis and Clark’s Corps of Discovery in the early 1800s, the Missouri River became the gateway to the West.

Historically, the dynamic and untamed Missouri River has produced violent floods in the spring and summer with erosive forces that ate away its banks and turned its waters the color of mud.

Since …

Missouri River Recovery Program: Emergent Sandbar Habitat

United States Army Corps of Engineers, Omaha District

This Value Engineering (VE) Report summarizes the events of the April 6-8, 2009 VE workshop facilitated by GeoVal, Inc. for the U.S. Army Corps of Engineers (USACE) Omaha District, Nebraska. The Missouri River Recovery Program - Emergent Sandbar Habitat Value Engineering Study focuses on the assessment of the Missouri River Recovery Program (MRRP) Habitat Creation Project with specific focus on the creation of emergent sandbar habitats, examining current plans and programs to seek out alternative approaches and ideas that will improve the overall performance of the program.

The MRRP seeks to mitigate near-term losses of Missouri River habitats and recover …

Missouri River Recovery Program: Putting The Pieces Together: Mrrp Main Programs

United States Army Corps of Engineers, Omaha District

The Missouri River Recovery Program (MRRP) is a comprehensive effort led by the U.S. Army Corps of Engineers in partnership with the U.S. Fish and Wildlife Service, Tribes, states, other federal agencies and non-governmental organizations to develop and implement actions to create a healthy Missouri River.

The MRRP consists of:

-The Missouri River Bank Stabilization and Navigation Project (BSNP) Fish
and Wildlife Mitigation Project

- The Amended Biological Opinion and the Corps’ Record of Decision related
to the Master Water Control Manual for the Missouri River

- The 2007 Water Resources Development Act

Each of these guiding authorities set out …

Body Size And Predatory Performance In Wolves: Is Bigger Better?, Daniel R. Macnulty, Douglas W. Smith, L. David Mech, Lynn E. Eberly

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Summary

1. Large body size hinders locomotor performance in ways that may lead to trade-offs in predator foraging ability that limit the net predatory benefit of larger size. For example, size-related improvements in handling prey may come at the expense of pursuing prey and thus negate any enhancement in overall predatory performance due to increasing size.

2. This hypothesis was tested with longitudinal data from repeated observations of 94 individually known wolves (Canis lupus) hunting elk (Cervus elaphus) in Yellowstone National Park, USA. Wolf size was estimated from an individually based sex-specific growth model derived from …

Wolf Use Of Summer Territory In Northeastern Minnesota, Dominic J. Demma, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Movements of wolves (Canis lupus) during summer 2003 and 2004 in the Superior National Forest were based around homesites but included extensive use of territories. Away from homesites, wolves used different areas daily, exhibiting rotational use. Mean daily range overlap was 22% (SE = 0.02) and that of breeding wolves was significantly greater than for nonbreeders (x = 25% and 16%, respectively). Rotational use may improve hunting success. Managers seeking to remove entire packs must maintain control long enough to ensure that all pack members are targeted.

Vulnerability Of Rehabilitated Agricultural Production Systems To Invasion By Nontarget Plant Species, Sara G. Baer, David M. Engle, Johannes M. H. Knops, Kenneth A. Langeland, Bruce D. Maxwell, Fabian D. Menalled, Amy J. Symstad

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Vast areas of arable land have been retired from crop production and ‘‘rehabilitated’’ to improved system states through landowner incentive programs in the United States (e.g., Conservation and Wetland Reserve Programs), as well as Europe (i.e., Agri-Environment Schemes). Our review of studies conducted on invasion of rehabilitated agricultural production systems by nontarget species elucidates several factors that may increase the vulnerability of these systems to invasion. These systems often exist in highly fragmented and agriculturally dominated landscapes, where propagule sources of target species for colonization may be limited, and are established under conditions where legacies of past disturbance persist and …

Characterization Of The 5′- And 3′-Terminal Subgenomic Rnas Produced By A Capillovirus: Evidence For A Cp Subgenomic Rna, Satyanarayana Tatineni, Mohammad R. Afunian, Siddarame Gowda, Mark E. Hilf, Moshe Bar-Joseph, William O. Dawson

Department of Plant Pathology: Faculty Publications

The members of Capillovirus genus encode two overlapping open reading frames (ORFs): ORF1 encodes a large polyprotein containing the replication-associated proteins plus a coat protein (CP), and ORF2 encodes a movement protein (MP), located within ORF1 in a different reading frame. Organization of the CP sequence as part of the replicase ORF is unusual in capilloviruses. In this study, we examined the capillovirus genome expression strategy by characterizing viral RNAs produced by Citrus tatter leaf virus (CTLV), isolate ML, a Capillovirus. CTLV-ML produced a genome-length RNA of ∼6.5-kb and two 3′-terminal sgRNAs in infected tissue that contain the MP …

Chlorella Virus Atcv-1 Encodes A Functional Potassium Channel Of 82 Amino Acids, Sabrina Gazzarrini, Ming Kang, Alessandra Abenavoli, Giulia Romani, Claudio Olivari, Daniele Gaslini, Giuseppina Ferrara, James L. Van Etten, Michael Kreim, Stefan M. Kast, Gerhard Thiel, Anna Moroni

Department of Plant Pathology: Faculty Publications

Chlorella virus PBCV-1 (Paramecium bursaria chlorella virus-1) encodes the smallest protein (94 amino acids, named Kcv) previously known to form a functional K+ channel in heterologous systems. In this paper, we characterize another chlorella virus encoded K+ channel protein (82 amino acids, named ATCV-1 Kcv) that forms a functional channel in Xenopus oocytes and rescues Saccharomyces cerevisiae mutants that lack endogenous K+ uptake systems. Compared with the larger PBCV-1 Kcv, ATCV-1 Kcv lacks a cytoplasmic N-terminus and has a reduced number of charged amino acids in its turret domain. Despite these deficiencies, ATCV-1 Kcv accomplishes all the major features of …

The Phycodnaviridae: The Story Of How Tiny Giants Rule The World, W. H. Wilson, James L. Van Etten, M. J. Allen

Department of Plant Pathology: Faculty Publications

The family Phycodnaviridae encompasses a diverse and rapidly expanding collection of large icosahedral, dsDNA viruses that infect algae. These lytic and lysogenic viruses have genomes ranging from 160 to 560 kb. The family consists of six genera based initially on host range and supported by sequence comparisons. The family is monophyletic with branches for each genus, but the phycodnaviruses have evolutionary roots that connect them with several other families of large DNA viruses, referred to as the nucleocytoplasmic large DNA viruses (NCLDV).The phycodnaviruses have diverse genome structures, some with large regions of noncoding sequence and others with regions of ssDNA. …