Digital Commons Network^™

Coal Dust Alters Β-Naphthoflavone-Induced Aryl Hydrocarbon Receptor Nuclear Translocation In Alveolar Type Ii Cells, Mohamed M. Ghanem, Lori A. Battelli, Brandon F. Law, Vincent Castranova, Michael L. Kashon, Joginder Nath, Ann F. Hubbs

Faculty & Staff Scholarship

Background

Many polycyclic aromatic hydrocarbons (PAHs) can cause DNA adducts and initiate carcinogenesis. Mixed exposures to coal dust (CD) and PAHs are common in occupational settings. In the CD and PAH-exposed lung, CD increases apoptosis and causes alveolar type II (AT-II) cell hyperplasia but reduces CYP1A1 induction. Inflammation, but not apoptosis, appears etiologically associated with reduced CYP1A1 induction in this mixed exposure model. Many AT-II cells in the CD-exposed lungs have no detectable CYP1A1 induction after PAH exposure. Although AT-II cells are a small subfraction of lung cells, they are believed to be a potential progenitor cell for some lung …

Iron Oxide Nanoparticles Induce Human Microvascular Endothelial Cell Permeability Through Reactive Oxygen Species Production And Microtubule Remodeling, Patrick L. Apopa, Yong Qian, Rong Shao, Nancy L. Guo, Diane Schwegler-Berry, Maricica Pacurari, Dale Porter, Xianglin Shi, Val Vallyathan, Vincent Castranova, Daniel C. Flynn

Faculty & Staff Scholarship

Background

Engineered iron nanoparticles are being explored for the development of biomedical applications and many other industry purposes. However, to date little is known concerning the precise mechanisms of translocation of iron nanoparticles into targeted tissues and organs from blood circulation, as well as the underlying implications of potential harmful health effects in human.

Results

The confocal microscopy imaging analysis demonstrates that exposure to engineered iron nanoparticles induces an increase in cell permeability in human microvascular endothelial cells. Our studies further reveal iron nanoparticles enhance the permeability through the production of reactive oxygen species (ROS) and the stabilization of microtubules. …

An Empirical Bayesian Approach For Model-Based Inference Of Cellular Signaling Networks, David J. Klinke Ii

Faculty & Staff Scholarship

Background

A common challenge in systems biology is to infer mechanistic descriptions of biological process given limited observations of a biological system. Mathematical models are frequently used to represent a belief about the causal relationships among proteins within a signaling network. Bayesian methods provide an attractive framework for inferring the validity of those beliefs in the context of the available data. However, efficient sampling of high-dimensional parameter space and appropriate convergence criteria provide barriers for implementing an empirical Bayesian approach. The objective of this study was to apply an Adaptive Markov chain Monte Carlo technique to a typical study of …

X Chromosomes Aneuploidy Lymphocytes Centromeres Chromosomal Translocations Sex Chromosomes Etiology Fluorescent In Situ Hybridiz, Beth M. Cleveland, Stephen S. Leonard, Hillar Klandorf, Kenneth P. Blemings

Faculty & Staff Scholarship

Humans, birds, and some primates do not express the uric acid degrading enzyme urate oxidase (UOX) and, as a result, have plasma uric acid concentrations higher than UOX expressing animals. Although high uric acid concentrations are suggested to increase the antioxidant defense system and provide a health advantage to animals without UOX, knockout mice lacking UOX develop pathological complications including gout and kidney failure. As an alternative to the knockout model, RNA interference was used to decrease UOX expression using stable transfection in a mouse hepatic cell line (ATCC, FL83B). Urate oxidase mRNA was reduced 66% (p < 0.05) compared to wild type, as measured by real time RT-PCR. To determine if UOX knockdown resulted in enhanced protection against oxidative stress, cells were challenged with hexavalent chromium (Cr(VI)) or 3-morpholinosydnonimine hydrochloride (SIN-1). Compared to wild type, cells with UOX knockdown exhibited a 37.2 ± 3.5% reduction (p < 0.05) in the electron spin resonance (ESR) signal after being exposed to Cr(VI) and displayed less DNA fragmentation (p < 0.05) following SIN-1 treatment. Cell viability decreased in wild type cells (p < 0.05), but not cells with UOX knockdown, after treatment with SIN-1. These results are consistent with an increased intracellular uric acid concentration and an increased defense against oxidative stress.

Size And Distribution Of Blue Crabs (Callinectes Sapidus) With Regard To Salinity In The Upper Nueces Estuary, Texas, Matthew D. Schweitzer, Kim Withers

Gulf of Mexico Science

No abstract provided.

In Memoriam: Death Of Dr. Rezneat Darnell, Texas A&M Oceanographer And Ecologist

Gulf of Mexico Science

No abstract provided.

Aggressive Response Of Adult Bobolinks To Neck Ligatures On Nestlings, Lynn P. Little, Allan M. Strong, Noah G. Perlut

Environmental Studies Faculty Publications

We monitored provisioning behavior at 18 Bobolink (Dolichonyx oryzivorus) nests during 240.5 min of videotape data from June to July 2006, and observed 64 nest visits by adults while nestlings were fitted with neck ligatures. Adults pecked or pulled at the ligatures, often aggressively, at 72% of nests (n = 18) and 52% of visits (n = 64). These behavioral responses by adults indicate the neck ligature technique is more invasive than previously believed. We documented no mortality as a result of ligature placement, but researchers should minimize the time that ligatures are in place to reduce stress …

The Pic Protease Of Enteroaggregative Escherichia Coli Promotes Intestinal Colonization And Growth In The Presence Of Mucin, Susan M. Harrington, Jalaluddin Sheikh, Ian R. Henderson, Fernando Ruiz-Perez, Paul S. Cohen, James P. Nataro

Cell and Molecular Biology Faculty Publications

Enteroaggregative Escherichia coli (EAEC) is increasingly being recognized as a cause of diarrheal disease in diverse populations. No small animal model is currently available to study this pathogen. We report here that conventional mice orally inoculated with prototype EAEC strain 042 generally became colonized, though the abundance of organisms cultured from their stool varied substantially among individual animals. In contrast, mice whose water contained 5 g/liter streptomycin consistently became colonized at high levels (ca. 10⁸ CFU/g of stool). Neither conventional nor streptomycin-treated mice developed clinical signs or histopathologic abnormalities. Using specific mutants in competition with the wild-type strain, we …

Novel Features Of The Polysaccharide-Digesting Gliding Bacterium Flavobacterium Johnsoniae As Revealed By Genome Sequence Analysis, Mark J. Mcbride, Gary Xie, Eric C. Martens, Alla Lapidus, Bernard Henrissat, Ryan G. Rhodes, Eugene Goltsman, Wei Wang, Jian Xu, David W. Hunnicutt, Andrew M. Staroscik, Timothy R. Hoover, Yi-Qiang Cheng, Jennifer L. Stein

Cell and Molecular Biology Faculty Publications

The 6.10-Mb genome sequence of the aerobic chitin-digesting gliding bacterium Flavobacterium johnsoniae (phylum Bacteroidetes) is presented. F. johnsoniae is a model organism for studies of bacteroidete gliding motility, gene regulation, and biochemistry. The mechanism of F. johnsoniae gliding is novel, and genome analysis confirms that it does not involve well-studied motility organelles, such as flagella or type IV pili. The motility machinery is composed of Gld proteins in the cell envelope that are thought to comprise the “motor” and SprB, which is thought to function as a cell surface adhesin that is propelled by the motor. Analysis of the …

Direct And Specific Inactivation Of Protein Tyrosine Kinases In The Src And Fgfr Families By Reversible Cysteine Oxidation, David J. Kemble, Gongqin Sun

Cell and Molecular Biology Faculty Publications

Accumulating evidence suggests that protein tyrosine phosphorylation-based signaling pathways are under the regulation of reactive oxygen species. Although protein tyrosine phosphatases are directly regulated by reversible oxidation, it is not clear whether protein tyrosine kinases (PTKs) are also directly regulated by reduction/oxidation (redox). In this study we report a mechanism of direct oxidative inactivation specific for the PTKs in the Src and fibroblast growth factor receptor (FGFR) families, key enzymes in mammalian signal transduction. Src is fully active when reduced and retains 8–25% of the full activity toward various substrates when oxidized. This inactivation is caused by oxidation of a …

Understanding The Physical Properties That Control Protein Crystallization By Analysis Of Largescale Experimental Data, W. Nicholson Price Ii, Yang Chen, Samuel K. Handelman, Helen Neely, Philip Manor, Richard Karlin, Rajesh Nair, Jinfeng Liu, Michael Baran, John Everett, Saichiu N. Tong, Farhad Forouhar, Swarup S. Swaminathan, Thomas Acton, Rong Xiao, Joseph R. Luft, Angela Lauricella, George T. Detitta, Burkhard Rost, Gaetano T. Montelione, John T. Hunt

Law Faculty Scholarship

Crystallization is the most serious bottleneck in high-throughput protein-structure determination by diffraction methods. We have used data mining of the large-scale experimental results of the Northeast Structural Genomics Consortium and experimental folding studies to characterize the biophysical properties that control protein crystallization. This analysis leads to the conclusion that crystallization propensity depends primarily on the prevalence of well-ordered surface epitopes capable of mediating interprotein interactions and is not strongly influenced by overall thermodynamic stability. We identify specific sequence features that correlate with crystallization propensity and that can be used to estimate the crystallization probability of a given construct. Analyses of …

Evaluation Of Silica Monoliths In Affinity Microcolumns For High-Throughput Analysis Of Drug–Protein Interactions, Michelle J. Yoo, David S. Hage

David Hage Publications

Silica monoliths in affinity microcolumns were tested for the high-throughput analysis of drug–protein interactions. HSA was used as a model protein for this work, while carbamazepine and R-warfarin were used as model analytes. A comparison of HSA silica monoliths of various lengths indicated columns as short as 1 to 3 mm could be used to provide reproducible estimates of retention factors or plate heights. Benefits of using smaller columns for this work included the lower retention times and lower back pressures that could be obtained versus traditional HPLC affinity columns, as well as the smaller amount of protein that …

Effectiveness Of Thermal Oxidation In Relation To Anterior Cervical Plates, Katherine A. Miyashiro

Master's Theses

Ti-6Al-4V anterior cervical plates (ACP) are used in spinal fusion surgeries to fixate cervical vertebrae during graft adhesion. However, documented cases of implant failure and the potential for ACP corrosion raise concerns regarding any degradation of material resulting from extended implantation. In addition, abrasion during implantation may damage a section of the protective oxide layer, potentially exposing surrounding tissues to the harmful effects of bare titanium, aluminum, and vanadium. Thermal oxidation has been shown to improve corrosion-resistance and wear-resistance, depending on temperature and time. To quantify the attributes of the thermally grown oxide layer, Ti-6Al-4V coupons underwent thermal oxidation treatments …

Evaluation Of Total Runoff For The Rio San Pedro Sub-Basin (Nayarit, Mexico) Assessing Their Hydrologic Response Units

Journal of Spatial Hydrology

The Rio San Pedro sub-basin runoff was estimated using the curve number method (NRCS-CN) applied to hydrologic response units (HRU’s), derived from remote sensing and GIS analysis. The sub-basin (around 2900 km²) was delineated from digital elevation models (DEM), that also were used to obtain the slopes in the study area. A landscape characterization (overall accuracy > 80%), based on Landsat ETM+ imagery, was obtained using standard classification methods, and together with a rainfall data series, were the input information for the sub-basin discretization in HRU’s and the runoff calculation. Seventeen HRU’s were obtained, that can be arranged in …

Public History Newsletter Winter 2009, Public History Concentration

Public History Newsletter

A four page newsletter created by the Public History Concentration at Wright State University.

Purification And Characterization Of A Novel Salivary Antimicrobial Peptide From The Tick, Ixodes Scapularis, Sivakamasundari Pichu, José M.C. Ribeiro, Thomas N. Mather

Plant Sciences and Entomology Faculty Publications

A novel antimicrobial peptide was isolated from the saliva of the Lyme disease tick vector, Ixodes scapularis, henceforth designated as ISAMP (I. scapularis Antimicrobial Peptide). ISAMP was purified using a sequential method including ultra filtration, gel filtration and reverse-phase high performance liquid chromatography. The purified peak had a molecular weight of 5.3 kDa by MALDI/TOF-MS and its amino acid sequence, determined by Edman degradation was PDxGxPxxVKAGRxPxxSI. A BLASTP search revealed that the protein is a putative 5.3 kDa secreted protein (AAM93656) from I. scapularis. The predicted protein is composed of 69 amino acids with no conserved …

Focus Emu, October 6, 2009, Office Of Public Information

Focus EMU

No abstract provided.

Morphological Characteristics Of Early Life History Stages Of The Blue Crab, Callinectes Sapidus Rathbun, From The Northern Gulf Of Mexico With A Comparison Of Studies From The Atlantic Seaboard, Kenneth Stuck, Harriet Perry, Darcie Graham, Richard W. Heard

Gulf and Caribbean Research

Zoeae, megalopae, and early crab stages of Callinectes sapidus Rathbun, 1896 were described from the northern Gulf of Mexico (nGOM). Observations during this study were based on larvae reared in the laboratory through the early crab stages and on megalopae and early crab stages collected in the wild. Gulf of Mexico data are compared with similar information for the southeast Atlantic coast of the United States. Size and setation of C. sapidus larvae reared from nGOM stocks were different than those in published descriptions of larvae reared from Atlantic populations. Seasonal differences in size were noted in both reared and …

Note On The Natural And Cultural History Of Hurricane Balls, Joyce M. Shaw

Gulf and Caribbean Research

Hurricane balls are natural phenomena of tropical storms and hurricane winds and waves and are found along the shoreline. Gunter Library at the Gulf Coast Research Laboratory (GCRL) is home to a small collection of hurricane balls which were found along the shore lines of barrier islands and marsh beaches by GCRL staff over the years. Hurricane balls come in many sizes, and large balls can be slightly larger than a standard basketball of 24.8 cm diameter (USA Basketball 2001). Large balls in the Gunter Library collection range from 34.5 cm diameter (from 1969 Hurricane Camille) to 53.5 cm diameter …

Sites 2c @ Und, University Of North Dakota

UND Publications

A 2009 brochure for a walking tour of buildings and landmarks on the University of North Dakota campus.

Prism #35, January 2009, Office Of The Provost

Prism: Western Michigan University's Newsletter for Academic Affairs

No abstract provided.

Rethinking Enablement In The Predictable Arts - Fully Scoping The New Rule, Bernard Chao

Sturm College of Law: Faculty Scholarship

In exchange for granting inventors a limited monopoly, the patent laws require inventors to "enable" the public to make and use their invention. In Liebel-Flarsheim Co. v. Medra4, Inc., Automotive Technologies International, Inc. v. BMW of North America, Inc., and Sitrick v. Dream works, L.L.C., the Federal Circuit made it far easier to show that patents are invalid based on lack of enablement in the predictable arts. These decisions rely on the enablement doctrine to invalidate claims that appear to be far broader in scope than what the written description of the patents suggests.

This Article: (1) explains the rationale …

Rethinking Regulations: Local Laboratories Inventing A Sustainable Idaho, Jerrold A. Long

Articles

No abstract provided.

Table Of Contents

Macalester Abroad: Research and Writing from Off-campus Study

No abstract provided.

2009 Nsu Sharks Track And Field, Nova Southeastern University

NSU Athletics Sports Programs and Media Guides

No abstract provided.

2009 Nsu Sharks Women's Tennis Media Guide, Nova Southeastern University

NSU Athletics Sports Programs and Media Guides

No abstract provided.

What Happens To Embryos When A Marriage Dissolves? Embryo Disposition And Divorce, Theresa M. Erickson, Megan T. Erickson

William Mitchell Law Review

No abstract provided.

The "Unnecessary" In-State Relocation Standard, Andrea Niemi, John Jerabek, Andrew Birkeland

William Mitchell Law Review

No abstract provided.

Southern Adventist University Graduate Catalog 2009-2010, Southern Adventist University

Catalog, Graduate

Southern Adventist University's graduate catalog for the academic year 2009-2010.

Southern Adventist University Undergraduate Catalog 2009-2010, Southern Adventist University

Catalog, Undergraduate

Southern Adventist University's undergraduate catalog for the academic year 2009-2010.