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Articles 91 - 120 of 132
Full-Text Articles in Entire DC Network
Binding Characteristics Study For Dengue Virus Non-Structural Protein 1 Of Antigen And Its Antibody By Using Circular Dichroism Technique, N. Ayusoff, N. Soin, N. Abd Rahman, R.N.Z. Raja Abd Rahman, M.K.B. Abd Kahar, F. Ibrahim
Binding Characteristics Study For Dengue Virus Non-Structural Protein 1 Of Antigen And Its Antibody By Using Circular Dichroism Technique, N. Ayusoff, N. Soin, N. Abd Rahman, R.N.Z. Raja Abd Rahman, M.K.B. Abd Kahar, F. Ibrahim
Research Publications (2006 to 2010)
This paper presents the binding characteristics study of dengue non-structural protein 1 (NS1) antigen and its antibody using circular dichroism technique in far UV region. Circular dichroism (CD) is a spectroscopic technique which measures differences in the absorption of left-handed and right handed circularly polarized light. The CD spectrum can determine conformation of the NS1 antigen and its antibody, conformational changes of the antigen-antibody interaction and estimates the secondary structure of these proteins in far UV region. Firstly, CD spectrum of individual solutions of the antigen and the antibody were measured. Then, the solutions were mixed to produce a solution …
National Cave And Karst Research Institute 2007-2008 Annual Report Nckri Annual Report, 2007-2008, National Cave And Karst Research Institute
National Cave And Karst Research Institute 2007-2008 Annual Report Nckri Annual Report, 2007-2008, National Cave And Karst Research Institute
KIP Articles
Contents: Executive Report -- NCKRI Projects -- Student Activities -- Outreach -- Organizational Structure -- Board Activities -- Board of Directors -- NCKRI Staff -- Staff Publications -- 2007-2008 Budget -- Strategic Plans.
Uri Undergraduate And Graduate Course Catalog 2009-2010, University Of Rhode Island
Uri Undergraduate And Graduate Course Catalog 2009-2010, University Of Rhode Island
URI Course Catalogs Archive
This is a digitized, downloadable version of the University of Rhode Island course catalog.
Arraycount, An Algorithm For Automatic Cell Counting In Microwell Arrays, Nezamoddin N. Kachouie, Kang Lifeng, Khademhosseini Ali
Arraycount, An Algorithm For Automatic Cell Counting In Microwell Arrays, Nezamoddin N. Kachouie, Kang Lifeng, Khademhosseini Ali
Mathematics and System Engineering Faculty Publications
Microscale technologies have emerged as a powerful tool for studying and manipulating biological systems and miniaturizing experiments. However, the lack of software complementing these techniques has made it difficult to apply them for many high-throughput experiments. This work establishes Arraycount, an approach to automatically count cells in microwell arrays. The procedure consists of fluorescent microscope imaging of cells that are seeded in microwells of a microarray system and then analyzing images via computer to recognize the array and count cells inside each microwell. To start counting, green and red fluorescent images (representing live and dead cells, respectively) are extracted from …
Louisiana Agriculture Winter, 2009
Enzymatic Quantification Of Phytate In Animal Manure, Zhongqi He, Heidi W. Waldrip, C. Wayne Honeycutt, M. Susan Erich, Zachary N. Senwo
Enzymatic Quantification Of Phytate In Animal Manure, Zhongqi He, Heidi W. Waldrip, C. Wayne Honeycutt, M. Susan Erich, Zachary N. Senwo
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Phytate (inositol hexaphosphate) has been identified as a major organic phosphorus (P) form in soil, animal manure, and other environmental samples. Although a number of methods are available for quantitative isolation and determination of phytate, they are time-consuming and not amenable to routine analysis. We developed a simple, rapid method for enzymatic determination of phytate in animal manure. Animal manure was extracted by H2O, 1M hydrochloric acid (HCl), 0.1M sodium acetate (NaOAc, pH5.0) with or without 0.05M ethylenediaminetetraacetate (EDTA), and 0.25M or 0.5M sodium hydroxide (NaOH)–0.05M EDTA. Extracts were diluted (1/10–1/150) and adjusted to pH5.0 in sodium acetate …
A Locus-Based Paradigm For Generating Systems Biological Inferences From Large Scale Functional Genomics Datasets, Ajish Dominic George
A Locus-Based Paradigm For Generating Systems Biological Inferences From Large Scale Functional Genomics Datasets, Ajish Dominic George
Legacy Theses & Dissertations (2009 - 2024)
Genomics data is growing at a exponential rate. The ability to integrate new results with existing knowledge about genomic biology is rapidly becoming the limiting factor as there no universal language with which to describe genomic functional elements. To integrate and compare new and existing genomic data, we define our basic functional unit of a genome to be a locus -- a set of positional coordinates along any genome with an arbitrary amount of functional annotations attached. The locus concept enables addressing genomic elements and annotations at any level of granularity from entire swaths of chromosomes to single base-positions. We …
Biotechnology For Immune Cell Detection And Evaluation : Devices Specifically Designed To Capture, Detect Or To Evaluate Alterations In Leukocyte Structure And Function, Thomas Joseph Zieziulewicz
Biotechnology For Immune Cell Detection And Evaluation : Devices Specifically Designed To Capture, Detect Or To Evaluate Alterations In Leukocyte Structure And Function, Thomas Joseph Zieziulewicz
Legacy Theses & Dissertations (2009 - 2024)
The advent of Nano-biotechnology has opened a whole new area of biological study that has allowed us to pattern, manipulate, separate, evaluate and detect biological structures and functions that were previously not possible. This dissertation will describe different types of micro-fabricated devices that were designed to quantify and/or evaluate the functional activities of cells of the immune system. The micro-fabricated devices were developed in collaboration with our colleagues at Princeton University as part of our Cellular Micro-dynamics Program of the Nanobiotechnology Center (NBTC) at Cornell University.
Passages, Volume 10, No. 2, Summer 2009, Joe Mitchell
Passages, Volume 10, No. 2, Summer 2009, Joe Mitchell
Texas Cave Management Association
Contents: TCMA: Cavers Protecting Caves -- View from the Palit -- Deep & Punkin Caves: Two Texas Classics -- TCMA -- Ezell's Cave: Home of the Blind Monster -- Godwin Ranch: Habitat for Endangered Species -- Rolling Oaks: A Small Preserve Protecting Small Creatures -- Robber Baron: History, Mystery, and Restoration -- Deep and Punkin Cave Survey Update -- LIDAR Mapping the Devil's Sinkhole -- Linda Palit: Highlights from a Lifetime of Caving -- LIDAR -- A Geology Field Trip to Deep & Punkin Caves -- RBC -- Whirlpool Preserve -- Lost Oasis Preserve -- TCMA Supports the 15th ICS …
Study Of Seasonal Influences On Algal Biodiversity In The River Yarqon (Central Israel) By Bio-Indication And Canonical Correspondence Analysis (Cca), Sophia Barinova, Moti Tavassi
Study Of Seasonal Influences On Algal Biodiversity In The River Yarqon (Central Israel) By Bio-Indication And Canonical Correspondence Analysis (Cca), Sophia Barinova, Moti Tavassi
Turkish Journal of Botany
In our research conducted in the River Yarqon during 2003-2006, we identified 313 taxa of algae and cyanobacteria belonging to 8 taxonomical divisions. Out of these 313 taxa, 268 taxa (85.6%) were indicators of environmental conditions that characterised the river water as alkaline with medium mineralisation. In the rainy and dry seasons the algal taxonomic compositions were very different, with prevailing diatoms in the winter and cyabobacteria and greens in summer. Bio-indication shows that the taxonomic preference for the self-purification process was more intensive during the rainy season, while the low level of river water in the dry season stressed …
Dna Sequence Representation By Use Of Statistical Finite Automata, Asmi Shah
Dna Sequence Representation By Use Of Statistical Finite Automata, Asmi Shah
Master's Projects
This project defines and intends to solve the problem of representing information carried by DNA sequences in terms of amino acids, through application of the theory of finite automata. Sequences can be compared against each other to find existing patterns, if any, which may include important genetic information. Comparison can state whether the DNA sequences belong to the same, related or entirely different species in the ‘Tree of Life’ (phylogeny). This is achieved by using extended and statistical finite automata. In order to solve this problem, the concepts of automata and their extension, i.e. Alergia algorithm have been used. In …
Design, Synthesis, And Characterization Of Novel Hydrophilic Fluorene-Based Derivatives For Bioimaging Applications, Dao Nguyen
Electronic Theses and Dissertations
In this work, hydrophilic fluorene-based derivatives that contain ethylene oxide substituents, have been synthesized and characterized for potential use as new fluorophores for bioimaging applications and for fluorescence sensing of heavy metals. Symmetrical and unsymmetrical fluorene derivatives based on structural types of acceptor-pi-acceptor, acceptor-pi-donor, and donor-pi-donor were characterized by TGA, UV-vis absorption, fluorescence emission, lifetime, anisotropy, and two-photon absorption (2PA) cross section. They were found to possess high thermal stability, high photostability, high fluorescence quantum yields, and generally large two-photon absorption cross sections, making them quite suitable for new probes in single-photon absorption and two-photon absorption fluorescence microscopy imaging. Novel …
Unionoida (Mollusca: Margaritiferidae, Unionidae) In Arkansas, Third Status Review, John L. Harris, William R. Posey Ii, C. L. Davidson, Jerry L. Farris, S. R. Oetker, J. N. Stoeckel, B. G. Crump, M. S. Barnett, H. C. Martin, J. H. Seagraves, M. W. Matthews, R. Winterringer, C. Osborne, Alan D. Christian, N. J. Wentz
Unionoida (Mollusca: Margaritiferidae, Unionidae) In Arkansas, Third Status Review, John L. Harris, William R. Posey Ii, C. L. Davidson, Jerry L. Farris, S. R. Oetker, J. N. Stoeckel, B. G. Crump, M. S. Barnett, H. C. Martin, J. H. Seagraves, M. W. Matthews, R. Winterringer, C. Osborne, Alan D. Christian, N. J. Wentz
Journal of the Arkansas Academy of Science
We analyzed stream inventories, phylogeographic studies, community and population estimates, life history and reproductive biology research, and suitable habitat investigations conducted from 1997-2008, as well as the Arkansas Natural Heritage Commission mussel database, to update the conservation status for all native freshwater unionoid bivalves thought to occur in Arkansas. Prior to this study, Harris et al. (1997) reviewed the distribution and status of 75 freshwater mussels considered native to Arkansas and ranked 22 species as endangered, threatened or special concern. We now recognize 85 mussel taxa in Arkansas; however, some of those have yet to be described or their nomenclature …
Optimization Strategies For Electrospun Silk Fibroin Tissue Engineering Scaffolds, Kristopher Kubow
Optimization Strategies For Electrospun Silk Fibroin Tissue Engineering Scaffolds, Kristopher Kubow
Selected Works
No abstract provided.
A New Look At Some Old Animals, Neil W. Blackstone
A New Look At Some Old Animals, Neil W. Blackstone
Faculty Articles, Papers, and Other Scholarship
none
Preparation Of The Chinese Sweet Leaf Tea Extract And Its Anti-Obesity Effect In Rodents, Gar Yee Koh
Preparation Of The Chinese Sweet Leaf Tea Extract And Its Anti-Obesity Effect In Rodents, Gar Yee Koh
LSU Master's Theses
The aqueous leaf extract of Rubus suavissimus (Chinese sweet leaf tea) was active in inhibiting angiogenesis, which could be used to suppress adipogenesis and cause weight loss. Following this revelation and approach, the anti-obesity effect was thoroughly investigated in normal and obese rat models. The investigations began with the preparation of quality-controlled extract. First, the crude extraction methods were examined leading to the characterization of the chemical composition. Second, the impure components were removed by alcohol precipitation and column chromatography, resulting in a standardized sweet leaf tea extract (RUS). Third, three bioavailable compounds identified from the urine analyses were re-constituted …
Synthesis Of Small Molecule Inhibitors Targeting Signal Transduction Pathways, Divya Ramamoorthy
Synthesis Of Small Molecule Inhibitors Targeting Signal Transduction Pathways, Divya Ramamoorthy
USF Tampa Graduate Theses and Dissertations
The main aim of the study described in this thesis is the development of small molecules as inhibitors targeting signal transduction pathways, thereby treating cancer. We attempted to synthesize compounds based on the hits obtained from high throughput screening of the Chemdiv diversity set compounds. Chapter One is a general introduction to cancer, history of chemotherapeutic drugs and an introduction to signal transduction pathways. The following two chapters briefly introduce the biological targets in the authors study. Chapter Two describes the role of B-cell lymphoma type xL (Bcl-xL), in apoptosis and the development of drugs targeting Bcl-xL. Examples of Bcl-xL …
The Physical And Genetic Framework Of The Maize B73 Genome, Fusheng Wei, Jianwei Zhang, Shiguo Zhou, Ruifeng He, Mary Schaeffer, Kristi Collura, David Kudrna, Ben P. Faga, Marina Wissotski, Wolfgang Gloser, Susan M. Rock, Tina A. Graves, Robert S. Fulton, Ed Coe, Patrick S. Schnable, David C. Schwartz, Doreen Ware, Sandra W. Clifton, Richard K. Wilson, Rod A. Wing
The Physical And Genetic Framework Of The Maize B73 Genome, Fusheng Wei, Jianwei Zhang, Shiguo Zhou, Ruifeng He, Mary Schaeffer, Kristi Collura, David Kudrna, Ben P. Faga, Marina Wissotski, Wolfgang Gloser, Susan M. Rock, Tina A. Graves, Robert S. Fulton, Ed Coe, Patrick S. Schnable, David C. Schwartz, Doreen Ware, Sandra W. Clifton, Richard K. Wilson, Rod A. Wing
Open Access Publications
No abstract provided.
Developmental Course Of Autistic Social Impairment In Males, John N. Constantino, Anna M. Abbacchi, Patricia D. Lavesser, Hannah Reed, Leah Givens, Lily Chang, Teddi Gray, Maggie Gross, Yi Zhang, Richard D. Todd
Developmental Course Of Autistic Social Impairment In Males, John N. Constantino, Anna M. Abbacchi, Patricia D. Lavesser, Hannah Reed, Leah Givens, Lily Chang, Teddi Gray, Maggie Gross, Yi Zhang, Richard D. Todd
Open Access Publications
No abstract provided.
Investigation And Quantification Of Codon Usage Bias Trends In Prokaryotes, Amanda L. Hanes
Investigation And Quantification Of Codon Usage Bias Trends In Prokaryotes, Amanda L. Hanes
Browse all Theses and Dissertations
Organisms construct proteins out of individual amino acids using instructions encoded in the nucleotide sequence of a DNA molecule. The genetic code associates combinations of three nucleotides, called codons, with every amino acid. Most amino acids are associated with multiple synonymous codons, but although they result in the same amino acid and thus have no effect on the final protein, synonymous codons are not present in equal amounts in the genomes of most organisms. This phenomenon is known as codon usage bias, and the literature has shown that all organisms display a unique pattern of codon usage. Research also suggests …
Productive Replication Of Malacosoma Neustria Nucleopolyhedrovirus (Manenpv) In Md203 Cell Line, İsmai̇l Demi̇r, Nurten Gürel, Remzi̇ye Nalçacioğlu, İkbal A. İnce, Zi̇hni̇ Demi̇rbağ
Productive Replication Of Malacosoma Neustria Nucleopolyhedrovirus (Manenpv) In Md203 Cell Line, İsmai̇l Demi̇r, Nurten Gürel, Remzi̇ye Nalçacioğlu, İkbal A. İnce, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
A plaque-purified genotypic variant, derived from a field isolate of the Malacosoma neustria nucleopolyhedrovirus (ManeNPV) from Turkey was characterized based on in vitro replication properties in a cell line, Md203 derived from Malacosoma disstria. The life cycle of ManeNPV was studied based on the cytopathic effects (CPEs), polyhedral inclusion body (PIB) formation, budded virus (BV) production, viral DNA replication and polyhedrin protein expression in ManeNPV-infected Md203 cells. Infection of Md203 cells with ManeNPV resulted in cytopathic effects within 24 and 36 h post infection and most cells detachted from the bottom of the culture plate on the fourth day. Typical …
Development And Application Of Dissociable Antibody Microarray (Dama) Staining Technique, Guanyuan Fu
Development And Application Of Dissociable Antibody Microarray (Dama) Staining Technique, Guanyuan Fu
LSU Doctoral Dissertations
Dissociable Antibody Microarray (DAMA) staining is a novel technique that integrates protein microarrays with conventional immunostaining techniques. It can simultaneously determine the expression and subcellular localizations (SCLs) of hundreds of proteins in cultured cells. I optimized this technology for protein expression and SCL profiling, and generated expression profile data analysis program DAMAPEP, molecular image database management program ChipView and automatic SCL assignment program DAMASCL. We demonstrated the application of this technique in the identification of potential biomarkers for breast cancer. We compared the expression profiles of 312 proteins among ten breast cell lines and identified 10 differentially expressed proteins. Among …
Optimization And Characterization Of Lab-On-A-Chip Elements: Microfluidic Chambers And Microneedles, Puneet Khanna
Optimization And Characterization Of Lab-On-A-Chip Elements: Microfluidic Chambers And Microneedles, Puneet Khanna
USF Tampa Graduate Theses and Dissertations
In this work, MEMS based fabrication is used to engineer multifaceted enhancements to microfluidic systems such as lab-on-a-chip devices. Two specific elements of microfluidic systems are the focus of this study: microfluidic chambers and microneedles. Microfluidic chambers, which are back-end passive elements, via proposed material and structural modifications, are shown to exhibit reduced non-specific DNA binding and enable increased cell lysis efficiency. Microneedles, which are front-end interfacing elements, have been fabricated in silicon and in silicon dioxide varieties. The geometry of silicon microneedles has been varied via DRIE processing to yield sharpened tips. Sharpening of microneedle tips provides reduced skin …
Noncovalent Interaction Of Platinum Planar Amine Compounds With Tryptophan: A Strategy To Interfere With P53-Mdm2 Interactions And Targeting Retroviral Zn Finger-Dna Interaction (Hiv Ncp7), Aaron Bate
Theses and Dissertations
Non-covalent interactions involving π-π stacking play an essential role in self-assembly and molecular recognition processes such as protein folding and DNA/RNA-protein selective recognition. The knowledge gained from these studies could provide insight into possible site recognition complexes, inhibiting or mimicking protein-protein or protein-DNA interactions. Based on molecular modeling as well as HOMO and LUMO energies, several chromophores were selected with a variety of ∆ε values (∆ε= |εHOMO,NAcTrp – εLUMO,chromophores|), high and low, to establish a correlating trend with the modeling and experimental data. The corresponding Pt(dien) compounds were synthesized and their ability to stack to N-acetyl tryptophan was evaluated by …
Breaking Barriers, Pushing Promise: American's Need For An Embryonic Stem Cell Regulatory Scheme, Sylvia E. Simson
Breaking Barriers, Pushing Promise: American's Need For An Embryonic Stem Cell Regulatory Scheme, Sylvia E. Simson
Brooklyn Journal of International Law
No abstract provided.
First Approaches To Standard Protocols And Reference Materials For The Assessment Of Potential Hazards Associated With Nanomaterialss, Gordon Chambers, Hugh Byrne, Iseult Lynch, Micheal Riediker, Hans Bouwmeester, Hans Marvin, Alan Casey, Markus Berges, Martin Clift, Teresa Fernandez, Lise Fjellsbo, Lucienne Juillerat, Gert Roebben, Christoph, Klein, Qinglan Wu, Vince Hackley, Jean-Pierre Kaiser, Wolfganag Kreyling, Michael Garner, Peter Hatto,, Kenneth Dawson
First Approaches To Standard Protocols And Reference Materials For The Assessment Of Potential Hazards Associated With Nanomaterialss, Gordon Chambers, Hugh Byrne, Iseult Lynch, Micheal Riediker, Hans Bouwmeester, Hans Marvin, Alan Casey, Markus Berges, Martin Clift, Teresa Fernandez, Lise Fjellsbo, Lucienne Juillerat, Gert Roebben, Christoph, Klein, Qinglan Wu, Vince Hackley, Jean-Pierre Kaiser, Wolfganag Kreyling, Michael Garner, Peter Hatto,, Kenneth Dawson
Articles
All new technologies have an inherent risk, which is typically assessed alongside the development of applications of the technology. This is also the case for nanotechnology: a key concern in the case of engineered nanomaterials (ENMs) is that due to their very small size, NMs can reach areas such as the cell that are inaccessible to other materials, such as implants and drugs. As a result of their large surface area, NMs may be more reactive than other larger materials. The large physical and chemical variability of NMs, and the fact that small changes can have large consequences, mean that …
Comparative Susceptibilities Of Six Insect Cell Lines To Infection By Malacosoma Neustria Nucleopolyhedrovirus (Manenpv), İsmai̇l Demi̇r, Nurten Gürel, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Comparative Susceptibilities Of Six Insect Cell Lines To Infection By Malacosoma Neustria Nucleopolyhedrovirus (Manenpv), İsmai̇l Demi̇r, Nurten Gürel, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
In this study, 6 lepidopteran cell lines derived from Trichoplusia ni (Tn5), Lymantria dispar (LdElta), Bombyx mori (Bm-5), Spodoptera frugiperda (Sf21), Spodoptera exigua (Se-1), and Choristoneura fumiferana (Cf-124T) were investigated for susceptibility to Malacosoma neustria nucleopolyhedrovirus (ManeNPV) isolated in Turkey. Infection of Malacosoma disstria (Md203) cell line by ManeNPV was used as a control. In ManeNPV-Md203 infection, polyhedral inclusion bodies (PIBs) were detected at 36 h p.i. and observed in all infected cells by 120 h p.i., and the infectious virus titer increased 10^3-fold. At 24 h p.i., the efficient replication of viral DNA was detected. In this study, we …
Bottom Boundary Layer Physics And Sediment Transport Along A Transgressive Sand Body, Ship Shoal, South-Central Louisiana: Implications For Fluvial Sediments And Winter Storms, Daijiro Kobashi
LSU Doctoral Dissertations
Ship Shoal, a shore-parallel sand body, was recently recognized as having a unique physical and biological environment and also as a potential sand resource for coastal restoration in coastal Louisiana. Little is known regarding such dynamics, in concert with fluvial sediments and winter storms, influenced in unique ecosystems, and likely in future potential sand mining. This dissertation addresses such the morphodynamics and sedimentary processes and their implications for the mining from the shoal using field measurements and numerical modeling studies. During the winter-spring season, fluvial sediment plumes shifted from the prevailing west to southeast during the post-frontal phases, resulted in …
The Communicating Dance, Alison Plevey
The Communicating Dance, Alison Plevey
Theses : Honours
Does dance have the ability to communicate universally to all observers at some level of human engagement? This paper discusses theatre dance as a human communicative medium, the characteristics of how it communicates and to a certain degree why and what it communicates. In doing so the intentions of the communicating dancer and choreographer are considered alongside that of the receptive and responsive audience. The thesis compiles a survey of textual sources pin pointing the unique communicative abilities of dance, as an artistic, expressive and meaningful form of non-verbal communication establishing suggestions of the ways it may be universally communicable.
Microbial Diversity And Geochemical Energy Sources Of Tutum Bay, Ambitle Island, Papua New Guinea, An Arsenic-Rich, Shallow-Sea Hydrothermal System, Nancy Akerman
All Theses and Dissertations (ETDs)
I investigate the hydrothermal system located in Tutum Bay, Ambitle Island, Papua New Guinea, a shallow-sea system ~5 - 10 meters below sea level that is arsenic-rich. Hydrothermal vents in the bay expel fluids with arsenite: AsIII) concentrations as high as 950 μg/L. To determine the role that Tutum Bay microorganisms might play in mediating As-redox reactions, three approaches were used: analyzing the geochemical environment for energy sources, characterizing the archaeal community composition of the sediments, and conducting culture-dependent As-cycling experiments. The second chapter of this dissertation discusses an energetic study of potential chemolithotrophic metabolic reactions, including As-redox reactions. Results …