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Sample Prep For Proteomics Of Breast Cancer: Proteomics And Gene Ontology Reveal Dramatic Differences In Protein Solubilization Preferences Of Radioimmunoprecipitation Assay And Urea Lysis Buffers, Lambert Cm Ngoka

Chemistry Publications

Background

An important step in the proteomics of solid tumors, including breast cancer, consists of efficiently extracting most of proteins in the tumor specimen. For this purpose, Radio-Immunoprecipitation Assay (RIPA) buffer is widely employed. RIPA buffer's rapid and highly efficient cell lysis and good solubilization of a wide range of proteins is further augmented by its compatibility with protease and phosphatase inhibitors, ability to minimize non-specific protein binding leading to a lower background in immunoprecipitation, and its suitability for protein quantitation.

Results

In this work, the insoluble matter left after RIPA buffer extraction of proteins from breast tumors are subjected …

Town Report Milford, New Hampshire 2007., Milford Town Representatives

Milford, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

Course Catalog 2008-2009, Laguardia Community College

Course Catalogs

No abstract provided.

Faculty Publications And Creative Works 2008, Office Of The Vice President For Research

Office of the Vice President for Research

Faculty Publications & Creative Works is an annual compendium of scholarly and creative activities of University of New Mexico faculty during the noted calendar year. It serves to illustrate the robust and active intellectual pursuits conducted by the faculty in support of teaching and research at UNM.

Quantum: Research, Scholarship & Creative Works At The University Of New Mexico, 2008, Office Of The Vice President For Research

Office of the Vice President for Research

No abstract provided.

Schedule, Spring 2008, Taos Registrar

Office of the Taos Registrar - Archive

Schedule of courses for the Taos campus for the spring semester of 2008.

Hurricane Impacts On Bottlenose Dolphins In The Northern Gulf Of Mexico, Patricia E. Rosel, Heidi Watts

Gulf of Mexico Science

No abstract provided.

Analysis Of Secchi Depths And Light Attenuation Coefficients In The Louisiana-Texas Shelf, Northern Gulf Of Mexico, A. Lugo-Fernández, M. Gravois, T. Montgomery

Gulf of Mexico Science

We analyzed Secchi depths (Zs), scalar downwelling irradiance, and transmissometer data collected during 10 cruises that spanned two and one-half years (May 1992-Nov. 1994) over the Louisiana-Texas shelf. The Zs increases from the inner shelf (depths ≤50 m) to the outer shelf and from nonsummer (Zs =10m) to summer (Zs = 14m). These changes in Zs are induced by the shelf's circulation and freshwater input, both of which vary seasonally, i.e., nonsummer to summer. Optical parameters (e.g., Zs), like the circulation, divide the shelf into an inner and outer shelf along the 50-m isobath. The …

Dietary Analysis Of Batfishes (Lophiiformes: Ogcocephalidae) In The Gulf Of Mexico, Bronson H. Nagareda, Jonathan M. Shenker

Gulf of Mexico Science

Stomach content analyses, performed on three species of batfishes, Halieutichthys aculeatus, Ogcocephalus declivirostris, and Ogcocephalus pantosticus collected in the Gulf of Mexico in summer (June-July) and fall (Oct.-Nov.) 2002 and 2003, revealed a variety of benthic invertebrates, particularly gastropods, polychaete worms, and xanthid crabs. Schoener's dietary overlap indices (Sl) were calculated between the three species within the same seasons, and within each species between seasons. SI values indicated that each species consumed a different assemblage of prey and that two of the species exhibited temporal variation in diet.

Relative Abundance And Distribution Of Sand Seatrout (Cynoscion Arenarius) In Relation To Environmental Conditions, Habitat, And River Discharge In Two Florida Estuaries, Anthony R. Knapp, Caleb H. Purtlebaugh

Gulf of Mexico Science

The sand seatrout, Cynoscion arenarius (Ginsburg, 1930), is an abundant recreational and commercial species that resides primarily in the nearshore and estuarine waters of the Gulf of Mexico. We examined relative abundance and distribution of sand seatrout [individuals >100 mm standard length (SL)] in relation to environmental conditions and river discharge in the Tampa Bay (1997-2004) and Charlotte Harbor (1999-2004) estuaries on the west coast of Florida. Fish were collected during a long-term fisheries-independent monitoring program with a 183-m purse seine. Sand seatrout were most abundant over deep, muddy substrates devoid of seagrass. Smaller sand seatrout between145 mm SL and …

Effects Of Porous Mesh Groynes On Macroinvertebrates Of A Sandy Beach, Santa Rosa Island, Florida, U.S.A., W.J. Keller, C.M. Pomory

Gulf of Mexico Science

The use of porous mesh groynes to accrete sand and stop erosion is a relatively new method of beach nourishment. Five groyne, five intergroyne, and five control transects outside the groyne area on a beach near Destin, FL were sampled during the initial 3 mo after installment of groynes for Arenicola cristata (polychaete) burrow numbers, benthic macroinvertebrate numbers, and dry mass. Salinity, temperature, turbidity, and current velocity were measured at one location within the groyne site and control site. Current velocity was reduced and sand was accreted in the groyne site relative to the control site. Few significant changes or …

In Memoriam: L. Scott Quackenbush (1951-2008)

Gulf of Mexico Science

No abstract provided.

Patterns Of Distribution Along A Salinity Gradient In The Flatback Mud Crab Eurypanopeus Depressus, Julie Van Horn, S. Gregory Tolley

Gulf of Mexico Science

To measure the effect of salinity on estuarine communities, spatial and temporal variations in population distribution were investigated in the flatback mud crab Eurypanopeus depressus (Smith, 1869), a dominant species on oyster reefs in southwest Florida. Eurypanopeus depressus were collected using lift nets (1 m² ) deployed at three morphologically homologous stations along a salinity gradient in each of three estuaries. They were sampled monthly during three seasonally wet and three seasonally dry months. An analysis of abundance showed that although this species can survive extreme salinities, it is most common in moderate environments. Biomass is also highest at …

Life History Parameters Of Gulf Flounder (Paralichthys Albigutta) From Northwest Florida, Gary R. Fitzhugh, William A. Fable Jr., Wallace L. Trent

Gulf of Mexico Science

Age, growth, natural and total mortality, a length-weight relationship, reproductive seasonality, sex ratio, maturity, and reproductive potential by size were estimated for gulf flounder, Paralichthys albigutta, from northwest Florida. The study used a fishery-independent approach during three annual migratory spawning seasons to sample gulf flounder offshore (spearing by divers) and inshore (multipanel trammel nets). We found gulf flounder to be slightly smaller at L∞ than other paralichthids (using either von Bertalanffy or damped growth functions), but gulf flounder obtained larger sizes and older ages than previously thought for this species [females to 575 mm total length (TL) and age …

Genetic Structure Of Halodule Wrightii Populations From The Laguna Madre Region In The Western Gulf Of Mexico, Patrick D. Larkin, Krista L. Heideman, Joyce E. Parker, Beau Hardegree

Gulf of Mexico Science

A random amplified polymorphic DNA assay was used to assess genetic variation in populations of the seagrass Halodule wrightii (Ascherson) from the western Gulf of Mexico. This region includes one of the world's few hypersaline lagoons (Laguna Madre) and contains the vast majority of seagrasses on the Texas coast. Results indicate a moderate amount of genetic diversity among populations. The highest level (H_c= 0.33) was found in a population from Nueces Bay, a disturbed site in the Coastal Bend area, whereas the lowest was found in a Lower Laguna Madre population (H_c = 0.15). Genetic differentiation generally …

Small-Scale Recruitment Of Flora To A Newly Developed Tidal Inlet In The Northwest Gulf Of Mexico, Ryan L. Fikes, Roy L. Lehman

Gulf of Mexico Science

No abstract provided.

Bioinformatics Workshop, Center For Science And Mathematics Education

Teacher Resource Documents

No abstract provided.

Sickle Cell Workshop, Center For Science And Mathematics Education

Teacher Resource Documents

No abstract provided.

Usm Summer Math Institute: Ti84 Basics, Center For Science And Mathematics Education

Teacher Resource Documents

No abstract provided.

Usm Summer Math Institute: Area Of A Triangle And Geometric Series; Wind And Surge Spreadsheet And Bar Graph, Center For Science And Mathematics Education

Teacher Resource Documents

No abstract provided.

Usm Summer Math Institute: Geometry And Equilateral Triangles, Center For Science And Mathematics Education

Teacher Resource Documents

No abstract provided.

The Gulf Coast's Barrier Islands, Center For Science And Mathematics Education

Teacher Resource Documents

No abstract provided.

Biological Sciences Learning Center Fourth Annual Haunted Halloween Trail, Biological Sciences Learning Center

Teacher Resource Documents

No abstract provided.

Engineering Research 2008, College Of Engineering, Michigan Technological University

Michigan Tech Engineering Magazine

Table of Contents

• Energy
• Air & Water
• Engineered Materials
• Sensing & Imaging
• Research Centers & Institutes
• People
• Innovation & Technology Transfer

Improving Post-Approval Risk Surveillance For Drugs: Active Post-Market Risk Identification, Matthew Gordon

Michigan Telecommunications & Technology Law Review

Pre-approval clinical trials cannot possibly ensure that a drug will not have disastrous side effects once it arrives on the market. Post-approval drug safety data gathering was put in place to address this problem, but as implemented, it has not proven to be as effective as hoped. Congress recently overhauled the legislation regarding post-approval drug risk identification, and in doing so made a deliberate decision to put much of the burden of post-approval drug surveillance on the FDA through data mining. Further, the legislation gave the FDA the power to require post-approval clinical trials from drug makers only in limited …

The Clinical Efficacy Of Reflexology In Nursing Home Residents With Dementia, Nancy A. Hodgson

Selected Works of Nancy Hodgson, PhD

No abstract provided.

The Leaning Object: April 2009, John C. Owens

Leading Object

Perspectives, Vegetable gardening becomes a focus on ‘Backyard Farmer’, Economics of water topic of conference, Managing Tough Times Web site launched,Erwin transfers ag education into banking career, IANR molecular life sciences report being finalized, Three IANR faculty receive major systemwide awards, Three receive OEA recognition, Booker, Wegulo recipients of Dinsdale awards, Henneman receives ACE award

The Leading Object: November/December 2008, John C. Owens

Leading Object

Perspectives; National Science Foundation grant advances4-H robotics curriculum; Extension dean named to CSREES Hall of Fame; UNL genetics course spurs Hoegemeyer’s interest in plant breeding; Holly completes PGA education; Faculty members visit Zamorano University in Honduras; Retirement opens new chapter for Mayo; Hamernik, Jackson named associate ARD deans; Osborne presents leadership awards

Introducing A New Technology To Enhance Community Sustainability: An Investigation Of The Possibilities Of Sun Spots, S Tantisureeporn, L J. Armstrong

EDU-COM International Conference

The introduction of the Sun SPOT, Small Programmable Object Technology, developed by Sun Microsystems has been depicted as providing a revolutionary change in cyber physical interaction. Based on Sun Java Micro Edition (ME), this sensor technology has the potential to be used across a number of discipline areas to interface with systems, the environment and biological domains. This paper will outline the potential of Sun SPOTs to enhance community sustainability. An action based research project was carried out to investigate the potential uses of these technologies and develop a prototype system as a proof of concept. The research will compare …

A Double Call To Service: Elizabeth Beiring Kim ’94 Helps Protect The Oceans Through Her Work With Epa, Ub Law Forum

UB Law Forum

No abstract provided.