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2008

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Cytokinesis Defects In Budding Yeast, Katherine Stockstill Apr 2008

Cytokinesis Defects In Budding Yeast, Katherine Stockstill

Undergraduate Research Conference at Missouri S&T

Budding yeast are a very good research organism, because they are similar to humans in many ways. One of the research opportunities with budding yeast is that there are many genes that are uncharacterized, and it is not known what their cellular function is or what their functional role is. One way to study gene function is to knock out the gene. We focused on uncharacterized genes that have been identified in genomic screens as interacting with cytokinesis proteins. Another way to study gene function is to tag genes on the chromosomes. The purpose of tagging genes is to allow …

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Biological Breathalyzer, Brian Pink, Jacqueline Schneinder Apr 2008

Biological Breathalyzer, Brian Pink, Jacqueline Schneinder

Undergraduate Research Conference at Missouri S&T

The aim of this research is the construction of a biological breathalyzer through synthetic biology. The metabolic pathways of a species of the Pichia taxa are used. The yeast is able to metabolize both ethanol and methanol. The first known by-product of methanol metabolism is the AO enzyme from the AOX gene. When both ethanol and methanol are present, the yeast prefers to metabolize ethanol, so the AOX gene is not expressed. The AOX gene promoter is fused with a fluorescence protein gene so expression of the AOX gene can be visually detected. When the cell is supplied with both …

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In Vitro Assessment Of Porous Glass Scaffolds For Bone Tissue Engineering, Kevin Walker Apr 2008

In Vitro Assessment Of Porous Glass Scaffolds For Bone Tissue Engineering, Kevin Walker

Undergraduate Research Conference at Missouri S&T

Two types of glass scaffolds fabricated from silicate-based 13-93 glass were tested for possible use in bone tissue engineering. The two scaffold types included constructs prepared by a polymer foam infiltration procedure to simulate trabecular bone and sintered glass fiber construct. These scaffolds were seeded with mouse MLO-AS cells, an established line of osteogenic cells used as a model of developing bone tissue. Assays were conducted to assess growth and function of the seeded cells over incubation times ranging from two to six days. Cell adhesion to the scaffolds was confirmed by scanning electron microscopy. Quantitative measurements of total cellular …

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Energy Efficient Walking Mechanism With Genetic Algorithm Synthesis, Robert Adams Apr 2008

Energy Efficient Walking Mechanism With Genetic Algorithm Synthesis, Robert Adams

Undergraduate Research Conference at Missouri S&T

This research covers the use of legs for transportation rather than wheels. Legged walking is potentially more efficient than wheels on deformable surfaces such as sand, where the required transportation energy is related to the amount of sand displaced. Available robotic walking devices use multiple motors per leg to control movement, which allows multiple degrees of freedom, but consumes excessive energy. Because the center of mass remains at a constant elevation, this new mechanism consumes no energy except for joint and sliding friction of the legs while traveling on level ground. Previously, no available mechanisms were efficient enough for a …

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Field Analyses For The Detection And Enumeration Of Coliform Bacteria In Drinking Water During A Public Health Assessment Study, Jenna Tune Apr 2008

Field Analyses For The Detection And Enumeration Of Coliform Bacteria In Drinking Water During A Public Health Assessment Study, Jenna Tune

Undergraduate Research Conference at Missouri S&T

The microbiological contaminant concentrations of drinking water are typically analyzed using a customary membrane filtration method approved by the U.S. Environmental Protection Agency (EPA) and the World Health Organization (WHO), as well as the European Economic Community (EEC). The membrane filtration method is well documented providing highly reproducible water quality data at a relatively high cost in terms of preparation time, in situ procedure, and money. The commercially available Coliscan EasyGel method for the detection and enumeration of Escherichia coliand total coliforms costs much less. However, the usability of the field data may be uncertain when the results are qualitatively …

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Synthesis Of A Biological Breathalyzer, Herman C. Armstrong, Rachel Klapper, Brian Pink, Morgan Schiermeier, Jackie Schneider Apr 2008

Synthesis Of A Biological Breathalyzer, Herman C. Armstrong, Rachel Klapper, Brian Pink, Morgan Schiermeier, Jackie Schneider

Undergraduate Research Conference at Missouri S&T

The aim of this research is the construction of a biological breathalyzer through the culturing of yeast cells that can be used to measure concentrations of ethanol. The project makes use of the metabolic pathways of species of the Pichia taxa that are able to metabolize both ethanol and methanol. The yeast prefers to metabolize ethanol when both methanol and ethanol are present and will therefore consume ethanol before methanol. The AO enzyme, from the AOX gene, appears to be the first enzyme produced by the metabolism of methanol. The AOX gene will not be expressed in the cell when …

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Construction And Testing Of A Biological Breathalyzer, Rachel Klapper, Herman Armstrong, Brian Pink, Morgan Schiermeier, Jackie Schneider Apr 2008

Construction And Testing Of A Biological Breathalyzer, Rachel Klapper, Herman Armstrong, Brian Pink, Morgan Schiermeier, Jackie Schneider

Undergraduate Research Conference at Missouri S&T

The ultimate goal of this research was the construction of a biological breathalyzer using methods of synthetic biology. The metabolic pathways of Pichia taxa were utilized for this research. This yeast is able to metabolize both ethanol and methanol present in the environment. The first known by-product of methanol metabolism is the AO enzyme from the AOX gene. When both ethanol and methanol are present, the yeast preferentially metabolizes ethanol and the AOX gene is not expressed. By fusing the AOX gene promoter with a fluorescence protein gene, the expression of the AOX gene may be visually detected. When the …

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Biological Breathalyzer, Morgan Schiermeier, Herman Armstrong, Rachel Klapper, Brian Pink, Jackie Schneider Apr 2008

Biological Breathalyzer, Morgan Schiermeier, Herman Armstrong, Rachel Klapper, Brian Pink, Jackie Schneider

Undergraduate Research Conference at Missouri S&T

The aim of this research is the construction of a biological breathalyzer through synthetic biology, specifically through use of the metabolic pathways of a species of the Pichia taxa. The yeast utilized is able to metabolize both ethanol and methanol. However, when both ethanol and methanol are present, the yeast prefers to metabolize ethanol such that an AOX gene is not expressed because the first known by-product of methanol metabolism is the AO enzyme from the AOX gene. The AOX gene promoter is fused with a fluorescence protein gene so expression of the AOX gene can be visually detected. When …

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Design And Testing Of A Biological Breathalyzer, Jacqueline Schneider, Herman Armstrong, Rachel Klapper, Brian Pink, Morgan Schiermeier Apr 2008

Design And Testing Of A Biological Breathalyzer, Jacqueline Schneider, Herman Armstrong, Rachel Klapper, Brian Pink, Morgan Schiermeier

Undergraduate Research Conference at Missouri S&T

The aim of this research is the construction of a biological breathalyzer through synthetic biology. The metabolic pathways of a species of the Pichia taxa are used. The yeast is able to metabolize both ethanol and methanol. The first known by-product of methanol metabolism is the AO enzyme from the AOX gene. When both ethanol and methanol are present, the yeast prefers to metabolize ethanol, so the AOX gene is not expressed. The AOX gene promoter is fused with a fluorescence protein gene so expression of the AOX gene can be visually detected. When the cell is supplied with both …

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Identifying Ribosomal Protein Gene Families In Glycine Max, Daniel Schwent Apr 2008

Identifying Ribosomal Protein Gene Families In Glycine Max, Daniel Schwent

Undergraduate Research Conference at Missouri S&T

The database at NCBI is a vast resource of genetic information. The purpose of this project was to search through, acquire, compile and test that data in a way that would identify putative gene families of the proteins that make up the cytoplasmic ribosomes in Glycine max. The initial search criteria were sequences that had been submitted to NCBI as portions of these ribosomal protein genes in Glycine and were later expanded to include sequences from some of Glycine's close relatives. The search results were assembled into contiguous sequences, searched for open reading frames and finally compared to each other …

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4th Annual Undergraduate Research Conference Abstract Book, Missouri University Of Science And Technology Apr 2008

4th Annual Undergraduate Research Conference Abstract Book, Missouri University Of Science And Technology

Undergraduate Research Conference at Missouri S&T

No abstract provided.

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The Register, 2008-04-09, North Carolina Agricutural And Technical State University Apr 2008

The Register, 2008-04-09, North Carolina Agricutural And Technical State University

NCAT Student Newspapers

No abstract provided.

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Student Scholarship Day 2008, Grand Valley State University Apr 2008

Student Scholarship Day 2008, Grand Valley State University

Student Scholars Day Archives

No abstract provided.

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Spartan Daily, April 9, 2008, San Jose State University, School Of Journalism And Mass Communications Apr 2008

Spartan Daily, April 9, 2008, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily (School of Journalism and Mass Communications)

Volume 130, Issue 38

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The Antelope, University Of Nebraska At Kearney Apr 2008

The Antelope, University Of Nebraska At Kearney

The Antelope

No abstract provided.

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4th Annual Undergraduate Research Conference Winners, Missouri University Of Science And Technology Apr 2008

4th Annual Undergraduate Research Conference Winners, Missouri University Of Science And Technology

Undergraduate Research Conference at Missouri S&T

No abstract provided.

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Serum Proteomic Profiles In Inflammatory And Non-Inflammatory Cardiomyopathies: A Novel Approach For Diagnostic Biomarker Discovery, Oyere Onuma Apr 2008

Serum Proteomic Profiles In Inflammatory And Non-Inflammatory Cardiomyopathies: A Novel Approach For Diagnostic Biomarker Discovery, Oyere Onuma

Yale Medicine Thesis Digital Library

The aim of this project is to develop a noninvasive serum test that predicts histologic forms of myocarditis (inflammatory) and dilated (non-inflammatory) cardiomyopathy using proteomic techniques to analyze serum proteins. Idiopathic dilated cardiomyopathy (DCM) and myocarditis (myocardial inflammation) represent a spectrum of heart muscle disease of various etiologies that usually present with progressive heart failure. Together, they constitute the leading cause of heart transplantation in the United States. Currently, the gold standard of diagnosis of myocarditis is by endomyocardial biopsy (EMB) and histopathological classification according to the Dallas Criteria ; however this diagnostic technique is severely limited by its invasiveness, …

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Platelet-Derived Growth Factor Enables Direct Derivation Of Oligodendrocyte Progenitors From Cns Stem Cells, Rajesh Rao Apr 2008

Platelet-Derived Growth Factor Enables Direct Derivation Of Oligodendrocyte Progenitors From Cns Stem Cells, Rajesh Rao

Yale Medicine Thesis Digital Library

Oligodendrocytes derived in the laboratory from stem cells have been proposed as a treatment for acute and chronic injury to the central nervous system (CNS). Platelet-derived growth factor-receptor alpha (PDGFRα) signaling is known to play an important role for regulation of oligodendrocyte progenitor cell numbers both during development and adulthood. Here, we analyze the effect of PDGFRα signaling on CNS stem cells derived from embryonic day 13.5 murine cortex and cultured in monolayer. Fetal and adult CNS stem cells express PDGFRα, and PDGF-AA treatment increases viability and proliferation of these cells. In the absence of insulin, this effect of PDGF-AA …

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Banner News, Jessi Smith, Molly Lumley, Fiona Flatt, Shane Meier, Eden Hinrichs Apr 2008

Banner News, Jessi Smith, Molly Lumley, Fiona Flatt, Shane Meier, Eden Hinrichs

Banner News

No abstract provided.

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Volume 45, Number 33: April 09, 2008, University Of North Dakota Apr 2008

Volume 45, Number 33: April 09, 2008, University Of North Dakota

University Letter Archive

No abstract provided.

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The Spinnaker Vol. 31 No. 30, University Of North Florida Apr 2008

The Spinnaker Vol. 31 No. 30, University Of North Florida

Spinnaker

Student newspaper for the UNF community

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The Bg News April 9, 2008, Bowling Green State University Apr 2008

The Bg News April 9, 2008, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. April 9, 2008. Volume 98-Issue 136.

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Epi-Genetic Abnormalities Of Infantile Hemangiomas: Regulation Of The Igf2/H19 Locus, Brent Schultz Apr 2008

Epi-Genetic Abnormalities Of Infantile Hemangiomas: Regulation Of The Igf2/H19 Locus, Brent Schultz

Yale Medicine Thesis Digital Library

Purpose: To investigate the epi-genetic regulation of Insulin Like Growth Factor 2 (IGF2) and its reciprocally imprinted transcript H19 in infantile hemangiomas. Introduction: Infantile hemangiomas (IH) are the most common childhood tumor of the head and neck. Despite their prevalence and potentially morbid sequelae, little is known regarding the pathogenesis of this disease. However, a tumorigenic molecule, Insulin Like Growth Factor 2 (IGF2), has been implicated by microarray and confirmatory Real-Time PCR studies. There is substantial documentation that methylation abnormalities within the IGF2 and neighboring H19 loci are related to the overproduction of IGF2 in many distinct tumor types. An …

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The Immunoepidemiology Of Hookworm Infection In The Peruvian Amazon, Priya Shete Apr 2008

The Immunoepidemiology Of Hookworm Infection In The Peruvian Amazon, Priya Shete

Yale Medicine Thesis Digital Library

Hookworm infection caused by Ancylostoma duodenale or Necator americanus is a significant global health threat, causing chronic anemia, malnutrition, developmental delay, enteritis, and increased susceptibility to non-parasitic diseases. One of the most prevalent of the neglected tropical diseases, hookworm infection affects almost 1 billion people worldwide, particularly in developing countries. Current methods for diagnosis and treatment of hookworm infection are largely the same as they have been for the past century. However, several recent advances in the molecular characterization of hookworm virulence factors now provide researchers with an improved understanding of disease pathogenesis, potential targets for treatment and novel antigens …

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The Guardian, April 9, 2008, Wright State University Student Body Apr 2008

The Guardian, April 9, 2008, Wright State University Student Body

The Guardian Student Newspaper

Twenty page issue of The Guardian, the official student-run newspaper for Wright State University. The Guardian has been published regularly since March of 1965.

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Unlv Authors Honored For Publications In 2007, Unlv Lied Library Apr 2008

Unlv Authors Honored For Publications In 2007, Unlv Lied Library

Faculty Author Receptions

Lists UNLV authors and their publications who were honored at the 2008 UNLV authors reception. Includes cover art.

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Spartan Daily, April 8, 2008, San Jose State University, School Of Journalism And Mass Communications Apr 2008

Spartan Daily, April 8, 2008, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily (School of Journalism and Mass Communications)

Volume 130, Issue 37

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Unh Marks Earth Day All Week Long April 18 - 27, Beth Potier Apr 2008

Unh Marks Earth Day All Week Long April 18 - 27, Beth Potier

Media Relations

No abstract provided.

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The Daily Gamecock, Tuesday, April 8, 2008, University Of South Carolina, Office Of Student Media Apr 2008

The Daily Gamecock, Tuesday, April 8, 2008, University Of South Carolina, Office Of Student Media

April

No abstract provided.

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Montana Kaimin, April 8, 2008, Students Of The University Of Montana, Missoula Apr 2008

Montana Kaimin, April 8, 2008, Students Of The University Of Montana, Missoula

Montana Kaimin, 1898-present

Student newspaper of the University of Montana, Missoula.

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