Digital Commons Network^™

Conservation Of Botanicals Used For Dental And Oral Healthcare In Ekiti State, Nigeria, Joshua Kayode, Michael Ayorinde Omotoyinbo

Ethnobotanical Leaflets

A combination of social surveys and direct field observations was used to identify and determine the abundance status of chewing stick botanicals in the tree zones of Ekiti State, Nigeria. Voucher specimens of the botanicals identified by the respondents were collected, identified and relevant information on them were documented. The relative abundance of the identified species was determined based on their accessibility when required. Thus species were classified into two groups, abundant species and scarce species. A total of 49 species belonging to 26 families were identified as being used for dental and oral health care in the study area …

Nanotechnology - Scientific And Regulatory Challenges, David B. Fischer

Villanova Environmental Law Journal (1991 - )

No abstract provided.

Gold & Blue, Winter 2008, St. Mary's University- San Antonio, Texas

Gold & Blue

This is the Winter 2008 edition of Gold & Blue which is St. Mary's University's official school publication.

Do Reasonable Efforts Require Cultural Competence - The Importance Of Culturally Competent Reunification Services In The California Child Welfare System, Nell Clement

UC Law Journal of Race and Economic Justice

Despite the disproportionate number of culturally diverse families represented in the California child welfare system, there exists no statutory requirement that families in crisis be provided with reunification services that are culturally competent. This note examines the evolution of the child welfare system in United States in recent decades and the current child welfare law in California. This note discusses the negative effect of removal of culturally diverse children from their families and communities and the specific aspects of the child welfare system that play a part in this removal. Clement concludes by advocating for statutory recognition of culture in …

The State Of Coral Reef Ecosystems Of Southeast Florida, Chantal Collier, Rob Ruzicka, Ken Banks, Luiz Barbieri, Jeff Beal, David Bingham, James Bohnsack, Sandra Brooke, Nancy Craig, Richard E. Dodge, Lou Fisher, Nick Gadbois, David S. Gilliam, Lisa Gregg, Todd Kellison, Vladimir Kosmynin, Brian Lapointe, Erin Mcdevitt, Janet Phipps, Nikki Poulos, John Proni, Patrick Quinn, Bernhard Riegl, Richard E. Spieler, Joanna Walczak, Brian K. Walker, Denise Warrick

Marine & Environmental Sciences Faculty Reports

The northern extension of the Florida reef tract and a complex of limestone ridges run parallel to the subtropical Atlantic coastline of southeast Florida. Spanning 170 km from the northern border of Biscayne National Park (BNP) in Miami-Dade County to the St. Lucie Inlet in Martin County, the reefs and hardbottom areas in this region support a rich and diverse biological community (Figure 5.1). Nearshore reef habitats in southeast Florida include hardbottom areas, patch reefs and worm reefs (Phragmatopoma spp.) exhibiting abundant octocoral, macroalgae, stony coral and sponge assemblages. Offshore, coral reef associated biotic assemblages occur on linear Holocene Acropora …

Point / Counterpoint: What It Means To Be A "College Of The Church", Robert Benne, Thomas Christenson

Intersections

No abstract provided.

Medicine@Yale March/April 2008 Volume 4, Issue 1, Yale School Of Medicine Office Of Communications

Medicine@Yale - development newsletter

Medicine@Yale : advancing biomedical science, education and health care was a print and online newsletter which published news about academics, research and philanthropy for alumni, donors, faculty, students and the general public. It was initially published 6 times a year and then changed to a quarterly schedule. The first issue came out June/Jul. 2005. The final issue was published in May/June 2022, vol. 17, issue 1. Published by Yale School of Medicine, Office of Communications.

Medicine@Yale September/October 2008 Volume 4, Issue 4, Yale School Of Medicine Office Of Communications

Medicine@Yale - development newsletter

Medicine@Yale : advancing biomedical science, education and health care was a print and online newsletter which published news about academics, research and philanthropy for alumni, donors, faculty, students and the general public. It was initially published 6 times a year and then changed to a quarterly schedule. The first issue came out June/Jul. 2005. The final issue was published in May/June 2022, vol. 17, issue 1. Published by Yale School of Medicine, Office of Communications.

Medicine@Yale July/August 2008 Volume 4, Issue 3, Yale School Of Medicine Office Of Communications

Medicine@Yale - development newsletter

Medicine@Yale : advancing biomedical science, education and health care was a print and online newsletter which published news about academics, research and philanthropy for alumni, donors, faculty, students and the general public. It was initially published 6 times a year and then changed to a quarterly schedule. The first issue came out June/Jul. 2005. The final issue was published in May/June 2022, vol. 17, issue 1. Published by Yale School of Medicine, Office of Communications.

Inferences, Arguments, And Second Generation Forensic Evidence, Erin E. Murphy

Faculty Articles

Advances in scientific and technological evidence have resulted in a new, second generation of forensic proof characterized by its high degree of probativeness, mechanical sophistication, and database-based investigative capacity. Methods such as DNA typing are already in widespread use in the criminal justice system, and law enforcement may soon have commonly available other novel techniques such as advanced biometrics, electronic locators, or even reliable lie detection systems. The promise and allure of such forms of forensic evidence rest in its high degree of accuracy. But the perception that such evidence is effectively infallible has resulted in an increasing number of …

The Truth About The Unseen Bugs Around The Farm, At The Fair, And In Your Food!, Cooperative Extension Service

SDSU Extension Special Series

The following is a lesson plan to teach children in grades K-8 about foodborne illness, particularly E. coli O157:H7. Several activities are included – you may choose or adapt those that are appropriate for age group and education level. This educational tool includes the following:
1. Ideas for introduction of subject.
2. Information needed to lecture about and explain E. coli O157:H7 and other pathogens.
3. Activities for children to develop a better understanding of the subject.
4. Additional resources.
We hope that this will be a valuable tool for educating children and decreasing their risk of becoming infected with …

The New Racial Preferences, Devon W. Carbado, Cheryl I. Harris

Faculty Articles

Michigan's Proposal 2 and California's Proposition 209 explicitly prohibit their state governments from discriminating or granting "preferential treatment . . . on the basis of race." Proponents of both ballot initiatives specifically employed this language to eliminate state promulgated race-based affirmative action programs. For advocates of Proposal 2 and Proposition 209, affirmative action is the quintessential example of a preference on the basis on race. They reasoned that the policy benefits blacks and Latinos and burdens whites and, in some formulations, Asian Americans. This Article neither defends affirmative action nor critiques anti-affirmative action initiatives. Instead, we take Proposition 209 and …

Why We Do The Things We Do? The Role Of Ethics In Water Resource Planning, Amy Hardberger

Faculty Articles

Water provides a natural framework in the role of ethics because ethical issues are present in every facet of water management. The value of water and the creation of ethics dictate decisions regarding water resource management. Value can be assessed from factors including happiness, well-being, or intrinsic value. Once a value is assessed, obligations that dictate actions regarding this issue are generated, and an ethic is created.

Various domestic and international policies have, both explicitly and implicitly, called for a human right to water. The presence of domestic and international policies that recognize or protect a person’s right to water …

Development Of Fragment-Specific Osteopontin Antibodies And Elisa For Quantification In Human Metastatic Breast Cancer, Alicia Plumer, Hongyi Duan, Sripriya Subramaniam, F. Lee Lucas, Susan Miesfeldt, Ah-Kau Ng, Lucy Liaw

Applied Medical Sciences [Discontinued]

Background: Osteopontin (OPN) is associated with human cancers, and circulating blood OPN may have diagnostic or prognostic value in clinical oncology. Methods: To evaluate OPN as a cancer biomarker, we generated and characterized five novel mouse monoclonal antibodies against the human full-length OPN (fl-OPN). Epitopes recognized by four antibodies (2C5, 2F10, 2H9, and 2E11) map to N-terminal OPN (aa1-166); one (1F11) maps to C-terminal OPN (aa167-314). These antibodies recognize recombinant and native OPN by ELISA and immunoblot, cross reacting with human and mouse OPN. Two of these novel antibodies ( 2F10 and 1F11) were used to develop a quantitative enzyme …

The Voice, Winter 2008: Volume 53, Issue 2, Dordt College

The Voice Archive

Promoting Politics: Iowa Caucuses Prompt Action on Campus; Return on Investment; Campolo Challenges Comfortable Christians; Korean Exchange Grows; Dordt and Dutch University Offer International Exchange Program; Students Watch Pigs Do the Work; One-Act Festival Makes for Another Grand Weekend; Schelhaas Honored With Highest Regional Theater Award; Collaborative Ag Ed Program Could Ease Teacher Shortage; New Employees; Film Challenge Is Just That; Another Way to Do Business; Career Service is Available; Dordt Expands Nursing Enrollment; Dordt Prairie Is Becoming a Reality; KSP: Kuyper Scholars Thrive on Academic Challenge; Wolthuis Pens Book on Leadership Training; Faculty News; Fall Teams Make Way for …

Engineered Nanoscale Materials And Derivative Products: Regulatory Challenges, Linda-Jo Schierow

Congressional Research Service Reports

Scientists and engineers can now examine, design, and manipulate materials at the molecular level, termed “nanoscale,” between 1 and 100 billionths of a meter. The U.S. government has invested heavily to ensure that American industry remains a global leader in the field, because the products of nanotechnology are seen to have great economic potential and offer possible solutions to national problems ranging from energy efficiency to detection of agents of biological warfare. Optimism about nanotechnology is tempered, however, by concerns about the unknown potential of nanoscale materials to harm the environment and human health. Some have called for federal regulation …

Wind Scour Of Navajo Sandstone At The Wave (Central Colorado Plateau, U.S.A.), David B. Loope, Winston M. Seiler, Joseph A. Mason, Marjorie A. Chan

Department of Earth and Atmospheric Sciences: Faculty Publications

At the Wave, a photogenic landform on the Utah–Arizona border, modern, southwesterly, sand-carrying winds abrade the Navajo Sandstone. Abundant trains of centimeter-scale, transverse, upwind-facing treads and risers cut sedimentary structures at a high angle. Central to the formation of these erosional steps are crusts produced by microbes lying just beneath exposed sandstone surfaces. Treads and risers are present on the walls of smoothly curved troughs at the Wave, on the walls of nearby circular scour pits, and on bedrock domes found at the center of scour pits. Because of their locations and orientations, the large-scale troughs and scour pits could …

A General And Rapid Cell-Free Approach For The Interrogation Of Protein–Protein, Protein–Dna, And Protein–Rna Interactions And Their Antagonists Utilizing Split-Protein Reporters, Jason R. Porter, Cliff I. Stains, Benjamin W. Jester, Indraneel Ghosh

Cliff Stains Publications

Split-protein reporters have emerged as a powerful methodology for imaging biomolecular interactions which are of much interest as targets for chemical intervention. Herein we describe a systematic evaluation of split-proteins, specifically the green fluorescent protein, β-lactamase, and several luciferases, for their ability to function as reporters in completely cell-free systems to allow for the extremely rapid and sensitive determination of a wide range of biomolecular interactions without the requirement for laborious transfection, cell culture, or protein purification (12–48 h). We demonstrate that the cell-free split-luciferase system in particular is amenable for directly interrogating protein–protein, protein–DNA, and protein–RNA interactions in homogeneous …

Conserved Sequences Of Sperm-Activating Peptide And Its Receptor Throughout Evolution, Despite Speciation In The Sea Star Asterias Amurensis And Closely Related Species, Mia Nakachi, Motonori Hoshi, Midori Matsumoto, Hideaki Moriyama

Hideaki Moriyama Publications

The asteroidal sperm-activating peptides (asterosaps) from the egg jelly bind to their sperm receptor, a membrane-bound guanylate cyclase, on the tail to activate sperm in sea stars. Asterosaps are produced as single peptides and then cleaved into shorter peptides. Sperm activation is followed by the acrosome reaction, which is subfamily specific. In order to investigate the molecular details of the asterosap-receptor interaction, corresponding cDNAs have been cloned, sequenced and analysed from the Asteriinae subfamily including Asterias amurensis, A. rubens, A. jorbesi and Aphelasterias japonica, as well as Distolasterias nipon from the Coscinasteriinae subfamily. Averages of 29% and 86% identity …

Computer Simulation Of Radio Frequency Heating Of Model Fruit Immersed In Water, Sohan Birla, Shaojin Wang, Juming Tang

Department of Engineering Mechanics: Faculty Publications

The present study investigated the effect of different factors on temperature distribution within a spherical object in a parallel plate radio frequency (RF) heating system. A finite element-computer simulation program - FEMLAB was used to solve the electromagnetic and Navier-Stoke equations for developing a model to study the effect of dielectric properties of a model fruit and its surrounding medium. The model fruit was prepared from 1% gellan gel for experimental validation of the simulation results. The results showed that spherically-shaped samples surrounded with air between RF electrodes and placed in the proximity of electrodes would not heat uniformly. Immersing …

Cutting Edge: Langerin/Cd207 Receptor On Dendritic Cells Mediates Efficient Antigen Presentation On Mhc I And Ii Products In Vivo, Juliana Idoyaga, Cheolho Cheong, Koji Suda

Publications

The targeted delivery of Ags to dendritic cell (DCs) in vivo greatly improves the efficiency of Ag presentation to T cells and allows an analysis of receptor function. To evaluate the function of Langerin/CD207, a receptor expressed by subsets of DCs that frequently coexpress the DEC205/CD205 receptor, we genetically introduced OVA into the C terminus of anti-receptor Ab H chains. Taking advantage of the new L31 mAb to the extracellular domain of mouse Langerin, we find that the hybrid Ab targets appropriate DC subsets in draining lymph nodes and spleen. OVA is then presented efficiently to CD8⁺ and CD4 …

Cd80+Cd205+ Splenic Dendritic Cells Are Specialized To Induce Foxp3+ Regulatory T Cells, Sayuri Yamazaki, Diana Dudziak, Gordon Heidkamp

Publications

Foxp3⁺CD25⁺CD4⁺ regulatory T cells (Treg) mediate immunological self-tolerance and suppress immune responses. A subset of dendritic cells (DCs) in the intestine is specialized to induce Treg in a TGF-β- and retinoic acid-dependent manner to allow for oral tolerance. In this study we compare two major DC subsets from mouse spleen. We find that CD8⁺ DEC-205/CD205⁺ DCs, but not the major fraction of CD8^- DC inhibitory receptor-2 (DCIR2)⁺ DCs, induce functional Foxp3⁺ Treg from Foxp3^- precursors in the presence of low doses of Ag but without added TGF-β. CD8 ^{+ …}

Dendritic Cells In Vivo: A Key Target For A New Vaccine Science, Ralph M. Steinman

Publications

Dendritic cells (DCs) are the antigen presenting cells that initiate and regulate immunity. By studying these cells in vivo, we will be able to move beyond standard approaches and design vaccines that directly harness the elaborate properties of DCs to control immunity.

Conditional Protein Alleles And Regulation Of Bacterial Transcription: A Thesis In Two Acts, Edmund Ching Schwartz

Student Theses and Dissertations

The use of small molecule modulators of protein function (drugs) has gained in popularity due to the speed and precision with which they can work. Unfortunately, a small molecule with the required specificity and potency is not always available. We have developed two methods to generate small molecule sensitive protein alleles without the need to screen for a new drug. In the first, which we term conditional protein splicing (CPS), an intein is split into two inactive fragments which are activated by heterodimerization. By fragmenting the target protein and fusing the pieces to this split intein, we were able to …

A Structural And Biochemical Analysis Of The Drosophila Protein Period, Heather Anne King

Student Theses and Dissertations

Circadian clocks regulate changes in behavior and physiology that occur with a period of approximately 24 hours and are based on negative feedback loops. The molecular components of circadian clocks are conserved among animals, and a key element in all such clocks is the protein Period (PER), a circadian transcription inhibitor. The stable production, posttranslational modification, and nuclear translocation of PER all contribute to the timing of the clock. This work describes the synthesis and purification of various Drosophila PER fragments for biochemical and crystallographic analysis. Several stable PER fragments are identified, including one crystallizable fragment. The structure of the …

Proteomic, Bioinformatic And Functional Characterization Of The Nuclear Pore Complex Of The African Trypanosome, Jeffrey Allen Degrasse

Student Theses and Dissertations

The eukaryotic genome, and its associated proteins, is intricately packaged and sequestered within the boundary of a double membrane, known as the nuclear envelope (NE). Transport across the NE is mediated by large protein assemblages known as nuclear pore complexes (NPCs). Yeast and vertebrate NPCs are comprised of about 30 proteins, termed nucleoporins (Nups), which are present in multiple copies. The origins and evolution of the nucleus and NPC are not yet clear, although it seems likely that the nucleus arose only once in eukaryotic evolution. To further our understanding of the evolution of the NPC, we characterized the NPC …

Cracking The Odor Code: Molecular And Cellular Deconstruction Of The Olfactory Circuit Of Drosophila Larvae, Kenta Asahina

Student Theses and Dissertations

The Drosophila larva offers a powerful model system to investigate the general principles by which the olfactory system processes behaviorally relevant sensory stimuli. The numerically reduced larval olfactory system relieves the formidable molecular and cellular complexity found in other organisms. This thesis presents a study in four parts that investigates molecular and neuronal mechanisms of larval odor coding. First, the larval odorant receptor (OR) repertoire was characterized. ORs define the olfactory receptive range of an animal. Each of the 21 larval olfactory sensory neurons (OSNs) expresses one or rarely two ORs, along with the highly conserved olfactory co-receptor Or83b. Second, …

Small Molecules As Probes For Cell Division And Intracellular Transport, Ulf Peters

Student Theses and Dissertations

Cell-permeable small molecules that can act on their targets on fast time scales are powerful probes of cell division mechanisms and intracellular transport processes. Phenotype-based screens with chemical libraries have been used to identify such inhibitors. However, probes for most proteins are still not available, and the requirements on compound collections to yield such probes are not well understood. Here I present two approaches to find and use such probes. First, in an attempt to find probes for cell division, I have shown that a small collection of 100 diaminopyrimidines yielded a range of cell division phenotypes, including changes in …

Discovery Of The First Endogenous Gain Of Function Mutation In Drosophila Ras1 As A Dominant Suppressor Of Apoptosis, Christopher Gafuik

Student Theses and Dissertations

The development of multicellular organisms requires a tight coordination of cell proliferation, cell differentiation and cell death in order to correctly specify cell fate and number. According to the trophic theory of survival, this is achieved in part by a competition between cells in a tissue for a limited number of extracellular survival factors. Cells that do not receive sufficient quantities of these survival cues engage a default cell death program and are thereby eliminated. This 'social control' of cell survival ensures the integrity of tissues by matching the correct number of different cell types to each other. Apoptosis is …

Further Insight Into The Two Functions Of Telomeres: Determining The Role Of Tankyrase1 In Telomere Length Regulation And Tin2 In Telomere Protection, Jill Donigian

Student Theses and Dissertations

Tankyrase1 is a multifunctional poly(ADP-ribose) polymerase that can localize to telomeres through its interaction with the shelterin component TRF1. Tankyrase1 poly(ADP-ribosyl)ates TRF1 in vitro, and its nuclear overexpression leads to loss of TRF1 and telomere elongation, suggesting that tankyrase1 is a positive regulator of telomere length. In agreement with this proposal, we showed that tankyrase1 RNA interference results in telomere shortening proportional to the level of knockdown, while a tankyrase1-resistant form of TRF1 enforced normal telomere length control. Thus, in human cells, tankyrase1 appears to act upstream of TRF1, promoting telomere elongation through the removal of TRF1. This pathway appears …