Digital Commons Network^™

Isolation And Cloning Of Human Papillomavirus 16 L1 Gene From Iranian Isolate, Ali Teimoori, Hoorieh Soleimanjahi, Fatemeh Fotouhi, Zahra Meshkat

Saudi Medical Journal

OBJECTIVE: To isolate and construct cloning and expression vectors containing human papillomavirus (HPV16-L1) gene as target for application as recombinant vaccine. METHODS: The study was performed in 2007 in Tarbiat Modares University, Tehran, Iran. Four genital specimens DNAs were amplified with the use of HPV type-specific primers based on HPV-16 L1, E6, and E7 genes. The polymerase chain reaction products were cloned into suitable cloning and expression vectors and were confirmed by restriction enzyme analysis and sequencing. RESULTS: The desired plasmids were sequenced and indicated 99% homology with those submitted full length L1 sequences in the GenBank. CONCLUSION: The results …

Estimates For Worldwide Laboratory Animal Use In 2005, Katy Taylor, Nicky Gordon, Gill Langley, Wendy Higgins

Laboratory Experiments Collection

Animal experimentation continues to generate public and political concern worldwide. Relatively few countries collate and publish animal use statistics, yet this is a first and essential step toward public accountability and an informed debate, as well as being important for effective policy-making and regulation. The implementation of the Three Rs (replacement, reduction and refinement of animal experiments) should be expected to result in a decline in animal use, but without regular, accurate statistics, this cannot be monitored. Recent estimates of worldwide annual laboratory animal use are imprecise and unsubstantiated, ranging from 28–100 million. We collated data for 37 countries that …

Multiple-Criteria Decision Making For Integrated Watershed Management In The Ramian Watershed, Golestan, Iran, Amir Sadoddin, Vahedberdi Sheikh, Raoof Mostafazade, Mohammad Ghassem Halili

International Congress on Environmental Modelling and Software

This research has concentrated on the physical and socio-economic impacts of vegetation-based management scenarios targeting on flooding and soil erosion issues in the Ramian sub-watersheds. The Ramian watershed with an approximate area of 24000 ha is located in Golestan, Iran. For each sub-watershed, four biological actions (activities) and 16 management scenarios have been considered. Physical impacts have been studied using the SCS (Soil Conservation Service) hydrologic model and the EPM (Erosion Potential Method) model. Economic and social impacts have been evaluated using the cost/benefit analysis as well as the examination of the results of a social survey. Some physical, social, …

Ethnobotany, Phytochemistry And Pharmacology Of Mussaenda Species (Rubiaceae), K. S. Vidyalakshmi, Hannah R. Vasanthi, G. V. Rajamanickam

Ethnobotanical Leaflets

The genus Mussaenda is an important source of medicinal natural products, particularly iridoids, triterpenes and flavonoids. The purpose of this paper is to cover the more recent developments in the ethnobotany, pharmacology and phytochemistry of this genus. The species in which the largest number of compounds has been identified is Mussaenda pubescens. Pharmacological studies have also been made, however, of other species in this genus. These lesser known plants of the genus are described here according to their cytotoxicity, anti-inflammatory, antiviral, antioxidant and antibacterial properties. The information given here is intended to serve as a reference tool for practitioners …

Proteomics Analysis Identifies Molecular Targets Related To Diabetes Mellitus-Associated Bladder Dysfunction, Elizabeth Yohannes, Jinsook Chang, Mark R. Chance

Faculty Scholarship

Protein expression profiles in rat bladder smooth muscle were compared between animal models of streptozotocin-induced diabetes mellitus (STZ-DM) and age-matched controls at 1 week and 2 months after induction of hyper-glycemia with STZ treatment. At each time point, protein samples from four STZ-DM and four age-matched control rat bladder tissues were prepared independently and analyzed together across multiple DIGE gels using a pooled internal standard sample to quantify expression changes with statistical confidence. A total of 100 spots were determined to be significantly changing among the four experimental groups. A subsequent mass spectrometry analysis of the 100 spots identified a …

Endometrioid Adenocarcinoma 13 Years After Total Abdominal Hysterectomy And Bilateral Salpingo-Oophorectomy., Ayman Al-Talib, Lucy Gilbert, Jocelyne Arseneau

Saudi Medical Journal

ABSTRACT Malignant transformation is an infrequent complication of endometriosis. As endometriosis is an ectopic endometrium, hyperestrogenism may cause hyperplasia or transformation into cancer. We describe a case of a 68-year-old woman who underwent total abdominal hysterectomy and bilateral salpingo-oophorectomy for endometriosis. She was subsequently placed on estrogen-only replacement therapy. She presented with left-sided pelvic mass and shortness of breath. Computed tomography of chest, pelvis, and abdomen, demonstrated right-sided pleural effusion and soft tissue mass in the pelvis. Pleural effusion was tapped and biopsy from the peritoneal mass showed metastatic adenocarcinoma; immunohistochemistry findings favored endometrioid adenocarcinoma. She was treated by 6 …

Biology, Saint Mary's College Of California

Academic Catalog

No abstract provided.

Research News: 2008, No. 2, University Of Mississippi. School Of Pharmacy

Research News: Grants and Publications (2000-2014)

Grants, publications, technology transfer, special announcements

The Beginning Of The End For Chimpanzee Experiments?, Andrew Knight

Experimentation Collection

The advanced sensory, psychological and social abilities of chimpanzees confer upon them a profound ability to suffer when born into unnatural captive environments, or captured from the wild – as many older research chimpanzees once were – and when subsequently subjected to confinement, social disruption, and involuntary participation in potentially harmful biomedical research. Justifications for such research depend primarily on the important contributions advocates claim it has made toward medical advancements. However, a recent large-scale systematic review indicates that invasive chimpanzee experiments rarely provide benefits in excess of their profound animal welfare, bioethical and financial costs. The approval of large …

Contract And Grant Awards Fiscal Year 2008, Office Of The Vice President For Research

Office of the Vice President for Research

I invite you to read this report Contract & Grant Awards, fiscal year 2008, which lists contract and grant UC&GV awards received by the University of New Mexico (UNM) during the period from July 1, 2007 - June 30, 2008 (FY08). These awards represent new funds that were acquired during FY08 by the main campus, branch campuses, education centers, and the Health Sciences Center (HSC). The HSC includes the School of Medicine, the College of Nursing, and the College of Pharmacy. The awards received for FY08 total $303.4M, of which $169.3M is attributed to the main campus and $134.1M to …

Separation And Characterization Of Respirable Amphibole Fibers From Libby, Montana, James S. Webber, David J. Blake, Tony Ward, Jean C. Pfau

Public and Community Health Sciences Faculty Publications

The vermiculite mine in Libby, Montana, was in operation for over 70 yr and was contaminated with asbestos-like amphibole fibers. The mining, processing, and shipping of this vermiculite led to significant fiber inhalation exposure throughout the community, and residents of Libby have developed numerous pulmonary diseases such as lung cancer and mesothelioma. The present study describes the separation of Libby 6-mix into respirable and nonrespirable size fractions by means of aqueous elutriation. The elutriator, designed to separate fibers with aerodynamic diameters smaller than 2.5 μm (respirable) from larger fibers, used an upward flow rate of 3.4 × 10^{−4 …}

Geographic Variation In Killer Whale Attacks On Humpback Whales In The North Pacific: Implications For Predation Pressure, Gretchen H. Steiger, John Calambokidis, Janice M. Straley, Louis M. Herman, Salvatore Cerchio, Dan R. Salden, Jorge Urban-R., Jeff K. Jacobsen, Olga Von Ziegesar, Kenneth C. Balcomb, Christine M. Gabriele, Marilyn E. Dahlheim, Senzo Uchida, John K. B. Ford, Paloma Ladron De Guevara-P., Manami Yamaguchi, Jay Barlow

United States Department of Commerce: Staff Publications

We examined the incidence of rake mark scars from killer whales Orcinus orca on the flukes of humpback whales Megaptera novaeangliae throughout the North Pacific to assess geo- graphic variation in predation pressure. We used 3650 identification photographs from 16 wintering or feeding areas collected during 1990 to 1993 to determine conservative estimates in the percentage of whales with rake mark scarring. Dramatic differences were seen in the incidence of rake marks among regions, with highest rates on wintering grounds off Mexico (26 vs. 14 % at others) and feeding areas off California (20 vs. 6% at others), 2 areas …

Commencement Program 2008, Loma Linda University

Commencement Programs

CONTENTS

2 | 2008 Events of Commencement

3 | The Academic Procession

4 | Significance of Academic Regalia

5 | The Good Samaritan

6 | The Speakers

14 | The University Honorees

24 | The School Honorees

35 | The Programs

36 | School of Medicine

47 | School of Pharmacy

52 | School of Dentistry

62 | School of Science and Technology and School of Religion

70 | School of Nursing

76 | School of Allied Health Professions - Physical Therapy

82 | School of Allied Health Professions

91 | School of Public Health

Microbial Colonization Of Deep-Sea Hydrothermal Vent Chimneys, Antoine Pagé

Dissertations and Theses

Deep-sea hydrothermal vent chimneys harbor some of the most extreme environments supporting life on Earth. In the absence of sunlight, the microbial communities that colonize these deposits are based on energy harvested by chemolithoautotrophs exploiting the chemical disequilibria created as hydrothermal fluids mix with seawater. Although controversial, several models consider deep-sea vent chimneys as likely habitats for primitive ecosystems that developed during the Archean.

The goal of this study was to describe spatial and temporal patterns of microbial colonization in deep-sea vent chimneys emitting high temperature (>300°C) fluids. Microbial community composition and structure were evaluated through analyses of 16S …

Observer, Central Washington University

Student Newspaper

Student newspaper for Central Washington University for May 8, 2008

Rest Maintains Self-Renewal And Pluripotency Of Embryonic Stem Cells, Sanjay K Singh, Mohamedi N Kagalwala, Jan Parker-Thornburg, Henry Adams, Sadhan Majumder

Faculty, Staff and Student Publications

The neuronal repressor REST (RE1-silencing transcription factor; also called NRSF) is expressed at high levels in mouse embryonic stem (ES) cells, but its role in these cells is unclear. Here we show that REST maintains self-renewal and pluripotency in mouse ES cells through suppression of the microRNA miR-21. We found that, as with known self-renewal markers, the level of REST expression is much higher in self-renewing mouse ES cells than in differentiating mouse ES (embryoid body, EB) cells. Heterozygous deletion of Rest (Rest+/-) and its short-interfering-RNA-mediated knockdown in mouse ES cells cause a loss of self-renewal-even when these cells are …

Snp Characterizaiton And Genetic And Molecular Analysis Of Mutants Affecting Fiber Development In Cotton, Chuanfu An

Theses and Dissertations

Cotton (Gossypium spp.) is the world’s leading textile fiber crop, and an important source of oil and protein. Insufficient candidate gene derived-markers suitable for genetic mapping and limited information on genes that control economically important traits are the major impediments to the genetic improvement of Upland cotton (G. hirsutum L.). The objectives of this study were to develop a SNP marker discovery strategy in tetraploid cotton species, SNP characterization and marker development from fiber initiation and elongation related genes, chromosomal assignment of these genes by SNP marker-based deletion analysis or linkage mapping, and genetic and molecular analysis of mutants affecting …

Analysis Of The Cyc1 Promoter In Candida Albicans, Tomica Blocker

McCabe Thesis Collection

Scientists are considering two factors that may be important in the pathogenesis of C. albicans', it's capability to morph from yeast to hyphal phase, and its differences in colony morphology, cell shape, cell surface and cell permeability. (5) Unfortunately, unlike the bacteria, yeasts do not yet have proven virulence factors. However, science is suggesting that some of the major factors which contribute to the virulence of Candida are its ability to form hyphae, its ability to resist phagocytosis, its ability to adhere to epithelial cell surfaces, its ability to grow well at 37 degrees Celsius, and its ability to secrete …

Synthetic Studies And Biological Evaluation Of Imidazole-Containing Marine Alkaloids, Lesley Ann Schmid

Chemistry & Biochemistry Dissertations - Archive

This dissertation discusses the synthetic methods towards and the preliminary biological studies of an unnamed, imidazole-containing alkaloid, derived from the marine species Oceanapia. This alkaloid was first discovered in 1996 by researchers at the NIH as a possible inhibitor of the key enzyme in the detoxification cycle of Mycobacterium tuberculosis. Chapter 1 provides an overview of tuberculosis, which is currently the second highest cause of death worldwide, with one third of the world's population infected. In recent years, the emergence of multi-drug resistant strains has created new interest and funding for tuberculosis research. New treatments like the alkaloid described above …

Carbon, Nitrogen, Sodium And Phosphorous Concentrations And Ratios In Spartina Alterniflora (Loiser) From Sites Of Various Freshwater And Nutrient Inputs In Coastal Louisiana Wetlands, Margaret Frances Williamson

Honors Capstones

No abstract provided.

A Behavioral Assay To Quantify Retinal Degeneration In X. Laevis, Jacob Fleming

Renée Crown University Honors Thesis Projects - All

Currently, 3.5 million Americans over the age of 40 are blind or visually impaired. Cases of visual impairment can be attributed to a number of causes. Retinitis pigmentosa, glaucoma, and macular degeneration all involve some form of damage to the retina. While medical advances continue to aid those with vision loss, there is currently no cure for visual impairment due to cell death. In the age of stem cells however, there is hope for those with retinal damage.

Seven eye field transcription factors play specific roles in eye development. When injected into developing Xenopus laevis, these transcription factors induce …

Influential Parasites: Importance Of Haemosporidian Parasite Infection In The White-Throated Sparrow, Amanda P. Jamison

All-Inclusive List of Electronic Theses and Dissertations

The study of parasite-mediated selection, as originally postulated by Hamilton and Zuk ( 1982) in birds is an area of intense study as it allows us to test the significance of the relationship between host and parasite. Recent developments of resistance to treatments in humans for parasite induced disease, creates a place for this important research and emphasizes the need to find the connections and interactions in this relationship between ecology and infectious disease. The development of new molecular techniques has made the study of parasitism in birds and mammals easier and provides an efficient sensitive . methodology to detect …

Comparison Of Fruit Removal In Tropical Tree Species Between Forest And Urban Areas – Monteverde, Costa Rica, May 2008, Dominic Derenge

Monteverde Institute: Tropical Ecology and Conservation

Tropical frugivorous birds are facing the effects of increased human land development. The conversion of forests to road and buildings interrupts the interaction forest trees and their frugivorous dispersers. This study uses the tree/frugivore mutualism to asses the effect proximity to human influences has upon a forest health. Measurements of the removal rates of Citharexylum costaricensis (Verbenaceae) fruits in a fragment near to the forest displayed significantly higher removal rates than in the urban fragment (Paired T-Test, T=0.996 df=15, p = 0.043, n =16). Separate trials with Solanum rugosum (Solanaceae) fruit displayed the same trend (Paired T-Tests, T = 0.695, …

Tendencies And Perspectives Of Social Sciences In Health In Latin America, Everardo D. Nunes

English

No abstract provided.

Healing, Learning And Play, Kangana Bhatnagar

Theses and Dissertations

Research suggests that the first five years of life are critical for building the foundation for children’s success throughout their schooling and life. Throughout these first years of life there are a number of essential windows of opportunity during which certain kinds of stimuli are needed to help the brain develop and maintain critical connections necessary for learning. This project is a unification of a Child Development Center and a therapeutic center. This center includes children who suffer from bereavement; a broken home, death of a parent or child abuse. These children are provided with therapy by a specialist, and …

A Literature Review: Current Trends In Holistic Nursing, Angela J. Wolf

Senior Honors Theses

There is scarcity of documentation that seeks to define spirituality relating to holistic nursing; consequently, a literature review was formulated to define spirituality and guide nursing practice towards recognizing the importance of and implementation of spiritual care. By researching the current trends in peer-reviewed journals from the past three years and analyzing associated articles, this paper addresses the need for a comprehensive definition of spirituality. Key concepts such as belief, values, interconnectedness with self, others and God, energy, hope and transcendence will be analyzed, and the accumulated data will be complied into a framework that is easy for a nurse …

Technos Prize Awarded To Student Heading To Africa, Rachel Hatch

News and Events (Discontinued Series)

No abstract provided.

Fifth Annual Undergraduate Research & Scholarship Conference 2008, Boise State University

Past Undergraduate Research and Scholarship Conferences

No abstract provided.

Kahn Chronicle: Spring 2008, Smith College, Kahn Liberal Arts Institute

Kahn Chronicle

Table of Contents:

The Kahn Institute "On the Road"
Announcements
Spotlight on Students
Interim Reports: Sustainable Houses, Homes and Communities; Undergrounds Underworlds
Spring 2008 Short-Term Projects
Updates on New Project Forms
Spotlight on Faculty
A Word From the Director

Systems Biology: The Solution To Understanding Alcohol-Induced Disorders?, National Institute On Alcohol Abuse And Alcoholism

United States Public Health Resources

With the decoding of the human genome, researchers gained access to extensive information about the molecules in the body, how they function in the healthy organism, and how they may contribute to certain diseases. However, despite this wealth of information, many questions remain about the ways in which these molecules interact to control more complex phenomena, including the development of many common disorders. Systems biology offers scientists a promising tool with which to deal with such complexities and may revolutionize the prognosis, diagnosis, and treatment of alcohol use disorders and other multifaceted disorders. These new approaches provide researchers with new …