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Investigation Of Novel Microseparation Techniques, Yansheng Liu

Theses and Dissertations

Ultrahigh pressure liquid chromatography (UHPLC) makes it possible to use very small particles (< 2 µm) as packing materials to provide high column efficiencies. Results from a careful comparison of small porous and nonporous particles show that when the particle size is small enough (< 2 µm), both porous and nonporous particles give excellent performance, and the differences in column efficiencies between porous and nonporous particles become insignificant. Columns packed with bare diamond particles could separate small molecules, especially polar molecules, however, severe tailing occurred for less polar compounds. The polybutadiene coated diamond particles gave greater retention and better separation of small molecules compared to bare particles, although no improvement in column efficiency was observed. Changes in surface bonding of thermally hydrogenated diamond particles was achieved by chemical modification using various organic peroxides with or without reagents containing long carbon chain functional groups. It appears that the alkyl groups were attached onto the diamond surface with limited coverage. LC experiments did not demonstrate good separation; however, changes in LC behavior were observed. A repetitive solvent programming approach was successfully applied to the analysis of a continuous sample stream in microbore LC. Each analysis cycle consisted of three steps: pseudo-injection, elution and rinse. In the pseudo-injection step, elution with a non- or poor-eluting solvent produced a concentrated sample plug due to on-column focusing. Factors influencing peak symmetry, resolution and analysis cycle length were investigated. Quantitative analysis of a continuous sample stream is possible under certain operating conditions. Electric field gradient focusing (EFGF) devices with distributed resistor substrates could focus proteins in the separation channel, however, the focused bands were not stable, and the repeatability was poor due to the formation of bubbles and pH gradient in the separation channel. Both fiber-based and porous glass capillary-based planar EFGF devices with changing cross-sectional area (CCSA) channels were constructed and evaluated with the aid of a home-made scanning laser-induced fluorescence detection system. The fiber-based CCSA EFGF devices gave poorer performance compared with glass capillary based devices. Porous glass capillary-based EFGF devices could focus single proteins and separate mixtures of two to three proteins.

The Plight Of Large Animals In Tropical Forests And The Consequences For Plant Regeneration, S. Joseph Wright, Kathryn E. Stoner, Noelle Beckman, Richard T. Corlett, Rodolfo Dirzo, Helene C. Muller-Landau, Gabriela Nuñez-Iturri, Carlos A. Peres, Benjamin C. Wang

Biology Faculty Publications

We introduce a special section that addresses the bushmeat or wild meat crisis, its direct impact on game species, and its indirect impact on plants in tropical forests.

Honors Convocation (Program 2007), Illinois Wesleyan University

The Kemp Foundation's Teaching Excellence Award Ceremony Programs

No abstract provided.

Newsroom, Georgia Southern University

Newsroom (2006-2023)

• Eight new members are named to Science and Technology Advisory Board

Volume 44, Number 66: April 17, 2007, University Of North Dakota

University Letter Archive

No abstract provided.

Phobias: The Evolutionary Trend, Grant Brown

Honors Capstones

Capstone submitted as a graduation requirement for the BSU Honors Program.

Unh Scientists, Students, Part Of Team Exploring New "Black Smoker" Undersea Chimney, David Sims

Media Relations

No abstract provided.

Smallpox Subunit Vaccine Produced In Planta Confers Protection In Mice, Maxim Golovkin, Sergei Spitsin, Vyacheslav Andrianov, Yuriy Smirnov, Yuhong Xiao, Natalia Pogrebnyak, Karen Markley, Robert Brodzik, Yuri Gleba, Stuart N Isaacs, Hilary Koprowski

Department of Cancer Biology Faculty Papers

We report here the in planta production of the recombinant vaccinia virus B5 antigenic domain (pB5), an attractive component of a subunit vaccine against smallpox. The antigenic domain was expressed by using efficient transient and constitutive plant expression systems and tested by various immunization routes in two animal models. Whereas oral administration in mice or the minipig with collard-derived insoluble pB5 did not generate an anti-B5 immune response, intranasal administration of soluble pB5 led to a rise of B5-specific immunoglobulins, and parenteral immunization led to a strong anti-B5 immune response in both mice and the minipig. Mice immunized i.m. with …

Cyberinfrastructure: Supporting Scientific Discovery And Innovation In The 21st Century, Jorge Diaz-Herrera

Presentations and other scholarship

No abstract provided.

Uab Biology Professor Wins 2007 Ireland Prize, Staff

Browse All News

No abstract provided.

Materials And Methods For Detection Of Enterovirus And Norovirus, John H. Paul, Iii, Erica T. Casper, Stacey S. Patterson

USF Patents

The invention provides polynucleotides and methods for detecting and quantifying RNA viruses, such as enteroviruses and noroviruses. In one aspect, the invention provides amplification primers and labeled molecular beacons for amplification of viral nucleic acid sequences. In another aspect, the invention provides a synthetic RNA internal control. In another aspect, the invention provides a kit for detecting the presence of enterovirus and/or norovirus in a sample.

Uaa Student Showcase Journal: Recognizing Excellence (2007), N/A N/A

UAA Student Showcase Journal

No abstract provided.

How The Devils Went Deaf: Ethnomycology, Cuisine, And Perception Of Landscape In The Russian North, Sveta Yamin-Pasternak

Anthropology

Arctic tundra is rich mushroom country and a number of high latitude fungi species can potentially be used as food. Different regions often play host to many of the same or similar mushroom varieties. Yet, people's attitudes toward the same mushrooms---and mushrooms in general---vary widely both in temporal and geographical senses. The given work presents a study in ethnomycology---a field of inquiry concerned with human beliefs and practices associated with mushrooms, carried out in the Bering Strait area of Chukotka, Russia. Once avoided by the Native people living on the Russian and American sides of the Bering Strait, wild mushrooms …

Agroborealis, Vol. 39, No. 1 (Summer 2007), School Of Natural Resources And Agricultural Sciences

Agroborealis

Forage & turf: the career of G. Allen Mitchell: Allen Mitchell's 36-year career has been devoted to finding better forage for animals, plants for revegetation, and good tough turfgrasses capable of withstanding Alaska's harsh winters-it's nice to have a good green for one's northern golf game. / Doreen Fitzgerald -- Wasps: the good, the bad, and the not-so-bad: Summer 2006 was a banner year for wasps in interior Alaska, much to the chargrin of picnic-goers-and with fatal consequences for two Fairbanksans. / Peter J. Landolt, Alberto Pantoja, Aaron Hagerty, Daryl Green, and Susan Emmert, with introduction by Deirdre Helfferich -- …

Evidence For A Northern Transitional Continental Margin Flora In The Cretaceous (Campanian To Maastrichtian) Matanuska Formation, Talkeetna Mountains, Southcentral Alaska, Sabra Louise Reid

Geosciences

The Late Cretaceous Matanuska Formation contains shallow and deep marine sediments and nonmarine sediment derived from the Talkeetna volcanic island arc. The sediment accumulated in the Matanuska Seaway, a tectonically active basin on the southern margin of southcentral Alaska. The Matanuska Seaway was contemporaneous with the Cretaceous Western Interior Seaway (CWIS) of North America. Angiosperm pollen taxa from the CWIS have been used to date units and reconstruct both paleolatitude and paleoclimate (Nichols and Sweet, 1993). Comparison of pollen taxa from the CWIS to assemblages from the Matanuska Formation reveals that outcrops at Mazuma Creek, Granite Creek, Syncline Mountain, and …

Developing Computer Models To Study The Effect Of Outdoor Air Quality On Indoor Air For The Purpose Of Enhancing Indoor Air Quality, Tomas Marsik

Engineering

People in developed countries spend the majority of their time indoors. Therefore, studying the effect of outdoor air quality on indoor air is of a great importance to human health. This thesis presents several dynamic computer models that were developed to study this effect. They estimate indoor pollutant levels based on outdoor levels, ventilation rate, and other factors. Also, an analysis method is presented that allows for quantifying the effect of outdoor air quality on indoor air at a given building based on measured real-time outdoor and indoor pollutant levels. An important part of this method is separating the measured …

Using Geophysical Constraints To Determine Groundwater Travel Times, Seafloor Arrival Locations, And Saltwater Concentrations For Transition Zone Depths At Underground Nuclear Detonations On Amchitka Island, Anna M. Wagner

Engineering

There is a great amount of radioactive material in the subsurface of Amchitka Island as a result from underground nuclear testing performed between 1965 and 1971. It is unknown how long it will take for the radionuclides to travel to the seafloor and the marine environment or where possible seepage zones will occur. The contaminant transport is greatly affected by the location of the transition zone (TZ) and the effective porosity, which were both determined by magnetotellurics (MT) in 2004. The hypothesis of this study was as follows: the groundwater travel times and seafloor arrival locations, can be estimated through …

Daily Eastern News: April 17, 2007, Eastern Illinois University

April

No abstract provided.

The Daily Gamecock, Tuesday, April 17, 2007, Uinversity Of South Carolina, Office Of Student Media

April

No abstract provided.

Agroborealis, Vol. 39, No. 2 (Winter/Spring 2007-2008), School Of Natural Resources And Agricultural Sciences

Agroborealis

Bonita J. Neiland remembered: The woman instrumental in founding the School of Natural Resources and Agricultural Sciences was a scientist ahead of her time. / Dorren Fitzgerald / Biomass for biofuels: not all trees are created equal: Alaska has vast stretches of forest: woody biomass that could be investigated for its potential as biofuel. Basic research is needed to determine the chemical composition and characteristics of the state's vast stores of the only renewable resource available capable of producing complex hydrocarbons. SNRAS researchers are conducting preliminary research into this potential through liquefaction of different tree species. / Andres Soria -- …

Six Thousand Years Of Change In The Northeast Pacific: An Interdisciplinary View Of Maritime Ecosystems, Nicole Misarti

Marine Biology

The goal of this thesis is to develop long-term records of North Pacific ecosystems and explore relationships between change in marine ecosystems and prehistoric Aleut culture through soil chemistry, isotope analyses of lake cores, and isotope analyses of bone from archaeological middens. Chemical analysis of soils yielded differences in soils of various archaeological features as well as middens of varying composition. Sites that had no middens were chemically distinguishable from sites that did have middens helping to define resource consumption in the local region. An important result of this study is that no single ecosystem (nearshore benthic, coastal pelagic or …

Reproductive Potential Of Pacific Cod (Gadus Macrocephalus) In Alaska, Olav Aleksander Ormseth

Marine Biology

The reproductive potential of female fishes, which results from the number of eggs they produce and the quality of individual eggs, is a critical factor in fisheries biology. Reproductive potential is important to individuals because maternal fitness is the product of the number of offspring produced and how many offspring survive. The growth rate of populations and their capacity for supporting commercial fisheries also depend on the number of viable offspring that females produce. I studied the reproductive potential of female Pacific cod (Gadus macrocephalus) in the North Pacific Ocean. Pacific cod is an important ecological and economic resource, yet …

Physical And Biological Factors Affecting The Diel Vertical Migration Of Walleye Pollock, Charles F. Adams

Marine Biology

The mechanisms underlying diel vertical migration (DVM) in marine fishes are unclear, although it is generally thought that this behavior is influenced by light, hydrography, food availability and predator avoidance. In the North Pacific Ocean, walleye pollock (Theragra chalcogramma) undergo DVM as juveniles, ascending to the surface at night and returning to the bottom at dawn. Adults are generally considered demersal. The objective of this research was to examine the effect of light, temperature and prey availability on the DVM of adult pollock. The work was undertaken to further our understanding of pollock biology, and the mechanisms underlying DVM in …

The Great Salmon Run: Competition Between Wild And Farmed Salmon, Gunnar Knapp, Cathy Roheim, James Anderson

ISER Reports

This report examines economic and policy issues related to wild and farmed salmon in North America. These issues have received a great deal of attention in recent years, reflecting the environmental, economic and cultural importance of salmon to Americans—and the fact that salmon issues span many important policy debates ranging from environmental protection to trade policy. The salmon industry has experienced dramatic change over the past two decades. Two major trends gave rise to many of the issues discussed in this report. The first trend is the rapid and sustained growth in world farmed salmon and salmon trout production, from …

Register For Summer Scholars, University Relations

Campus News Archive

For 22 years UMM has offered high school students an opportunity to attend "summer college” through the Summer Scholars program. UMM's two-week Summer Scholars program offers students a chance to earn two college credits and experience college life.

Foraging Ecology And Sociality Of Muskoxen In Northwestern Alaska, Claudia Ihl

Biological Sciences

I investigated sociality and winter foraging ecology of muskoxen ( Ovibos moschatus) in Cape Krusenstern National Monument, northwestern Alaska. The nutritional value of moss (Hylocomium splendens, Tomenthypnum nitens) for muskoxen was evaluated by incubating moss in rumen-fistulated muskoxen and simulating post-ruminal digestion by incubation in acid-pepsin. Moss was indigestible in muskoxen and gained mass and nitrogen in the rumen. Consequently, high moss consumption during winter may result in net loss of nitrogen from a muskoxen's system. Local and regional differences in moss use by muskoxen and caribou or reindeer (Rangifer tarandus) were investigated in northwestern Alaska in relation to indicators …

Circadian Rhythms, Neuroanatomy Of The Superchiasmatic Nucleus And Selective Breeding Of The Northern Red -Backed Vole (Clethrionomys Rutilus), Ronald J. Tavernier

Biological Sciences

The experiments performed in this thesis investigated the circadian rhythms and neuroanatomy of a subarctic rodent, the northern red-backed vole ( Clethrionomys rutilus). Arctic and subarctic light regimes are extreme, with long periods of light and dark and large daily changes in day-length, but very little is known about circadian rhythms of mammals at high latitudes. A colony of C. rutilus was established and proper husbandry techniques were developed to allow voles to reproduce in captivity. Wild-caught and laboratory reared animals were tested for circadian rhythms in a 16:8 hour light:dark (LD) cycle, constant dark (DD) and constant light (LL). …

Kids Count Alaska 2005, Virgene Hanna, Claudia Lampman

ISER Reports

Over the past 15 years, Alaska’s children as a group have gotten older, more racially diverse, and more international. The total number of children in Alaska increased about 11% between 1990 and 2004, but the number of children ages 9 and younger dropped 8% and the number ages 10 to 18 rose 40%. During the same period, the number of children from minorities—the largest minority being Alaska Native—increased 75%, while the number from immigrant families was up nearly half. This year we show a snapshot of Alaska children in foster care. These are mostly children the state Office of Children’s …

Southwest Alaska Network Long-Term Visitor Use Monitoring Protocol Development (Final Report), Steve Colt, Ginny Fay

ISER Reports

The purpose of this research is to assist the National Park Service (NPS), Southwest Alaska Network gain a better understanding of current visitor use volumes and patterns, develop a system to monitor visitor use over time, and use this information to evaluate the impact of visitors on the SW Network Park systems (Kenai Fjords National Park, Lake Clark National Park and Preserve, and Katmai/Aniakchak/Alagnak National Park and Preserve) as part of the NPS Vital Signs Monitoring program. Data about visitor use are important because of the driving force humans have on ecosystems. Not only are total numbers of visitors important …

Nest-Building Behavior In House Mice (Mus Musculus), A Potential Model Of Obsessive -Compulsive Disorder In Humans, Dana M. Greene-Schloesser

Biological Sciences

OCD (obsessive-compulsive disorder) is a chronic and debilitating psychiatric condition characterized by intrusive and persistent thoughts (obsessions) and repetitive behaviors (compulsions) that become ritualistic in an attempt to escape the obsessions. Currently there is a paucity of animal models with robust and spontaneous (non-drug or non-behaviorally induced) compulsive-like behaviors. This study is aimed at validating a novel robust and spontaneous genetic mouse model of OCD. The compulsive-like nest-building behavior in mice selected for high levels of nest-building behavior (BIG) has good face validity, with a behavioral phenotype that resembles hoarding behavior characteristic of OCD. In addition, male and female BIG …