Digital Commons Network^™

Sponges Of Navassa, Janie L. Wulff, Timothy D. Swain

Marine & Environmental Sciences Faculty Reports

This photographic guide was compiled from data collected during the 2004 NOAA survey of the coral reefs of Navassa and does not represent a comprehensive list of all Porifera in Navassa. Specifically missing are taxa that inhabit caves, overhangs, vertical walls; species that live in the interstices of the reef framework; and species found at depths greater than 50 meters. Specimens were identified by Janie Wulff and Timothy Swain of Florida State University using a combination of digital photography, field observations, and microscopic examination of siliceous spicules. Genera are organized into higher taxa according to Systema Porifera, Hooper & van …

Full Issue, Number 22, Spring 2006, The Congregational And Synodical Mission Unit, The Evangelical Lutheran Church In America

Intersections

No abstract provided.

A Church, The Human Condition, And The Fissured Face Of Peace, D. M. Yeager

Intersections

No abstract provided.

Sexuality Over The Lifespan--Social Trends Pose Moral Dilemmas For Communities Of Faith, Adina Nack

Intersections

No abstract provided.

Lutheran Education In The None Zone, Samuel Torvend

Intersections

No abstract provided.

Medicine@Yale May/June 2006 Volume 2, Issue 3, Yale School Of Medicine Office Of Communications

Medicine@Yale - development newsletter

Medicine@Yale : advancing biomedical science, education and health care was a print and online newsletter which published news about academics, research and philanthropy for alumni, donors, faculty, students and the general public. It was initially published 6 times a year and then changed to a quarterly schedule. The first issue came out June/Jul. 2005. The final issue was published in May/June 2022, vol. 17, issue 1. Published by Yale School of Medicine, Office of Communications.

Efeitos Da Radiação Ultravioleta-B Sobre A Morfologia Foliar De Arabidopsis Thaliana (L.) Heynh. (Brassicaceae), Maria Regina Torres Boeger, Mary Poulson

Biology Faculty Scholarship

Reduction of the ozone layer results in the increase in ultraviolet radiation reaching the earth’s surface, especially the ultraviolet-B (UV-B). The increase of radiation may induce structural and physiological changes in plants, influencing their growth and development. This paper evaluates the effects of ambient UV-B radiation upon to the leaf morphology of Arabidopsis thaliana developed under controlled conditions. The seeds of A. thaliana grown in environmental chamber, with 300 μmol m-2s-1 de photosynthetically active radiation (PAR) with and without 6 kJ m-2 s-1 of radiation UV-Bbe (UV-Bbe; UV-B biologically effective). After 21 days, ten leaves of each treatment (with and …

The Proliferation Security Initiative And The Evolution Of The Law On The Use Of Force, Mark R. Shulman

Elisabeth Haub School of Law Faculty Publications

No abstract provided.

Parity/Disparity: Electoral Gender Inequality On The Tightrope Of Liberal Constitutional Traditions, Darren Rosenblum

Elisabeth Haub School of Law Faculty Publications

Part I of this article examines Parity's strangeness to United States observers. United States sex discrimination law ignores political representation issues. United States voting rights law contains no provisions for gender inequality. Most importantly, leading United States thinkers of all stripes roundly reject quotas. Part II details the Parity debate and its relationship to French democracy. The democracies of the United States and of France share Eighteenth Century Enlightenment origins. They also share some form of universalism (labeled “neutrality” in the United States by Cass Sunstein) establishing the equality of all citizens before the law. Parity serves as a good …

A Phylogenomic Profile Of Globins, Serge N. Vinogradov, David Hoogewijs, Xavier Bailly, Raãºl Arredondo-Peter, Julian Gough, Sylvia Dewilde, Luc Moens, Jacques R. Vanfleteren

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Globins occur in all three kingdoms of life: they can be classified into single-domain globins and chimeric globins. The latter comprise the flavohemoglobins with a C-terminal FAD-binding domain and the gene-regulating globin coupled sensors, with variable C-terminal domains. The single-domain globins encompass sequences related to chimeric globins and «truncated» hemoglobins with a 2-over-2 instead of the canonical 3-over-3 α-helical fold.

Results

A census of globins in 26 archaeal, 245 bacterial and 49 eukaryote genomes was carried out. Only ~25% of archaea have globins, including globin coupled sensors, related single domain globins and 2-over-2 globins. From one to seven …

Gis-Based Analysis Of Ice-Breeding Seal Strandings In The Gulf Of Maine, David Harris Ph.D., Sat Gupta

Faculty Publications

Phoca groenlandica (harp seals) and Cystophora cristata (hooded seals), two species of ice-breeding seals, are being sighted more frequently onshore in the Gulf of Maine since 1990, but little is known about their behavior in this ecosystem. We obtained records of 904 ice-breeding seal stranding locations in the Gulf of Maine between 1996 and 2002 from NOAA Fisheries and used a geographic information system (GIS) to conduct group-wise comparisons by species (using non-parametric techniques), and to determine the predictors of high seal-stranding density (using ordinal logistic regression analysis). Compared to harp seals, hooded seals stranded closer to deep water, farther …

Measuring Inter-Rater Agreement For A School Psychology Case Study Rubric, Tracy Kay Spires

Graduate Theses and Dissertations

No abstract provided.

Water Issues During The First Term Of The Bush-Cheney Administration, Thomas L. Sansonetti

Wyoming Law Review

No abstract provided.

Federal R&D, Drug Discovery, And Pricing: Insights From The Nih-University-Industry Relationship, Wendy H. Schacht

Congressional Research Service Reports

Public interest in approaches that might provide prescription drugs at lower cost, particularly for the elderly, has rekindled discussion over the role the federal government plays in facilitating the creation of new pharmaceuticals for the marketplace. In the current debate, some argue that the government’s financial, scientific, and/or clinical support of health-related research and development (R&D) entitles the public to commensurate considerations in the prices charged for any resulting drugs. Others view government intervention in price decisions based upon initial federal funding as contrary to a long-term trend of government promotion of innovation, technological advancement, and the commercialization of technology …

Renal Damage In Experimentally-Induced Cirrhosis In Rats: Role Of Oxygen Free Radicals, Sathish Kumar Natarajan, Jayasree Basivireddy, Anup Ramachandran, Simmy Thomas, Prabhu Ramamoorthy, Anna B. Pulimood, Molly Jacob, Kunissery A. Balasubramanian

Department of Nutrition and Health Sciences: Faculty Publications

Cirrhosis with ascites is associated with impaired renal function accompanied by sodium and water retention. Although it has been suggested that mediators such as nitric oxide play a role in the development of renal failure in this situation, other mechanisms underlying the process are not well understood. This study examined the role of oxidative stress in mediating renal damage during the development of cirrhosis in order to understand mechanisms involved in the process. It was shown that carbon tetrachloride– or thioacetamide-induced cirrhosis in rats results in oxidative stress in the kidney as seen by increased lipid peroxidation and protein oxidation, …

Intestinal Mucosal Alterations In Rats With Carbon Tetrachloride-Induced Cirrhosis: Changes In Glycosylation And Luminal Bacteria, Sathish Kumar Natarajan, Prabhu Ramamoorthy, Simmy Thomas, Jayasree Basivireddy, Gagandeep Kang, Anup Ramachandran, Anna B. Pulimood, K. A. Balasubramanian

Department of Nutrition and Health Sciences: Faculty Publications

Spontaneous bacterial peritonitis is a major cause of mortality after liver cirrhosis. Altered permeability of the mucosa and deficiencies in host immune defenses through bacterial translocation from the intestine due to intestinal bacterial overgrowth have been implicated in the development of this complication. Molecular mechanisms underlying the process are not well known. In order to understand mechanisms involved in translocation of bacteria, this study explored the role of oxidative stress in mediating changes in intestinal mucosal glycosylation and luminal bacterial content during cirrhosis. CCl₄-induced cirrhosis in rats led to prolonged oxidative stress in the intestine, accompanied by increased …

Direct Detection Of Double-Stranded Dna: Molecular Methods And Applications For Dna Diagnostics, Indraneel Ghosh, Cliff I. Stains, Aik T. Ooi, David J. Segal

Cliff Stains Publications

Methodologies to detect DNA sequences with high sensitivity and specificity have tremendous potential as molecular diagnostic agents. Most current methods exploit the ability of single-stranded DNA (ssDNA) to base pair with high specificity to a complementary molecule. However, recent advances in robust techniques for recognition of DNA in the major and minor groove have made possible the direct detection of double-stranded DNA (dsDNA), without the need for denaturation, renaturation, or hybridization. This review will describe the progress in adapting polyamides, triplex DNA, and engineered zinc finger DNA-binding proteins as dsDNA diagnostic systems. In particular, the sequence-enabled reassembly (SEER) method, involving …

Sequence-Enabled Reassembly (Seer) Peptides For The Detection Of Dna Sequences, Aik T. Ooi, Cliff I. Stains, Jason R. Porter, Indraneel Ghosh, David J. Segal

Cliff Stains Publications

By combining custom zinc finger (ZF) DNA-binding technology [1,2] with protein fragment complementation [3], we have developed a technology, designated SEER (Sequence-Enabled Reassembly), that has the potential to “see” or detect genetic information within a living cell (Figure 1). These agents consist of two inactive parts of signal-generating peptides that have the ability to recognize specific DNA sequences. The two parts bind near each other in the presence of a user-defined DNA target site and generate a fluorescent signal. Two prototype SEER systems have been constructed, based on the reassembly of green fluorescent protein (SEERGFP) [4] and the enzyme β-lactamase …

A Dominant Complement Fixation Pathway For Pneumococcal Polysaccharides Initiated By Sign-R1 Interacting With C1q, Youngsun Kang, Yoonkyung Do, Haekyung Lee

Publications

The intricate system of serum complement proteins provides resistance to infection. A pivotal step in the complement pathway is the assembly of a C3 convertase, which digests the C3 complement component to form microbial binding C3 fragments recognized by leukocytes. The spleen and C3 provide resistance against blood-borne S. pneumoniae infection. To better understand the mechanisms involved, we studied SIGN-R1, a lectin that captures microbial polysaccharides in spleen. Surprisingly, conditional SIGN-R1 knockout mice developed deficits in C3 catabolism when given S. pneumoniae or its capsular polysaccharide intravenously. There were marked reductions in proteolysis of serum C3, deposition of C3 on …

The Roles Of Daf-6 And Cell-Cell Interactions In Sensory Organ Morphogenesis, Elliot A. Perens

Student Theses and Dissertations

The development of multicellular organs depends on the regulation of cell shape, position, and orientation. The genetic regulation of these morphogenetic processes is poorly understood. As a model for organ morphogenesis, I studied the development of the Caenorhabditis elegans amphid sensory organ. Sensory organs in diverse species are often composed of neuronal sensory endings accommodated in a lumen formed by ensheathing epithelia or glia. The generation of this structure may require cell-autonomous factors that control lumen formation, as well as cell non-autonomous factors that coordinate the morphogenesis of the lumen with the resident neuronal processes. Understanding these processes would provide …

Microarray Analysis Identifies Novel Cholesterol Regulated Genes, Including Pcsk9 Which Regulates Ldl Receptor Function And Ldl Cholesterol Levels, Kara Noelle Maxwell

Student Theses and Dissertations

Precise regulation of cholesterol levels is necessary for survival of individual cells and the normal functioning of multicellular organisms. Many of the genes important in these processes are regulated at a transcriptional level by the sterol regulatory element binding proteins (SREBPs) and liver X receptors (LXRs). This thesis describes the identification by microarray technology of novel genes in cholesterol metabolism and the characterization of two of these genes. Microarray analysis of the livers of mice fed a low versus high cholesterol diet identified 37 down-regulated and 32 up-regulated genes. Confirmation of these genes and analysis in transgenic and pharmacologically treated …

Nkains: A Novel Family Of Membrane Proteins That Interact With The Na, K-Atpase And Affect Membrane Excitability, Svetlana Gorokhova

Student Theses and Dissertations

A novel family of Na,K-ATPase interacting (NKAIN) proteins was cloned and characterized. These proteins are highly conserved across species and do not resemble any known proteins in the genome. Aside from the conserved transmembrane domains, NKAINs contain no known functional domains. Striking amino acid conservation in the first two transmembrane domains suggests that the function of these proteins is concentrated in the membrane bilayer. NKAIN1, 2, 3 and a splice form of NKAIN4 are brain and testis specific; another splice form of NKAIN4 is expressed ubiquitously. In the central nervous system, NKAINs localize to neurons. The C-terminal tail of NKAIN …

Telomere Structure And Shortening In Telomerase-Deficient Trypanosoma Brucei: Implications For Anitgenic Variation, Oliver Dreesen

Student Theses and Dissertations

A variety of parasites persist in their host through sequential expression of variant surface antigens. Intriguingly, the genes encoding these antigens are frequently found adjacent to the telomeres. Telomeres are nucleoprotein complexes consisting of tandem DNA repeats and proteins that bind to them. Their function is to protect chromosome ends from the DNA repair machinery that would otherwise recognize them as double-stranded breaks. With the help of the ribonucleoprotein telomerase they compensate for the gradual sequence loss that would otherwise arise from the inability of conventional DNA polymerases to replicate chromosome ends. In Trypanosoma brucei, the causative agent of African …

Integration Of Light And Temperature In The Regulation Of Circadian Gene Expression In Drosophila, Catharine E. Boothroyd

Student Theses and Dissertations

Clocks are aligned to the environment via inputs from both daily light and temperature cycles. Previous molecular and behavioral studies in Drosophila have largely focused on light-dependent regulation of circadian clocks and their outputs. Although light is the strongest and best understood Zeitgeber for the circadian clock, temperature is also an important factor. This thesis aims to understand better the role of temperature on gene expression and behavior in the fly, as well as to examine how information from both light and temperature are integrated by the clock to regulate circadian gene expression. Genome-wide expression profiles of transcripts from wild-type, …

Self-Renewal Requirements Of Human Embryonic Stem Cells And Their Engraftment Potential In Mouse Blastocysts, Daylon Jefferson James

Student Theses and Dissertations

Human embryonic stem cells (hESCs) are a unique population of cells derived from a 6 day old human embryo that can be maintained indefinitely in vitro and have the ability to differentiate to all adult cell types. In addition to their potential for cell based therapies in the treatment of disease and injury, the broad developmental capacity of hESCs offers potential for studying the origins of all human cell types. Embryonic stem cells were first derived from mouse embryos (mESCs), and years of work have demonstrated their utility to developmental research, but relatively little is known about human ESCs. The …

Exploring The Biophysical Properties Of Domains Within Multi-Domain Proteins, Vasant Muralidharan

Student Theses and Dissertations

The past few decades, considerable progress has been made in understanding the biophysical properties of proteins using small modular domains such as SH3 domains. However, there is a surprising lack of knowledge regarding how these properties are affected when the domain is placed back within its full-length multi-domain protein. Using a combination of expressed protein ligation (EPL) and in vivo amino acid replacement of tryptophans with tryptophan (Trp) analogues, we have developed an integrated approach that allows the domain-specific incorporation of optical probes into large recombinant proteins. The Src homology 3 (SH3) domain from the c-Crk-I adaptor protein has been …

Dendritic Cells Mediate The Dual Effects Of Ifn Alpha/Beta In Hcv Immunity, Randy Longman

Student Theses and Dissertations

Hepatitis C virus (HCV) infects an estimated 170 million people and is responsible for considerable morbidity and mortality worldwide. Successful resolution of infection is achieved naturally in approximately 30% of infected individuals and correlates with a strong T cell response. Furthermore, treatment with interferon (IFN)α achieves sustained virologic response in approximately 50% of chronically infected individuals. Conventional dendritic cells (cDCs), as the most potent antigen presenting cells, and plasmacytoid DCs (pDCs), as the principal IFNα/β producing cells, serve as potential targets for both immune evasion and therapeutic strategies. In order to evaluate the effects of HCV on DC function, we …

Studies On The Histone Methyltransferase G9a, Srihari Cidambi Sampath

Student Theses and Dissertations

The size and complexity of eukaryotic genomes require that specific mechanisms exist for ensuring both the stability as well as the accessibility of DNA. One such mechanism is the association of DNA with histones to form chromatin, the physiological substrate of gene expression. An important means by which histones impact transcriptional activity is through site-specific enzymatic modification of the amino terminal histone "tails", which can alter the spectrum of chromatin-associated proteins and hence transcriptional states. Among the known modifications of histones, lysine methylation has been proposed to represent a relatively stable mark which might mediate stable activation or repression, depending …

Structural Analysis Of An Rna Polymerase Sigma Factor And Its Anti-Sigma: Sigma28/Flgm, Margareta K. SöRenson

Student Theses and Dissertations

Eubacterial sigma (σ) factors are required during the initiation of transcription, where they play key roles in promoter recognition, melting, and transcriptional regulation, sigma is a dissociable subunit of RNA polymerase (RNAP), and sequence-specifically recognizes promoters, but only after associating with RNAP to form the holoenzyme. In the holoenzyme, the major sigma domains (sigma2, sigma3, and sigma4) are spread across the surface, with the promoter binding surfaces solvent exposed and appropriately positioned for simultaneous recognition of the -10 and -35 promoter elements. A ~30 residue linker connecting sigma3 and sigma4 is unfolded and threads through the interior of the enzyme. …

Medicine@Yale March/April 2006 Volume 2, Issue 2, Yale School Of Medicine Office Of Communications

Medicine@Yale - development newsletter

Medicine@Yale : advancing biomedical science, education and health care was a print and online newsletter which published news about academics, research and philanthropy for alumni, donors, faculty, students and the general public. It was initially published 6 times a year and then changed to a quarterly schedule. The first issue came out June/Jul. 2005. The final issue was published in May/June 2022, vol. 17, issue 1. Published by Yale School of Medicine, Office of Communications.