Digital Commons Network^™

Selenoproteins In Parasites, Gustavo Salinas, Alexei V. Lobanov, Vadim N. Gladyshev

Vadim Gladyshev Publications

Parasites, which cause an enormous burden in the population of the third world, are a diverse group of organisms, many of which are sensitive to oxidative stress imposed by their hosts. In recent years, several selenoprotein families, some with antioxidant properties, have been described and characterized in metazoan parasites. Glutathione peroxidase and thioredoxin glutathione reductase (TGR) appear to be essential selenoproteins in flatworms (phylum Platyhelminthes). TGR is the single enzyme that provides reducing equivalents to both thioredoxin and glutathione pathways, in contrast to hosts, which evolve parallel pathways. In roundworms (phylum Nematoda), selenoproteins have recently been described, revealing …

The 15-Kda Selenoprotein (Sep15): Functional Analysis And Role In Cancer, Vyacheslav M. Labunskyy, Vadim N. Gladyshev, Dolph L. Hatfield

Vadim Gladyshev Publications

The 15-kDa selenoprotein (Sep15) was identified several years ago as a protein of unknown function. In recent years, several lines of evidence implicated Sepl5 in the effect of dietary selenium in cancer prevention. These lines of evidence include: 1) protein expression patterns in normal and malignant cells; 2) identification of polymorphic sites that regulate Sep15 levels and differentially respond to selenium supplementation; 3) location of the Sep15 gene in the human genome; and 4) correlation between Sep15 haplotype and susceptibility to cancer. Functional analyses revealed a specific interaction between Sep15 and a protein folding sensor in the endoplasmic reticulum of …

Mammalian And Other Eukaryotic Selenocysteine Trnas, Bradley A. Carlson, Xue-Ming Xu, Rajeev Shrimali, Aniruddha Sengupta, Min-Hyuk Yoo, Robert Irons, Nianxin Zhong, Dolph L. Hatfield, Byeong Jae Lee, Alexei V. Lobanov, Vadim N. Gladyshev

Vadim Gladyshev Publications

Selenocysteine (Sec) tRNA occupies a prominent position in the expression of selenoproteins as it is essential for their synthesis and it provides the means by which selenium is co-translationally inserted into protein as the amino acid, Sec. Thus, Sec tRNA is regarded as the principle constituent in selenoprotein synthesis. Many features unique to this tRNA have been characterized over the years in mammals and other eukaryotes. In the last five years, the major advances have been in an elucidation of the different roles that the two major Sec tRNA isoforms play in selenoprotein biosynthesis and in Sec biosynthesis. One isoform …

Prevalence Of Autoantibodies To Various Tissue Antigens Before And During S2 – Complex Immunotherapy In Head And Neck Cancer Patients, Batool M. Mahdi

Iraqi Postgraduate Medical Journal

ABSTRACT : BACKGROUND: A total of 63 terminal untreatable stage IV head and neck cancer patients were investigated for clinical responses and presence of autoantibodies to various tissue antigens before and during S2- complex immunotherapy. METHODS: S2 –complex is a new low molecular weight biological response modifier (BRM) with a potant immunostimulating and anti tumor activities. RESULTS: Autoantibodies detected at pretreatment period were those directed towards the following antigens : nuclear, thyroid microsomal, epithelial cells, gastric parietal cells, smooth muscles, peripheral leukocytes, T-lymphocytes, B-lymphocytes, monocytes, thymus reticular epithelial cells and Hassall’s corpuscles. Beside, autoantibodies with specificities to glomerular basement membrane …

Evaluation Of Amino Acid Homocysteine In Hypertensive Patients, Ali Taqi. Al-Baldawi

Iraqi Postgraduate Medical Journal

ABSTRACT : BACKGROUND : Hypertension is a world wide disease and in 90% of cases the cause is unknown . Its early detection and treatment can prevent serious complications such as ischemic heart disease (IHD) . Furthermore the association of lipids with IHD is a well-known fact . However abnormally high levels of homocysteine were found to be strongly linked to an increase risk of coronary artery disease . OBJECTIVES: To evaluate the total plasma homocysteine concentration in hypertensive patients. METHODS : Total plasma homocystein concentrations were measured using High Performance Liquid Chromatography(HPLC) with Ultraviolet( UV) detector in 60 hypertensive …

The Prevalence Of Silent Gall Stones And Its Relation To Some Risk Factors In Iraq, Safa M. M. Al-Obaidi

Iraqi Postgraduate Medical Journal

ABSTRACT: BACK GROUND : Gallstone disease is a common condition all over the world as well as in Iraq. Symptomatic gallstones definitely need surgical or medical treatment . To remove or treat silent gallstones is still a debatable subject. Gallstones are about twice as common in women than in men and their incidence vary according to some physical fasters METHODS : 1016 adult volunteers from both sexes and of different age groups were examined by ultrasound in Medical city teaching hospital between January 2004 and August 2005, for the estimation of the prevalence of silent gallstones among Iraqi people and …

Epstein- Barr Virus In Iraqi Patients With Nasopharangeal Carcinoma, Batool Mutar Mahdi

Iraqi Postgraduate Medical Journal

ABSTRACT: BACKGROUND: Epstein-Barr virus (EBV) was ubiquitous Herpes virus that had a role in the development of undifferentiated carcinoma of the nasopharynx, Burkett’s lymphoma, acute infectious mononucleosis and other lymphoprolifrative disorders. METHODS: Thirty Iraqi patients with nasopharangeal carcinoma were referred to Oncology Unit in Al-Kadhemia Teaching Hospital from 1992-1994. Sera of those patients were tested for the presence of antibodies against Epstein-Barr virus nuclear and early antigens using indirect immunoflourescence test. Cellular immunity for those patients was tested for the CD4+, CD8+, CD4/CD8 ratio, T-cells % and B -cells %. Their results were compared with twenty-two normal apparently normal individuals. …

The Location Of Aerobactin Determinants Of Uropathogenic E. Coli In Association With Hemolysin Production Antibiotic Resistance And Patient Characteristics., Ibtesam Ghadban Auda

Iraqi Postgraduate Medical Journal

ABSTRACT: BACKGROUND: Aerobactin, Hemolysin and the resistance to some antibiotics axe important Features of Uropathogenic E. coli (UPEC). The characteristics of patients from which we isolate the UPEC have associate with these Features and their determinants location. AIM: We determined the phenotypic expression and gene location of Aerobactin and phenotypic expression of Hemolysin among 60 UPEC isolates. We correlated the presence of Aerobactin system with antibacterial agents resistance. The expression of Hemolysin and the characteristics of patients. METHODS: Two methods of plasmid curin, sodium dodecyl sulfate and elevated temperature plasmid curing, are used then plasmid extraction and agarose gel electrophoresis …

The Role Of Organizational Culture In The Delivery Of Humanitarian Assistance And Inter-Organizational Collaboration, Jamie Antoinette Wilke Corvin

USF Tampa Graduate Theses and Dissertations

The international community has seen a dramatic increase in the complexity of aid operations, with increasing numbers of donors participating in humanitarian aid endeavors. Therefore, it is essential to determine factors that contribute to successful aid delivery. In an effort to guide more effective and efficient aid operations, this study investigated organizational culture in three distinct types of humanitarian organizations: (a) the U.S. military, (b) the International Federation of the Red Cross, and (c) a sampling of local and indigenous non-governmental organizations. Being a relatively unexplored area, this study took an exploratory approach, utilizing qualitative methods to analyze humanitarian aid …

The Effect Of The Aqueous Extract Of Peganum Harmala Seeds On The Mammary Glands Of Virgin, Pregnant, And Lactating Rats, Wasan Al- Saidi

Iraqi Postgraduate Medical Journal

ABSTRACT: BACKGROUND: Penganum harmala is a well known herb that is used by different societies. It is used as a medical herb in treating various diseases and disorders .There was no earlier published work on the effects of the aqueous extract of Peganum harmala . Therefore, we designed this study to investigate the effect of the aqueous extract of Peganum harmala seeds on the mammary gland at the various physiological states (virgin, pregnancy and lactation) making use of the available histological, histochemical, and immunohistochemical means. METHODS: Aqueous extract of Peganum harmala was given for two weeks by an orogastric tube …

Protection Of Endangered Species: Sturgeon: Struggle For Survival Has Become Critical, Natalia M. Restivo

Cornell Law School J.D. Student Research Papers

This research is dedicated to the current situation and problems of sturgeon species in Russia, mainly in the Caspian Sea region. In particular, the paper elaborates on the endangered beluga sturgeon. The work describes characteristic features of sturgeon, points out the current problems and proposes some suggestions on preservation efforts in order to improve the critical situation. The research carried out by the author includes a brief consumer survey which exhibits the level of awareness and indicates willingness of the society to maintain the stability and provide sustainable development of the sturgeon species. The author favors and encourages intrinsic value …

Perspective Greenhouse Gas Mitigation By Covers On Livestock Slurry Tanks And Lagoons?, Soren O. Petersen, Daniel N. Miller

Department of Animal Science: Faculty Publications

Liquid manure (slurry) storage facilities are important point sources of atmospheric pollution. Being point sources, containment of gaseous emissions via improved storage conditions may be possible, and permeable surface covers (natural crusts and artificial covers) are increasingly recognized for their capacity to reduce various gaseous emissions. Microbial transformations in permeable surface covers include bacterial-methane oxidation, but this interface between nitrogen-rich slurry and the atmosphere is also an environment with intense nitrogen turnover that can lead to nitrous oxide emissions. Both methane and nitrous oxide are greenhouse gases, and strategies to reduce environmental impact of slurry stores must consider the total …

Lanthanide Complexes As Fluorescent Indicators For Neutral Sugars And Cancer Biomarkers, Onur Alptürk, Oleksandr Rusin, Sayo O. Fakayode, Weihua Wang, Jorge O. Escobedo, Isiah M. Warner, William E. Crowe, Vladimir Král, Jeff M. Pruet, Robert M. Strongin

Faculty Publications

Simple water-soluble lanthanum and europium complexes are effective at detecting neutral sugars as well as glycolipids and phospholipids. In solutions at physiologically relevant pH the fluorescent lanthanum complex binds neutral sugars with apparent binding constants comparable to those of arylboronic acids. Interference from commonly occurring anions is minimal. The europium complex detects sialic acid-containing gangliosides at pH 7.0 over an asialoganglioside. This selectivity is attributed, in large part, to the cooperative complexation of the oligosaccharide and sialic acid residues to the metal center, based on analogous prior studies. In MeOH, lysophosphatidic acid (LPA), a biomarker for several pathological conditions including …

Is A Shutdown Of The Thermohaline Circulation Irreversible?, Jianjun Yin, Michael E. Schlesinger, Natasha G. Andronova, Sergey Malyshev, Bin Li

Faculty Publications

The Atlantic thermohaline circulation (THC) plays a vital role in explaining past abrupt climate changes and in maintaining the current climate. Its remarkable nonlinear dynamics, first demonstrated by H. M. Stommel, have been supported by various types of climate models. This has led to severe concerns that global warming may shut down the THC irreversibly, with consequent catastrophic climate changes, particularly for Europe. Here we use an uncoupled ocean general circulation model (OGCM) and a coupled atmosphere-ocean general circulation model (AOGCM) to investigate the nonlinear response of the THC to freshwater perturbations in the northern North Atlantic. We find that …

Implications For Cognitive Quantum Computation And Decoherence Limits In The Presence Of Large Extra Dimensions, Jonas R. Mureika

Physics Faculty Works

An interdisciplinary physical theory of emergent consciousness has previously been proposed, stemming from quantum computation-like behavior between 10⁹ or more entangled molecular qubit states (microtubulin). This model relies on the Penrose-Diósi gravity-driven wavefunction collapse framework, and thus is subject to any secondary classical and quantum gravity effects. Specifically, if large extra spatial dimensions exist in the Universe, then the resulting corrections to Newtonian gravity cause this model to suffer serious difficulties. It is shown that if the extra dimensions are larger than 100 fm in size, then this model of consciousness is unphysical. If the dimensions are on the …

Illinois Wesleyan Biologists Hope To Continue Antarctic Research, Ann Aubry

News and Events (Discontinued Series)

No abstract provided.

Genetic Code Based Coding And Mathematical Formulation For Dna Computation, Mingjun Zhang, Maggie Xiaoyan Cheng, Tzyh-Jong Tarn

Computer Science Faculty Research & Creative Works

DNA computation is to use DNA molecules for information storing and processing. Challenges currently faced by DNA computation are (1) lack of theoretical computational models for applications, and (2) high error rate for implementation. This paper attempts to address these problems from genetic coding and mathematical modeling aspects. The proposed genetic coding approach provides a promising alternative to reduce high error rate. The mathematical formulation lays down groundwork for studying theoretical aspects of DNA computation.

Anadromous Rainbow Smelt And Tomcod In Connecticut: Assessment Of Populations, Conservation Status, And Need For Restoration Plan, Heather A. Fried, Eric T. Schultz

EEB Articles

(beginning of rainbow smelt executive summary)

Evidence indicates that anadromous rainbow smelt (Osmerus mordax) populations in Connecticut and elsewhere in the northeast United States have severely declined. Several sampling programs have documented declines in Connecticut’s smelt populations over the last three decades (Marcy 1976a, Marcy 1976b, Millstone Environmental Laboratory 2005). Similar declines have also been documented in the Hudson River (ASA Analysis & Communication 2005) and in Massachusetts (personal communication, Brad Chase, MA Division of Marine Fisheries 2004). Recreational and commercial fisheries in the region for this species have virtually ceased (Blake and Smith 1984). The Connecticut Fish Advisory Committee …

June 26, 2006 Reporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Rhythm Method As Cause Of Embryonic Death Based On Flawed Assumptions, Richard J. Fehring

College of Nursing Faculty Research and Publications

Response to "The rhythm method and embryonic death" by L. Bovens in Journal of Medical Ethics, Vol. 32 (2006): 355-356.

Patterns Of Habitat Use By Primates In Eastern Ecuador, Seema Nayan Sheth

Theses

Lowland tropical rain forests of western Amazonia are characterized by the most speciose primate communities in the Neotropics, immediately leading to the question of to what extent does niche partitioning by primate species serve as a mechanism to promote species co-existence. Because the primate assemblages that we observe today reflect a combination of ecological and evolutionary processes, this study examines habitat occupancy and its relationship to phylogeny and space in a diverse diurnal primate community in an undisturbed lowland rain forest of Amazonian Ecuador. Specifically, the following null hypotheses are explored as potential factors that shape community structure: (1) mean …

What Business Reference Librarians Need To Know About Intellectual Property: Presentations Sponsored By The Patent And Trademark Depository Library Association (Ptdla) At The American Library Association Annual Conference, New Orleans, June 25, 2006, Nan Myers, Jan Comfort, Virginia A. Baldwin, Donna Hopkins

University of Nebraska-Lincoln Libraries: Presentations

Presentations sponsored by the Patent and Trademark Depository Library Association (PTDLA) at the American Library Association Annual Conference, New Orleans, June 25, 2006

Speaker #1: Nan Myers Associate Professor; Government Documents, Patents and Trademarks Librarian Wichita State University, Wichita, KS

Title: Intellectual Property Roundup: Copyright, Trademarks, Trade Secrets, and Patents

Abstract: This presentation provides a capsule overview of the distinctive coverage of the four types of intellectual property – What they are, why they are important, how to get them, what they cost, how long they last. Emphasis will be on what questions patrons ask most, along with the answers! …

Multivariate Analysis And Visualization Of Splicing Correlations In Single-Gene Transcriptomes, Mark C. Emerick, Giovanni Parmigiani, William S. Agnew

Johns Hopkins University, Dept. of Biostatistics Working Papers

Through ‘combinatorial splicing’, RNA metabolism may create enormous structural diversity in the proteome. Functional interactions among multiple alternative domains can have a disproportionate impact on the phenotype, requiring integrated RNA-level regulation of molecular composition. Splicing correlations within molecules expressed from a single gene, where these effects would be greatest, provide valuable clues to functional relationships and targets for splicing regulation. We present tools to visualize complex splicing patterns in full-length cDNA libraries. Developmental changes in pair-wise correlations are presented vectorially in ‘clock plots’ and linkage grids. Higher-order correlations are assessed via a loglinear model and Monte Carlo analysis with an …

The Environment Transport Of Radium And Plutonium: A Review By Brice Smith And Alexandra Amonette, Institute For Energy And Environmental Research (Ieer), Brice Smith, Alexandra Amonette

Institute for Energy and Environmental Research (IEER)

In this report we will provide a brief review of the environmental transport of two specific radionuclides. In Chapter Two we will consider the mobility of radium. This naturally occurring radionuclide is part of the uranium and thorium decay series, and is thus a potential concern in many areas where these elements have been mined or processed. In addition to the large number of sites with radium bearing waste, we chose to focus on this radionuclide in part due to the high concentrations of radium-226, and its thorium-230 parent, in the raffinate waste from the former Fernald Feed Material Production …

The Importance Of The Strictly Conserved, C-Terminal Glycine Residue In Phosphoenolpyruvate Carboxylase For Overall Catalysis: Mutagenesis And Truncation Of Gly-961 In The Sorghum C4 Leaf Isoform, Wenxin Xu, Shaheen Ahmed, Hideaki Moriyama, Raymond Chollet

Hideaki Moriyama Publications

Phosphoenolpyruvate carboxylase (PEPC) is a “multifaceted,” allosteric enzyme involved in C4 acid metabolism in green plants/microalgae and prokaryotes. Before the elucidation of the three-dimensional structures of maize C4 leaf and Escherichia coli PEPC, our truncation analysis of the sorghum C4 homologue revealed important roles for the enzyme’s C-terminal α-helix and its appended QNTG⁹⁶¹ tetrapeptide in polypeptide stability and overall catalysis, respectively. Collectively, these functional and structural observations implicate the importance of the PEPC C-terminal tetrapeptide for both catalysis and negative allosteric regulation. We have now more finely dissected this element of PEPC structure-function by modification of the absolutely conserved …

We Are...Marshall, June 23, 2006, Office Of Marshall University Communications

We Are ... Marshall: the Newsletter for Marshall University 1999-2023

No abstract provided.

The Electronic Health Record In Practice: Why, How, And What Next?, Lisa Sprague, Sally Coberly

National Health Policy Forum

This local site visit was intended to allow participants to observe the electronic health record (EHR) as used in practice by two U.S. leaders in technology and quality, the Veterans Health Administration (VHA) and Kaiser Permanente (KP). The VHA has employed an EHR system since 1997; KP is in the process of implementing a standard system for all clinicians nationwide. The site visit was designed to provide an opportunity for participants to explore both the expected benefits from EHR adoption and the specific lessons these two large, integrated delivery systems have learned in their transition from paper to electronic records. …

A Morphometric Analysis Of The Geomorphology Of Florida's Springs, Andrew Curtis Walker

KIP Theses and Dissertations

An exploratory study of the geomorphology of Florida's springs was conducted using morphometric analysis. Spatial datasets for spring locations, elevation data, physiography, geology and hydrography were acquired for incorporation and analysis with GIS technology.

Red Rock Desert Learning Center: Evaluation Of Curriculum: Final Report, Lori Olafson, Gregory Schraw

Reports (RRLC)

The UNLV Center for Evaluation and Assessment developed an assessment instrument to evaluate curriculum for the proposed Red Rock Desert Learning Center science school’s fifth grade program. A 25-item Likert-type scale was created and administered before and after 68 pre-service and in-service teachers participated in each of five curriculum pilot sessions. The survey consisted of questions related to knowledge, pedagogy, and attitudes. Two open-ended questions were included on the post-survey.

Findings revealed significant gains in knowledge, pedagogy, and attitudes in each of five events from pre- to post-test. Gains over time were greatest for knowledge. Some events were rated more …

Geologic Map And Structural Analysis Of The Twin Rocks 7.5 Minute Quadrangle, Wayne County, Utah, Samuel C. Sorber

Theses and Dissertations

A new geologic map of the Twin Rocks 7.5 minute quadrangle primarily located within Capitol Reef National Park, south-central Utah, provides stratigraphic and structural detail not previously available. This map has also been instrumental in understanding the evolution and development of fluvial terraces associated with Sulfur Creek and the structural geology of the backlimb of the Miners Mountain uplift. Nine bedrock stratigraphic formations and eight types of Quaternary deposits were mapped throughout the quadrangle. Bedrock stratigraphy ranges in age from Permian to Jurassic. New details absent on previous geologic maps include members of the Chinle and Moenkopi Formations and the …