Digital Commons Network^™

Methods For Obtaining And Analyzing Whole Chloroplast Genome Sequences, Robert K. Jansen, Linda A. Raubeson, Jeffrey L. Boore, Claude W. Depamphilis, Timothy W. Chumley, Rosemarie C. Haberle, Stacia K. Wyman, Andrew J. Alverson, Riannon Peery, Sallie J. Herman, H. Matthew Fourcade, Jennifer V. Kuehl, Joel R. Mcneal, James Leebens-Mack, Liying Cui

All Faculty Scholarship for the College of the Sciences

During the past decade there has been a rapid increase in our understanding of plastid genome organization and evolution due to the availability of many new completely sequenced genomes. Currently there are 43 complete genomes published and ongoing projects are likely to increase this sampling to nearly 200 genomes during the next five years. Several groups of researchers including ours have been developing new techniques for gathering and analyzing entire plastid genome sequences and details of these developments are summarized in this chapter. The most important recent developments that enhance our ability to generate whole chloroplast genome sequences involve the …

Becoming The Bridge: Border-Crossing, Intersectionality, And Wave Theory In Contemporary Feminist Movement, Meagan Lynn Weatherby

Renée Crown University Honors Thesis Projects - All

The self-proclaimed “third wave” ofUnited Statesfeminism, which rose from the abyss of “postfeminist” 1980s conservatism in the early 1990s, is a vibrant movement that is quickly picking up the pace of feminist organizing in the new millennium. Third wave feminists envision themselves as taking up the torch of the 1960-1970s “second wave” of feminism, but expanding upon it by emphasizing intersectionality (the complex interaction of multiple oppressions, e.g. sexism, racism, classism) and making room for sexiness, joy, and fun in feminist organizing and theorizing. It is a discourse that strives toward and privileges intersectional analyses that create space in which …

Contesting Gender In Popular Culture And Family Law: Middlesex And Other Transgender Tales, Susan Frelich Appleton

Indiana Law Journal

No abstract provided.

Leslie Meek Receives Tate Award For Advising, University Relations

Campus News Archive

No abstract provided.

Assessment Of General Education Survey Report, 2005, Assessment Of Student Learning Committee

Assessment of Student Learning Reports

No abstract provided.

The Discourse Beneath: Emotional Epistemology In Legal Deliberation And Negotiation, Erin Ryan

Scholarly Publications

All lawyers negotiate, and all negotiators deliberate. This article addresses the pervasive but unrefined use of emotional insight by deliberating and negotiating lawyers, and suggests that legal education could improve lawyering by adopting a fuller model of legal thinking that takes account of this "epistemological emotionality." In forming the beliefs that underlie choices made during deliberation and negotiation, people rely on insights informed by past and present emotional experience. Such epistemological emotionality fuels a pre-linguistic, quasi-inductive reasoning process that enables us to draw on stored information about emotional phenomena to hypothesize about motives, behavior, and potential consequences. As deliberation moves …

Hibernation Is Recorded In Lower Incisors Of Recent And Fossil Ground Ssquirrels (Spermophilus), Tom Goodwin, Gail R. Michener, Daniel Gonzalez, Caroline E. Rinaldi

Faculty Publications

Incremental dentin and associated enamel, features visible on the surface of lower incisors, were characterized for 3 species of ground squirrels (Spermophilus): Pleistocene and Recent S. elegans, Recent S. richardsonii, and Recent S. parryii. A hibernation mark was evident in incisor dentin and enamel, most characteristically as a sleeve of enamel terminating basally adjacent to medially depressed dentin with indistinct and often very fine increments. This mark was absent in juveniles but present in older animals of both sexes for at least 6 weeks after hibernation, eventually being lost through growth and wear of the incisor. Temporal association with hibernation …

The Effects Of Nutrient Concentrations On Macroinvertebrate Distributions In Georgia, Paula Michele Pollock Brossett

Theses and Dissertations

Nutrients are considered the second largest nonpoint source pollutant in Georgia's running water ecosystems. Nutrients can naturally occur in low amounts and are typically increased in streams as a result of industry, agriculture, silviculture, and urbanization. In large amounts, nutrients can cause many problems to occur in the stream ecosystem. Macroinvertebrates have been established as good indicators for determining the level of impairment of a stream; however, not much research has been done on how nutrients affect macroinvertebrates in a stream. Approximately 225 reference and impaired streams were sampled over a 3 - year field season, (index period from September …

Guidelines For Recommending Precision Agriculture In Southern Crops, Susan Watson, Eduardo Segarra, Robert Lascano, Kevin Bronson

Journal of Extension

Technology has tremendous implications for Extension agents working with producers, agribusinesses, and youth. Four southern crops, including cotton, corn, grain sorghum, and peanuts, were evaluated under current agricultural management practices and precision farming technology. Yield, profit, and fertilizer application levels are compared across the two management practices. Field characteristics for the most profitable locations are outlined as a reference for producers in determining whether they would likely be good candidates for this technology. Results are commodity specific and suggest maximum bounds on investment levels that would be profitable to producers.

Morphological And Molecular Defects In Zebrafish Embryos (Danio Rerio) After Chromated Copper Arsenate Exposure, Kerry Jean Lee

Biological Sciences Theses & Dissertations

This investigation identified the morphological defects caused by different concentrations of chromium, copper, and arsenic (CCA) on the developing zebrafish embryo; in addition to defects caused by the three combined components (complete CCA). A change in the DNA of the developing embryos in response to the three components of CCA and complete CCA was also determined. Early Dania rerio embryos were cultured for five days with various concentrations of CCA components and complete CCA. Morphological defects were assessed with light microscopy and DNA fragmentation was determined by agarose gel electrophoresis. Embryonic exposure of D. rerio to sub lethal concentrations of …

Correlation And Causes Of Fifth Order Cycles Within The Upper Cretaceous Eagle Formation, Bighorn Basin Of Wyoming, Kimberly Ann Johnson

OES Theses and Dissertations

Cyclic stratification was examined in the Upper Cretaceous (Santonian-Campanian) section (Eagle Formation) within the Bighorn Basin of Wyoming. Of particular concern was the 10³ to 10⁴ year band, which in marine settings has been attributed to orbital forcing (Milankovitch cyclicity). A series of 19 sections were measured through the Virgelle Member of the Eagle Formation. Most were measured on the “J”-shaped escarpment that constitutes the nose and north flank of the Thermopolis anticline. Several others were measured at sites up to 30 km to the northwest along paleo-shoreline. In the study, detailed thickness data of all sections and …

Cultural Competence And Curricula In Physical Therapist Professional Education, Beth Ernst Jamali

Theses and Dissertations in Urban Services - Urban Education

The purpose of this research was to examine the methods and degree to which content related to cultural competence is incorporated into current entry-level physical therapist education. Face-to-face interviews were performed with 10 program directors from various physical therapist education programs across the country to obtain in-depth information regarding cultural competence in the physical therapy curricula. A questionnaire was sent to all of the 193 accredited physical therapist education programs in the United States as a second means to collect information regarding the methods and extent to which cultural competence is included in the physical therapy curricula. Data were collected …

The Hilltop 4-1-2005, Hilltop Staff

The Hilltop: 2000 - 2010

No abstract provided.

Role Of A Conserved Glutamate Residue In The Escherichia Coli Seca Atpase Mechanism, Christopher R. Zito, Edwin Antony, John F. Hunt, Donald B. Oliver, Manju M. Hingorani

Biological Sciences Faculty Research and Publications

Escherichia coli SecA uses ATP to drive the transport of proteins across cell membranes. Glutamate 210 in the “DEVD” Walker B motif of the SecA ATP-binding site has been proposed as the catalytic base for ATP hydrolysis (Hunt, J. F., Weinkauf, S., Henry, L., Fak, J. J., McNicholas, P., Oliver, D. B., and Deisenhofer, J. (2002) Science 297, 2018–2026). Consistent with this hypothesis, we find that mutation of glutamate 210 to aspartate results in a 90-fold reduction of the ATP hydrolysis rate compared with wild type SecA, 0.3 s^–1versus 27 s^–1, respectively. SecA-E210D also releases ADP …

Methods For Fabricating Microarrays Of Motile Bacteria, Sergey Rozhok, Clifton K. -F. Shen, Pey-Lih H. Littler, Zhifang Fan, Chang Liu, Chad A. Mirkin, Richard C. Holz

Chemistry Faculty Research and Publications

Motile bacterial cell microarrays were fabricated by attaching Escherichia coli K-12 cells onto predesigned 16-mercaptohexadecanoic acid patterned microarrays, which were covalently functionalized with E. coli antibodies or poly-L-lysine. By utilizing 11-mercaptoundecyl-penta(ethylene glycol) or 11-mercapto-1-undecanol as passivating molecules, nonspecific binding of E. coli was significantly reduced. Microcontact printing and dip-pen nanolithography were used to prepare microarrays for bacterial adhesion, which was studied by optical fluorescence and atomic force microscopy. These data indicate that single motile E. coli can be attached to predesigned line or dot features and binding can occur via the cell body or the flagella of bacteria. Adherent bacteria …

Carbon Nanotubes And Tungsten Oxide Nanorods: Synthesis And Applications, Bing Xiao

Electrical & Computer Engineering Theses & Dissertations

Synthesis and applications of two types of one-dimensional nanomaterials, carbon nanotubes (CNTs) and tungsten oxide nanorods, are investigated in this dissertation. Multi-walled CNTs have been successfully synthesized using two types of chemical vapor deposition (CVD) methods: microwave plasma enhanced CVD and atmospheric pressure thermal CVD. CNTs and their synthesis processes are characterized with various analysis techniques including scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and optical emission spectroscopy. Ultra-thin and high quality multi-walled CNTs are discovered in CNT films produced by MPCVD, which exhibit good field emission performance that is found to be dependent on the synthesis …

Eternal Sunshine Of The Spotless Mind And The Question Of Transcendence, David L. Smith

Journal of Religion & Film

The screenplays of Charlie Kaufman explore possibilities of transcendence within the limits of a wholly natural world. From Being John Malkovich through Eternal Sunshine of the Spotless Mind, his characters struggle to transform their lives or to get beyond the limits of their circumstances. Most of these quests end in paradox, but some, especially in Kaufman's most recent films, point in more promising directions. In this paper, I interpret Eternal Sunshine as offering something very much like Nietzsche's concept of eternal recurrence as a way to transform the conditions of life from within.

Kinsey, Chad Wilkes

Journal of Religion & Film

This is a review of Kinsey (2004).

Traveling "To The Farthest Reaches Of The Ada," Or Taking Aim At Employment Discrimination On The Basis Of Perceived Disability?, Michael D. Reisman

Cardozo Law Review

No abstract provided.

Periodicals Price Survey 2005: Choosing Sides, Lee Van Orsdel, Kathleen Born

Scholarly Papers and Articles

No abstract provided.

The Straggling Green's Function Method For Ion Transport, Steven Andrew Walker

Mathematics & Statistics Theses & Dissertations

For many years work has been conducted on developing a concise theory and method for HZE ion transport capable of being validated in the laboratory. Previous attempts have ignored dispersion and energy downshift associated with nuclear fragmentation and energy and range straggling. Here we present a Green's function approach to ion transport that incorporates these missing elements. This work forms the basis for a new version of GRNTRN, a Green's function transport code. Comparisons of GRNTRN predictions and laboratory results for an ⁵⁶Fe ion beam with average energy at the target of one GeV/amu or more are presented for …

Predictors Of Student Success In An Entry-Level Baccalaureate Dental Hygiene Program, Mohammad J. Alzahrani

Dental Hygiene Theses & Dissertations

The purpose of this study was to measure the utility of a variety of predictors that have been used by the Old Dominion University, Gene W. Hirschfeld School of Dental Hygiene and other baccalaureate dental hygiene programs in selecting dental hygiene students who are most likely to graduate and be successful in passing the National Board Dental Hygiene Examination (NBDHE). Specifically this study attempted to determine whether or not the following factors are reliable predictors of success in a an entry-level baccalaureate dental hygiene program as evidenced by graduation and successful entry into the profession as evidenced by passing the …

An Examination Into The Effects Of Social Positioning And Capitalist Immersion On Gendered Attitudes Towards Homosexuality In The United States, Fiona J. Blee

Sociology & Criminal Justice Theses & Dissertations

Previous research on attitudes towards homosexuality has concentrated on characteristics of individuals, and neglected the capitalist structure of society that provides the contextual setting for the creation and maintenance of cultural attitudes.

This thesis uses responses collected in the general social survey of the United States between 1993 and 1998, to provide a more comprehensive explanation of differences in attitudes towards homosexuality, through an analysis of social position and the capitalist agenda. The analysis revealed significant relationships between sex, age, race, income, beliefs regarding the domestication of women, and attitudes towards homosexuality.

The analysis showed that respondents categorized as black …

The Defensin Gene In Dermacentor Variabilis And Ixodes Scapularis, Katherine Corbett Seguin

Biological Sciences Theses & Dissertations

In response to microbial challenge, invertebrates rely on their innate immune response to recognize and destroy harmful pathogens. One of the primary proteins produced by the response and that has been observed in many invertebrates, including insects, mussels, and ticks, is defensin. These proteins are responsible for forming pores in the membranes of invading microbes thereby destroying them. This study examined the gene coding region for defensin in two important tick disease vectors, Dermacentor variabilis and Ixodes scapularis. PCR amplification of the genes from tick chromosomal DNA resulted in the amplification of a 225 base pair amplicon. Sequencing of …

Statistical Analysis Of Longitudinal And Multivariate Discrete Data, Deepak Mav

Mathematics & Statistics Theses & Dissertations

Correlated multivariate Poisson and binary variables occur naturally in medical, biological and epidemiological longitudinal studies. Modeling and simulating such variables is difficult because the correlations are restricted by the marginal means via Fréchet bounds in a complicated way. In this dissertation we will first discuss partially specified models and methods for estimating the regression and correlation parameters. We derive the asymptotic distributions of these parameter estimates. Using simulations based on extensions of the algorithm due to Sim (1993, Journal of Statistical Computation and Simulation, 47, pp. 1–10), we study the performance of these estimates using infeasibility, coverage probabilities of the …

Feeding Behavior And Conditioning In Two Heterotrophic Dinoflagellates, Todd A. Egerton

Biological Sciences Theses & Dissertations

Growth and abundance of heterotrophic dinoflagellate predators are generally attributed to the availability of algal prey. Several species of dinoflagellates feed on a wide variety of food types including fish. However the actual feeding preferences of dinoflagellates have been much less studied. In the few studies that have been carried out on dinoflagellate feeding preference, none have looked at possible factors that may affect preference. I conducted three experiments on the toxic dinoflagellate Pfiesteria piscicida and the related unnamed species Cryptoperidiniopsis brodyi which, (1) calculated the feeding preferences between algal Rhodomonas prey and fish blood cells and identified factors that …

A Systems Methodology For Measuring Operational Organization Effectiveness: A Study Of The Original Equipment Computer Manufacturing Industry, 1948 To 2001, Teddy Steven Cotter

Engineering Management & Systems Engineering Theses & Dissertations

Optimizing operational organizational effectiveness is the central, although often unstated, goal of engineering management and systems engineering research and applications. Two fundamental problems remain to be addressed in pursuit of this goal. First, despite over fifty years of research in various disciplines, there is still no universally accepted definition of organizational effectiveness. Second, no methodology exists to identify the domains, dimensions, and determinants of operational organizational effectiveness and dynamically model operational organizational effectiveness within a given population.

This research synthesizes a systems engineering methodology for identifying the domains, dimensions, and determinants of and dynamically modeling operational organizational effectiveness for an …

The Role Of Interfunctional Market Orientation In New Product Program Success: An Exploratory Empirical Analysis, George Grady Gresham

Theses and Dissertations in Business Administration

A central tenet of the market orientation construct is the need for virtually all departments, not just the marketing department, to participate in gathering, disseminating and responding to market intelligence. Previous academic research provides empirical support for a positive relationship between market orientation and superior business performance. Superior business performance, for many firms, depends upon a continuous stream of new products emerging from their product development programs, yet few studies have demonstrated the link between market orientation and new product development program success. This dissertation conceptualizes market orientation below the strategic business level as interfunctional market orientation (IFMO) for cross-functional …

Mechanisms Of Cell Death Initiated In Herpes Simplex Virus Thymidine Kinase Expressing Colon Tumor Cells Treated With Ganciclovir And Ucn-01, Christina Elizabeth Ahn

Theses and Dissertations in Biomedical Sciences

Metastatic colon carcinoma is the second leading cause of death from malignancy in the United States, and development of more effective treatments is essential. Heterologous expression of Herpes Simplex Virus Thymidine Kinase (HSVtk) in combination with the prodrug, ganciclovir (GCV), has shown great promise for the genetic therapy of many cancers, but most patients have had only a partial or minimal response to the therapy. After screening a panel of two drug combinations, our laboratory has shown that the combination of GCV and the protein kinase inhibitor UCN-01 (7-hydroxystaurosporine) enhances tumor cell death more effectively than either drug alone. However …

Developing And Optimizing Conditions For Single Cell Genetic Analysis, Susan Adele Gitlin

Theses and Dissertations in Biomedical Sciences

Preimplantation Genetic Diagnosis (PGD) allows for couples to obtain genetic information at the embryo stage, therefore preventing lethal genetic diseases in their offspring. A recent multi-center study determined a 17% amplification failure rate overall and a 3.4% misdiagnosis rate (ESHRE PGD consortium steering committee, 2002). Experiments presented here were conducted to decrease those rates by optimizing single cell sensitivity and specificity of PGD techniques. Primer extension preamplification (PEP) was used after initial amplification failure, followed by gene specific PCR. Reamplification experiments yielded 54% amplification rates using blastomeres that previously failed amplification. In the clinical trial, four of six blastomeres were …