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Dendritic Cells In Transplantation - Friend Or Foe?, Robert I. Lechler, Wan F. Ng, Ralph M. Steinman

Publications

The existence of two sets of dendritic cells in transplantation (of donor and recipient origin) poses unique problems in alloimmunity and tolerance. Donor DCs are potent stimulators of the direct pathway, in which recipient T cells respond to peptides presented on donor MHC products or to the donor MHC molecules themselves. Reduced DC function in the direct pathway is used to explain the acceptance of certain allografts that have been depleted of passenger leukocytes. Reciprocally, purified donor DCs powerfully stimulate the rejection of these grafts as well as the mixed leukocyte reaction by purified allogeneic T cells in culture. Donor …

Mature Dendritic Cells Infected With Canarypox Virus Elicit Strong Anti-Human Immunodeficiency Virus Cd8+ And Cd4+ T-Cell Responses From Chronically Infected Individuals, Jose Engelmayer, Marie C. Larsson, Andrew W. Lee

Publications

Recombinant canarypox virus vectors containing human immunodeficiency virus type 1 (HIV-1) sequences are promising vaccine candidates, as they replicate poorly in human cells. However, when delivered intramuscularly the vaccines have induced inconsistent and in some cases transient antigen-specific cytotoxic T-cell (CTL) responses in seronegative volunteers. An attractive way to enhance these responses would be to target canarypox virus to professional antigen-presenting cells such as dendritic cells (DCs). We studied (i) the interaction between canarypox virus and DCs and (ii) the T-cell responses induced by DCs infected with canarypox virus vectors containing HIV-1 genes. Mature and not immature DCs resisted the …

T Cell Studies In A Peptide-Induced Model Of Systemic Lupus Erythematosus, Magi Khalil, Kayo Inaba, Ralph M. Steinman

Publications

We have previously reported that immunization with a peptide mimetope of dsDNA on a branched polylysine backbone (DWEYSVWLSN-MAP) induces a systemic lupus erythematosus-like syndrome in the nonautoimmune BALB/c mouse strain. To understand the mechanism underlying this breakdown in self tolerance, we examined the role of T cells in the response. Our results show that the anti-foreign and anti-self response induced by immunization is T cell dependent and is mediated by I-E^d-restricted CD4⁺ T cells of the Th1 subset. In addition, generation of the critical T cell epitope requires processing by APCs and depends on the presence of …

Dendritic Cells: Specialized And Regulated Antigen Processing Machines, Ira S. Mellman, Ralph M. Steinman

Publications

[No abstract available]

Functions Of The Rat's Temporal Association Cortex In Higher Perceptual Functions, Summer L. Maki

The Corinthian

The role of the rodent temporal cortex in relational perception was investigated. Eighteen Long Evans rats were separated into three surgical groups (sham, partial striate, and Te2/3) and trained on a preoperative and postoperative discrimination task. The rats were then presented a series of relational judgement trials consisting of both between category judgments and within category judgments. It was hypothesized that the rats with Te2/3 lesions would perform as well as controls on the preoperative and postoperative discrimination task, but show a considerable behavioral dysfunction on the tests of visual relational perception. The results showed no differences across groups in …

Reflection And Remembrance: Oral Histories And Critical Thinking, Robin O. Harris, John Pilgrim, Jennifer Puckett, Teresa Pike, Jill Simmons, Paula Berenguer, Brad Brinkley, Ivan Bracic, Keshae Cobb, Chandler Rushing, Shannon Kitchens, Karen Hall

The Corinthian

The research assignment for this class was to interview someone who lived through World War II. The interviewee could be someone who served in the military service, someone on the home front or even a child-anyone who was touched by the experience of World War II.

Review Of Akira Mizuta Lippit, Electric Animal: Toward A Rhetoric Of Wildlife., Claudia Baracchi

Bryn Mawr Review of Comparative Literature

Akira Mizuta Lippit, Electric Animal: Toward a Rhetoric of Wildlife. Minneapolis: University of Minnesota Press, 2000. vii + 287 pp. ISBN 0816634858 (hb), 0816634866 (pbk).

Thrown Away Like Rubbish - Disposal Of The Dead In Ancient Greece, Astrid Lindenlauf

Classical and Near Eastern Archaeology Faculty Research and Scholarship

In this article, the literary and archaeological evidence for burial practices that can be associated with the English expression ‘to be disposed of like rubbish’ are discussed. These disposal methods (átaphon rhíptesthai) include the exposure of corpses to carrion animals, to the elements, as well as the disposal of corpses into the sea, pits or natural fissures without burial rites. They also include cases in which graves were dug up in order to throw their contents out. Here, the Greek expressions for the English phrase ‘to be thrown away like rubbish’ are explored, as well as its relation …

And Unto Dust Return: The Remembered Earth, Lawrence Kimmel

Philosophy Faculty Research

The earth is a primal resource of human imagination; its conceptual and creative tie to literature is pervasive, in part, because of the profound ambiguity of our relationship to it. Home and prison, earth holds in bondage the life it sustains. The paradox of life as freedom and life as bondage gives rise to the conflicting task of holding to the good earth, yet becoming free of it. This paradox and conflicting effort forms a basic pattern in western thought. A deep ambivalence and generative tension frames metaphors in literature from the earliest mythic and creation stories to the most …

Reconciliation And Harmony: The Philosophical Art Of Tragic Drama, Lawrence Kimmel

Philosophy Faculty Research

In the performance of art one can begin at the beginning, but in a discussion of art one must begin somewhere in the middle. Here, it is with the conviction that art, in whatever form, though it may surprise the sense and quicken the spirit, disturb our thinking or revoke a thoughtless ease, still, its full expression restores a sense of presence and wholeness to our being. That is, every art form has a point of closure in a harmony of the spirit. Even tragic drama, which brings the darkness of human character into a glare of recognition and acceptance, …

Jeffrey Friedman, 2000, The Rockefeller University

Harvey Society Lectures

Jeffrey M. Friedman. Leptin and the regulation of body weight

Posted with permission

Aquilegia, Vol. 25 No. 4, May-June 2001: Newsletter Of The Colorado Native Plant Society, Leo P. Bruederle, Bill Jennings, David Steinmann, Miriam Denham, Ronald L. Hartman, Kacey Conway, Carolyn Crawford, Janet L. Wingate

Aquilegia - Colorado Native Plant Society

No abstract provided.

Aquilegia, Vol. 25 No. 6, September-October 2001: Newsletter Of The Colorado Native Plant Society, Tina Jones, Rob Udall, Myrna P. Steinkamp, Tamara Naumann, Jim Borland, Janet Wingate, Jim Locklear

Aquilegia - Colorado Native Plant Society

No abstract provided.

The Effect Of Space And Furnishings On Academic Readiness Scores Of West Virginia Preschoolers, J. Elise Coccari

Theses, Dissertations and Capstones

The effect of space and furnishings, as measured by the Early Childhood Environment Rating Scale – Revised (ECERS-R), on the School Readiness Composite (SRC) of the Bracken Basic Concept Scale –Revised (BBCS-R) was investigated. The data analyzed was collected as part of the West Virginia Educare Initiative and included the SRC from 65 preschool students, ranging in age from 2 years, 6 months to 5 years, 0 months. The first subtest score on the ECERS-R, space and furnishings, was also calculated for each child’s classroom. The Spearman rank correlation coefficient between the SRC on the BBCS-R and the space and …

A Durkheimian Analysis Of Anomie And Deviance In The National Football League: An Exploratory Case Study, Eric M. Carter

Theses, Dissertations and Capstones

The purpose of this study was to determine whether Durkheim’s concept of anomie applies to National Football League players. This study was an attempt to discover if and why NFL players are more likely to commit deviant acts. Why do these players that seemingly have everything throw their lives away by committing crimes? This study attempts to address these questions by linking Durkheim’s classic anomie theory with the deviance in the NFL.

In Durkheim’s book, The Division of Labour in Society (1893), anomie emerges through society’s transition from mechanical to organic solidarity. In this, economic change is too fast for …

Natural Succession Of Benthic Populations In Constructed Sediment Ponds And Ditches In Southwestern West Virginia, Robin E. Dolin

Theses, Dissertations and Capstones

Strip mining causes major disturbances of the natural environment. One such disturbance is the creation of valley fills, which often fill in the headwaters of small streams. Runoff from these valley fills can cause heavy siltation as well as acid and heavy metal deposition downstream. One way to combat this problem is through the construction of sedimentation ponds, which slow down the flow of water so that sediment can settle out and water chemistry can be altered before the water is discharged into the stream. Since these ponds are, in effect, temporarily replacing small headwaters once present, the question is …

Analysis Of E. Coli Dna From Selected Source(S) Using Pulsed-Field Gel Electrophoresis, Sheema Doshi

Theses, Dissertations and Capstones

The presence of fecal coliforms in surface waters is a public health issue throughout the United States and Canada. The presence of Escherichia coli (E. coli) indicates recent fecal contamination from either animal or human sources. To solve this growing problem one must identify the source(s) of such contamination by creating a database of DNA profiles of£. coli isolates. Hence, tracking the source(s) of E. coli can help link water contamination to animal source(s) of contamination. In this study, pulsed field gel electrophoresis (PFGE) was used to analyze DNA from E. coli isolates from known animals. For PFGE, the bacterial …

The Narratology Of Jennifer Johnston's Novels, Robert N. Hutton

Theses, Dissertations and Capstones

Irish novelist Jennifer Johnston has published twelve novels to date, from The Captains and the Kings in 1972 to The Gingerbread Woman in 2000. Eileen Battersby's recent Irish Times article “Making Sense of Life” called her “the quiet woman of Irish fiction, “ referring to her understated, sophisticated writing style. All of her novels are short (Joseph Connelly and others have called them “novellas”), and she has become known for her ability to describe a complex situation in a direct, compact way.

This discussion is intended to investigate the narratology of several Johnston novels: to explore narrative voice, narrative chronology, …

Factors Influencing Spatial Variability In Soil Nitrogen (N) Dynamics In N-Treated And Untreated Watersheds Of The Fernow Experimental Forest, West Virginia, Nikki Lenore Lyttle

Theses, Dissertations and Capstones

The central Appalachian region of the United States receives some of the highest inputs of nitrogen (N) due to acidic deposition in the nation. It is believed that these high could levels contribute to a decline in forest soils within the next 50 to 70 yrs. This study examines factors that influence spatial variability in N-treated and untreated watersheds of the Fernow Experimental Forest, Parsons, West Virginia. Within each of the two watersheds [WS4 untreated control, > 100 yr.; WS3 N-treated, acidified, clear cut, ~ 31 yr.], two 0.04 ha plots, one high N and one low N, were selected for …

Methods Development For The Identification Of Unknown Proteins, Laura Brumfield Neal

Theses, Dissertations and Capstones

The chemistry of transport, storage, energy production, and reproduction in physiological systems is controlled by the actions and interactions of proteins. A species full complement of these amazing machines is often referred to as its proteome. The new science of proteomics utilizes a set of powerful and evolving tools to characterize the nature of the proteome, and further to fully elucidate the functional attributes of the proteins and their associations.

A methodology was developed by combining several proteomic techniques, such as electrophoresis, RP-HPLC, and mass spectrometry, to correctly identity proteins. Three known proteins (myoglobin, albumin, and actin) were employed for …

A Site Specific Impact Study: Valley Fill Construction Impacts On Water Chemistry And Benthic Macroinvertebrate Assemblages, Terry Morton Tomasek

Theses, Dissertations and Capstones

Downstream impacts of contour surface mining and valley fill construction were evaluated utilizing both water chemistry and EPA approved Rapid Bioassessment Protocol III. Wiley Branch was sampled in February of 1999 before mining perturbation. Five downstream stations were established with benthic macroinvertebrates and water samples collected seasonally for a period of sixteen months. Water chemistry parameters, pH, total aluminum, iron, and manganese, total suspended solids, total dissolved solids, sulfates, alkalinity, acidity and specific conductivity fluctuated but generally remained within acceptable governmental limits. Initially, benthic macroinvertebrate communities were well balanced within the stream. There was a high abundance of EPT individuals …

Characterization And Regulation Of Skn-Directed Autoimmune Skin Lesions, Pamela Jane Staton

Theses, Dissertations and Capstones

In the Skn model of autoimmunity, pathogenic T cells sensitized to Skn epidermal antigens (Skn-immune spleen cells or SIS cells) elicited disease in the form of skin lesions when adoptively transferred into shaved immunoincompetent syngeneic adult recipients (‘lesion-forming animals’) while concomitant injection of immunoregulatory normal spleen cells at twice the concentration (2 x normal spleen cells or 2XNS cells) of SIS cells significantly lessened lesion development (‘lesion-controlling animals’) by Day 7 post cell transfer. In a time-course RT-PCR cytokine analysis of skin from lesion-forming and lesion-controlling animals, IL-7 mRNA was significantly elevated in lesion-controlling skin on Day 7 while no …

Cognitive And Emotional Variables In Childhood Anxiety, Robin L. Browning

Theses, Dissertations and Capstones

Research was conducted on 60 subjects from mental health agencies in order to determine that childhood anxiety and depression can be differentiated. The 60 subjects were given the CDI and the RCMAS self-report measures in order to be placed in groups. This allowed the subjects to be divided into 4 groups: Anxious, Anxious/Depressed, Depressed and Not Anxious/Not Depressed. The 4 groups were then given 2 additional self-report measures, the STAIC and FSSC -R to measure whether Anxious and the Anxious/Depressed children exhibited more fears and anxious traits than did the Depressed and Not Anxious/Not Depressed children. Analyses of variance revealed …

Distribution, Morphometries, And Tooth Morphology Of The Genus Desmognathus In West Virginia, Jessica Ann Wooten

Theses, Dissertations and Capstones

Very few genera of vertebrates demonstrate variation in body size, color, pattern, and life history traits to the extreme that is demonstrated in the genus Desmognathus. Exact distribution of Desmognathus, as well as other genera of salamanders, is very difficult to outline because of hybridization between certain desmognathine species. Tooth morphology, tail keeling, head and body shape, and geographic location are factors used in the separation among species in this genus. There are five desmognathine salamanders, including D. monticola, D. fuscus, D. ochrophaeus, D. quadramaculatus, and 0. welteri in West Virginia. The purpose of this study was to analyze the …

A Natural History Study Of Desmognathus Welteri In West Virginia, Zachary Ira Felix

Theses, Dissertations and Capstones

The Black Mountain salamander, Desmognathus welteri, is a relatively large brownish-green salamander that possesses morphological characters associated with a semi-aquatic lifestyle. The history of this species in the state of West Virginia is an interesting one. Due to taxonomic confusion and the lack of a thorough investigation of the species in the state, little was known about the species until recently. Surveys for Desmognathus welteri in southern West Virginia revealed five new populations. This makes 17 localities for the species in the state. It appears D. welteri is restricted to relatively high elevation, high gradient, forested streams in the Cumberland …

Oral History Interview: Pearl Swann Carter, Pearl Swann Carter

0064: Marshall University Oral History Collection

This interview is one of series conducted concerning Oral Histories of African-American women who taught in West Virginia public schools. Pearl Swann Carter began teaching at a school in Clintonville, West Virginia. She gives us detailed information about her family throughout the interview, including her husband and children, a relative who was a slave, a story about her sister becoming mayor of White Sulphur Springs, and organizations her husband and her family belonged to. She attended Bethune school, Bolling High School[?], and West Virginia State, and then graduate school (which includes Marshall University); she gives us information about them, including …

Gardner-Webb Review, Volume 3, 2001, Jackson Ranier

Gardner-Webb Review

Annual publication associated with the Life of the Scholar Multidisciplinary Conference. This publication presents selected essays written by undergraduate students at Gardner-Webb University.

Foodways

Publications and Exhibits

This series features essays, biographical sketches, photographs, and recipes exploring food and community life in Knox County. Topics include gardening, hunting and trapping, food markets, the economics of food, new farmers, feeding the hungry, eating out, ritual food, cooking, canning and preserving, food and healing, and food choices. The series is based on extensive field research.

A New Species Of Extinct Oryzomyine Rodent From The Quaternary Of Curaçao, Netherlands Antilles, Donald A. Mcfarlane, Adolphe O. Debrot

WM Keck Science Faculty Papers

An extinct new species of Oryzomyine rodent, known since 1936 from the cave of Grot van Hato on the island of Curaçao, is described from abundant new specimens collected from owl-pellet deposits at three localities on the island.

The International Legal Rights Of Indigenous Peoples Affected By Natural Resource Exploitation: A Brief Case Study, Martin Wagner

UC Law SF International Law Review

No abstract provided.