Digital Commons Network^™

Absence Of Tuberculosis In Free-Ranging Deer In Nebraska, David J. Steffen, David W. Oates, Mauritz C. Sterner, Vickie L. Cooper

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Lymph nodes from 271 white-tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus) in Nebraska (USA) were examined microscopically for tuberculoid lesions. Lymph nodes lesions in at least one node were found in 12 deer. Lesions were examined with Zeihl-Neelson acid fast stains and by polymerase chain reactions using M. bovis specific probes. No evidence of tuberculosis was found. The small granulomatous lesions were likely caused by other bacteria.

Meningeal Worm In Free-Ranging Deer In Nebraska, David W, Oates, Mauritz C. Sterner, David J. Steffen

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The meningeal worm (Parelaphostrongylus tenuis) was found in 22 (7%) of 300 white-tailed deer (Odocoileus virginianus) (257 adults, 43 fawns) examined from Nebraska (USA) during November 1996. None of 53 mule deer (Odocoileus hemionus) (47 adults and 6 fawns) examined were infected. Twenty-two white-tailed deer from 18 counties in eastern Nebraska were infected with Parelaphostrongylus tenuis. This is the first record of P. tenuis in white-tailed deer from this state.

Evolutionary Relationships Among The Protostrongylidae (Nematoda: Metastrongyloidea) As Inferred From Morphological Characters, With Consideration Of Parasite-Host Coevolution, Ramon A. Carreno, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The phylogeny of nematodes in the family Protostrongylidae (Nematoda: Metastrongyloidea) was reconstructed by cladistic analysis of 28 binary and multistate characters derived from comparative morphology. Analyses were hierarchical, and examined (1) relationships among genera, including 13 ingroup taxa and Metastrongylidae as an outgroup (single tree, 78 steps, consistency index [CI]=0.705); and (2) relationships among genera and species groups including 21 ingroup taxa and Metastrongylus apri as an outgroup (single tree, 76 steps, CI=0.582). In the species-level tree, Protostrongylidae was divided into 2 major clades, one containing the subfamilies Muelleriinae (including the recently described Umingmakstrongylus pallikuukensis) Elaphostrongylinae, and the Varestrongylinae …

Experimental Infections Of Muskoxen (Ovibos Moschatus) And Domestic Sheep With Umingmakstrongylus Pallikuukensis (Nematoda: Protostrongylidae): Parasite Development, Population Structure, And Pathology, Susan J. Kutz, Eric P. Hoberg, Lydden Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Three captive muskoxen (Ovibos moschatus) were successfully infected with third-stage larvae of Umingmakstrongylus pallikuukensis digested or emerged from the slugs Deroceras reticulatum and D. laeve, for the first time completing the life cycle of this parasite under experimental conditions. The course of parasite development and patency was followed for 26 months post infection (p.i.) using fecal examinations and radiography. The prepatent periods in two of the muskoxen were 91 and 95 days and the patent period in one extended for 23 months. Larval production peaked 13–14 months p.i. On postmortem of two of the muskoxen at months …

Acanthocephala Of Cichlids (Pisces) In Lake Malawi, Africa, With A Description Of Acanthogyrus (Acanthosentis} Malawiensis Sp. N. (Quadrigyridae) From Labeo Cylindricus Peters, 1852 (Cyprinidae), Omar M. Amin, Sherman S. Hendrix

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Two species of acanthocephalans are reported from fishes collected during the summer of 1996 in the southeast arm of Lake Malawi (Lake Nyasa), East Africa. The common Acanthogyrus (Acanthosentis) tilapiae (Baylis, 1948) had infected 18 cichlid and 1 bagrid host species (all new host records) netted by divers wearing scuba gear around Harbor Island or caught by hook and line in deep water. The specimens of this parasite were some of the smallest ever reported for that species. Two males of a new species, Acanthogyrus (Acanthosentis) malawiensis, are described from 2 male Labeo cylindricus (Cyprinidae). This is the fifth …

Affinity Chromatography: A Review Of Clinical Applications, David S. Hage

David Hage Publications

Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLCbased methods. Some traditional applications of this approach include the use of boronate, lectin, protein A or protein G, and immunoaffinity supports for the direct quantification of solutes. Newer techniques that use antibody-based columns for on- or off-line sample extraction are examined in detail, as are methods that use affinity chromatography in combination with …

Scienceworks: A University-Based Science Outreach Group, Rochelle Payne Ondracek, Diandra Leslie-Pelecky

Diandra Leslie-Pelecky Publications

ScienceWorks is a science and engineering outreach group at the University of Nebraska- Lincoln, comprised of faculty, graduate students, undergraduates and community volunteers. We are brought together by one goal - to make science fun and accessible for everyone. ScienceWorks has developed over 30 modules that emphasize a range of scientific and engineering topics and guide the participants in discovery-based learning. Modules cover a wide variety of topics, including pressure, materials, electromagnetism, archaeology, DNA, optics and fracture. Most ScienceWorks modules involve hands-on activities. In Electromagnetism for example, students are asked to use a length of wire and a nail to …

Octamer-Based Genome Scanning Distinguishes A Unique Subpopulation Of Escherichia Coli O157:H7 Strains In Cattle, Jaehyoung Kim, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

Multilocus-genotyping methods have shown that Escherichia coli O157:H7 is a geographically disseminated clone. However, highresolution methods such as pulse-field gel electrophoresis demonstrate significant genomic diversity among different isolates. To assess the genetic relationship of human and bovine isolates of E. coli O157:H7 in detail, we have developed an octamer-based genome-scanning methodology, which compares the distance between over-represented, strand-biased octamers that occur in the genome. Comparison of octamer-based genome-scanning products derived from >1 megabase of the genome demonstrated the existence of two distinct lineages of E. coli O157:H7 that are disseminated within the United States. Human and bovine isolates are nonrandomly …

Ontogenetic Shifts In Canion Attractiveness To Brown Tree Snakes (Boiga Irregularis), John A. Shivik, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

The brown tree snake (Boiga irregularis) is a nocturnal, primarily arboreal, rear-fanged colubrid native to parts of Australasia (Savidge, 1987; Greene, 1989). Throughout their range, brown tree snakes eat a variety of prey including lizards, rats, and birds (Greene, 1989; Shine, 1991; Rodda, 1992; Rodda et al., in press). Brown tree snakes on Guam have a wide diet consisting mainly of lizards and lizard eggs, but a variety of other items were found in snake stomachs, including odd items such as cooked spareribs (Savidge, 1988). Savidge (1988) noted an ontogenetic shift in Guam brown tree snake diets; small …

Capsicum-Laden Soils Decrease Contact Time By Northern Pocket Gophers, Ray T. Sterner, Kelly Hollenbeck, Stephen Shumake

United States Department of Agriculture Wildlife Services: Staff Publications

Fossorial rodents damage lawns/water impoundments/ crops. We conducted a two-choice, parametric-type study to determine the effects of capsicum-oleoresin/soil mixtures (0.00, 0.75, 1.50, and 2.25%) upon soil-contact, soil-digging, and pelage-grooming behaviors in northern pocket gophers (Thomomys talpoides). In 3 alternate-day (1-h/day) exposures to ≥1.50% capsicum-oleoresin soil mixtures, gophers decreased mean soil contact time by 46% relative to placebo-exposed animals. Grooming time yielded a concentration x trial interaction that showed intense grooming by capsicum-exposed animals during trial 1, with "convergence" of times to near those of the "placebos" (0.00% capsicum oleoresin) by trial 3. The significant decrease in grooming activity …

Trapping Considerations For The Fossorial Pocket Gopher, Gary W. Witmer, Rex Marsh, George Matschke

United States Department of Agriculture Wildlife Services: Staff Publications

Fossorial mammals such as the pocket gopher (Thomomys spp.) are well adapted to life in an underground closed burrow system. These animals can have considerable influence on ecosystems and can cause significant damage to agricultural crops, forest resources, and field machinery. The research and management of pocket gopher populations poses many challenges to land managers and research biologists. Both live-trapping for research purposes and kill trapping for control purposes are important tools for working with pocket gophers. Trapping can be an effective and efficient tool if the proper traps are used and trappers are trained to place traps properly …

Effective Of Preventive Coyote Hunting On Sheep Losses To Coyote Predation, Kimberly Wagner, Michael Conover

United States Department of Agriculture Wildlife Services: Staff Publications

Aerial hunting is commonly used by agriculture agencies in the Intermountain West to reduce coyote (Canis latrans) predation on domestic sheep. We assessed the effect of aerial hunting of coyotes on sheep losses to coyotes, and the need for corrective predation management (hours of work, device nights) on the same pastures when sheep arrived for the subsequent summer grazing season (3-6 months after aerial hunting). Comparisons were made between paired pastures with (treated) and without (untreated) winter aerial hunting from helicopters. Average (x ± SE) pasture size was 45.2 ± 14.1 km² (n = 21) for treated …

Repellents To Reduce Cable Gnawing By Northern Pocket Gophers, Stephen A. Shumake, Ray T. Sterner, Stanley E. Gaddis

United States Department of Agriculture Wildlife Services: Staff Publications

There is a continuing need to develop improved repellent formulations to protect buried cable installations from damage by problem wildlife. We evaluated 2.0% mass/mass levels of capsaicin and denatonium benzoate in a polybutene carrier material (Indopol®) and an aboveground, rodent-deer plastic mesh barrier (Vexar®) for reducing gnawing by northern pocket gophers (Thomomys talpoides) on communications cable (RG-8U). When treatments were applied as surface coatings, neither capsaicin nor denatonium samples were lower (P > 0.05) in measnres of cable damage compared to control (Indopol® alone) or samples treated with Vexar® plastic mesh. When the test was repeated with a new …

Potential Associations Among Genetic Markers In The Serotonergic System And The Antisocial Alcoholism Subtype, E. M. Hill, S. F. Stoltenberg, M. Burmeister, M. Closser, R. A. Zucker

Department of Psychology: Faculty Publications

Alcoholism is transmitted in families. The complexity and heterogeneity of this disorder has made it difficult to identify specific genetic correlates. One design with the potential to do so is the family-based association study, in which the frequencies of genetic polymorphisms are compared between affected and nonaffected members. Reduced central serotonin neurotransmission is associated with features of an antisocial subtype of alcoholism, although a primary deficit has not been traced to a particular component. Genetic markers related to the serotonergic system have been identified, located, and cloned. If associations can be discovered, the development process for pharmacotherapy could be facilitated. …

Survival And Physiologic Response Of Common Amakihi And Japanese White-Eyes During Simulated Translocation, Thierry M. Work, J. Gregory Massey, Luanne Johnson, Steve Dougill, Paul C. Banko

United States Geological Survey: Staff Publications

We evaluated the effects of three translocation trials on Common Amakihi (Hemignathus virens) and Japanese White-eyes (Zosterops japonicus). Trial 1 involved capturing birds, transporting them on rough roads for 4 hr followed by holding in an aviary for 48 hr without overnight thermal support prior to release. Trial 2 involved capture, then holding in an aviary for 48 hr with overnight thermal support followed by transport for 4 hr prior to release. Trial 3 and 1 were identical except that overnight thermal support was provided during trial 3. We monitored survival, food consumption, weight change, and …

Three New Species Of Eimeria From Bolivian Marsupials, S. Kimberly Heckscher, Brian A. Wickesberg, Donald Duszynski, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Faecal samples collected from 300 Bolivian marsupials (Didelphimorphia: Didelphidae) between 1984 and 1993 were examined for coccidian parasites. Sporulated oocysts were present in the faeces of 50 (17%) marsupials representing 11 genera and 22 species. Three new species of Eimeria are described and named from six host species. One species occurred in Marmosops dorothea, Monodelphis domestica and Thylamys venustus, another in Micoureus constantiae constantiae and Micoureus constantiae budini and a third in Marmosops dorothea. A discriminant analysis performed on five quantitative oocyst measurements revealed similarities between the first and third Eimeria species because of similar sizes and …

Revisiting The Code: Clarifying Name-Bearing Types For Photomicrographs Of Protozoa, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

In 1988, Bandoni and Duszynski argued that individuals involved in the taxonomy and systematics of the coccidia should become familiar with the 3rd edition of the Code of Zoological Nomenclature (Ride et aI., 1985) and tried to reiterate the importance and utility of designating some form of type specimens and archiving them in appropriate museums. Their intent was to create an awareness among biologists working with coccidia (or other protists) of the value of designating type specimens. In the decade since their admonition, bench scholars working with coccidia, and journal editors to whom they submit their work, have begun …

Six New Eimeria Species From Vespertilionid Bats From North America, Donald W. Duszynski, Damien T. Scott, Jacqee Aragon, Ashley Leach, Travis Perry

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Twenty species of bats (Molossidae, Vespertilionidae) were collected from California, New Mexico, Oregon, South Carolina, Utah, and Baja California Norte (Mexico), and 29 of 404 (7%) animals, including Antrozous pallidus, Eptesicusfuscus, Myotis auriculus, Myotis californicus, Myotis ciliolabrum, Myotis evotis, Myotis lucifugus, Myotis thysanodes, Myotis vivesi, Myotis volans, Myotis yumanensis, and Nycticeius humeralis were infected with Eimeria spp., which represent 6 new species. Sporulated oocysts of a new species from A. pallidus are subspheroidal, 24.8 × 21.6 (22-27 × 19-24) μm with a polar granule and a large globular residuum. …

Aerodrome Bird Hazard Prevention: Case Study At John F. Kennedy International Airport, Richard A. Dolbeer

United States Department of Agriculture Wildlife Services: Staff Publications

The collision of birds with aircraft is a serious problem at John F. Kennedy International Airport (JFKIA), New York. Gulls (Larus spp.) accounted for 86% of bird strikes (an aircraft striking >1 bird) from 1988-1990, averaging 260 strikes/year. Laughing Gulls (L. atricilla) are present from May- September in association with a protected nesting colony in Jamaica Bay National Wildlife Refuge adjacent to the airport. The colony increased from 15 nests in 1979 to 7,629 nests in 1990. During the 1970s and 1980s, JFKIA implemented various management activities to reduce gull strikes, including maintenance of tall grass, improved sanitation, drainage of …

Structure, Biodiversity, And Historical Biogeography Of Nematode Faunas In Holarctic Ruminants: Morphological And Molecular Diagnoses For Teladorsagia Boreoarticus N. Sp. (Nemadota: Ostertagiinae), Dimorphic Cryptic Species In Muskoxen (Ovibos Moschatus), Eric P. Hoberg, Kirsten J. Monsen, Susan Kutz, Michael S. Blouin

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Discovery of the ostertagiine nematode Teladorsagia boreoarcticus n. sp. in muskoxen, Ovibos moschatus, from the central Canadian Arctic highlights the paucity of knowledge about the genealogical and numerical diversity of nematode faunas characteristic of artiodactyls at high latitudes across the Holarctic. Teladorsagia boreoarcticus is a dimorphic cryptic species distinguished from Teladorsagia circumcincta/Teladorsagia trifurcata in domestic sheep by a 13% divergence in the ND4 region of mitochondrial DNA, constant differences in the synlophe, and significantly longer esophageal valve, spicules, gubernaculum, and bursa. Teladorsagia boreoarcticus represents an archaic component of the North American fauna and may have a Holarctic …

Report Of The Cucurbit Working Group, James D. Mccreight, Jack Staub

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cucumber (Cucumis sativus L.) originated in India, melon (C. melo L.) and watermelon (Citrullus lanatus) in Africa, and squash, pumpkin, and gourd (Cucurbita spp.) in the Americas. Thus, cucumber, melon, and watermelon (including citron) are relatively recent introductions to the New World. Most domesticated species of Cucurbita were introduced from Mexico, Central America, and South America with the migration of native Americans centuries earlier. Wax gourd (Benincasa hispida (Thunb.) Logr.) is from Southeast Asia. Bottle gourd (Lagenaria siceraria (Molina) Stand.) is of African origin. South Asia is the probable center of …

The Biotech Century (Book Review), Philip A. Reed

STEMPS Faculty Publications

No abstract provided.

From Stockholm To Kyoto And Back To The United States: International Environmental Law's Effect On Domestic Law, Joel B. Eisen

Law Faculty Publications

In Part I, I begin my discussion of the relationship between the two legal regimes with a brief introduction to modern international environmental law to assist those whose knowledge is as limited as mine was not too long ago. I also describe how the Allen Chair Symposium and seminar series was designed to facilitate exploration of major trends in international environmental law. Part II provides a more in-depth look at the specific relationship between domestic and international environmental law. I conclude that international environmental law informs domestic law as a wellspring of law leading to domestic innovation, a complement to …

Blocked Pathways: Potential Legal Responses To Endocrine Disrupting Chemicals, Noah M. Sachs

Law Faculty Publications

Over fifty different chemicals may have endocrine disrupting effects, and these chemicals permeate our environment. We may be exposed to them through the products we use, the food we eat, the water we drink, and the air we breath. Although there is a clear need for further research into EDCs, the legal and regulatory communities should begin to consider the types of responses that may be appropriate if the scientific evidence grows stronger.

This article describes and assesses potential legal responses to EDCs, focusing on regulation and litigation, and concludes that existing legal tools will probably be inadequate to respond …

Physical Mapping Of The Bovine, Caprine And Ovine Homologues Of The Paired Box Gene Pax8, N. L. Lòpez-Corrales, T. S. Sonstegard, T. P. L. Smith

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The PAX8 gene, a member of the human paired box gene family, was mapped by FISH to chromosome 11 in cattle and goat and to the short arm of chromosome 3 in sheep. The cytogenetic position of PAX8 on BTA 11 and on its homologue OAR 3p lies in the region where the interleukin beta (IL1B) gene has been previously located, (BTA 11q22.1→q22.3 and OAR 3p25→q26 respectively; Lòpez-Corrales et al., 1998). The results indicated that PAX8 as well as interleukin beta and interleukin alpha (IL1B and IL1A) genes detected on the human chromosome segment HSA 2q13→q21 maintain a similar …

Analysis Of Human Skin Emanations By Gas Chromatography/Mass Spectrometry. 1. Thermal Desorption Of Attractants For The Yellow Fever Mosquito (Aedes Aegypti) From Handled Glass Beads, Ulrich R. Bernier, Matthew M. Booth, Richard A. Yost

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Handled glass has the ability to collect and concentrate nonaqueous human skin emanations while minimizing the collection of aqueous perspiration. Compounds originating from the skin and collected on glass have previously been found to attract the Aedes aegypti species of mosquito. Therefore, glass beads were used as the medium to collect skin emanations from humans for subsequent chemical analysis. This process consisted of a 5-15-min collection of sample on glass beads, followed by loading the beads into a gas chromatograph (GC) injector insert for subsequent desorption of the collected compounds onto the GC column. After cryofocusing by liquid nitrogen at …

The Effects Of Moulting On Muscle Fibre Characteristics Of The Yabby, Cherax Albidus, Tina Marie Lamey

Theses : Honours

In order to grow, crustaceans must shed their exoskeleton in a process known as "moulting'. Although this process is short and intermittent, it remains dominant over the life of a crustacean. Physiological changes in the period between moults (intermoult) are comparatively quiescent as opposed to the periods directly before and after the actual moult, known as premoult, and postmoult respectively (West, 1997). Moulting is associated with distinct physiological changes including the breakdown of muscle (Musgrove and Geddes, 1985). This muscle breakdown, known as atrophy is the diminution in size of the actual muscle mass and is very specific, occurring to …

Imagining Machines: Time & Image In The Shorter Poems Of William Carlos Williams, Stuart Campbell

Theses : Honours

A discussion of time and image in the shorter poems of William Carlos Williams. The particular focus is Williams' short poems published between the Great War and World War II. The relationship between the form of the poems, and Williams' theories on poetry, as expressed in Spring and All, and The Wedge is examined. In turn, Williams' theories are viewed in the context of Imagism, Objectivist poetics, and the modernist machine aesthetic. Williams' application of these theories is seen to create a poetic form that generates images in the reader's imagination in synchrony with the moment.

Evaluating Oral Histories For Restoration Ecology, Margaret Robertson

Theses : Honours

Significant ecological restoration is required in the agricultural regions of south-western Australia. Environmental history, including local knowledge based on long-term observation of the biophysical environment, can help guide this process. Scientists already use local anecdotal information because other information sources are absent or too recent, yet they are often skeptical of its veracity. This study focused on whether environmental oral histories can be evaluated for factual accuracy and their capacity to be useful in the restoration process. Some of the complexities associated with linking environmental histories with restoration ecology, and the role played by oral histories in establishing the link …

Carmoviruses (Tombusviridae), Feng Qu, Thomas Jack Morris

School of Biological Sciences: Faculty Publications

Carmovirus is one of two officially recognized genera of Tombusviridae. Members of Tombusviridae all have icosahedral virions of about 30 nm in diameter with T = 3 symmetry that consists of 180 coat protein (CP) subunits of about 38- 43 kDa and a single-stranded (ss) RNA genome ranging in size from 4.0 to 4.7 kb. Carmoviruses share recognizable sequence similarity in both the polymerase and structural genes with members of the other genus, Tombusvirus. Sequence comparisons and phylogenetic analysis of these genes support the view that the genera are distinct but closely related. In addition, the genome organization of the …