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Expression, Purification, And Characterization Of Nonstructural Protein 2 (Ns2) Of Bluetongue Virus Serotype 17, Hai Lin

Biology

The NS2 protein of bluetongue virus (BTV) serotype 17 (B17) was expressed in large scale as a Glutathione-S-Transferase (GST) fusion protein in E. coli cells. The size of the expressed NS2/GST fusion protein was about 68 kilodaltons (kDa) when analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). This is equal to the sum of the predicted size from the deduced amino acid sequence of the NS2 protein (41 kDa) and the known mass of the GST protein (27 kDa). The NS2/GST fusion protein was purified by absorption to glutathione-conjugated agarose beads (G-beads), and then analyzed on western blots by two …

Phytotoxicity Of Pseudomonas Chlororaphis Strain O6: Role Of Phenazines And Implications For Biocontrol, Elizabeth Thomas

Biology

Phenazines, the water-soluble pigments produced naturally by bacteria with distinct antimicrobial properties, have been implicated in the biological control of several plant diseases. Pseudomonas chlororaphis strain O6, obtained from the rhizosphere of wheat, is known to produce at least three different phenazines, which include phenazine-1-carboxylic acid, 2-hydroxyphenazine-1-carboxylic acid, and 2-hydroxyphenazine. A field trial conducted in the fall of 1994 to test the biocontrol properties of O6 indicated that this strain inhibited wheat seed germination, which led me to hypothesize that phenazines cause phytotoxicity. Mutants of O6 created by Tn5 lux insertion (Phz^- mutants) that did not produce the pigments …

The Development Of A Bluetongue Virus Diagnostic Assay Using Heterologously Expressed Structural Protein Vp7 And Nonstructural Protein Ns2, Mary Anne Wise

Biology

The S1 gene of bluetongue virus that encodes major inner core protein, VP7, was cloned and expressed in the Thiofusion Expression Vector System. The VP7-thioredoxin protein was then partially purified. The nonstructural protein NS2 having previously been cloned into the GST Gene Fusion Vector System was also expressed and purified as NS2-GST fusion protein. These two viral proteins were used together in an immunologic detection assay. This assay was able to distinguish between bluetongue virus infected, vaccinated and noninfected sheep. Assayed serum samples of infected sheep, cattle, bison, and goats showed detectable antibodies to both VP7 and NS2, whereas vaccinated …

Improved Culture Of Tumor-Infiltrating Lymphocytes, Roger A. Burger

Biology

Adoptive immunotherapy using in vitro expanded tumor-infiltrating lymphocytes (TIL) continues to be the subject of research and clinical trials for the treatment of cancer. However, the culture methods used to expand large numbers of TIL are limited and often ineffective. In this study using murine TIL, the level of in vitro expansion, expression of adhesion molecules and infiltration into tumors after infusion was evaluated. A comparison was made between lymphocyte culture fluid (LCF), a bovine plasma-based medium, and a bovine serum-based medium (SCM) as the culture media. Because plasma-based media lack the lymphocyte inhibitory factors associated with serum-based media, it …

A Comparison Of Deterministic And Stochastic Population Models, Clarissa Smith

Masters Theses & Specialist Projects

In this thesis, the author considers the exponential and the predator-prey population models. A comparison of the deterministic and stochastic versions of each is made. The author shows that on average a stochastic model is precisely its corresponding deterministic model. The first model considered is the exponential model. A solution is provided for both the deterministic and the stochastic versions. A predator-prey model is also analyzed. A solution of the stochastic predator-prey model is shown to be intractable. Analysis of this model is performed through numerical simulation.

The Nighthawk, 1998, Usu Eastern English Department

Nighthawk Review

An annual publication featuring student work from Utah State University Eastern Campus.

Peaks Island Star : May 1998, Vol. 18, Issue 5, Service Agencies Of The Island

Peaks Island Star, 1998

The Peaks Island Star is the newsletter of the island's service agencies and other community news

Kentucky Warbler (Vol. 74, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

The Deaf Catholic, May-June 1998

ICDA The Deaf Catholic

A newsletter published for Deaf Catholics in USA

ICDA The Deaf CatholicFinding Aid

The Effects Of Pool Size On The Microinvertebrate Populations Of Chaney Lake, An Ephemeral Wetland Located In Bowling Green, Kentucky, Shannon Gaines

Mahurin Honors College Capstone Experience/Thesis Projects

Studies concerning the microinvertebrate communities of differing pool sizes were conducted at Chaney Lake, an ephemeral, karst wetland located in Bowling Green, Kentucky. As the lake begins to dry in the early summer, smaller, isolated ponds begin to form. Several ponds of various sizes were marked for analysis. Microinvertebrate samples and physiochemical readings were taken at each of the sites and were counted in the lab. T-tests were performed on the samples to determine if the microinvertebrate densities differed between ponds that dried later in the season as opposed to those that dried earlier. Ostracoda densities of the late dry …

Evaluation Of The Success Of Adoptive Versus Passive Transfer Of Immunity Against Trypanosoma Cruzi, Karen Powell

Masters Theses & Specialist Projects

Several investigators have demonstrated an increased resistance to the protozoal parasite Trypanosoma cruzi (the causative agent of Chagas' disease) in highly susceptible C3H mice when maintained at an environmental temperature of 36°C. This increased resistance is manifested as delay in onset of parasitemia, decreased parasitemia, decreased pathology, and increased longevity with eventual clearing of the parasite from the bloodstream. In addition, this enhanced resistance has been shown to be an immunologic phenomenon and not due to the direct effects of elevated temperature on the parasite itself. To ascertain the possible contributions of humoral vs. cellular immunity to the above phenomenon, …

Approaching Fallingwater: An Ethography Of Place, Brian Gregory

Masters Theses & Specialist Projects

Fallingwater, designed by Frank Lloyd Wright, has always been more than just a house. It has also functioned as a workplace, a tourist destination, "the best all-time work of American architecture," and a cultural symbol. By talking to some of the people involved in its history and by examining "autho-ethnographic" texts found within the community, I attempt to use ethnographic methods to understand a complicated site. Nestled in the rural Appalachian foothills of southwestern Pennsylvania, Fallingwater is also isolated. It is tempting for visitors to view it as a work of art "plopped down in the middle of nowhere." And …

Measuring The Applicability Of The Seville Strategy To The Mammoth Cave Area Biosphere Reserve, Debra Kreitzer

Masters Theses & Specialist Projects

The dominant paradigms of the past decade involve the preservation of biodiversity through the use of landscape corridors and buffer zones, as well as the integration of economic growth through sustainable use. The International Biosphere Reserve (IBR) program, as defined in the Seville Strategy, encourages locations already involved in conservation to continue to protect biodiversity and foster harmonious relationships between humans and their environment through sustainable development. An excellent example of an IBR is the Mammoth Cave Area Biosphere Reserve (MCABR) in south-central Kentucky. In order to measure the significance of the implementation of the Seville Strategy in the MCABR, …

Na/K-Atpase-Mediated 86rb+ Uptake And Asymmetrical Trophectoderm Localization Of Alpha1 And Alpha3 Na/K-Atpase Isoforms During Bovine Preattachment Development., D H Betts, L C Barcroft, A J Watson

Obstetrics & Gynaecology Publications

This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-subunit polypeptides were detected by immunofluorescence to encircle the entire cell margins of each blastomere of inseminated zygotes, cleavage stage embryos, and morulae. Immunofluorescent localization of alpha1-subunit polypeptide in bovine blastocysts revealed an alpha1 immunofluorescence signal confined to the basolateral membrane margins of the trophectoderm and encircling the cell periphery of each inner cell mass (ICM) cell. In contrast, alpha …

Children As Experimental Subjects: A Review Of Ethical And Theological Issues, Mary Catherine Bolster

The Linacre Quarterly

No abstract provided.

Visualizing Uncertainty Of The Great Plains Boundary, Sonja Lynn Rossum

Department of Geography: Dissertations, Theses, and Student Research

Geographic regions all have some degree of uncertainty, though this is seldom represented on their maps. The Great Plains is an excellent example of how even a well known and widely accepted region can be a source of cartographic confusion. This region is fuzzy in nature, with few landforms or sharp changes in characteristics to help locate the boundary line. Also, the act ofregionalization itself is a highly subjective process, using defining criteria and methods of mapping selected by the cartographer.

The uncertainty of the Great Plains boundary location is shown by comparing many such interpretations from individual cartographers, all …

Technology And Terrorism: The New Threat For The Millennium, Stephen R. Bowers, Kimberley R. Keys

Faculty Publications and Presentations

No abstract provided.

Richmond Music Journal (1998-05)

Richmond Music Journal

No abstract provided.

Clemson Commencement Program, May 1998, Clemson University

Clemson Commencement Programs

No abstract provided.

The Murray State News, May 1, 1998, The Murray State News

Murray State University Collection

No abstract provided.

Nebline, May 1998

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Teens for Teens
Perennials with colorful foliage
Watering newly planted shade trees
Lilacs in the landscape
Repotting houseplants
How harmful are high copper levels in water?
Thank you!
Upcoming household hazardous waste collections
Tips for attracting birds
Yes, it’s safe, but does it work?
Snakes in the yard
Timing window approaching for control of leafy spurge
Soybean seed treatment good idea in cool, moist soils
Proper ballast improves tractor efficiency, stability
Controlling bolting musk thistle
Managing ponds for fish
Weed control in new tree plantings
Milk calcium for all it’s worth!
Focus on Food
The Clean Hands Campaign
How clean …

Reporter - May 1st 1998, Rit Reporter Staff

University Publications

No abstract provided.

The New Hampshire, Vol. 88, No. 49 (May 1, 1998), The New Hampshire

The New Hampshire Print Edition

An independent student produced newspaper from the University of New Hampshire.

The Bison, May 1, 1998

The Bison Newspapers

No abstract provided.

The Problematics Of Moral And Legal Theory, Richard A. Posner

Articles

In these Holmes Lectures, delivered a century after the publication of Oliver Wendell Holmes's great essay The Path of the Law, Judge Posner argues for an essentially Holmesian conception of the proper relations among modern normative moral philosophy ("academic moralism"), morality, and law. Academic moralism, he argues, lacks either the intellectual cogency or the emotional power to change people's beliefs or behavior; the power to do so resides in "moral entrepreneurs," which academic moralists emphatically are not. Academic moralism's lack of cogency disqualifies it to guide judicial decisionmaking even - in fact, especially - in cases involving controversial moral issues, …

The Reproductive Biology And Ecology Of Penstemon Harringtonii (Scrophulariacea): Implications For Conservation, Romaine L. Nielson

Biology

I examined the reproductive biology of Harrington's Penstemon, a rare endemic of the Colorado Plateau, to obtain information important to conservation efforts for this species. I applied four different hand-pollination treatments to P. harringtonii's flowers and found that it is partially self-compatible but sets more fruits when cross-pollinated by insects. Plant placement in new populations may be important because pollinators are more attracted to dense floral resources. Thus, the fruit set of plants within a 450 cm² area was compared with those at least 3 m apart. Unexpectedly, aggregated plants produced 16.1 fruits per plant compared to 21.0 …

Factors Concerning The Conservation Of A Rare Shale Endemic Plant: The Reproductive Ecology And Edaphic Characteristics Of Penstemon Debilis (Scrophulariaceae), Amy L. Mcmullen

Biology

The management of a rare, edaphic endemic plant species requires an understanding of the ecological setting and processes that contribute to its restriction. Included in the ecological processes is a set of biotic characters associated with the reproductive ecology, including its breeding system, pollination vectors, and germination requirements. The geologic and soil setting of the plant must also be characterized in order to understand the plant's restriction to the substrate. These issues were addressed by this study of the rare endemic Penstemon debilis O'Kane and Anderson (Scrophulariaceae). Penstemon debilis consists of six populations that occur on talus slopes of the …

Phylogenetic Characterization Of Endosymbionts In Psyllids (Hemiptera: Sternorrhyncha), Allen W. Spaulding

Biology

Most sternorrhynchan insects harbor endosymbiotic bacteria in specialized cells (bacteriocytes) near the gut, which provide essential nutrients for hosts. In lineages investigated so far with molecular methods (aphids, mealybugs, whiteflies), endosymbionts apparently have arisen from independent infections of common host ancestors and cospeciated with their hosts. Some endosymbionts also exhibit putatively negative genetic effects from their symbiotic association. In this study, the identity of endosymbionts in one major sternorrhynchan lineage, psyllids (Psylloidea), was investigated to determine their position in eubacterial phylogeny and relationship to other sternorrhynchan endosymbionts. Small-subunit ribosomal RNA genes (16S rDNA) from bacteria in three psyllid species (families …

The Reproductive Ecology, Demography, And Population Genetic Structure Of Arctomecon Californica Torrey & Fremont (Papaveraceae) In Fragmented And Unfragmented Habitat, Laura L. Hickerson

Biology

Arctomecon californica (the Las Vegas bearpoppy) is endemic to gypsum outcrops of the northern Mojave Desert. Native habitat of this plant in the Las Vegas Valley has been severely fragmented, while relatively undisturbed, unfragmented habitat still exists in the Lake Mead National Recreation Area. I studied the reproductive ecology and population genetic structure of this rare plant. Comparisons were then drawn between fragmented and unfragmented habitat. Arctomecon californica flowers are mostly self-incompatible, and require a pollen vector for successful seed production. Evidence of pollinator limitation was found in both habitat types, but plants in fragmented habitat were much less successful …

An Analysis Of Osa Johnson - Noted Female Explorer, Frederick Michael Mccreedy

Electronic Theses & Dissertations

"An Analysis of Osa Johnson Noted Female Explorer" examines the life of the wife of famed African explorer Martin Elmer Johnson. It is the story of a love affair between two people who also shared a mutual love of animals and nature. Osa was initially the concerned wife who looked out after her husband and attempted to safeguard their lives in the early years of this century in a continent fraught with dangerous carnivores and instant death at every imaginable corner. She progressed from this to a position of leadership, shared with her husband, as they truly became a team …