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Evolution Of Puma Lentivirus In Bobcats (Lynx Rufus) And Mountain Lions (Puma Concolor) In North America, Justin S. Lee, Sarah N. Bevins, Laurel E. K. Serleys, Winston Vickers, Ken A. Logan, Mat Aldredge, Erin E. Boydston, Lisa M. Lyren, Roy Mcbride, Melody Roelke-Parker, Jill Pecon-Slattery, Jennifer L. Troyer, Seth P. Riley, Walter M. Boyce, Kevin R. Crooks, Sue Vandewoude

United States Department of Agriculture Wildlife Services: Staff Publications

Mountain lions (Puma concolor) throughout North and South America are infected with puma lentivirus clade B (PLVB). A second, highly divergent lentiviral clade, PLVA, infects mountain lions in southern California and Florida. Bobcats (Lynx rufus) in these two geographic regions are also infected with PLVA, and to date, this is the only strain of lentivirus identified in bobcats. We sequenced full-length PLV genomes in order to characterize the molecular evolution of PLV in bobcats and mountain lions. Low sequence homology (88% average pairwise identity) and frequent recombination (1 recombination breakpoint per 3 isolates analyzed) were observed …

Short Report: Antibody Prevalence Of Select Arboviruses In Mute Swans (Cygnus Olor) In The Great Lakes Region And Atlantic Coast Of The United States, Kerri Pedersen, David R. Marks, Dustin M. Arsnoe, Sarah N. Bevins, Eryu Wang, Scott C. Weaver, Randall M. Mickley, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

Mute swans (Cygnus olor) are an invasive species in the United States. The dramatic increase in their populations in localized areas has led to various problems, among them competition with native species and attacks on humans by aggressive swans. However, very little is known about the ability of these swans to transmit pathogens to humans, domestic birds, or wildlife or participate in enzootic maintenance. To learn more about select pathogens that mute swans may harbor, a survey was conducted from April of 2011 to August of 2012 in the Great Lakes region and localized areas of the Atlantic …

Right Place, Wrong Species: A 20-Year Review Of Rabies Virus Cross Species Transmission Among Terrestrial Mammals In The United States, Ryan M. Wallace, Amy T. Gilbert, Dennis Slate, Richard Chipman, Amber Singh, Cassie Wedd, Jesse D. Blanton

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction: In the continental US, four terrestrial mammalian species are reservoirs for seven antigenic rabies virus variants. Cross species transmission (CST) occurs when a rabies virus variant causes disease in non-reservoir species.

Methods: This study analyzed national surveillance data for rabies in terrestrial mammals. The CST rate was defined as: number of rabid non-reservoir animals/number of rabid reservoir animals. CST rates were analyzed for trend. Clusters of high CST rate counties were evaluated using space-time scanning statistics.

Results: The number of counties reporting a raccoon variant CST rate >1.0 increased from 75 in 1992 to 187 in 2011; counties with …

Avian Paramyxovirus Serotype 1 (Newcastle Disease Virus), Avian Influenza Virus, And Salmonella Spp. In Mute Swans (Cygnus Olor) In The Great Lakes Region And Atlantic Coast Of The United States, Kerri Pedersen, David R. Marks, Dustin M. Arsnoe, Claudio L. Afonso, Sarah N. Bevins, Patti J. Miller, Adam R. Randall, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

Since their introduction to the United States in the late 19th century, mute swans (Cygnus olor) have become a nuisance species by causing damage to aquatic habitats, acting aggressively toward humans, competing with native waterfowl, and potentially transmitting or serving as a reservoir of infectious diseases to humans and poultry. In an effort to investigate their potential role as a disease reservoir and to establish avian health baselines for pathogens that threaten agricultural species or human health, we collected samples from 858 mute swans and tested them for avian paramyxovirus serotype 1 (APMV-1), avian influenza virus (AIV), and Salmonella spp. …

Characteristics Of White-Tailed Deer Visits To Cattle Farms: Implications For Disease Transmission At The Wildlife-Livestock Interface, Are R. Berentsen, Ryan S. Miller, Jennifer L. Malmberg, Mike R. Dunbar

United States Department of Agriculture Wildlife Services: Staff Publications

Bovine tuberculosis (bTB) is endemic in free-ranging white-tailed deer (Odocoileus virginianus) in MI, USA. Currently, the rates of farm visitation by deer and co-use of forage resources by cattle and deer are poorly understood. To evaluate the extent deer and livestock may share forage resources, we investigated farm, yard, and cattle-use area visitation by white-tailed deer and compared visitation with common livestock management practices. We fitted 25 female white-tailed deer near the bTB-infected zone in Michigan's Lower Peninsula with global positioning system collars. Livestock management practices associated with farm visitation included presence of confined feeding pastures, number of cattle water …

Crossing The Interspecies Barrier: Opening The Door To Zoonotic Pathogens, Christian Gortazar, Leslie A. Reperant, Thijs Kuiken, Jose De La Fuente, Mariana Boadella, Beatriz Martinez-Lopez, Francisco Ruiz-Fons, Agusitn Estrada-Pena, Christian Drosten, Graham Medley, Richard Ostfeld, Townsend Peterson, Kurt C. Vercauteren, Christian Menge, Marc Artois, Constance Schultsz, Richard Delahay, Jordi Sierra-Cobo, Robert Poulin, Frederic Keck, Alonso A. Aguirre, Heikki Henttonen, Andrew P. Dobson, Susan Kutz, Juan Lubroth, Atle Mysterud

United States Department of Agriculture Wildlife Services: Staff Publications

The number of pathogens known to infect humans is ever increasing. Whether such increase reflects improved surveillance and detection or actual emergence of novel pathogens is unclear. Nonetheless, infectious diseases are the second leading cause of human mortality and disability-adjusted life years lost worldwide [1,2]. On average, three to four new pathogen species are detected in the human population every year [3]. Most of these emerging pathogens originate from nonhuman animal species. Zoonotic pathogens represent approximately 60% of all known pathogens able to infect humans [4]. Their occurrence in humans relies on the human-animal interface, defined as the continuum of …

Diffusion Of Influenza Viruses Among Migratory Birds With A Focus On The Southwest United States, Matthew Scotch, Tommy Tsan-Yuk Lam, Kristy L. Pabilonia, Theodore Anderson, John Baroch, Dennis Kohler, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

The Southwest United States, including Arizona and New Mexico, has a diverse climate and is home to many different avian species. We sequenced the hemagglutinin (HA) gene of twenty influenza specimens for the years 2007–2009. This included four from Arizona, and sixteen from New Mexico. We analyzed the sequences and determined the following HA subtypes: H3, H4, H6, H8, and H11. For each subtype, we combined our virus sequences with those from a public database, and inferred phylogeographic models of influenza diffusion. Statistical phylogeography indicated that overall evolutionary diffusion of avian influenza viruses is geographically structured (p < 0.05). In addition, we found that diffusion to the Southwest was often from nearby states including California. For H3, H4 and H6, the intra-flyway gene flow rates were significantly (p < 0.001) higher than those of inter-flyway. Such rate difference was also observed in H8 and H11, yet, without statistical significance (p = 0.132, p = 0.190, respectively). Excluding any one flyway from the calculation generated similar results, suggesting that such barrier effect on gene flow rates is not exclusively produced by any single flyway. We also calculated the Bayes factor test for the significant non-zero rates between states and identified significant routes both within and across flyways. Such inter-flyway spread of influenza was probably the result of birds from four flyways co-mingling on breeding grounds in northern regions or marshaling on staging areas post breeding in Canada or Alaska, before moving south each fall. This study provides an initial analysis of evolutionary diffusion of avian influenza virus to and from the Southwest United States. However, more sequences from this region need to be generated to determine the role of host migration and other factors on influenza diffusion.

Exposure Of Feral Swine (Sus Scrofa) In The United States To Selected Pathogens, John A. Baroch, Carl A. Gagnon, Sonia Lacouture, Marcelo Gottschalk

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine (Sus scrofa) are widely distributed in the United States. In 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the US to evaluate exposure to important swine endemic pathogens. Antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) were found in 2.5% and 25.3% of tested sera, respectively. Positive serological reactions against Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae have been detected in 19.7% and 69.7% of animals. More than 15% of animals presented antibodies against these 2 pathogens simultaneously. Most animals were also seropositive for …

Risk Factors Associated With Enteric Septicemia Of Catfish On Mississippi Commercial Catfish Farms, Fred L. Cunningham, S. W. Jack, David Hardin, Robert W. Wills

United States Department of Agriculture Wildlife Services: Staff Publications

A gram-negative bacterium, Edwardsiella ictaluri, is the cause of enteric septicemia of catfish (ESC), which is one of the most prevalent bacterial diseases in farm-raised catfish. The objective of this study was to identify risk factors associated with ESC mortalities and are reported by farm personnel. To identify risk factors a catfish management database was developed. The odds ratios (OR) of the final multivariable logistic regression model were: (1) volume of the pond (OR, 0.56), (2) interval from harvest until a mortality event (OR, 1.49), (3) interval from stocking until a mortality event (OR, 0.52), (4) nitrite measured within 14 …

Mineral Licks: Motivational Factors For Visitation And Accompanying Disease Risk At Communal Use Sites Of Elk And Deer, Michael J. Lavelle, Gregory E. Phillips, Justin W. Fischer, Patrick W. Burke, Nathan W. Seward, Randal S. Stahl, Tracy A. Nichols, Bruce A. Wunder, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Free-ranging cervids acquire most of their essential minerals through forage consumption, though occasionally seek other sources to account for seasonal mineral deficiencies. Mineral sources occur as natural geological deposits (i.e., licks) or as anthropogenic mineral supplements. In both scenarios, these sources commonly serve as focal sites for visitation. We monitored 11 licks in Rocky Mountain National Park, north-central Colorado, using trail cameras to quantify daily visitation indices (DVI) and soil consumption indices (SCI) for Rocky Mountain elk (Cervus elaphus) and mule deer (Odocoileus hemionus) during summer 2006 and documented elk, mule deer, and moose (Alces …

First Reports Of Pseudorabies And Winter Ticks (Dermacentor Albipictus) Associated With An Emerging Feral Swine (Sus Scrofa) Population In New Hampshire, Anthony R. Musante, Kerri Pedersen, Parker Hall

United States Department of Agriculture Wildlife Services: Staff Publications

The expansion of feral swine (Sus scrofa) populations into new geographic regions is of concern not only due to increased range but also because they carry diseases and parasites that pose a threat to humans, livestock, and wildlife into new areas. Recently, emerging feral swine populations have been reported in the northeastern US and due to their adaptive nature will likely continue to spread. During 2009–2012, 49 feral swine were removed from three counties in New Hampshire. Of these, serum samples were submitted from 34 for disease surveillance testing. One of the feral swine was antibody-positive for pseudorabies virus (PRV) …

Using Quantitative Disease Dynamics As A Tool For Guiding Response To Avian Influenza In Poultry In The United States Of America, K. M. Pepin, E. Spackman, J. D. Brown, K. L. Pabilonia, Lindsey P. Garber, J. Todd Weaver, D. A. Kennedy, Kelly A. Patyk, K. P. Huyvaert, Ryan S. Miller, Alan B. Franklin, Kerri Pedersen, T. L. Bogich, P. Rohani, Susan A. Shriner, Colleen T. Webb, S. Riley

United States Department of Agriculture Wildlife Services: Staff Publications

Wild birds are the primary source of genetic diversity for influenza A viruses that eventually emerge in poultry and humans. Much progress has been made in the descriptive ecology of avian influenza viruses (AIVs), but contributions are less evident from quantitative studies (e.g., those including disease dynamic models). Transmission between host species, individuals and flocks has not been measured with sufficient accuracy to allow robust quantitative evaluation of alternate control protocols. We focused on the United States of America (USA) as a case study for determining the state of our quantitative knowledge of potential AIV emergence processes from wild hosts …

A Conceptual Model For The Impact Of Climate Change On Fox Rabies In Alaska, 1980–2010, B. I. Kim, J. D. Blanton, A. T. Gilbert, L. Castrodale, K. Hueffer, D. Slate, C. E. Rupprecht

United States Department of Agriculture Wildlife Services: Staff Publications

The direct and interactive effects of climate change on host species and infectious disease dynamics are likely to initially manifest at latitudinal extremes. As such, Alaska represents a region in the United States for introspection on climate change and disease. Rabies is enzootic among arctic foxes (Vulpes lagopus) throughout the northern polar region. In Alaska, arctic and red foxes (Vulpes vulpes) are reservoirs for rabies, with most domestic animal and wildlife cases reported from northern and western coastal Alaska. Based on passive surveillance, a pronounced seasonal trend in rabid foxes occurs in Alaska, with a peak in winter and spring. …

Bat Rabies In Guatemala, James E. Ellison, Amy T. Gilbert, Sergio Recuenco, David Moran, Danilo A. Alvarez, Natalia Kuzmina, Daniel L. Garcia, Leonard F. Peruski, Mary T. Mendonca, Kim A. Lindblade, Charles E. Rupprecht

United States Department of Agriculture Wildlife Services: Staff Publications

Rabies in bats is considered enzootic throughout the New World, but few comparative data are available for most countries in the region. As part of a larger pathogen detection program, enhanced bat rabies surveillance was conducted in Guatemala, between 2009 and 2011. A total of 672 bats of 31 species were sampled and tested for rabies. The prevalence of rabies virus (RABV) detection among all collected bats was low (0.3%). Viral antigens were detected and infectious virus was isolated from the brains of two common vampire bats (Desmodus rotundus). RABV was also isolated from oral swabs, lungs and …

Shedding Of A Low Pathogenic Avian Influenza Virus In A Common Synanthropic Mammal – The Cottontail Rabbit, J. Jeffrey Root, Susan A. Shriner, Kevin T. Bentler, Thomas Gidlewski, Nicole L. Mooers, Terry R. Spraker, Kaci K. Vandalen, Heather J. Sullivan, Alan B. Franklin

United States Department of Agriculture Wildlife Services: Staff Publications

Background: Cottontails (Sylvilagus spp.) are common mammals throughout much of the U.S. and are often found in peridomestic settings, potentially interacting with livestock and poultry operations. If these animals are susceptible to avian influenza virus (AIV) infections and shed the virus in sufficient quantities they may pose a risk for movement of avian influenza viruses between wildlife and domestic animals in certain situations.

Methodology/Principal Findings: To assess the viral shedding potential of AIV in cottontails, we nasally inoculated fourteen cottontails with a low pathogenic AIV (H4N6). All inoculated cottontails shed relatively large quantities of viral RNA both nasally ( …

Ecological Routes Of Avian Influenza Virus Transmission To A Common Mesopredator: An Experimental Evaluation Of Alternatives, J. Jeffrey Root, Kevin T. Bentler, Susan A. Shriner, Nicole L. Mooers, Kaci K. Vandalen, Heather J. Sullivan, Alan B. Franklin

United States Department of Agriculture Wildlife Services: Staff Publications

Background: Wild raccoons have been shown to be naturally exposed to avian influenza viruses (AIV). However, the mechanisms associated with these natural exposures are not well-understood.

Methodology/Principal Findings: We experimentally tested three alternative routes (water, eggs, and scavenged waterfowl carcasses) of AIV transmission that may explain how raccoons in the wild are exposed to AIV. Raccoons were exposed to 1) water and 2) eggs spiked with an AIV (H4N6), as well as 3) mallard carcasses experimentally inoculated with the same virus. Three of four raccoons exposed to the high dose water treatment yielded apparent nasal shedding of >10^2.0 PCR …

Spatial Epidemiology Of Escherichia Coli O157:H7 In Dairy Cattle In Relation To Night Roosts Of Sturnus Vulgaris (European Starling) In Ohio, Usa (2007–2009), A. L. Swirski, D. L. Pearl, M. L. Williams, H. J. Homan, G. M. Linz, N. Cernicchiaro, J. T. Lejeune

United States Department of Agriculture Wildlife Services: Staff Publications

The goal of our study was to use spatial scan statics to determine whether the night roosts of European starlings (Sturnus vulgaris) act as point sources for the dissemination of Escherichia coli O157:H7 among dairy farms. From 2007 to 2009, we collected bovine faecal samples (n = 9000) and starling gastrointestinal contents (n = 430) from 150 dairy farms in northeastern Ohio, USA. Isolates of E. coli O157:H7 recovered from these samples were subtyped using multilocus variable-number tandem repeat analysis (MLVA). Generated MLVA types were used to construct a dendrogram based on a categorical multistate coefficient and unweighted …

Genetic Analysis Of A Bison Bison Herd Derived From The Yellowstone National Park Population, Julia A. Herman, Antoinette J. Piaggio, Natalie D. Halbert, Jack C. Rhyan, M. D. Salman

United States Department of Agriculture Wildlife Services: Staff Publications

The objective of this study is to inventory the current genetic diversity of the bison quarantine feasibility study (BQFS) herd originating from Yellowstone National Park (YNP) using previously described microsatellite, mitochondrial and nuclear DNA markers with the aim to determine the degree, if any, of cattle DNA introgression in this herd. This work can provide an important tool in monitoring and managing bison genetic diversity as brucellosis-free reintroduced herds are re-established throughout the US for conservation purposes. The BQFS composed of 89 Bison bison from YNP that were quarantined and tested to qualify as free of brucellosis in 2006 – …

Environmental Salmonella In Agricultural Fair Poultry Exhibits In Colorado, K. L. Pabilonia, K. J. Cadmus, T. M. Lingus, D. S. Bolte, M. M. Russell, D. C. Van Metre, M. M. Erdman

United States Department of Agriculture Wildlife Services: Staff Publications

Salmonella enterica is a common zoonotic pathogen in humans. Transmission typically occurs through consumption of contaminated food products or contact with infected animals, including poultry or their environment. The objective of this study was to estimate the frequency of Salmonella contamination in the environment in poultry exhibits at agricultural fairs. Samples were collected from cages, feed, floors and tables in the exhibit and cultured for Salmonella. At least one environmental sample was positive for Salmonella in 10 of 11 fairs (91%), and Salmonella was isolated from 28 of 55 environmental samples (50.9%). Eleven different serotypes were detected. Results of …

Predicted Wildlife Disease-Related Climate Change Impacts Of Specific Concern To Usda Aphis Wildlife Services, Timothy P. Algeo, Richard B. Chipman, Dennis Slate, Jerome E. Freier, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

USDA APHISWildlife Services plans for and responds to a variety of exigencies such as wildlife hazards to aircraft, disease emergence from wildlife translocations, oral rabies vaccine barrier compromises, and extreme weather events. These are often collaborative efforts with state and federal agencies and others. Climate change based in part on fossil fuel use and methane gas emissions has predictable as well as unknown consequences. As a federal leader in wildlife disease research and management, it is incumbent upon Wildlife Services to be current with the scientific literature; assess potential impacts and wildlife disease management intervention needs from predicted climate change …

Wildlife Damage Management: Changes Over The Last 40 Years And A Look At The Future, Kathleen A. Fagerstone

United States Department of Agriculture Wildlife Services: Staff Publications

Since becoming a wildlife biologist 40 years ago, I have seen many changes. Yet some things have remained the same, like the economic impact of wildlife damage, which was high in 1974 and even higher now. In 2014, the worldwide cost of damage by vertebrate pests to agriculture will exceed $1 billion. The world’s human population has increased at an unprecedented rate, while some wildlife populations have also burgeoned over the past 40 years due to land-use changes and effective management programs. These simultaneous human and wildlife population increases have led to increasing conflicts between humans and wildlife. Nor has …

A Recent Epizootic Of Skunk Rabies And Associated Spillover In Northern Colorado, Usa, Amy Gilbert, Dennis Kohler, Tara Rigg, Justin W. Fischer, Terry Spraker, Karen Fox, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Rabies is a fatal viral zoonosis that is transmitted by bite contact with saliva of an infected animal. In the U.S., human deaths are rare, but each year over 5,000 rabid animals are reported and over 90% of cases are from wildlife. Multiple species of carnivores and bats are the primary sylvatic reservoirs in the U.S., and several variants circulate independently among these two taxonomic orders. Rabies prevention efforts led by USDA APHIS Wildlife Services using live recombinant vaccine baiting began in 1995 and currently target raccoons and canids, but the territory of the south-central skunk rabies virus variant has …

Fitting Outbreak Models To Data From Many Small Norovirus Outbreaks, Eamon B. O'Dea, Kim M. Pepin, Ben A. Lopman, Claus O. Wilke

United States Department of Agriculture Wildlife Services: Staff Publications

Infectious disease often occurs in small, independent outbreaks in populations with varying characteristics. Each outbreak by itself may provide too little information for accurate estimation of epidemic model parameters. Here we show that using standard stochastic epidemic models for each outbreak and allowing parameters to vary between outbreaks according to a linear predictor leads to a generalized linear model that accurately estimates parameters from many small and diverse outbreaks. By estimating initial growth rates in addition to transmission rates, we are able to characterize variation in numbers of initially susceptible individuals or contact patterns between outbreaks. With simulation, we find …

An Agent-Based Movement Model To Assess The Impact Of Landscape Fragmentation On Disease Transmission, Jeff A. Tracey, Sarah N. Bevins, Sue Vandewoude, Kevin R. Crooks

United States Department of Agriculture Wildlife Services: Staff Publications

Landscape changes can result in habitat fragmentation and reduced landscape connectivity, limiting the ability of animals to move across space and altering infectious disease dynamics in wildlife. In this study, we develop and implement an agent-based model to assess the impacts of animal movement behavior and landscape structure on disease dynamics. We model a susceptible/infective disease state system applicable to the transmission of feline immunodeficiency virus in bobcats in the urbanized landscape of coastal southern California. Our agent-based model incorporates animal movement behavior, pathogen prevalence, transmission probability, and habitat fragmentation to evaluate how these variables influence disease spread in urbanizing …

Detection Of Volatile Organic Compounds In Brucella Abortus- Seropositive Bison, Alona Bayn, Pauline Nol, Ulrike Tisch, Jack Rhyan, Christine K. Ellis, Hossam Haick

United States Department of Agriculture Wildlife Services: Staff Publications

Brucellosis is of great public health and economic importance worldwide. Detection of brucellosis currently relies on serologic testing of an antibody response to Brucella infection, which suffers from cross-sensitivities to other antibody responses. Here we present a new method for identifying Brucella exposure that is based on profiling volatile organic compounds (VOCs) in exhaled breath. Breath samples from Brucella-seropositive bison and controls were chemically analyzed and demonstrated statistically significant differences in the concentration profiles of five VOCs. A point-of-care device incorporating an array of nanomaterial-based sensors could identify VOC patterns indicative of Brucella exposure with excellent discriminative power, using …

Deciphering Serology To Understand The Ecology Of Infectious Diseases In Wildlife, Amy T. Gilbert, A. R. Fooks, D. T. S. Hayman, D. L. Horton, T. Muller, R. Plowright, A. J. Peel, R. Bowen, J. L. Wood, J. Miils, A. A. Cunningham, C. E. Rupprecht

United States Department of Agriculture Wildlife Services: Staff Publications

The ecology of infectious disease in wildlife has become a pivotal theme in animal and public health. Studies of infectious disease ecology rely on robust surveillance of pathogens in reservoir hosts, often based on serology, which is the detection of specific antibodies in the blood and is used to infer infection history. However, serological data can be inaccurate for inference to infection history for a variety of reasons. Two major aspects in any serological test can substantially impact results and interpretation of antibody prevalence data: cross-reactivity and cut-off thresholds used to discriminate positive and negative reactions. Given the ubiquitous use …

Nwrc Annual Publications List – 2013

United States Department of Agriculture Wildlife Services: Staff Publications

No abstract provided.

New World Bats Harbor Diverse Influenza A Viruses, Suxiang Tong, Xueyong Zhu, Yan Li, Mang Shi, Jing Zhang, Melissa Bourgeois, Hua Yang, Xianfeng Chen, Sergio Recuenco, Jorge Gomez, Li-Mei Chen, Adam Johnson, Ying Tao, Cyrille Dreyfus, Wenli Yu, Ryan Mcbride, Paul J. Carney, Amy T. Gilbert, Jessie Chang, Zhu Guo, Charles T. Davis, James C. Paulson, James Stevens, Charles E. Rupprecht, Edward C. Holmes, Ian A. Wilson, Ruben O. Donis

United States Department of Agriculture Wildlife Services: Staff Publications

Aquatic birds harbor diverse influenza A viruses and are a major viral reservoir in nature. The recent discovery of influenza viruses of a new H17N10 subtype in Central American fruit bats suggests that other New World species may similarly carry divergent influenza viruses. Using consensus degenerate RT-PCR, we identified a novel influenza A virus, designated as H18N11, in a flat-faced fruit bat (Artibeus planirostris) from Peru. Serologic studies with the recombinant H18 protein indicated that several Peruvian bat species were infected by this virus. Phylogenetic analyses demonstrate that, in some gene segments, New World bats harbor more influenza …

Spatial Ecology Of Urban Raccoons In Northeastern Ohio: Implications For Oral Rabies Vaccination, Are R. Berentsen, Mike R. Dunbar, Chadd E. Fitzpatrick, W. David Walter

United States Department of Agriculture Wildlife Services: Staff Publications

In 1977, rabies was detected in a raccoon (Procyon lotor) in West Virginia, and since the mid-1980s raccoon variant rabies has spread throughout the eastern United States and moved west as far as the eastern edge of Cleveland, Ohio. The primary tool to combat this spread is the distribution of oral rabies vaccine (ORV) baits. A thorough knowledge of raccoon space use is critical in determining bait placement, particularly in urban areas. We monitored nine raccoons in urban areas of Cleveland, Ohio, calculated home range sizes, monitored raccoon movement with respect to potential movement barriers, and used resource …

Surveillance And Movements Of Virginia Opossum (Didelphis Virginiana) In The Bovine Tuberculosis Region Of Michigan, W. D. Walter, J. W. Fischer, C. W. Anderson, D. R. Marks, T. J. Deliberto, S. Robbe-Austerman, K. C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife reservoir hosts of bovine tuberculosis (bTB) include Eurasian badgers (Meles meles) and brushtail possum (Trichosurus vulpecula) in the UK and New Zealand, respectively. Similar species warrant further investigation in the northern lower peninsula of Michigan, USA due to the continued presence of bTB on cattle farms. Most research in Michigan, USA has focused on interactions between white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus) for the transmission of the infectious agent of bTB, Mycobacterium bovis, due to high deer densities and feeding practices. However, limited data are available on medium-sized mammals …