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Transcriptome Studies On The Toxicity Of Silica Nanoparticles, Shih-Yi Hsu

USF Tampa Graduate Theses and Dissertations

Nanotechnology enables more precise harmony in health condition via reducing the dosage amount, improving the delivery of hydrophobic drugs, more specific targeting to the cancerous sites, and so on. Nevertheless, issues regarding the toxicity of nanotechnology have begun to call for attention several decades later after the innovation of nanotechnology. Tools about risk management of nanotechnology have been developed, but recently not much evidence recognizes the toxicity of nanoparticles (NPs) except for some animal studies, which demonstrated organ damage after the exposure to NPs. Toxicogenomic approach refers to the method utilizing gene expression to evaluate the chemical toxicity. Databases as …

Assessing The Impacts Of Sensorimotor Stimuli And Nicotine Content On Cravings And Other Outcomes Of E-Cigarette Use, Amanda M. Palmer

USF Tampa Graduate Theses and Dissertations

As use rates of e-cigarettes continue to rise, especially among cigarette smokers, there remains concern that “dual use” may lead to increased dependence and hinder smoking cessation efforts. At the same time, emerging evidence suggests clinical efficacy of e-cigarettes. The role of nicotine must be considered, in addition to non-pharmacologic influences, such as expectancies or conditioned reinforcers. Sensorimotor stimuli associated with drug delivery have been demonstrated to produce cigarette and e-cigarette craving reduction, even without the nicotine. The purpose of the present study was to parse the influences of nicotine and sensorimotor delivery on various outcomes of e-cigarette use, including …

Cancer Biomarker Detection In Human Plasma By Integrating Surface Acoustic Wave And Metal-Enhanced Fluorescence, Yuqi Huang

USF Tampa Graduate Theses and Dissertations

This thesis includes data and discussion about the technique of metal-enhanced fluorescence (MEF) to lower the detection limit of carcinoembryonic antigen (CEA). The detection limit goes down to 100pg/mL level when using MEF substrate made by rapid thermally annealed silver film covered by silica, which has great promise in diagnosing certain types of cancer that uses CEA as detection biomarker, such as pancreatic cancer and colon cancer. To further address the issue of background noises from non-specifically bound proteins (NSB) in complex media, such as plasma, serum, urine and blood, MEF is integrated with surface acoustic wave (SAW) streaming in …

Exploring The Potential Of Nutrient Retention And Recycling With Wetlaculture Systems In Ohio With Physical And Landscape Models, Bingbing Jiang

USF Tampa Graduate Theses and Dissertations

Significant expansion of agricultural land use has been widely recognized for leading to global and regional negative environmental impacts, especially increased eutrophication of surface water systems for the last half-century. The landscape-scale environmental problem of overloading nutrients to lakes and streams by excessive fertilizer use and increased human-caused N-fixation is in urgent need of a sustainable landscape-scale solution. Wetlands have long been considered as an effective way to remove nutrients from surface water. However, the influence of regional seasonality and hydrologic conditions on agricultural runoff treatment wetlands still needs more investigation. A new approach, “wetlaculture,” has recently described as a …

Balance Function: A Potential Early Indicator Of Mild Cognitive Impairment, Karen L. Bell

USF Tampa Graduate Theses and Dissertations

This study examined oculomotor and vestibular function in young adults, cognitively normal older adults (CNOA), and older adults with MCI. Oculomotor and vestibular function were assessed using videonystagmography (VNG), vestibular evoked myogenic potentials (VEMPs), the modified Clinical Test of Sensory Integration and Balance (mCTSIB), and the Fukuda Stepping test. Forty-two participants comprised three groups (young adults, n = 15; CNOA, n = 13; MCI, n = 14). The Montreal Cognitive Assessment ([MoCA]; Nasreddine et al., 2005) was used to assess cognitive status, and a MCI diagnosis was confirmed using standardized neuropsychological testing and a physician’s exam. MANOVA was calculated to …

Non-Invasive Sex Determination And Genotyping Of Transgenic Brugia Malayi Larvae, Santiago E. Hernandez Bojorge

USF Tampa Graduate Theses and Dissertations

Lymphatic filariasis is a very painful and disfiguring helminth disease caused by the tissue nematodes Wuchereria bancrofti, Brugia malayi, and Brugia timori. This parasitosis is considered a Neglected Tropical Disease and it is a major public health burden for 72 tropical countries of Africa, Southeast Asia, the Caribbean, and South America. Despite the effectiveness of many control programs, there remains the need to develop new pharmacological agents to treat lymphatic filariasis, as most programs rely on a limited variety of drugs that are expensive, logistically difficult to obtain, and can lead to drug resistance. The Clustered Regularly Interspaced Short Palindromic …

Predictors Of Nonadherence To Radiation Therapy Schedules Among Head And Neck Cancer Patients, Jennifer Lynn Miller

USF Tampa Graduate Theses and Dissertations

Nonadherence to radiation therapy schedules is a documented problem among head and neck cancer patients. This retrospective dissertation study examined whether demographics, clinical characteristics, or physical and psychological symptoms were related to nonadherence in head and neck cancer patients. The electronic medical records of 262 head and neck cancer patients at a southeastern U.S. cancer center were reviewed to determine whether nonadherence was related to symptom scores and other patient and clinical-related factors. Nonadherent patients were more likely to be female, be admitted to the cancer center as inpatients during treatment and receive outpatient IV fluids during treatment. Nonadherent patients …

Anorexia Nervosa And Bulimia Nervosa As Expressions Of Shame In A Post-Feminist, Emily Kearns

USF Tampa Graduate Theses and Dissertations

Anorexia nervosa and Bulimia nervosa are heavily gendered conditions affecting women to a far greater degree than men in a post-millennium Western setting. The psychologistic and medicalized approaches to studying and treating these disorders do not account for socio-cultural and epistemic preferences. This paper draws a connection between shame (as emotion and affect) and these gendered disorders. Further, this work analyzes neo-liberalism, post-feminism, and consumerism as predatory elements of Western culture especially affecting women.

Social Exclusion Of Older Mossi Women Accused Of Witchcraft In Burkina Faso, West Africa, Clarisse Barbier

USF Tampa Graduate Theses and Dissertations

Among the most marginalized populations in the world, one group of women has been persistently ignored, silenced, and forgotten. In Burkina Faso, West Africa, older women in rural villages are often the target of witchcraft accusations; the consequences of these accusations are alarming because these women undergo violent attacks, face exclusion from their villages, and become the most vulnerable and marginalized segment of the Burkinabe population. Between August 2017 and November 2018, I conducted an ethnographic study of Burkinabe women accused of witchcraft living in two shelters in the capital city of Ouagadougou and examined women’s experiences of accusation, trauma, …

Exploring The Relationship Between Pre-Medical Student Motivation And Academic Performance: Strategies To Improve Student Success, Lauren Albaum

USF Tampa Graduate Theses and Dissertations

Colleges and universities are under increasing pressure to meet post-baccalaureate graduate and professional school outcomes, with the majority of students indicating their central reason for attending college is to “landing a better job” (Eagan et al., 2016). Thus, even as interest in medical school and veterinary medical school continues to rise (Eagan et al., 2016), thereby increasing the competition to obtain an acceptance, students and parents continue to hold an expectation that their undergraduate institution will adequately prepare them to successfully matriculate into medical school or veterinary medical school. Although a variety of factors influence acceptance to professional school, earning …

Breakthroughs In Obtaining Qm/Mm Free Energies, Phillip S. Hudson

USF Tampa Graduate Theses and Dissertations

The computation of free energy is pivotal to understanding the fundamental nature of chemical phenomena. That is, whether a specific molecular outcome occurs spontaneously or is inherently unfavorable. The need to do this with consistent accuracy begs for the use of quantum mechanical (QM) methods. However, techniques for directly computing free energy differences with QM or mixed QM/MM methods are untenable, as the computational expense is quite exorbitant. At present, the most feasible approach for obtaining QM/MM free energies is employing the so-called indirect cycle, which relies on accurately computing free energy differences between low (e.g., molecular mechanical, MM) and …

Simulation And Verification Of Phase Change Materials For Thermal Energy Storage, Marwan Mosubah Belaed

USF Tampa Graduate Theses and Dissertations

Greenhouse gas (GHG) emissions have a global impact on world economies and the ecosystem. Scientific assessment of GHG emissions is an issue that requires immediate action from key decision-makers and influencers. A comprehensive review of the impact of climate change and its dire consequences on daily life is illustrated in this dissertation. The most common technique to lower GHG emissions in the atmosphere is by switching GHG emission sources to zero-emission sources such as solar energy, wind energy, bioenergy, and geothermal power. Recently, all these technologies have made significant progress in lowering capital operation costs and their cycle efficiencies. This …

Laser Micropatterning Effects On Corrosion Resistance Of Pure Magnesium Surfaces, Yahya Efe Yayoglu

USF Tampa Graduate Theses and Dissertations

Magnesium and its alloys are good candidates to manufacture medical implants. They have excellent biocompatibility and because they biodegrade secondary surgical operation to remove the implant could be eliminated. However, in aqueous environments, magnesium alloys rapidly corrode, resulting in premature degradation of the implant along with biologically intolerable hydrogen gas generation. In literature, there are multiple studies focused on creating water repelling hydrophobic magnesium surfaces in order to decrease corrosion rates. Hydrophobic properties can be achieved by creation of a roughness profile on an initially smooth surface combined with a treatment that reduces the free surface energy. In theory, hydrophobic …

Manipulation And Patterning Of Mammalian Cells Using Vibrations And Acoustic Forces, Joel Cooper

USF Tampa Graduate Theses and Dissertations

Recently, there has been a surge in researchers and scientists investigating different methods which move, manipulate, and pattern biological cells. Multiple different mechanisms can be used for cellular manipulation, microfluidics, biochemical queues, and even optics, just to name a few. However, all techniques have their downsides. A majority of these methods require expensive equipment or reagents and can only manipulate a small number of cells at a time.

Some of the most common cell manipulation devices utilize acoustic pressure waves to move the cells to desired locations. Currently, it is unknown what level of force from these types of devices …

New Synthetic Methodology Using Base-Assisted Diazonium Salts Activation And Gold Redox Catalysis, Abiola Azeez Jimoh

USF Tampa Graduate Theses and Dissertations

Over the past two decades, homogeneous Au(I) catalysis has been a valuable synthetic tool for activating a series of unsaturated carbon-carbon bonds towards nucleophilic attack. Despite this progress, Au(I/III) redox chemistry has not been well explored. This is largely due to the challenges associated with the high oxidation potential of gold. Hence, the need for an efficient and safe method for Au oxidation.

Herein, a base-assisted diazonium salt activation has been used to facilitate Au redox reactions under mild reaction conditions. This oxidative coupling method afforded functional isoxazolines with good to excellent yields, while the alternative photoactivation method provided trace …

“They Will Think We Are The Cancer Family”: Studying Patterns Of Cancer Disclosure And Communication Among Indian Immigrants In The United States, Kanan Mehta

USF Tampa Graduate Theses and Dissertations

Studies of Indian immigrants in Western countries show that the rates of cancer increase significantly within a generation in the host country. The negative social perceptions associated with health outcomes of cancer often perpetuate limited disclosure regarding the diagnosis of cancer among patients and families. This can result in disrupted communication in clinical settings, while causing increased stress among patients and caregivers. These findings demonstrate the need for studying lived experiences of cancer-related illness and its impacts on social relationships in the domestic and public sphere.

This study explored cancer disclosure and communication among Indian immigrants in the United States …

Study Of The Therapeutic Effects Of Synchronization-Modulation Of The Na/K Pump On Muscle Fatigue, Jason E. Mast

USF Tampa Graduate Theses and Dissertations

It has been shown that by applying a specially designed oscillating external electric field to a cell membrane that the membrane's sodium/potassium pumps can be synchronized to all work at the same rate. Then by slowly increasing the electric field's frequency the pumps' turnover rate can also be increased. By increasing the pumps' turnover rate, the sodium and potassium concentration gradients can be increased, this type of stimulation is called synchronization-modulation. There are three generations of the synchronization-modulation waveform each with different utilities. In particular, the third generation of synchronization-modulation has the ability to use the energy of the external …

Hybrid Electro-Plasmonic Stimulation Of Primary Neurons, Ratka Damnjanovic

USF Tampa Graduate Theses and Dissertations

Biomedical prosthetics utilizing electrical stimulation have limited, effective spatial resolution due to spread of electrical currents to surrounding tissue, causing nonselective stimulation. So, precise spatial resolution is not possible for traditional neural prosthetic devices, such as cochlear implants. More recently, alternative methods utilize optical stimulation, mainly infrared, sometimes paired with nanotechnology for stimulating action potentials, which has its own drawbacks, as it may heat surrounding tissue. Recently, we employed plasmonic stimulation methods utilizing gold nanoparticle-coated nanoelectrodes to convert visible light pulses into localized surface plasmon resonance transduction for stimulation of electrically excitable cells, which had limited success. Here, we report …

Parent Perceptions Of The Acceptability, Effectiveness, And Experience Of Engaging In The Group Stepping Stones Triple P Intervention For Parents Of Children With Disabilities, Tara B. Delach

USF Tampa Graduate Theses and Dissertations

The challenges associated with parenting are often compounded for parents of children with developmental disabilities. Children with developmental disabilities are at increased risk for exhibiting mental health concerns and challenging behavior compared to their typically developing peers. Parents who are raising a child with a disability tend to experience increased demands, higher levels of stress, and greater challenges associated with the physical, emotional, and behavioral needs of their children than do parents of typically developing children. Parent training interventions grounded in social learning theory and behavioral principles have proven to be effective in improving both child and parent outcomes in …

Allosteric Control Of Proteins: New Methods And Mechanisms, Nalvi Duro

USF Tampa Graduate Theses and Dissertations

Allostery describes the phenomenon where perturbations in one region of a protein affect protein behavior in another non-overlapping region. Considerable efforts made over decades to understand the molecular basis of allostery, yet an overarching theory that can predict signaling pathways and contributions from chemical components is still lacking. In fact, molecular details in even the most well-studied of model systems, PDZ domains and GPCRs, remain unclear. In this dissertation I use molecular simulation methods to understand the role of allostery in the regulated entry of paramyxovirus into host cells, and also develop a new method to determine time-dependent signaling pathways …

Developing Agent-Based Models To Study Financial Markets, Saurav Chakraborty

USF Tampa Graduate Theses and Dissertations

This dissertation presents research that employs agent-based modelling to provide a framework to support simulation as a complement to traditional economic models for policy evaluation. It consists of three studies. The first study employs cluster analysis to capture the different types of banks and the associated business models that define their decision-making. The results from study one will help us get an understanding of how different banks behave and provide an insight into their lending practices. Hence, it would be very helpful in evaluating and analyzing the impact of future policies. Study two develops a fine-grained interbank lending model based …

The Role Professional Development Schools Play In Building Personal And Collective Teacher Efficacy, Janine L. Hall

USF Tampa Graduate Theses and Dissertations

The purpose of this case study was to explore the perceptions of members of three triads currently working in a professional development school (PDS). to determine in what ways the context of the PDS influenced personal or collective efficacy. The triads consisted of a collaborating teacher, pre-service teacher, and university supervisor and were all located in one professional development school. Data were collected from two face-to-face individual interviews with each triad member and one focus group meeting with all triad members. Findings revealed that the growth of efficacy was influenced by the focus the PDS placed on student learning and …

Making Meaning Of A Whole School Transformation Model: A Policy Ethnography Of A Schools Of Hope Legislation Implementation, Adam Charles Rea

USF Tampa Graduate Theses and Dissertations

Cheryl High School (CHS) is a geographically large county in Florida that received funding through the state of Florida's "Schools of Hope” grant. The grant’s parameters called on Carter County Schools (CCS) to partner with an external consultant and a research university to help improve teacher and school administrative capacity. The district chose Scientific Pedagogy Services (SPS) and to continue their existing relationship with Tampa Bay State University (TBS). The consultant’s mission was to improve school performance through a unique form of professional development. TBS was charged with developing an on-site program that funded staff efforts to obtain advanced degrees …

Understanding The Role Of Cereblon In Hematopoiesis Through Structural And Functional Analyses, Afua Adutwumwa Akuffo

USF Tampa Graduate Theses and Dissertations

The discovery and implementation of immunomodulatory drugs (IMiD®s) has revolutionized the treatment of many hematological malignancies due to the plethora of IMiD®-induced clinical responses that include anti-angiogenesis, anti-inflammation, and anti-tumor effects, as well as enhanced erythropoiesis, immune modulation and improved metabolism. More ground-breaking was the identification of cereblon as the target of IMiD®s. Upon binding to thalidomide and other immunomodulatory drugs, the E3 ligase substrate receptor cereblon (CRBN) promotes proteosomal destruction of neo-substrates by engaging the DDB1-CUL4A-Roc1-RBX1 E3-ubiquitin ligase in human cells but not in mouse cells suggesting that sequence variations in CRBN may cause its inactivation. Therapeutically, CRBN engagers …

Game Theory Approaches For Transportation Problems, Mahdi Takalloo

USF Tampa Graduate Theses and Dissertations

This dissertation considers three separate game theory problems in transportation. In the first problem, a combinatorial auction market has been proposed for fractional ownership of autonomous vehicles. The proposed combinatorial auction has two unique features. First, the items are continuous time slots defined by bidders and second, the spatial information of bidders has been incorporated so that sharing becomes a viable plan. A conflict-based formulation of the winner determination problem has been proposed, for which an effective solution approach based on a heuristic and a maximal-clique based relaxation has been presented. The second part of the dissertation examines a pessimistic …

Polycyclic Aromatic Hydrocarbon Exposure, Hepatic Accumulation, And Associated Health Impacts In Gulf Of Mexico Tilefish (Lopholatilus Chamaeleonticeps), Susan M. Snyder

USF Tampa Graduate Theses and Dissertations

Following the 2010 Deepwater Horizon oil spill, systematic demersal longline surveys were conducted throughout the Gulf of Mexico (GoM) continental shelf to evaluate polycyclic aromatic hydrocarbon (PAH) exposure, hepatic accumulation, and health indices in demersal fishes. Tilefish (Lopholatilus chamaeleonticeps) were chosen as a target species due to high vulnerability to environmental disturbance, commercial importance, Gulf-wide distribution, and documented high exposure to PAHs post-Deepwater Horizon. Over 200 Tilefish were sampled in the north central GoM at repeat stations from 2012 to 2017, and from the northwest GoM, southwest GoM, Bay of Campeche, and Yucatán Shelf over years 2015 and 2016. Tilefish …

Governmentality, Biopower, And Sexual Citizenship: A Feminist Examination Of Sexual And Reproductive Healthcare Experiences Of 18-24 Year-Olds In The U.S. Southeast, Melina K. Taylor

USF Tampa Graduate Theses and Dissertations

Sexual and reproductive healthcare in the U.S. is a contentious and often stigmatized topic. Conservative politics and Christian religious ideology guide laws and policies that inform narratives of sexual citizenship that promote white, heterosexual, procreative, cis-gendered relationships as the ideal. For young people, exposure to sexuality education greatly influences their self-identity as sexual citizens and guides how they form intimate relationships. While sexual and reproductive healthcare has been included marginally in the discipline of anthropology, almost no research has focused on young people’s sexual and reproductive healthcare within the U.S.

This dissertation examines the viewpoints and experiences of 18-24 year-old …

Nostalgia And (In)Authentic Community: A Bataillean Answer To The Heidegger Controversy, Patrick Miller

USF Tampa Graduate Theses and Dissertations

Heidegger’s relationship with Nazism has been debated since the 1930s. In the late 1930s, Georges Bataille wrote an incomplete text that would have added to these debates, “Critique of Heidegger: Critique of a philosophy of fascism.” I draw on this fragment and Bataille’s writings from this era in order to develop a fuller critique of Heidegger and his relationship to fascism. This expanded critique completes the promise of Bataille’s original fragment, offering a full Bataillean criticism of Heidegger and displaying the connections between his philosophy and Bataille’s understanding of fascism. This critique hinges on Heidegger’s concept of authenticity and community, …

Investigating Mechanisms Of Immune Suppression Secondary To An Inflammatory Microenvironment, Wendy Michelle Kandell

USF Tampa Graduate Theses and Dissertations

The immune system plays a dynamic role in cancer progression. The theory of immunoediting suggests that the relationship between the tumor cell and the immune cell is one that is in flux: initially highly active and responsive to tumor antigen to one that has escaped immune responsiveness. Once the tumor has formed and effectively “escaped”, there are multiple mechanisms that work against a conventional immune response. The tumor cell clones that have escaped the elimination phase are those that are less immunogenic. These clones downregulate MHC, have increased apoptosis through DAMP-driven mechanisms, and suppressive cell phenotypes are driven through cytokine-driven …

A Novel Intramolecular Interaction In P53, Fan He

USF Tampa Graduate Theses and Dissertations

The p53 tumor suppressor is a sequence-specific DNA binding protein that activates gene transcription to regulate cell survival and proliferation. The activation process involves post-translational modifications that suppress p53 degradation by MDM2 and increase p53 DNA binding affinity. p53 is mutated in ~50% of human tumors, with higher frequency in specific tumor types and after relapse. Mutated p53 loses transcriptional activity and gains new functions that drive tumor progression. Both N-terminus (NT) and C-terminus (CT) of p53 contain intrinsically disordered regions. The p53 CT has well-documented effects in regulating DNA binding. CT truncated p53 mutants showed defective DNA binding and …