Digital Commons Network^™

Health Effects Of Oil Spills And Dispersal Of Oil Droplets And Zooplankton By Langmuir Cells, Sanjib Gurung

USF Tampa Graduate Theses and Dissertations

This Ph.D. dissertation deals with the study of the health effects of crude oil vapors from oil spills, the effect of Langmuir circulation on the dispersion of oil droplets during oil spill events, and the biological significance of Langmuir circulation. The introduction to areas of three major research projects undertaken during my Ph.D. is included in chapter 1 and the relevant literature review is included in chapter 2. The first research project described in chapter 3 involves the quantification of health effects on embryonic Gulf killifish due to exposure to crude oil vapors. The second research project described in chapter …

Community Scholars: A Diversity Pipeline Program To Promote Equity, Diversity, And Inclusion For People With Disabilities, Laura M. Rodríguez López

USF Tampa Graduate Theses and Dissertations

This dissertation in practice documents the program evaluation of the Community Scholars (CS) program at the Florida Center for Inclusive Communities (FCIC), a University Center for Excellence in Developmental Disabilities (UCEDD) at the University of South Florida (USF). CS is part of a diversity pipeline program that recruits youth with disabilities to engage in paid on-the-job training (OJT) with FCIC. Scholars are community members (youth with disabilities) that are enrolled as FCIC trainees and receive mentoring, development of basic administrative competencies, individualized career coaching, college counseling, and disability policy and self-advocacy training. Through the OJT experience, scholars gain skills that …

Coarse-Grained Modeling Studies Of Entangled Semiflexible Polymers: Melts, Glasses, And Granular Media, Joseph Fox D. Dietz

USF Tampa Graduate Theses and Dissertations

This dissertation is devoted to the computational study of model semiflexible polymers using a coarse-grained bead-spring approach and molecular dynamics (MD) simulations. Much of the work presented here is motivated by the need to derive predictive models for describing polymeric materials’ macroscopic mechanical response from the underlying local structure or local chain parameters. This dissertation describes polymeric materials in the context of granular media, melts, and glasses with a focus on relating characteristics such as chain stiffness and local structure to systems’ solidification, rheology, and fracture mechanisms.

Chain stiffness κ and structural properties like chains’ characteristic bond angle θ0 are …

Do Sociability Expectancies Moderate Social Anxiety Predicting Alcohol Consumption Following A Social Stressor Speech Task, Jacob A. Levine

USF Tampa Graduate Theses and Dissertations

Alcohol use disorder (AUD) and social anxiety disorder (SAD) are two of the most common and chronic psychiatric conditions in the United States. Research indicates individuals with SAD are more likely to meet lifetime criteria for AUD and experience greater AUD symptomatology and severity. Theories explaining this connection are limited, with most built upon tension reduction principles not specific to SAD; however, a recent biopsychosocial model focuses on factors specific to SAD, such as drinking to cope with states of high negative affectivity and arousal. Despite high rates of comorbidity, and more severe AUD presentation, the literature is mixed regarding …

African American Males' Perception Of Factors That Contribute To Success In Higher Education, Gary D. Oliver

USF Tampa Graduate Theses and Dissertations

Over the past decades, many studies have concluded that African American students' college completion rate and success lag far behind other students attending college in the United States (The JBHE Foundation, Inc., 2006). More specifically, these studies have confirmed that African American male students' success rates remain disproportionally low compared to other ethnic male groups. Unfortunately, few notable studies identifying African American males achieving higher education or completing their academic pursuits have been presented as part of the Black male student narrative.

This study aimed to understand better the resources and experiences that positively affect African American males who completed …

Utilizing Maternal Investment Strategies Of Sharks To Develop Management Tools To Aid In Shark Conservation, Dennis Deeken

USF Tampa Graduate Theses and Dissertations

Every generation, breeding females and males repopulate ecosystems with the next generation of offspring. Two models link maternal investments in offspring size and number to natural selection processes. In unstable resource environments, bet-hedging models predict a conservative tradeoff in maternal investments resulting in moderate egg size and clutch size within a given range of sizes for a given species. r/K selection models make predictions on maternal investments across species. In unstable resource environments, r-selected species invest in large broods of small offspring. In stable environments, K-selected species invest in small broods of large offspring. The maternal risk management model predicts …

To Be Or Not To Be: A Tale Of Staphylococcal Gpsb, Lauren R. Hammond

USF Tampa Graduate Theses and Dissertations

There are two big drivers motivating studies into bacterial cell division. The first is a desire to understand life around us. One of the defining characteristics of life is the ability for a cell to grow and divide, and without in-depth knowledge of this process, we cannot truly understand the complexities that allow for life on this planet. The second motivator is to identify new drug targets in the ongoing fight against antimicrobial resistance. Many infectious organisms have become resistant to commonly used antibiotics, and the bacterial cell division machinery is a largely untapped essential process with many potential therapeutic …

Lord Of The Z-Rings: Uncovering The Role Of Mraz And Ftsl In Bacillus Subtilis Cell Division, Maria Louise White

USF Tampa Graduate Theses and Dissertations

Cell division is a complex and highly orchestrated process that requires careful coordination. The process of cell division in prokaryotes primarily occurs via binary fission, where one mother cell becomes two genetically identically and equally sized daughter cells. In most bacteria a key protein, FtsZ, forms a FtsZ ring (Z-ring) to mark the division site and recruit proteins involved in cytokinesis. Whilst much has been learned about the contents and assembly of the divisome in model organisms such as Escherichia coli and Bacillus subtilis factors governing the regulation of divisome are much more poorly understood. …

Design And Assessment Of A Novel Edna Survey Method For The Eastern Indigo Snake (Drymarchon Couperi), Emily K. Galbraith

USF Tampa Graduate Theses and Dissertations

1. Accurate information about species range declines is the cornerstone of effective conservation when habitat loss drives biodiversity loss. The Eastern Indigo snake (Drymarchon couperi) is an apex predator in Florida and, like many other native Florida species, is threatened by widespread habitat destruction. Environmental DNA (eDNA) monitoring of this elusive snake would provide a low-risk and low-cost approach to expand our knowledge of the species’ range and distribution.

2. We designed and tested an eDNA assay that can detect the presence of Eastern Indigo snake from soil samples of their natural habitat. This assay was …

Effect Of Hyperoxia On Cardiac Pathophysiology In Female Guinea Pig Hearts, Chayapatou Chayawatto

USF Tampa Graduate Theses and Dissertations

Hyperoxia is widely implemented in critical care and ICU patients. The administration of a high concentration of inspired oxygen to the lung can unknowingly cause hyperoxia and thereby damaging the lungs and heart due to oxidative stress. Technically, hyperoxia occurs when the patient receives PaO2 > 200 mmHg. Major research focused on hyperoxia-induced lung injury, but nothing is known on its effect on heart. Our lab is the pioneer in understanding the effect of hyperoxia on cardiovascular remodeling using mice model. Previous results show that mice under 72 hours of hyperoxia present severe cardiac pathophysiology. This research uses Guinea pigs, which …

Interactions Between Juvenile Estuary-Dependent Fishes And Microalgal Dynamics, Ian C. Williams

USF Tampa Graduate Theses and Dissertations

The ontogenetic movement of juvenile estuary-dependent fishes upstream in estuarine settings is a phenomenon observed around the world. These movements usually coincide with a shift in diet from smaller prey, such as calanoid copepods, to larger prey, such as mysids. However, the mechanism that drives this pattern has yet to be conclusively described. Prior to the current study, zooplankton/hyperbenthos (“zooplankton”), primary production, and water quality data were collected from the Caloosahatchee River estuary in two concurrent and coordinated studies over a two-year period. One of the products of these sampling efforts was a classification of primary-producer types at the sediment-water …

Ec Expression Of Activated K-Ras Leads To Capillary Deficiency Due To Excessive Lumen Formation, Reduced Pericyte Recruitment, And An Increased Susceptibility To Mmp-1-Dependent Vascular Regression, Zheying Sun

USF Tampa Graduate Theses and Dissertations

Activated k-Ras mutations play a role in a variety of pathological conditions, such as arteriovenous malformations (AVMs). To study the effect of activated k-Ras mutation on lumen formation, we engineered human ECs to simultaneously express the activated k-Ras (G12V) mutation and compared their ability to form lumens with control ECs. We demonstrated that ECs with k-RasV12 lead to marked stimulation of EC lumen formation due to dramatic increases in intracellular vacuole formation and fusion, signaling changes including accelerated Src kinase activation, and MT1-MMP-dependent lumen expansion, all of which drive the process of lumen formation. The markedly accentuated lumen formation is …

Exploring Parenthood And The Influence Of Lived Experiences On Parenting Behaviors Among Child Welfare-Involved Adults, Veronica Howell

USF Tampa Graduate Theses and Dissertations

The World Health Organization asserts the healthy development of children is essential. Cases of violence and maltreatment impede the development of children worldwide. Six in 10 adults in the general US population have reported at least one adverse childhood experience (ACE), while vulnerable populations, including child welfare-involved adults (i.e., parents and caregivers), often report more. This history is associated with higher morbidity and mortality rates alongside higher economic costs; therefore, preventing ACEs and promoting safe, stable, and nurturing relationships between parents and children is critical. The purpose of this exploratory qualitative study was to understand the meanings and responsibilities associated …

Measurement In Chemistry, Mathematics, And Physics Education: Student Explanations Of Organic Chemistry Reaction Mechanisms And Instructional Practices In Introductory Courses, Brandon J. Yik

USF Tampa Graduate Theses and Dissertations

The work in this dissertation is presented in two parts. The first part (Chapters 3 and 4) details work relating to students’ explanations of reaction mechanisms in organic chemistry. The second part (Chapters 5 and 6) details work relating to the evaluating the uptake of research-based instructional practices in introductory chemistry, mathematics, and physics courses.

To evaluate learning of organic chemistry reactions, instructors must ask students to construct written explanations of reaction mechanisms. Written assessments should focus on what is happening and why it is happening to promote deeper student understanding. However, for instructors to gain insight into students’ understanding, …

Accelerating Multiparametric Mri For Adaptive Radiotherapy, Shraddha Pandey

USF Tampa Graduate Theses and Dissertations

MR guided Radiotherapy (MRgRT) marks an important paradigm shift in the field of radiotherapy. Superior tissue contrast of MRI offers better visualization of the abnormal lesions, as a result precise radiation dose delivery is possible. In case of online treatment planning, MRgRT offers better control of intratumoral motion and quick adaptation to changes in the gross tumor volume. Nonetheless, the MRgRT process flow does suffer from some challenges that limit its clinical usability. The primary aspects of MRgRT workflow are MRI acquisition, tumor delineation, dose map prediction and administering treatment. It is estimated that the acquisition of MRI takes around …

Organizing For Here And There: Exploring The Grassroots Organizing Of The Puerto Rican Diaspora In The Tampa Bay Area, Dominique Rivera

USF Tampa Graduate Theses and Dissertations

Drawing upon participant observations and semi-structure interviews with 10 Puerto Rican grassroots organizers from the Tampa Bay area of Florida, this project examines the processes by which Puerto Rican diaspora members build, maintain, and utilize social and symbolic ties as resources for organizing and executing grassroot projects and campaigns with a dual focus on the Puerto Rican community in the Tampa Bay area and in Puerto Rico. Complex webs of interlocking social and symbolic ties that transcend region of origin and regions of destination constitute a transnational social field, within which exchanges of ideas, practices, and resources are organized among …

Social Media Time Series Forecasting And User-Level Activity Prediction With Gradient Boosting, Deep Learning, And Data Augmentation, Fred Mubang

USF Tampa Graduate Theses and Dissertations

In the overall history of technological innovations, social media has only existed for a brief time, however its influence is undeniable. Researchers have found that it can be used to influence elections, spread health misinformation, and aid with financial pump-and-dump schemes. Keeping all this in mind, it is clear that more research is needed to predict the spread of information on social media in order to combat its malicious use.

To that end, in this dissertation, we explore the use of Machine Learning algorithms to perform time series forecasting and user-level activity prediction in social media. We address the different …

Association Between Use Of Remdesivir And Bradycardia, Gibret Umeukeje

USF Tampa Graduate Theses and Dissertations

Remdesivir received the first emergency use authorization from the FDA for the treatment of COVID-19. Multiple adverse drug reactions (ADR) have been reported since its approval in October 2020. Bradycardia, defined by a decrease in heart rate has been reported as an adverse event for patients receiving remdesivir for COVID-19 treatment. The purpose of the research is to systematically investigate the frequency of occurrence of bradycardia in adults receiving remdesivir using clinical data derived from the FDA Adverse Event Reporting System (FAERS) database. Patients receiving remdesivir were compared to those receiving Paxlovid, Regen-Cov, and Dexamethasone for COVID-19 treatment to see …

Going Flat: Challenging Gender, Stigma, And Cure Through Lesbian Breast Cancer Experience, Beth Gaines

USF Tampa Graduate Theses and Dissertations

This paper explores the decision-making process of reconstruction surgery among lesbian breast cancer patients to better understand how identity impacts healthcare decisions. Breast cancer patients experience the disease in unique ways due to gender, sexuality, race, and class, impacting their individual decisions regarding treatment plans. Many breast cancer patients face mastectomy surgery as the first plan of treatment after diagnosis. By exploring the impact of gender, sexuality, stigma, and ideas of cure, this research aims to advance research about breast cancer by recognizing why some lesbian breast cancer patients forego reconstruction surgery and instead choose to “go flat.

Adaptive Multi-Scale Place Cell Representations And Replay For Spatial Navigation And Learning In Autonomous Robots, Pablo Scleidorovich

USF Tampa Graduate Theses and Dissertations

Place cells are one of the most widely studied neurons thought to play a vital role in spatial cognition. Extensive studies show that their activity in the rodent hippocampus is highly correlated with the animal’s spatial location, forming “place fields” of smaller sizes near the dorsal pole and larger sizes near the ventral pole. Despite advances, it is yet unclear how this multi-scale representation enables navigation in complex environments.

In this dissertation, we analyze the place cell representation from a computational point of view, evaluating how multi-scale place fields impact navigation in large and cluttered environments. The objectives are to …

Screening Pathological Protein Specific Antibodies As Biomarker Discovery Tools For Neurodegenerative Disease, Yitong Li

USF Tampa Graduate Theses and Dissertations

With the increase in the average life span, the incidence of neurodegenerative diseases also increases, among which Alzheimer's disease (AD) and Parkinson's disease (PD) are the two most common types of neurological disorders. AD and PD share the same pathogenic mechanism that involves a class of proteins called beta-amyloid (Aβ), which, under the induction of some factors, changes from its natural monomeric state to the oligomers containing β-sheet, which is neurotoxic and thus leads to cell damage or apoptosis. This study focused on the selection of antibodies used as biomarkers for AD and PD. The selection of antibodies was based …

A Holistic Investigation Of Acidosis In Breast Cancer, Bryce Ordway

USF Tampa Graduate Theses and Dissertations

In the early 20th century, Nobel laureate Otto Warburg made the observation that cells of a carcinoma had considerably higher glycolytic metabolism and considerably lower oxidative metabolism compared to cells of a normal tissue. He postulated that within this observation was the key to deciphering the differences between malignant and normal tissue. It is now well established that tumors of the breast are unequivocally acidic, caused by an abnormal amount of aerobic glycolysis, colloquially known as the Warburg effect. Over the last decades, our group, led by Dr. Robert J. Gillies, has set out to characterize the causes and consequences …

Towards High Performing And Reliable Deep Convolutional Neural Network Models For Typically Limited Medical Imaging Datasets, Kaoutar Ben Ahmed

USF Tampa Graduate Theses and Dissertations

Artificial Intelligence (AI) is “The science and engineering of making intelligent machines, especially intelligent computer programs”. Artificial Intelligence has been applied in a wide range of fields including automobiles, space, robotics, and healthcare.

According to recent reports, AI will have a huge impact on increasing the world economy by 2030 and it's expected that the greatest impact will be in the field of healthcare. The global market size of AI in healthcare was estimated at USD 10.4 billion in 2021 and is expected to grow at a high rate from 2022 to 2030 (CAGR of 38.4%). Applications of AI in …

Beliefs, Identity, And An African American Cemetery: An Exploratory Study Of Difficult History Curricular Decision- Making, Shannon Peck-Bartle

USF Tampa Graduate Theses and Dissertations

In this qualitative exploratory study, I examine the influence of administrative curricular decision-makers’ beliefs and values towards race and ethnicity, heritage, and place on curricular aims for the inclusion of local difficult history associated with the erasure of a racialized cultural landscape, The Ridgewood Cemetery. I additionally examine the influence of contemporary issues on beliefs and values as administrative curricular decision-makers navigate ways to incorporate local cemetery history into secondary social studies curriculum. Through semi-structured interviews, document analysis, and research’s reflective journaling I shed light on ways beliefs, values, and contemporary issues influenced administrative curricular decision-making for local difficult history. …

Gender Differences In College Drinkers: The Role Of Masculine Norms, Jared A. Davis

USF Tampa Graduate Theses and Dissertations

Drinking among college students has remained a prominent problem within the United States, with more than 50% of college students drinking alcohol, 30% considered binge drinkers, and 9% considered heavy drinkers (SAMHSA, 2018). Evidence also shows that males are more likely to partake in risky drinking behaviors (e.g., binge drinking or drinking to intoxication) and are at higher risk to be diagnosed with a alcohol use disorder when compared to women (Iwamoto et al. 2014; Grant et al., 2004). Recent findings suggest that adherence to particular masculine norms as a risk factor for problematic alcohol use among men (Mahalik, 2000; …

X-Linked Ubiquitin-Specific Peptidase 11 Increases Tauopathy Vulnerability In Women, Yan Yan

USF Tampa Graduate Theses and Dissertations

Women experience significantly higher tau burden and increased risk for Alzheimer’s disease (AD) than men, yet the underlying mechanism for this vulnerability has not been explained. Here, we demonstrate through in vitro and in vivo models, as well as human AD brain tissue, that X-linked ubiquitin specific peptidase 11 (USP11) augments pathological tau aggregation via tau deubiquitination initiated at lysine-281. Removal of ubiquitin provides access for enzymatic tau acetylation at lysines 281 and 274. USP11 escapes complete X-inactivation, and female mice and people both exhibit higher USP11 levels than males. Genetic elimination of usp11 in a tauopathy mouse model preferentially …

Hidden Struggles Within The Mental Healthcare Workplace, Tammy V. Smith

USF Tampa Graduate Theses and Dissertations

For decades, the Centers for Disease Control and Prevention (CDC) and World Health Organization (WHO) have reported on the worldwide pervasiveness and increasing societal costs associated with Adverse Childhood Experiences (ACEs). A recent post on the WHO website cited recent scientific research (2022) indicating the magnitude of harmful effects attributed to ACEs, even classifying them as “violence against children.” Esaki and Larkin (2013) indicate that ACEs are prevalent among many social service professionals, including mental health clinicians.

This study uses mental health clinicians as the sample population to identify the impact of ACEs on job performance. Findings from a volunteer …

Neural And Cardiac Mechanisms In Friedreich's Ataxia With Patient-Derived Ipscs, Mariana Burgos Angulo

USF Tampa Graduate Theses and Dissertations

Friedreich's ataxia (FA) is an autosomal recessive disease caused, in most cases, by a GAA trinucleotide repeat expansion in the first intron of the frataxin (FXN) gene, which results in transcriptional repression of the encoded protein frataxin. FA is a progressive neurodegenerative disorder, but the primary cause of death is hypertrophic cardiomyopathy, which occurs in 60% of the patients. Several functions of frataxin have been proposed, but none of them can fully explain why its deficiency causes the FA phenotypes nor why the most affected cell types are neurons and cardiomyocytes. It is possible that frataxin affects neural and cardiac …

Chemical Investigation And Drug Discovery Potential Of Terpenoid Secondary Metabolites From Three Deep-Sea Irish Soft Corals, Joshua Thomas Welsch

USF Tampa Graduate Theses and Dissertations

Secondary metabolites produced primarily by terrestrial plants and animals have provided the basis for treatments and cures of human diseases as far back as historians have been able to probe. However, recent technological advances including the self-contained underwater breathing apparatus, and even further remotely operated vehicles, have allowed researchers a unique opportunity for targeted collection of marine organisms at depths previously thought to possess conditions unsustainable for life as was understood. This has afforded a glimpse into the potential for not only life, but the potential for new biochemical adaptations developed over billions of years that have been evolutionarily developed …

Remnants Of Educational Leadership And Desegregation Etched In The Memories Of Black Educational Leaders: An Oral History, Janice Barge Clarke

USF Tampa Graduate Theses and Dissertations

In this study the experiences of Black (a.k.a. African Americans/ Negroes) educationalleaders were explored focusing on the period during the transition to a more desegregated public- school setting in the state of Florida. Using retrospective storytelling and reflections of ‘leading’ during desegregation, the lived experiences of those in educational leadership roles were captured in the form of oral histories and analyzed using critical race theory. The effects of desegregation is recounted from their vantage point, from the dissolution of the ‘all Black’ schools to the impact it had on the communities. The research question was: What are the stories told …