Digital Commons Network^™

Systematic Study Of Amyloid Beta Peptide Conformations: Implications For Alzheimer’S Disease, Jeffy Pilar Jimenez

USF Tampa Graduate Theses and Dissertations

The amyloid beta peptide particularly the 40 and 42 amino acid residues are the responsible for plaque formation in Alzheimer's disease (AD) patients. Extra cellular plaque formation has been recognized after incessant investigations along with the formation of intracellular tau protein tangles as the hallmarks of AD. Furthermore, the plaque formation has been linked mostly as a cause of the disease and the tangles mostly as a consequence. Our investigation is focused on studying the formation of AD plaques. The amyloid beta (Aß) is a physiological peptide secreted from neurons under normal conditions, along with other soluble forms cleaved from …

Performativity And The Latina/O-White Hybrid Identity: Performing The Textual Self, Shane T. Moreman

USF Tampa Graduate Theses and Dissertations

This study is an exploration of Latina/o-White hybrid identity for constructions and negotiations of hybridity as performed in the lives of individuals and as rearticulated in discourse. These discourses are drawn from interviews with nine individuals, stories of my own life, and three published memoirs. Despite these different forms, all the selfidentified Latina/o-White hybrid individuals speak to the difficulty of imagining and enacting a hybrid identity within today’s discourse on race and ethnicity. This study articulates these difficulties as lived experience, theory, and performance come together to argue for and against hybridity as a model for contemporary identity. The project …

Polytetrahydrofuran-And Dendrimer-Based Novel Sol-Gel Coatings For Capillary Microextraction (Cme) Providing Parts Per Trillion (Ppt) And Parts Per Quadrillion (Ppq) Level Detection Limits In Conjunction With Gas Chromatography And Flame Ionization Detection (Fid), Abuzar Kabir

USF Tampa Graduate Theses and Dissertations

Sol-gel capillary microextraction (CME) is a new direction in solvent-free extraction and preconcentration of trace analytes. CME presents significant interest in environmental, pharmaceutical, petrochemical, biomedical, agricultural, food, flavor, and a host of other important areas. Sol-gel CME utilizes advanced material properties of organic-inorganic hybrid sol-gel polymers to perform efficient extraction and enrichment of target analytes from a variety of matrices. In this dissertation, two novel sol-gel coatings were developed for CME: (a) sol-gel benzyl-terminated dendrimer coating, and (b) sol-gel polytetrahydrofuran (poly-THF) coating. A detailed investigation was conducted to evaluate the performance of the newly developed sol-gel coatings in solvent-free extraction …

A Future With Hope: The Social Construction Of Hope, Help, And Dialogic Reconciliation In A Community Children's Mental Health System Of Care, Christine S. Davis

USF Tampa Graduate Theses and Dissertations

This research examines the social construction of hope in a community mental health system of care. Groopman (2004) defines hope as the elevating feeling we experience when we see a path to a better future. A year-long ethnographic study of a children's mental health system of care team found that members of the mental health care team construct hope for themselves and for the family they're helping by cycling through the dialectical tensions of hegemony and equality, marginalization and normalization, relating and othering, empowerment and disempowerment, and control and emotionality. They reconcile these tensions in dialogic moments of empathy toward …

Simulating The Electric Field Mediated Motion Of Ions And Molecules In Diverse Matrices, Joseph Hickey

USF Tampa Graduate Theses and Dissertations

Electroporation is a methodology for the introduction of drugs and genes into cells. This technique works by reducing the exclusionary nature of the cell membrane [125, 129, 186, 189]. Electroporation has successfully been used in electrochemotherapy and electrogenetherapy [57, 68, 86, 87, 110, 112, 131]. The two major components of electroporation are an induced transmembrane potential and the motion of the deliverable through a compromised cell membrane into the target cell [38, 55, 62, 114, 131]. These two components are both dependent on the electrophoretic motion of charged species in an applied electric field [45, 64, 75, 77, 177].

Currently, …

Integrated Uv-Vis Multiangle-Multiwavelength Spectrometer For Characterization Of Micron And Sub-Micron Size Particles, Yong-Rae Kim

USF Tampa Graduate Theses and Dissertations

Characterization of micron and sub-micron size particles requires the simultaneous measurement of the joint particle property distribution (JPPD). The JPPD is comprised of particle size, shape, orientation, composition, optical properties, and surface properties. Measurement of each of the particle properties independently is a difficult task and it has been only partially successful. To determine as many particle properties as possible using optical methods it is necessary to simultaneously measure all aspects of the interaction of the incident light with the particles of interest. This approach leads to the concept of multidimensional spectroscopy suggested by Prof. Garcia-Rubio. Dr. Bacon proved the …

Development Of A Generic Pda Based Control Mechanism For In-House Fabricated Miniature Sensors, Sunny Kedia

USF Tampa Graduate Theses and Dissertations

A novel method of controlling miniature sensors using Handspring Visor Prism PDA has been implemented. A generic motherboard was developed to map the data and address lines from the Visor onto a Complex Programmable Logic Device (CPLD) to provide basic electrical signals to the sensor board. The sensor board housed the sensor and contained application specific circuitry. The PDA, the motherboard, and the sensor board completed the control mechanism for the sensor. Miniature sensors and PDA based control mechanism scaled down the size of the complete system making the unit portable. This unit facilitated a faster analysis of data on …

On The Color Of The Orinoco River Plume, Ana L. Odriozola

USF Tampa Graduate Theses and Dissertations

In situ measurements were used to study the bio-optical properties of marine waters within the Gulf of Paria (GOP, Venezuela) and in the Southeastern Caribbean Sea (SEC) as they are affected by the seasonal discharge of the Orinoco River plume. The main purpose of this study was to determine the impact of colored dissolved organic matter (CDOM) (also known as Gelbstoff), phytoplankton, and total suspended matter (TSM) in the color of the Orinoco River plume. This information is essential for regional ocean color algorithms development.

Salinity and silica values indicate that the GOP and SEC waters were under the …

An Analysis Of Key Deer Herbivory On Forest Communities In The Lower Florida Keys, Mark Allan Barrett

USF Tampa Graduate Theses and Dissertations

The Key deer (Odocoileus virginianus clavium) population has increased from less than 80 individuals in the 1950s to approximately 700 by 2000. Over two-thirds of the Key deer population resides on two islands out of a potential 26 islands within their range. The skewed distribution resulted in high deer densities on Big Pine, No Name, and Big Munson keys. The objective of the study was to evaluate the effects of Key deer herbivory in mangrove, buttonwood, hammock and pineland habitats between islands with low, medium, and high densities of deer. Host-plant choice trials were conducted to determine Key …

They Shoot Single People, Don't They?, Dianne J. Smith

USF Tampa Graduate Theses and Dissertations

They Shoot Single People, Don't They? is a romantic comedy of errors set in Boston about Lexie, a twenty-five year old pediatric nurse with still perky breasts and lightly dimpled thighs who can figure out pediatric drug dosages, but is so severely relationship-challenged that she can't make any choice at all when it comes to men. Her life becomes a convoluted mess that includes two guys and a tangled web of lies.

After Marcus dumps her with a post-it note taped to her refrigerator door, Lexie thinks that her five-year plan to get married and have a baby are back …

Road Crack Condition Performance Modeling Using Recurrent Markov Chains And Artificial Neural Networks, Jidong Yang

USF Tampa Graduate Theses and Dissertations

Timely identification of undesirable pavement crack conditions has been a major task in pavement management. Up to date, myriads of pavement performance models have been developed for forecasting pavement crack condition with the traditional preferred techniques being the use of regression relationships developed from laboratory and/or field statistical data. However, it becomes difficult for regression techniques to predict the crack performance accurately and robustly in the presence of a variety of tributary factors, high nonlinearity, and uncertainty. With the advancement of modeling techniques, two innovative breeds of models, Artificial Neural Networks and Markov Chains, have drawn increasing attention from researchers …

Globalization, Ecotourism, And Development In The Monte Verde Zone, Costa Rica, Edgar Allan Amador

USF Tampa Graduate Theses and Dissertations

Ecotourism has been promoted globally as a model for sustainable development because it simultaneously benefits the environment and the residents of the given destination. However, many conservationists have questioned the long term sustainability of ecotourism as it is difficult to mitigate the impact of even low levels of tourism on a particular ecosystem. Further, social scientists including anthropologists have similarly questioned whether most residents of a particular destination actually benefit significantly from the alternative tourism economy.

The Globalization Research Center in cooperation with the Monteverde Institue in the Monte Verde Zone, Costa Rica, is undertaking a longitudinal study -- dubbed …

Lily’S Dilemma: Opposing Principles In The House Of Mirth, Debbie Lelekis

USF Tampa Graduate Theses and Dissertations

The focus of this study is Lily Bart and how she maneuvers in the cold, competitive world of upper class New York. To create a framework for my investigation, I draw upon naturalistic readings of the story which portray Lily as an outsider or "other" in her society. Lily's ethical principles lead to her destruction. Her marriage problem is just an example of her rejection of the life that her society expects her to lead. As she becomes more aware of a different philosophy of life--characterized by Selden's "republic of the spirit"--she finds it impossible to abide by the rules …

African American Women With Type 2 Diabetes: Understanding Self-Management, F Bridgett Rahim-Williams

USF Tampa Graduate Theses and Dissertations

Prescribed self-management behaviors have been found to be important factors affecting the rates of morbidity and mortality in multiple medical conditions including chronic diseases such as diabetes, a condition that disproportionately affects high health risk populations such as African Americans.

This study focused on understanding health behaviors, beliefs, and associated factors such as support systems and access to care that played a role in diabetes self-management and glucose control. The study also explored diabetes self-management education and its role in diabetes self-management. The research conducted in Pinellas and Hillsborough counties in Florida, included twenty-five African American women between the ages …

Chemical And Photolytic Degradation Of Polyacrylamides Used In Potable Water Treatment, Peiyao Cheng

USF Tampa Graduate Theses and Dissertations

Polyacrylamides (PAMs) are a class of polymers formed from acrylamide alone or copolymerized with other monomers. PAMs have been used in drinking water treatment as flocculants or coagulants in the highest volume among all the polymer types. In potable water treatment processes, polyacrylamides are often exposed to oxidants (e.g. chlorine and permanganate) and UV irradiation from sunlight or artificial sources. The purpose of this study is to evaluate the possible degradation of PAMs caused by chemical oxidation and/or UV irradiation.

Three types of PAM products (nonionic, cationic and anionic) were studied under free chlorine and permanganate oxidation, UV irradiation, and …

N-Thiolated Β-Lactams: Chemistry, Sar And Intracellular Target Of A Novel Class Of Antimicrobial And Anticancer Agents, Bart Allan Heldreth

USF Tampa Graduate Theses and Dissertations

N-Thiolated β-lactams (1) represent a promising new group of compounds with potent inhibition effects on bacteria, like Bacillus anthracis and methicillin resistant Staphylococcus aureus, and onco-systems, like breast cancer and leukemia. Originally developed as part of a synthetic pathway to bicyclic lactams, N-thiolated β-lactams have been shown in this laboratory to possess intriguing biological activities. The antibacterial activities of this new class of agents rely on novel structural features unlike those of any existing family of β-lactam drugs. The lactams seem to exert their effects intracellularly, requiring passage of the bioactive species through the cellular …

Mineral Solubilization From Municipal Solid Waste Combustion Residues: Implications For Landfill Leachate Collection Systems, Lisa R. Rhea

USF Tampa Graduate Theses and Dissertations

Leachate collection systems consist of a series of pipes installed beneath the waste at the base of a landfill. The liquid drains toward a central location where it is pumped and then treated, discharged, or recirculated. In some landfills, solid precipitates form in the collection system resulting in clogging and malfunctions of the drainage system. The formation of the precipitates is linked to the chemical and biological stability of the leachate generated within the landfill. To control the formation of precipitates and prevent clogging of leachate collection systems, it is important to understand factors that influence leachate characteristics.

Ashes from …

Development Of A Sensitive And Specific Biosensor Assay To Detect Vibrio Vulnificus In Estuarine Waters, Robert M. Ulrich

USF Tampa Graduate Theses and Dissertations

Biosensor development has the potential to meet the need for rapid, sensitive, and specific detection of pathogenic bacteria from natural sources. An antibody-based fiber-optic biosensor assay to detect low levels of Vibrio vulnificus in estuarine waters following an enrichment step was developed. The principle of the sensor is based on an immuno-sandwich assay where an anti-V. vulnificus polyclonal capture antibody preparation was first immobilized on a polystyrene fiber-optic waveguide using a biotin-avidin association. The capture antibody is responsible for binding the target cells to the waveguide. Cyanine-5-conjugated anti-V. vulnificus polyclonal antibodies are subsequently allowed to bind to immobilized …

Examining The Current U.S. Beef Trade Policies Concerning The Testing For Mad Cow Disease, David C. Miller

USF Tampa Graduate Theses and Dissertations

Despite existing mad cow disease surveillance efforts in the United States, in place since the 1980s, a cow that tested positive for mad cow disease was granted entrance into the U.S. in December, 2003. The cow that tested positive, according to witnesses, displayed no symptoms that are synonymous with advanced bovine spongiform encephalopathy, BSE. This occurrence had detrimental effects on the U.S. beef export market, as many countries banned American beef. Estimates of the damage inflicted reach into the billions of dollars. BSE in the U.S. has the potential of causing damages in other aspects as well. Aside from the …

Gait-Based Recognition At A Distance: Performance, Covariate Impact And Solutions, Zongyi Liu

USF Tampa Graduate Theses and Dissertations

It has been noticed for a long time that humans can identify others based on their biological movement from a distance. However, it is only recently that computer vision based gait biometrics has received much attention. In this dissertation, we perform a thorough study of gait recognition from a computer vision perspective. We first present a parameterless baseline recognition algorithm, which bases similarity on spatio-temporal correlation that emphasizes gait dynamics as well as gait shapes. Our experiments are performed with three popular gait databases: the USF/NIST HumanID Gait Challenge outdoor database with 122 subjects, the UMD outdoor database with 55 …

An Event Driven Single Game Solution For Resource Allocation In A Multi-Crisis Environment, Rashmi S. Shetty

USF Tampa Graduate Theses and Dissertations

The problem of resource allocation and management in the context of multiple crises occurring in an urban environment is challenging. In this thesis, the problem is formulated using game theory and a solution is developed based on the Nash equilibrium to optimize the allocation of resources to the different crisis events in a fair manner considering several constraints such as the availability of resources, the criticality of the events, the amount of resources requested etc. The proposed approach is targeted at managing small to medium level crisis events occurring simultaneously within a specific pre-defined perimeter with the resource allocation centers …

The Stress Response, Psychoeducational Interventions And Assisted Reproduction Technology Treatment Outcomes: A Meta-Analytic Review, Karen Rose Mumford

USF Tampa Graduate Theses and Dissertations

The psychological impacts of infertility have been well documented in the literature, providing evidence to support that at least some women who confront infertility are at risk for heightened distress and depressive symptoms. In response to this accumulated evidence, it has been argued that psychoeducational interventions may provide an important component to the treatment of infertility. While several theoretical models postulate the effects of stress on infertility treatment outcomes, results of these investigations have led to conflicting conclusions. However, a synthesis of the accumulated data examining the effects of stress on ART treatment outcomes was nonexistent until the conduct of …

Microglia Activation In Alzheimer's Disease, Kirk Phillip Townsend

USF Tampa Graduate Theses and Dissertations

The work detailed in this dissertation has an overarching theme of modulating microglia activation in both in vivo and in vitro models relevant to AD. The premise is that understanding microglia function in this context may lead to a better understanding of AD pathogenesis and thus to effective therapeutic interventions. In chapter 3, we employ a well-defined model of microglia activation whereby the intraperitoneal delivery of LPS results in CNS microglia activation and TNF-α production. Having previously identified that CD45 signaling pathways antagonized microglia TNF-α production in vitro and given that immunotherapy with anti-CD45 antibodies are already in clinical trials …

Isolation And Identification Of O-Linked-Β-N-Acetylglucosamine Modified Proteins (O-Glcnac) In The Developing Xenopus Laevis Oocyte, Sreelatha Paspuleti

USF Tampa Graduate Theses and Dissertations

Oocyte development in Xenopus laevis spans six morphologically distinct stages (stage I-VI), and is associated with a decrease in protein O-GlcNAc levels. As a first step in elucidating the role of O-GlcNAc in developing oocytes, initial efforts were focused on isolation and identification of fifteen modified proteins that decrease during oocyte development. Stage I oocytes due to their high amounts of these proteins, were used as starting material for purification. Multiple affinity and specific antibody based purification technique were initially used in an attempt to enrich the O-GlcNAc proteins. Due to the unique properties of the proteins …

Using Multiwavelength Uv-Visible Spectroscopy For The Characterization Of Red Blood Cells: An Investigation Of Hypochromism, Akihisa Nonoyama

USF Tampa Graduate Theses and Dissertations

Particle analysis using multiwavelength UV-visible spectroscopy provides the potential for extracting quantitative red blood cell information, such as hemoglobin concentration, cell size, and cell count. However, if there is a significant presence of hypochromism as a result of the concentrated hemoglobin (physiological value of 33%), successful quantification of red cell values would require a correction.

Hypochromism has been traditionally defined as a decrease in absorption relative to the values expected from the Beer-Lambert Law due to electronic interactions of chromophores residing in close proximity of one another. This phenomenon has been suggested to be present in macroscopic systems composed of …

Protecting Biscayne: An Analysis Of Strategies For The Protection Of Biscayne National Park, Janell M. Harvey

USF Tampa Graduate Theses and Dissertations

Biscayne National Park is located off the southeast coast of Florida and attracts approximately half a million visitors annually. Managers of Biscayne National Park are proposing a new General Management Plan (GMP) in order to update the recreational and commercial use of resources in the park. A Fishery Management Plan (FMP) is also being drafted simultaneously in conjunction with the Florida Fish and Wildlife Conservation Commission in order to address concerns associated with management of fish stocks within the park.

The proposed plan alternatives of the GMP and the recommendations of the FMP were developed in response to the negative …

Effects Of Participant Engagement On Alcohol Expectancies And Drinking Outcomes For A Computerized Expectancy Challenge Intervention, William Michael Hunt

USF Tampa Graduate Theses and Dissertations

The purpose of the present study was to examine the effect of varying the amount of participant engagement on alcohol expectancy and drinking outcomes during a social/sexual expectancy challenge based on Darkes and Goldman's (1993, 1998) protocol. This study was also intended to provide a test of the efficacy of administering an alcohol/placebo expectancy challenge outside of a live drinking scenario through video presented as part of a computerized intervention.

One hundred fifty-eight male participants across three sites were randomized into a no-intervention control group that received non alcohol-related information in a minimally interactive computerized format, a low-level engagement experimental …

Towards An E-Criture Feminine: Woolf, Duplessis, Cixous, And The Emerging Discursive Tradition In Women’S Online Diaries, Deborah Silverman Bowen

USF Tampa Graduate Theses and Dissertations

Women are drawing together the concepts of space, style,and medium and using these concepts collectively as a foundation for a new discursive tradition in the online autobiography. This dissertation, positioned in postmodern feminism, draws on a variety of disciplines to argue the development or evolution of a new women's discourse.

While a broad base of material exists which acknowledges the presence of women's discourse (formed by combining women's writing and women's genres), very little information explores its evolution, particularly in/on the new medium of the World Wide Web (WWW).

A combination of extant social and literary theories supports the idea …

A Vascular Plant Inventory Of Jay B. Starkey Wilderness Park, Pasco County, Florida, Emily Ferguson

USF Tampa Graduate Theses and Dissertations

Jay B. Starkey Wilderness Park, located in southwestern Pasco County, Florida, contains nearly 7,689.06 hectares (19,000 acres) and includes 18 natural communities. A floristic inventory was conducted on approximately 404.69 hectares (1,000 acres) within Jay B. Starkey Wilderness Park which included 11 community types. A comparison of those 11 communities in the study area with the rest of the park shows that the dominant community types do occur within the study site, making it representative of the entire park. The objective of this study conducted from May 2003 to October 2004 was to compile a list of the vascular plant …

Application Of Support Vector Machines And Neural Networks In Digital Mammography: A Comparative Study, Nivedita V. Candade

USF Tampa Graduate Theses and Dissertations

Microcalcification (MC) detection is an important component of breast cancer diagnosis. However, visual analysis of mammograms is a difficult task for radiologists. Computer Aided Diagnosis (CAD) technology helps in identifying lesions and assists the radiologist in making his final decision.

This work is a part of a CAD project carried out at the Imaging Science Research Division (ISRD), Digital Medical Imaging Program, Moffitt Cancer Research Center, Tampa, FL. A CAD system had been previously developed to perform the following tasks: (a) pre-processing, (b) segmentation and (c) feature extraction of mammogram images. Ten features covering spatial, and morphological domains were extracted …