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The Influence Of Obesity And Lifestyle Factors On Cognition In Middle-Aged And Older Adults, Andrew J. Fiscella

USF Tampa Graduate Theses and Dissertations

Obesity has been linked to conditions including cardiovascular disease, cancer, and diabetes. While the relationship of these conditions with obesity is well-established, the relationship between obesity and cognitive function is murkier.

Study 1 examined the association between being overweight or obese and performance on tests of executive function and episodic memory. The possibility that overweight/obesity affects cognition differently in middle vs. older age was also examined. Data from 6,012 participants in the 2018 wave of the Health and Retirement Study (HRS) were used. Body mass index (BMI) and waist circumference were used as overweight/obesity measures. Neither BMI (p = .809) …

“We Need To Have A Place To Vent And Get Our Frustrations Out”: Addressing The Needs Of Mothering Students In Higher Education Using A Positive Deviance Framework, Melissa León

USF Tampa Graduate Theses and Dissertations

This study examined the experiences of mothering students at four different colleges using a positive deviance (PD) framework. PD is an approach that seeks to identify positive behavioral patterns that help members of a community overcome structural barriers (Gross, et al. 2017). The Positive Deviance Framework was applied to investigate how some mothering students are successful in college and how their experiences could potentially help new or struggling mothering students. Eleven mothering students were interviewed to determine what interventions could assist mothering students who lack representation in the traditional college environment, a situation that often leads to feelings of isolation. …

Protein Folding Kinetics Analysis Using Fluorescence Spectroscopy, Dhanya Dhananjayan

USF Tampa Graduate Theses and Dissertations

The conformational changes that occur during the folding of a protein is an intensely researched area because of the impact that it has on human health and cellular functions. Protein stability is crucial in the context of protein misfolding and aggregation due to its implications on misfolding diseases such as amyloid fibril degenerative diseases (Alzheimer’s, Parkinson’s). By examining the kinetics of protein folding, we can gain valuable information about the folding mechanism and help us identify potential targets for many of the protein misfolding diseases.

In this study, we present the folding and unfolding kinetics of TEM-1 β-lactamase from Escherichia …

A Novel Role For Enos In Regulating Lymphatic Valve Development During Embryogenesis, Drishya Iyer

USF Tampa Graduate Theses and Dissertations

Lymphedema is a disease that occurs when lymph flow is impaired, resulting in tissue swelling, fibrosis, chronic inflammation and recurrent secondary infections. Lymphatic valves play a critical role in maintaining unidirectional lymph flow and evidence for valve defects have been reported in lymphedema patients. The lack of drugs that can correct lymphatic valve defects warrants a better understanding of the molecular regulators of lymphatic valve development and maintenance. Lymphatic valves first develop during embryogenesis in response to mechanotransduction signaling pathways triggered by oscillatory lymph flow. Since eNOS (gene name: Nos3) is a well characterized mechanotransduction signaling molecule in blood vessels, …

Contribution Of Contextual Factors And Neuropathology To Dementia, Monica E. Nelson

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that has extensive biological heterogeneity. It is not clear the extent to which this heterogeneity may be detected in participants without dementia, how it relates to incident AD dementia, and whether contextual factors may change how neuropathology relates to incident AD dementia. Therefore, this dissertation was completed using data from the Alzheimer’s Disease Neuroimaging Initiative (ADNI; n = 1,703) and the Czech Brain Aging Study (CBAS; n = 385) to address the following aims: to assess biological heterogeneity in participants without dementia, to relate this heterogeneity to incident AD dementia, and to …

Dynamical Polymer Chain Heterogeneities And Their Impacts On The Glass Transition, William Forrest Drayer

USF Tampa Graduate Theses and Dissertations

The utilization of polymeric materials has endured since prehistory, at first unknowinglyin the form of natural biopolymers such as wood and leather, culminating and continuing through the development of the plastics industry in the early twentieth century. Polymers, along with other materials including silicate glasses, readily exhibit the glass transition upon quenching: a continuous but rapid change from viscous (liquid) to elastic (solid) behavior. The physics surrounding the glass transition have been studied for over a century; while considerable progress has been made, there remains no universally accepted predictive theory of the glass transition. Modern development of glassy materials consists …

An Evaluation Of Measurement Invariance Of Dsm-5 Borderline Personality Disorder Criteria Across Heterosexual, Lesbian, Gay, And Bisexual Adults, E. Elisa Carsten

USF Tampa Graduate Theses and Dissertations

There is a robust, yet poorly understood relationship between non-heterosexual orientation and borderline personality disorder (BPD), with lesbian, gay, and bisexual individuals evidencing greater BPD symptoms compared to heterosexual individuals. Recent evidence suggests possible bias in BPD diagnostic criteria leading to greater endorsement among sexual minority individuals, which hinders researchers’ ability to make valid group comparisons. The present study utilized an epidemiological sample of 35,995 men and women to evaluate the extent of differential item functioning (DIF) among BPD criteria across sexual orientation groups using a multiple indicators multiple causes (MIMIC) approach. All criteria except affective instability and emptiness indicated …

Meeting Diverse Student Needs: An Examination Of A Physical Education Teacher Alumnus’ Culturally Responsive Teaching Beliefs And Their Enactment, Craigory V. Nieman

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine how one physical education teacher education (PETE) alumnus teaching in a CED urban school perceives culturally responsive teaching in physical education (PE), as well as how they enact it in their classes. Guided by culturally responsive teaching and self-efficacy, this study examined John, a physical education teacher education alumnus teaching in a CED urban elementary school. John completed the culturally responsive teaching self-efficacy scale (CRTSE; Siwatu, 2007), five semi-structured interviews, five reflection journal prompts, and eight voice memos over the course of 12 weeks. Additional data collection included text messages, emails, and …

Geoarchaeology Of Estuarine Seascapes In Tampa Bay, Florida., Kendal R. Jackson

USF Tampa Graduate Theses and Dissertations

This dissertation investigates the geological, ecological, and archaeological histories of Tampa Bay, Florida’s largest open-water estuary system. Previous geoscientific and archaeological work has studied the evolution of Florida’s estuaries as ‘pre-anthropogenic’ ecosystems and conceptualized Indigenous maritime societies as mostly passive inhabitants of dynamic ‘natural’ environments. Further, the environmental legacies of more recent, industrial-scale estuary modification attending Florida’s 20th-century population boom have been understudied—leading to popular and scientific confusion about historical conditions. The work composing this dissertation research seeks to reconceptualize Tampa Bay as a complex mosaic of cultural seascapes that have been variously altered and modified by human societies across …

Enhanced Methods In Forensic Mass Spectrometry For Targeted And Untargeted Drug Analysis, Dina M. Swanson

USF Tampa Graduate Theses and Dissertations

Forensic science has long used mass spectrometry for the analysis of drugsand metabolites. Traditionally, screening methods consisted of color tests or immunoassay and confirmation methods used gas chromatography with mass spectrometry, which was considered the gold standard for identification. In the last several decades, advances in liquid chromatography with mass spectrometry (LCMS) have brought this technology to the forefront of forensic drug identification. Drug identification in forensic drug chemistry and forensic toxicology has become increasingly challenging due to the rapid increase in illicit drug analogs including novel opioids such as fentanyl analogs. Traditional screening methodologies are often unable to identify …

An Experimental Study Of Negative Performance Feedback: Consideration Of A Cognitive Pathway And Individual Difference Factors, Ansley M. Bender

USF Tampa Graduate Theses and Dissertations

Negative performance feedback is relatively common and may be related to negative mental health outcomes, but there is a lack of sufficient experimental research. Further, little is known about the pathway by which effects emerge, or factors that may enhance or attenuate resilience to the effects of negative performance feedback. There is preliminary support for a cognitive pathway through appraisals of falling short of standards, but this specific model remains untested. Likewise, research suggests that perfectionism and positive future thinking may affect resilience in the context of negative performance feedback. The present study was among the first to experimentally test …

Stable Isotopic Investigation Of The Hydrological Cycle Of West-Central Florida, Toedsit Netratanawong

USF Tampa Graduate Theses and Dissertations

Stable isotopic compositions of oxygen, carbon, and hydrogen were used to identify the sources of water and of organic matter, pathway reactions, evaporation processes, and transfer mechanisms among various water types as well as the extent of water-rock reactions. An attempt to understand the isotopic compositions of the hydrological cycle of west-central Florida, their variations, baseline levels and controlling mechanisms constitutes parts of this dissertation.

A comparison of δ¹⁸O in rainwater (weighted mean = -4.4 o/oo) and surficial ground water (mean = -4.1 o/oo) indicates that rainwater recharges the surficial aquifer with only a minor isotopic change. δ …

Impact Of The Covid-19 Pandemic On Immigration-Related Stressors, Pregnancy, Birth, And Post-Partum Experiences Of Women Living Along The Us-Mexico Border, Isabela Solis

USF Tampa Graduate Theses and Dissertations

The COVID-19 pandemic coupled with immigration-related stressors had a profound effect on women who lived on the U.S.-Mexico border and their pregnancy, birth, and post-partum experiences. This project focused the experiences of 17 women living in El Paso, Texas and how border closure, economic insecurities, and family separation during the COVID-19 pandemic shaped women’s experiences. This research included interviewing maternal and child health experts to propose recommendations geared towards policy change. Finally, this project highlights the vast complexities that go into the pregnancy, birth, and post-partum period for women living on the U.S.-Mexico border, and how these experiences shape maternal …

Predicting Groundwater Spring Locations From Topographic And Climatic Data Using Maxent Modeling, Ayten Ece Koc

USF Tampa Graduate Theses and Dissertations

The World Resources Institute reveals that 17 countries face extremely high levels of water stress. Moreover, with increasing population and industrialization, the gap between water supply and demand increases day by day around the world. Groundwater is a key freshwater source, and springs are important resources as they enable to access groundwater. Therefore, it is crucial to monitor, protect, and manage groundwater springs. The first step in spring management is to recognize and define freshwater resources and to determine the locations of groundwater springs that serve as natural discharge points. Traditionally, field studies have been employed to determine the locations …

“They’Re Still Trying To Wrap Their Head Around Forever”: An Anatomy Of Hope For Spinal Cord Injury Patients, William A. Lucas

USF Tampa Graduate Theses and Dissertations

This dissertation draws on ethnographic data to investigate the nature of spinal cord injury (SCI) rehabilitation in Central Florida, using participant observation and interview data to understand how people with SCI (pwSCI) conceptualize their own disabilities after experiencing such radical alterations in their subjectivities. Using case studies and ethnographic vignettes, it argues that the extreme double binds in which pwSCI find themselves (where they are personally ordinarily disabled and socially extraordinarily novel; and where they are enabled resources to pursue “hopeful” therapy modalities while being designated as hopelessly disabled) is further polarized by the various legislative regimes of truth in …

Pamam- Cyclodextrin Conjugate Upregulates Brain-Derived Neurotropic Factor In Arpe-19 Cells, Gopika Ashokan

USF Tampa Graduate Theses and Dissertations

Background: Traumatic Brain Injury (TBI) is a major contributor to death and disability due to motorvehicle accidents, sports, physical abuse, and battlefield injuries. The primary insult to the brain leads to inflammation, vascular dysfunction, and oxidative stress in the brain as well as in the eye. This leads to loss of Retinal Ganglion Cells (RGCs) and downregulation of Brain derived Neurotopic Factor (BDNF). BDNF is a neurotrophic factor that binds to Tropomyosin Receptor Kinase B (TrkB) receptor to promote cell growth, survival, and differentiation. Current treatment strategies do not promote neuronal regeneration. Therefore, novel treatments are needed to restore vision …

Intimate Conversations: A Mixed-Methods Study Of African American Father-Adolescent Sexual Risk Communication, Shanda A. Vereen

USF Tampa Graduate Theses and Dissertations

Background: Approximately 50% of African American (AA) high school students are sexually active and a large proportion of sexually active AA students report engaging in sexual risk behaviors such as not using condoms at last intercourse. Consequently, AA adolescents in the United States experience outcomes of risky sexual behaviors at high rates. Evidence shows parents have an effect on shaping adolescent attitudes, values, and beliefs regarding sex through sexual risk communication (SRC). Although parent-adolescent SRC is associated with reductions in sexual risk behaviors among AA adolescents, research has primarily focused on mothers. While parents acknowledge that SRC is traditionally viewed …

Data-Driven Learning Algorithm Via Densely-Defined Multiplication Operators And Occupation Kernels., John Kyei

USF Tampa Graduate Theses and Dissertations

Consider a nonautonomous nonlinear evolution $\dot{x}=f(x,t,\mu)$, where the vector $x(t) \in \mathbb{R}^n$ represents the state of the dynamical system at time $t$, $\mu$ contains system parameters, and $f(\cdot)$ represents a dynamic constraint. In most practical applications, the nonlinear dynamic constraint $f$ is unknown analytically. The problem of approximating $f$ directly from data measurements generated by the system is a main goal of this manuscript. In the postulates of the Nonlinear Autoregressive (NAR) framework, we show that the problem of approximating $f$ can be studied through symbols of densely defined multiplication operators over a Reproducing Kernel Hilbert Spaces (RKHS). In this …

Factors To Consider For Effective Implementation Of Aac With Students With Autism: Saudi Special Education Teachers’ Perspectives, Mazen Abdurhman Almethen

USF Tampa Graduate Theses and Dissertations

This qualitative study aimed to explore the perceptions of Saudi special education teachers regarding teaching minimally verbal students with autism spectrum disorder (ASD) and factors facilitating the use of Augmentative and Alternative Communication (AAC) system in their classroom. Semi-structured interviews were conducted with five Saudi special education teachers. Teachers were from three schools at the General Directorate of Education in Unaizah, Saudi Arabia. The findings were analyzed by using thematic analysis. Findings revealed that Saudi special education teachers faced many challenges when teaching students with ASD who have minimal verbal abilities which includes expression difficulties, challenging behavior, and teacher-related challenges. …

Considerations For Curricular Reform In Undergraduate Chemistry: Cooperative Adoption Factors, Modeling Social Influence, And Focusing On Specific Populations, Jacob D. Mcalpin

USF Tampa Graduate Theses and Dissertations

This dissertation will go through the details of three projects highlighting considerations for curricular reforms in undergraduate chemistry. Improving the teaching of chemistry is facilitated by considering the problem from multiple directions, namely, faculty and students. In addition to the multiple perspectives, this dissertation also seeks to bring new methodologies into the spotlight to improve the toolbox for other chemistry or discipline-based education researchers. Additionally, this dissertation has shown in several locations how focusing in on subgroups within populations can reveal findings that would not have been apparent from a global average of the population. The three project describes in …

Training Parents Via Telehealth To Teach Manding To Children With Asd To Replace Problem Behavior, Sukarah Almulhim

USF Tampa Graduate Theses and Dissertations

This study assessed the effectiveness of using Behavioral Skills Training (BST) for parent training to implement functional behavior training and video modeling via a telehealth platform designed to increase manding abilities and to decrease problem behaviors with children with Autism Spectrum Disorders (ASD). With eight participants, this study used a multiple-baseline design across subjects (four parents and four children with ASD). The research was divided into three phases: parent training, baseline, and intervention. BST was administered remotely from the United States through synchronous video conferencing with families in different countries in the Middle East. All parents were able to learn …

The Local Neurologies Of Substance Use Triggers, Breanne I. Casper

USF Tampa Graduate Theses and Dissertations

Substance use triggers are largely understood to be embedded in drug use contexts and to be a major precipitator of relapse. Yet, the relationship between triggers and future instances of drug use is not quite clear– particularly in understanding the nuances of when triggers are “triggering” and when they are not. Additionally, there is little ethnographic work that explores how individuals interact with triggers in contexts related to substance use, particularly during periods of active use. This project employs ethnographic and survey research methods to interrogate substance use triggers in the harm reduction context. In doing so, it answers the …

A Forward Genetic Screen Identifies Genes Involved In Gametocyte Development Of The Human Malaria Parasite Plasmodium Falciparum., Jyotsna Chawla

USF Tampa Graduate Theses and Dissertations

Transmission of the deadly malaria parasite Plasmodium falciparum from human to mosquito is achieved by specialized intraerythrocytic sexual forms called gametocytes. Circulating in the vertebrate host, relatively few intraerythrocytic gametocytes are picked up during a bloodmeal to continue sexual development in the mosquito vector. Human-to-vector transmission thus represents an infection bottleneck in the parasite's life cycle for therapeutic interventions to prevent malaria. Though the crucial regulatory mechanisms leading to gametocyte commitment have recently come to light, networks of genes that control sexual development remain to be elucidated. Here, we report a forward genetic approach to identify genes associated with gametocyte …

Karl Marx On Human Flourishing And Proletarian Ethics, Sam Badger

USF Tampa Graduate Theses and Dissertations

This dissertation will show that Marx’s philosophy contains a notion of human “second nature” centered on the activity of labor with a corresponding class-centered theory of flourishing and emancipation. This notion shares important similarities with that of Aristotle but also differs in significant ways. Second nature for Marx is created and habituated through education and social labor. Moreover, human nature is molded into different forms as history progresses and modes and means of production change. In a class society everyone becomes is alienated from their nature in a way that inhibits their flourishing. This contrasts with an emancipated society, where …

Transformative Psychedelic Experiences At Music Events: Using Subjective Experience To Explore Chemosocial Assemblages Of Culture, Gabrielle R. Lehigh

USF Tampa Graduate Theses and Dissertations

Clinical interest in psychedelic treatments in the United States started in the 1950s, but anti-drug policy and anti-social sentiments quickly thwarted future research. The last decade has renewed clinical interest in using psychedelics to treat a diversity of mental health ailments. While these studies provide essential protocols, treatments, and therapy models for patients, they are limited in understanding the role of the contextual elements that influence psychedelic experiences and outcomes. This project examines how people use psychedelic substances outside medical settings by studying transformative psychedelic experiences at music events. This inquiry into psychedelic use utilizes an integrated framework of chemoethnography …

Self-Determination And The Lived Experience Of Employed Adults With Intellectual Disabilities: An Interpretative Phenomenological Analysis, Abdelrahman Mohammad Alzarie

USF Tampa Graduate Theses and Dissertations

Literature reveals that self-determination is a critical predictor of employment at the post-secondary level for individuals with Intellectual Disabilities (ID). However, current research concerning self-determination in the post-secondary work of individuals with ID is scarce and primarily focused on the perspectives of parents or educators or has been driven by evaluating learning models of self-determination. Furthermore, individuals with ID were not adequately represented as participants in the current research, and their voices were not given the opportunity to be heard. Therefore, this qualitative phenomenological study aimed to explore the lived experience of six Saudi employed adults with mild ID who …

Statistical Analysis Of Ribonucleotide Incorporation In Human Cells, Tejasvi Channagiri

USF Tampa Graduate Theses and Dissertations

During the DNA replication process, ribonucleotides, the building blocks of RNA, may be occasionally incorporated in the newly synthesized DNA. DNA is primarily composed of deoxyribonucleotides and there exist cellular mechanisms for removing ribonucleotides from DNA, which may point towards ribonucleotide incorporation being a replication error. Further, an excess of these ribonucleotides in the genome has been known to lead to genomic instability and has been implicated in human diseases. However, there are also hypotheses that suggest that ribonucleotides may be beneficial in certain circumstances. In this study we examine ribonucleotide incorporation in the human genome in several human cell …

Partial Characterization Of Plasmodium Falciparum Genes Implicated In Altered Artemisinin Stress Response, Caroline F. Simmons

USF Tampa Graduate Theses and Dissertations

Malaria is a parasitic illness caused by the Plasmodium spp., with an estimated 247 million malaria cases reported in 2021 across 84 endemic countries. Treatment of the deadliest malaria parasite, P. falciparum, relies on artemisinin combination therapies (ACTs). However, while ACTs have greatly decreased malaria mortality, this progress is threatened due to artemisinin (ART) partial resistance and ACT failure in Southeast Asia and Sub-Saharan Africa. SNPs to the Kelch13 (K13) gene are a well-characterized marker for ART resistance. However, increasing evidence that resistance to ART mediated by genes not related to K13 SNPs prompts the need to characterize other novel …

How Some Arboreal Salamanders (Genus Aneides) Jump, Glide, And Generate Lift, Christian E. Brown

USF Tampa Graduate Theses and Dissertations

The jumping and subsequent aerial behaviors of arboreal lungless salamanders (genus Aneides) had never been investigated or described. The aims of this research were to (1) compare jumping performance within the family Plethodontidae with a special focus on arboreal species from genus Aneides, (2) describe the biomechanics and kinematics of aerial behaviors after a jump or extended fall, and (3) model airflow over the morphology of the most aerially adept salamanders to investigate if they are capable of generating lift. First, I recorded salamanders jumping, locomoting vertically, dropping, and flying in a vertical wind tunnel. Then, I laser-scanned and reconstructed …

Organizations Ensuring Resilience: A Case Study Of Cortez, Florida, Karla Ariel Maddox

USF Tampa Graduate Theses and Dissertations

“Resilience” has often been defined by examining case studies in resilience failures. In contrast, this case study utilizes the oldest, still functional fishing village in Cortez, Florida to rhetorically analyze how organizational communicative practices have worked to ensure its resilience. Situating this conversation within Rhetoric proves valuable since so many attempts to define and utilize “resilience” seek to capitalize on its positive connotation but distort resilience definitions and practice. This dissertation explores three research questions: 1. “What systems and/or structures made our continued existence possible and what ideologies or goals drove their creation?” 2. “What ideologies, perceptions, and/or goals inspired …