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An Approach To Designing An Unmanned Helicopter Autopilot Using Genetic Algorithms And Simulated Annealing, Namir Aldawoodi

USF Tampa Graduate Theses and Dissertations

This dissertation investigates the application of Genetic Algorithms (GA) and Simulated Annealing (SA) based search techniques to the problem of deriving an auto-pilot that can emulate a human operator or other controller flying a Small unmanned Helicopter (SH). A Helicopter is a type of Vertical Take Off and Landing Vehicle (VTOL). The maneuvers are none aggressive, mild maneuvers, that include u-turns, ascending spirals and other none extreme flight paths.

The pilot of the helicopter is a Fuzzy logic Controller (FC) pilot; it is assumed that the pilot executes the maneuvers with skill and precision. The FC pilot is given set- …

The Evaluation Of A Commercially-Available Abduction Prevention Program, Kimberly V. Beck

USF Tampa Graduate Theses and Dissertations

Child abduction is a serious problem in the U.S.; therefore, it is essential that researchers evaluate the efficacy of currently available abduction prevention programs. This study evaluated the efficacy of a commercially-available abduction prevention program, The Safe Side. The participants included six 6-8-year old children with no prior abduction prevention training. A non-concurrent multiple baseline across participants design was used to evaluate the effects of the training. The participants' safety responses were assessed using in situ assessments within two different situations (responding to a knock on the door of the participant's home and interaction by a stranger in public) and …

The Study Of Compensatory Motions While Using A Transradial Prosthesis, Stephanie Lutton Carey

USF Tampa Graduate Theses and Dissertations

Improvement of prostheses requires knowledge of how the body adapts. A transradial prosthesis without a dynamic wrist component may cause awkward compensatory motion leading to fatigue, injury or rejection of the prosthesis. This work analyzed the movements of shoulder, elbow and torso during four tasks: drinking from a cup, opening a door, lifting a box and turning a steering wheel.

The main purpose of this study was to determine if using a basic transradial prosthesis that lacks motion of the forearm and wrist would cause significant compensatory motion of the shoulder, elbow and torso during the tasks. The second purpose …

The Association Between The Measles, Mumps, And Rubella Vaccine And The Development Of Autism: A Meta-Analysis, Rashad Carlton

USF Tampa Graduate Theses and Dissertations

Autism is a childhood developmental disorder characterized by impaired social interaction, difficulty with verbal and nonverbal communication and limited activities. The root cause of autism is unknown. However it has been postulated that administration of the measles, mumps, and rubella (MMR) vaccine may be causally related to the development of autism. MMR vaccination is a requirement for entry into schools, so any increase in adverse events associated with the vaccine carries widespread public health importance.

The primary objective of this study was to conduct a meta-analysis of the association between the MMR vaccination and the development of autism. The meta-analysis …

Natriuretic Peptides As A Humoral Link Between The Heart And The Gastrointetsinal System, Anteneh Addisu

USF Tampa Graduate Theses and Dissertations

Natriuretic peptides are a family of hormones released by several different tissues and exert various physiological functions by coupling with cell surface receptors and increasing intracellular cyclic gyanylyl monophosphate (cGMP). Atrial Natriuretic Peptide (ANP) and B-type Natriuretic Peptide (BNP) are released in response to mechanical stretch of the atrial or ventricular myocardium, respectively and their plasma level is markedly elevated during myocardial infarction and heart failure. Heart failure in turn is associated with symptoms suggestive of perturbed gastrointestinal function such as nausea, indigestion and malabsorption.

Intragastric pressure was monitored using a balloon catheter in anesthetized mice. The pressure before and …

A Constant Struggle: Renegotiating Identity In The Aftermath Of Rape, Jo Aine Clarke

USF Tampa Graduate Theses and Dissertations

The academic study of rape has historically ignored the recovery experience of the person being raped. Beyond medical and physical effects, and the possibility of legal prosecution, little attention has been paid. Existing research loses sight of the survivor's experience, ignoring the fact that a rape affects every aspect of life. The trauma is not only physical, but also impinges on the emotional, intellectual and interpersonal spheres. Rape can be, and often is, a life-threatening experience: one that needs to be faced and dealt with before there can be any sort of productive future. While it has been demonstrated that …

Functional Remodeling Of The Cardiac Glycome Throughout The Developing Myocardium, Marty L. Montpetit

USF Tampa Graduate Theses and Dissertations

Cell surfaces are replete with complex, biologically important glycans responsible for multiple cellular functions including cell adhesion and cellular communication. Proper protein glycosylation is essential for normal development and often pathologies are marked by altered glycosylation. Here, data showed that the auxillary subunit, ß₁, modified voltage-gated Na⁺ channel (Na_v) gating in an isoform-specific, sialic acid dependent, and saturating manner. The regulated activity of the hundreds of glycogenes (glycosylation-associated genes) is responsible for protein glycosylation; this could result in a glycome of thousands of glycan structures. Microarray analyses indicated that glycogene expression was highly regulated throughout …

Environmental Effects On Nano-Wear Of Gold And Kbr Single Crystal, Megan Pendergast

USF Tampa Graduate Theses and Dissertations

In order to successfully incorporate the tremendous possibilities of nanoscale applications into devices and manufacturing, significant studies need to be conducted of the properties and mechanics of materials of this small scale. In this thesis, the effect of repeated scanning of KBr, aluminum, and gold was studied by using a nanoindenter and Atomic Force Microscope (AFM) in varying environments. Additional research was performed to study the environmental effects of gold film scratching using a Taber Shear/Scratch Tester.

Scanning of KBr single crystal surface in air with a diamond tip in the Hysitron Triboindenter formed surface ripples 100 nm high, 1 …

A Modified Obesity Proneness Model In The Prediction Of Weight Status Among High School Students, Joyce E. Nickelson

USF Tampa Graduate Theses and Dissertations

The health and well-being of U.S. children is challenged by the immense crisis of obesity. The obesity proneness model, first described by Costanzo and Woody (1985), describes one mechanism by which parents influence obesity development. This model suggests that parents become concerned about their children's weight if their children show signs of becoming overweight and parents value weight highly. Parents communicate their concerns to their children and restrict their children's eating. Children internalize parents' concerns and become unable to regulate their eating. Hence, parents socialize children to be concerned about their weight but do not equip them to regulate eating, …

I Am Warrior Woman, Hear Me Roar: The Challenge And Reproduction Of Heteronormativity In Speculative Television Programs, Leisa Anne Clark

USF Tampa Graduate Theses and Dissertations

This paper explores how the "warrior woman" trope in western culture, as portrayed in late 20th century science fiction/fantasy and speculative television, reflects heteronormative/heterosexist discourses of femininity in American culture. First, I will examine feminine discourse in American culture, especially in the late 20th century. Then I will discuss how the tenets of second and third wave feminism influenced western paradigms of "the ideal female" and impacted pop culture by producing "warrior women" who both reflected and challenged heteronormative ideas and feminist principles. By examining several television shows produced in the United States and Great Britain from the late 1960s …

The Interrelatedness Of Homosexual Identity Development And Perceptions Of Campus Climate For Gay, Lesbian, Bisexual, And Transgender Students At The University Of South Florida, Tampa Campus, Frederic Drury Baker

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to determine the perceptions of campus climate at the University of South Florida, Tampa Campus for Gay, Lesbian, Bisexual, and Transgender students. Specifically, the study determined if there was any relationship between level of homosexual identity development acquired and perceptions of campus climate. If a relationship existed, it would influence the way that campus climate perceptions would be analyzed in future studies.

The population was the undergraduate student body at the University of South Florida taking at least six credit hours in the fall semester 2007. An online survey was created with two instruments …

A Potential Field Based Formation Control Methodology For Robot Swarms, Laura E. Barnes

USF Tampa Graduate Theses and Dissertations

A novel methodology is presented for organizing swarms of robots into a formation utilizing artificial potential fields generated from normal and sigmoid functions. These functions construct the surface which swarm members travel on, controlling the overall swarm geometry and the individual member spacing. Nonlinear limiting functions are defined to provide tighter swarm control by modifying and adjusting a set of control variables forcing the swarm to behave according to set constraints, formation and member spacing. The swarm function and limiting functions are combined to control swarm formation, orientation, and swarm movement as a whole. Parameters are chosen based on desired …

Opportunities For Spiritual Awakening And Growth In Mothering, Melissa J. Albee

USF Tampa Graduate Theses and Dissertations

My experiences as a mother have been enhanced by spirituality and my spirituality has been transformed through the practice of mothering. I will argue that part of the transformation available in mothering is that one can go from thinking of oneself as an individual with free will, self autonomy, and independence to believing maybe we are all more connected and dependent upon each other than we thought. I intend to explore my personal spiritual journey from an academic perspective in order to gain and share knowledge. Intense emotional experiences such as childbirth, learning how to take care of a person …

Multiple Modes Of Mdmx Regulation Affect P53 Activation, Daniele M. Gilkes

USF Tampa Graduate Theses and Dissertations

MDMX has emerged as a negative regulator of p53 transcriptional activity following DNA damage, loss of ribosomal integrity, and aberrant mitogenic signaling. Disruption of rRNA biogenesis by ribosomal stress activates p53 by releasing ribosomal proteins from nucleoli which bind MDM2 and inhibit p53 degradation. We found that p53 activation by ribosomal stress requires degradation of MDMX by MDM2. This occurs by L11 binding to the acidic domain of MDM2 which promotes its E3 ligase function preferentially towards MDMX. Further, unlike DNA damage which regulates MDMX stability through ATM-dependent phosphorylation events, ribosomal stress does not require MDMX phosphorylation suggesting p53 may …

Effects Of Electric Field On The Functions Of Cell Membrane Proteins, Zhongsheng Zhang

USF Tampa Graduate Theses and Dissertations

The most important and most common channels on cell membrane are voltage-gated Na+ and K+ channels. In so-called "excitable cells" like neurons and muscle cells, these channels open or close in response to changes in potential across the membrane in order to accomplish muscle contraction and transmit signals. By controlling the membrane potential, we observe extraordinary inactivation behaviors of the voltage-gated Na+ channels and the voltage-gated delayed rectifier K+ channels, which shows that electric stimulation pulses can temporarily close the Na+ and K+ channels, just as drugs, like tetrodotoxin (TTX) and tetraethylammonium (ETA), do.

The Na/K pump is essential for …

Interactions Of Neurons, Astrocytes And Microglia With Hucb Cell Populations In Stroke Models: Migration, Neuroprotection And Inflammation, Lixian Jiang

USF Tampa Graduate Theses and Dissertations

Previous studies demonstrated that intravenous administration of human umbilical cord blood (HUCB) cells could improve behavioral and neurological recovery of stroked animals following middle cerebral artery occlusion (MCAO). In addition, HUCB cell recipients had less of an inflammatory response with less leukocyte infiltration. In these studies we explored how HUCB cells change the inflammatory response of neurons, astrocytes, and microglia to hypoxia/ischemia. Initiation of the inflammatory response occurs with the expression of chemokines. We determined that monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein 1alph (MIP-1a), which are upregulated in the brain early after a stroke, induce migration of HUCB …

Drug Resistance To Topoisomerase Directed Chemotherapy In Human Multiple Myeloma, Joel G. Turner

USF Tampa Graduate Theses and Dissertations

Human multiple myeloma is an incurable hematological malignancy characterized by the proliferation of plasma cells in the bone marrow. Myeloma represents approximately 20% of all blood cancers. In this research we have explored examples of both intrinsic and acquired drug resistance in myeloma.

Topoisomerases are enzymes that are critical for cell division, especially in rapidly dividing cells such as are found in cancer. Topoisomerase poisons are a common group of drugs that are used to treat cancer. Topoisomerase I and II poisons used in the treatment of multiple myeloma include topotecan, mitoxantrone, doxorubicin, and etoposide

In order for topoisomerase drugs …

Designs And Applications Of Surface Acoustic Wave Sensors For Biological And Chemical Sensing And Sample Handling, Stefan Cular

USF Tampa Graduate Theses and Dissertations

Acoustic wave sensors have proven useful in many fields as primarily mass sensitive devices capable of responding to small environmental perturbations. The focus of this dissertation is the development of a new type of surface acoustic wave device with application to material property measurement, and biological and chemical sensing. This device is a combination of three independent acoustic wave devices with these waves propagated across the same area, while retaining independence of actuation and sensor function. The development of a complete sensor system, and its use and operation are presented for several example cases of chemical and biomarker sensing, and …

Gender, Quota Laws, And The Struggles Of Women’S Social Movements In Latin America, Merav Frazier

USF Tampa Graduate Theses and Dissertations

Assuming gender neutrality in comparative analysis, i.e. not including either explicit or implicit references to a particular gender or sex, runs the risk of camouflaging the unequal distribution of political power, economic influence, and political access for men and women. Unfortunately, in assuming such neutrality, one is blinded to the inherent flaws of political systems, the inequalities they create, and their lack of consideration of gender and women's rights. To counteract this inequality between the sexes, women's social movements are fighting to create gender awareness and establish formal policies that place them at the same level as their male counterparts, …

The Inflammatory Consequences Of Stress And Adiposity, Cathy A. Bykowski

USF Tampa Graduate Theses and Dissertations

The inflammatory process is important in protecting the body against the invasion of pathogens, but recent research has suggested that a long-term inflammatory response may lead to chronic diseases (e.g., Black, 2003; Wu, Dorn, Donahue, Sempos, & Trevisan, 2002). Two factors that have been implicated in the inflammatory and disease processes are stress and obesity (Black, 2003). While their individual lines of research continue to grow, few researchers have attempted to integrate these factors into one model to explain their effects on inflammation. This study aimed to replicate previous findings suggesting relationships between stress, obesity and inflammation and test an …

The Moderating Role Of Meaning And Defense Mechanisms In The Association Between Child Sexual Abuse And Romantic Relationship Dysfunction, Angela Fairweather

USF Tampa Graduate Theses and Dissertations

The current study investigated whether finding meaning in relation to sexual trauma and using mature defense mechanisms would moderate the association between child sexual abuse (CSA) severity and relationship and psychological adjustment in a sample of undergraduate women with a history of child sexual abuse. CSA severity was measured both objectively (i.e., severity of the abusive event) and subjectively (i.e., self-reported perceptions of the severity of the abusive event). As predicted, the interaction of objective CSA severity and mature defenses uniquely predicted one of four aspects of romantic relationship functioning (i.e., dyadic cohesion or doing joint activities with one's partner), …

The Interplay Of Economic, Climatic And Cultural Change Investigated Through Isotopic Analyses Of Bone Tissue: The Case Of Sardinia 4000-1900 Bc, Luca Lai

USF Tampa Graduate Theses and Dissertations

With the broader aim of reconstructing long-term resource use and ecological history for better policy making in times of environmental change, this study is an attempt to decode the mutual effects of human subsistence practices, climate and socio-cultural organization in Sardinia between 4000 and 1900 BC. Was economy changing due to climate change? Was the environment changing due to economic practices? And how were economic practices and socio-cultural factors interacting? The answer is complex, and some convergence of complex systems theory, historical ecology and agency supports this. Diet, at the interface of all of these as fulfilling biological needs constrained …

The Influence Of Anxiety And Depression On Cognitive Functioning In Parkinson’S Disease, Lynn E. Oelke

USF Tampa Graduate Theses and Dissertations

Depression and anxiety are common psychiatric disturbances in Parkinson's disease (PD). Past studies have demonstrated a relationship between depression and cognitive decline in PD; however, the unique influence of anxiety has not been well studied. The objective of the present study was to differentiate the unique influences of depression and anxiety on cognitive functioning in PD. Sixty-eight cognitively intact PD patients with mild to moderate motor disease severity completed self-report questionnaires and neuropsychological tests. Two hierarchical regression analyses were conducted with executive functioning performance as the criterion variable, and two additional hierarchical regression analyses were conducted with memory performance as …

Role Of Protein Kinase C-Iota In Prostate Cancer, Hla Yee Win

USF Tampa Graduate Theses and Dissertations

Prostate cancer is one of the leading causes of death among males in the United States. In this study, we hypothesized that an activated PKC-iota-dependent anti-apoptotic pathway, drives the cell cycle proliferation and survival of prostate cancer cells.

We investigated the role of atypical PKC-iota (PKC-ι) in androgen- independent prostate DU-145 carcinoma, androgen-dependent prostate LNCaP carcinoma compared to transformed non-malignant prostate RWPE-1 cells. Western blotting and immunoprecipitations demonstrated that PKC-ι is associated with cyclin-dependent activating kinase (CAK/Cdk7) in androgen-dependent, RWPE-1 and LNCaP cells but not in androgen-independent DU-145 cells. Treatment of prostate RWPE-1 cells with PKC-ι silencing RNA (siRNA) decreased …

Evolution And Field Application Of A Plankton Imaging System, Andrew Walker Remsen

USF Tampa Graduate Theses and Dissertations

Understanding the processes controlling the distribution and abundance of zooplankton has been a primary concern of oceanographers and has driven the development of numerous technologies to more accurately quantify these parameters. This study investigates the potential of a new plankton imaging sensor, the shadowed image particle profiling and evaluation recorder (SIPPER), that I helped develop at the University of South Florida, to address that concern. In the first chapter, results from the SIPPER are compared against concurrently sampling plankton nets and the optical plankton counter (OPC), the most widely used optical zooplankton sampling sensor in the field. It was found …

Analysis Of Glycerol 3-Phosphate Dehydrogenase And Sarco/Endoplasmic Reticulum Calcium Ion Channel Atpase For Use In Deep Metazoan Phylogenetics, Terry G. Campbell

USF Tampa Graduate Theses and Dissertations

Genetic sequence data have been widely used to analyze metazoan phylogenies for two decades, and numerous datasets have been generated for testing relationships at many different taxonomic levels. The rapid divergence of major metazoan body plans during the Cambrian explosion confounds the determination of phylogenetic relationships among metazoan phyla. Few molecular analyses at this level have used both multiple genes and broad taxonomic representation. Furthermore, most studies have relied exclusively on the small ribosomal subunit rRNA gene (SSU). Many of the other genes that have been examined have possessed substitution rates that are inappropriate for resolving the relationships among metazoan …

Representation And Learning For Sign Language Recognition, Sunita Nayak

USF Tampa Graduate Theses and Dissertations

While recognizing some kinds of human motion patterns requires detailed feature representation and tracking, many of them can be recognized using global features. The global configuration or structure of an object in a frame can be expressed as a probability density function constructed using relational attributes between low-level features, e.g. edge pixels that are extracted from the regions of interest. The probability density changes with motion, tracing a trajectory in the latent space of distributions, which we call the configuration space. These trajectories can then be used for recognition using standard techniques such as dynamic time warping. Can these frame-wise …

Transition Experiences Of Selected Emerging Adults With Emotional And Behavioral Difficulties In Higher Education, Kathleen M. Fowler

USF Tampa Graduate Theses and Dissertations

Transitioning into adulthood is an exciting and challenging time for emerging adults, and is even more difficult for young adults with emotional and behavioral difficulties (EBD). Even though the number of transition-aged individuals with EBD is significant, few studies have explored the experiences of this population engaged in educational activities, and there is limited information regarding the personal experiences of emerging adults with EBD. The purpose of the study is to acquire a better understanding of the experiences of young adults diagnosed with an EBD who are working toward their academic goals. Further, this study may give voice to individuals …

A Novel Biostable 3d Porous Collagen Scaffold For Implantable Biosensor, Young Min Ju

USF Tampa Graduate Theses and Dissertations

Diabetes is a chronic metabolic disorder whereby the body loses its ability to maintain normal glucose levels. Despite of development of implantable glucose sensors in long periods, none of the biosensors are capable of continuously monitoring glucose levels during long-term implantation reliably. Progressive loss of sensor function occurs due in part to biofouling and to the consequences of a foreign body response such as inflammation, fibrosis, and loss of vasculature.

In order to improve the function and lifetime of implantable glucose sensors, a new 3D porous and bio-stable collagen scaffold has been developed to improve the biocompatibility of implantable glucose …

Synthesis Of Novel Cysteine Peptide Nucleic Acid (Cpna), Mehul J. Ajmera

USF Tampa Graduate Theses and Dissertations

Many diseases are caused due to abnormalities in production of specific protein. Across this protein the conventional lock and key mechanism shows binding at the specific cites of protein. However use of antisense technology can prevent formation of protein. It does so by binding to mRNA and prevents transcription. The structural modifications lead to synthetic molecules with 18-mer units which show significant improvement in binding properties, this gives birth to a new class of oligomers called Peptide Nucleic Acid (PNA). We herein report cysteine based PNA called CPNA.