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Marginalized African American Grandmothers Raising Their School-Aged Grandsons: Perspectives On Parental Involvement, Felita Grant Lott

USF Tampa Graduate Theses and Dissertations

Literature suggests strong, positive and mutual relationships between home and school lead to higher levels of success and achievement for students. Schools should begin to focus on being more aware and sensitive to the cultural and social wealth that marginalized families carry. When institutions of learning begin to tap into and embrace the strengths of their families, it is likely to be of mutual benefit for the students, staff and families. Utilizing qualitative research methodology, this study sought to understand African American grandmothers' perspectives on parental involvement, and identify strategies that supported, maintained, and facilitated their involvement in the educational …

Refinement, Application, And Evaluation Of Cognitive And Affective Chemistry Measures For College Students, Keily Heredia

USF Tampa Graduate Theses and Dissertations

This work describes three case studies conducted to address two major problems in the area of chemistry education research, the lack of reported psychometrics regarding instrument scores, and the need for well-characterized assessments to evaluate college chemistry curricula.

The first case study describes a psychometric evaluation of the Colorado Learning Attitudes about Science Survey (CLASS), an instrument designed to assess student beliefs about the learning of chemistry. Results from this work suggest that the CLASS instrument provides fertile ground for short instruments with reasonable psychometric properties. Responses to a single scale instrument, created from CLASS, showed that students in an …

Ecological Diversity In Hillsborough County, Florida: Correlations Between Landscape Metrics And Socio-Demographic Variables, David Godfrey

USF Tampa Graduate Theses and Dissertations

Landscape metrics, a means of quantifying landscape attributes, are frequently used in landscape ecology to describe the spatial characteristics of a landscape, but they have been less often used in anthropology. Using geographic information system (GIS) software, this study tests a method that investigates statistical correlations between groundcover landscape metrics and socio-demographic variables in Hillsborough County, Florida. Statistically significant correlations were found, illustrating the potential utility of this exploratory method. Wealthier areas with fewer ethnic minorities tend to be more fragmented and diverse in terms of groundcover; these areas also tend to have a lower percentage of impervious surfaces. The …

Assessment Of A Modified Double Agar Layer Method To Detect Bacteriophage For Assessing The Potential Of Wastewater Reuse In Rural Bolivia, Sakira N. Hadley

USF Tampa Graduate Theses and Dissertations

Water scarcity is a global concern that impacts many developing countries, forcing people to depend on unclean water sources for domestic, agricultural, and industrial needs. Wastewater is an alternative water source that contains nutrients needed for crop growth. Wastewater reuse for agriculture can cause public health problems because of human exposure to pathogens. Pathogen monitoring is essential to evaluate the compliance of wastewater with established World Health Organization (WHO) and U.S. Environmental Protection Agency (EPA) wastewater reuse guidelines. Indicator organisms are commonly used to detect pathogens in water and wastewater because they are quick and easy to measure, non-pathogenic, and …

Risk And Hiv-Serodiscordant Couples In Porto Alegre, Brazil: "Normal" Life And The Semantic Quarantine, Shana Hughes

USF Tampa Graduate Theses and Dissertations

The objective of this research was to develop a holistic understanding of how risk, especially the risk of HIV transmission, is constructed and negotiated in the daily lives of a group of heterosexual, HIV-serodiscordant couples in Porto Alegre, Brazil. Couples serodiscordant for HIV are those in which one partner is infected and the other is not. Data were gathered through participant observation and semi-structured interviews with serodiscordant couples, as well as key informants in HIV/AIDS-related civil society, government, and biomedical practitioners in Porto Alegre. Interviews were recorded and transcribed and relevant study materials were coded and subjected to thematic and …

Evaluation Of Four Portable Cooling Vests For Workers Wearing Gas Extraction Coveralls In Hot Environments, Joseph Kevin Johnson

USF Tampa Graduate Theses and Dissertations

Excessive exposure to heat stress can cause a host of heat-related illnesses. For laborers, job specific work demands and protective garments greatly increase the risk of succumbing to the effects of heat stress. Microclimate cooling has been used to control heat stress exposure where administrative or engineering controls are not adequate. This study tested the performance of four personal cooling vests for use with insulated protective clothing (gas extraction coveralls) in warm-humid (35 ° C, 50% relative humidity) and hot-dry (40°C, 30% relative humidity) conditions. On 10 separate occasions, 5 male volunteers walked on a treadmill to elicit a target …

The Effects Of Scaling On Bite Force And Suction Index In The Eastern Hellbender (Cryptobranchus Alleganiensis), Nicholas Patrick Larghi

USF Tampa Graduate Theses and Dissertations

The hellbender (Cryptobranchus alleganiensis) is a salamander that grows over a large range of body sizes (2-74 cm total length) making it an ideal organism for examining the effects of body size on morphology and performance. The goal of this study is to investigate the morphology changes over ontogeny and change in

feeding ability. Cryptobranchus feeds on small aquatic insects as juveniles and shifts to crayfish as they get larger. Morphology can be expected to change as an organism grows larger, and because morphology and performance are closely linked, this morphological change can result in a change in …

Chiral Phosphoric Acid-Catalyzed Acetalization And Iso-Pictet-Spengler Reactions, Matthew Jon Kaplan

USF Tampa Graduate Theses and Dissertations

The development of novel asymmetric reaction methodologies has been invaluable in both the academic and industrial world. In just 15 years, organocatalysis has provided a new means of developing asymmetric reaction methodologies using catalysts that are environmentally benign, relatively inexpensive, bench stable, and non-toxic. One development in organocatalysis that is important to our group in particular is chiral phosphoric acid-catalysis. BINOL-derived and VAPOL-derived phosphoric acids have proven to be excellent catalysts for a number of reactions.

The two projects I will discuss my efforts on are acetalization and iso-Pictet-Spengler reactions. These were projects that I performed during my first two …

Effect Of Solids Retention Time On The Denitrification Potential Of Anaerobically Digested Swine Waste, Maureen Njoki Kinyua

USF Tampa Graduate Theses and Dissertations

Three continuously stirred tank reactors (CSTR) were operated in semi continuous mode treating swine waste using anaerobic digestion. The reactors were used to test the effect of solid retention time (SRT) on CH4 yield, total ammonia nitrogen (TAN) concentrations, % volatile solids (VS), chemical oxygen demand (COD) and volatile fatty acids (VFA) removal, readily biodegradable COD concentration and the denitrification potential for the effluent in a biological nutrient removal (BNR) system. During Phase I of the study, the three reactors were operated at the same 28 day SRT for 16 weeks. SRTs were then changed during the 12 week Phase …

Associations Of Perceived Stress, Sleep, And Human Papillomavirus In A Prospective Cohort Of Men, Stephanie Kay Kolar

USF Tampa Graduate Theses and Dissertations

Introduction: Mucosal human papillomavirus (HPV) infection is the most common sexually transmitted infection (STI) and is associated with genitals warts, anogenital cancers, and oropharyngeal cancers. Most sexually active persons will become infected with HPV at some point in their lives, however few will develop HPV-related diseases such as warts, lesions, or cancer as a result of the infection. It is unclear why a minority of individuals fail to clear HPV infection and develop clinical disease. Due to initial associations with cervical lesions, much research has focused on women. Th1 type immune responses have been associated with successful response to HPV …

Reaction Enthalpy And Volume Profiles For Excited State Reactions Involving Electron Transfer And Proton-Coupled Electron Transfer, William Antonio Maza

USF Tampa Graduate Theses and Dissertations

Electron transfer, ET, and proton-coupled electron transfer, PCET, reactions are central to biological reactions involving catalysis, energy conversion and energy storage. The movement of electrons and protons in either a sequential or concerted manner are coupled in a series of elementary reaction steps in respiration and photosynthesis to harvest and convert energy consumed in foodstuffs or by absorption of light into high energy chemi-cal bonds in the form of ATP. These electron transfer processes may be modulated by conformational dynamics within the protein matrix or at the protein-protein interface, the energetics of which are still not well understood. Photoacoustic calorimetry …

Eschatology In A Secular Age: An Examination Of The Use Of Eschatology In The Philosophies Of Heidegger, Berdyaev And Blumenberg, John R. Lup, Jr.

USF Tampa Graduate Theses and Dissertations

The topic of eschatology is generally confined to the field of theology. However, the subject has influenced many other fields, such as politics and history. This dissertation examines the question why eschatology remained a topic of discussion within twentieth century philosophy. Concepts associated with eschatology, such as the end of time and the hope of a utopian age to come, remained largely background assumptions among intellectuals in the modern age. Martin Heidegger, Nicolai Berdyaev, and Hans Blumenberg, however, explicitly addressed the subject in their philosophies. The impetus of this study is Heidegger's statement, "Being itself is inherently eschatological," which indicates …

A Child Distracted: Understanding The Relationship Between Pediatric Obsessive-Compulsive Disorder And Subjective Well-Being, Joshua M. Nadeau

USF Tampa Graduate Theses and Dissertations

Obsessive Compulsive Disorder (OCD) is a commonly diagnosed mental health condition among children and adolescents, with studies suggesting that OCD has the potential for significant disruption of academic and social performance. Subjective well-being (SWB) represents a non-traditional conceptualization of mental health within the dual factor model, wherein SWB and measures of psychopathology (e.g., problematic levels of internalizing and externalizing behaviors) provide a more comprehensive picture of mental wellness. The current study examined the nature of the relationship between clinical characteristics of pediatric OCD and SWB within school-age youth (N=65) seeking treatment from an outpatient pediatric neuropsychiatric clinic. Additionally, the potential …

Excellent Leadership In Public Relations: A Question Of Experience And The Pr Practitioner, Roelof Oldenbanning

USF Tampa Graduate Theses and Dissertations

To better understand excellent leadership in public relations (PR) of developing and experienced PR practitioners, this study focuses on the important qualities and dimensions of excellent leadership practitioners. This study continues on the work of Meng, Berger, Gower, and Heyman (2012) in their attempt to advance understanding of leadership in the PR field.

According to the study's participants (N = 72): strategic decision-making capability, problem-solving ability, and communication knowledge and expertise are the three most important qualities of excellent leadership. Moreover, respondents rated that communication skills training, individual initiative and desire, and on-the-job experiences lead to excellent PR leadership. About …

Framing Colombian Women's Beliefs, Values And Attitude Towards Sex And Sexual High-Risk Behaviors, Rosa Ore

USF Tampa Graduate Theses and Dissertations

Hispanic immigrants constitute the largest and fastest growing groups of minorities in the United States. According to the 2010 Census, there are 50.5 million Hispanics in the United States, making up 16.3% of the total population (Passel, Cohn & Lopez, 2011). Furthermore, the state of Florida is home to 4,223,806 Hispanics (U.S. Census Bureau, 2010). Because the Hispanic population continues to grow, it is important to study their sexual health behaviors because diseases linked to risky sexual behaviors account for approximately 20,000 U.S. deaths each year, and are linked to a number of adverse reproductive outcomes (Abraido-Lanza, Chao, & Florez, …

Ecological Interactions Influencing Avicennia Germinans Propagule Dispersal And Seedling Establishment At Mangrove-Saltmarsh Boundaries, Jennifer Mcclain Peterson

USF Tampa Graduate Theses and Dissertations

Mangroves and saltmarshes are ecologically important coastal ecosystems; unfortunately, these low-lying coastal ecosystems are vulnerable to global climate change. As sea-levels rise, mangroves are expected to shift their distribution landward towards higher elevation sites that are occupied by other plants, including saltmarsh taxa. Therefore, mangrove recruits at the leading edge of expansion may interact with diverse assemblages of saltmarsh plants, and these interactions could influence the success of mangrove encroachment into higher tidal-elevation areas. The purpose of the research presented here was to investigate empirically the ecological interactions that may influence the recruitment of the black mangrove, Avicennia germinans, …

Analytical Modeling, Perturbation Analysis And Experimental Characterization Of Guided Surface Acoustic Wave Sensors, Onursal Onen

USF Tampa Graduate Theses and Dissertations

In this dissertation, guided surface acoustic wave sensors were investigated theoretically and experimentally in detail for immunosensing applications. Shear horizontal polarized guided surface acoustic wave propagation for mass loading sensing applications was modeled using analytical modeling and characterized by perturbation analysis. The model verification was performed experimentally and a surface acoustic wave immunosensor case study was presented. The results of the immunosensing were also investigated using the perturbation analysis.

Guided surface acoustic wave propagation problem was investigated in detail for gravimetric (or mass loading) guided wave sensors, more specifically for immunosensors. The analytical model was developed for multilayer systems taking …

Biochemical Aspects Of The Thermal Sensitivity And Energy Balance Of Polar, Tropical And Subtropical Teleosts, Eloy Martinez

USF Tampa Graduate Theses and Dissertations

The maintenance of a functional energy balance in ectothermic fauna could be challenging in a thermally disparate environment. Biochemical adaptations at the enzyme and membrane levels allows for a set compensatory mechanism that allow the individual to maintain an energetic surplus, thus allocating energy for growth and reproduction. The present work describes how the energetic machinery in the cell, particularly the mitochondrion, could be affected by temperature changes. More specifically, this work aimed to determine how environmental temperature affects the mitochondria energetic performance of fishes from disparate thermal regimes.

Mitochondrial ATP production efficiency was evaluated in fishes from polar, tropical …

Investigation Of Hsf1 Interacting Partners Via A Genome-Wide Yeast Two-Hybrid Screen, Jamie Elizabeth Mendez

USF Tampa Graduate Theses and Dissertations

Heat shock factor 1 (HSF1) is the master transcriptional regulator of the heat shock response (HSR), an evolutionarily conserved cellular stress response. HSF1 promotes the expression of a variety of molecular chaperones that aid in restoring protein homeostasis upon exposure to proteoxic stress. However, all of the proteins responsible for regulating the HSR together with HSF1 are unknown. A genome-wide yeast two hybrid screen was performed to identify new S. cerevisiae Hsf1 protein interacting partners. Two GAL4 DNA binding domain-Hsf1 fusion proteins (baits) were constructed with mutations in the Hsf1 C-terminal activation domain to dampen Hsf1 mediated auto-activation of the …

Gradients In Season, Latitude, And Sea Ice: Their Effect On Metabolism And Stable Isotopic Composition Of Antarctic Micronekton, Erica H. Ombres

USF Tampa Graduate Theses and Dissertations

Respiration, metabolic enzyme assays, and body composition parameters were measured in the Antarctic krill Euphausia superba during the summer, fall and winter on the Western Antarctic Peninsula (WAP). E. superba of all sizes decrease their metabolism from the summer to the winter. These same parameters were also measured along the WAP during the austral fall 2010. E. superba's enzyme activity indicated that there was a latitudinal gradient to the decline in metabolism along the WAP with the more northerly sites having significantly higher metabolic enzyme activities than the sites to the south.

Carbon (δ¹³C) and nitrogen (δ^{15 …}

Functional And Mutational Analysis Of Human Recq-Like Dna Helicases In Saccharomyces Cerevisiae, Hamed Mirzaei-Souderjani

USF Tampa Graduate Theses and Dissertations

RecQ-like proteins are a family of DNA helicases that are evolutionary conserved from prokaryotes to eukaryotes. A large amount of experimental evidence suggests these proteins have a major role in the maintenance of genome stability. In humans five RecQ like helicase have been identified (RecQL1, BLM, WRN, RecQL4, and RecQL5), three of which are associated with rare genetic disorders with sever chromosomal and developmental abnormalities, and an elevated predisposition to cancer. Among the disease associated RecQ-like helicases, BLM and WRN have been subject to extensive research, while our collective knowledge about the function of RecQL4 is still very limited. Similarly, …

Light-Environment Controls And Basal Resource Use Of Planktonic And Benthic Primary Production, Kara Radabaugh

USF Tampa Graduate Theses and Dissertations

Consumers in marine and estuarine environments have a strong reliance on planktonic and benthic primary production. These two basal resources form the foundation of aquatic food webs, yet the abundance of phytoplankton and benthic algae are frequently inversely related due to competition for light and nutrients. As a result, optimal habitats for benthic and planktonic consumers vary spatially and temporally. To investigate these trends, three studies were conducted focusing on light attenuation and basal resources in a bay, river, and on a continental shelf.

δ¹³C and δ¹⁵N stable isotopes can be used as endogenous tracers to …

Do Mathematics And Reading Competencies Integrated Into Career And Technical Education Courses Improve High School Student State Assessment Scores, Kristin Bowles Pierce

USF Tampa Graduate Theses and Dissertations

A quasi experimental study tested a contextual teaching and learning (CTL) model for integrating reading and mathematics competencies through 13 introductory Career and Technical Education (CTE) courses. Volunteer CTE Lead Teachers with assistance from academic teachers, developed integrated units. The purpose of this study was to determine whether students who participated in CTE courses that integrated core mathematics and reading standards performed better on a test of mathematics and reading skills compared to students who participated in traditional, non-integrated courses. The treatment group consisted of students in the 13 introductory courses taught by the CTE Lead Teachers and the control …

The Baby Blues: Mothers' Experiences After Adoption, Brigette Barno Schupay

USF Tampa Graduate Theses and Dissertations

ABSTRACT

It is not uncommon for new mothers to experience depression. However, depression after the arrival of a child is not limited to biological mothers. The term Post-Adoption Depression Syndrome (PADS) was created to capture the unique type of depression that may occur after adopting a child. The nature and prevalence of depression after adoption is still largely unknown since there is little published research exploring the experiences of mothers in the first year after adopting a child. The characteristics of this disorder are reportedly comparable to postpartum depression in a biological mother. A review of postpartum literature reveals risk …

High School Students In College-Level Classes: Associations Between Engagement, Achievement, And Mental Health, Rachel Anne Roth

USF Tampa Graduate Theses and Dissertations

Student engagement is a multifaceted construct gaining increased interest within the fields of psychology and education. Current literature suggests that student engagement is linked to important student outcomes including academic achievement, psychopathology, and mental wellness; however, there is a dearth of studies that have examined all components of student engagement simultaneously as they relate to the aforementioned outcomes. Additionally, past literature has found support for a decreasing trend in student engagement across the school years, but less attention has been paid to student engagement in the high school years. Among high school students, a particular subgroup has been virtually ignored: …

Sirt1 Regulation Of The Heat Shock Response In An Hsf1-Dependent Manner And The Impact Of Caloric Restriction, Rachel Rene Raynes

USF Tampa Graduate Theses and Dissertations

The heat shock response (HSR) is the cell's molecular reaction to protein damaging stress and is critical in the management of denatured proteins. Activation of HSF1, the master transcriptional regulator of the HSR, results in the induction of molecular chaperones called heat shock proteins (HSPs). Transcription of hsp genes is promoted by the hyperphosphorylation of HSF1, while the attenuation of the HSR is regulated by a dual mechanism involving negative feedback inhibition from HSPs and acetylation at a critical lysine residue within the DNA binding domain of HSF1, which results in a loss of affinity for DNA. SIRT1 is a …

Utilizing Habit Reversal And Contingency Contracting To Impact Eating Habits With Obese Individuals, Christopher Thomas Scull

USF Tampa Graduate Theses and Dissertations

One of the most difficult set of behaviors for individuals to engage in is weight management. The United States boasts an astonishing 35% obesity rate in adults while children show almost a 20% obesity rate. These statistics identify the importance of developing effective methods to help change an individual's eating habits. There are many eating habits that may contribute to an individual gaining or losing weight over a period of time. Simplified habit reversal (SHR) has an impressive history in shaping desirable responses to compete with many different types of habitual behaviors. The present study extended the SHR procedure to …

Direct And Indirect Effects Of Agrochemicals On Bacterial Pathogens And Fecal Indicator Bacteria, Zachery Staley

USF Tampa Graduate Theses and Dissertations

The presence of agrochemical residues in both urban and agricultural water bodies has become ubiquitous, often producing deleterious effects in the impacted watershed including reductions in biodiversity, alterations in species interactions, and toxicity to non-target organisms. While these effects have been studied on metazoan consumers, the consequences of agrochemical contamination on microorganisms, such as bacteria, protozoa, and viruses, are poorly understood. Agrochemicals could act directly on microorganisms, including pathogens, by either facilitating their survival or decreasing their abundance. Further, a multitude of indirect effects of agrochemicals on microorganisms are possible, whereby agrochemicals alter predation, competition, or parasitism on or available …

Synthesis And Properties Of Polymer Nanocomposites With Tunable Electromagnetic Response, Kristen Lee Stojak

USF Tampa Graduate Theses and Dissertations

Multifunctional polymer nanocomposites (PNCs) are attractive for the design of tunable RF and microwave components such as flexible electronics, attenuators, and antennas due to cost-effectiveness and durability of polymeric matrices. In this work, three separate PNCs were synthesized. Magnetite (Fe3O4) and cobalt ferrite (CFO) nanoparticles, synthesized by thermal decomposition, were used as PNC fillers. Polymers used in this work were a commercial polymer provided by the Rogers Corporation (RP) and polyvinylidene fluoride (PVDF). PNCs in this thesis consist of Fe3O4 in RP, CFO in RP, and Fe3O4 in PVDF. Characterization techniques for determining morphology of the nanoparticles, and their resulting …

Regulation Of The Tumor Suppresser P53 And Survivin By Ras And Ral Gtpases:Implications For Malignant Transformation, Awet G. Tecleab

USF Tampa Graduate Theses and Dissertations

Abstract

Although the critical role of the small GTPases Ras and Ral in oncogenesis has been well documented, much remains to be investigated about the molecular mechanism by which these GTPases regulate malignant transformation. The work under this thesis made two major contributions to this field. The first is the discovery that K-Ras, RalA and/or RalB are required for the maintenance of the high levels of the anti-apoptotic protein survivin in some human cancer cells, and the second is the demonstration that down regulation of K-Ras, RalA and/or RalB, but not Raf-1 or Akt1/2, stabilizes the tumor suppressor p53 and …