Digital Commons Network^™

Region Of Interest Based Compression Of Grayscale Images, Adhokshaja Achar Budihal Prasad

USF Tampa Graduate Theses and Dissertations

Image compression based on Region of Interest (ROI) has been one of the hot topics of interest in image processing. There is not a single widely accepted method for detecting the ROI automatically form an image. To reduce the transmission bandwidth and storage space requirements of gray scale images, an algorithm is suggested for detecting the ROI automatically based on Tsallis entropy method.

Tsallis entropy method is used to segment the image into two segments, the ROI and the background. These two segments can then be compressed at different rates, to avoid losing information in the ROI while achieving a …

Patient Populations, Clinical Associations, And System Efficiency In Healthcare Delivery System, Yazhuo Liu

USF Tampa Graduate Theses and Dissertations

The efforts to improve health care delivery usually involve studies and analysis of patient populations and healthcare systems. In this dissertation, I present the research conducted in the following areas: identifying patient groups, improving treatments for specific conditions by using statistical as well as data mining techniques, and developing new operation research models to increase system efficiency from the health institutes’ perspective. The results provide better understanding of high risk patient groups, more accuracy in detecting disease’ correlations and practical scheduling tools that consider uncertain operation durations and real-life constraints.

Improvements On Scientific System Analysis, Vladimir Grupchev

USF Tampa Graduate Theses and Dissertations

Thanks to the advancement of the modern computer simulation systems, many scientific applications generate, and require manipulation of large volumes of data. Scientific exploration substantially relies on effective and accurate data analysis. The shear size of the generated data, however, imposes big challenges in the process of analyzing the system. In this dissertation we propose novel techniques as well as using some known designs in a novel way in order to improve scientific data analysis.

We develop an efficient method to compute an analytical query called spatial distance histogram (SDH). Special heuristics are exploited to process SDH efficiently and accurately. …

The Use Of Textural Kinetic Habitats To Mine Diagnostic Information From Dce Mr Images Of Breast Tumors, Baishali Chaudhury

USF Tampa Graduate Theses and Dissertations

Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) of the breast is a widely used non-invasive approach to gather information about the underlying physiology of breast tumors. Recent studies indicate that breast tumor heterogeneity may reflect the presence of different levels of cellular aggressiveness or habitats within the tumor. This heterogeneity has been correlated to the variations in the contrast enhancement patterns within the tumor apparent on gadolinium-enhanced DCE-MRI. Although pathological and qualitative (based on contrast enhancement patterns) studies suggest the presence of clini- cal and molecular predictive tumor sub-regions, this has not been fully investigated in the quantitative domain.

The …

Mediation And Moderation Analysis Of Nutrition, Inflammatory Biomarkers, And Cognition In Older Adults, Elizabeth Handing

USF Tampa Graduate Theses and Dissertations

Nutrition can be viewed as a modifiable factor related to maintaining and preserving health in older adults. Previous studies have found that nutritional factors can influence cognitive abilities, however few studies have examined macronutrients and micronutrients as they relate to cognitive functioning. Research has yet to examine the mechanisms related to nutrition, cognition and aging in an older adult population from a holistic and interactive perspective.

This dissertation examined three research questions to better understand the relationship between age, nutrition, cognition, and inflammatory biomarkers. First, is nutrition related to cognition beyond demographic factors? Do individual nutrients serve as mediators? Second, …

Looking At Levels Of Medicalization In The Institutional Narrative Of Substance Use Disorders In The Military, Chase Landes Mccain

USF Tampa Graduate Theses and Dissertations

The purpose of this research is to examine the institutional narrative of substance use disorders (SUDs) in the U.S. military and the extent to which it reflects the medicalization process. Three general research questions guided my analysis of the narrative surrounding SUDs in the military: (1) How does the military characterize the problems and resolutions of SUDs? (2) How and to what extent does this narrative reflect medicalization? (3) What are the limitations inherent in the institutional narrative of SUDs in the military? In order to address these questions, I draw on three conceptual lenses: (1) The work of Loseke …

The Triumvirate Of Intersectionality: A Case Study On The Mobilization Of Domésticas In Brazil, Kristen Lei Nash

USF Tampa Graduate Theses and Dissertations

In this thesis, I look at the mobilization of the domestic workers in Brazil as a social movement. In Brazil, the domestic workers have managed to organize continuously for over eight decades using both informal and formal mechanisms to connect workers all over the country in unique ways. By viewing these women and the ways in which they have organized in the framework of a social movement, we can begin to identify their repertoires of contention and how those repertoires have contributed to the successes of the movement. In order to guide this investigation, I ask, how has the doméstica …

Effects Of Nicotine Withdrawal On Motivation, Reward Sensitivity And Reward-Learning, Jason A. Oliver

USF Tampa Graduate Theses and Dissertations

Research on addictive behavior has traditionally emphasized the role that primary reinforcing effects of drugs of abuse plays in the development and maintenance of dependence. However, contemporary behavioral economic theory and animal models of nicotine dependence suggest the need for greater attention to the impact that response to alternative rewards may have on smoking behavior. The present study sought to investigate the impact of nicotine withdrawal on self-report, behavioral and neural indices of motivation, immediate response to rewards and the capacity to learn and modify behavior in response to positive and negative feedback. Heavy smokers (n = 48) completed two …

Evidence Of Things Not Seen: A Semi-Automated Descriptive Phrase And Frame Analysis Of Texts About The Herbicide Agent Orange, Sarah Beth Hopton

USF Tampa Graduate Theses and Dissertations

From 1961 to 1971 the United States and the Republic of South Vietnam used chemicals to defoliate the coastal and upload forest areas of Viet Nam. The most notorious of these chemicals was named Agent Orange, a weaponized herbicide made up of two chemicals that, when combined, produced a toxic byproduct called TCDD-dioxin. Studied suggest that TCDD-dioxin causes significant human health problems in exposed American and Vietnamese veterans, and possibly their children (Agency, U.S. Environmental Protection, 2011). In the years since the end of the Vietnam War, volumes of discourse about Agent Orange has been generated, much of which is …

Rf Mems Resonators For Mass Sensing Applications, Ivan Fernando Rivera

USF Tampa Graduate Theses and Dissertations

Sensing devices developed upon resonant microelectromechanical and nanoelectromechanical (M/NEMS) system technology have become one of the most attractive areas of research over the past decade. These devices make exceptional sensing platforms because of their miniscule dimensions and resonant modes of operation, which are found to be extremely sensitive to added mass. Along their unique sensing attributes, they also offer foundry compatible microfabrication processes, low DC power consumption, and CMOS integration compatibility. In this work, electrostatically and piezoelectrically actuated RF MEMS bulk resonators have been investigated for mass sensing applications. The capacitively-transduced resonators employed electrostatic actuation to achieve desired resonance mode …

Impacts Of Agrochemical Pollution On Aquatic Communities And Human Disease, Neal T. Halstead

USF Tampa Graduate Theses and Dissertations

The global human population is expected to exceed 9 billion individuals by 2050, putting greater strain on the natural resources needed to sustain such a population. To feed this many people, some expect agricultural production will have to double and agrochemical use will have to increase anywhere from two- to five-fold relative to the turn of the century. Although industrial agriculture has provided many benefits to society, it has caused declines in biodiversity, both directly (e.g., through conversion of habitat) and indirectly (e.g., through contamination of adjacent natural habitats). Agricultural activity has also been linked to increased prevalence and intensity …

Obesity As A Disease: Effects On Weight-Biased Attitudes And Beliefs, Rheanna Nichole Ata

USF Tampa Graduate Theses and Dissertations

In June 2013, the American Medical Association (AMA) made the highly controversial decision to designate obesity a disease. Proponents predicted the decision would lead to reduced weight-related stigma, whereas opponents predicted designating a third of the population as “diseased” would exacerbate stigma. To determine the effects of defining obesity as a disease on explicit and implicit weight-biased attitudes and explicit weight-biased beliefs, female undergraduate students (N = 146) were randomly assigned to one of two groups: disease or lifestyle. Participants in the disease group (n = 71) were asked to read an article describing obesity as a disease caused by …

Sleep, Depressive Symptoms And Cognition In Older Adults And Caregivers Of Persons With Dementia, Glenna Shemida Brewster

USF Tampa Graduate Theses and Dissertations

Caregivers of persons with dementia, who are often older adults, report sleep disturbance, high rates of depressive symptoms and may be at risk for impaired cognition. This dissertation examined sleep, depressive symptoms, and cognition in older adults and caregivers of persons with dementia. The aims of the review of literature were to understand, in community dwelling adults 60 years and older, the relationships among sleep parameters (sleep onset latency, wake after sleep onset, sleep efficiency, total sleep time, and general sleep complaints), and the domains of cognition (Executive Function, Attention, Episodic Memory, Working Memory, Processing Speed), and global cognition. Based …

Exploring The Underlying Mechanisms Of Comorbid Adhd And Eating Disorders, Jennifer Rebecca Bleck

USF Tampa Graduate Theses and Dissertations

Evidence suggests comorbidity of ADHD and eating disorders (EDs) among females. Capitalizing on the comorbidity of ADHD and EDs and subsequent obesity could lead to improved prevention and treatment of all three conditions. However, additional information regarding the comorbidity is necessary to develop such interventions, as little is known about how or why this co-occurrence exists. A comprehensive model of the underlying mechanisms associated with comorbid ADHD and EDs is needed to improve understanding of the development of the comorbidity. Moreover, while there are gender differences within each disorder, literature is limited regarding to the comorbidity among males, leading to …

Straight Benevolence: Preserving Heterosexual Authority And White Privilege, Robb James Bruce

USF Tampa Graduate Theses and Dissertations

This thesis analyzes three current and popular media sites, exploring a term I coin “straight benevolence.” An ostensibly supportive and progressive attitude adopted by heterosexuals and expressed toward gay men in the United States, straight benevolence surreptitiously subordinates gayness and further entrenches white masculine heterosexual privilege. In my examination of hip-hop artist Macklemore’s “Same Love,” seven Major League Baseball “It Gets Better” gay-advocacy videos, and the “Suddenly, Last Summer” episode of ABC’s primetime U.S. television series Modern Family, I take an intersectional approach to address the interanimation of sexuality, gender, and race. I ask: In what ways is gay male …

Regulation And Targeting Of The Fancd2 Activation In Dna Repair, Valentina Celeste Caceres

USF Tampa Graduate Theses and Dissertations

Fanconi anemia (FA) is a genome instability syndrome that is clinically manifested by bone marrow failure, congenital defects, and elevated cancer susceptibility. The FA pathway is known to regulate the repair of DNA interstrand crosslinks in part through DNA homologous recombination (HR) repair. Up to today 16 FA proteins have been discovered that may participate in the common pathway. Cells that have mutations in the FA genes are hypersensitive to DNA damaging agents and display chromosome instability. A key regulatory event in the FA pathway is monoubiquitination of FANCD2-FANCI heterodimer that is mediated by a multi-component E3 ubiquitin ligase complex …

Functional Analysis Of The Ovarian Cancer Susceptibility Locus At 9p22.2 Reveals A Transcription Regulatory Network Mediated By Bnc2 In Ovarian Cells, Melissa Buckley

USF Tampa Graduate Theses and Dissertations

GWAS have identified several chromosomal loci associated with ovarian cancer risk. However, the mechanism underlying these associations remains elusive. We identify candidate functional Single Nucleotide Polymorphisms (SNPs) at the 9p22.2 ovarian cancer susceptibility locus, several of which map to transcriptional regulatory elements active in ovarian cells identified by FAIRE-seq (Formaldehyde assisted isolation of regulatory elements followed by sequencing) and ChIP-seq (Chromatin Immunoprecipitation followed by sequencing) in relevant cell types. Reporter and electrophoretic mobility shift assays (EMSA) determined the extent to which candidate SNPs had allele specific effects. Chromosome conformation capture (3C) reveals a physical association between Basonuclin 2 (BNC2) and …

The Relation Between Adiposity And Anxiety In Youth: Analysis Of Peer Victimization, Teasing, Sociocultural Influences, And Internalization Of Appearance Ideals As Explanatory Variables, Natasha L. Burke

USF Tampa Graduate Theses and Dissertations

The purpose of the current study was to examine putative mediators and moderators in the association between adiposity and anxiety in a sample of overweight and obese youth. In addition, anxiety was examined as a potential moderator between adiposity and health-related quality of life (HRQOL). Participants were youth (N = 137) between 8 and 17 years old (M = 13.09, SD = 2.61) and their legal caregivers recruited from four medical clinics affiliated with the University of South Florida. Youth were primarily overweight (28.5%) or obese (64.2%) and ethnically diverse. Data were analyzed by path analysis. Weight-related teasing significantly mediated …

Exploring The Pathogenic And Drug Resistance Mechanisms Of Staphylococcus Aureus, Whittney Burda

USF Tampa Graduate Theses and Dissertations

We have previously identified σS, an ECF sigma factor that is important in the virulence and stress response of S. aureus. Transcriptional profiling of sigS revealed that it is differentially regulated in a variety of laboratory and clinical strains of S. aureus, suggesting that there exists a regulatory network that modulates its expression. In order to identify direct regulators of sigS expression, we performed a biotin pull down assay in tandem with mass spectrometry. We identified CymR as a direct regulator and observed that sigS expression is increased in cells lacking cymR. In addition, transposon mutagenesis was performed to identify …

High-Throughput Screening Of Age-Related Changes In Caenorhabditis Elegans, Neil Copes

USF Tampa Graduate Theses and Dissertations

This project was developed to identify novel methods for high-throughput culturing and screening of C. elegans to investigate age-related metabolic changes and to survey the proteomic and metabolomic factors associated with age-related changes. To accomplish these goals we developed a novel way to grow C. elegans in liquid culture in 96-well microplates for several weeks without suffering significant fluid loss due to evaporation and without needing to shake or unseal the plates for aeration. We also developed methods for assaying the total volume of live C. elegans in microplate cultures using a fluorescence microplate reader and for performing RNAi experiments …

A Semiotic Phenomenology Of Homelessness And The Precarious Community: A Matter Of Boundary, Heather Renee Curry

USF Tampa Graduate Theses and Dissertations

My dissertation focuses on the articulation of the concepts of precarity —i.e., temporary, affective, creative, immaterial and insecure labor—and community in an overheating system. My site of inquiry is homelessness broadly, but more specifically the labor of panhandling and the identity of “the panhandler.” I recognize that primary theorizations of precarity have located it as a problem of labor and economy. Others have looked at it from the sociological domain. My work looks at precarity as diffuse across social, political, and communal systems, but primarily as an effect of the problem of overheating as it manifests at varying levels of …

Vulnerability Of Larval Fish Populations To Oil Well Blowouts In The Northern Gulf Of Mexico, Emily Chancellor

USF Tampa Graduate Theses and Dissertations

On April 20th, 2010, a fire broke out on the Deepwater Horizon (DWH) offshore oil drilling rig on the Macondo prospect located off the coast of the Gulf of Mexico (GOM). This fire and resulting explosions resulted in the sinking of the Deepwater Horizon rig and the largest marine oil spill in history. An estimated 4.9 million (+/- 10%) barrels were released into the Gulf of Mexico over the ensuing 87 days. Many economically important fish species spawn in northern Gulf of Mexico waters; the spawning seasons and pelagic larval phase of many of these species occur within the temporal …

Strategies For Preventing Age And Neurodegenerative Disease-Associated Mitochondrial Dysfunction, Vedad Delic

USF Tampa Graduate Theses and Dissertations

Mitochondrial dysfunction plays a pivotal role in the development of aging phenotypes and aging-associated neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and Amyotrophic lateral sclerosis (ALS). Strategies that restore mitochondrial dysfunction may rescue the deficits of central metabolism in these disorders and improve cell survival. For example, we found that modulating the mTOR signaling pathway in a tissue culture model of aging-induced mitochondrial DNA mutation enhanced mitochondrial function as evidenced by increased oxygen consumption. Our previous melatonin studies also led us to hypothesize that caloric restriction and the hormone melatonin would reverse brain mitochondrial dysfunction in animal …

The “Other” Side Of Wall Street: Banking, Policies, And Adaptive Methods Of U.S. Migrant Workers, Cassandra Rae Decker

USF Tampa Graduate Theses and Dissertations

Migrant farmworkers' social and economic mobility is frequently constrained through the denial of basic resources, such as access to the formal financial sector. This thesis ethnographically examines banking policies as they apply to low-income, mobile, populations that temporarily reside in Florida. It utilizes participant observation, interviews, and participatory mapping with migrant farmworkers. It also considers how policymakers and service providers in the formal and informal financial sectors rationalize control of resources and the effects on mobile populations. Particular attention is paid to adaptive practices in the alternative financial sector – cash checking services, carrying cash, and remittances. By utilizing the …

Compassion Fatigue And Self-Care Strategies Among Addiction Professionals: A Qualitative Study, Amanda Depippo

USF Tampa Graduate Theses and Dissertations

Addiction is a prevalent disorder and is on the rise. Addiction has serious symptomology and can negatively impact an individual’s life in several areas. Not only can the person addicted become negatively impacted, but the addiction professional that treats this disorder can become negatively impacted as well. Because there is often a high co-occurrence of addiction and trauma among addiction clients, this intensifies the clinical profile of such clients which compounds the risk for addiction professionals. It is the addiction professional’s response to this combination of addiction and trauma that can cause these professionals to experience compassion fatigue.

Research has …

The Continuum Of Ethno-Racial Socialization: Learning About Culture And Race In Middle-Class Latina/O Families, Maria D. Duenas

USF Tampa Graduate Theses and Dissertations

This thesis examines the discursive messages and specific practices that Latino families use to transmit messages about culture, race, and racism. Scholars have not fully explored the complexity and range of practices and discourses that are involved in Latinos’ ethno-racial socialization. The use of the phrase “ethno-racial socialization” is important because it combines the concepts of racial socialization and ethnic socialization in an effort to account for how the lived experiences of Latinos who mostly think of themselves as a racial group, are treated as one race, and thus, discuss race with family members. This research explores this process using …

Genomic Aberrations At The 3q And 14q Loci: Investigation Of Key Players In Ovarian And Renal Cancer Biology, Punashi Dutta

USF Tampa Graduate Theses and Dissertations

Genomic aberrations are primary contributors to the pathophysiology of cancer [11]. Dysregulated expression of genes located within these aberrations are important predictors of chemoresistance, disease prognosis, and patient outcome [12]. This dissertation is focused on understanding the regulation and/or functions of specific genes located at dysregulated genomic regions such as 3q26 and 14q32 in the biology of ovarian and renal cancer, respectively.

Serous epithelial ovarian cancer (EOC) manifest amplification at the 3q26.2 locus [2], an observation consistent with the cancer genome atlas (TCGA) [13]. The most amplified gene in this region is EVI1 which has been extensively studied in hematological …

The Effects Of Supplemented Metabolites On Lifespan And Stress Response Pathways In Caenorhabditis Elegans, Clare B. Edwards

USF Tampa Graduate Theses and Dissertations

Understanding how metabolites contribute to anaplerosis, antioxidant effects, and hormetic pathways during aging is fundamental to creating supplements and dietary habits that may decrease age-associated disease and decline, thus improving the quality of life in old age. In order to uncover metabolic pathways that delay aging, the effects of large sets of metabolites associated with mitochondrial function on lifespan were investigated.

Malate, the tricarboxylic acid (TCA) cycle metabolite, increased lifespan and thermotolerance in C. elegans. Addition of fumarate and succinate also extended lifespan and all three metabolites activated nuclear translocation of the cytoprotective DAF-16/FOXO transcription factor and protected from paraquat-induced …

Maternal Stress, Breastmilk Igf-1, And Offspring Growth Among Breastfeeding Mothers-Infant Pairs In The Tampa Bay Area, Lauren Michelle Gottfredson

USF Tampa Graduate Theses and Dissertations

Background: Maternal stress during utero has been shown to have negative health consequences on the offspring, including low birth weight and increased risk of adult disease. Variation in breastmilk may act as an environmental cue of maternal stress and continue to program the infant during early life. This research aimed to explore the role of breastmilk on developmental programming of the infant. Specifically, to examine how breastmilk may act as a medium for the exposure of stress between the mother and the offspring, and see if variation in insulin like growth factor-1 (IGF-1) a potential mechanism for the relationship.

Methods: …

Mmv Malaria Box Activity Screening In Dormant Plasmodium Falciparum Phenotypes, Sandra Galusic

USF Tampa Graduate Theses and Dissertations

The causative agent of malignant tertian malaria, Plasmodium falciparum undergoes an arrested growth phenotype of its erythrocytic stage when under drug-stress. Recent artemisinin treatment failures seem to be indicative of such induction followed by recrudescence rather than actual therapeutic failure. Likewise, P. vivax hypnozoites are the prototypic dormants and the latent infections for which they are responsible prove most difficult to treat. Dihydroartemisinin, an artemisinin-derivative, can be used to exploit this mechanism by inducing a dormant state in ring-stage P. falciparum parasites and in turn, their recovery may be used as a screening period for compounds that inhibit or foster …