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Ironic Masculinity And Femininity: Do Contextual Factors Reverse Attributions Based On Gender Stereotyped Behaviors?, Kenneth S. Michniewicz

USF Tampa Graduate Theses and Dissertations

Emerging research highlights the social penalties for men and women who commit cross-gendered behaviors. Here, I examine how and when two contextual mechanisms (competence and credentials) alter people's perceptions of cross-gendered behavior and render actors as less gender-atypical and more gender-typical. In Study 1, I tested the hypothesis that incompetence in cross-gendered behaviors would communicate same-gendered qualities by contrast. In Study 2, I tested the hypothesis that an actor who commits a cross-gendered behavior will receive less gender-inconsistent evaluations if they first demonstrate gender-typical traits. Moreover, Study 2 examines whether or not these credentialed actors change the perception of the …

Dcms: A Data Analytics And Management System For Molecular Simulation, Meryem Berrada

USF Tampa Graduate Theses and Dissertations

Despite the fact that Molecular Simulation systems represent a major research tool in multiple scientific and engineering fields, there is still a lack of systems for effective data management and fast data retrieval and processing. This is mainly due to the nature of MS which generate a very large amount of data - a system usually encompass millions of data information, and one query usually runs for tens of thousands of time frames. For this purpose, we designed and developed a new application, DCMS (A data Analytics and Management System for molecular Simulation), that intends to speed up the process …

The Detection And Quantitative Analysis Of Endocannabinoids And Endogenous Fatty Acid Amides In Apis Mellifera And Tribolium Castaneum, Perry Robert Mitchell Jr.

USF Tampa Graduate Theses and Dissertations

Endocannabinoids, and the fatty acid amides from which they are a member, have garnered greater scientific interest in the last two decades due to the cannabimimetic properties of these endogenous molecules. The most well-known of these is Anandamide, which has thus far been discovered in several species of animal ranging from C. elegans, fruit flies, to bovine and humans. Because of the importance and increasing impact of these compounds a brief overview is first presented herein, with a major focus on the N-acyldopamines due to the direct impact they potentially pose to human physiology.

Secondly, the detection and …

Solids Accumulation Rates Of Latrines At Rural Schools In Nimba County, Liberia, Caraline M. Murphy

USF Tampa Graduate Theses and Dissertations

Access to appropriate sanitation facilities as well as access to clean drinking water are considered fundamental human rights (Carr, 2001; Bjorklund & Sjodin, 2010), yet roughly 2.5 billion people on the planet lack access to an improved form of sanitation (WHO/UNICEF, 2014). Additionally, many entities responsible for emergency excreta management and sanitation management design guidelines, specifically solids accumulation rates in latrine pits, use rates that are 30-60 years old and may be established from dated knowledge on diet and lifestyle trends (Franceys et al., 1992; Harvey et al., 2002; Harvey, 2007). Using solids accumulation rates that are dated as well …

The Archaeology Of The Mckinnie Site (8ja1869), Apalachicola River Valley, Northwest Florida: Four Thousand Years In The Backswamp, Eric D. Prendergast

USF Tampa Graduate Theses and Dissertations

This research describes a large, newly-recorded archaeological site in the Upper Apalachicola River valley, northwest Florida, and a private collection of artifacts from it, as well as test excavations, three-dimensional modeling, clay/pottery sourcing through chemical analysis, and direct radiocarbon dating of ceramics to relate the site with regional archaeological chronologies and settlement patterns. A University of South Florida (USF) 2013 field school conducted excavations at the multicomponent midden on the western floodplain of the Apalachicola River called the McKinnie site (8JA1869). Students collaborated with a local collector and family members to learn about the site's history. Data from the collection …

Women's Narratives Of Confinement: Domestic Chores As Threads Of Resistance And Healing, Jacqueline Marie Smith

USF Tampa Graduate Theses and Dissertations

The term "narratives of confinement" redefines the parameters by which first-person, fictive and non-fictive, accounts of female captivity are classified, broadening the genre beyond Indian captivity narratives and slave narratives to include other works in which female narrators describe physical and/or psychological confinement due to tangible or non-tangible forces. Often these narratives exhibit the transformation of the drudgery of housewifery into powerful symbols of resistance and subversion, especially in reaction to traumatic events related to confinement. Needlework and food, including its preparation and distribution, frequently emerge as metaphors that express the ways in which disempowered women seek to regain control …

The Encultured Mind: From Cognitive Science To Social Epistemology, David Alexander Eck

USF Tampa Graduate Theses and Dissertations

There have been monumental advances in the study of the social dimensions of knowledge in the late twentieth and early twenty-first centuries. But it has been common within a wide variety of fields--including social philosophy, cognitive science, epistemology, and the philosophy of science--to approach the social dimensions of knowledge as simply another resource to be utilized or controlled. I call this view, in which other people's epistemic significance are only of instrumental value, manipulationism. I identify manipulationism, trace its manifestations in the aforementioned fields, and explain how to move beyond it. The principal strategy that I employ for moving beyond …

Does Emotional Processing Mediate The Link Between Disordered Sleep And Depression?, Kimberly O'Leary

USF Tampa Graduate Theses and Dissertations

Disordered sleep is strongly linked to depression, but reasons for this are not well understood. One possibility is that this link is partially explained by deficits in the emotional processing system. This model is substantiated based on the strong link between sleep and emotions, as well as ties between affect and depression. Therefore, this study tested whether various emotional and non-emotional deficits mediated the link between poor sleep quality and depression. Two hundred undergraduate students were recruited via an online university system. Participants completed self-report scales of depression, sleep quality, emotion recognition, and affective response to pre-tested pleasant or unpleasant …

Investigation Of Opuntia Ficus-Indica Mucilage Nanofiber Membrane Filtration For Water Systems, Rasudha Muppaneni

USF Tampa Graduate Theses and Dissertations

This work investigates the fabrication, characterization and testing of Opuntia ficus-indica mucilage nanofibers to be utilized in water filtration systems. These mucilage nanofibers are formed using different polymers through a process called electrospinning. The polymers used to promote the formation of nanofibers are poly vinyl alcohol (PVA) and polystyrene (PS). The mucilage is a jelly like substance extracted from the pads of the cactus plant. It is a mixture of proteins, complex polysaccharides and monosaccharaides. It is an inexpensive, non-toxic, biodegradable and biocompatible material which is present in abundance. The mucilage extracted from the pads is mixed with acetic acid …

Navigating Collective Activity Systems: An Approach Towards Rhetorical Inquiry, Katherine Jesse Royce

USF Tampa Graduate Theses and Dissertations

The purpose of this formative intervention was to design a professional and technical communications course around rhetorical inquiry. The participants, undergraduate health sciences majors (N=22 for section A, N=20 section B), were observed throughout the fall semester of the 2014-2015 academic year. A rhetorical inquiry framework was applied via activity systems, and data were collected using several methodologies including participant observations, research questionnaires, as well as participant deliverables, and were transcribed using Daisy Mwanza's Eight-Step Model. Results demonstrated students successfully used activity systems as a means of approaching rhetorical inquiry. Furthermore, students indicated a high level of engagement in the …

Parent-Child Interaction Therapy As A Treatment For Adhd In Early Childhood: A Multiple Baseline Single-Case Design, Kendall Jeffries Deloatche

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine the effectiveness of PCIT as an alternative to medication in managing symptoms and behavior problems of preschool-aged children with ADHD. Using a multiple baseline single-case design, the study measured the impact of PCIT on four preschool-aged children's problem behaviors and ADHD symptoms, parenting practices, and mothers' attitudes towards therapy. Outcome measures included the Child Behavior Checklist, Eyberg Child Behavior Inventory, Behavior Assessment System for Children, ADHD Symptom Observation form, Dyadic Parent-Child Interaction Coding System, Parenting Practices Interview, and Therapy Attitude Inventory. Results from visual analyses, a visual permutation test, and hierarchical linear …

Synthesis, Characterization, And Electrochemical Properties Of Polyaniline Thin Films, Soukaina Rami

USF Tampa Graduate Theses and Dissertations

Conjugated polymers have been used in various applications (battery, supercapacitor, electromagnetic shielding, chemical sensor, biosensor, nanocomposite, light-emitting-diode, electrochromic display etc.) due to their excellent conductivity, electrochemical and optical properties, and low cost. Polyaniline has attracted the researchers from all disciplines of science, engineering, and industry due to its redox properties, environmental stability, conductivity, and optical properties. Moreover, it is a polymer with fast electroactive switching and reversible properties displayed at low potential, which is an important feature in many applications. The thin oriented polyaniline films have been fabricated using self-assembly, Langmuir-Blodgett, in-situ self-assembly, layer-by-layer, and electrochemical technique. The focus of …

Let's Go To The Carnival: Hybridization Of Heterotopian Spaces In The Films Of Kevin Smith, Anthony L. Sylvester

USF Tampa Graduate Theses and Dissertations

This paper argues against the charges of puerility in the films of Kevin Smith. I analyze Mallrats (1995), Clerks II (2006) and Zack and Miri Make a Porno (2008). To illustrate my contention, I offer close readings of the director's films, particularly the protagonists' bodily/linguistic performances. My efforts will vindicate my assertion that through these specialized performances, through the forceful assertion of their marginal identities, the films' protagonists encroach upon, and finally appropriate, historically dominant spaces. As a result, the spaces they appropriate acquire a new, characteristic hybridity. Michel Foucault's concept of heterotopia provides a framework for delineating the dominant …

Promoting Genome Stability Via Multiple Dna Repair Pathways, Scott Cukras

USF Tampa Graduate Theses and Dissertations

Maintaining genome integrity is indispensible for cells to prevent and limit accruement of deleterious mutations and to promote viable cell growth and proliferation. Cells possess a myriad of mechanisms to detect, prevent and repair incurred cellular damage. Here we discuss various proteins and their accompanying cellular pathways that promote genome stability. We first investigate the NEDD8 protein and its role in promoting homologous recombination repair via multiple Cullin E3 ubiquitin ligases. We provide specific mechanisms through which, UBE2M, an E2 conjugating enzyme, neddylates various Cullin ligases to render them catalytically active to degrade their substrates by the proteasome. We show …

Reproductive Health Seeking Behaviors Among Female University Students: An Action Oriented Exploratory Study, Robin Emily Mowson

USF Tampa Graduate Theses and Dissertations

The focus of this research was to: 1) study the perceptions of female students attending the university Student Health Center, concerning available services, 2) learn how they describe their decisions to obtain care, and 3) identify perceived barriers to reproductive health care and contraception. This exploratory study used a mixed-methods approach that included clinic public-space observations, interviews with health care providers and staff at Student Health Services (SHS), surveys distributed to clients of the campus clinic, and in-depth interviews to contextualize emergent themes. Topics addressed included sexual health behaviors and perceptions, influence of peers and partners, the propagation of health …

5-Aminolevulinate Synthase: Characterization Of The Enzymatic Mechanism, Reaction Selectivity, And Structural Plasticity, Bosko M. Stojanovski

USF Tampa Graduate Theses and Dissertations

5-Aminolevulinate synthase (ALAS) catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation between glycine and succinyl-CoA to generate coenzyme A (CoA), CO₂, and 5-aminolevulinate (ALA). The chemical mechanism of this reaction, which represents the first and regulated step of heme biosynthesis in mammals, involves the formation of a short-lived glycine quinonoid intermediate and an unstable 2-amino-3-ketoadipate intermediate. Using liquid chromatography coupled with tandem mass spectrometry to analyze the products from the reaction of murine erythroid ALAS (mALAS2) with O-methylglycine and succinyl-CoA, we directly identified the chemical nature of the inherently unstable 2-amino-3-ketoadipate intermediate, which predicates the glycine quinonoid species as …

Stress And Coping In High School Students In Accelerated Academic Curricula: Developmental Trends And Relationships With Student Success, Brittany V. Hearon

USF Tampa Graduate Theses and Dissertations

High school students in accelerated academic curricula including Advanced Placement (AP) courses and International Baccalaureate (IB) programs are faced with unique challenges associated with their rigorous academic demands, in addition to normative adolescent stressors. Because of the increasing popularity of AP and IB among high-achieving youth and benefits realized by students who successfully manage such curricula, there remains a need to better understand the experiences of stress and coping among this population. The current study used longitudinal and cross-sectional comparisons to (a) investigate the degree to which students in accelerated curricula experience environmental stressors and employ coping strategies to manage …

The Association Of Cognitive Endophenotypes And Risky Single Nucleotide Polymorphisms Of Alzheimer's Disease Within The Alzheimer's Disease Neuroimaging Initiative (Adni) Database, Kyle Joseph Jennette

USF Tampa Graduate Theses and Dissertations

Objective: The purpose of this study was to assess the influence of three single nucleotide polymorphisms (SNP) previously associated with Alzheimer's disease on specific domains of cognition, when controlling for Apolipoprotein E gene (APOE), in a sample of individuals with Alzheimer's disease. Methods: The data were drawn from the Alzheimer's Disease Neuroimaging Initiative database, a comprehensive, longitudinal database of controls, persons with mild cognitive impairment, and persons with mild Alzheimer's disease. Each subject has a full neuropsychological assessment, neuroimaging, genetic sequencing, and physical evaluation. For the purposes of this study, individuals were selected based on the presence of the three …

Design, Synthesis And Applications Of Polymer Biomaterials, Frankie Costanza

USF Tampa Graduate Theses and Dissertations

The emergence of antibiotic resistant bacteria has prompted the research into novel kinds of antibacterial small molecules and polymers. Nature has solved this issue with the use of cationic antimicrobial peptides, which act as nonspecific antibiotics against invading species. Herein, we have tried to mimic this general mechanism in a biocompatible and biodegradable polymer micelle based on the polymerization of naturally occurring amino acids lysine and phenylalanine linked to a PEG tether. This amphiphilic structure allows for the spontaneous collapse into stable nanoparticles in solution, which contains a hydrophilic outer layer and a hydrophobic core. Our polymers have shown activity …

A Functional Chlorophyll Biosynthesis Pathway Identified In The Kleptoplastic Sea Slug, Elysia Chlorotica, Julie A. Schwartz

USF Tampa Graduate Theses and Dissertations

The sacoglossan sea slug, Elysia chlorotica, feeds upon and sequesters plastids from the heterokont alga, Vaucheria litorea, and maintains the metabolically active organelles for up to nine months under starvation conditions while utilizing the photosynthate to survive and reproduce. The photosynthetic pigment, chlorophyll a (Chla), is found in all oxygenic photosynthetic organisms and is responsible for capturing photons of light and converting them into chemical energy. Chlorophyll and its associated proteins involved in the light capturing process are subject to photo oxidative damage and must be continually replaced for ongoing photosynthesis to continue; however, genes encoding these proteins are present …

Utilizing Systems Level Approaches To Identify Key Mechanisms Of Drug Resistance In Braf Mutated Melanoma, Kim H.T. Paraiso

USF Tampa Graduate Theses and Dissertations

In the last four years, seven new drugs have been FDA approved for the treatment of late stage melanoma, for the field of melanoma, this marks an incredibly exciting. Three of these new therapies, vemurafenib, dabrafenib and trametinib are small molecule kinase inhibitors that target the MAPK pathway and as such have been approved for the treatment of BRAFV600 mutant melanomas. Yet despite recent advances, mechanisms of intrinsic and acquired BRAF inhibitor resistance continue to undermine uniform and long-lasting therapeutic responses. Several studies have shown that the reactivation of MAPK signaling is a critical event leading to BRAF inhibitor resistance. …

Gamma-Aapeptides As A New Class Of Peptidomimetics: Synthesis, Structures, And Functions, Haifan Wu

USF Tampa Graduate Theses and Dissertations

Peptidomimetics are synthetic oligomers that resemble the activities of peptides. Their advantages over peptides include high stability towards proteolysis and enormous chemical diversity. Over the past two decades, there have been extensive efforts to develop peptide mimics, such as beta-peptides, peptoids, D-peptides, etc. The research on peptidomimetics have led to many important applications in both medicinal and material science. In order to explore new functions, the discovery of peptidomimetics with novel frameworks is essential. We reported the synthesis and evaluation of a new class of peptidomimetics, termed as gamma-AApeptides. Previous studies of gamma-AApeptides have revealed that gamma-AApeptides are highly resistant …

Design And Synthesis Of Bioactive Peptidomimetics, Yaogang Hu

USF Tampa Graduate Theses and Dissertations

Protein-Protein Interactions (PPIs) play a very important role in biological functions and therefore the inhibition of specific Protein-Protein Interactions has a huge therapeutic value. The most successful small molecular PPIs inhibitors do not fit with the prevalent `Rule of Five' drug profile. To overcome the disadvantages of small molecular PPIs inhibitors, peptide based PPIs inhibitors were developed. Herein we describe the development of a new class of peptidomimetics AA-peptides. The AApeptides were designed based on chiral PNA backbone. Substitution of nucleobases yields AApeptides that are resistant to proteolysis and capable of mimicking peptides. Two types of AApeptides were discussed in …

Optimization Of Aggregating Agents And Sers Substrates For Sers Detection Of Cotinine And Trans 3'-Hydroxycotinine, Sungyub Han

USF Tampa Graduate Theses and Dissertations

This dissertation mainly focuses on applications of Surface Enhance Raman Scattering (SERS) to detect tobacco-related biomarkers with optimized experimental conditions (pH and aggregating agents) and SERS substrates (silica core and silver shell nanoparticles). Cotinine (COT) and trans 3-hydroxycotinine (3HC), metabolized from nicotine as one of main chemicals of tobacco, have been used as tobacco biomarkers because their half-life are longer than that of nicotine, which enable to monitor the tobacco exposure. The effects of aggregating agents and pH on SERS detection of COT and 3HC were investigated. Aggregating agents play an important role in SERS detection of target molecules since …

Coherent Digital Holographic Adaptive Optics, Changgeng Liu

USF Tampa Graduate Theses and Dissertations

A new type of adaptive optics (AO) based on the principles of digital holography (DH) is proposed and developed for the use in wide-field and confocal retinal imaging. Digital holographic adaptive optics (DHAO) dispenses with the wavefront sensor and wavefront corrector of the conventional AO system. DH is an emergent imaging technology that gives direct numerical access to the phase of the optical field, thus allowing precise control and manipulation of the optical field. Incorporation of DH in an ophthalmic imaging system can lead to versatile imaging capabilities at substantially reduced complexity and cost of the instrument. A typical conventional …

Status Of A Translocated Florida Sand Skink Population After Six Years: Establishing And Evaluating Criteria For Success, Adam Ryan Emerick

USF Tampa Graduate Theses and Dissertations

The translocation of organisms is becoming a frequently used tool in conservation biology. There are, however, a disproportionate number of unsuccessful attempts translocating populations of herpetofauna. Logistical and temporal limits of monitoring, combined with ambiguous metrics concerning "success," have led to few advances regarding reptile translocations. Successfully established and persistent populations are those in which both the founding population and subsequent generations show consistent or positive levels of survival and reproduction. A small population of the threatened Florida Sand Skink (Plestiodon reynoldsi) was translocated in 2007. Data collected from 2007 to 2009 confirmed survival and reproduction among the founding individuals, …

Identification And Characterization Of N-Acyltransferase Enzymes That Are Involved In The Biosynthesis Of Fatty Acid Amides, Daniel Robert Dempsey

USF Tampa Graduate Theses and Dissertations

Fatty acid amides are an emerging family of bioactive lipids that consists of N-acylethanolamines, N-acylarylalkylamides, N-acylglycines, N-acyl amino acids, N-monoacylpolyamides, and primary fatty acid amides. Short chain fatty acid amides are products of inactivated biogenic amines such as dopamine, histamine, octopamine, and serotonin, whereas long chain fatty acid amides have been implicated in a number of physiological process such as the perception and inhibition of chronic pain through binding to their specific receptors. The most famous; therefore, the most studied long chain fatty acid amide is anandamide or also known as N-arachidonylethanolamine. The biosynthesis …

The Effects Of Repetitive Thought And Construal Level On Alcohol Consumption, Andrew Mark Kiselica

USF Tampa Graduate Theses and Dissertations

Repetitive thought, or the recurrent, often cyclical, focus on self-relevant concerns and experiences, is one liability that may be common across internalizing (INT) and externalizing (EXT) disorders. One particular area of interest for examining repetitive thought as a transdiagnostic process is in relation to alcohol use because alcohol abuse and dependence are the most common, and possibly most costly, EXT disorders. This study experimentally induced abstract repetitive thought, concrete repetitive thought, or distraction to test if repetitive thought and construal level have an effect on drinking behavior. It was hypothesized that individuals in both repetitive thought conditions would drink more …

An Evaluation Of An Electronic Student Response System In Improving Class-Wide Behavior, Ashley Horne

USF Tampa Graduate Theses and Dissertations

A student response system is a technology that allows an entire classroom of students to respond to questions and receive immediate feedback from teachers during instruction. However, little research has examined the use of student response systems to support student behavior in elementary schools. This study focused on using an electronic student response system to improve class-wide behavior in two general elementary school classrooms. An ABAB and ABA reversal designs embedded within a multiple baseline design across classrooms was employed to evaluate the outcome of the intervention. Although limited, the results indicated that the classroom teachers implemented the electronic student …

Reducing The Control Burden Of Legged Robotic Locomotion Through Biomimetic Consonance In Mechanical Design And Control, Caitrin Elizabeth Eaton

USF Tampa Graduate Theses and Dissertations

Terrestrial robots must be capable of negotiating rough terrain if they are to become autonomous outside of the lab. Although the control mechanism offered by wheels is attractive in its simplicity, any wheeled system is confined to relatively flat terrain. Wheels will also only ever be useful for rolling, while limbs observed in nature are highly multimodal. The robust locomotive utility of legs is evidenced by the many animals that walk, run, jump, swim, and climb in a world full of challenging terrain.

On the other hand, legs with multiple degrees of freedom (DoF) require much more complex control and …