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The Fate Of Fecal Indicator Bacteria In Passive Nitrogen Reduction Systems, Michelle B. Henderson

USF Tampa Graduate Theses and Dissertations

The sustainability of water resources is an international and national concern. With increased human activity, water distribution on a global, regional, and local scale has been negatively impacted. Managing water resources also includes managing wastewater. A promising solution to the issues of water scarcity and distribution in the environment is the reuse of wastewater. Wastewater is produced from various sources (domestic, industrial, and commercial); however, if this water is able to be reused closer to the source of generation it could positively impact water distribution. In the United States, approximately 25% of domestic wastewater is treated in onsite wastewater treatment …

Freshwater Wetland Creation In A Changing Urban Environment: Designing For Long-Term Viability, Aaron Thomas Ryan Brown

USF Tampa Graduate Theses and Dissertations

Urbanization and wetland mitigation are increasingly common in coastal watersheds with expanding populations. These mitigation wetlands are intended to offset the functional and structural losses experienced when natural systems are degraded or destroyed. In the Tampa Bay watershed, urbanization is both expanding into the upper reaches of the watershed and intensifying in previously-developed areas, resulting in the creations of hundreds of freshwater mitigation wetlands. This dissertation utilized an existing database of mitigation wetlands, publicly available data, and field surveys to investigate the relationship between constructed wetlands and their surroundings and also determine how design affects wetland condition over time. The …

Subtropical Benthos Vary With Reef Type, Depth, And Grazing Intensity, Kara R. Wall

USF Tampa Graduate Theses and Dissertations

Marine epibenthic communities are influenced by both pre- and post-recruitment processes. For instance, the larval supply and cues that influence settlement (pre-recruitment), as well as the growth and mortality of individuals (post-recruitment), may differ across reef type and depth. Determining the relative influence of these processes is important to understanding how epibenthic communities can develop in a region. Using both a recruitment experiment that controlled grazing by urchins and in situ photographic surveys of epibenthic communities, this study examined the recruitment and composition of epibenthos on natural limestone and artificial reefs in the eastern Gulf of Mexico (eGOM). In the …

Modeling Of Dynamic Allostery In Proteins Enabled By Machine Learning, Mohsen Botlani-Esfahani

USF Tampa Graduate Theses and Dissertations

Regulation of protein activity is essential for normal cell functionality. Many proteins are regulated allosterically, that is, with spatial gaps between stimulation and active sites. Biological stimuli that regulate proteins allosterically include, for example, ions and small molecules, post-translational modifications, and intensive state-variables like temperature and pH. These effectors can not only switch activities on-and-off, but also fine-tune activities. Understanding the underpinnings of allostery, that is, how signals are propagated between distant sites, and how transmitted signals manifest themselves into regulation of protein activity, has been one of the central foci of biology for over 50 years. Today, the importance …

The Effects Of Maternal Folate On Fetal Brain And Body Size Among Smoking Mothers, Korede K. Adegoke

USF Tampa Graduate Theses and Dissertations

The adverse effects of maternal smoking on infant mortality and morbidity has been well documented in the literature. Maternal tobacco use is causally associated with fetal growth restriction and correlates negatively with folate intake and metabolism. Studies have examined the association between smoking and folate levels during pregnancy, but very few have assessed this relationship using objective and accurate measures of both variables. Furthermore, despite evidence of a causal association between smoking in pregnancy and intrauterine growth restriction, and a plausible relationship between tobacco use and low maternal folate which is required for optimal fetal growth, no experimental study has …

Osteopontin Plays A Pivotal Role In In Increasing Severity Of Respiratory Syncytial Virus Infection, Viviana Sampayo-Escobar

USF Tampa Graduate Theses and Dissertations

The molecular mechanisms underlying susceptibility to severe respiratory syncytial virus (RSV) infection remain poorly understood. Herein, we report on the role of osteopontin (OPN) in regulation of RSV infection in human epithelial cells and how interleukin-1 beta (IL-1β), a cytokine secreted soon after RSV infection, when persistently expressed can induce OPN expression leading to increased viral infection. We first compared OPN expression in two human epithelial cell lines: HEK-293 and HEp-2. In contrast to HEp-2, HEK-293 expresses low levels of pro-caspase-1 resulting in decreased IL-1β expression in response to RSV infection. We found a correlation between low IL-1β levels and …

Magnetic Nanostructure Application In Sers Detection Of Melatonin And Resonance Raman Characterization Of Binding Of Catechol Moieties With Antibiotic, Siqi Sun

USF Tampa Graduate Theses and Dissertations

Raman spectroscopy provides characteristic peaks of the target molecules that can be observed with appropriate laser, spectrometer and detector. These characteristic peaks can reveal ‘fingerprint’ information of a molecule even in a myriad of cases and combinations. It makes Raman spectroscopy a highly sensitive and selective analytical technique. Raman spectroscopy has a variety of chemical, environmental and biological applications. In this dissertation, modified Raman method are applied in life sciences and pharmaceutical studies.

The major challenge against development of Raman spectroscopy is how to improve the intensity of Raman signal. Surface Enhance Raman Scattering (SERS) and resonance Raman(RR) are utilized …

Regulation Of Extracellular Signal-Regulated Kinase By Histone Deacetylase 6, Jheng-Yu Wu

USF Tampa Graduate Theses and Dissertations

Extracellular signal-regulated kinases 1/2 (ERK1/2) are important kinases regulating cell proliferation and cell migration, and have been established as therapeutic targets for cancer treatment. Previously, we found that ERK1 phosphorylates histone deacetylase 6 (HDAC6) to regulate its enzymatic activity. However, whether HDAC6 reciprocally modulates ERK1 activity is unknown. Here, we have discovered that ERK1/2 are acetylated proteins and shown that HDAC6 manipulates ERK1’s kinase activity via deacetylation. We demonstrated that both ERK1 and ERK2 interact with HDAC6 physically. We showed that the acetylation level of GST-ERK1/2 increased in a dose- and time-dependent manner upon treatment with a pan-HDAC inhibitor, Trichostatin …

Ambient Benzene And Pm2.5 Exposure During Pregnancy: Examining The Impact Of Exposure Assessment Decisions On Associations Between Birth Defects And Air Pollution, Jean Paul Tanner

USF Tampa Graduate Theses and Dissertations

In the United States, approximately 3% of infants are diagnosed with a major birth defect each year. Whereas prevention efforts have led to decreases in some birth defects, the cause of approximately 70% of birth defects remains unknown. More recently, there has been increasing concern regarding exposures to environmental agents, such as air pollution during pregnancy, and the risk of birth defects. Over the past decade, there has been an increase in research studies examining the association between air pollution and birth defects. The results have been inconsistent, with some studies reporting that higher levels of exposure from the same …

Viruses In Marine Animals: Discovery, Detection, And Characterizarion, Elizabeth Fahsbender

USF Tampa Graduate Theses and Dissertations

Diseases in marine animals are emerging at an increasing rate. Disease forecasting enabled by virus surveillance presents a proactive solution for managing emerging diseases. Broad viral surveys aid in disease forecasting by providing baseline data on viral diversity associated with various hosts, including many that are not associated with disease. However, these viruses can become pathogens due to expansion in host or geographic range, as well as when changing conditions shift the balance between commensal viruses and the host immune system. Therefore, it is extremely valuable to identify and characterize viruses present in many different hosts in a variety of …

Essays On Crowdfunding: Exploring The Funding And Post-Funding Phases And Outcomes, Onochie Fan-Osuala

USF Tampa Graduate Theses and Dissertations

In the recent years, crowdfunding (a phenomenon where individuals collectively contribute money to back different goals and projects through the internet) has been gaining a lot of attention especially for its socio-economic impact. This dissertation explores this phenomenon in three distinct but related essays. The first essay explores the nature and dynamics of backers’ contributions and uses the insights generated to develop a forecasting model that can predict crowdfunding campaign outcomes. The second essay investigates how creators’ crowdfunding campaign design decisions impact their funding and post-funding outcomes. Interestingly, the essay highlights that certain crowdfunding campaign design decisions have differential effects …

Distribution Of Polybrominated Diphenyl Ethers Among Demographic Categories, Giorvanni Merilis

USF Tampa Graduate Theses and Dissertations

Polybrominated Diphenyl Ethers (PBDEs) are flame retardants widely used within the United States in various products such as plastics, electronics, textiles and furniture. With an increase in production and usage, PBDEs have recently emerged as a contaminant of concern. Due to their chemical structure, PBDEs have the propensity to bioaccumulate in mammals. In fact, elevated PBDE concentrations have been recorded in human breast milk. Due to the potential widespread exposure to PBDEs, this study investigates human blood concentrations of PBDEs generated through the 2003-2004 National Health and Nutrition Examination Survey. Through the use of statistical modeling, a comparison of mean …

Advanced Treatment Technologies For Mitigation Of Nitrogen And Off-Flavor Compounds In Onsite Wastewater Treatment And Recirculating Aquaculture Systems, Laura C. Rodriguez-Gonzalez

USF Tampa Graduate Theses and Dissertations

Non-point sources (NPS) of pollution are non-discernable, diffuse sources of pollution that are often difficult to localize and in turn mitigate. NPS can include stormwater runoff, agricultural/aquaculture wastes and wastes from small decentralized wastewater treatment systems, such as conventional septic systems. The mitigation of these NPS is imperative to reduce their potential detrimental effects on the water environment. This dissertation addresses novel treatment technologies for the mitigation of nutrients, particularly nitrogen, in Recirculating Aquaculture Systems (RAS) and onsite wastewater treatment systems (OWTS). The removal of trace organics limiting RAS production and water reuse were also investigated.

The first question this …

It’S Not All Sunflowers And Roses At Home: A Narrative Inquiry Of At-Risk Girls And Their Perceptions Of Their Educational Experiences, Jessica Aggeles Curtis

USF Tampa Graduate Theses and Dissertations

The population of girls being incarcerated continues to grow amid the decline of boy offenders. While society has seen an increase in violent offenses perpetrated by these girls, the majority are still detained on status offenses or for non-violent crimes. For many girls, school is a place to seek solace and safety. Yet, for a growing population of girls, the bond they once shared with school is disintegrating. This disconnect has proven to be a critical turning point in their lives. The purpose of this study is to give girls who have been removed from the general education setting a …

Psychosocial Mechanisms Of Outcome In Pediatric Psychiatry, Alessandro S. De Nadai

USF Tampa Graduate Theses and Dissertations

Nearly half of all youths experience a mental health disorder at some point during childhood (Merikangas et al., 2010). Pediatric psychopathology is associated with a substantial amount of impairment in the school, social, and home domains, and such symptoms can have adverse impacts on subsequent development (Beauchaine & Hinshaw, 2013; Patel, Flisher, Hetrick, & McGorry, 2007). Fortunately, a number of medications have demonstrated efficacy in treating a number of mental health conditions (Martin, Scahill, & Kratochvil, 2010). Despite these demonstrated effects, treatment response is often incomplete, and the mechanisms by which pharmacotherapy lead to behavior change are not well understood. …

Chemical Investigations Of Fungal Natural Products For Drug Discovery, Danielle H. Demers

USF Tampa Graduate Theses and Dissertations

Natural products have, historically, played an important role in drug discovery. Nevertheless, drug resistance, pathogen evolution, and global climate change threaten human health and nearly all current anti-infective treatments on the market today. It is undeniable that new drug discovery efforts are needed with increasing urgency.

Bolstered by a rich history of discovering treatments in the world around us, natural products chemists continue to look to the environment with increasing understanding and emerging technologies that allow efficient, effective isolation of new chemical entities. This thesis will describe one such endeavor.

Focusing on fungal natural products, herein is described the isolation …

Beyond Age And Stage: Consequences Of Individual Variation In Demographic Traits, Erin Elizabeth Feichtinger

USF Tampa Graduate Theses and Dissertations

Within natural populations, individuals vary in their propensity to grow, survive and produce offspring. Additionally, fates of individuals are often correlated and non-independent as a result of shared genes, rearing environment or both. Variation in demographic traits can change population dynamics over time. The effects of variation in individual growth rate on population growth rate, net reproductive rate and generation time are examined, along with quantifying the sources of variation in survival of a long-lived cooperatively breeding bird, the Florida Scrub-Jay (Aphelocoma coerulescens). I built a 2 stage population model with two growth phenotypes, fast and slow, and …

Traumatic Brain Injury In Adolescence: The Relationship Between High School Exiting And Future Productivity, Jeffrey S. Garofano

USF Tampa Graduate Theses and Dissertations

Traumatic brain injury (TBI) is the leading cause of death and disability among children and adolescents. Brain injury survivors are often left with persistent impairments that have the potential to impede daily functioning, delay or prevent the attainment of developmental milestones, and subsequently limit future productivity in adulthood. A shared goal of both neurorehabilitation and the educational system is to prepare youth for a productive adulthood with both systems of care having substantial, yet independent, literature bases regarding factors associated with productivity (e.g., engagement in employment or post-secondary education). It is currently assumed that because type of high school exiting …

Performing Narrative Medicine: Understanding Familial Chronic Illness Through Performance, Alyse Keller

USF Tampa Graduate Theses and Dissertations

This study presents the process of creating a performance ethnography of my family’s narratives about familial chronic illness and disability. I label this process performing narrative medicine. By documenting and granularly analyzing the process of my performance ethnography, the following chapters provide a step-by-step discussion of how families communicate about chronic illness/disability through storytelling and humor, and how/what performance does as a method, metaphor and object of study to further our current communicative practices and understandings of chronic illness and disability in families. I argue that performing narrative medicine is a heuristic for families living with chronic illness and …

The Role Of Elevated Hyaluronan-Mediated Motility Receptor (Rhamm/Hmmr) In Ovarian Cancer, Stephanie T. Buttermore

USF Tampa Graduate Theses and Dissertations

Ovarian cancer (OC) has the highest mortality among gynecological cancers. The high mortality is associated with the lack of an accurate screening tool to detect disease in early stage. As a result the majority of OCs are diagnosed in late stage. Further, the molecular events responsible for malignant transformation in the ovary remain poorly understood. Consequently, delineating key molecular players driving OC could help elucidate potential diagnostic, prognostic and therapeutic targets.

Receptor for hyaluronan-mediated motility (RHAMM) belongs to a group of hyaladherins, which share a common ability to bind to hyaluronan (HA). Intracellularly, RHAMM is involved in microtubule spindle assembly …

Bisphosphonate Functionalized Gold Nanoparticles For The Study And Treatment Of Osteoporotic Disease, Christopher Conners

USF Tampa Graduate Theses and Dissertations

The use of nanoparticles for disease treatment is an increasingly popular area of research. The potential for multi-functionality allows nanoparticles to be used as transport and delivery vehicles for drugs and as diagnostic aides, among other applications, to address the unmet needs of many disease treatments. One such class of disease is osteoporosis including severe disorders, like Paget’s disease, Osteogenesis Imperfecta and Legg Calve Perthes disease. In this dissertation, we discuss a nanoparticle system consisting of gold nanoparticles surface functionalized with primary amine bisphosphonates, which is a classification of pharmaceuticals that is common in the treatment of osteoporosis. Functionalized nanoparticles …

Role Of Amylase In Ovarian Cancer, Mai Mohamed

USF Tampa Graduate Theses and Dissertations

Ovarian cancer (OC) accounts for 4% of all cancer cases and 4.2% of all cancer deaths worldwide. OC is the most lethal gynecological cancer because it lacks early disease symptoms and does not have a specific diagnostic marker. As a result, more than 70% of OC patients are diagnosed in later stages when the disease has already metastasized and the 5-year survival rate has decreased to less than 20% compared with approximately 90% survival for women diagnosed with early stage disease. Therefore, I initiated my studies with a computational analysis of the 27 most commonly reported literature-derived ovarian cancer (LDOC) …

Health Literacy, Depression, Anxiety, And Posttraumatic Stress Disorder As Predictors Of Biological Markers Of Immune Functioning In Youth And Young Adults With Hiv, Courtney A. Lynn

USF Tampa Graduate Theses and Dissertations

Human immunodeficiency virus (HIV) is a virus that affects the body’s immune system (Centers for Disease Control [CDC], 2016a). HIV is a worldwide epidemic and disproportionately impacts youth in the United States. Youth living with HIV (YLWH) face significant mental health problems, namely depression, anxiety, and posttraumatic stress disorder (PTSD) with rates of these disorders discrepant from those in the general population. In addition to psychological difficulties, health literacy is another factor that influences individuals with HIV and is a priority of research with adolescents (Kalichman et al., 2000; Manganello, 2008). The National HIV/AIDS Strategy: Update 2020 (White House Office …

A Motivational Interviewing Intervention To Increase Utilization Of Smoking Cessation Services Among Veterans Undergoing Substance Use Treatment, Nicole S. Menzie

USF Tampa Graduate Theses and Dissertations

Smoking remains the single most preventable cause of death worldwide and primary cause of several types of cancer, cardiovascular disease, and respiratory illness (USDHHS, 2014). Although the prevalence of smoking among the general population continues to decline, the prevalence of smoking among the veteran population remains high. In addition, the prevalence of smoking among veterans with substance and alcohol use disorders is 2 to 3 times higher than in the general population. Over the years, the VA has implemented empirically-based treatments for smoking cessation to address the cigarette smoking epidemic. These services, however, are greatly underutilized. Motivational interviewing (MI) has …

Discovering Antibacterial And Anti-Resistance Agents Targeting Multi-Drug Resistant Eskape Pathogens, Renee Fleeman

USF Tampa Graduate Theses and Dissertations

Antibiotic resistance has been a developing problem for mankind in recent decades and multi-drug resistant bacteria are now encountered that are resistant to all treatment options available. In 2014, the World Health Organization announced that this problem is driving us towards a “post-antibiotic era” that will change the face of modern medicine as we know it. If lack of novel antibiotic development and FDA approval continues, by the year 2050, 10 million people will die each year to an antimicrobial resistant bacterial infection. With lack of pharmaceutical industry involvement in developing novel antibiotics, the responsibility now lies within the academic …

On Axon-Axon Interaction Via Currents And Fields, Aman Chawla

USF Tampa Graduate Theses and Dissertations

In this dissertation, we investigate coupling between axons in a tract, when the tract has an arbitrary cross-section, with the coupling being mediated by currents as well as electric fields. Under the current mediated setting, we develop a new master equation which captures the relative axonal geometry, specifically, the axon inclinations θ_i and the inter-axon distances W_ip and perform a number of simulations. We observe synchronization in our simulations of axons with differing diameters and separation-dependent coupling delays in the case of non-trivial tract geometries. For the field-mediated interaction setting, we determine the electric near-field of a firing …

Diversifying Homogenous Au(I)-Catalysis Through New Reaction Discovery, Stephen Motika

USF Tampa Graduate Theses and Dissertations

Homogenous Au(I)-catalysis has become a valuable synthetic tool to activate a host of unsaturated carbon functional groups towards nucleophilic addition. Over the course of the past two decades, many have embarked on new journeys within this field. Notably, the advancements in this field hinge on the development of new ligand systems that impart novel reactivity at the metal. Our group has focused on this area, as we have successfully demonstrated the utility of 1,2,3-triazoles as ligands for gold and a host of other transition metals and Lewis acids. With respect to gold catalysis, these ligands enhance the stability of the …

Bayesian Artificial Neural Networks In Health And Cybersecurity, Hansapani Sarasepa Rodrigo

USF Tampa Graduate Theses and Dissertations

Being in the era of Big data, the applicability and importance of data-driven models like artificial neural network (ANN) in the modern statistics have increased substantially. In this dissertation, our main goal is to contribute to the development and the expansion of these ANN models by incorporating Bayesian learning techniques. We have demonstrated the applicability of these Bayesian ANN models in interdisciplinary research including health and cybersecurity.

Breast cancer is one of the leading causes of deaths among females. Early and accurate diagnosis is a critical component which decides the survival of the patients. Including the well known ``Gail Model", …

Understanding The Experience Of Early-Onset Bipolar Disorder: A Phenomenological Study Of Emerging Adults, Kristin M. Smyth

USF Tampa Graduate Theses and Dissertations

While early-onset bipolar disorder (EOBD) has increased in prevalence, much remains to be understood about its phenomenology. Research and treatment models remain rooted in neurobiological conceptualizations of the illness that borrow heavily from models for the traditional adult-onset form of bipolar disorder. This study utilized a transcendental phenomenological design as a first step in obtaining an understanding of the lived experience of EOBD. A purposive sample of eight participants ages 18-25 participated in semi-structured interviews that elicited information on experiences of EOBD symptomatology and course of illness, stigma, experience with healthcare and treatment, and impact on identity, interpersonal relationships, and …

Structure And Activity Of Metallo-Peptides, Christian C. Tang

USF Tampa Graduate Theses and Dissertations

Metal ions are ubiquitously found in all living systems and play vital roles in supporting life forms by performing an array of biological activities. Such biological activities include binding and transforming organic molecules, and also acting as active centers and cofactors for catalysis of various acid-base and redox reactions in biological system. The main focus in bioinorganic chemistry is to elucidate the structural and functional roles of metals in biological systems. Among all transition metal ions, Cu²⁺ and Fe³⁺ are especially versatile and important due to their abilities to go through redox efficiently.

This dissertation can be divided …