Digital Commons Network^™

Design And Delivery Of Synthetic Mrna By A Peptide Nanoparticle, John H. Lockhart

USF Tampa Graduate Theses and Dissertations

The field of synthetic mRNA therapeutics is a rapidly expanding arm of gene therapies. The use of mRNA provides multiple benefits over viral or DNA vectors. synthetic mRNA vectors are immediately translated into protein after entering the cytoplasm of cells in contrast to DNA vectors that must first be transcribed to mRNA in the nucleus. This allows synthetic mRNA to produce a therapeutic protein in any cell type, including non-dividing cells. In addition, the non-replicative nature of mRNA means that insertional mutagenesis or generation of escape mutants is not a concern. However, the stimulation of innate immune responses by unmodified …

The Combined Effect Of Heat And Corona Charge On Molecular Delivery To A T-Cell Line In-Vitro, Molly A. Skinner

USF Tampa Graduate Theses and Dissertations

Gene therapy and immunotherapy are new up and coming fields in cancer treatment. In August of 2017, the first gene therapy was FDA approved called CAR-T cell therapy("FDA approval brings first gene therapy to the United States," 2017). This therapy uses the patient’s

own T-cells that are genetically modified to attack the cancerous cells. Currently, it takes 4-6 weeks to genetically modify these T-cells, which can be the difference between life and death for a cancer patient (Cheng, Jun, Jiang, & Xi, 2017). There is evidence that gene electrotransfer can help expedite the genetic modification process by having an easy …

Remembrance Of A Wound: Ethical Mourning In The Works Of Ana Menéndez, Elías Miguel Muñoz, And Junot Díaz, José Aparicio

USF Tampa Graduate Theses and Dissertations

Remembrance of a Wound: Ethical Mourning in the Works of Ana Menéndez, Elías Miguel Muñoz, and Junot Díaz explores Latinx experiences of ethical mourning, an act akin to a scar remaining after a wound heals. This literary ethical mourning respects the memories of people and places no longer present. I define ethical mourning in Derridean terms and connect it to testimonio to illustrate how certain Cuban American and Dominican American characters, having lost their homeland through exile, immigration, and political turmoil, become practiced at mourning. For Derrida, ethical mourning employs poetic language to bear witness to a loss in such …

Design And Implementation Of Degenerate Qpcr/Qrt-Pcr Primers To Detect Microbial Nitrogen Metabolism In Wastewater And Wastewater-Related Samples, Ryan F. Keeley

USF Tampa Graduate Theses and Dissertations

Nitrogen cycling processes can be tracked using quantitative Polymerase Chain Reaction (qPCR) to determine the presence and qReverse Transcriptase-PCR (qRT-PCR) to determine expression of key genes, or ‘biological markers’, for nitrogen metabolism. Nitrification is catalyzed in part, by two enzymes: ammonia monooxygenase (AMO; NH3 NH2OH) and nitrite oxidoreductase (NXR; NO2- NO3-). For denitrification, four enzymes act sequentially: nitrate reductase (NAR/NAP; NO3- NO2-), nitrite reductase (NIR; NO2- NO), nitric oxide reductase (NOR; NO  N2O), and nitrous oxide reductase (NOS; N2O  N2). A principle of wastewater treatment (WWT) is to remove excess nitrogen by taking advantage of natural nitrogen cycling …

Human Rights, Emotion, And Critical Realism: Proposing An Emotional Ontology Of International Human Rights, Ben Luongo

USF Tampa Graduate Theses and Dissertations

This dissertation proposes a critical realist framework of human rights and argues that emotion plays a foundational role in constituting a human rights ontology. I build this framework as a critical response to other IR human rights theories which have largely been developed in accordance with either empiricist or rationalist paradigms. Both empiricist and rationalist theories fail to articulate a firm ontological foundation which can support their human rights claims.

This ontological concern may not seem too important for human rights scholars interested in more substantive political issues, but it does lead to problems because no human rights theory is …

On Her Own: A Qualitative Study On The College-To-Career Transition Of Black Second-Generation Alumnae, Ladessa Y. Mitchell

USF Tampa Graduate Theses and Dissertations

The purpose of this qualitative study was to describe the college-to-career transition of Black second-generation alumnae in the development phase of emerging adulthood using Schlossberg’s (2011) Transition Model. As the researcher, I collected data from Black second-generation alumnae of predominantly White public universities in Florida to examine how their intersecting identities (i.e., race, gender, and educational status) and use of metaphorical capital (i.e., social, cultural, and human capital) influence their transition. The conceptual framework for this study is based on the 4 S’s of Schlossberg’s Transition Model as well as emerging adulthood, forms of capital, and the intersecting identities of …

Hybrid Fusion Protein For Inhibition Of Multiple Proteases For Chronic Wound Healing, Graham L. Strauss

USF Tampa Graduate Theses and Dissertations

Many diseases display a multitude of relevant factors that contribute to the persistence of the disease and difficulty treating it. The multifactorial characteristics of some diseases lead to the requirement of combination of treatments in order to restore health. The latter may necessitate the mixing of treatments, medications, and therapeutics to first halt the disease, then assist the human body in returning itself to a state of normality. For example, chronic wounds exhibit this multifactor characteristic in which there exist many factors that lead to the body’s inability to properly heal in a timely manner. This presents a further threat …

Character Strengths Of Ninth Grade Students In Accelerated Curricula: A Mixed-Methods Investigation, Hannah L. Gilfix

USF Tampa Graduate Theses and Dissertations

Students in accelerated curricula tend to have greater stress when compared to students in general education (Suldo, Shaunessy, & Hardesty, 2008). It is important for stakeholders to be able to help these students reach their goals and attain happiness. One potential method to help these students is to attend to their character strengths. People who effectively utilize their character strengths have achieved numerous positive outcomes including greater levels of well-being, self-esteem, and positive affect (Proctor, Malby, & Linley, 2011; Quinlan, Swain, Cameron, & Vella-Brodrick, 2014; Wood, Linley, Maltby, Kashan, & Hurling, 2011). Unfortunately, there is a lack of research on …

Mitigating Barriers To Chronic Disease Risk Factor Prevention And Management In Disadvantaged Communities, Krys M. Johnson

USF Tampa Graduate Theses and Dissertations

Background: The incidence and prevalence of chronic disease (CD) has increased in recent decades due to the advent of CD management and life-extending technologies. To address this burden on the population and healthcare system, evidence-based CD prevention programs have been developed to reduce the incidence and therefore the prevalence of these diseases. Despite the development and dissemination of effective interventions, African-Americans and Hispanics have disproportionately higher prevalence of CD and associated risk factors and disproportionately lower participation in CD prevention programs. Overweight/obesity and CDs may have intergenerational effects, with overweight adults being more likely to have overweight children who are …

An Insight Into The Biological Functions, The Molecular Mechanism And The Nature Of Interactions Of A Set Of Biologically Important Proteins., Adam A. Aboalroub

USF Tampa Graduate Theses and Dissertations

Characterization of protein structural properties is crucial to determine its role in normal and pathological conditions. In this dissertation, we have employed NMR spectroscopy in a combination of other biochemical and biophysical techniques to investigate the catalytic function, the molecular mechanism, and nature of the interactions of bmAANAT3, Cdc37, and TIMP2, respectively.

In the first project, we have employed an arylalkylamine N-acetyltransferases (AANATs) from Bombyx mori (bmAANAT3) to determine the role of the acetyl-group in coordinating the catalytic cycle in this family of enzymes. We have found that the occupancy of the acetyl-moiety in the catalytic funnel of these enzymes …

Understanding The Mechanisms Between Job Stress And Employee Sleep: A Daily Diary Study, Marijana L. Arvan

USF Tampa Graduate Theses and Dissertations

The present study investigated how three job stressors, workload, unfinished tasks, and mistreatment from coworkers and supervisors, manifest in sleep impairment. Using the transactional model of stress, allostatic load model, the cognitive activation theory of stress (CATS), and findings from the sleep medicine literature, each job stressor was predicted to have three distinct pathways to sleep quality: an affective path (anger or anxiety), a cognitive path (anticipatory stress or rumination), and a behavioral path (sleep hygiene behaviors). The proposed models argued for two-stage mediation, in which the cognitive and affective strains of job stressors impact sleep quality both directly and …

Intrinsically Disorder Proteins And Liquid-Liquid Phase Transitions In Neurodegenerative Diseases, April Lynn Darling

USF Tampa Graduate Theses and Dissertations

Neurodegenerative diseases have a negative impact on health and economics, effecting more than 20 million people in the United States and costing around $800 billion in 2018. Additionally, most neurodegenerative diseases such as Alzheimer’s Disease (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal Dementia (FTD), and several others are fatal and have no cure or effective treatment. Many of these diseases have a common theme of protein aggregates being present in disease tissue. These aggregates are thought to be toxic to the cell and prevention of pathological aggregation and elimination of these cellular aggregates may serve as a potential therapeutic approach to …

Novel Strategies On Characterizing Biologically Specific Protein-Protein Interaction Networks, Bi Zhao

USF Tampa Graduate Theses and Dissertations

The function, behavior, and environmental response of biological systems are essentially determined by the complex interaction and regulation of biomolecules inside the systems. Therefore, it is critical to characterize the inter-molecular interaction and regulation of biomolecules inside these systems. In this direction, many experimental techniques have been developed and these techniques have been used in many different model systems under various conditions. Consequently, a massive amount of data has been generated. These data cover multiple aspects of molecular interaction and regulation, such as protein-protein interaction, microRNA-RNA interaction, gene expression profiles, etc. While carrying rich information, these data may also contain …

Ethnic Identity As A Protective Factor In Early Adolescent Youth Depression: An Investigation Of Differences By Race And Gender, Leah Bonilla

USF Tampa Graduate Theses and Dissertations

Adolescent mental illness is a major concern in the Unites States. The adolescent stage is a critical developmental period of physical and mental changes, thus it is important to understand protective factors associated with positive wellbeing. The current study aimed to explore: (a) the associations among race, gender, ethnic identity, and depressive symptoms among eighth grade adolescents, (b) to what extent are there differences in degree of depressive symptoms among youth based on race and gender, and (c) to what extent a strong sense of ethnic identity serves as a protective factor against the development of depression among youth with …

Design And Synthesis Of Novel Agents For The Treatment Of Tropical Diseases, Linda Corrinne Barbeto

USF Tampa Graduate Theses and Dissertations

Infectious diseases have had an impact on populations since the beginning of humanity. Despite huge advances in the fight against these conditions, they continue to be the leading cause of death and disability for a large portion of the world’s populace. Those most adversely affected predominately live in the more improvised areas of the developing world. Often treatments are made inaccessible to these areas due to cost, complicated administration requirements, severe side effects, and / or compound instability. Even when treatments are available the accelerated development of resistance has created an urgent need for the creation of new molecules with …

Stable Isotope Geochemistry Of Shelled Marine Invertebrates: Wide-Ranging Applications, Nasser M. Al-Qattan

USF Tampa Graduate Theses and Dissertations

Mollusks grow by adding discrete growth layers throughout their lifetime (i.e., accretion). More specifically, most marine mollusks precipitate their shells in oxygen isotopic equilibrium with seawater or with a constant offset from equilibrium. The stable oxygen isotope values (δ¹⁸O) of their shells are determined by the temperature and δ¹⁸O values of the surrounding water during calcification without significant vital effects. In comparison, the stable carbon isotope values (δ¹³C) of their shells reflect those of the dissolved inorganic carbon (δ¹³C- DIC) and respired carbon dioxide. Therefore, variations in the oxygen and carbon isotope …

Targeting Pten For Therapy In Cancer And Ptenopathies, Emily Palumbo

USF Tampa Graduate Theses and Dissertations

PTEN, a dual protein and lipid phosphatase, regulates a myriad of cellular functions including PI3K pathway signaling, cell migration, proliferation, invasion and apoptosis. PTEN mutations often lead to multiple malignancies, including prostate, breast, endometrial, skin and brain cancers, associated with hyperactive PI3K signaling. PTEN mutations have also been associated with a variety of other diseases, classified as PTEN Hamartoma Tumor Syndromes (PHTS). In addition, compromised function or reduced expression of PTEN due to non-genomic mechanisms are associated with many types of hyperproliferative diseases, such as restenosis and neoplastic diseases, including melanoma, lung, breast, prostate and colon cancers. Although PI3K pathway …

The Tampa Gym Study: An Ethnographic Exploration Of Gyms, Female Gym-Goers And The Quest For Fitness In Tampa, Fl, Danielle Reneé Rosen

USF Tampa Graduate Theses and Dissertations

The Tampa Gym Study was an ethnographic examination of veteran women exercisers, their workout routines, and their attitudes towards the workouts that they undertake in two Tampa area gyms. The study’s principle objective was to study “fitness culture” in these facilities and the manner in which that culture is embodied in the language women use to describe themselves and their exercise behaviors.

The obesity crisis in the United States has been significantly responsible for an increase in membership in gyms and fitness facilities nationwide. The “culture of fitness” as it is embodied in these facilities has impacted women and their …

Prejudice Asymmetry: The Cultural Acceptance Of Sexism, Sophie Kuchynka

USF Tampa Graduate Theses and Dissertations

Sexism tends to be a culturally accepted form of prejudice. I propose the relatively strong trivialization of societal sexism stems from the unique benefits that men receive from the gender status hierarchy, compared to other types of group-based hierarchies. Three studies examined why people, men in particular, trivialize or justify gender bias in relation to other types of group-based biases. Study 1 was a correlational study that examined whether participants downplay the existence and social harm of gender bias in relation to racial, religious, and sexual orientation bias, moderated by participant gender. Participants reported stronger trivialization and denial of gender …

The Left Behind Generation: Instructional Practices To Increase The Technological Literacy Of Older Adults, Daphne Pace Phillips

USF Tampa Graduate Theses and Dissertations

This study sought to explore the utilization and perception of best practices by community-based technology training programs when instructing older adults to become technologically literate. The target population included adults age 55 years and older of the Baby Boomer generational cohort who ranged academically from possessing a high school diploma or General Education Diploma (GED) through a college degree and had enrolled in a local technology training program to improve their technological skill level with the goal of obtaining employment and/or to remain functionally independent. This study was conducted at three community technology training centers located in the southeast that …

Mhealth Elisa System For Point-Of-Care, Arsenii Zhdanov

USF Tampa Graduate Theses and Dissertations

Enzyme-linked immunosorbent assay (ELISA) is one of the most important technologies for biochemical analysis critical for diagnosis and monitoring of many diseases. Traditional systems for ELISA incubation and reading are expensive and bulky, thus cannot be used at pointof- care or in the field. Here, we design and demonstrate a new miniature mobile phone based system for ELISA. This mHealth system can be used to complete all steps of the assay, including incubation and reading. It can be fabricated at low cost, portable, and can transfer test results via mobile phone. We have designed the sample dispensing system, incubation chamber, …

Home-Based Cognitive Monitoring: The Role Of Personality And Predictors Of Adherence And Satisfaction, Nasreen A. Sadeq

USF Tampa Graduate Theses and Dissertations

Over the last several decades, a growing awareness of the benefits of regular screening for common health conditions, such as cancer and cardiovascular diseases, has paved the way for preventative screenings to become routine in medical settings. Given that cognitive impairment is frequently reported as the number one worry of older adults, home-based cognitive monitoring may be an innovative solution that allows middle aged and older adults to take an active role in monitoring an important aspect of their health. Although several home-based cognitive monitoring programs have been validated for use in clinical and home-based settings, the Cogstate Brief Battery …

Modulations Of Lipid Membranes Caused By Antimicrobial Agents And Helix 0 Of Endophilin, Nawal Kishore Khadka

USF Tampa Graduate Theses and Dissertations

Understanding the cellular membrane interaction with membrane active biomolecules and antimicrobial agents provides an insight in their working mechanism. Here, we studied the effect of antimicrobial agents; a recently developed peptidomimetics E107-3 and colistin as well as the N-terminal helix H0, of Endophilin A1 on the lipid bilayer.

It is important to discern the interaction mechanism of antimicrobial peptides with lipid membranes in battling multidrug resistant bacterial pathogens. We study the modification of structural and mechanical properties with a recently reported peptidomimetic on lipid bilayer. The compound referred to as E107-3 is synthesized based on the acylated reduced amide scaffold …

Clinging In Plethodontid Salamanders, Mary Kathleen O'Donnell

USF Tampa Graduate Theses and Dissertations

The ability to cling to and climb on inclined, vertical, and inverted surfaces gives animals access to additional shelter and food and may provide refuge from unsuitable temperature and moisture conditions or escape from ground-dwelling predators. Salamanders have been shown to cling to surfaces and engage in climbing behavior, but their maximum clinging capability and mechanisms of attachment are not well understood. While some arboreal salamanders possess prehensile tails, salamanders lack many morphological adaptations for attachment, such as claws, toe pads, and fibrillar adhesive pads. Maximum cling performance and surface area of attachment were measured, and the adhesive capability of …

Roux-En-Y Gastric Bypass Attenuates Obesity-Related Liver Injury, Drew Alan Rideout

USF Tampa Graduate Theses and Dissertations

Obesity is a growing epidemic in the United States with significant co-morbidities. Non-Alcoholic Fatty Liver Disease (NAFLD) is a prevalent manifestation of obesity that can lead to cirrhosis. Roux-en-Y gastric bypass (RYGB) results in substantial long-term weight loss and resolution of obesity-related metabolic diseases. There appears to be a weight-independent molecular mechanism for the improvement of diabetes mellitus and NAFLD after RYGB, which is poorly understood. Obesity is associated with chronic inflammation that accompanies the hepatic steatosis. Through unknown mechanisms, RYGB in humans increases serum levels of the fat-derived adipocytokine, adiponectin. Adiponectin (an anti-inflammatory cytokine) is known to have a …

A Small Rna And Dna Binding Protein Contribute To Biofilm Development In Bartonella Henselae, Udoka Okaro

USF Tampa Graduate Theses and Dissertations

A biofilm, which is associated with 80% of chronic infections in humans, is formed when bacteria aggregate, attach to a substrate and secrete a matrix protecting the bacteria from host cell defenses and antibiotics. Bartonella henselae (B. henselae) is the causative agent of cat scratch disease, persistent bacteremia, and one of the most frequently reported causes of blood-culture negative endocarditis (BCNE) in patients. The ability of B. henselae to adhere to the heart valve, form a biofilm and vegetation to cause endocarditis increases the morbidity and mortality rate in infected patients. The presence of a trimeric autotransporter adhesin (TAA) called …

A Machine Learning Approach To Predicting Community Engagement On Social Media During Disasters, Adel Alshehri

USF Tampa Graduate Theses and Dissertations

The use of social media is expanding significantly and can serve a variety of purposes. Over the last few years, users of social media have played an increasing role in the dissemination of emergency and disaster information. It is becoming more common for affected populations and other stakeholders to turn to Twitter to gather information about a crisis when decisions need to be made, and action is taken. However, social media platforms, especially on Twitter, presents some drawbacks when it comes to gathering information during disasters. These drawbacks include information overload, messages are written in an informal format, the presence …

Risk And Resilience Pertaining To Mental Health Of Youth Attending A College In Kathmandu Who Were Exposed To The 2015 Earthquakes During Their Adolescence: A Mixed Method Study, Vinita Sharma

USF Tampa Graduate Theses and Dissertations

Background: Adverse mental health outcomes have been previously reported among survivors of natural disasters. Available studies mostly focus on risk factors and not resilience. In 2015, a series of earthquakes caused massive loss of lives and livelihood in Nepal. Epidemiological data pertaining to youth mental health in Nepal is limited. Information about the post-earthquake mental health of youth who were exposed to earthquakes during their adolescence in Nepal is scarce.

Purpose: The purpose of this study was to address risk and resilience factors related to mental health among college-attending Nepali youth (18-20 years) exposed to the 2015 earthquake during their …

Exploring New Teacher Beliefs: Identity, Home-Life, And Culture In The Classroom, Frederick B. Bradley Iii

USF Tampa Graduate Theses and Dissertations

A persistent shortage of science, technology, engineering, and math (STEM) students, teachers, and professionals is seen by many as a threat to the nation’s global economic standing. Deficits in these areas are often attributed to a lack of quality K-12 STEM education, which is due in large part to a high rate of teacher turnover. Moreover, such teacher attrition has been shown to occur far more often in high-need schools and districts; thus serving to further marginalize disadvantaged members of society.

This study occurs within the context of The Robert Noyce Scholarship Program at our research-intensive university in the southeastern …

“It’S A Broken System That’S Designed To Destroy”: A Critical Narrative Analysis Of Healthcare Providers’ Stories About Race, Reproductive Health, And Policy, Brianna Rae Cusanno

USF Tampa Graduate Theses and Dissertations

Constructions of race, reproductive health, and gender have been inextricably linked in the United States since the beginning of the nation. Today, these linkages remain evident in the marked racial and gender inequities in reproductive health outcomes that persist in the U.S. To better understand how these meanings and material outcomes are negotiated and produced by actors on the ground, this study asked: “How do reproductive healthcare providers (RHPs) communicate about the intersections of race, reproductive health, and policy?” I conducted semi- structures interviews with 24 RHPs, resulting in over 35 hours of recorded interviews. Drawing on critical-cultural communication, Reproductive …