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Cloning And Characterization Of Il-1Β, Il-8, Il-10, And Tnfα From Golden Tilefish (Lopholatilus Chamaeleonticeps) And Red Snapper (Lutjanus Campechanus), Kristina L. Deak

USF Tampa Graduate Theses and Dissertations

Cytokines are pleiotropic and redundant signaling molecules that govern the inflammatory response and immunity, a critical ecological parameter for organism success and population growth. Produced at the site of injury or pathogen intrusion by a variety of cell types, cytokines mediate cell-signaling in either an autocrine or paracrine manner. The type and magnitude of the cytokine milieu produced subsequently dictates the strength and form of immune response. As the most diverse vertebrate group, with a high sensitivity to contaminants, fish represent an important foci for the evaluation of immune system evolution, function, and alteration upon toxicant exposure. While many cytokines …

Residual-Based Variational Multiscale Les With Wall-Modeling For Oceanic Boundary Layers In Shallow Water, Roozbeh Golshan

USF Tampa Graduate Theses and Dissertations

Large-eddy simulation (LES) of wind and wave forced oceanic turbulent boundary layers is performed using the residual-based variational multiscale method (RBVMS) and near-wall modeling. Wind and surface gravity wave forcing generates Langmuir turbulence characterized by Langmuir circulation (LC) with largest scales consisting of streamwise vortices aligned in the direction of the wind, acting as a secondary flow structure to the primary wind-driven component of the flow. The LES here is representative of a shallow water continental shelf flow (10 to 30 meters in depth) far from lateral boundaries in which LC engulfs the full depth of the water column and …

Phenyl Functionalized Sol-Gel Silica Sorbent For Capillary Microextraction And Chromia-Based Sol-Gel Ucon Stationary Phase For Capillary Gas Chromatography, Michael M. Mclean

USF Tampa Graduate Theses and Dissertations

The first chapter of this thesis presents an introduction to sol-gel methodology whose usefulness as a synthetic route will be demonstrated with two applications in chromatography. The first application involves the fabrication of a capillary micro-extraction (CME) device by coating a phenyl functionalized extracting phase on the inner surface of a fused silica capillary for analyte pre-concentration. The device was coupled on-line to a RP-HPLC system and practicality was demonstrated using allergens as target analytes. The allergens chosen as model analytes are typically found in fragrance products and food. Most of the 26 fragrance allergens that are monitored by various …

Reliable Design And Operations Of Infrastructure Systems, Yu An

USF Tampa Graduate Theses and Dissertations

The reliability issue of the infrastructure systems has become one of the major concerns of the system operators. This dissertation is a collection of four published and working papers that address the specific reliable design and operations problems from three different application settings: transportation/telecommunications network, distribution network, and power plant. In these four projects, key random factors like site disruption and uncertain demand are explicitly considered and proper research tools including stochastic programming, robust optimization, and variants of robust optimization are applied to formulate the problems based on which the important and challenging modelling elements (nonlinear congestion, disruption caused demand …

Spatial Analysis Of Foreclosures In Hillsborough County, Brian Arthur Sandrock

USF Tampa Graduate Theses and Dissertations

This study examines the spatial impact various socio-demographic and housing factors might have in the foreclosure lis pendens rate within various Hillsborough County, Florida tracts as well as comparing those results with past research. Hopefully the techniques used in this study can be implemented elsewhere in order to better study the foreclosure crisis. The methods used within this research were chosen carefully in order to best understand what is being observed. One method is OLS regression which helps see the impact of each variable and if that impact has a negative or positive effect on the rate of foreclosure. Bivariate …

Analysis Of Advanced Supercritical Carbon Dioxide Power Cycles For Concentrated Solar Power Applications, Saeb Mostaghim Besarati

USF Tampa Graduate Theses and Dissertations

Solar power tower technology can achieve higher temperatures than the most common commercial technology using parabolic troughs. In order to take advantage of higher temperatures, new power cycles are needed for generating power at higher efficiencies. Supercritical carbon dioxide (S-CO₂) power cycle is one of the alternatives that have been proposed for the future concentrated solar power (CSP) plants due to its high efficiency. On the other hand, carbon dioxide can also be a replacement for current heat transfer fluids (HTFs), i.e. oil, molten salt, and steam. The main disadvantages of the current HTFs are maximum operating temperature …

Age And Gender Recognition For Speech Applications Based On Support Vector Machines, Hasan Erokyar

USF Tampa Graduate Theses and Dissertations

Automatic age and gender recognition for speech applications is very important for a number of reasons. One of the reasons is that it can improve human-machine interaction. For example, the advertisements can be specialized based on the age and the gender of the person on the phone. It also can help identify suspects in criminal cases or at least it can minimize the number of suspects. Some other uses of this system can be applied for adaptation of waiting queue music where a different type of music can be played according to the person's age and gender. And also using …

A Decision Support Model For Personalized Cancer Treatment, Florentino Antonio Rico-Fontalvo

USF Tampa Graduate Theses and Dissertations

This work is motivated by the need of providing patients with a decision support system that facilitates the selection of the most appropriate treatment strategy in cancer treatment. Treatment options are currently subject to predetermined clinical pathways and medical expertise, but generally, do not consider the individual patient characteristics or preferences. Although genomic patient data are available, this information is rarely used in the clinical setting for real-life patient care. In the area of personalized medicine, the advancement in the fundamental understanding of cancer biology and clinical oncology can promote the prevention, detection, and treatment of cancer diseases.

The objectives …

Analysis Of Water Quality For Lake Tarpon, Pinellas County, Florida, Kristi Nichole Savio

USF Tampa Graduate Theses and Dissertations

Urbanization has taken a major toll on the water quality of Lake Tarpon. In response, there have been management strategies and legislation put in place to help improve water quality. Our objective was to identify what management strategies and other environmental factors were driving changes in Lake Tarpons water quality from 1970 - 2010. Trends in water quality were analyzed against precipitation, land use and water quality management strategies to achieve this. Results found that two water quality management strategies, the creation of the Lake Tarpon Outfall Canal and the closing of the Lake Tarpon Sink, improved water quality the …

Exergetic Life Cycle Assessment Of Electrospun Polyvinylidene Fluoride Nanofibers, Salman Ali Abbasi

USF Tampa Graduate Theses and Dissertations

Assessing the sustainability of nanomanufacturing products and processes has been difficult to achieve using conventional approaches mainly due to an inadequate inventory, large process-to-process variation, and a dearth of relevant toxicology data for nanomaterials. Since these issues are long term in nature, it is required to create hybrid methodologies that can work towards filling the existing gaps. Merging thermodynamic techniques such as the exergy analysis with environmental assessments can help make better, more informed choices while providing an opportunity for process improvement by enabling to correctly quantify efficiency loss through the waste stream, and by locating the exact areas for …

Dynamics And Survival Of Coral And Octocoral Juveniles Following Disturbance On Patch Reefs Of The Florida Reef Tract, Lucy Bartlett

USF Tampa Graduate Theses and Dissertations

Over the past several decades, rapid decline in adult stony-coral (comprising the Orders Scleractinia and Anthomedusae, specifically Family Milleporidae) cover has occurred concurrent with an increase in adult octocoral (Octocorallia/gorgonian) cover along the Florida Reef Tract. In January 2010, the Florida Keys experienced extremely cold air and water temperatures, below the lethal threshold for many reef organisms including corals. Very high stony-coral mortality occurred on some patch reefs. The newly-available space created by this disturbance event provided the opportunity for recruitment and settlement of new coral larvae and other reef organisms.

The goal of this study was to examine post-disturbance …

Accessibility's Influence On Population Location Near Light Rail In The Denver Region, Christophe Michael Zuppa

USF Tampa Graduate Theses and Dissertations

Accessibility is the most important concept in transportation planning because it describes the ease of travel to opportunities vital for everyday needs. Theoretically, people locate closer to transit corridors if accessibility improves. One desired benefit from light rail is denser land use patterns in the form of Transit Oriented Development (TOD) that captures population growth. In October 1994, the City of Denver, CO, joined the list of American cities that have implemented light rail within the last 33 years. Since then, five corridors have opened there, and planners are retooling their zoning codes to allow TOD near light rail. The …

Groundwater Controls On Physical And Chemical Processes In Streamside Wetlands And Headwater Streams In The Kenai Peninsula, Alaska, Michael Kroh Callahan

USF Tampa Graduate Theses and Dissertations

For this dissertation I studied groundwater and surface water interactions in the Kenai Lowlands, Alaska. In particular, I examine two important aspects of groundwater and surface water interactions: 1) Groundwater's influence on surface-water temperature; and 2) Groundwater's role in forming hydrologic flow paths that can connect uplands to streamside wetlands and streams. Chapter 2 investigates the controls on stream temperature in salmon-bearing headwater streams in two common hydrogeologic settings: 1) drainage-ways, which are low-gradient streams that flow through broad valleys; and 2) discharge-slopes, which are high gradient streams that flow through narrow valleys. The results from chapter 2 showed significant …

Is More Always Better: Comparing The Effects Of Single And Multiple Learning Channels On Academic Performance, Samantha Ann Spillman

USF Tampa Graduate Theses and Dissertations

Precision teaching (PT) is a measurement system used in multiple settings for all types of behavior, from driving to mathematics. The ultimate goal of PT is to develop fluent, free operant behaviors through analyzing response frequencies on a standard celeration chart. Research has found PT to be effective at improving both the speed and accuracy of academic skills. There is little research, however, in the effects of learning channels, a component of PT, as they relate to the acquisition of academic skills. The present study examined the relationship between single and multiple learning channels on the acquisition of mathematics skills …

Assessments Of Surface-Pelagic Drift Communities And Behavior Of Early Juvenile Sea Turtles In The Northern Gulf Of Mexico, Robert F. Hardy

USF Tampa Graduate Theses and Dissertations

Knowledge of species distribution and habitat associations are essential for conservation measures. Such information is lacking for many marine species due to their occupancy of broad and ephemeral habitats that are difficult to access for study. Sea turtles, specifically the surface-pelagic juvenile stage of some species, are a group for which significant knowledge gaps remain surrounding their distribution and habitat use. Recent research has confirmed the long-standing hypothesis that the surface-pelagic juvenile stage occurs within surface-pelagic drift communities (SPDC). Within the North Atlantic and surrounding basins, the holopelagic macroalgae Sargassum spp. dominates SPDC and serves as a remotely-detectable indicator of …

Distribution Of Dissolved Trace Metals And Carbon System Parameters In The Surface Waters Of The Hillsborough River And Tampa Bay, Matthew Matthias Elliott

USF Tampa Graduate Theses and Dissertations

This work represents a first look at the general spatial distribution of trace metals and the inorganic carbon system in the surface waters of Tampa Bay. Tampa Bay's chemical environment bears many influences ranging from hydrological and geological to anthropogenic and meteorological. A large y-shaped estuary on the west-central side of the Florida Peninsula, Tampa Bay extends over 400 square miles and has a 2200 square mile watershed that includes extensive swamps, scrub, agricultural lands and densely urbanized areas. Reaching 37 miles from the northernmost point of Old Tampa Bay to the mouth of the estuary near the Sunshine Skyway …

Photophysical And Electronic Properties Of Low-Bandgap Semiconducting Polymers, Evan Lafalce

USF Tampa Graduate Theses and Dissertations

In this Ph.D. work, we investigate the optoelectronic properties of low-bandgap semiconducting polymers and project the potential for employing these materials in electronic and photonics devices, with a particular emphasis on use in organic solar cells. The field of organic solar cells is well developed and many of the fundamental aspects of device operation and material requirements have been established. However, there is still more work to be done in order for these devices to ultimately reach their full potential and achieve commercialization. Of immediate concern is the low power conversion efficiency demonstrated in these devices so far. In order …

Auxiliary Roles In Stt-Mram Memory, Jayita Das

USF Tampa Graduate Theses and Dissertations

Computer memories now play a key role in our everyday life given the increase in the number of connected smart devices and wearables. Recently post-CMOS memory technologies are gaining significant research attention along with the regular ones. Spin Transfer Torque Magnetoresistive RAM (STT-MRAM) is one such post-CMOS memory technology with a rapidly growing commercial interest and potential across diverse application platforms. Research has shown the ability of STT-MRAM to replace different levels of memory hierarchy as well. In brief, STT-MRAM possesses all the favorable properties of a universal memory technology. In this dissertation we have explored the roles of this …

Continuation In Us Foreign Policy: An Offensive Realist Perspective, Bledar Prifti

USF Tampa Graduate Theses and Dissertations

This dissertation is a study of US foreign policy that aims at maintaining its regional hegemonic status and preventing the emergence of another regional hegemon by implementing the offshore balancing strategy. US intervention during the 2003 Iraq War, strained US-Iran relationship, and the establishment of the Islamic State of Iraq and the Levant (ISIL) in early 2014 compel a reevaluation of US foreign policy. Two major claims of this dissertation include: (1) US foreign policy is consistent with offensive realist theoretical claims; and (2) US foreign policy is characterized by continuity when it comes to issues related to America's strategic …

Operation Optimization And Water Quality Simulation Of Potable Water Distribution System, Xiongfei Xie

USF Tampa Graduate Theses and Dissertations

A potable water distribution system (WDS) consists of pipes, pumps, valves, storage tanks, control and supporting components. Traditionally, it has two basic functions. First, provides end users with potable water at sufficient pressures and good water quality. Second, provides sufficient pressure and flow for fire fighting. Currently, potable water is still the least expensive material for fire fighting. To accomplish these two goals, water utilities have to consider the integrity and security of the water network. As a result, this research selected three research topics that are closely related to the daily operation of water utilities and water quality simulation. …

Using Mimic Methods To Detect And Identify Sources Of Dif Among Multiple Groups, Seokjoon Chun

USF Tampa Graduate Theses and Dissertations

This study investigated the efficacy of multiple indicators, multiple causes (MIMIC) methods in detecting uniform and nonuniform differential item functioning (DIF) among multiple groups, where the underlying causes of DIF was different. Three different implementations of MIMIC DIF detection were studied: sequential free baseline, free baseline, and constrained baseline. In addition, the robustness of the MIMIC methods against the violation of its assumption, equal factor variance across comparison groups, was investigated. We found that the sequential-free baseline methods provided similar Type I error and power rates to the free baseline method with a designated anchor, and much better Type I …

Crystal Engineering Of Functional Metal-Organic Material Platforms For Gas Storage And Separation Applications, Sameh Khamis Elsaidi

USF Tampa Graduate Theses and Dissertations

Metal-organic materials (MOMs) represent a unique class of porous materials that captured a great scientific interest in various fields such as chemical engineering, physics and materials science. They are typically assembled from metal ions or metal clusters connected by multifunctional organic ligands. They represent a wide range of families of materials that varied from 0D to 3D networks: the discrete (0D) structures exemplified by metal-organic polyhedra (MOPs), cubes and nanoballs while the polymeric 1D, 2D and 3D structures exemplified by coordination polymers (CPs). Indeed, the porous 3D structures include metal-organic frameworks (MOFs), porous coordination polymers (PCPs) and porous coordination networks …

An Analysis Of (Bad) Behavior In Online Video Games, Jeremy Blackburn

USF Tampa Graduate Theses and Dissertations

This dissertation studies bad behavior at large-scale using data traces from online video games. Video games provide a natural laboratory for exploring bad behavior due to their popularity, explicitly defined (programmed) rules, and a competitive nature that provides motivation for bad behavior. More specifically, we look at two forms of bad behavior: cheating and toxic behavior.

Cheating is most simply defined as breaking the rules of the game to give one player an edge over another. In video games, cheating is most often accomplished using programs, or "hacks," that circumvent the rules implemented by game code. Cheating is a threat …

Validation Of The Scores Of The Instructional Pedagogical And Instructional Student Engagement Components Of Fidelity Of Implementation, Sandra F. Naoom

USF Tampa Graduate Theses and Dissertations

Students cannot benefit from what they do not experience. Multiple reasons exist for why an intervention may not be delivered as it was designed. In this era of educational accountability and limited dollars to go around, understanding how an intervention is delivered in the classroom is key to understanding program outcomes. In order to assess whether a program has been implemented as intended, an assessment of fidelity is needed. However assessing fidelity is complex given varying conceptual interpretations, which then fosters inconsistent application of methods to measure the construct. Additionally the methods for validating fidelity measures are still unclear. The …

Science Teachers' Understandings Of Science Practices Before And After The Participation In An Environmental Engineering Research Experiences For Teachers (Ret) Program, Dilek Özalp

USF Tampa Graduate Theses and Dissertations

In 2012, National Research Council published a new science education framework that explains the science practices and its importance in understanding the process of knowledge development. The students were expected to engage in all the practices by grade 12. All science teachers need some kind of support to improve their understandings of these science practices (NRC, 2012). An important key component of engaging teachers in scientific investigations is to have the teacher participate in a research laboratory experience (NRC, 1996). Research Experiences for Teachers programs (RET) serve as a promising form of professional development to achieve this goal. These programs …

Managing Regional Water Resources Amidst Rapid Urbanization In Southwest Florida: A Case Study, Nicole Owusua Caesar

USF Tampa Graduate Theses and Dissertations

Experts and organizations involved in freshwater resources management have emphasized the importance of long-term urban resource planning and management that considers the tight coupling which exists between human - nature - technology systems. The resistance of contemporary urban growth efforts to consider resource carrying capacities and ecosystem requirements has led to costly unintended consequences including the deterioration of natural capital and their associated ecosystem services, and the degradation of water resource flows. As these problems continue to worsen, resource experts have called for the development of a new water resource management paradigm inclusive of various sustainability criteria.

Historically water-rich Florida …

Characterization Of The Depositional Environment Of Phreatic Overgrowths On Speleothems In The Littoral Caves Of Mallorca (Spain): A Physical, Geochemical, And Stable Isotopic Study, Liana Marie Boop

USF Tampa Graduate Theses and Dissertations

Phreatic overgrowths on speleothems (POS) are one of many sea-level proxies available to Quaternary geologists in Mallorca; these carbonate encrustations form at the air-water interface in cave passages flooded with brackish water. POS are ideal for reconstruction of western Mediterranean sea level because they are widespread in Mallorca's caves, can be precisely dated by U-series methods, constrain sea-level stands to sub-meter elevation, and are well preserved and accessible in the subterranean environment.

This research investigates the POS depositional environment, which is relatively understudied compared to the other proxies used for sea-level reconstructions. This disparity has led to assumptions on many …

Risk Of Compliance: Tracing Safety And Efficacy In Mef-Lariam's Licensure, Julie Marie Gerdes

USF Tampa Graduate Theses and Dissertations

The Walter Reed Institute of Army Research developed the antimalarial drug mefloquine then collaborated with Hoffman-La Roche to produce the drug under its brand name "Lariam," after Food and Drug Administration (FDA) approved licensure in 1989. For over twenty years, the Army used this pill as its "drug of choice" for soldiers deployed to endemic regions until 2009, and in 2013 the Food and Drug Administration warned that the drug's neurotoxic effects could be lasting, if not permanent. The sociopolitical exigence of developing a new biochemical antimalarial drug rushed the development and licensure processes, and the modern craving for certainty …

Opuntia Ficus-Indica Mucilage Potential To Remove Nuclear Active Contaminants From Water Based On A Surrogate Approach, Jose Adan Leon

USF Tampa Graduate Theses and Dissertations

Potable water is a necessity and unfortunately, it can be contaminated in many different ways. This paper specifically addresses the case scenario when, water has been contaminated by radionuclides. The mucilage of Opuntia ficus-indica (OFI), has been proven to be a great flocculating agent, turbidity reducer, crude oil dispersant and an environmentally friendly substance as an absorbent of water contaminants. In this work, the OFI mucilage was investigate to evaluate its capacity to separate radioactive ions from water. The surrogate approach is modeled based on water studies performed by Willi A. Brand, who proved that radioactive isotopes behave similar to …

Connecting Institutional Discourses And Everyday Understandings Of Climate Change: Viewpoints From A Suburban Neighborhood In Tampa, Florida, Christopher Metzger

USF Tampa Graduate Theses and Dissertations

Despite a general consensus regarding anthropogenic global climate change across the international scientific community, many of the major greenhouse gas producers in the world, especially the United States, are hesitant to implement strict emissions regulations. According to some prominent atmospheric scientists, such as James Hansen and Michael Mann, if industrialized countries continue to produce carbon emissions at current rates, an irreversible planetary tipping point of raising temperatures 2°C above pre-industrial levels could be reached in less than 40 years. Societies have a wealth of information from the natural sciences to understand the climate problem and currently possess the technological means …