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Natural Disasters And Human Capital: Empirical Evidence From Indonesia, Lei Lv

USF Tampa Graduate Theses and Dissertations

In developing countries, natural disasters could destroy physical capital and adversely affect human capital accumulation by disrupting individual decisions. Such decisions play a critical role in determining individuals' human capital accumulation process and have a lifelong effect on their happiness and economic prosperity. To better understand how natural disasters affect human capital in developing countries, this dissertation uses the earthquakes in Indonesia as a natural experiment to study how this earthquake affects health, child marriage, and education. For the first chapter, I study how the 2006 Yogyakarta Earthquake a affects water-related acute disease symptomsin the short and long run. By …

Spatial Pattern And Environmental And Social Factors Of Monkeypox In The Contiguous United States, Linbo Han

USF Tampa Graduate Theses and Dissertations

Monkeypox is a rare viral disease that is usually transmitted to humans through contact with infected animals, such as rodents or primates. Although its symptoms are similar to those of smallpox, monkeypox is generally less severe and rarely fatal. In recent years, however, there has been an uptick in the number of reported cases, with a large outbreak occurring in several parts of the world in May 2022. This has raised concerns among public health officials and researchers, who are working to better understand the factors that contribute to the spread of the disease. One promising approach to studying the …

Diatom Diversity And Functional Groups In 72 Florida Lakes: Assessing Ecological Changes For Improved Protection And Management, Francesca M. Lauterman

USF Tampa Graduate Theses and Dissertations

Florida lakes are diverse ecosystems and are an important part of the state's biodiversity and ecosystem services. As human population and development increase across the state, many lakes have been documented as nutrient impaired and are unable to maintain essential ecological functions. In this study, diatom diversity and functional groups in 72 Florida lakes were assessed for their relationships with associated water-quality data. Common alpha diversity indices, including Shannon-Weiner Index, the Gini-Simpson Index, and Hill’s numbers were utilized to measure trends in diatom communities, while the relationships between diatom lifeform characteristics and environmental data were explored using multivariate methods and …

Of Mētis And Cuttlefish: Employing Collective Mētis As A Theoretical Framework For Marginalized Communities, Justiss Wilder Burry

USF Tampa Graduate Theses and Dissertations

This LGBTQ+ rhetoric of health and medicine (RHM) community-based research explores the ways community members and queer community participants communicate about potential intimate partner selection in Central Florida. The dissertation introduces the theoretical framework of “collective mētis” as a way to analyze and discuss community communication against Florida’s required HIV disclosure laws that attempt to medicalize this and control this community’s intimate partner selection. Research questions include: 1) How do people in counterpublic enclaves (Chávez, 2011) communicate their potential intimate partner desires to others in the community including the types of disclosures they express and negotiate, and how? 2) How …

Utilizing Neoantigen-Specific Cd4* T Cells And Immune Checkpoint Modulation To Advance Adoptive Cell Therapy With Tumor-Infiltrating Lymphocytes For Metastatic Melanoma Patients, Maclean Scott Hall

USF Tampa Graduate Theses and Dissertations

Adoptive cell therapy (ACT) with tumor-infiltrating lymphocytes (TIL) is a promising immunotherapeutic approach for patients with advanced solid tumors. Metastatic melanoma is a tumor type amenable to immunotherapy in part due to the presence of antigen-specific TIL. These T cells can be activated and expanded for ACT, which has resulted in relatively high rates of clinical responses. While numerous advances have been made, the inclusion of neoantigen-specific CD4+ T cells within TIL infusion products remains underexplored and therefore offers a significant opportunity for progress. Similarly, immune checkpoint inhibitors, specifically PD-1 blocking antibodies, augment antitumor immunity and may improve the outcomes …

Population Demography, Spatial Ecology, And Habitat Use Of The Florida Box Turtle (Terrapene Bauri) On A Barrier Island, Michael D. Mills

USF Tampa Graduate Theses and Dissertations

Turtles are one the most threatened vertebrate groups in the world due to anthropogenic threats such as habitat loss and overexploitation. In addition to occupying a range that has been vulnerable to major habitat loss, the Florida box turtle (Terrapene bauri) is particularly at risk of overexploitation due to its popularity in the pet trade. Sanibel Island is a barrier island in southwest Florida that has experienced major habitat loss and is the site of a recent poaching event. In response to these threats, studies of both the population demography and spatial ecology were conducted on Sanibel’s Florida box turtle …

Evaluating The Effect Of Public Health Governance Structure And Public Opinion On Covid-19 Disease Control Interventions, Daniel Chacreton

USF Tampa Graduate Theses and Dissertations

Background

Since the start of the COVID-19 outbreak, the responses to the pandemic varied greatly from nation to nation. In the US, state-level variance in responses has likely contributed to the disparate COVID-19 infection-related state-level outcomes such as incidence and case fatality rates. The large variances in national and sub-national responses to COVID-19 when combined with the abundance of factors that influence disease transmission rates has created a number of important research opportunities.

Methods

In this vain, this research study aims to describe the relationship that exists between public health governance structure and public sentiment and COVID-19 control measure effectiveness …

An Examination Of Parental Coping Strategies During Catastophic Events, Vivian K. Mills

USF Tampa Graduate Theses and Dissertations

On March 11, 2022, the World Health Organization (WHO) declared the Sars CoV-2 a global pandemic. The virus that originated in Wuhan, China, had spread to several other countries causing governments to shut down and issue safety mandates to slow and stop the spread. The pandemic caused global changes to normal routines and highlighted the importance of coping during these tough times. Although the global COVID-19 pandemic encompassed much of the news during the past few years, it is not an isolated catastrophic event to have impacted society. Natural disasters, such as hurricanes and wildfires, wars, and other disasters have …

Anthropogenic Fecal Pollution Affects Microbial Water Quality And Multidrug Resistance In Tropical Aquatic Environments, Adriana González-Fernández

USF Tampa Graduate Theses and Dissertations

Although an often-overlooked issue, fecal pollution at recreational beaches should concerneveryone. Worldwide more than 80% of wastewater is discharged into rivers or the ocean without any treatment. Untreated and inefficiently treated wastewater can introduce diseasecausing microorganisms into the aquatic environment. Fecal-borne pathogens can also originate in animal waste, and humans can be exposed to these pathogens from poorly managed animal feces that make their way into the aquatic environment. Exposure to waterborne pathogens in recreational waters is a public health hazard, as it facilitates the transmission of waterborne illness, and surface waters serve as natural reservoirs of antibiotic resistant bacteria …

An Anthropology With Human Waste Management: Non-Humans, The State, And Matters Of Care On The Placencia Peninsula, Belize, William Alex Webb

USF Tampa Graduate Theses and Dissertations

The management of human waste is a seldom studied phenomenon in anthropology. Yet across the globe, in countries both rich and poor, it presents pervasive and difficult to tame problems. This dissertation draws on complimentary theories of management and entanglements to explore the practices and processes of organizing human waste on the Placencia Peninsula, Belize. The results illustrate how problems are conditioned and defined by messy relations between institutions, people, technologies, materials, and ecological life.

Fieldwork and analysis for this work was a culmination of years of interdisciplinary collaboration between other anthropologists and engineers at the University of South Florida. …

Seroprevalence Of Sars-Cov-2 Antibodies, The Gut Microbiome, And Peripherally Circulating Cytokines In Pregnant Hispanic Females, Tina Susanna Mutka

USF Tampa Graduate Theses and Dissertations

Pregnant Hispanic females have been disproportionately impacted by SARS-CoV-2 andhave experienced high rates of asymptomatic or mildly symptomatic, unrecognized infection. Acute SARS-CoV-2 infection has been associated with gut microbiome changes and altered peripherally circulating cytokine levels in symptomatic, non-pregnant individuals.

This study investigated the seroprevalence of anti-SARS-CoV-2 IgG antibodies in a sample of pregnant Hispanic females (n = 197). A sub-sample of SARS-CoV-2 seropositive and seronegative pregnant Hispanic females were followed across pregnancy and their bacterial gut microbiome was characterized, and cytokine levels measured to investigate relationships between SARS-CoV-2 serostatus, the gut microbiome, and peripherally circulating cytokine levels across pregnancy. …

Japan’S Covid 19 Infection Rate: A Focus On Tokyo Neighborhoods, Lauren Koerner

USF Tampa Graduate Theses and Dissertations

This thesis asks, are neighborhood demographic and economic variables connected to COVID-19 infection rates in Tokyo, Japan? I hypothesize that variation in urbanization and neighborhood demographics account for Japan’s low, though not uniform COVID-19 infection rates. This thesis applies several anthropological perspectives: The biocultural perspective because I look at epidemiology of COVID 19 considering socio-cultural, economic, and ecological factors as well as biological susceptibilities. The critical biocultural perspective because I look at how structures of power and inequality may impact health and healthcare access. Biomedical/applied anthropology, well placed to study the current epidemiologic situation of COVID 19 in Japan and …

Antibiotic-Resistant Escherichia Coli And EnterococcusSpp. In Sand And Water At Tampa Bay Beaches, Jennifer K. Sabater

USF Tampa Graduate Theses and Dissertations

As antibiotic resistance in the environment continues to rise there is an increased concern that infections may become harder to treat as bacteria acquire genes for multidrug resistance. Recreational beach waters in the Tampa Bay area are routinely monitored by the Florida Department of Environmental Protection for the presence of fecal indicator bacteria (FIB) such as Escherichia coli and enterococci. Exceedances of beach action values (BAV) 235 CFU/100 mL (E. coli) and 70 CFU/100 mL (enterococci) indicate the presence of fecal contamination which is associated with an increased risk of disease for beachgoers. Antibiotic-resistant E. coli and Enterococcus spp. have …

Chronological Accumulation Of Microplastics In The Gulf Of Mexico And Their Acute Effects On Coral Bleaching, Martina M. Plafcan

USF Tampa Graduate Theses and Dissertations

Microplastics have accumulated in the environment since plastic production began. They have spread to all areas of the globe from marine trenches to mountains and they can be harmful to organisms. However, research on microplastics has only recently begun so it is unclear how they have changed over time in many regions of the ocean and how current oceanic concentrations might affect marine life. Additionally, organisms such as corals are under stress and have been declining due to climate change, so it is not yet known if microplastics exacerbate these threats. My thesis addressed these gaps in the literature by …

Investigating A Potential Sting Modulator, Jaret J. Crews

USF Tampa Graduate Theses and Dissertations

The work contained in this thesis introduces a protein known as STING, an acronym for STimulator of INnterferon Genes, an immunosurveillance ER-membrane bound adapter protein critical to regulating the innate immune response in humans. The first chapter provides an overview of the STING pathway, outlines STING’s potential as a therapeutic target of interest, and delineates the primary challenges researchers encounter when designing small molecules to modulate the activity of STING. Current efforts in developing STING modulators have focused on devising agonists to up-regulate the STING pathway as a possible therapeutic for human cancers based on the chemical scaffold of STING’s …

The Maternal Oral Microbiome Throughout Pregnancy, Rebecca Koerner

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation was to longitudinally examine the salivary microbiome during the second and third trimesters of pregnancy and explore its associations amongst adverse pregnancy outcomes (preterm birth, gestational diabetes mellitus, and hypertensive disorders of pregnancy), perceived stress and depression, and hair/salivary cortisol levels. The specific aims were to (1) explore the literature for studies longitudinally examining the oral microbiome during pregnancy using a scoping review guided by the Arskey and O’Malley framework; (2) to examine the salivary microbiome for changes in diversity and abundance during the second and third trimesters during pregnancy and its association with adverse …

Metabolomic Analysis, Identification And Antimicrobial Assay Of Two Mangrove Endophytes, Stephen Thompson

USF Tampa Graduate Theses and Dissertations

Natural products have played major roles in drug discovery and provided sources for many new drugs. Secondary metabolites, also called natural products, are organic compounds that are produced from fungi, bacteria, and other living organisms. Secondary metabolites produced by endophytic fungi include alkaloids, phenols, peptides, enzymes, polyketides, and terpenes. Endophytic fungi are rich sources of secondary metabolites and demonstrate bioactivity against many human pathogens including the ESKAPE pathogens. This thesis explores the diverse roles of endophytic fungi and their production of unique secondary metabolites for potential drug discovery and development. Secondary metabolites from two endophytic fungi (EG10-21E-2-HDAC & TAP14-34A-2-HDAC), collected …

Synthesis And Antibacterial Testing Of Novel Thiosulfonate Compounds, Lindsay I. Blume

USF Tampa Graduate Theses and Dissertations

Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Staphylococcus aureus (VRSA) are increasing concerns in the medical community. In addition to the threat of Staphylococcus aureus, the increase in number and severity of infections caused by other antibiotic-resistant bacteria is problematic to human health, particularly among the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter). The antibiotics like the penicillins and cephalosporins used commonly to treat MRSA, VRSA, and ESKAPE pathogens have continued to decline in the effectiveness. Our laboratory has developed a number of new families of anti-MRSA and anti-VRSA antibacterial compounds. In this thesis, …

Onsite Water Reuse: Fate Of Indicator Bacteria, Antibiotic Resistant Genes, Environmental Impacts, And User Perceptions, Michelle B. Henderson

USF Tampa Graduate Theses and Dissertations

Changes in water use, climate, and urbanization have shifted how water resources are used and defined. Wastewater is increasingly regarded as a viable resource to address challenges in non-potable and potable water applications. When dealing with water reuse, water quality and quantity are of the utmost importance; and the final product must be suitable for its intended purpose and acceptable to users. The first portion of this research analyzed the performance and environmental impacts of pilot-scale Hybrid Adsorption Biological Treatment Systems (HABiTS) for onsite wastewater treatment and reuse. Various configurations were investigated to increase HABiTS performance and ability to produce …

Bioactivity Of Suberitenones A And B, Jared G. Waters

USF Tampa Graduate Theses and Dissertations

Throughout human history, natural products have formed the foundation of medicine. In ancient times, a myriad of herbs and fungi have been attributed properties of healing and rejuvenation. Today, that foundation still very much exists, as natural product chemists isolate bioactive compounds from plants, fungi, animals, and microorganisms alike. As more of these secondary metabolites are discovered, scientists pursue more and more sources of biodiversity that may yield new and unprecedented compounds. To this end, Antarctica has become of particular note, as its unique environmental conditions and highly isolated nature make for rare and unusual adaptations in its native wildlife. …

Role Of Bmi1 In Acute Lung Injury, María Helena Hernández-Cuervo

USF Tampa Graduate Theses and Dissertations

Acute Lung Injury (ALI) is a set of signs and symptoms that lead to acute hypoxemic respiratory failure characterized by bilateral pulmonary infiltrates not attributed to cardiogenic origin. It is caused by a massive innate immune response, with the migration of white blood cells (neutrophils and macrophages principally) and a cytokine storm, followed by alterations in mitochondrial function, increase in reactive oxygen species production, and oxidative stress that in turn induces more mitochondrial damage. Several studies have shown that mitochondrial alterations are key events in the mechanism of ALI and reducing mitochondrial dysfunction could be a possible target in the …

Determining The Role Of Dendritic Cells During Response To Treatment With Paclitaxel/Anti-Tim-3, Alycia Gardner

USF Tampa Graduate Theses and Dissertations

Intratumoral CD103+ dendritic cells (cDC1) are required for anti-tumor immune responses. In tumors that are poorly responsive to immunotherapeutic approaches targeting T cells, targeting cDC1 represents an alternative approach that may be useful in improving patient response rates. As such, it is critical to understand cDC1 function within tumors, and what may be preventing optimal function of cDC1. TIM-3 is a receptor that is highly expressed by cDC1 in murine and human mammary tumors, and TIM-3 blocking antibodies are currently being evaluated in clinical trials for a number of solid and hematological malignancies. In order to best design combinatorial therapeutic …

Using Artificial Intelligence To Decipher Epigenetic Code Of Drug Resistance In The Deadliest Human Malaria Parasite, Samira Jahangiri

USF Tampa Graduate Theses and Dissertations

Malaria remains one of the immense global public health challenges, with an estimated ~200 million cases worldwide in 2019 despite the remarkable gains in reducing this deadly disease over the past decade. The recent emergence and spread of artemisinin resistance (ART-R) in Plasmodium falciparum will increasingly impede global efforts to control and eliminate malaria. Previous studies have observed broad transcriptional changes and identified several noncoding genetic variants strongly associated with ART-R. The broad transcriptional variations suggest that the malaria parasite uses sophisticated epigenetic regulation to survive under drug pressure. Therefore, evaluating the regulatory effects of noncoding-variants in malaria parasites is …

A Biocultural Analysis Of The Impacts Of Interactions Between West Africans And Europeans During The Trans-Atlantic Trade At Elmina, Ghana, Heidi Ellen Miller

USF Tampa Graduate Theses and Dissertations

This project utilizes a biocultural approach to assess the demographics and health of the West African population from Elmina, Ghana. Elmina, selected by the Portuguese in 1482 as the site of the first European trade fort in sub-Saharan Africa, grew from a small coastal fishing village to a large settlement over the course of more than 400 years of trade and cultural entanglement. Taken over by the Dutch and then ceded to the British, the people of Elmina navigated significant cultural changes, changes and experiences that can be detected in their skeletal remains. Bioarchaeological research concerned with the effects of …

Infectious Disease Modeling And Intervention Planning, Shalome Hanisha Anand Tatapudi

USF Tampa Graduate Theses and Dissertations

Disease outbreaks caused by existing and emerging pathogens pose a serious threat to local and global communities in the form of epidemics and pandemics, respectively. The 2009 H1N1 pandemic and the 2019 COVID-19 pandemic are exemplars of how underprepared both the developed and developing nations are at mitigating pandemics.

Mathematical modeling and simulation of disease outbreaks has served as a powerful tool to understand disease transmission dynamics. They also aid in developing effective intervention strategies. However, existing models are usually particularized to a region, or a specific disease pathogen and interventions used in these models may not translate well to …

Learning Connections Inventory© Validation Using A Physician Assistant Training Program, Roberta Worsham

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to validate the Learning Connections Inventory for use with the military Physician Assistant students at the Interservice Physician Assistant Program at Fort Sam Houston, TX. The 61 participants in this study completed both a demographic survey and the Learning Connections Inventory (LCI©). The results of the demographic questionnaire depicted the characteristics of the participants and the LCI© described the learning patterns of each participant.

Research Questions one, confirmation of the current LCI© model, and two, the instructor assessment and the current model agreement, were answered using confirmatory factor analysis (CFA) and construct validity. The …

Microbial Dark Matter: Culturing The Uncultured In Search Of Novel Chemotaxonomy, Sarah J. Kennedy

USF Tampa Graduate Theses and Dissertations

Although <1% of environmental isolates can be cultured, insights into the culturable fraction refine future techniques to unlock the cryptic biodiversity. This cryptic biodiversity is omnipresent within culture-independent surveys of environmental bacterial populations. Subsequently, these elusive organisms are termed “microbial dark matter” due to the intrigue surrounding their potential taxonomic and biosynthetic impacts. To this end of investigating bacterial-specific dark matter, we employed high-throughput culturing techniques to examine Gulf of Mexico sediments and sponges for chemotaxonomically important bacteria. Through stepwise implementation of permissive and selective recovery techniques, we succeeded in recovering over 150 unique bacterial isolates. We observed several isolates that pose as chemotaxonomically interesting, including several strains that belong to critical bioremediation taxa. In addition to these biotechnological-amenable isolates, we have also succeeded in culturing taxa historically attributed with antibacterial discoveries. One such isolate was a previously sponge-associated organism belonging to the rarely cultured Verrucosispora genus. Through extensive comparative genomics, we identified it as a novel species and subsequently named it Verrucosispora sioxanthis sp. nov. with the published genome serving as a resource for continued microbial dark matter investigations. Following continued passaging of the organism, we observed improved growth, biomass, and liquid growth dispersal within each increasingly sub-passaged generation of the isolate. As such, the laboratory attenuation, or domestication, of the organism warranted in-depth whole genomic analyses to generate insight into the increased laboratory sustainability. Through phenotypically-guided bioinformatics, we determined that the domestication was mediated through genome-wide selective pseudo- or non-pseudogenization of genes. As the previously sponge-associated organism evolved with the selection pressures of the static laboratory, we demonstrate that isolate effectively underwent genomic atrophy to resolve into a largely stable, sustainable laboratory-domesticated strain. Collectively, our chemotaxonomic discovery pipeline expands on the culturable bacterial biodiversity and ultimately provides a previously uncultured model organism for initial forays into resolving the microbial dark matter knowledge gap.

Investigation Of An Alternative Protocol For The Production Of Sars-Cov-2 Antigenic Proteins, Nichole Ninaltowski

USF Tampa Graduate Theses and Dissertations

With the COVID-19 pandemic showing no signs of slowing down, large-scale antigenic protein production is still needed for surveillance using serologic assays. From screening to vaccines to biotherapeutics, being able to produce the proteins for these assays is essential; however, the current gold standard method for producing SARS-CoV-2 spike proteins is prohibitively expensive for most research groups.

Alternative methods of transfecting mammalian cells to produce recombinant proteins that are relatively inexpensive have been used for years. Unlike the expensive, commercially available lipid-based methods, other established methods such as polyethyleneimine (PEI), are considerably easier, and cheaper to meet the needs of …

Seasonality In Competence To Transmit West Nile Virus For A Widespread Reservoir, Kyle L. Koller

USF Tampa Graduate Theses and Dissertations

All organisms face a central conflict; a limited supply of resources must beoptimally allocated to competing biological functions. Utilizing robust immune defenses can be energetically costly;, thus, immune function is often diminished when other processes, such as reproduction, molt, or migration demand energy. As wild animals residing in temporally dynamic environments face seasonal patterns in resource availability, infectious disease risk, and environmental suitability, many species have evolved endogenous biological rhythms so that the performance of reproduction, molt, or migration and the associated trade-off with immune function be completed when doing so best promotes fitness. In turn, this demand to optimize …

Synthesis Of A Multimodal Ecological Model For Scalable, High-Resolution Arboviral Risk Prediction In Florida, Sean P. Beeman

USF Tampa Graduate Theses and Dissertations

West Nile virus (WNV) and Eastern Equine Encephalitis virus (EEEV) represent the two greatest endemic arboviral risks to the state of Florida. Currently, no approved human vaccine exists for the prevention of either virus. In the absence of a vaccine, effective disease surveillance is paramount for public health. In Florida, WNV and EEEV sentinel chicken surveillance is conducted by mosquito control programs operated at the county, municipality, or special taxing district level. This program was implemented in 1978 following human outbreaks of St. Louis Encephalitis virus (SLEV) that occurred between 1959 and 1977, with initial sentinel coops placed in proximity …