Digital Commons Network^™

Synthesis Of Small Molecules For The Treatment Of Infectious Diseases, Elena Bray

USF Tampa Graduate Theses and Dissertations

Drug discovery’s impact on modern healthcare cannot be measured. In the past year, the world has watched scientists all around the globe work together to help protect the public from the novel coronavirus pandemic. Although sometimes slow and costly, drug discovery is important for advancing modern society. Drug discovery is at the core of treating diseases and in particular, infectious diseases. In the first chapter an overview of drug discovery will be discussed in a brief manner.

The second chapter will introduce infectious diseases. In modern society, the development of small molecule treatments for treating bacteria, viruses, and fungi has …

Targeting Modern Pathogens With Rational Drug Design, Michael Dominic Sacco

USF Tampa Graduate Theses and Dissertations

Since the first living organisms appeared on earth, their very existence was dependent on their ability to contend for resources. To this day, organisms are perpetually competing for resources, and in doing so, have evolved highly effective defense mechanisms and systems for propagation. Although invisible to the eye, we live in a world teeming with microbes and viruses. While many are harmless, others cause widespread disease. One of the key niches for these pathogens, particularly bacteria, are health-care facilities. These health-care facilities have a high concentration of immunocompromised patients and rely heavily on antibiotics, which strongly contributes to antibiotic resistance. …

Metal Oxide Sensor Array Test Bed Prototype For Diagnostic Breath Analysis, Tiffany C. Miller

USF Tampa Graduate Theses and Dissertations

Detection of specific target biomarkers is a critical step for electronic nose technologies in breath analysis. Electronic nose technologies have been successfully demonstrated in the air quality, meat processing, and the citrus industries, as well as cancer clinical trials. The novel design, assembly, and implementation of a test bed prototype of an electronic nose system is introduced in this Dissertation. To verify the feasibility of a test bed prototype design, a Corona Virus Disease 2019 (COVID-19) breath simulation mixture of alcohol, acetone, and carbon monoxide was presented to the test bed prototype in the laboratory. The COVID-19 breath simulation mixture …

Diaspora By Design: The Systematic Displacement Of Tampa's African-American Populace, 1925-1974, Shannon D. Bruffett

USF Tampa Graduate Theses and Dissertations

Diaspora by Design: The Systematic Displacement of Tampa’s African-American Populace, 1925-1974 is a political and economic history that maps the transformation of the city’s urban landscape through a variety of federal programs. It examines the the ways that Tampa city leaders employed a variety of federal programs, and funding, to shape the economic, social, and cultural environment of Tampa’s Black population over the course of five decades.

The Effects Of Agrochemical Pesticide Applications On Target And Off-Target Species In Aquatic Ecosystems, Bryan K. Delius

USF Tampa Graduate Theses and Dissertations

The increase in the global human population is driving agricultural expansion, which increases the likelihood and degree by which agrochemicals impact aquatic systems. It is projected that by 2100 the global human population will exceed 11 billion, and to feed the increased human population, the necessary increase in agricultural infrastructure is estimated to double irrigation, nearly triple fertilizer applications, and increase pesticide applications by tenfold. It stands to reason that wetlands impacts from agriculture are also likely to increase as the industry expands its operation to meet the globally growing demand for food.

Wetlands are highly productive habitats that not …

Successful Aging In Adults Who Stutter: Exploring Predictors Of Physical And Mental Health-Related Quality Of Life, Amanda Kelly

USF Tampa Graduate Theses and Dissertations

This study was conducted to investigate predictors of physical and mental health quality of life in aging adults who stutter (AWS). Our goal was to identify factors contributing to successful aging in this talker group. The aging population is expected to increase exponentially over the next twenty years. Factors influencing self-perceived health-related quality of life have yet to be investigated in aging AWS.

An online survey was completed by a total of 40 AWS, age 50 years and older, recruited from the Greater Tampa Bay region and nationally. The survey comprised multiple measures that probed for information regarding self-perceived health …

Flow Visualization And Aerosol Characterization Of Respiratory Jets Exhaled From A Mannequin Simulator, Sindhu Reddy Mutra

USF Tampa Graduate Theses and Dissertations

It is important to understand the airborne transmission of infectious diseases due to the COVID-19 pandemic. Physical experiments were conducted to understand the dispersion of aerosols emitted from a mannequin simulator replicating human tidal breathing through the nose and mouth with and without a protective face mask in an enclosed space. Flow patterns are observed via high-speed visualization, and the concentration and size distribution of the particles is measured as a function of distance from the mannequin using an optical particle sizer. Masks were shown to effectively reduce the horizontal dispersion of aerosol for both mouth and nose breathing. Further, …

The Extended Parallel Processing Model (Eppm) And Risk Perceptions Of Twitter Messages Related To Covid-19, Muhammad E. Rasul

USF Tampa Graduate Theses and Dissertations

Since its genesis, the novel coronavirus or COVID-19 has claimed millions of lives across the world and has infected many more. The population in the US experienced one of the worst outbreaks of COVID-19 in the world, with the number of deaths rising monolithically at one point. Although the development and inoculation of a large number of the public has helped, newer variants threaten to revitalize the growth and spread of COVID-19. Scholars have extensively studied health messages related to COVID-19 in a variety of contexts. However, little attention has been paid to the risk perceptions of health messages related …

Transparent Planar Micro-Electrode Array For In-Vitro Electric Field Mediated Gene Delivery, Raj Himatlal Shah

USF Tampa Graduate Theses and Dissertations

Gene delivery is the process of delivering the modified gene into the human cell. There are two types of gene delivery depending on where the target cells are. The in-vivo gene delivery deals with delivering the altered genes into the cells while still in the host body. While another approach is to extract the cells from the host body, transfer the modified gene into cells and then put these cells back into the host body, this approach is called the ex-vivo method.

A vector is a carrier that carries the modified gene to the host cell or body. Hence, depending …

A New Age Of Telehealth: Pediatric Speech-Language Pathology Services During The Covid-19 Pandemic And Beyond, Deborah R. Campbell

USF Tampa Graduate Theses and Dissertations

The COVID-19 pandemic potentially changed the landscape of how speech-language pathologists provide services. Prior to March 2020, pediatric speech-language therapy provided via telehealth was limited; however, the COVID-19 pandemic caused a worldwide conversion from in-person care to a remote service delivery model. This conversion brought to the fore possible benefits and utility of telehealth use. Yet, clinicians potentially experienced barriers to its use, including the lack of validity and reliability evidence for remote administration of several pediatric assessments. For the viability of remotely delivered speech-language services to continue and evolve in a post-pandemic world, further research is needed to identify …

Health Risk Assessment Of Local Populations Ingesting Water With Naturally Occurring Arsenic And Fecal Related Contaminants In Lake Atitlan, Guatemala, Marisol Alvarez

USF Tampa Graduate Theses and Dissertations

Lake Atitlan, Guatemala acts as a sink for wastewater discharge and as a source of drinking water. The populations living in the lake basin are primarily indigenous with a large percentage (70%) living in poverty. Wastewater effluent entering the lake poses an immediate health risk to those using it as a drinking-water source. Additionally, the lake is surrounded by three volcanoes and the water has concentrations of naturally occurring arsenic above the recommended reference level of 10 µg/L. Arsenic is acutely poisonous at high concentrations and has carcinogenic and neurological effects when chronically exposed at low concentrations.

The goal of …

An Examination Of Covid-19 Health Behaviors And Public Health Messaging Using The Health Belief Model And Organization-Public Relationship Quality, Aaron L. Nichols

USF Tampa Graduate Theses and Dissertations

In the context of the most severe pandemic in over 100 years, this study examined public health behavior and public health messaging using the health belief model (HBM) and organization-public relationships (OPR) as frameworks. The study employed a cross-sectional survey of students (N = 288) and employees (N = 203) at a large public university in the southeastern United States. First, the study empirically tested the components of the HBM as determinants of engaging in public health behaviors meant to slow or prevent the spread of COVID-19 and found all components of the model to be significantly related to engaging …

Development And Validation Of An Isothermal Amplification Assay For Eastern Equine Encephalitis Virus, Mikayla D. Maddison

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus (EEEV) is a neurotrophic alphavirus for which there is no effective treatment or vaccine for humans. Periodic outbreaks in the Eastern United States represent an ongoing public health problem; Florida serves as the reservoir for EEEV for the rest of the country. Reverse transcription-polymerase chain reaction (RT-PCR) is the current gold standard for molecular diagnostic testing of the presence of EEEV in vectors. However, RT-PCR is technically complex and can be difficult for mosquito control districts to utilize. In order to provide a simple, cost-effective alternative for mosquito surveillance and control, a novel one step reverse …

Mealtimes In Early Childhood Education Centers During Covid-19: A Mixed-Methods Assessment Of Responsibilities, Interactions, And Best Practices, Joanna Mackie

USF Tampa Graduate Theses and Dissertations

Background: Childhood obesity is a public health problem associated with many co-morbidities. The majority of young children in the United States (U.S.) attend formal early childhood education (ECE) programs, often consuming the majority of daily calories and engaging with teachers during mealtimes. Mealtime best practices support children’s development of healthy eating habits. This dissertation aimed to understand how COVID-19 has influenced mealtimes in ECE centers, including (1) the division of responsibility between adults and children during mealtimes, (2) the child feeding dynamic, and (3) how mealtime best practices have been included during COVID-19.

Methods: This dissertation used a concurrent mixed-methods …

Listening To Women: Using A Mixed-Methods Approach To Understanding Women’S Desires And Experience During Childbirth, Nicole Loraine Falk Smith

USF Tampa Graduate Theses and Dissertations

This dissertation is based on evolutionary medicine and applied anthropological research where the narratives and biology of modern women are used to inform evolutionary theory, specifically obligate midwifery, and modern birth practices. Improvements to the US maternity care systems are needed as rates of maternal mortality and morbidity are continually higher than rates in other technologically advanced nations. Additionally, women birthing under the technocratic model of care, as is predominant in the US, report trauma and post-traumatic stress disorders due to their birthing experience, and more women are experiencing postpartum depression. Thus, I seek to use an evolutionary medicine perspective …

Epigenetic Potential In An Introduced Passerine, Haley E. Hanson

USF Tampa Graduate Theses and Dissertations

Epigenetic modifications play a critical role in numerous processes throughout the lifetime of an organism by influencing gene regulation. Responsive to both endogenous cues and external stimuli, epigenetic modifications are key mechanisms underlying phenotypic plasticity. Epigenetic potential, or the capacity for phenotypic plasticity mediated by epigenetic modifications, can be encoded within the genome via genetic variation underlying aspects of epigenetic modifications. For example, one type of epigenetic modification, DNA methylation, predominately occurs at CpG motifs in vertebrates. The number of CpG sites within the genome then represents the capacity for DNA methylation to occur and is one form of epigenetic …

Mechanisms And Mitigation: Effects Of Light Pollution On West Nile Virus Dynamics, Meredith E. Kernbach

USF Tampa Graduate Theses and Dissertations

Light pollution, or the presence of unnatural light at night, is a pervasive and growing problem across the globe. While often pictured in urban centers, light pollution is far reaching and can affect seemingly safe and minimally developed environments. For example, agricultural communities with artificial lighting near facilities can generate such light pollution in rural areas. Further, streetlights and illuminated billboards along roads and highways can generate light pollution far from cities. Given how pervasive this anthropogenic stressor is, it is surprising that not much is known about how artificial light at night, or ALAN, affects humans or wildlife, especially …

Screening Of Pregnant Women With Opioid Use Disorder: Identifying Factors Impacting Implementation Of Screening Recommendations Using The Theoretical Domains Framework, Tara R. Foti

USF Tampa Graduate Theses and Dissertations

Background: Prenatal opioid exposure is a growing problem in the United States with high and increasing rates of opioid use and opioid use disorder during pregnancy. Almost 23% of pregnant women enrolled in Medicaid programs filled an opioid prescription during pregnancy in 2007, marking a 21.1% increase since 2000. Maternal opioid use during pregnancy is associated with a variety of poor maternal, pregnancy and infant outcomes, including overdose, pregnancy-associated death, prematurity, low birth weight and Neonatal Opioid Withdrawal Syndrome. To optimize prenatal care and pregnancy-associated outcomes, the Alliance for Innovation in Maternal Health, in collaboration with The American College of …

Self-Protection In Cyberspace: Assessing The Processual Relationship Between Thoughtfully Reflective Decision Making, Protection Motivation Theory, Cyber Hygiene, And Victimization, C. Jordan Howell

USF Tampa Graduate Theses and Dissertations

The current study, using structural equation modeling, assesses the processual relationship between thoughtfully reflective decision making (TRDM), theoretical constructs derived from protection motivation theory (PMT), cyber hygiene, and online victimization to determine the cognitive decision-making process that leads to the adoption of online self-protective behaviors, which reduces the occurrence of victimization experiences. Findings, derived from a general sample of Internet users in the United States, reveal: (1) engagement in cyber hygiene practices, as a form of target hardening, decreases Internet users’ experiences with online victimization; (2) thoughtfully reflective decision makers, in the face of cyber threats, develop higher threat appraisals …

An Evaluation Of Video Feedback With And Without Video Modeling To Enhance Barbell Squat Form, Alexandra Olles

USF Tampa Graduate Theses and Dissertations

Previous studies have assessed the use of video interventions to enhance athletic performance. However, few studies have evaluated the use of video interventions to improve form in weightlifting. The present study evaluated the effects of video feedback (VF) with and without video modeling (VM) to enhance barbell squat form with novice and typically developing adults. The results showed that VF increased performance to near 100% for one participant. For the other VF increased performance to moderate levels and the addition of VF only slightly increased performance

Evaluating The Development And Implementation Of Campus-Based Sexual And Interpersonal Violence Prevention Programming, Robyn Manning-Samuels

USF Tampa Graduate Theses and Dissertations

1 in 5 women in institutions of higher education experience an attempted or completed sexual assault every year. Sexual and interpersonal violence prevention programming is one of the main ways colleges and universities attempt to address this issue. The purpose of this exploratory qualitative pilot study is to evaluate the development and implementation of campus-based sexual and interpersonal violence prevention programming, looking specifically at facilitators and barriers for prevention staff, what they experience as preventionists, and how COVID-19 has impacted these essential programs. Ten preventionists were recruited from the Campus Advocacy and Prevention Professionals Association for 90-minute interviews. Results of …

Defining Codes Based On The Consolidated Framework For Implementation Research In The Context Of The Implementing Universal Lynch Syndrome Screening, Jasmine A. Burton-Akright

USF Tampa Graduate Theses and Dissertations

Lynch syndrome (LS) is the most common hereditary cause of both colorectal cancer (CRC) and endometrial cancer (EC). Universal tumor screening (UTS) of newly diagnosed EC and CRC patients has been shown to be both an effective and economical approach to identify patients with LS and subsequently reduce future cancer s for patients and their family members. Despite its efficacy, LS UTS has not been consistently adopted across hospital systems and existing programs vary widely in their structure and execution. The Implementing Universal Lynch Syndrome Screening (IMPULSS) study aims to determine the critical factors necessary for successful implementation of LS …

Comparison Of The Effectiveness Of Disinfectant-Impregnated Wipes Versus Detergent Wipes For Surface Decontamination, Jacob Amadin

USF Tampa Graduate Theses and Dissertations

Over time, there is an increased need to adopt more advanced and effective ways of disinfection. The emergence of the coronavirus-2019 (COVID-19) pandemic reminded the world on the importance of disinfection. Disinfection has been a routine in the healthcare setting. However, with the current pandemic problem, it is more prominently and persistently done in the domestic setting and other business settings beyond healthcare. As such, the use of most disinfectants and detergent wipes to achieve a near sanitized environment has become a common practice around the globe. This research presents the efficacy of disinfectant impregnated versus detergents wipes through comparison. …

Promoting Hpv Vaccination With Vaccine-Hesitant Parents Using Social Media: A Formative Research Mixed-Method Study, Silvia Sommariva

USF Tampa Graduate Theses and Dissertations

Vaccine hesitancy, the refusal or delay in complying with set immunization schedules, has been proclaimed one of the “ten threats to global health” by the World Health Organization in 2019. Since then, the COVID-19 pandemic has reinforced the importance of vaccine promotion and management of vaccine hesitancy. While anti-vaccine arguments have not surprisingly evolved much over the past decades, the speed at which those ideas spread has. Web-based technologies, such as social network sites (SNSs), provide a fertile ground for vaccine-related misinformation to spread. Therefore, SNSs are a primary channel to scale efforts to address vaccine hesitancy, using customized approaches. …

Tourism, Education, And Identity Making: Agency And Representation Of Indigenous Communities In Public Sites Within Florida., Timothy R. Lomberk Ii

USF Tampa Graduate Theses and Dissertations

National parks have a history of complex relations with Native communities beginning with the advent of the National Park Service to representation of Native histories in the present. I will focus on two specific cases located within Florida, utilizing the lens of authorized heritage to show challenges of representation; and the range of ways that representation has been addressed and are currently being addressed with respect to Native communities in Florida. I utilize ethnohistorical and ethnographic methods to explore issues of representation in public spaces; such as museums and national parks. I am interested in critical representations of Native American …

Analysis Of The Self-Directed Learning Readiness Of New Graduate Registered Nurses, Denise Hain

USF Tampa Graduate Theses and Dissertations

Self-directed learning readiness is the level of ability and willingness to manage one’s own learning. Research has been conducted on the self-directed learning readiness of both student nurses and professional nurses. However, research has not expanded to focus on the self-directed learning readiness of new graduate Registered Nurses entering the workforce. The purpose of this study was to identify the self-directed learning readiness of new graduate Registered Nurses entering the workforce in a hospital setting. The study examined their self-directed learning readiness profile; the differences in scores based on nursing degree, nursing program, and age; and the differences in scores …

Spatial And Temporal Determinants Associated With Eastern Equine Encephalitis Virus Activity In Florida, Kristi M. Miley

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus (EEEV) is considered the most pathogenic mosquito-borne illness in the United States. Human mortality has been estimated to range between 35 to 75%, with horses experiencing mortality rates greater than 90%. A large number of EEEV cases occur in Florida. Though we have come a long way since the first human infection was identified in the 1930s, there is still much to learn regarding the virus’s ability to maintain transmission year-round in Florida. Phylogenic studies support that Florida may serve as the geographic reservoir for EEEV. This research investigated spatiotemporal and ecological variables associated with risk …

A Novel Magnetic Respiratory Sensor For Human Healthcare, Kee Young Hwang

USF Tampa Graduate Theses and Dissertations

Breathing is vital to life. Therefore, the real-time monitoring of a patient’s breathing pattern is crucial to respiratory rehabilitation therapies such as magnetic resonance exams for respiratory-triggered imaging, chronic pulmonary disease treatment, and synchronized functional electrical stimulation. While numerous respiratory devices have been developed, they are often in direct contact with a patient, which can yield inaccurate or limited data. In this study, we developed a novel, non-invasive, and contactless magnetic sensing platform that can precisely monitor a patient’s breathing, movement, or sleep patterns, thus providing efficient monitoring at a clinic or home. A magneto-LC resonance (MLCR) sensor converts the …

The Impact Of Myeloid-Mediated Co-Stimulation And Immunosuppression On The Anti-Tumor Efficacy Of Adoptive T Cell Therapy, Pasquale Patrick Innamarato

USF Tampa Graduate Theses and Dissertations

Adoptive T cell therapy (ACT) in combination with lymphodepleting chemotherapy is an effective strategy to induce the eradication of tumors, providing long-term regression in cancer patients. However, only a minority of patients that receive ACT with tumor infiltrating lymphocytes (TILs) exhibit durable benefit. Thus, there is an urgent need to define strategies that potentiate anti-tumor activity conducted by adoptively transferred T cells. In these studies, we aimed to identify novel strategies to enhance the therapeutic efficacy of ACT. Accordingly, we describe the disparate roles of myeloid cells in the context of ACT characterized by the augmentation of TIL proliferation in …

Soil Characteristics Associated With Horse Cases Of Eastern Equine Encephalitis Virus In Florida, Fulya Guzelkucuk

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus (EEEV) is a mosquito-borne virus found in Florida that affects humans and horses. Also, EEEV is an agricultural land management issue in Florida, as it causes mortality in large numbers of horses each year and sometimes humans. This study investigates the characteristics of soils associated with horse cases of EEEV in the State of Florida, focusing on differences in soil characteristics between summer and winter cases. This study analyzed a total of 676 EEEV cases during 2005-2018, including 611 summer cases and 65 winter cases. Soil characteristics that were examined include: soil texture, drainage class, hydrology …