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Group B Streptococcus Intestinal Barrier Disruption And The Protective Role Of Butyrate, Kristen Dominguez

USF Tampa Graduate Theses and Dissertations

Group B Streptococcus (Streptococcus agalactiae; GBS) is a leading cause of neonatal sepsis worldwide. Intrapartum antibiotics in GBS-colonized mothers significantly reduced the incidence of early-onset sepsis occurring in the first week of life; however, there are currently no prophylactic interventions for late-onset sepsis, occurring within the first 3 months of life. GBS is a pathobiont that primarily colonizes the gastrointestinal tract and can cross the intestinal barrier via both the paracellular and transcellular routes, causing invasive disease. Neonatal susceptibility to GBS intestinal translocation is mediated by inadequate epithelial barrier function and microbiome immaturity; however, the mechanisms by which GBS exploits …

“Siguiendo Adelante”/ "Moving Forward:" Gender-Based Violence (Gbv) Service-Seeking And Survivorship Among Indigenous Guatemalan Women, Caitlynn C. Carr

USF Tampa Graduate Theses and Dissertations

This dissertation examines Indigenous women’s access to and beliefs regarding gender-based violence (GBV) services and the Guatemalan legal system. It illuminates the legal, structural, and institutional challenges and power dynamics associated with seeking support and justice for women in Guatemala, as well as why and how women decide to seek formal assistance. This research also investigates changes in attitudes and beliefs regarding women’s rights, gender roles, and formal and informal forms of support for GBV survivors. Utilizing a mixed-methods, engaged anthropological approach during 10 months of ethnographic fieldwork in two primary locations in Guatemala, this dissertation explicates qualitative data collected …

Macrophage-Driven Car T Cell Resistance In B Cell Lymphoma, Sae Bom Lee

USF Tampa Graduate Theses and Dissertations

Chimeric antigen receptor (CAR) T-cell therapy has revolutionized the treatment of patients with relapsed/ refractory large B cell lymphoma (LBCL). Despite its promising efficacy, a subset of patients experiences primary resistance or relapse. In Chapter 2, we explore the tumor microenvironment (TME)-induced resistance mechanisms in patients with LBCL. Analysis of the intratumoral immune infiltrates in pre-infusion tumor biopsies revealed that the levels of immunoregulatory macrophages were elevated in patients who responded poorly to axicabtagene ciloleucel (axi-cel). Using preclinical mouse B cell tumor models, we found that the CAR T-cell-produced interferon-gamma (IFN-γ) enhanced the expression of inducible nitric oxide synthase (iNOS, …

Essays On The Determinants Of Women's And Children's Health In Bangladesh, Md Shahjahan

USF Tampa Graduate Theses and Dissertations

My dissertation is titled "The Determinants of Women's and Children's Health in Bangladesh." The first chapter studies the impact of a Bangladeshi schooling program on child immunization rates. The program provided a stipend and other incentives to attend secondary school, significantly increasing rural girls' education. I hypothesize that this would lead to higher immunization rates in the next generation since better-educated mothers have better knowledge of health practices. The study uses a regression model ("difference-in-differences"), which is based on differential exposure to the 1994 Female Secondary School Stipend Program (FSSSP) by birth cohort and rural residence. I find that the …

Development Of Novel Analytical Methods For The Investigation Of Mammalian Tissues And Cells, Grace A. Binder

USF Tampa Graduate Theses and Dissertations

This dissertation endeavors to advance the frontiers of analytical chemistry by addressing critical challenges across three distinct chapters. In the first chapter, the focus is on the evaluation of digestion methods for the analysis of trace metals in mammalian tissues and the Standard Reference Material NIST 1577c. The primary goal is to optimize methodologies, laying the groundwork for reliable trace metal analysis and furthering our understanding of their roles in biological systems.

Transitioning into the realm of immunomodulation, the second chapter elucidates immune response, disease and treatment through the optimization and discovery of STING modulators, utilizing the luciferase assay for …

Exploring The Associations Between Drug Resistant Mutations, Low-Level Viremia, And Virological Failure In Persons With Hiv In Florida, Adriana Campos

USF Tampa Graduate Theses and Dissertations

Virologic suppression is an essential part to a successful regimen for Persons with HIV (PWH) and the 95-95-95 initiative. Yet, PWH experiencing episodes of low-level viremia (LLV) may be more prone to experience virological failure (VF). Currently, studies debate the clinical relevance of LLV and its effect on virological outcomes, but none have explored its interconnectedness with drug resistant mutations (DRM) and antiretroviral treatment (ART) adherence – all integral elements in predicting virological outcomes.

The goal of the proposed study was to contribute to the knowledge gap regarding the interconnectivity of drug resistant mutations, low-level viremia, adherence, and virological failure …

Community-Centered Early Warning For Outbreaks In Humanitarian Emergencies: Evaluating Community-Based Surveillance For Priority Communicable Diseases Among Internally Displaced Populations In Iraq, Caitlin M. Wolfe

USF Tampa Graduate Theses and Dissertations

Background: Community based surveillance (CBS) is the organized and rapid collection of information from health events identified in the community and includes the systematic detection and reporting of events of public health significance. While used globally to enhance the detection of priority diseases, CBS has not been routinely deployed in Iraq, where a once-robust health system has been devastated by decades of war, international sanctions, and large-scale population displacement. During infectious disease outbreaks, it is imperative to quickly detect and isolate suspect cases, investigate confirmed cases, and trace and monitor the contacts of confirmed cases. The COVID-19 pandemic underscored the …

Risk Assessment Of Blood-Borne Pathogen Exposure For Emergency Responders, Jill A. Ireland

USF Tampa Graduate Theses and Dissertations

Emergency responders face a significant risk of exposure to blood-borne pathogens (BBPs), such as HIV and hepatitis, due to the nature of their work environment. This study investigates this issue utilizing a dual approach of data analysis and online surveys to assess exposure rates, risk perceptions, factors that contribute to risk and prevention practices. In addressing issues ranging from personal protective equipment (PPE) usage to levels of concern surrounding blood and bodily fluid contact, the findings underscore the need for tailored safety measures, comprehensive education, and improved prevention strategies.

The examination of 346 worker's compensation exposure claims highlighted a significant …

Health Status In An Urban-Dwelling, Subtropical Frog Species (Lithobates Sphenocephalus) And Its Relation To Ranavirus And Hemoparasites, Gabrielle M. Wickersham

USF Tampa Graduate Theses and Dissertations

Urbanization poses significant challenges to wildlife, including amphibians. The present study uses a multi-parameter approach that incorporates veterinary techniques to investigate the current health status of the adult southern leopard frogs (Lithobates sphenocephalus) in Tampa Bay, Florida, amidst urban stressors including nutrient loading, fragmented habitats, and light and noise pollution. Novel values for fluctuating asymmetry (e.g., variation between left and right bilateral traits; FA), body condition (CF), stress (e.g., neutrophil:lymphocyte (N:L) ratio), and inflammation (e.g., neutrophil morphologic abnormalities, parasite load) are reported. I also investigate the relationship of overall health with ranavirus presence and hemoparasite infections. Ranavirus was absent in …

Preliminary Investigation Of Differences In Host Mitochondria Membrane Potential During Toxoplasma Gondii Infection, Bryan L. Ortega

USF Tampa Graduate Theses and Dissertations

Toxoplasma gondii is an obligate intracellular protozoan with a broad host range of hosts, including various warm-blooded mammals, including humans. While infections can often be asymptomatic in immunocompetent individuals, they can pose significant risks to those who are immunocompromised, as well as pregnant women. Toxoplasmosis represents a significant burden on public health in the United States, with over 800,000 annual infections reported, often leading to severe complications such as symptomatic eye disease and congenital infections. Recent attention has developed regarding its potential association with human neuropathies such as Alzheimer's disease, dementia, and mood disorders. This project investigated differences in host …

The Effect Of The Covid-19 Pandemic On The Environmental Perceptions Of College Students, Sydney H. Kaylor

USF Tampa Graduate Theses and Dissertations

In this study, the environmental perception changes in college students during the COVID-19 pandemic were investigated. During the almost worldwide lockdown between March and December 2020, many students experienced dramatic changes in their socioeconomic life that have affected their well-being, ability to learn, and environmental perceptions. Students were sent home from college campuses, many businesses were shut down, and changes were seen in the environment throughout the world. The many industries and businesses that were shut down decreased the pollution that was in the atmosphere, in our water, and on the land. In many places, lockdown policies lead to improved …

Role Of The Glutamine-Polyamine-Hypusine Axis In Cd8+ T Cell Fate And Function, Aya Gamal Aly Sadek Elmarsafawi

USF Tampa Graduate Theses and Dissertations

T cells are critical effectors of the immune response against pathogens and malignancies and, given the recent success of T-cell immunotherapies in cancer, there is now an urgent need to enhance the efficacy and durability of T-cell immune responses to improve anti-tumor immunity. Formation of memory T cells is a critical step of a successful immune response as it ensures maintenance of long-term immunity and protection from re-exposure to foreign antigens. Notably, metabolism regulates the fate and function of T cells and is a powerful tool to modulate T-cell activity and memory differentiation. Accordingly, metabolic interventions to augment T-cell memory …

Online News Portrayal During The Covid-19 Health Crisis And Journalism's Role In Misinformation In The U.S., Michou Ducilon

USF Tampa Graduate Theses and Dissertations

The COVID-19 pandemic has brought a wealth of new information regarding the virus's origin and treatment. Regrettably, the vast amount of online information has resulted in the proliferation of misinformation, as the World Health Organization reported. To investigate whether journalists may have contributed to this problem, a qualitative analysis was conducted on 15 news articles from top-tier media outlets. Using NVivo software, the articles were analyzed to identify linguistic words and visuals that fell into coding categories based on Behrendt's (2008) contextual analysis for evaluating text. Each article was then coded again to identify common themes. During this process, new …

Chemical Analysis Of Metabolites From Mangrove Endophytic Fungus, Sefat E Munjerin

USF Tampa Graduate Theses and Dissertations

Natural products hold a significant place in drug discovery for their abundant and unique secondary metabolites. These secondary metabolites which encompass a range of organic compounds such as alkaloids, phenols, peptides, flavonoids, polyketides, and terpenoids, can easily be exploited for drug development. Endophytic fungi, residing harmoniously with its host, have emerged as generous producers of bioactive secondary metabolites, displaying efficacy against a wide array of human pathogens, including the challenging ESKAPE pathogens.

The research explores endophytic fungi and their prospective for drug discovery and development through the synthesis of distinctive secondary metabolites. Endophytic fungus, HM13-26C-2B, collected from Honeymoon Island, Florida …

Developing A Point-Of-Care Detection Kit For Parkinson’S Disease Using Gold Nanoparticle-Based Lateral Flow Assay, Ali Aljassabi

USF Tampa Graduate Theses and Dissertations

Parkinson’s disease (PD) is the second most common neurodegenerative disease after Alzheimer’s disease and the fastest-growing neurodegenerative disorder in the world. The major issue with PD is that it takes several years of biological changes until the patient starts to experience physiological symptoms that could affect their daily lives. The major hallmark of PD is the appearance of Lewy body inclusions in the neuron’s cytoplasm, which is rich in a protein called α-Synuclein (α-Syn). Several studies have shown that the levels of α-Syn in blood increase because the excess α-Syn is released from neurons into the bloodstream through extracellular vesicles. …

Clinically Applied Anthropology: A Syndemic Intervention., Jason W. Wilson

USF Tampa Graduate Theses and Dissertations

This dissertation demonstrates that a critical, clinically applied anthropology is possible by testing a hypothesis that a syndemic intervention, and use of a structural vulnerability assessment tool, can achieve improved healthcare outcomes. A critical, clinically applied anthropology integrates social scientists into healthcare delivery, alongside biomedical providers, through the co-creation of new diagnostic and patient care pathways that utilize anthropological methods (ethnographically informed care, syndemics, thematic/mixed methods data analysis) and advance anthropological theory (biomedicine and culture, structural violence, structural competency/vulnerability, ontology, assemblage theory, and entanglements) to decrease healthcare inequities.

Medical anthropology has previously engaged biomedicine and other attempts at a clinical …

Development Of Antiviral Peptidomimetics, Songyi Xue

USF Tampa Graduate Theses and Dissertations

Protein-protein interactions (PPIs) are essential for biological processes and are associated with a number of diseases, including cancer, infectious diseases, and neurodegenerative diseases. As a result, modulation of PPIs has been recognized as one of the most promising strategies to develop the novel drugs. Peptide modulators always exhibit higher specificity and affinities with targets than small compounds or monoclonal antibodies, but their broad medicinal effectiveness is constrained by their poor bioavailability and biostability. Peptidomimetics, which have been developed to mimic the structure as well as function of bioactive peptides and proteins, have shown excellent potential in protein surface mimicry and …

Role Of Short Interspersed Nuclear Elements (Sines) Of Maternally Imprinted Microrna Clusters In Hemochorial Placental Antiviral Immunity, Ishani Wickramage

USF Tampa Graduate Theses and Dissertations

The evolution of hemochorial placentas in which the fetal trophoblast is bathed in maternal blood has vastly improved fetal development and survival in primates and rodents, due to the enhanced efficiency of nutrient, gas and waste exchange between the mother and the fetus. However, the extensive contact between the fetal tissues and maternal blood in these placentas also poses an increased risk of vertical transmission of pathogens such as viruses from the mother to the fetus. In response to this selection pressure, hemochorial placentas have evolved robust defense mechanisms to protect the immunologically naive fetus. Unlike somatic cells that require …

Synthesis Of Tools For The Investigation And Treatment Of Infectious Diseases, Grant Lawrence

USF Tampa Graduate Theses and Dissertations

Modern medicine’s ability to treat a wide array of diseases is due mainly to the development of small-molecule drug discovery. Thousands of years of traditional medicines have been dwarfed in less than a hundred years. In addition, drug discovery has becoming a more consistent process with the introduction of the clinical trials process as well.Plasmodium is a genus of tropical parasites that have the potential to wreak havoc on billions of people globally. Current methods of drug discovery aimed at defeating these parasites require expensive assays that use radioactive material. By using an alkyne-labeled agent instead of a tritium-labeled agent, …

Withaferin A And Immune Checkpoint Blocker Therapy For The Treatment Of Non-Small Cell Lung Cancer, Roukiah Khalil

USF Tampa Graduate Theses and Dissertations

Lung cancer is the first cause of cancer-related deaths in both men and women with an overall five-year survival rate of 28%. Although immune checkpoint blockers (ICBs) are currently FDA-approved for the treatment of non-small cell lung cancer (NSCLC), only 17-20% of patients achieve durable responses by the induction of immunologic memory. The lack of response in most patients can be attributed to the tumor-intrinsic or tumor-extrinsic immune resistance mechanisms. A biomarker of importance is the Programmed Death Ligand-1 (PD-L1), as higher PD-L1 expression is usually associated with a better response to ICBs. Although studies have attempted to combine ICBs …

Acetate-Encapsulated Linolenic Acid Liposomes Reduce Sars-Cov-2 And Rsv Infection, Andrew R. Mcgill

USF Tampa Graduate Theses and Dissertations

Coronavirus disease-19 (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), is an ongoing pandemic-endemic. In the United States, it remains the third leading cause of death in 2022. SARS-CoV-2, an RNA virus, has a genome prone to rapid mutation, generating variant species capable of resisting therapies and vaccination efforts. However, it has been estimated that more than 40% of SARS-CoV-2 infections may be asymptomatic, which calls into question any biological differences between the asymptomatic and afflicted groups. Additionally, Respiratory Syncytial Virus (RSV) is the most significant cause of bronchiolitis and asthma in neonates, with an increasing burden on the …

Evaluation Of The Humoral Immune Responses To Plasmodium Vivax Circumsporozoite Protein (Csp)-Based Pre-Erythrocytic Vaccine Candidates, Jack Esquenazi

USF Tampa Graduate Theses and Dissertations

Malaria, caused by the apicomplexan Plasmodium spp. , is a major public health issue that impacts over one-third of the world’s population ,with Plasmodium vivax accounting for over 130 million clinical cases annually. The circumsporozoite protein (CSP) is the most abundant molecule on the surface of Plasmodium sporozoites and is considered a leading pre-erythrocytic stage vaccine candidate. CSP is essential for sporozoite maturation, migration, and invasion. Anti-CSP antibodies interrupt sporozoite migration and infection of hepatocytes thus reducing liver-stage burden. P. vivax CSP is composed of three subdomains: a highly polymorphic immunodominant central repeat region (CRR), conserved N-terminal ,and C-terminal subdomains. …

Development Of A Ddpcr Multiplex To Measure The Immune Response To Borrelia Burgdorferi., Kailey Marie Mccain

USF Tampa Graduate Theses and Dissertations

Zoonotic diseases account for a significant proportion of emerging infectious diseases (EIDs), with their dynamics being heavily influenced by the environment. While the effects of the environment on population-level disease dynamics have been extensively studied, there is limited research investigating the impact of the environment on individual-level disease susceptibility and transmission. This study aims to fill this gap by creating a tool to examine the variation in immune response to Borrelia burgdorferi, the causative agent of Lyme Disease, in two competent host species, Peromyscus leucopus and Peromyscus maniculatus. Two triplex droplet digital polymerase chain reaction (ddPCR) assays were developed to …

The Effects Of Integral And Incidental Emotions On The Perceived Risk Of Gun Violence, Gloria Laugalis

USF Tampa Graduate Theses and Dissertations

Fearful people are pessimistic when it comes to risk judgments and angry people areoptimistic in their risk judgments (Lerner & Keltner, 2000; 2001). Since this has been supported in studies regarding both incidental and integral emotions, the findings from incidental studies have been broadly generalized to answer research questions regarding integral emotions. This generalization was challenged when Ferrer and Ellis (2021) recently found differing effects of incidental and integral emotions of fear and anger. In this study, we examine the effects of these emotions, both incidental and integral to the target risk, on risk perception to help determine if incidental …

Preliminary Characterization And Functional Validation Of Pre-Erythrocytic Vaccine Candidates For The Malaria Parasite Plasmodium Vivax, Justin Lee Nicholas Iii

USF Tampa Graduate Theses and Dissertations

RTS,S/AS01, vaccine targeting malaria caused by Plasmodium falciparum, used in moderate to high malaria transmission, provides hope for pre-erythrocytic (PE) subunit malaria vaccine development. Despite this vaccine advance, efforts to develop an efficacious vaccine against the neglected parasite Plasmodium vivax have lagged. In addition, there exists a gap in our understanding of PE antigens that can induce broadly-neutralizing antibodies. In this dissertation, we provide the preliminary characterization of vaccine candidates targeting the PE of P. vivax. Through transcriptomic profiling data and a target product profile, we identified critical P. vivax antigens (SSP3, SPECT-1, and M2-MAEBL) that can potentially be targets …

Automated Approaches To Enable Innovative Civic Applications From Citizen Generated Imagery, Hye Seon Yi

USF Tampa Graduate Theses and Dissertations

Smart governance is an area, that is increasingly becoming important, not only in advanced countries, but all across the globe. Thanks to global scale network connectivity, permeance of smart-devices of various form-factors, and overall improvement in digital literary, we are now seeing "smartness" everywhere, or if not, the general public is expecting the same. Ultimately, the goal of smart governance is to facilitate state-of-the-art technologies to improve citizens’ lives. With the ubiquity of smart phone technologies today, citizens more readily participate in collaboration with public officials for improved quality of life and their communities. By utilizing optimal tools, public officials …

Data-Driven Learning Algorithm Via Densely-Defined Multiplication Operators And Occupation Kernels., John Kyei

USF Tampa Graduate Theses and Dissertations

Consider a nonautonomous nonlinear evolution $\dot{x}=f(x,t,\mu)$, where the vector $x(t) \in \mathbb{R}^n$ represents the state of the dynamical system at time $t$, $\mu$ contains system parameters, and $f(\cdot)$ represents a dynamic constraint. In most practical applications, the nonlinear dynamic constraint $f$ is unknown analytically. The problem of approximating $f$ directly from data measurements generated by the system is a main goal of this manuscript. In the postulates of the Nonlinear Autoregressive (NAR) framework, we show that the problem of approximating $f$ can be studied through symbols of densely defined multiplication operators over a Reproducing Kernel Hilbert Spaces (RKHS). In this …

A Forward Genetic Screen Identifies Genes Involved In Gametocyte Development Of The Human Malaria Parasite Plasmodium Falciparum., Jyotsna Chawla

USF Tampa Graduate Theses and Dissertations

Transmission of the deadly malaria parasite Plasmodium falciparum from human to mosquito is achieved by specialized intraerythrocytic sexual forms called gametocytes. Circulating in the vertebrate host, relatively few intraerythrocytic gametocytes are picked up during a bloodmeal to continue sexual development in the mosquito vector. Human-to-vector transmission thus represents an infection bottleneck in the parasite's life cycle for therapeutic interventions to prevent malaria. Though the crucial regulatory mechanisms leading to gametocyte commitment have recently come to light, networks of genes that control sexual development remain to be elucidated. Here, we report a forward genetic approach to identify genes associated with gametocyte …

Partial Characterization Of Plasmodium Falciparum Genes Implicated In Altered Artemisinin Stress Response, Caroline F. Simmons

USF Tampa Graduate Theses and Dissertations

Malaria is a parasitic illness caused by the Plasmodium spp., with an estimated 247 million malaria cases reported in 2021 across 84 endemic countries. Treatment of the deadliest malaria parasite, P. falciparum, relies on artemisinin combination therapies (ACTs). However, while ACTs have greatly decreased malaria mortality, this progress is threatened due to artemisinin (ART) partial resistance and ACT failure in Southeast Asia and Sub-Saharan Africa. SNPs to the Kelch13 (K13) gene are a well-characterized marker for ART resistance. However, increasing evidence that resistance to ART mediated by genes not related to K13 SNPs prompts the need to characterize other novel …

Histone Deacetylase 8 Is A Novel Therapeutic Target For Mantle Cell Lymphoma And Preserves Natural Killer Cell Cytotoxic Function, January M. Watters

USF Tampa Graduate Theses and Dissertations

This study demonstrates for the first time that HDAC8 function is critical for MCL survival, and abrogating its activity in human primary NK cells does not interfere with NK IgG antibody directed ADCC therapies ex vivo. Human NK cells, isolated from healthy donors, are highly resistant to HDAC8 inhibitor treatment with PCI-34051. Even at the highest concentration, 20uM, no toxicity was observed. Conversely, MCL cell lines representative of the aggressive MCL subtype, classical MCL, were especially sensitive to PCI-34051, an HDAC8 selective inhibitor, treatment. Blocking HDAC8 activity and/or abrogating expression through shRNA silencing induced significant DNA damage, hyperacetylation of SMC3, …