Digital Commons Network^™

Immune Modulation As A Therapeutic Target In An Α-Synuclein Model Of Parkinson’S Disease, Meena Subhashini Subbarayan

USF Tampa Graduate Theses and Dissertations

Parkinson’s disease (PD) is the second most common neurodegenerative disorder affecting about 1.5 million people in the United States with more than 60,000 people diagnosed each year. It is classically characterized by four major symptoms: tremor, postural instability, stiffness in joints, and slow movement (bradykinesia). Pathologically PD is characterized by up to 70% loss of dopaminergic neurons in substantia nigra pars compacta (SNpc) of midbrain and accumulation of presynaptic protein called α-synuclein (α-syn) within dopaminergic neurons that extend to the striatum. This disrupts the nigrostriatal pathway leading to the motor symptoms seen in PD patients. Microglia, the innate immune cells …

Bodily Harm: An Analysis Of The Phenomenological And Linguistic Aspects Of Harm And Trauma, Grant Samuel Peeler

USF Tampa Graduate Theses and Dissertations

This work seeks to explore the phenomenological experience of harm through an investigation of trauma and its existential features. Harm, despite its importance for many topics in both Political Science and Political Theory, is not often investigated as a subject in itself. By interrogating elements of Merleau-Ponty’s uniquely embodied philosophy, this work seeks to further our understanding of harm as a phenomenon which is both uniquely subjective and yet socially informed.

The text is split into two halves – with the first offering an exegesis of relevant sections of Merleau-Ponty’s Phenomenology of Perception, and the second engaging with contemporary secondary …

A Review Of American College Campus Tobacco Or Smoke Free Policies: A Case Study Of A Large Urban University, Sarah E. Powell

USF Tampa Graduate Theses and Dissertations

Objective: A year after a tobacco free policy was passed, the University of South Florida (USF) was interested in the overall policy opinions and efficacy. To assess this a project was completed that included a survey and geospatial data. The survey measured tobacco policy enforcement behavior and a geographic information system (GIS) mapped tobacco policy violation. This project introduces an evaluation process that can efficiently assess an institutions tobacco-free policy. Methods: Using a cross-sectional survey sent to students, faculty, and staff, understanding of policy and resources, tobacco use observations, stage of change regarding policy enforcement, self-efficacy to enforce, and perceived …

Humanistic Climate Philosophy: Erich Fromm Revisited, Nicholas Dovellos

USF Tampa Graduate Theses and Dissertations

Given the current juncture in history, humanity is faced with the herculean task of adapting to a tumultuous present and a gimmer future. Should climate projections be accurate, there is little time to waste. This work makes the claim that we are not only in a political gridlock but also in an academic one. Researching climate philosophy from its inception, the concluding view is that no major progress, outside of a standardized descriptive analysis, has been achieved. Thus, the work evaluates an array of climate philosophers e.g. Stephen Gardiner, James Garvey, Peter Singer, Walter Sinnott-Armstrong, etc., with specific emphasis on …

Using Geospatial Data To Predict The Locations Of Groundwater Discharge To Salmon-Bearing Streams, Alaska, Mary Gerlach

USF Tampa Graduate Theses and Dissertations

Identification and protection of groundwater resources are considerations of increasing interest as climate shifts but can be challenging to accomplish in remote areas. To that end, a series of GIS techniques and weight of evidence approach were applied to determine the feasibility of remotely identifying likely areas of ground discharge. Through the confluence of topographic analyses and a novel geologic dataset, these techniques were found to consistently identify areas characterized by either shallow subsurface or aquifer-fed groundwater discharge or evidence of ephemeral surficial water features. Two distinct GIS techniques to build spatial proxies of the effects of topography and geology …

Optimal Strategies For Grassland Restoration To Increase Spatial Carrying Capacity Of Florida Sandhill Cranes In Pasco County, Florida, Ibrahim Kaya

USF Tampa Graduate Theses and Dissertations

The Florida sandhill crane (Antigone canadensis pratensis) is a non-migratory bird species occurring throughout the Florida peninsula and the Okefenokee Swamp in southeastern Georgia. Human land-altering activities have led to a substantial decline in the amount and condition of suitable habitat, including grasslands, for the species in Florida, thereby decreasing the population size significantly. Grasslands located in close proximity to nesting locations are one of the habitat types on which the population viability of the species depends, as they provide foraging and brooding habitat which is crucial for successful reproduction. The purpose of this study is to propose the most …

A Sustainability Machine: The Incineration-Based Waste Regime In Tampa, Fl, Usa, Kevin P. Martyn

USF Tampa Graduate Theses and Dissertations

Recent history has seen some significant changes in terms of how society thinks about and deals with its wastes. Increasingly troubling indications of the immediacy of ecological concerns, now often described by the Anthropocene concept, have been provocative of a reinvigorated fervor for a sustainability transition. As a result, sustainability has become well-established as both an urgent pursuit and an eminently pliable buzzword. This research describes and explains one key aspect of society’s pursuit of some version of sustainability: our relationship with waste.

The site of this research is Tampa, Florida, a sunbelt city with a unique waste management system. …

Regulation Of The Heat Shock Response And Hsf-1 Nuclear Stress Bodies In C. Elegans, Andrew Deonarine

USF Tampa Graduate Theses and Dissertations

The Heat Shock Response (HSR) is a highly conserved stress responsive molecular pathway that functions to promote appropriate protein folding in the cell. The HSR accomplishes this primarily through the use of molecular chaperones that serve to bind to misfolded or unfolded proteins to assist in stabilizing and folding proteins back to their native functional state. The master regulator of this pathway is a transcription factor known as Heat Shock Factor 1 (HSF1). HSF1 regulates molecular chaperone expression in the cell’s basal state, but can also be stress induced by diverse biotic and abiotic signals including thermal shock, oxidative stress, …

Feeding Specificity Of The Photosynthetic Sacoglossan Sea Slug Elysia Papillosa, Kourtney Sui Barber

USF Tampa Graduate Theses and Dissertations

The sacoglossan sea slug, Elysia papillosa is associated with two species of the siphonaceous green alga, Penicillus, which co-occur in mixed macrophyte beds at Tarpon Springs, FL, USA. Field collections revealed that Elysia papillosa is frequently collected from the alga, P. capitatus, but is also present in much lower abundance on P. lamourouxii. Past studies have shown that some species of sacoglossans consume algal species that differ from the algae from which they are collected so, in order to determine whether E. papillosa was consuming either or both species of Penicillus, total DNA was extracted from individual slugs collected from …

Middle Woodland Mounds Of The Lower Chattahoochee, Lower Flint, And Apalachicola River Basin, Michael H. Lockman

USF Tampa Graduate Theses and Dissertations

A thorough literature review was completed in order to construct a database of all the known Middle Woodland-period (A.D. 300-700) mounds in the Apalachicola – lower Flint and lower Chattahoochee River drainages of northwest Florida, southwest Georgia, and southeast Alabama. The database presented in this document provides both quantitative and tabular data which may be used to evaluate, describe, and compare sites within this research region as they relate to one another as well as other sites in the Southeast. Through the lens of cultural materialism, descriptive statistics were used to summarize the accumulated data and ultimately refine our understanding …

Protease Resistant Growth Factor Formulations For The Healing Of Chronic Wounds, Tabitha Boeringer

USF Tampa Graduate Theses and Dissertations

Chronic wounds are those wounds which are refractory to treatment, often taking years to heal. These wounds are complex and may present with elevated protease levels, resulting in rapid degradation of growth factors, both native and augmented via treatment. This study’s focus was the development of a system to protect growth factors from protease mediated degradation.

The protective system consisted of chimeric fusion proteins developed to contain both a growth factor or known protease inhibitory peptide and an Elastin-Like-Peptide (ELP)component. The unique phase transitioning abilities of the ELP component allowed for the self-assembly of these chimeric fusion proteins into a …

Swimming Of Pelagic Snails: Kinematics And Fluid Dynamics, Ferhat Karakas

USF Tampa Graduate Theses and Dissertations

Pteropods (also known as sea butterflies or sea angels), are holoplanktonic marine snails which swim by flapping a pair of extremely flexible wings. The wings are modified from the molluscan foot and the wing motions are supported by the fluid pressure without any rigid support. Sea angels (gymnosome pteropods) are completely naked; in contrast, sea butterflies (thecosome pteropods) have negatively buoyant aragonite shells which vary in geometry and size among different species. Pteropods are seasonally abundant in the ocean, and an important food source for the other zooplanktons, fishes, and whales. Though studies have been conducted regarding their biology, ecology, …

Establishing Topological Data Analysis: A Comparison Of Visualization Techniques, Tanmay J. Kotha

USF Tampa Graduate Theses and Dissertations

When visualizing data, we would like to convey both the data and the uncertainty associated with it. There are many incentives to do this, ranging from hurricane path projection to geographical surveys. Important decision making tasks rely upon humans perceiving a clear picture of the data and having confidence in their decisions. Topological Data Analysis has the potential to visualize the data as features or hierarchies in ways that are familiar to human intuition, and thus could help us convey the variation associated with uncertainty.

In this thesis, we evaluate four visualization techniques: color maps, isocontours, Reeb graphs, and persistence …

The Impact Of Myeloid-Mediated Co-Stimulation And Immunosuppression On The Anti-Tumor Efficacy Of Adoptive T Cell Therapy, Pasquale Patrick Innamarato

USF Tampa Graduate Theses and Dissertations

Adoptive T cell therapy (ACT) in combination with lymphodepleting chemotherapy is an effective strategy to induce the eradication of tumors, providing long-term regression in cancer patients. However, only a minority of patients that receive ACT with tumor infiltrating lymphocytes (TILs) exhibit durable benefit. Thus, there is an urgent need to define strategies that potentiate anti-tumor activity conducted by adoptively transferred T cells. In these studies, we aimed to identify novel strategies to enhance the therapeutic efficacy of ACT. Accordingly, we describe the disparate roles of myeloid cells in the context of ACT characterized by the augmentation of TIL proliferation in …

Soil Characteristics Associated With Horse Cases Of Eastern Equine Encephalitis Virus In Florida, Fulya Guzelkucuk

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus (EEEV) is a mosquito-borne virus found in Florida that affects humans and horses. Also, EEEV is an agricultural land management issue in Florida, as it causes mortality in large numbers of horses each year and sometimes humans. This study investigates the characteristics of soils associated with horse cases of EEEV in the State of Florida, focusing on differences in soil characteristics between summer and winter cases. This study analyzed a total of 676 EEEV cases during 2005-2018, including 611 summer cases and 65 winter cases. Soil characteristics that were examined include: soil texture, drainage class, hydrology …

Super Bowl Ads And The Donald Trump Culture War, Jessica Barron

USF Tampa Graduate Theses and Dissertations

There have been studies that have looked at how television commercials represent or portray minorities such as gender or race. Very few have tried to look at how commercials compare to the culture, specifically in regards to a presidential term and the culture they promote. This present study examines Super Bowl commercials during the culture war led by the Donald Trump administration and looks to discover if these advertisements are becoming more diverse in comparison to the previous presidency. Using content analysis, the researcher analyzed and compared 50 commercials from three different time periods 2009-2010, 2013-2014, and 2017-2018. The categories …

Using A Systems Thinking Approach And Health Risk Assessment To Analyze The Food-Energy-Water System Nexus Of Seaweed Farming In Belize, Estenia J. Ortiz Carabantes

USF Tampa Graduate Theses and Dissertations

Certain macroalgae, commonly referred to as seaweed, have traditionally been used as a food source for many coastal communities around the world. In Belize, residents of the Placencia Peninsula cultivate two seaweed types, Eucheuma isiforme and Gracilaria spp. at Little Water Caye located 19 miles offshore. The dried seaweed is sold at the Placencia Producers Cooperative Society Limited (PPCSL) where community members process it into a variety of value-added products such as soaps, cosmetics, hair products, and food additives.

The overall goal of this research was to understand the existing food-energy-water system (FEWS) infrastructure landscape for seaweed production in Placencia, …

Roots Of Coded Metaphor In John Dee's Monas Hieroglyphica, Joshua Michael Zintel

USF Tampa Graduate Theses and Dissertations

An enormous amount of research on John Dee has materialized within the last forty years. Contrary to research published earlier in the twentieth century, such relatively recent studies have considered Dee’s idiosyncratic plurality of parallel traditions instead of trying to pigeonhole his activities into one of several discrete camps. That research (much of which is listed in the Bibliography) has been helpful hypothesizing what his Monas Hieroglyphica (1564) may mean for several fields of study in an interstitial capacity. Students of Early Modern mathematics, neoplatonism, and the histories of alchemy, chemistry, Christian kabbalah, and astronomy are among the many diverse …

A Chemical Investigation Of Three Antarctic Tunicates Of The Genus Synoicum, Sofia Kokkaliari

USF Tampa Graduate Theses and Dissertations

Marine invertebrates, mainly sponges, tunicates and corals, have in the past few decades attracted the interest of the scientific community in regard to their secondary metabolites and their potential as leads in drug discovery. The genus Synoicum is comprised of multiple organisms found in both deep and shallow waters, tropical and cold environments around the world. The majority of the members of this genus that have been investigated can be found in shallow tropical waters due to the ease of accessing and collecting them. Of the cold environments, Antarctica is a representative of the environments where members of the Synoicum …

Embodying The Empire: Imperial Women And The Evolution Of Succession Ideologies In The Third Century, Christina Hotalen

USF Tampa Graduate Theses and Dissertations

This dissertation traces the creation and negotiation of dynastic succession ideologies between the emperors and their subject populations between 193 and 313 CE, particularly through the advertisement of imperial women. Julia Domna, Otacilia Severa, and Galeria Valeria occupy watersheds in the evolution of third century dynastic succession ideologies. The administrations of each emperor crafted propaganda designed to elicit support for their reigns and dynastic ambitions, each tailored to appeal to a particular audience. Images of the empresses in official media were carefully constructed to elicit a population’s support for the emperor’s legitimacy. Subjects responded to these messages, seeking to have …

Facilitators And Barriers To Treatment Engagement In A Behavioral Parent Training Program, Holland Hayford

USF Tampa Graduate Theses and Dissertations

This quantitative study sought to analyze caregivers’ perspectives regarding facilitators of, and barriers to, attending the behavioral parent training programs (BPTs) known as Helping Our Toddlers, Developing Our Children’s Skills (HOT DOCS) and Developing Our Children’s Skills K-5 (DOCS K-5). Participants included 43 caregivers who successfully completed their respective DOCS program. These caregivers answered two questionnaires which contained items addressing demographic variables (i.e., gender, race, ethnicity, household income, level of education, number of children, and program attendance) and logistic factors of the DOCS programs (i.e., course location, class length, time of day, course duration, availability of transportation, availability of childcare). …

A Health Evaluation Of Gulf Of Mexico Golden Tilefish (Lopholatilus Chamaeleonticeps) And Red Snapper (Lutjanus Campechanus) Following The Deepwater Horizon Oil Spill, Kristina Leigh Deak

USF Tampa Graduate Theses and Dissertations

A lack of baseline heath indices for offshore Gulf of Mexico (GoM) teleosts complicated impact assessments of the Deepwater Horizon (DWH) oil spill. While measurement of contaminant levels in fish after a pollution event can document exposure, such data fail to provide meaningful information about how this contact affects an animal's physiology. Controlled exposure studies have highlighted the utility of biomarkers that may indicate deleterious, long-lasting effects of pollutant exposure on various life stages of fish, however, their extrapolation to wild-caught, non-model species is challenging. In an increasingly chemically-saturated environment, it can also be difficult to separate the influence of …

Development Of An Integrated Direct Membrane Filtration (Dmf) And Anaerobic Membrane Bioreactor (Anmbr) System For Dilute Municipal Wastewater Treatment, Ahmet Erkan Uman

USF Tampa Graduate Theses and Dissertations

The total electricity consumption due to municipal wastewater treatment in the US accounts for 1.3% of total demand. Also, a significant portion of biodegradable constituents in wastewater is converted into biomass, which needs further processing. On the one hand, an excessive amount of energy and chemicals are being used to treat wastewater, but on the other hand, resources (such as nitrogen and phosphorus, energy, and water) in wastewater are being discharged or removed rather than recovered. From this perspective, new wastewater treatment technologies are sorely needed to facilitate resource recovery from wastewater for a better, more sustainable future.

Anaerobic membrane …

Mechanistic And Translational Studies On Skeletal Malignancies, Jeremy Mcguire

USF Tampa Graduate Theses and Dissertations

New treatment strategies are desperately needed for treating skeletal malignancy. Skeletal malignancies can be either primary cancer that originated in the bone, such as osteosarcoma, or metastatic cancer that spread from another organ to the skeleton, as in the case of breast or prostate cancer. In this thesis, I will detail two projects that focus on the discovery of new treatment strategies for both primary skeletal malignancy and metastatic skeletal malignancy.

The first project focuses on the primary skeletal malignancy, osteosarcoma, a rare cancer that is commonly diagnosed in children and young adults and metastasizes to the lungs. The survival …

Evaluating The Effects Of Reinforcement Delay On Acquisition During Discrete Trial Training: A Literature Review, Theresa Barhold

USF Tampa Graduate Theses and Dissertations

This review of literature analyzes the use of immediate and delayed reinforcement within discrete trial training (DTT). Criteria for inclusion in this review consisted of articles published in English, human participants, single subject design, targeting skill acquisition in a DTT format, and inclusion of at least one delay to reinforcement condition. Four articles, with a total of 18 participants and 23 datasets, were identified in this review. Data on participants’ characteristics and procedural modifications (i.e., target response, type of prompt, delivery of praise, use of an error correction procedure, type of reinforcer, and delay to reinforcement) were extracted. Results indicate …

Biogas Upgrading Via Solid Resin-Based Amine Sorbents, Olusola Johnson

USF Tampa Graduate Theses and Dissertations

Biogas is a valuable renewable energy generated from anaerobic digestion of biodegradable organic matter. It is applicable as fuel in vehicles, for the generation of electricity, industrial heating, or as raw material to produce chemicals, liquid fuels, syngas, and compressed natural gas (CNG). Carbon dioxide (CO2) and methane (CH4) are the major components in biogas, with a trace amount of contaminants, including hydrogen sulfide (H2S), water vapor (H2O), nitrogen (N2), ammonia (NH3), oxygen (O2), carbon monoxide (CO), halides, volatile organic compounds (VOCs), siloxanes, and hydrocarbons.

The source of biogas, which is anaerobic digestion of different organic matter or landfill decomposition, …

Bio-Electrochemical Denitrification Systems And Applications For Nitrogen Removal In On-Site Wastewater Treatment, Kamal Ziad Taha

USF Tampa Graduate Theses and Dissertations

Denitrification experiments using biological cathodes (biocathodes) were conducted in three separate experiments. The first experiment was to observe whether the rate of denitrification in biocathodes could be increased with added ammonium at mass (mg/L) ratios of 0.100 (2.5/25), 0.05 (1.25/25), 0.0250 (0.625/25), and 0.0125 (0.3125/25) (parts ammonium to nitrate (NH4+-N/ NO3--N). The purpose of the ammonium present was to provide the microorganisms a starting nitrogen source for assimilation to biomass which, in theory, would encourage the removal of nitrate through dissimilatory denitrification. As mixed cultures were used, ratios tested were based on simplifying future operation of scaled-up biocathodes applied to …

Biological And Proteomic Characterization Of Cornus Officinalis On Human 1.1b4 Pancreatic Β Cells: Exploring Use For T1d Interventional Application, Arielle E. Tawfik

USF Tampa Graduate Theses and Dissertations

Type 1 diabetes (T1D) is an autoimmune disease resulting in the loss of pancreatic β cells and subsequent loss of insulin production. Exogenous insulin coupled with continuous glucose monitoring is the only treatment for T1D. Therefore, novel interventional therapies are needed that can inhibit the autoimmune destruction and increase β cell proliferation and function. Based on initial in vitro studies, traditional Chinese medicine (TCM) extract, Cornus officinalis, may be able to serve in this function. CO has been used for 2 millennia for its glucose regulating potential and has shown efficacy in animal models but rarely studied in the context …

An Analytical Approach To Information And Communication Technologies, Sahir Hismiogullari

USF Tampa Graduate Theses and Dissertations

The twentieth century was a century of scientific and technological development. Especially the fact of information and communication technologies, beyond being a technological leap, has led to different levels of development and changes / transformations. Therefore, developments, possibilities, changes and problems emerging in the Internet and other digital contexts have attracted the attention of communication scientists, sociologists, historians and philosophers, and have become a field of research that leads to interdisciplinary studies. Along with the mass media developed for the last two centuries (from the spread of newspapers to the invention of the internet), every social development or transformation is …

Polychlorinated Biphenyls, Organochlorine Pesticides, And Polycyclic Aromatic Hydrocarbons In Snapper (Family Lutjanidae) From Cuba And The Wider Gulf Of Mexico, Brigid E. Carr

USF Tampa Graduate Theses and Dissertations

The Snapper species assemblage is economically and environmentally important to all three countries bordering the Gulf of Mexico (GoM). Polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) were investigated in seven snapper species located in the GoM, with a specific focus on the those caught along the northwestern coast of Cuba. Hepatic samples were processed using QuEChERs extraction methodology and analyzed using gas chromatography with tandem mass spectrometry (GC-MS/MS). Nearly all fish included in this study contained detectable amounts of all three groups of these contaminants, regardless of location, and across all regions of the Gulf, indicating …