Digital Commons Network^™

Hyaluronic Acid Coated Targeted Lipid Micellar Nanoparticle As A Delivery Vehicle For Lapatinib And Ketoconazole In Egfr Mutated Lung Cancer, Nadia Tasnim Ahmed

USF Tampa Graduate Theses and Dissertations

Background: According to the American Cancer Society, lung cancer was accountable for over 142,000 deaths in the USA in 2019. Common mutations related to lung cancer mainly occur in TP53, EGFR, and KRAS genes. Lapatinib is a small molecule tyrosine kinase inhibitor which acts reversibly on both epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2). Overexpression of EGFR leads to increased production and signaling of proteins that cause upregulation of cellular proliferation, cholesterol synthesis and resistance to apoptosis. Ketoconazole is an imidazole antifungal agent which works principally by inhibiting the enzyme cytochrome P450 14α-demethylase (CYP51A1) …

Aligning Voice And Communication With Identity – A Survey On Transgender And Gender Diverse Populations, Rachel T. Chalom

USF Tampa Graduate Theses and Dissertations

Purpose: This research has two goals. The first goal is to examine transgender (TG) and gender diverse (GD) voice and communication functioning and the impact of voice on their everyday lives. The second goal is to examine the knowledge, attitudes, and beliefs speech-language pathologists (SLP) have about the TG and GD community and to investigate the relationship between self- and listener-perception and its correspondence to quality of life (QoL).

Methods: A total of 59 participants took part in this research. The research was separated into two parts, the first included 24 TG and GD individuals who participated in a Qualtrics …

Action Recognition Using The Motion Taxonomy, Maxat Alibayev

USF Tampa Graduate Theses and Dissertations

In the last years, modern action recognition frameworks with deep architectures have achieved impressive results on the large-scale activity datasets. All state-of-the-art models share one common attribute: two-stream architectures. One deep model takes RGB frames, while the other model is fed with pre-computed optical flow vectors. The outputs of both models are combined to be used as a final probability distribution for the action classes. When comparing the results of individual models with the fused model, it is common to see that that latter method is more superior. Researchers explain that phenomena with the fact that optical flow vectors serve …

The Effects Of Augmented Reality (Ar)-Infused Idiom Material On Iranian Students’ Idiom Achievements, Motivation, And Perceptions, Babak Khoshnevisan

USF Tampa Graduate Theses and Dissertations

Idiomatic competence poses a unique challenge on the second and foreign language learning process (Liontas, 1999). Multiple researchers have explored the perceptions and beliefs of language learners regarding idioms (Kömür & Çimen, 2009; Liontas, 2002a; Liontas, 2007). Other scholars have studied the role of technology in learning idioms (Amer, 2014; Andarab & Rouhi, 2014; Khoshnevisan, 2018a; Liontas, 2006). Additionally, researchers have widely investigated the impact of augmented reality (AR) on literacy development (Cheng & Tsai, 2014; Solak & Cakir, 2015). However, a thorough review of the related literature indicates few researchers have, to date, explored language learners’ perceptions about the …

A Visit To Cuba: Performance Ethnography Of Place, Adolfo Lagomasino

USF Tampa Graduate Theses and Dissertations

Bestowed to the Cuban government in 1956, The Parque Amigos de José Martí in Ybor City, FL is a historical site intended to symbolize the relationship between Tampa and Cuba that facilitated Cuba’s independence. Cuban cultural identity and the sense of Cubaness are confounded by the history of exile and the constraints of the United States Embargo. This project articulates the experience of the Cuban exile community and their descendants through descriptive accounts of visiting the Parque Amigos de José Martí. Visiting a place is framed as a means of identity performance and a method of performance ethnography, enabling discursive, …

Transcriptome Studies On The Toxicity Of Silica Nanoparticles, Shih-Yi Hsu

USF Tampa Graduate Theses and Dissertations

Nanotechnology enables more precise harmony in health condition via reducing the dosage amount, improving the delivery of hydrophobic drugs, more specific targeting to the cancerous sites, and so on. Nevertheless, issues regarding the toxicity of nanotechnology have begun to call for attention several decades later after the innovation of nanotechnology. Tools about risk management of nanotechnology have been developed, but recently not much evidence recognizes the toxicity of nanoparticles (NPs) except for some animal studies, which demonstrated organ damage after the exposure to NPs. Toxicogenomic approach refers to the method utilizing gene expression to evaluate the chemical toxicity. Databases as …

Automating The Classification Of Mosquito Specimens Using Image Processing Techniques, Mona Minakshi

USF Tampa Graduate Theses and Dissertations

According to WHO (World Health Organization) reports, among all animals, mosquitoes are responsible for the most deaths worldwide. Mosquito borne diseases continue to pose grave dangers to global health. In 2015 alone, 214 million cases of malaria were registered worldwide. According to Centers for Disease Control and Prevention (CDC) report published in 2016, 62,500 suspected case of Zika were reported to the Puerto Rico Department of Health (PRDH) out of which 29,345 cases were found positive. The year 2019 was recorded as the worst for dengue in South East Asia. There are close to 4,500 species of mosquitoes (spread across …

Exploring The Potential Of Nutrient Retention And Recycling With Wetlaculture Systems In Ohio With Physical And Landscape Models, Bingbing Jiang

USF Tampa Graduate Theses and Dissertations

Significant expansion of agricultural land use has been widely recognized for leading to global and regional negative environmental impacts, especially increased eutrophication of surface water systems for the last half-century. The landscape-scale environmental problem of overloading nutrients to lakes and streams by excessive fertilizer use and increased human-caused N-fixation is in urgent need of a sustainable landscape-scale solution. Wetlands have long been considered as an effective way to remove nutrients from surface water. However, the influence of regional seasonality and hydrologic conditions on agricultural runoff treatment wetlands still needs more investigation. A new approach, “wetlaculture,” has recently described as a …

The Immediate Effect Of A Brief Mindfulness Intervention On Attention And Acceptance, Xiaoqian Yu

USF Tampa Graduate Theses and Dissertations

Given the increased popularity of mindfulness in both the clinical settings and the general public, it is important to understand the active mechanisms of mindfulness. Mindfulness practice (MP) involves two active components, attention regulation and acceptance of experience, being aware of the current experience as it is without evaluating the experience as positive or negative. Much research has evaluated the attention regulation component and found that MP improves high-level (effortful) attention with few reported effects on low-level (automatic) attention. It is unclear whether MP affects merely low- or high-level attention, or both, because little empirical research has examined both low- …

Do Similar Exposure Groups (Seg) Differ From Air Force Base To Air Force Base? A Combat Arms Training And Maintenance (Catm) Noise Exposure Comparison Of Moody Afb And Macdill Afb., Miriam F. Escobar

USF Tampa Graduate Theses and Dissertations

Noise is a common hazard for the military. The Department of Defense has set an Occupational Exposure Limit for noise at 85 dBA. The Air Force manages exposures using similar exposure groups (SEGs) which are formed at each Air Force Base. The purpose of this study was to compare the noise levels in SEGs in two Air Force bases across distance and time, and to review the effectiveness of the Air Force Hearing Conservation Program over distance and time. The SEGs chosen were Combat Arms Training and Maintenance (CATM) at Moody Air Force Base and MacDill Air Force Base because …

Non-Invasive Sex Determination And Genotyping Of Transgenic Brugia Malayi Larvae, Santiago E. Hernandez Bojorge

USF Tampa Graduate Theses and Dissertations

Lymphatic filariasis is a very painful and disfiguring helminth disease caused by the tissue nematodes Wuchereria bancrofti, Brugia malayi, and Brugia timori. This parasitosis is considered a Neglected Tropical Disease and it is a major public health burden for 72 tropical countries of Africa, Southeast Asia, the Caribbean, and South America. Despite the effectiveness of many control programs, there remains the need to develop new pharmacological agents to treat lymphatic filariasis, as most programs rely on a limited variety of drugs that are expensive, logistically difficult to obtain, and can lead to drug resistance. The Clustered Regularly Interspaced Short Palindromic …

Ghostly Entanglements: Exposing Temporal Matter(Ing)S In Social Studies Education With (Re)Photography, Bretton A. Varga

USF Tampa Graduate Theses and Dissertations

Visual resources offer a variety of pedagogical advantages. Regardless of content, images can be leveraged to build critically analytical skills and used to cultivate dialogic literacies (i.e., articulations of thinking) about the social world. Further, images can be used to introduce and grapple with complex/abstract ideas. Given the relationship that social studies has to temporality (i.e., subjective engagements with the past, present, and future), visual resources also present teacher(s) (educators) with the opportunity to explore (theoretical) concepts foregrounding manifold relationships making up the social world (i.e., a world consisting of complex entanglements between non/human entities).

The purpose of this research …

Anorexia Nervosa And Bulimia Nervosa As Expressions Of Shame In A Post-Feminist, Emily Kearns

USF Tampa Graduate Theses and Dissertations

Anorexia nervosa and Bulimia nervosa are heavily gendered conditions affecting women to a far greater degree than men in a post-millennium Western setting. The psychologistic and medicalized approaches to studying and treating these disorders do not account for socio-cultural and epistemic preferences. This paper draws a connection between shame (as emotion and affect) and these gendered disorders. Further, this work analyzes neo-liberalism, post-feminism, and consumerism as predatory elements of Western culture especially affecting women.

Social Exclusion Of Older Mossi Women Accused Of Witchcraft In Burkina Faso, West Africa, Clarisse Barbier

USF Tampa Graduate Theses and Dissertations

Among the most marginalized populations in the world, one group of women has been persistently ignored, silenced, and forgotten. In Burkina Faso, West Africa, older women in rural villages are often the target of witchcraft accusations; the consequences of these accusations are alarming because these women undergo violent attacks, face exclusion from their villages, and become the most vulnerable and marginalized segment of the Burkinabe population. Between August 2017 and November 2018, I conducted an ethnographic study of Burkinabe women accused of witchcraft living in two shelters in the capital city of Ouagadougou and examined women’s experiences of accusation, trauma, …

Overturning The Turnbull Settlement: Artifact Analysis Of The Old Stone Wharf In New Smyrna Beach, Florida, Tracy R. Lovingood

USF Tampa Graduate Theses and Dissertations

This thesis is a report on an internship at the New Smyrna Museum of History, which primarily focused on the analysis of artifacts found at the primary import and export site of the Turnbull Settlement, called the Old Stone Wharf (8VO4298). The Turnbull settlement in New Smyrna Beach, Florida, was a plantation settlement founded by Dr. Andrew Turnbull of England, using the labor force of primarily European indentured servants. The settlement only lasted from 1768 to 1777. After it failed, all the inhabitants moved 75 miles north to St. Augustine and were able to flourish there, leaving a descendent community …

From Colonial Legacy To Difficult Heritage: Responding To And Remembering An Gorta Mór, Ireland’S Great Hunger, Katherine Elizabeth Shakour

USF Tampa Graduate Theses and Dissertations

This research has two main components: first, an exploration of how communities react to socio-natural disasters through time, and second, a discussion of how communities constructed the responses to tragedies as heritage over the long term. Disasters are often conceived as short-term, natural catastrophes, but, in reality, they are always social and natural phenomena and often impact communities for years or even decades. Employing archaeological, historical and ethnographic methods, this project examines local, regional, and national responses to social upheaval cause by prolonged food insecurity beginning with a potato blight in 1845 in Ireland. The 1845-1850 Famine was not just …

Simulation And Verification Of Phase Change Materials For Thermal Energy Storage, Marwan Mosubah Belaed

USF Tampa Graduate Theses and Dissertations

Greenhouse gas (GHG) emissions have a global impact on world economies and the ecosystem. Scientific assessment of GHG emissions is an issue that requires immediate action from key decision-makers and influencers. A comprehensive review of the impact of climate change and its dire consequences on daily life is illustrated in this dissertation. The most common technique to lower GHG emissions in the atmosphere is by switching GHG emission sources to zero-emission sources such as solar energy, wind energy, bioenergy, and geothermal power. Recently, all these technologies have made significant progress in lowering capital operation costs and their cycle efficiencies. This …

Laser Micropatterning Effects On Corrosion Resistance Of Pure Magnesium Surfaces, Yahya Efe Yayoglu

USF Tampa Graduate Theses and Dissertations

Magnesium and its alloys are good candidates to manufacture medical implants. They have excellent biocompatibility and because they biodegrade secondary surgical operation to remove the implant could be eliminated. However, in aqueous environments, magnesium alloys rapidly corrode, resulting in premature degradation of the implant along with biologically intolerable hydrogen gas generation. In literature, there are multiple studies focused on creating water repelling hydrophobic magnesium surfaces in order to decrease corrosion rates. Hydrophobic properties can be achieved by creation of a roughness profile on an initially smooth surface combined with a treatment that reduces the free surface energy. In theory, hydrophobic …

Study Of The Therapeutic Effects Of Synchronization-Modulation Of The Na/K Pump On Muscle Fatigue, Jason E. Mast

USF Tampa Graduate Theses and Dissertations

It has been shown that by applying a specially designed oscillating external electric field to a cell membrane that the membrane's sodium/potassium pumps can be synchronized to all work at the same rate. Then by slowly increasing the electric field's frequency the pumps' turnover rate can also be increased. By increasing the pumps' turnover rate, the sodium and potassium concentration gradients can be increased, this type of stimulation is called synchronization-modulation. There are three generations of the synchronization-modulation waveform each with different utilities. In particular, the third generation of synchronization-modulation has the ability to use the energy of the external …

Hybrid Electro-Plasmonic Stimulation Of Primary Neurons, Ratka Damnjanovic

USF Tampa Graduate Theses and Dissertations

Biomedical prosthetics utilizing electrical stimulation have limited, effective spatial resolution due to spread of electrical currents to surrounding tissue, causing nonselective stimulation. So, precise spatial resolution is not possible for traditional neural prosthetic devices, such as cochlear implants. More recently, alternative methods utilize optical stimulation, mainly infrared, sometimes paired with nanotechnology for stimulating action potentials, which has its own drawbacks, as it may heat surrounding tissue. Recently, we employed plasmonic stimulation methods utilizing gold nanoparticle-coated nanoelectrodes to convert visible light pulses into localized surface plasmon resonance transduction for stimulation of electrically excitable cells, which had limited success. Here, we report …

Allosteric Control Of Proteins: New Methods And Mechanisms, Nalvi Duro

USF Tampa Graduate Theses and Dissertations

Allostery describes the phenomenon where perturbations in one region of a protein affect protein behavior in another non-overlapping region. Considerable efforts made over decades to understand the molecular basis of allostery, yet an overarching theory that can predict signaling pathways and contributions from chemical components is still lacking. In fact, molecular details in even the most well-studied of model systems, PDZ domains and GPCRs, remain unclear. In this dissertation I use molecular simulation methods to understand the role of allostery in the regulated entry of paramyxovirus into host cells, and also develop a new method to determine time-dependent signaling pathways …

Auditory Perception And Neural Encoding Of Spectral Modulation, Sittiprapa Isarangura

USF Tampa Graduate Theses and Dissertations

Most natural acoustic sounds consist of multiple frequencies. An important auditory perceptual ability to resolve such acoustic properties is referred to as spectral processing and includes frequency resolution, frequency selectivity and spectral envelope perception. Frequency resolution, as demonstrated by masking techniques, indicates that our auditory system functions as an auditory filter bank with overlapping bandpass filters. These filters allow for the effective separation of sounds into different frequency bands or channels. Acoustic components that fall within a given filter or passband are considered to be processed “within channel” and are perceptually resolved using “local” spectral cues, whereas components in different …

The Threat Of Virality: Digital Outrage Combats The Spread Of Opposing Ideas, Curtis Puryear

USF Tampa Graduate Theses and Dissertations

The prevailing stage for conversations about politics and morality has shifted from private and face-to-face to public and digital. Moreover, the digital landscape itself changed considerably in the past decade. The era of static webpages has been replaced by dynamic social networks where ideas and reactions to events spread rapidly. With every comment we, or a political adversary makes, numbers quantifying social approval tick up or down. Instead of holding digitized versions of one-on-one conversations, we argue in front of audiences who throw digital “points” at and accelerate the spread of the winning side’s ideas. I argue this subjectively raises …

Understanding The Role Of Cereblon In Hematopoiesis Through Structural And Functional Analyses, Afua Adutwumwa Akuffo

USF Tampa Graduate Theses and Dissertations

The discovery and implementation of immunomodulatory drugs (IMiD®s) has revolutionized the treatment of many hematological malignancies due to the plethora of IMiD®-induced clinical responses that include anti-angiogenesis, anti-inflammation, and anti-tumor effects, as well as enhanced erythropoiesis, immune modulation and improved metabolism. More ground-breaking was the identification of cereblon as the target of IMiD®s. Upon binding to thalidomide and other immunomodulatory drugs, the E3 ligase substrate receptor cereblon (CRBN) promotes proteosomal destruction of neo-substrates by engaging the DDB1-CUL4A-Roc1-RBX1 E3-ubiquitin ligase in human cells but not in mouse cells suggesting that sequence variations in CRBN may cause its inactivation. Therapeutically, CRBN engagers …

Orally Administered Nanoscale Drug Delivery System For Colorectal Cancer, Bhavya Shah

USF Tampa Graduate Theses and Dissertations

Mithramycin-A (Mit-A) is a US Food and Drug Administration (FDA) approved chemotherapeutic agent that is used for the treatment of testicular cancer. According to previous studies, Mit-A has shown promising results in targeting colorectal cancer (CRC) and cancer stem cells (CSCs) which are responsible for a highly invasive and metastatic forms of CRC. The use of Mit-A as chemotherapy is limited in CRC due to its toxicity. To minimize its toxicity, we have developed an orally delivered nanoscale drug delivery system by which Mit-A can be delivered using biocompatible and biodegradable materials. Mit-A nanoparticles were prepared using Poly(lactic-co-glycolic acid) as …

Polycyclic Aromatic Hydrocarbon Exposure, Hepatic Accumulation, And Associated Health Impacts In Gulf Of Mexico Tilefish (Lopholatilus Chamaeleonticeps), Susan M. Snyder

USF Tampa Graduate Theses and Dissertations

Following the 2010 Deepwater Horizon oil spill, systematic demersal longline surveys were conducted throughout the Gulf of Mexico (GoM) continental shelf to evaluate polycyclic aromatic hydrocarbon (PAH) exposure, hepatic accumulation, and health indices in demersal fishes. Tilefish (Lopholatilus chamaeleonticeps) were chosen as a target species due to high vulnerability to environmental disturbance, commercial importance, Gulf-wide distribution, and documented high exposure to PAHs post-Deepwater Horizon. Over 200 Tilefish were sampled in the north central GoM at repeat stations from 2012 to 2017, and from the northwest GoM, southwest GoM, Bay of Campeche, and Yucatán Shelf over years 2015 and 2016. Tilefish …

Investigating Mechanisms Of Immune Suppression Secondary To An Inflammatory Microenvironment, Wendy Michelle Kandell

USF Tampa Graduate Theses and Dissertations

The immune system plays a dynamic role in cancer progression. The theory of immunoediting suggests that the relationship between the tumor cell and the immune cell is one that is in flux: initially highly active and responsive to tumor antigen to one that has escaped immune responsiveness. Once the tumor has formed and effectively “escaped”, there are multiple mechanisms that work against a conventional immune response. The tumor cell clones that have escaped the elimination phase are those that are less immunogenic. These clones downregulate MHC, have increased apoptosis through DAMP-driven mechanisms, and suppressive cell phenotypes are driven through cytokine-driven …

A Novel Intramolecular Interaction In P53, Fan He

USF Tampa Graduate Theses and Dissertations

The p53 tumor suppressor is a sequence-specific DNA binding protein that activates gene transcription to regulate cell survival and proliferation. The activation process involves post-translational modifications that suppress p53 degradation by MDM2 and increase p53 DNA binding affinity. p53 is mutated in ~50% of human tumors, with higher frequency in specific tumor types and after relapse. Mutated p53 loses transcriptional activity and gains new functions that drive tumor progression. Both N-terminus (NT) and C-terminus (CT) of p53 contain intrinsically disordered regions. The p53 CT has well-documented effects in regulating DNA binding. CT truncated p53 mutants showed defective DNA binding and …

Development Of A Monolithic Implantable Neural Interface From Cubic Silicon Carbide And Evaluation Of Its Mri Compatibility, Mohammad Beygi

USF Tampa Graduate Theses and Dissertations

One of the main issues with micron-sized intracortical neural interfaces (INIs) is their long-term reliability, with one major factor stemming from device material failure caused by the heterogeneous integration of multiple materials used to realize the implant. Single crystalline cubic silicon carbide (3C-SiC) is a semiconductor material that has been long recognized for its mechanical robustness and chemical inertness. It has the benefit of demonstrated biocompatibility, which makes it a promising candidate for chronically-stable, implantable INIs. In the first section of this dissertation, the fabrication and initial electrochemical characterization of a monolithic, Michigan-style 3C-SiC microelectrode array (MEA) probe is reported. …

Large Thecosome Pteropods Of The Northern Gulf Of Mexico: Species Abundance, Spatial And Vertical Distribution With A Temporal Comparison Of Shell Thickness, Sarah M. Shedler

USF Tampa Graduate Theses and Dissertations

Large thecosome pteropods have a significant role in the pelagic ecosystem of the northern Gulf of Mexico (GoM). This research analyzed species abundances, vertical and horizontal distributions, and trends in shell thickness between 2011 and 2015. Pteropod samples were collected following the 2010 Deepwater Horizon oil spill by two midwater sampling programs: the Offshore Nekton Sampling and Analysis Program (ONSAP, 2011) and the Deep Pelagic Nekton Dynamics of the Gulf of Mexico (DEEPEND, 2015). All samples were collected using a 10-m2 Multiple Opening/Closing Net and Environmental Sensing System (MOC10) midwater trawl, with 3-mm mesh size. This gear sampled five discrete …