Digital Commons Network^™

Dendriplex - Cyclodextrin Conjugates For Gene Delivery To Retina, Durga Deepak Puro

USF Tampa Graduate Theses and Dissertations

Gene therapy holds promise for treating a wide range of diseases including cancer and several other polygenic and monogenic disorder arising in different organs. Due to its immune privilege nature, eye is an ideal organ for gene therapy,as various visual pathologies arise from gene defect and can lead to partial or complete vision loss. The last decade, several nanoparticles have been developed and tested for their transfection efficiency in vitro and in vivo. Among them, dendrimers with small size, good physicochemical properties and endosomal escape activity have shown high transfection efficiency in various cell lines. In an attempt to further …

Data-Driven Modeling Of The Causes And Effects Of Interneuronal Dysfunction In Alzheimer’S Disease And Dravet Syndrome, Carlos Perez

USF Tampa Graduate Theses and Dissertations

One of the defining features of Alzheimer’s disease (AD) is the increased cleavage of the amyloid precursor protein (APP), causing abnormally high levels of the aggregation form of amyloid beta (Aβ ). Many studies have shown that both AD patients and AD mice models exhibit abnormal network activity, including hypersynchronous excitatory neuron behavior, altered brain rhythms, and in some instances epileptic seizures when exposed to high levels of Aβ In particular, strong experimental evidence suggests that it is the small globular amyloid oligomers (gOs) and curvilinear fibrils (CFs) rather than the more stable, late stage rigid fibrils (RFs) that cause …

Isolation Of Specific Phospholipids From Nannochloropsis Oculata Microalga For Cosmetic Applications, Ahmet Yener Manisali

USF Tampa Graduate Theses and Dissertations

Algae, photosynthetic aquatic organisms, have recently caught the attention of the food, cosmetic, pharmaceutical, and nutraceutical industries due to the variety of natural compounds in their cellular bodies such as carbohydrates, lipids, and proteins. Specifically, microalgae-derived natural compounds such as phospholipids are extensively utilized in cosmetics as part of liposome formers, emulsifiers, solubilizers, and wetting agents. Although phospholipids are currently extracted from food sources, this practice raises sustainability concerns. Hence, growing microalgae on the provision of macronutrients such as nitrates and phosphates will serve as a more sustainable source of the several specific phospholipids such as phosphatidylcholine (PC), phosphatidyl-ethanolamine (PE), …

I'M Going Digital: Potentials For Online Communities Through Internet Remix, Justin N. Nguyen

USF Tampa Graduate Theses and Dissertations

In the late 90s and early 2000s, computers and internet usage became more widespread. This widespread usage led to a proliferation of “digital objects” (digital versions of audio, video, image, text, etc.) dispersed through the internet. The instant and near-infinite reproducibility of digital objects combined with their ease of manipulation by computer users has led to their widespread recognition and use as tools of communication online. As a consequence, technology has allowed those traditionally thought of as consumers to become cultural producers by repurposing digital media objects. Because of this, internet remix of digital objects is one of the best …

Modeling Early Life: Ontogenetic Growth And Behavior Affect Population Connectivity In Gulf Of Mexico Marine Fish, Kelly Vasbinder

USF Tampa Graduate Theses and Dissertations

This dissertation is an examination of growth, behavior, and dispersal during the early life stages of marine fishes in the Gulf of Mexico. Understanding movements of early life stages is a key part of managing exploited fish populations. Position in the water column can impact larval dispersal, since it determines those currents to which larvae are exposed. First, I investigated the relationship between length and age in early life stages of marine fishes. I found that demersal fish taxa tend to be represented by exponential models, while pelagic fish tend to be represented by linear models. I suggest this may …

Characterizing Childhood And Diet In Migration Period Hungary, Kirsten A. Verostick

USF Tampa Graduate Theses and Dissertations

This project investigates children, childhood and diet of two different Migration Period (4^th-8^th century AD) populations, the Gepids and the Avars, in the Great Hungarian Plain. The main goal was to assess whether there are differences in treatment of children and differences in breastfeeding and weaning practices in these distinct sites and populations. Secondarily, this research also focused on characterizing diet for the Gepids and the Avars at four different sites from the Migration Period, to understand how the migration and settling into the region and the assimilation of other groups into the two populations affected their …

Hearing Aid Acclimatization In The Context Of Central Gain Mechanisms, Peter J. Hutchison

USF Tampa Graduate Theses and Dissertations

The primary goals of this dissertation were to 1) identify markers of central gain in the auditory system following short-term acoustic attenuation via earplugging and acoustic enhancement via sound generators, and 2) determine if acoustic enhancement is effective in modulating central gain in older adults with age-related hearing loss, and if so, to what extent. Additionally, a further goal of this dissertation was to explore the possibility that central gain is related hearing aid acclimatization. The results described in Chapter 2 are further evidence that altering acoustic input to the peripheral auditory system modulates central auditory plasticity and is evident …

Cross-Host Correlations And Multivariate Effects Of Herbivore Specialization, Daniel J. Zydek

USF Tampa Graduate Theses and Dissertations

The distribution of insect herbivores among plant hosts is largely nonrandom: most herbivores have limited sets of hosts within one or a few plant families. This host use specialization is reinforced by traits that confer differential fitness across host plant species. Classic explanations for herbivore specialization predict that evolutionary trade-offs reinforce these relationships by imposing costs in the form of reduced potential fitness on alternative hosts, due to negative genetic correlations in fitness across hosts. This prediction that trade-offs constrain host use in herbivores can be tested with experimental evolution, by showing the direct evolutionary effects of host manipulation on …

Design And Implementation Of Solid/Solid Phononic Crystal Structures In Lateral Extensional Thin-Film Piezoelectric On Silicon Micromechanical Resonators, Abdulrahman Alsolami

USF Tampa Graduate Theses and Dissertations

Achieving high quality factor in MEMS resonator devices is a critical demand for today’s wireless communication and sensing technologies. In order to reach this goal, several dedicated prior works have been conducted based on published literature at different frequency ranges. Particularly, piezoelectrically transduced resonators, which are widely deployed in commercial wireless communication systems, could benefit from greatly improved qualify factor. So far, their development has evolved from thin film bulk acoustic resonators (FBAR’s) using surface attached piezoelectric thin-film transducers with moderate Q factors to high Q resonators equipped with a side-supporting tether (anchor) attached vibrating resonators that allow the devices …

Networks In The Norm Life Cycle And The Diffusion Of Environmental Norms, James E. Fry

USF Tampa Graduate Theses and Dissertations

In this research, I analyze how Transnational Municipal Networks (TMNs) and cities affect the diffusion and transmission of a decarbonization norm. Urban policy and political science scholars assert that cities and their networks are influential in implementing internationally coordinated environmental policy. However, few projects have analyzed how local actors may diffuse environmental norms that have been developed in the international system. Using the norm life cycle, this research explores the transmission of a decarbonization norm by means of GHG measurement and mitigation. I identify two critical objectives associated with a decarbonization norm: establishing a system for monitoring GHG emissions and …

Spontaneous Raman Scattering Enhancement With Microcavities And Multipass Resonators For Trace Gas Detection, Juan Sebastian Gomez Velez

USF Tampa Graduate Theses and Dissertations

The continual increase in production and use of chemicals in an ever-growing field of applications naturally brings forth the necessity to accurately and efficiently measure molecular composition. Spontaneous Raman scattering is a reliable technique which can optically identify molecules based on their intrinsic rotational-vibrational energy structure. The Raman emission from a substance can be spectrally analyzed to detect molecular species simultaneously and with isotopic sensitivity using a single laser source. However, even though the process is non-invasive and effective, the rate at which the emission occurs is notoriously low due to a weak scattering cross-section. Therefore, research into the development …

Environmental Influences On Expression Of Virulence- And Survival-Associated Genes And Epigenetic Modifications Of Dna In Vibrio Vulnificus, James W. Conrad

USF Tampa Graduate Theses and Dissertations

Vibrio vulnificus is an autochthonous inhabitant of warm, brackish coastal waters and is an opportunistic pathogen that causes the highest mortality of all seafood-related illnesses (1). These fatal infections are generally caused by the clinically-associated vcgC/16S rRNA type B genotypes within the biotype I group (2–5). However, the reasons for the heightened infectiousness of the clinically-associated strains over environmentally-associated ones remains elusive, as no unique clinically-associated virulence genes have been identified through genomic sequencing or other strategies. DNA methylation may contribute to regulation of virulence by affecting gene transcription, and was investigated in the highly virulent V. vulnificus strain CMCP6. …

Reproduction And Parasite-Mediated Selection, Meredith A. Krause

USF Tampa Graduate Theses and Dissertations

Sexual reproduction generates genetic diversity that can help hosts respond to selection by parasites, and in this thesis, I test three predictions on how reproduction impacts predictions by the parasite theory of sex and the Red Queen hypothesis. In Chapter one, using a meta-analysis, I found that asexuals (lower genetic diversity) have more parasites than sexuals (higher genetic diversity), but this difference can be heavily mediated by the mode and origin of asexuality. Further, hybridization but not polyploidy can blunt predicted differences in parasite loads among sexual and asexual hosts. In Chapter two, I flip the perspective of Red Queen …

Spatial And Temporal Determinants Associated With Eastern Equine Encephalitis Virus Activity In Florida, Kristi M. Miley

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus (EEEV) is considered the most pathogenic mosquito-borne illness in the United States. Human mortality has been estimated to range between 35 to 75%, with horses experiencing mortality rates greater than 90%. A large number of EEEV cases occur in Florida. Though we have come a long way since the first human infection was identified in the 1930s, there is still much to learn regarding the virus’s ability to maintain transmission year-round in Florida. Phylogenic studies support that Florida may serve as the geographic reservoir for EEEV. This research investigated spatiotemporal and ecological variables associated with risk …

Evaluation Of Aging And Genetic Mutation Variants On Tauopathy, Amber M. Tetlow

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is characterized by amyloid β plaques and neurofibrillary tau tangles (NFTs). While research has demonstrated amyloid pathology occurs prior to tau pathology, or tauopathy, tau has proven to be more toxic. Tauopathy is associated with cognitive declines and neurodegeneration. These findings have highlighted the importance of further understanding tauopathy. In the progression of tauopathy, there is an observable immune response that can be measured by glial cells such as microglia. Activated microglia are known to exacerbate tauopathy rather than reducing the pathology. Research has indicated that with increased age there is an increased risk for AD-related tauopathy …

“We Developed Solidarity”: Family, Race, Identity, And Space-Time In Recent Multiethnic U.S. American Fiction, Kimber L. Wiggs

USF Tampa Graduate Theses and Dissertations

In Diversity in Families, sociologists Maxine Baca Zinn, D. Stanley Eitzen, and Barbara Wells assert, “At a very personal level, families are crucial shapers of who we are and what our opportunities have been and will be” (xvii). The novels in this dissertation—Octavia Butler’s Kindred (1979), Karen Tei Yamashita’s Tropic of Orange (1997), and Rosaura Sánchez and Beatrice Pita’s Lunar Braceros 2125-2148 (2009)—examine the role of family in the development of individual identity and the practice of social justice. These authors foreground characters from various ethnic backgrounds and depict how the characters form new, multiethnic families. My dissertation explores the …

Save Water Drink Wine: Challenges Of Implementing The Ethnography Of The Temecula Valley Wine Industry Into Food-Energy-Water Nexus Decision-Making, Zaida E. Darley

USF Tampa Graduate Theses and Dissertations

This study demonstrates the interrelationships of people, food, energy, and water associated with Temecula Valley’s wine industry and reveals contradictions and biases in how people view these resources, which ultimately shape management and policies. The FEW (Food, Energy, and Water) Nexus is an approach increasingly used by policy- and decision-makers to understand the interrelationship of several resources. However, a FEW Nexus approach often lacks in social aspects that influence environmentally and economically sustainable outcomes, especially in the wine and wine tourism industry. When quantitative and qualitative data are available, the other challenge is which assessment to use. Two assessments often …

Coral Reef Restoration In The Tropical West Atlantic Amid The Covid-19 Pandemic, Linden Cheek

USF Tampa Graduate Theses and Dissertations

Climate change is increasing threats to coasts, both from storm surge and sea level rise. Healthy coral reefs provide reduction in storm surges, wave energy, coastal flooding and everyday erosion, and are found across a variety of spatial scales. Given the state of coral reefs worldwide, active Coral Reef Restoration (CRR) in emerging as a necessary component of coastal protection. CRR can be classified as a nature-based solutions (NbS) for coastal protection that also provides a multitude of ecosystem-based services to both humans and other life. Nearly all literature on coral restoration efforts assume a steady-state of human-ecological interactions, but …

Feasting On Words: What University Students Learn When They Study Food Writing And Food Media, Janet K. Keeler

USF Tampa Graduate Theses and Dissertations

The use of food in college curriculum is unique in its ability to create lasting impact because of the keen interest millennial and Generation Z students have in what they eat and drink. Studying media with food at its core is an underutilized mechanism to show how food intersects with the lives of all people thus encouraging students to look beyond their own experiences to consider the wider society. A program evaluation of 10 semesters of food writing and food media courses at a Florida public university reveals the ways in which students make deeper connections to culture and current …

Spatial Stereo Sound Source Localization Optimization And Cnn Based Source Feature Recognition, Cong Xu

USF Tampa Graduate Theses and Dissertations

In the process of propagating as a carrier of information in space, in addition to transmitting the information itself, the acoustic signal also contains the position information of the sound source itself and its related physical characteristics. Acoustic signal uses the medium (such as air, water, steel, etc.) in the space to transmit mechanical vibration and longitudinal waves from the sound source to the outside world. The traditional single audio collection device cannot collect position information and sound source characteristic information. Therefore, the signals processed in the audio signal processing process are all mixed source acoustic signals after spatial reverberation. …

Assessment And Integration Of Socioeconomic And Demographic Factors In The Implementation Of Integrated Water Resources Management In The Lake Chad Basin, Nodjidoumde Mbaigoto

USF Tampa Graduate Theses and Dissertations

Most developing countries have responded to the 2002 World Summit on Sustainable Development (WSSD) call to implement Integrated Water Resources Management (IWRM) in their national water policy to reach water sustainability and socioeconomic welfare. Despite the Lake Chad Basin Commission's adoption of the IWRM principles, the basin experiences a massive humanitarian crisis driven by the lake's shrinkage, the water shortage for food production, the rapid population growth, and the terrorist attacks perpetrated by Boko Haram. This study sought to assess the socioeconomic and demographic status of people living upstream of Lake Chad in order to understand the challenges associated with …

A Review Of Antecedent- And Consequence-Based Interventions For Vocal Stereotypy, Christine A. Roeper

USF Tampa Graduate Theses and Dissertations

The exact prevalence of vocal stereotypy within individuals diagnosed with ASD in currently unknown. In a 2011 study on symptoms of ASD by Mayes and Calhoun, 85% of parents reported their child engaged in repetitive, atypical speech or vocalizations. While there is a plethora of research on behavioral interventions to reduce levels of vocal stereotypy in individuals diagnosed with Autism Spectrum Disorder, at the time this review was written there is only one published review of the literature written by Lanovaz and Sladeczek in 2011. Therefore, the purpose of this paper is to extend and update the 2011 review conducted …

Dentro Y Fuera: La Obra De William Navarrete En Diálogo Con La Tradición Literaria Cubana, Clara Olivia Ocampo Zaldivar

USF Tampa Graduate Theses and Dissertations

En este trabajo se explora la visión de William Navarrete sobre Cuba y los recursos que utiliza, así también cómo se inserta su obra en la narrativa cubana. Las novelas estudiadas son: La gema de Cubagua, Fugas y Deja que se muera España. Se comparan estas tres novelas con obras de otros escritores canónicos cubanos que han escrito su obra en el exterior de Cuba, como también lo está haciendo Navarrete. Con La gema de Cubagua se utiliza la óptica de los conceptos de carnavalización y parodia del crítico soviético Bajtín; así como también el choteo según el filósofo y …

Succession In Native Groundwater Microbial Communities In Response To Effluent Wastewater, Chelsea M. Dinon

USF Tampa Graduate Theses and Dissertations

Aquifer storage and recovery techniques are used globally to mitigate increasing demands for groundwater. Microcosms were used to evaluate the effect of wastewater effluent that may be used for aquifer storage and recovery injection on native aquifer microbial communities. Sulfur Springs water was used as the source of aquifer water. The microcosms were 100% spring water, 100% unchlorinated filtered wastewater effluent, or one of three mixtures of unchlorinated filtered wastewater effluent and spring water. The mixture microcosms were 50%, 30%, and 10% unchlorinated filtered wastewater effluent with the balance as spring water. The effluent water was UV treated prior to …

Honokiol Loaded Mpeg-Pcl Micelles For The Treatment Of Age-Related Macular Degeneration, Amna Shahid

USF Tampa Graduate Theses and Dissertations

Age related Macular Degeneration (AMD), a multi-factorial age-related retinal hypoxic disorder resulting in irreversible loss of vision is the foremost cause of blindness in United States. Current treatment strategies used to treat disease involve multiple intraocular injections of Anti – VEGF agents into the vitreous of eye. In addition to the issues of drug localization and targeted delivery, the need to frequently inject drug into the eye raise patient compliance issues. These challenges call for sustained drug delivery system. In this study, a sustained drug delivery system was prepared by loading an anti- HIF agent, honokiol into MPEG-PCL polymer. These …

The Quantification Of Heavy Metals In Infant Formulas Offered By The Florida Wic Program, Naya Martin

USF Tampa Graduate Theses and Dissertations

Many infants rely on the WIC program to provide their main source of sustenance in theform of infant formula. In 2016, about 86% of the eligible infant population participated in this program. Infants in Florida account for about 30% of participation in the Southeast. For this reason, it is necessary to ensure that the formulas offered by this program provide the best opportunity for an optimal health outcome.

Exposure to heavy metals can have detrimental effects on the development of infants and children. Arsenic, lead, and cadmium specifically have been commonly found in certain types of infant formulas. Significant exposures …

La Tierra Que Pisamos: El Tercer Espacio En La Narrativa De Jesús Carrasco, Christina Ahmed

USF Tampa Graduate Theses and Dissertations

Este estudio explora las diferentes manifestaciones del tercer espacio en la novela de Jesús Carrasco, La tierra que pisamos (2016), para mostrar cómo la narrativa es capaz de generar empatía en el lector. Un ejemplo de Historia alternativa, la novela narra la colonización de España por un Imperio imperialista. Los eventos se sitúan en un pueblo en Extremadura donde un indígena llamado Leva aparece en la finca de Eva, la esposa de un militar del Imperio. La interacción entre los dos personajes principales provoca un cambio en la mentalidad de Eva, ya que se hace consciente de cómo su país …

Control Of A Human Arm Robotic Unit Using Augmented Reality And Optimized Kinematics, Carlo Canezo

USF Tampa Graduate Theses and Dissertations

There are more than 350000 amputees in the US who suffer loss of functionality in their daily living activities, and roughly 100000 of them are upper arm amputees. Many of these amputees use prostheses to compensate part of their lost arm function, including power prostheses. Research on 6-7 degree of freedom powered prostheses is still relatively new, and most commercially available powered prostheses are typically limited to 1 to 3 degrees of freedom. Due to the myriad of possible options for various powered protheses from different manufacturers, each configuration is governed by a distinct control scheme typically specific to the …

Motivation To Volunteer, Lendi N. Joy

USF Tampa Graduate Theses and Dissertations

The rate of volunteering in the U.S. remains low in spite of the many positive benefits that volunteering yields. Prior research has suggested various theories to help explain motivation to volunteer. Nonetheless, none of the theories have been both comprehensive and specific enough to allow for their practical application in recruitment efforts or campaigns to increase motivations to volunteer. The purpose of the current study was to test a comprehensive model of volunteer motivation by integrating the Volunteering Functions Inventory into the Theory of Planned Behavior as behavioral and normative beliefs that influence attitudes and subjective norms. The hypothesized model …

Convergence Of Auditory Nerve Fibers Onto Globular Bushy Cells, Matthew Kersting

USF Tampa Graduate Theses and Dissertations

Globular bushy cells (GBCs) of the cochlear nucleus are specialized neurons that encode the temporal features of sound. Multiple auditory nerve inputs are known to synapse onto a single GBC, but the exact number and sizes of these inputs have not been systematically investigated in adult mice. To gain a high-resolution and unbiased look at the auditory inputs contacting GBCs, our lab utilized Serial Block-Face Scanning Electron Microscopy. Specifically, 21 GBCs and all their large inputs were reconstructed at nanometer resolution. To produce the most precise results, we applied careful attention to the reconstruction and implemented cutting-edge meshing algorithms. We …