Digital Commons Network^™

Techno-Economic Analysis Of Polyethylene Terephthalate Bio-Upcycling, Benard Nyawanga

USF Tampa Graduate Theses and Dissertations

Plastics have been used in almost every household appliance and various industrial applications for more than one hundred years. Plastic is among the most used materials in manufacturing worldwide. Plastic is cheap, light, durable, flexible, non-toxic, etc., and these properties make it suitable to use in manufacturing many types of appliances and materials. There has been an excessive reliance on plastic, leading to mass production. Despite being overly produced, there has been very little effort to recycle plastic, leading to massive accumulation of this environment. Plastic wastes are found in large patches in the world's oceans, waterways, and land. In …

Intersections Of Race And Place In Short Fiction By New Orleans Gens De Couleur Libres, Adrienne D. Vivian

USF Tampa Graduate Theses and Dissertations

Although New Orleans joined the United States following the Louisiana Purchase, the city’s French colonial period continued to influence New Orleanians. The lives and writing of nineteenth century New Orleans gens de couleur libres, free people of color, document continued exchanges with France and the Caribbean despite the city’s increasing Americanization. Drawing from Westphal’s theoretical work on geocriticism, Intersections of Race and Place in Short Fiction by New Orleans Gens de Couleur Libres locates sites of transgressivity and their representations in writers Michel Séligny, Adolphe Duhart, and Victor Séjour’s French language short stories. Chapter One examines New Orleans’s historical and …

Investigating The Novel Role Of Dextromethorphan In Mitigating Oxidative Stress Induced Changes In Retinal Pigment Epithelial Cells (Rpe), Zeinab A. Mohamed

USF Tampa Graduate Theses and Dissertations

Oxidative stress contributes to the degeneration of retinal pigment epithelial (RPE) cells and photoreceptors that lead to the dry form of age-related macular degeneration. Activation of N-Methyl-D-aspartate (NMDA) receptors contributes to the increase of free radicals that induce oxidative stress-induced changes. Our study investigates if Dextromethorphan, an NMDA receptor antagonist, reverses oxidative stress-induced changes in RPE. We used Paraquat and H2O2 as oxidative stress-inducing agents to induce oxidative damage in human ARPE-19 cells. To illustrate the change in cell viability and measure the apoptosis of ARPE-19 cells from oxidative damage, we performed MTT assay and Annexin V-DAPI flow cytometry analysis. …

The Mass Is The Medium: Marshall Mcluhan And Roman Catholic Liturgical Change, Ashil D. Manohar

USF Tampa Graduate Theses and Dissertations

The liturgical changes of Vatican II sought to bring a period of renewal to the Catholic laity by encouraging active participation and making the Mass more simple to understand. However, the time after the Council was characterized by confusion and disengagement by the laity. I will be discussing three significant changes to the liturgy at this time, The change from Latin to the vernacular, the change in the orientation of the altar and priest, and the change in acoustics, sound, and music. I will be examining all of these changes through the media theory of Marshall McLuhan, famous for coining …

"Duck Wars": Examining The Narrative Construction Of A "Problem" Species, Jenna A. Bateman

USF Tampa Graduate Theses and Dissertations

The problematization of non-human animals occurs through a process of claimsmaking that constructs certain species as “problems”. My thesis examines the news narrative constructions of the Muscovy duck in Florida and Texas. I use a narrative analysis to examine the themes through which news narratives make claims in their construction of the “duck wars” in Florida and Texas. In the “duck wars”, the problematization of the Muscovy occurs through a set of claims made about the species by the reporters and residents in Florida and Texas neighborhoods. There are also sets of claims about other groups associated with the species, …

Novel Educational Material For Patients With A Variant Of Uncertain Significance (Vus) In A Cancer Risk Gene, Meghan E. Kelley

USF Tampa Graduate Theses and Dissertations

The number of individuals being tested for hereditary cancer syndromes has greatly increased in the last several years and many people receive Variants of Uncertain Significance (VUS) as a test result. Although VUS results should not guide medical management, patients and even some healthcare providers continue to use a VUS to alter or receive unnecessary medical care.

We conducted a needs assessment via literature review and analyzed VUS patient interviews from a previous study with the goal of identifying various themes that could help determine content, layout, and messaging to incorporate into online educational materials. The needs assessment found few …

Four Hollywood Film Adaptations Of Little Women: Identifying Female Subjectivity In Characters, Plots, And Authorship, Haiyu Wang

USF Tampa Graduate Theses and Dissertations

My thesis examines the expressions of the limits and possibilities of female subjectivity in the four Hollywood film adaptations of Little Women, filmed in 1933, 1949, 1994, and 2019 respectively, by combining textual and audience analysis with adaptation theory, feminist film theory, and feminist adaptation theory. In exploring the disparate femininity of female characters, the different representations of plots, and the authorship and changes of structure, I argue that the 1933 and 1949 versions present evidently conservative and patriarchal characteristics in the text, but their female subjectivity can be obtained through the complexity of emotion of the female audience …

Analyses Of Woodland Check-Stamped Ceramics In Northwest Florida, John D. Blackburn

USF Tampa Graduate Theses and Dissertations

This research compares prehistoric check-stamped ceramics from two northwest Florida sites, Sunstroke (8Li217) and Roy Whitfield (8Gu52), to investigate change through time in pottery manufacture of this ubiquitous type from the Early to Late Woodland Periods, about 100-1000 C.E. These sites are about 60 km apart and connected by waterways in the middle and lower Apalachicola valley drainage system. The Roy Whitfield site is considered Early Woodland based on other diagnostic pottery types, and the Sunstroke site has been radiocarbon-dated to the Late Woodland Period. Check-stamping is a common form of ceramic surface treatment across the Woodland Period and beyond, …

The Little Things That Count: Mapping Diversity Of West Florida’S Shelf, Nicole L. Seiden

USF Tampa Graduate Theses and Dissertations

Biodiversity is essential to the ecosystem functions responsible for generating the multi-billion-dollar industries that the US relies on for food, storm mitigation, tourism and recreation, but is facing increasing natural and anthropogenic threats. High-resolution diversity data covering large spatial areas would enhance current ecology knowledge and better address conservation concerns in the Gulf of Mexico. However, acquiring and handling data of this nature is resource intensive and has not yet been established. In this study, high spatial resolution data was collected from gastropod living and death assemblages collected over 10 years from the West Florida Shelf (WFS). A multivariate regression …

Comparison Of The Effectiveness Of Disinfectant-Impregnated Wipes Versus Detergent Wipes For Surface Decontamination, Jacob Amadin

USF Tampa Graduate Theses and Dissertations

Over time, there is an increased need to adopt more advanced and effective ways of disinfection. The emergence of the coronavirus-2019 (COVID-19) pandemic reminded the world on the importance of disinfection. Disinfection has been a routine in the healthcare setting. However, with the current pandemic problem, it is more prominently and persistently done in the domestic setting and other business settings beyond healthcare. As such, the use of most disinfectants and detergent wipes to achieve a near sanitized environment has become a common practice around the globe. This research presents the efficacy of disinfectant impregnated versus detergents wipes through comparison. …

Drug Candidates Targeting Hsp90/Cdc37 For The Treatment Of Neurodegenerative Disease, Ryan Martin

USF Tampa Graduate Theses and Dissertations

Neurodegenerative disease refers to the death of specific populations of neurons that canresult in dementia, loss of voluntary muscle motility, and other abnormalities. Two major neurodegenerative diseases are Alzheimer’s disease (AD) and Parkinson’s disease (PD), which are linked to abnormalities in Tau and a-synuclein proteins, respectively. Currently, there is no cure for these diseases. Our group and others have shown that chaperone proteins play a critical role in the processing of Tau and a-synuclein. The inhibition of the Hsp90/Cdc37 chaperone complex is shown to affect the levels of these two proteins. Hence, we hypothesized that a drug molecule, conglobatin (Cb), …

This Is It: Latina/X Representation On One Day At A Time, Camille Ruiz Mangual

USF Tampa Graduate Theses and Dissertations

This thesis examines the tensions between contemporary Latina/x representations and problematic tropes in the sitcom, One Day at a Time (2017-2020) [ODAAT]. ODAAT centers of Elena, Penelope, and Lydia, three generations of a Latina/x family. Many entertainment reviewers and fans praised the series for its progressive and nuanced portrayals of Latina/x characters. However, I argue that while ODAAT depicts Latina/x characters that transcend some United States mainstream media tropes about Latinas/xs, the series also relies on conventional markers of Latina/x identity as tools with which to communicate progressive messages around identity. I expand upon scholarship in Latina/o media …

Nest Site Choice As A Potential Climate Response In The Gopher Tortoise (Gopherus Polyphemus), A Threatened Species, Meghan Craft

USF Tampa Graduate Theses and Dissertations

Climate warming is expected to continue in coming decades, posing a threat to species worldwide. While some species may be able to shift their ranges in the pursuit of cooler temperatures, a large portion of species may have to find ways to adapt to warmer conditions in situ. Oviparous reptiles may be able to buffer against rising temperatures by altering nest site choice. In this study, I quantified nest site choice and its thermal consequences in a population of the gopher tortoise (Gopherus polyphemus) in southwestern Florida. I found marked variation in the openness of selected nest sites, …

Quantifying The Gametogenic Cycle And Reproductive Efforts In The Eastern Oyster (Crassostrea Virginica) In Florida Estuaries, Nicole Patrice Maloney

USF Tampa Graduate Theses and Dissertations

Oyster populations from six estuaries in Florida were sampled monthly from January to December of 2018 for reproduction and health analysis. Sites were located in Apalachicola Bay, Caloosahatchee River estuary, Lake Worth lagoon, Loxahatchee River estuary, St. Lucie estuary, and Tampa Bay. Oysters monitored for analysis in South Florida (Loxahatchee River estuary, St. Lucie estuary, and Caloosahatchee River estuary) were chosen as they are a part of a monitoring component of the Comprehensive Everglades Restoration Plan. These estuaries are highly influenced by freshwater input from Lake Okeechobee and other river basins. Like the South Florida sites Apalachicola Bay is also …

¿De Dónde Eres?: Negotiating Identity As Third Culture Kids, Sophia Margulies

USF Tampa Graduate Theses and Dissertations

Sparked by David C. Pollock's (1988) concept of "third culture kids" (children of government officials and military personnel), this thesis uses autoethnography to examine "white-passing" Latinx identity and gender passing as it relates to individuals who identify as transgender to understand what it means to "pass" within these communication contexts. I situate the study at the intersections of queer, trans, and Latinx theories. Ultimately, I argue that the communicative and identity practices inherent to the liminal spaces in which third culture kids perform create the conditions for performances as transnational subjects. What the contexts of place and home are like, …

Glutathione-S-Transferase Mu 1 (Gstm1) Gene Augmentation Attenuated Oxidative Stress Induced Changes In Retinal Pigment Epithelium Cells (Rpe), Riddhi Sandeep Vichare

USF Tampa Graduate Theses and Dissertations

Purpose: Loss of Retinal Pigment Epithelial (RPE) cells and photoreceptors leads to Age-related macular degeneration (AMD), one of the leading causes of blindness in the elderly worldwide. Oxidative stress-mediated damage is one of the factors that impair the survival of RPE and photoreceptors in dry-AMD. We sought to determine the role of human Glutathione S-Transferase Mu-1 (GSTM1) in protecting RPE cells from oxidative stress-induced changes.

Method: We cloned human GSTM1 cDNA with P2A tag into a lenti-plasmid vector and later made a lentivector to express GSTM1. After that, we infected human retinal pigment epithelial cells (ARPE-19), screened stable ARPE-19 cells …

Seasonality, Labor Organization, And Monumental Constructions: An Otolith Study From Florida’S Crystal River Site (8ci1) And Roberts Island Shell Mound Complex (8ci40 And 41), Elizabeth Anne Southard

USF Tampa Graduate Theses and Dissertations

In recent decades, archaeological research has provided evidence that some mounds in the southeastern United States were constructed in short episodes. A large work force would have been required to accomplish these monumental projects. Shell mounds, in particular, provide an opportune type of architecture to investigate whether seasonal aggregations of laborers gathered at sites to engage in large-scale work projects because these mounds are constructed of aquatic resources that leave signatures for what time of the year they were caught or harvested. This study investigates whether the residents of the Crystal River site (8CI1) and Roberts Island (8CI40 and 41) …

Exploring The Use Of Neural Transformers For Psycholinguistics, Antonio Laverghetta Jr.

USF Tampa Graduate Theses and Dissertations

Deep learning has the potential to help solve numerous problems in cognitive science andeducation, by providing us a way to model the cognitive profiles of individual people. If this were possible, it would allow us to design targeted tests and suggest specific remediation based on each individual’s needs. On the flip side, employing techniques from psychology can give us insight into the underlying skillsets neural networks have acquired during training, addressing the interpretability concern. This thesis explores these ideas in the context of transformer language models, which have achieved state-of-the-art results on virtually every natural language processing (NLP) task. First, …

Effect Of Temperature On Growth And Gene Expression In The Marine Diatom, Thalassiosira Pseudonana, John M. Foster

USF Tampa Graduate Theses and Dissertations

Diatoms are unicellular, eukaryotic, photosynthetic algae responsible for the production of 20% of our atmospheric oxygen. Diatoms contribute to several important biogeochemical functions. Diatoms contribute to the carbon cycle, sequestering carbon and forming oxygen as a product of photosynthesis. Moreover, by converting abiotic forms of energy such as sunlight into organic compounds (i.e., sugars, starches and lipids), primary producers including diatoms, plants, and phytoplankton feed organisms at higher trophic levels. Consequently, changes in temperature, light intensity, nutrients, salinity and other stress factors that affect primary producers can generate a potentially catastrophic ripple effect at higher trophic levels In this study …

Politics Vs. The Environment: The Spatial Distributions Of Mississippian Mound Centers In Tampa Bay, Adam J. Sax

USF Tampa Graduate Theses and Dissertations

The Safety Harbor culture that resided in West-Central Florida during the Mississippian period (~1000-1500 CE) was distant from the Mississippian heartland but built similar platform mound complexes and exhibited social hierarchies despite practicing an estuarine lifestyle that likely did not rely on extensive agriculture. To determine whether this coastal culture exhibited similar spatial patterns of platform mound centers to traditional inland cultures, GIS spatial analyses including distance matrices, density analyses, and least cost analyses (LCA) were performed within the Safety Harbor geographical nexus of Tampa Bay. The results were able to detect temporal changes in settlement patterns and estimate the …

Our Story, Our Homeland, Our Legacy: Settlement Patterns Of The Geechee At Sapelo Island Georgia, From 1860 To 1950, Colette D. Witcher

USF Tampa Graduate Theses and Dissertations

The Gullah Geechee are descendants of enslaved Africans who worked on the island coastal plantations of Georgia, North and South Carolina, and Northern Florida. The Gullah Geechee are known for having retained African cultural connections and practices in the United States through intergenerational knowledge transmission. On Sapelo Island, Georgia, during their 250 years on the island a least 15 historic communities have been acknowledged as representative of their distinct presence on the island. Hog Hammock which was established in 1878, is the last intact Gullah Geechee owned community, due to forced abandonment of the other communities during the early 1950s. …

The Impact Of Bahiagrass (Paspalum Notatum) On Florida Sand Skink (Plestiodon Reynoldsi) Movements, Chandler A. R. Eaglestone

USF Tampa Graduate Theses and Dissertations

The Florida Sand Skink (Plestiodon reynoldsi) is a federally threatened lizard precinctive to scrub habitat, a pyrogenic habitat that experiences fires infrequently. Plestiodon reynoldsi spends most of its time right under the sand surface, moving, or “sand swimming,” through the granular substrate found in Florida’s central ridges. While the distribution of this species has been greatly reduced, agricultural development of P. reynoldsi habitat has led to potentially suitable fragmented scrub occurring near bahiagrass (Paspalum notatum) pastures. Bahiagrass is widely used as a pasture grass in sandy soils of the southeast United States, because of its deep …

Social Cues In Animated Pedagogical Agents For Second Language Learners: The Application Of The Embodiment Principle In Video Design, Sahar M. Alyahya

USF Tampa Graduate Theses and Dissertations

This study investigated the involvement of the Social Agency Theory within a multimedia learning environment to improve English language proficiency. The primary aim of this study is to find the effects of designing language learning instructional videos following the embodiment principle on language learners’ motivation, cognitive load and performance in production of the target language. According to Social Agency Theory, when multimedia learning includes social cues like an on-screen agent with humanlike features (e.g., hand gestures, body movements, eye contact, and facial expressions), the quality of learning will be increased. To examine the effect of the embodiment principle, the study …

Evidences Of Historical Thinking In Dialogic Discussions, Jennifer L. Johnson

USF Tampa Graduate Theses and Dissertations

Research continues to find discussions in history classrooms to be rather short in length and simplistic in nature, despite the higher aims of historical and critical thinking. The purpose of this qualitative study was to explore the phenomenon of historical thinking and dialogic discussions in the secondary history classroom. Its goal was to better understand the nature of both, as well as to see if whole-class discussions might serve as a useful means in cultivating historical thinking in students. Given the need for more research in these areas, this study was designed with the hopes of adding to the literature …

Plastic Pollution In Urban Rivers: Spatial And Temporal Patterns Of Plastic Release And Transport, Charlotte Juliane Haberstroh

USF Tampa Graduate Theses and Dissertations

This dissertation investigates plastic release and transport in urban rivers with a focus on the driving spatial and temporal factors. Plastic is a key pollutant of this century that affects humans and the environment around the world. Rivers are a focal point of release and management, as they concentrate and accumulate plastics from urban watersheds and convey them to the world’s oceans.

This research assessed cross-sectional, longitudinal, and seasonal patterns of plastics in rivers, and evaluated the role of urban pollution and watershed hydrology. A detailed background is provided in the literature review. This work is built on two case …

Bone Microenvironmental Control Of Skeletal Malignancy, Chen Hao Lo

USF Tampa Graduate Theses and Dissertations

Bone is a common site of metastasis for many solid malignancies. Bone-metastatic cancers pose a significant clinical problem worldwide and is among the main causes for cancer patient morbidity and mortality. Patients with advanced bone-metastatic diseases often present with either osteolytic or osteogenic bone diseases as their cancers progress. These bone pathologies are products of the cancer co-opting the local bone remodeling stroma to yield important growth nutrients and factors. Unfortunately, skeletal metastases remain incurable and are fatal. Identifying and understanding the causal multicellular and molecular interactions underlying skeletal malignancies can yield crucial ideas for targeting and inhibiting disease progression. …

Problematizing Florida’S Extended Reading Time Policy: A Critical Investigation Of Place, Demographics, And Curricula, Carrie L. Gentner

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to investigate Florida’s extended reading time (ERT) policy as enacted during the 2016-2017 school year. This policy requires elementary schools ranking among the 300 lowest performing in the state, as measured by state assessments, to provide an additional hour of reading instruction beyond the regular school day. Extending upon the work of a pilot study suggesting an overrepresentation of minoritized students within low 300 schools, this study presents the geographic, demographic, and socioeconomic distributions of the ERT mandate for a criterion sample of seven districts. Further, the pilot study indicated an underrepresentation of minoritized …

Resilience And Burnout In Second- And Third-Year Medical Students, Chad E. Whistle

USF Tampa Graduate Theses and Dissertations

Between their second- and third-years of medical school, students must pass the United States Medical Licensing (USMLE) Step 1 exam. This high-stakes exam is critical to the overall success of medical students; the score has been a determining factor for the student’s residency training and specialty choice. Because medical students are faced with the burden of studying and concept mastery of content for USMLE Step 1, concurrent to ongoing coursework in the medical school curriculum, students may develop symptoms of burnout and be ill-prepared to remain resilient. This study investigated the extent of the relationship between burnout and resilience in …

Biosynthetic Gene Clusters, Microbiomes, And Secondary Metabolites In Cold Water Marine Organisms, Nicole Elizabeth Avalon

USF Tampa Graduate Theses and Dissertations

Microorganisms and invertebrate animals from cold water marine environments, such as in Antarctica and in Ireland’s deep sea, are a rich source of secondary metabolites. In this dissertation, research was centered around secondary metabolism and natural product biosynthesis. The projects include the delineation of biosynthetic gene clusters hypothesized to be responsible for the biosynthesis of the palmerolides, stratification of the microbiome of the Antarctic ascidian Synoicum adareanum, genomic and peptidomic analysis of a host-associated Antarctic Pseudovibrio sp., and isolation and characterization of secondary metabolites from the Irish deep-sea coral Drifa sp.

Palmerolide A, the principle secondary metabolite associated with S. …

Cognition In Health And Disease: Two Mouse Model Studies, Heather Lynn Mahoney

USF Tampa Graduate Theses and Dissertations

Cognitive and circadian dysfunction are common aspects of neuropsychiatric disease that are often overlooked in disease models and treatment strategies. In this work, we first evaluated the potential for cognitive enhancement in healthy adult mice by targeting a kinase involved in regulation of the molecular circadian clock. We then developed and characterized a model of neuropsychiatric disease that could present a target for rescuing circadian and cognitive deficits using this treatment.

Time-of-day effects have been noted in a wide variety of cognitive behavioral tests, and perturbation of circadian rhythms impairs hippocampus-dependent learning and memory. We first asked whether casein kinase …