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Mercury In The Environment: Field Studies From Tampa, Bolivia, And Guyana, Joniqua A'Ja Howard

USF Tampa Graduate Theses and Dissertations

Tampa (US), Guyana (SA), and Bolivia (SA), are geographically, socially, economically, and politically unique which make them ideal sites to study issues of mercury and sustainability. Mercury’s innate ability to bioaccumulate and biomagnify in aquatic and terrestrial ecosystems poses a severe threat to both human and environmental health. The most vulnerable populations affected by mercury consumption include coastal communities, children, women of child-bearing age, the indigenous poor and persons with high environmental/occupational exposure factors. Communities in the regions of Florida, Bolivia, and Guyana whose diets are high in fish and are environmentally/occupationally exposed to mercury may be at a higher …

A Potential Tumor Suppressive Role Of Sirt1 In Cancer, Neha Kabra

USF Tampa Graduate Theses and Dissertations

The NAD-dependent deacetylase SIRT1 regulates several factors involved in stress response and cell survival but its function in cancer is largely unknown. Research suggests that SIRT1 influences several transcription factors and molecules that are important components of pathways often deregulated in cancer. Our experiments have shown that SIRT1 knock down by short hairpin RNA accelerates tumor xenograft formation by HCT116 colon cancer cells, whereas SIRT1 overexpression inhibits tumor formation. We have also found that, pharmacological inhibition of SIRT1 stimulates cell proliferation under conditions of growth factor deprivation suggesting a tumor suppressive function of SIRT1. Paradoxically, SIRT1 inhibition sensitizes the same …

The Life History And Energy Budget Of Hippocampus Erectus In Tampa Bay, Florida, Nicole M. Dunham

USF Tampa Graduate Theses and Dissertations

Seahorses, genus Hippocampus erectus, are subject to large and continuously-growing international trade. Concerns with the effects of trade on seahorse population worldwide have led to their international protection by the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). In order to manage seahorse populations, we first need to understand their basic biology. The purpose of this project was to establish an energy budget for Hippocampus erectus from Tampa Bay, Florida. A total of 108 specimens were collected throughout Tampa Bay from 2004 to 2007 using 21.3 m offshore seines and 6.1 m otter trawls. …

Passive Acoustic Studies Of Estuarine Fish Populations Of Southwest Florida, James Vincent Locascio

USF Tampa Graduate Theses and Dissertations

Recordings of fish sound production were made in Charlotte Harbor, Florida using Long Term Acoustic Recording Systems (LARS) programmed to record 10 seconds of sound every 10 minutes. Results demonstrated a strong circadian pattern in fish sound production that occurred within a few hours of dusk each evening. Sound production lasted on average 8.7 hrs each evening during the peak spawning season. LARS were deployed when Hurricane Charley crossed Charlotte Harbor in August, 2004. The hurricane did not inhibit nightly chorusing events of spawning fish. Rather, sound levels produced by spawning fish on the night of and 3 days after …

The Distribution And Population Dynamics Of The Golden Mouse (Ochrotomys Nuttalli) At Its Southern Range Periphery, Sarah A. Smiley

USF Tampa Graduate Theses and Dissertations

This research assesses the status of the golden mouse (Ochrotomys nuttalli) in Florida by taking a multi-pronged approach. Geographic Information Systems (GIS) was used to understand the distribution of habitats and occurrence records for this species within the state. Presence-absence trapping occurred at 13 study sites to determine if historic southern periphery populations were still occupied, gauge if more central populations were being maintained, and document golden mice in previously unrecorded areas. In addition, surveys for O. nuttalli took place at regular intervals at the USF Ecological Research Area to understand how populations of this species fluctuate over …

Assessing The Effectiveness Of The Roaring Branch Bmp Retrofit Using Macroinvertebrate Bioassessment, James L. Banning

USF Tampa Graduate Theses and Dissertations

Using benthic macroinvertebrates to measure stream health has been widely used and accepted around the world. Macroinvertebrates are resident monitors of chronic impairment in a stream since they are relatively sessile and most commonly respond to disturbance by drift but can recolonize a restored stream reach very quickly. This study tested the effectiveness of macroinvertebrate metrics developed through the Rapid Bioassessment Protocol (RBP) to detect changes in stream integrity as the result of placement of a best management practice (BMP), installed on a tributary of Roaring Branch, located in Columbus, Georgia. The BMP was designed to attenuate flow to reduce …

Where Christ Dies Daily: Performances Of Faith At Orlando‘S Holy Land Experience, Sara B. Dykins Callahan

USF Tampa Graduate Theses and Dissertations

This manuscript focuses on performances of place and faith inside the Holy Land Experience (HLE), an edutainment complex nestled in the fantasy nexus of Orlando, Florida. A self-proclaimed living-history museum, the HLE includes animatronic Bible characters and musical dramas. The HLE enacts and embodies evangelical narratives of Christianity and Christian faith, and visitors to the park are asked to join the performances, blurring the distinctions between spectators and professional actors. I argue that visitors' performances of faith invest the space of the HLE with sacredness, while the location and design of the HLE infuses the space with elements of the …

On The Involvement Of The Low-Density Lipoprotein Receptor In The Pathogenesis And Progression Of Alzheimer’S Disease, Jose Francisco Abisambra Socarras

USF Tampa Graduate Theses and Dissertations

Alzheimer's disease (AD) is the most prevalent form of age-associated dementia. Cholesterol dysregulation is linked with AD onset. Besides age, the most important risk factor associated with AD is the inheritance of the epsilon-4 allele of apolipoprotein E, a cholesterol transporter. In addition, while hypercholesterolemia has been shown to be an independent risk factor for AD, the nature of the cholesterol-AD link is still not clear. This gap in our understanding is partly due to a lack of knowledge about cholesterol metabolism in the central nervous system (CNS).

The low-density lipoprotein receptor (LDLR) is the main receptor of apoE and …

Active Surveillance And Incidence Rate Of Dengue Infection In A Cohort Of High Risk Population In Maracay, Venezuela., Carlos Espino

USF Tampa Graduate Theses and Dissertations

In the absence of an effective vaccine, vector control and surveillance of dengue fever (DF) and dengue hemorrhagic fever (DHF) are the most important strategies currently used to reduce the impact of these diseases in affected population. The objectives of this study were to estimate the incidence of symptomatic and asymptomatic dengue cases, the prevalence of antidengue antibodies, and to evaluate the laboratory and clinical aspects related to an active surveillance of dengue cases. In this study, active surveillance was incorporated as a part of the study design. At total of 3,255 people from four high risk neighborhoods were followed …

Developing The Nomological Network Of Perceived Corporate Affinity For Technology: A Three Essay Dissertation, David Earl Fleming

USF Tampa Graduate Theses and Dissertations

Technology is changing the face of both the sales and service domains. Honebein and Cammarano (2006) note that properly implemented self-service technologies serve dual purposes of decreasing firm overhead costs, while simultaneously engaging the customer in a way encourages the co-create of value for both parties. To get these benefits stakeholders must be willing to adopt and use the technologies that are available.

Traditionally, this has lead to the research question "How do firms do this?" However, according to a recent article by Woodall, Colby and Parasuraman (2007), consumers are now demanding more technology-based options and becoming more technologically savvy. …

The Sleeping Giant: Revealing The Potential Energy Of Abandoned Industry Through Adaptive Transformation, Wesley A. Bradley

USF Tampa Graduate Theses and Dissertations

With the downfall of industry, cityscapes have become speckled with post-industrial building fragments. Skeletons, left as the evidence of a past generation are waiting patiently for their chance to reemerge as active elements. Furthermore, they are ever-present but remain out of touch within the thriving urban organism that has engulfed their surroundings. These condemned giants, rich in history, location, aesthetics, and vigor sleep underneath the growth. Their post-industrial urban sites are generally discarded and forgotten. On these sites the fragments of the massive and extraordinary crumble. These large industrial buildings have become a burden to their communities. Not realizing what …

The Effect Of Stress On Hedonic Capacity In Generalized Anxiety Disorder: A Prospective Experimental Study Of One Potential Pathway To Depression, Bethany H. Morris

USF Tampa Graduate Theses and Dissertations

A growing body of work links psychopathology to changes in hedonic capacity following stressors. This was the first experimental study of the effects of stress on hedonic capacity in an analog generalized anxiety disorder (GAD) sample (a high worry group). Specifically, we utilized an experimental manipulation of stress and a behavioral index of anhedonia to test the hypothesis that individuals with GAD, who are at higher risk for developing depression symptoms, exhibit greater stress-related deficits in hedonic capacity than do nonanxious controls. Further, this study assessed whether stress-induced hedonic deficits predicted future depression. Controls exhibited the expected reward learning pattern …

Aquatecture: Architectural Adaptation To Rising Sea Levels, Erica Williams

USF Tampa Graduate Theses and Dissertations

Our world is drastically changing. Temperatures are rising, skies over cities are blanketed with smoke, and melting glaciers are raising sea levels at alarming rates. Although the destruction we face is already threatening the quality of life for billions around the world, it could just be the beginning. What is projected to come in the future could be catastrophic.

It is crucial to realize that climate change is already happening. One of the main concerns relating to climate change is that as the polar ice caps continue to melt, rising water will invade our coastal cities around the world. In …

The Politics Of Space And Place In Virginia Woolf’S The Years, Three Guineas And The Pargiters, Ángel Luis Jiménez

USF Tampa Graduate Theses and Dissertations

A critique of the social construction of space was fundamental to Virginia Woolf's overall feminist project of decentering patriarchal and imperial values. In A Room of One's Own (1929) Woolf famously emphasized that financial independence and a private space were vital to female creativity. But Woolf was concerned with the politics of space throughout her writing, an aspect of her thought that has not been widely addressed. My thesis examines Woolf's ongoing preoccupation with spatiality in two closely related works of her late career, The Years (1937) and Three Guineas (1938). In these texts, Woolf interrogates the cultural construction of …

Non-Calanoid Copepods At The Bermuda Atlantic Time-Series Study (Bats) Station: Community Structure And Ecology, 1995-1999, Hussain Ali Al-Mutairi

USF Tampa Graduate Theses and Dissertations

Zooplankton were sampled on a monthly basis at the Bermuda Atlantic Time-series Study (BATS) site from January 1995 to December 1999. Samples were collected using a 1-m², 200 µm mesh net. The net sampled the water column in an oblique manner from the surface to a mean depth of 200 m. One day and one night tow from each cruise was examined microscopically to determine the community structure of the non-calanoid copepods. In addition, a three year set of nighttime samples were examined taken by 0.5-m², 20 and 35 µm mesh nets (1995-1996 and 1997, respectively) towed obliquely to 150 …

Frozen Assets: Science, Natural Philosophy, And The Quest For Arctic Gold, Justin V. Castells

USF Tampa Graduate Theses and Dissertations

This paper looks at the emerging conflict between natural philosophy and empirical science in the late sixteenth century by examining the events surrounding the supposed discovery of gold in northern North America by Martin Frobisher in 1577. The discovery of gold in a region thought incapable of producing the metal, and the subsequent assays of ore mined from that region served as a catalyst for conflict between different understandings of the natural world. Proponents of natural philosophy and empirical science each used their theoretical tools to prove or disprove the value of the ore, reflecting the larger discussions taking place …

Defining A Community: Controlling Nuisance In Late-Medieval London, Natalie J. Ciecieznski

USF Tampa Graduate Theses and Dissertations

Using municipal sources from late medieval London, this study examines nuisance as a sub-topic of social regulation. In addition to defining nuisance, it analyzes who controlled nuisance and how it was controlled from the late thirteenth through the early fifteenth centuries. During this period, nuisance comprised building and boundary disputes between neighbors, such as conveying rainwater onto a neighboring property instead of to the street; environmental issues, such as blocking passageways with rubbish and not properly disposing of waste; certain groups of people and places, such as vagrants and brothels; and certain forms of speech, such as insults and threats. …

Workin’ From Cain To Cain’T: Challenges Within Florida’S Gulf Coast Oyster Industry, Diane Marie Wakeman

USF Tampa Graduate Theses and Dissertations

Oyster tongers are a cultural icon of Florida's maritime heritage and geography. Challenged for generations by the vagaries of weather, including catastrophic storms and years-long droughts, and economic uncertainties this maritime heritage is fading fast. While Florida's north and west coasts produce 90 percent of the Florida oyster harvest and ten percent of oysters consumed in the United States, the industry is at risk today for reasons including a declining demand for Florida oysters because of health concerns; water pollution; population growth and its accompanying development of condominiums, gated communities, and retail shopping centers; and declining interest in the hard …

Pegylation Of Niosomes, John A. Elliott

USF Tampa Graduate Theses and Dissertations

The research presented in this dissertation describes the creation and characterization of a novel antibody-vesicle conjugate modified with polyethylene glycol (PEG) that possesses enhanced binding to and uptake by inflammation-activated endothelial cells with improved storage stability and longer shelf-life and potential reduction in immunogenic potential compared to previous designs.

Targeted drug delivery provides an effective means of delivering therapeutic concentrations of a drug to the site or organ of action. The drug is delivered using a niosome, a vesicle with an aqueous core and a bilayer membrane composed of non-ionic surfactants and cholesterol. Antibodies that recognize specific cell antigens are …

Memory - Ness: The Collaboration Between A Library And Museum, Kelsey Doughty

USF Tampa Graduate Theses and Dissertations

Picture a historical library and a historical art museum coming together to challenge the interaction between each other to help experience, explore, and discover the past within the present. While it sounds like a good idea, it is rare to see a library and museum under one roof.

With the increasing population of tourists looking to visit places and buildings that reconnect with history, there is a higher demand for places to be able to 're-live the past' through art and literature. People enjoy visiting places where history was made and where it becomes part of a city's identity. With …

Urban-Eco-Filter: Introducing New Lungs To The City Of Beijing, Carlos Gil

USF Tampa Graduate Theses and Dissertations

It is evident that several cities in China have experienced a variety of global impacts. Beijing for instance, has become synonymous with mass-migration over the years. The immense scale of projected rural migration, the unmanageable intervention of new housing urban developments, and the high levels of air pollution in Beijing have become a global dilemma. In order to alleviate Beijing's critical ecological conditions, the introduction of "new lungs", as an urban environmental design to the city should be examined. This thesis project illustrates how urban productive landscape and bike infrastructure can be essential in urban development through the implementation of …

Towards The Development Of A Coastal Prediction System For The Tampa Bay Estuary, Heather Holm Havens

USF Tampa Graduate Theses and Dissertations

The objective of this research is to evaluate a coastal prediction system under various real world scenarios to test the efficacy of the system as a management tool in Tampa Bay. The prediction system, comprised of a three-dimensional numerical circulation model and a Lagrangian based particle tracking model, simulates oceanographic scenarios in the bay for past (hindcast), present (nowcast) and future (forecast) time frames. Instantaneous velocity output from the numerical circulation model drives the movement of particles, each representing a fraction of the total material, within the model grid cells.

This work introduces a probability calculation that allows for rapid …

The Role Of Cocrystals In Solid-State Synthesis Of Imides And The Development Of Novel Crystalline Forms Of Active Pharmaceutical Ingredients, Miranda L. Cheney

USF Tampa Graduate Theses and Dissertations

With a greater understanding of the fundamentals of crystal engineering lays the

potential for the development of a vast array of novel materials for a plethora of

applications. Addressed herein is the latent potential of the current knowledge base with

an emphasis upon cocrystallization and the desire for scientific exploration that will lead

to the development of a future generation of novel cocrystals. The focus of this

dissertation is to expand the cocrystallization knowledge base in two directions with the

utilization of cocrystals in the novel synthetic technique of cocrystal controlled solid-state

synthesis and in the development of active pharmaceutical …

Tracing Anthropogenic Wastes: Detection Of Fluorescent Optical Brighteners In A Gradient Of Natural Organic Matter Fluorescence, Laura Kellie Dixon

USF Tampa Graduate Theses and Dissertations

A dual wavelength method was developed for the field detection of optical brighteners

(OBs), fluorescent laundry additives used as indicators of anthropogenic wastes. The

method was quantitative for OBs under variable levels of fluorescent colored dissolved

organic matter (CDOM). Based on excitation at 300-400 nm and 440 and 550 nm

emission, the method assumed a constant ratio of fluorescence due to CDOM alone, even

if absolute amplitude varied. Concentrations of OBs were computed as the difference

between the observed 440 nm emission and the expected CDOM fluorescence at 440 nm,

as extrapolated from the 550 nm fluorescence and established CDOM …

Communication, Consumption And Manipulation: The Body As Language In The Films Of Jan Švankmajer, Amanda Marie Dowd

USF Tampa Graduate Theses and Dissertations

In this thesis I will analyze and discuss the work of renowned director Jan Švankmajer. Specifically, I will examine how director Jan Švankmajer’s representation of the body creates a metaphorical language. In addition, I will address what meaning can be gathered from, or made apparent through the commentary of the body’s language and discuss the significance of the socio-political implications. Prior to my discussion of Švankmajer’s work I will give a concise socio-political history of the Czech Republic from 1968-1994; this discussion will provide a framework for the subsequent analyses. In order to provide support for my argument, I will …

The Development Of A Human Polyomavirus Quantitative Pcr Assay To Assess Viral Persistence, Water Quality, And Human Health Risks, Shannon M. Mcquaig

USF Tampa Graduate Theses and Dissertations

Microbial water quality is generally assessed using fecal indictor organisms; however host-specific microbial source tracking (MST) methodologies can be employed to differentiate sources of fecal pollution. The central goal of this research was to develop and validate a QPCR assay for the quantification of two human-specific polyomaviruses (HPyVs) in environmental water samples. These viruses are prevalent worldwide and produce lifelong, asymptomatic viruria in immunocompetent individuals.

A Taqman® quantitative PCR (QPCR) assay based on the conserved T-antigen of two HPyVs (JCV and BKV) was developed and optimized (Chapter 2). HPyVs were detected in a high proportion of human-associated waste samples (e.g. …

Task Oriented Simulation And Control Of A Wheelchair Mounted Robotic Arm, Fabian Farelo

USF Tampa Graduate Theses and Dissertations

The main objective of my research is to improve the control structure for the new Wheelchair Mounted Robotic Arm (WMRA) to include new algorithms for optimized task execution; that is, making the WMRA a modular task oriented mobile manipulator. The main criterion to be optimized is the fashion in which the wheelchair approaches a final target as well as the starting and final orientation of the wheelchair. This is a novel approach in non-holonomic wheeled manipulators that will help in autonomously executing complex activities of daily living (ADL) tasks.

The WMRA is a 9 degree of freedom system, which provides …

Interrogating Virginia Woolf And The British Suffrage Movement, Gwen Trowbridge Anderson

USF Tampa Graduate Theses and Dissertations

Interrogating Virginia Woolf and the British Suffrage Movement Gwen Trowbridge Anderson ABSTRACT Much has been written about Virginia Woolf's involvement with feminism and women's rights, but there has been far less exploration about her ties to suffrage. Many of her friends and family are involved in this exploration: Vanessa Stephen Bell, Ethel Smyth, and the Pankhursts (Emmeline, Sylvia, and Cristobel). Other important figures who are relevant to Woolf's work are Sonia Delaunay, Lewis Carroll, and Edmund Spenser. Important concepts like the New Woman, the suffrage movement, feminism, and women's rights are vital to understanding Woolf's involvement with suffrage. This dissertation …

Sustainable Planning And Design For Ecotourism: Ecotecture Embraced By The Essence Of Nature On Amboro National Park, Santa Cruz-Bolivia, Claudia P. Gil

USF Tampa Graduate Theses and Dissertations

The concern for the environment and social aspects have been emphasizing in the concept of ecotourism. Tourism is the world's largest industry. "It accounts for more than 10% of total employment, 11% of global GDP, and total tourist trips are predicted to increase to 1.6 billion by 2020". As such, it has a major and increasing impact on both people and nature. The increase of travels and tourists in the world, has led to the acknowledgement of tourism as part of the emissions of greenhouse gases. Therefore, the framework of sustainable development in tourism has been developed as well as …

Hemocompatibility Assessment Of 3c-Sic For Cardiovascular Applications, Norelli Schettini

USF Tampa Graduate Theses and Dissertations

The hemocompatibility of crystalline Silicon Carbide (SiC), in its cubic form (i.e., 3C-SiC), has been evaluated and compared to Silicon (Si), the leading material in biosensing applications. Silicon carbide (SiC) is a hard, chemically robust material, very well suited for harsh environment applications, and has been suggested to have very good biocompatibility. Additionally, SiC in its amorphous form, has been used as a coating for medical implantable devices such as bone prosthetics and cardiovascular stents. However, assessment of single crystal 3C-SiC for cardiovascular applications has not been reported. In this research we have studied the interactions of single crystal 3C-SiC …