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The Emergence Of Early Visual Attention Profiles In Infants At High Risk For Autism Spectrum Disorder, Debra Reisinger

Theses and Dissertations

This dissertation is comprised of two manuscripts focused on early social and nonsocial attention in children at-risk for developing autism spectrum disorder (ASD): infants with fragile X syndrome (FXS) and infants with an older sibling diagnosed with autism (ASIBs). Each manuscript will present original research: the first will consist of a crosssectional and longitudinal examination of attention to objects in infants with FXS and infant ASIBs parsed apart by their ASD diagnostic outcomes in comparison to a group of typically developing (TD) infants, and how developmental trajectories of object attention predict later ASD symptom severity and diagnostic outcomes. The second …

(Re) Thinking The Past Through Performance: The (Re) Construction Of Militant Childhood Imaginaries In The Post-Dictatorship Of Argentina’S Cultural Production From 2003-2015, Stephanie Rubi Orozco

Theses and Dissertations

Burdened by the atrocities of Argentina’s coup d’état (1976-1982), many children of persecuted parents were forced to live as clandestine hideaways. In recent decades, adult survivors who experience the dictatorship as clandestine children have become primary protagonists, seeking to (re)construct past experiences through the creation of visual and textual productions. In attempt to (re)create their experience in hiding; such adults propose an alternative non-conventional image of a witnessing childhood under military rule as the child of prosecuted parents. For such children, life in secrecy meant having to find refuge from state officials seeking to eliminate their parents. It demanded strict …

Ground Truthing: The Politics And Culture Of Soil And Water Conservation In Iowa Agriculture, Brianna Farber

Theses and Dissertations

This dissertation explores the complex relationships between people, technologies, and ecologies involved in natural resource conservation and industrial agriculture in Iowa. Specifically I focus on the various efforts to address water pollution affected primarily by agriculture in the state. Using a theoretical framework informed by political ecology, Science and Technology Studies (STS), and posthumanist theory, I draw on thirteen months of ethnographic fieldwork to discuss what makes conservation culturally salient and practically difficult to achieve. This difficulty around conservation arises in part from the tensions between what I describe as the corn assemblage and the prairie assemblage. I identify these …

Parental Experience With Whole Exome Sequencing Reanalysis And Its Impact On The Diagnostic Odyssey, Nicole D. Lucas

Theses and Dissertations

Advances in genomic technology and an increase in the number of gene-disease associations have helped reduce the number of individuals living without a diagnosis. Whole exome sequencing (WES) analyzes the entire human exome in an attempt to determine if there is a molecular etiology for individuals who remain undiagnosed after other clinical or molecular investigations. Still, WES leaves most individuals undiagnosed, resulting in feelings of disappointment and uncertainty. Individuals who remain undiagnosed after WES can subsequently undergo WES reanalysis later due to improvements in bioinformatics, software updates, and an increase in known genedisease associations. This is the first study, to …

Dually Modified Transmembrane Proteoglycan Tβriii/Betaglycan In Cell Signaling And Cancer, Laura M. Jenkins

Theses and Dissertations

During tumor progression, cancer cells undergo a number of alterations to evade tumor inhibitory mechanisms, proliferate, invade surrounding tissues and metastasize to distant sites. These properties reflect changes in their cell signaling pathways that, in normal cells, control aberrant cell proliferation, motility, and survival. Over the past few decades, hyperactive Wnt/β-catenin signaling has been linked to the formation of multiple cancers, including malignant ovarian cancer, making identification of molecules regulating Wnt/β-catenin signaling crucial to the development of early detection methods and cancer treatment strategies. Proteoglycans, which regulate transmission of cellular signals, are implicated in the pathophysiology of diseases, including cancer, …

Climate Change And Mountaintop Removal Mining: A Maxent Assessment Of The Potential Dual Threat To West Virginia Fishes, Lindsey R F Hendrick

Theses and Dissertations

Accounts of species’ range shifts in response to climate change, most often as latitudinal shifts towards the poles or upslope shifts to higher elevations, are rapidly accumulating. These range shifts are often attributed to species ‘tracking’ their thermal niches as temperatures in their native ranges increase. Our objective was to estimate the degree to which climate change-driven shifts in water temperature may increase the exposure of West Virginia’s native freshwater fishes to mountaintop removal surface coal mining. Mid-century shifts in habitat suitability for nine non-game West Virginia fishes were projected via Maximum Entropy species distribution modeling, using a combination of …

Development Of Novel Models To Study Deep Brain Effects Of Cortical Transcranial Magnetic Stimulation, Farheen Syeda

Theses and Dissertations

Neurological disorders require varying types and degrees of treatments depending on the symptoms and underlying causes of the disease. Patients suffering from medication-refractory symptoms often undergo further treatment in the form of brain stimulation, e.g. electroconvulsive therapy (ECT), transcranial direct current stimulation (tDCS), deep brain stimulation (DBS), or transcranial magnetic stimulation (TMS). These treatments are popular and have been shown to relieve various symptoms for patients with neurological conditions. However, the underlying effects of the stimulation, and subsequently the causes of symptom-relief, are not very well understood. In particular, TMS is a non-invasive brain stimulation therapy which uses time-varying magnetic …

Modulation Of Electron Transport By Metformin In Cardiac Protection: Role Of Complex I, Ahmed Abdul Hussein Mohsin

Theses and Dissertations

Modulation of mitochondrial complex I during reperfusion reduces cardiac injury. Complex I exists in two structural states: active (A) and deactive (D) with transition from A→D during ischemia. Reperfusion reactivates D→A with an increase in ROS production. Metformin preserves the D-Form. Our aim was to study the contribution of maintenance of deactivation of complex I during early reperfusion by metformin to protect against ischemia reperfusion injury. Our results showed that metformin decreased H9c2 cardiomyoblast apoptosis and total cell death following simulated ischemia for six hours followed by reoxygenation for twenty four hours compared to untreated cells. Reactive oxygen species (ROS) …

Fault Classification And Location Identification On Electrical Transmission Network Based On Machine Learning Methods, Vidya Venkatesh

Theses and Dissertations

Power transmission network is the most important link in the country’s energy system as they carry large amounts of power at high voltages from generators to substations. Modern power system is a complex network and requires high-speed, precise, and reliable protective system. Faults in power system are unavoidable and overhead transmission line faults are generally higher compare to other major components. They not only affect the reliability of the system but also cause widespread impact on the end users. Additionally, the complexity of protecting transmission line configurations increases with as the configurations get more complex. Therefore, prediction of faults (type …

Investigation Of Gold(Iii) Complexes With Hiv-Ncp7 And Models, James Beaton

Theses and Dissertations

The medicinal uses of gold date as far back as 2500 B.C. in China. In modern times, gold has been used in the treatment of a number of different human diseases including rheumatoid arthritis, cancer, and viral infections. This dissertation will focus on the development of gold complexes for the purpose of selective inhibition of HIV NCp7, a 55 amino acid zinc finger protein with two Cys₃His zinc binding domains.

NCp7 is involved in a number of viral life cycle processes including activation of reverse transcription, integration, DNA recognition, RNA packaging, and formation of the viral envelope. The …

Effects Of Diabetes On Ovarian Cancer: Data Analysis And Modeling Study, Claire Belay

Theses and Dissertations

Ovarian cancer has one of the highest mortality rates of all gynecological cancers [13]. Further knowledge of risk factors for the growth of ovarian tumors would be beneficial in both the treatment and prevention of this type of cancer. Previous research has shown a positive correlation between diabetes and prostate tumor growth [22], The first aim of this study was to determine the effect of diabetes of ovarian tumor growth. The second aim was to develop a model to predict ovarian tumor growth based on the microenvironment within a patient’s body. The hypothesis was that there would be a positive …

Correlation Of Aqueous Solubility And Anti-Cancer Activity Of Novel Triazolium And Imidazolium Salts, Jared J. Bies

Theses and Dissertations

Novel triazolium, imidazolium, and benzimidazolium salts are known to possess anti-cancer properties and have potential as cancer therapeutics. This thesis describes the aqueous solubility and the differential activity of N,AT-bis-substituted-l,2,4-triazolium bromide salts and N/N'-bis(naphthylmethyl)imidazolium bromide salts against MDA-MB-468 breast cancer cells and PC-3 prostate carcinomas. Using a microscale technique, the partition coefficient (log P) of each salt was determined. The log P value was explored to determine the bioavailability of each derivative.

On Nature's Prototypes: Character Design And Worldbuilding, Taylor J. Bardon

Theses and Dissertations

Nature’s Prototypes is an ongoing narrative series that blends realism and fiction to create both dialectic plot structures and image sets. For substance, the series utilizes biological inquiries into fields, like ecology, pathology, and bioengineering, to imagine action-centric vignettes, where animalistic to monstrous characters become the predominant actors in a post-dystopian worldscape. Throughout these vignettes, these characters are subjected to the trials and tribulations of the catalysts and flux that are imposed upon them. In the series’ larger scope, these vignettes are sequential events within a larger timeline and an original mythos that delve into the implications and the intrigue …

The Impact Of A Manualized Parent Psychoeducational Program And Parents' Knowledge Of Internalizing Behaviors In Youth: A Pilot Study, Emily Morse

Theses and Dissertations

Over 50% of mental disorders have an onset in childhood, and mental health issues during adolescence impact adult psychological, social, and occupational functioning. Parents serve as the primary gatekeepers to child mental health resources, and this research discussed factors that influence parental help seeking, and developed a manualized psychoeducational program for parents was with the aim of addressing barriers. The program was developed based on the need for evidence-supported school-based programs that target parental knowledge, competence, and access to community resources while simultaneously decreasing stigma toward children with mental health difficulties. A quantitative pilot study was conducted as a means …

The Impact Of Experiential Learning In A Service-Learning Context From The Adult Learners' Perspective: A Phenomenological Inquiry, Michele Terrilee Dietz

Theses and Dissertations

In higher education, numerous experiential learning programs are offered to enhance students' learning, including international travel programs, immersion programs, internship programs, and service-learning program. Although students participating in these programs are each higher education institution's number one stakeholders, rarely are they asked about the impact of these programs on their learning, both personally and professionally. For future graduate students, higher education institutions, program designers, and community partners, understanding the perspectives of graduate alumni that have participated in experiential learning programs can be valuable for the future development, assessment, and improvement of such programs. For this reason, the purpose of this …

The Impact Of A Manualized Parent Psychoeducational Program And Parents' Knowledge Of Social Skills In Youth: A Pilot Study, Leanne Mendoza

Theses and Dissertations

Over 50% of mental disorders have an onset in childhood, and mental health issues during adolescence impact adult psychological, social, and occupational functioning. Parents serve and the primary gatekeepers to child mental health resources, and as such factors that influence parental help-seeking were discussed, and a manualized psychoeducational program for parents was developed with the aim of addressing these barriers. The program was developed based on the need for evidence-supported school-based programs that target parental knowledge, competence, and access to community resources while simultaneously decreasing stigma toward children with mental health difficulties. A quantitative pilot study was conducted as a …

Altruism In Psychotherapy: Altruistic Acts As An Adjunct To Psychotherapy, Erin Santos

Theses and Dissertations

This study will explore the impact of altruism on the mind, brain, and body in order to investigate the potential value of using guided altruistic behavior as an adjunct to attaining the goals of psychotherapy. there is considerable evidence from religious and social practices across cultures, groups like Alcoholics Anonymous, and epidemiological research that point to the positive physical and psychological effects of helping others. This idea was initially stimulated by findings that demonstrated that altruism stimulates brain regions also important to the processes and goals of successful psychotherapy. It is hypothesized that engaging in altruistic behaviors will stimulate emotions, …

Mechanical And Material Characterization Of Advanced Materials For Tissue Regeneration, Bailey Kylara Ricketts Jackson

Theses and Dissertations

In natural disasters and military deployment operations, injury to long bone can cause extensive damage and be difficult to treat and heal quickly. Explosive devices and trauma often produce long-bone (>2-cm length) wounds that are more complicated than common fractures. Due to the size and complexity of these injuries, they can be difficult to treat in a battlefield environment and take a long time to heal completely and properly. Even after the long healing period, these injuries often require rehabilitation to strengthen the muscles that have weakened in the wounded limb. For military applications especially, these effects can put …

A Comparison Of The Antimicrobial Efficacy Of Silver Diamine Fluoride And Silver Nitrate: An Ex Vivo Study, Reham M. Alnajjar

Theses and Dissertations

A comparison of the antimicrobial efficacy of silver diamine fluoride and silver nitrate on various cariogenic bacteria: an ex vivo study By: Reham AlNajjar, D.D.S.

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Dentistry at Virginia Commonwealth University. Virginia Commonwealth University, 2019

Thesis Advisor: William Dahlke, D.M.D., Associate Professor and Chair of Pediatric Dentistry, School of Dentistry

Purpose: The use of silver-based antimicrobials is an emerging method for the treatment of dental caries. In this study, the authors compare the efficacy of the two most prominent silver- based therapeutics, silver diamine …

An Examination Of The Scale Of Accurate Personality Prediction’S (Sapp) Test-Retest Reliability Across A Four-Week Period, Taylor Catherine Anderson

Theses and Dissertations

The questions surrounding how well one can truly know him/herself dates as far back as Ancient Greek times, and answering the question has been a difficult task given the complexity of human beings, and a somewhat subjective term such as the “self.” As science and technology continue to advance, so do the attempts to accurately define self-knowledge. Miller (2000) took defining self-knowledge a step further, and developed a test derived from the Sixteen Personality Prediction Questionnaire (16PF) to measure individuals’ level of self-knowledge how well they know themselves, based on their ability to accurately predict their scores on the 16PF. …

Extraversion And Person-Environment Fit: Towards Consideration Of Jungian Theory In Organizational Psychology, Nicholas Rosemarino

Theses and Dissertations

Historically, the field of Industrial/Organizational (I/O) Psychology has relied on the Big 5 taxonomy of personality. The purpose of this study was to examine the value of a Jungian conception of extraversion in the context of the relationship between the physical work environment and person-environment (PE) fit. The MBTI and the NEO-IPIP, representing a Jungian and a taxonomic conception of personality, respectively, were hypothesized to predict the within-person relationship between extraverted environment and PE fit. PE fit was hypothesized to predict perceived satisfaction and perceived performance. To examine these hypotheses, 110 participants were recruited from Mturk and responded to 18 …

Fabrication And Application Of Functional Polymer-Protein Coreshell Nanoparticles, Lin Lu

Theses and Dissertations

The study of polymer-protein nanoparticles is of increasing interest due to their various potential applications in biosensing, imaging, bioseparations, gene and drug delivery, etc. Synthetic polymers can be tailored with different functional groups and properties, therefore, they can serve as platforms to impart proteins with additional features and assist proteins to better perform biological functions. In this dissertation work, we were mainly focusing on the preparation of polymer-protein core-shell nanoparticles by synthesizing new functional polymers and assembling them with proteins for different applications. In chapter 1, a pyridine grafted diblock copolymer P(CL-g-Py)-b-PCL was prepared through ROP and CuAAC reactions. …

Enhancements In Alginate Microencapsulation Technology & Impacts On Cell Therapy Development, Marwa Belhaj

Theses and Dissertations

Biodegradable alginate microcapsules (MC) are recently becoming widely used in the biomedical field, and have shown effectiveness as a drug delivery system. Alginate has been used in microencapsulation due to its simple gelling process, biodegradability, excellent biocompatibility, and its stability under in-vivo conditions. Alginate polymer is a natural polysaccharide derived from brown seaweed and has the ability to polymerize rapidly in the presence of cations to form a porous matrix. In order to advance the previous system for efficient protein and peptide delivery, we further lyophilized the peptide-filled microcapsules and we were able to rehydrate, and test them for sustained …

The Impact Of Communication Deficits On Puberty And Sexual Development In Adolescents On The Autism Spectrum, Ashton Wolfe

Theses and Dissertations

Transitioning into a sexually mature adult is a challenging time for both an adolescent with autism spectrum disorder (ASD) and his or her family. Sexual development begets challenges due to the demands it places on the social and communication skills needed to explore developmental changes that are occurring physically, emotionally and hormonally. A diagnosis of ASD is largely established by quantifying impediments in social and communication skills through observation. With deficits in these skills, one can assume that social and communication difficulties can create obstacles in the sexual development of affected individuals. Our study used quantitative and qualitative measures to …

Matrix Stiffness Modulates Mesenchymal Stem Cell Sensitivity To Geometric Asymmetry Signals, Maria Eugenia Piroli

Theses and Dissertations

Human stem cells hold significant potential for the treatment of various diseases. However, their use as a therapy is hampered by the limited understanding of the mechanisms by which stem cells respond to environmental stimuli. Efforts to understand extracellular biophysical cues have demonstrated the critical roles of geometric and mechanical signals in determining the fate of stem cells. The goal of this study was to explore the interplay between cell polarity and matrix stiffness in stem cell lineage specification. We hypothesize that confining cells to asymmetric extracellular matrix (ECM) islands will impart polarity at a single-cell level and result in …

Plants As Biofactories To Produce Mammalian Tumor Suppressor Micrornas, John Lachlan Macarthur

Theses and Dissertations

MicroRNAs (miRNAs) are small noncoding single stranded RNAs that are considered master regulators of gene expression. They are also an emerging class of therapeutic agents with significant potential for the prevention and treatment of many diseases, including cancer. Many different forms of cancer are associated with loss or reduced accumulation of one or more miRNAs that function as tumor suppressors. In animal models, restoration of missing tumor suppressor miRNAs prevents the initiation, progression and/or spread of the disease. However, the current absence of an efficient method for delivery of therapeutic miRNAs is a critical barrier to their use. The research …

Comparison Of The Performance Of Simple Linear Regression And Quantile Regression With Non-Normal Data: A Simulation Study, Marjorie Howard

Theses and Dissertations

Linear regression is a widely used method for analysis that is well understood across a wide variety of disciplines. In order to use linear regression, a number of assumptions must be met. These assumptions, specifically normality and homoscedasticity of the error distribution can at best be met only approximately with real data. Quantile regression requires fewer assumptions, which offers a potential advantage over linear regression. In this simulation study, we compare the performance of linear (least squares) regression to quantile regression when these assumptions are violated, in order to investigate under what conditions quantile regression becomes the more advantageous method …

Family And School Impact On Psychological Functioning From Childhood To Late Adolescence: A Longitudinal Study Of Rural Chinese Youth, Yang Yue

Theses and Dissertations

The transition from childhood to adolescence can be a significant stressor for youth, leading to increases in internalizing problems for those who are vulnerable. Compared to the mounting research conducted on U.S. youth, children and adolescents in China have received less attention on their psychological adjustment. As Chinese youth constitute roughly 15% of the world’s population (World Health Organization, 2010), and the disproportionate quantity of longitudinal investigation on Chinese youth psychological functioning, understanding the influence of risk and protective factors, and their interactive effects on internalizing problems among Chinese youth is critical.

According to Life Course Theory, Ecological Systems Theory, …

Role Of Mir-489 In Her2 Positive Breast Cancer, Yogin Patel

Theses and Dissertations

Human epidermal growth factor receptor 2 (HER2 or ErBb2) is a receptor tyrosine kinase overexpressed in 20-30% of breast cancers and associated with poor prognosis and outcome. Dysregulation of several micro RNAs (miRs) plays a key role in breast cancer progression and metastasis. In this study, we screened and identified miRs dysregulated in HER2-positive breast cancer cells. Molecular study demonstrated that miR-489 was specifically downregulated by the HER2-downstream signaling, especially the MAPK pathway. Restoration or overexpression of miR-489 in HER2-positive breast cancer cells significantly inhibited cell growth in vitro and decreased the tumorigenicity and tumor growth in xenograft mice. Mechanistically, …

Environmental Links To Ectopic Kidney Diseases, Firas Alhasson

Theses and Dissertations

Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease in the western culture and worldwide. Its morbidity has been raising and it has become a burden on the public health. NAFLD is associated with other metabolic diseases such as Diabetic Mellitus and hypertension. NAFLD is a wide spectrum of symptoms, from an accumulation of fat in the liver (cirrhosis) to production of scar tissue (Fibrosis). The liver along with other distal organs such as the kidney and cardiovascular system can be damaged as ectopic manifestations of NAFLD. Contamination of drinking water with Cyanobacterium toxin (Microcystin) and water disinfection …