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Interactive Effects Of Domoic Acid Allelopathy, Salinity, And Eutrophication On Estuarine Phytoplankton Community Structure, Elise Van Meerssche

Theses and Dissertations

Coastal regions and estuaries are particularly sensitive to the increase in nutrient loading and river runoff, threatening the ecosystems with possible spreads in harmful algal blooms (HABs). As an example, blooms of toxic Pseudo-nitzschia species can release acute concentrations of the neurotoxin domoic acid (DA) in the water column. When ingested at concentrations higher than 20 ppm, DA can cause the death of marine birds, marine mammals and even humans. The main objectives of my research were to determine the drivers of Pseudo-nitzschia abundance and toxicity and to assess how these drivers influenced the phytoplankton community structure and DA allelopathy. …

Two-Electrode Measurements Of Electrostatic Double Layer Potentials With Atomic Force Microscopy, Jason Giamberardino

Theses and Dissertations

In this dissertation, I provide a thorough description of my development of theoretical, analytical, and experimental techniques pertaining to novel measurements of the electrostatic double layer (EDL) using atomic force microscopy (AFM), among other techniques. The EDL refers to the structure formed by ions that exists at the interface between a solid and a liquid. The EDL is an important physical element of many systems and its behavior has been of interest to scientists for many decades. Because many areas of science and engineering are moving to test, build, and understand systems at smaller and smaller scales, this work focuses …

Mechanisms Of Rad51d-Dependent Repair Of Dna And Telomere Damage Induced By Interstrand Crosslinking Agents And Thiopurines, Nicole M. Reilly

Theses and Dissertations

Mutations in homologous recombination (HR) genes increase genomic instability, an enabling characteristic of cancer. However, the status of these same genes can also determine chemotherapy outcomes. RAD51D is a breast and ovarian cancer susceptibility gene that is an important component of HR. Mammalian cells defective for RAD51D have extensive chromosomal aberrations and are more sensitive to the interstrand crosslink-inducing agent mitomycin C (MMC) and the thiopurine 6-thioguanine (6TG). Previously, the RNF138 E3 ubiquitin ligase was identified to promote RAD51D ubiquitination, and loss of RNF138 also increased cellular sensitivity to MMC. Ubiquitination assays were used to show that a 3-ubiquitin modification …

Modulation Of Amyloid-Β Aggregation Via Small Molecules And Glycine Zipper Alterations, Steven Zebulon Vance

Theses and Dissertations

Alzheimer’s disease (AD) is the most common form of neurodegenerative disease. Nationally, AD is the 6th leading cause of death and the only top 10 killer of Americans that cannot be slowed, cured, or prevented. AD is characterized by the deposition of extracellular plaques of aggregated amyloid-β protein (Aβ). Aβ originates from the amyloid precursor protein (APP), a transmembrane protein that is cleaved to form a short and inert protein fragment called Aβ. However, Aβ undergoes a nucleation process wherein aggregates from soluble oligomers to insoluble fibrils are formed. While uncertainty remains as to the exact mechanism, studies have associated …

Evaluation Of Sonocatalytic Degradation Of Ibuprofen And Sulfamethoxazole In The Presence Of Various Catalysts, Yasir Alhamadani

Theses and Dissertations

Over the last two decades, large quantities of products, such as medicines, disinfectants, and personal care products, have been released into surface waters and wastewater treatment facilities by the pharmaceutical and chemical industries which have come to the attention of scientists with regard to their impacts on life in lakes, rivers, and groundwater. Among various types of treatment processes, ultrasonic (US) treatment process was used in this study to investigate the effect catalysts and removal of selected pharmaceutical’s (PhACs) compounds (ibuprofen (IBP) and sulfamethoxazole (SMX)). Carbon nanomaterials (CNMs) have become candidates for numerous applications in nanocomposites, microelectric devices, sensors, energy …

What Makes Killing For Organs Wrong? A Philosophical Defense Of The ‘Dead Donor’ Rule, Adam Omelianchuk

Theses and Dissertations

The purpose of my dissertation is to give a philosophic defense of the so-called “dead donor” rule (DDR) in transplant ethics, something that is sorely lacking in the current literature on the topic. Part of my project is concerned with the rule’s correct formulation: What exactly does it forbid? I answer that it is primarily concerned with prohibiting the killing of the donor for his or her organs, and that it need not be concerned with requiring that the donor be dead before surgery begins (as important as that might be). What is morally important is that surgery not be …

Exploring Alternative Therapeutic Interventions For The Treatment Of Leigh Syndrome, Stephanie Martin

Theses and Dissertations

Leigh syndrome is the most common mitochondrial disease, affecting 1:40,000 live births. It manifests with symptoms including ataxia, cognitive impairment, motor difficulties, and stroke. Leigh syndrome is a genetic condition caused by mutations in either the nuclear DNA or mitochondrial DNA that affects the assembly of the electron transport chains. Death typically occurs around 2-3 years of age, due to respiratory failure, as the body cannot produce sufficient ATP for survival. The genetic basis of Leigh syndrome means that there is no cure, and current treatments of the symptoms have proven ineffective. This thesis proposes two different therapeutic approaches for …

Teaching Musically: Incorporating Dalcroze Pedagogy Into Flute Instruction For The Elementary-Age Student, Emily M. Stumpf

Theses and Dissertations

The current practice of flute pedagogy for the elementary-age student is often focused on skill-building: finger technique, tone development, and note-reading and rhythm skills. Often the teacher will delve into concepts of musicianship only after the student develops a high level of technical skill. I have found it is possible to include musical concepts such as expressive playing, developing an internal sense of rhythm, and improvisation at all stages of the learning process. When the flute teacher functions as the initiator of an aesthetic experience, the development of musicianship becomes just as important as skill-building.

The Dalcroze philosophy of music …

Effect Of Environmental Factors On The Aggregation Behavior Of Silver Nanoparticles In The Aquatic Environment, Kamelia Afshinnia

Theses and Dissertations

Understanding the environmental factors that affect the fate and effects of engineered nanoparticles (ENPs) in the environment is crucial for NP risk assessment. Silver nanoparticles (Ag NPs) readily transform in the environment, which alters their properties and subsequently their transport, fate, and toxicity. The overall aim of this dissertation was to evaluate the effects of Ag NPs physicochemical properties (e.g., size, surface charge, surface coating) and water chemistry (e.g., buffer concentration, organic ligands, natural organic matter) in controlling Ag NPs colloidal stability. This aim was achieved by systematically 1) review and rationalize many studies that investigated the aggregation kinetics of …

Enhance Flow Boiling In Microchannels By Regulating Two-Phase Transport Patterns, Wenming Li

Theses and Dissertations

Flow boiling in microchannels is one of the most promising cooling techniques for microelectronics. Using latent heat by vaporization can significantly improve heat dissipation of high power density electronic devices. Most of the failure of electronic devices is induced by the occurrence of critical heat flux (CHF), which defines the maximum operating conditions. However, the vigorous rapid generation of vapor through phase change leads to chaotic two-phase flows in microchannels, resulting in flowinstability in terms of severe flow, temperature and pressure drop fluctuations. Particularly, the very well-known bubble confinement exacerbates the two-phase flow instabilities and greatly deteriorates heat transfer performance …

Probing Solvent Effects In Aromatic Interactions Using Molecular Balances, Josef M. Maier

Theses and Dissertations

Aromatic interactions play a key role in many important processes, such as the formation and stability of biological and synthetic assemblies, facilitation of molecular recognition processes, and catalysis of chemical reactions. One of the key factors determining the strength of aromatic interaction is the solvent environment. Therefore, developing model systems that can accurately measure the influence or effects of solvents on non-polar interaction strengths is a task of great importance. Over the years, we have been approaching this challenge via designing and analyzing molecular devices that can quantitatively report aromatic interaction energies under various solvent and chemical environments. With this …

Assessing The Strengths And Difficulties Of Typically Developing Siblings Of Persons Born With Lifelong Disabilities, Joseph Joseph Wofford

Theses and Dissertations

In this study, strengths and difficulties of siblings of persons born with disabilities were quantitatively assessed, based on parental perception, in comparison to national norms. Answers of 148 parents to the Strengths and Difficulties Questionnaire (SDQ; Goodman, 1997; Youthinmind, n.d.a.) for children four to seven years old who are siblings of persons born with lifelong disabilities (SPBDs) were gathered and analyzed. The causal-comparative design compared each subscale of the SDQ for SBPDs against their respective norms in the USA for females or males. SPBDs were found to have no statistically significant difference from the norm group on emotional problems, and …

Mechanisms Of Decarboxylation In The Cyp152 Family Of Cytochrome P450s, José Armando Amaya

Theses and Dissertations

Intense interest has focused on the development of enzymes as next-generation catalysts for the production of molecules with biotechnological potential. OleTJE, a member of the cytochrome P450 CYP152 family is an excellent candidate for the generation of advanced biofuels. OleTJE catalyzes the hydrogen peroxide-dependent decarboxylation of Cn fatty acids to produce Cn-1 terminal alkenes. Despite the ability of this enzyme to yield long chain alkenes with nearly exclusive chemoselectivity, its reactivity towards shorter chain length substrates results in undesired levels of hydroxylated side-products, limiting its industrial potential. Previous transient kinetic studies have demonstrated that, despite its unusual chemistry, the reaction …

Effect Of Resveratrol On The Development Of Eczema, Christopher Carlucci

Theses and Dissertations

Atopic dermatitis is a type of eczema characterized by chronic inflammation of the skin, affecting millions of people worldwide. Resveratrol, a naturally occurring stilbenoid, is widely believed to exhibit beneficial effects on a host of chronic diseases. Although some previous studies have aimed to evaluate the effects of resveratrol on the pathogenesis of atopic dermatitis, this relationship remains ill-defined. We have previously established that mast cell activation, remodeling, and cellular infiltration in the hypodermis all begin prior to the IgE-mediated immune response in an atopic dermatitis mouse model, and that this early pathogenesis is directly related to an increase in …

Organic Acids And Amino Acids In Serpentinization Related Fluids From The Atlantis Massif, Nicholas Camper

Theses and Dissertations

Serpentinization is a low temperature, low pressure process that occurs when water reacts with ultramafic rocks. Fluids that have interacted with serpentinites have elevated concentrations of hydrogen and reduced carbon species that can fuel chemolithoautotrophy. International Ocean Discovery Program Expedition 357 drilled a series of boreholes into the Atlantis Massif with the goal of investigating how serpentinization impacts carbon cycling and sustains life in the subseafloor. Bottom water samples (CTD), fluids flushed from the boreholes during drilling (Rock Drill), and fluids that were trapped in the liners of the cores (Liner fluids) were collected. The changes and distributions of organic …

Linking Physiology, Temperature, And Recruitment: A Classic Competitive Story Rewritten By Climate, Maeve Kerrigan Snyder

Theses and Dissertations

Climatic changes have the potential to alter population and community dynamics, ultimately influencing the biogeographic distributions of species. For many organisms, reproduction is physiologically tied to temperature. We tested the hypothesis that a physiological mechanism for reproductive failure in the acorn barnacle, Semibalanus balanoides, would result in predictable patterns of larval recruitment over broad geographic scales. Recruitment density was predicted to be dependent on the duration of permissive temperatures (< 10oC). for successful reproduction in adult populations of S. balanoides. We found that temperature was a reliable predictive variable for recruitment densities throughout our study region. Post-recruitment processes were also considered, specifically the competition between Semibalanus balanoides and Chthamalus montagui, described in Joseph Connell’s 1961 experiment. The competitive hierarchy outlined in this experiment is not consistently observed throughout the range overlap of the two species. Growth and mortality were found to differ dependent on species and latitude, indicating that climate mediates this classic ecological system. Our results provide useful knowledge for refining models of biogeographic shifts. A consistent failure of larval supply and a breakdown of competitive advantage could accelerate the pace of predicted range contraction for S. balanoides. Further investigation of how environmental variables interact with physiology and ecological processes is necessary for accurate predictions of climate change effects

Integrating Self-Determination And Expectancy-Value Theories In Examining The Achievement Of First-Generation College Students: A Latent Profile Analysis Examining Relations Between Perceived Choice, School Valuing, And Perceived Competence And Academic Achievement, Angela Starrett

Theses and Dissertations

First-generation students, who represent more than 40% of entering college freshmen, have lower academic achievement and struggle to persist compared to their continuing-generation peers. Although previous studies have repeatedly shown a deficit model for first-generation students, there is still a lack of clear understanding about the heterogeneity that exists among these college students. While some do struggle to persist, others show marked resilience. Thus, drawing on Self-Determination Theory and Expectancy-Value Theory, this short-term longitudinal study examined whether perceived competence, perceived choice, and positive school value could moderate the risk of being a first-generation college student. A latent profile analysis on …

Understanding The Adsorption And Nucleation Of Transition Metals On A Model Carbon Support, Grant S. Seuser

Theses and Dissertations

Heterogeneous catalysis plays a vital role in the commercial production of chemicals. However, much is still unknown about the fundamental surface chemistry involved in catalyst preparation and the surface chemistry of the catalysts themselves. In the first part of this work, a surface science approach was employed to develop a fundamental understanding of the surface chemistry of strong electrostatic adsorption (SEA) of metal precursors on a model carbon support, highly oriented pyrolytic graphite (HOPG). SEA is a wet impregnation method in which anionic/cationic metal precursors are adsorbed onto positively/negatively charged functional groups by adjusting the pH of the solution above …

The Effects Of A Gamified Curriculum On High-School Students, Joshua Kyle Bastean

Theses and Dissertations

One of the emerging trends in education is gamification, a concept that has been applied in other fields, such as business and advertising, with great success. Gamification can be defined as adding game elements to non-game constructs. This study followed a high-school Algebra II teacher and 10 of her students in an attempt to document the effects of gamification on student academic engagement. Multiple methods of data collection were used to gather data from students and teachers. After analysis, the data showed that gamification had a positive effect on student academic engagement. The results were used to create an action …

Restoration Of Visual Performance And Opsin Expression Within The Retina During Eye Regeneration In The Florida Fighting Conch (Strombus Alatus), Jamie M. Clark

Theses and Dissertations

Conch are slow-moving, herbivorous, marine gastropods that possess prominent camera-type eyes at the ends of long, flexible stalks. Compared to the eyes of other gastropods, those of conch are large (up to 1.5 mm in diameter) and have sophisticated optics that include a lens with a graded refractive index. Conch also have a remarkable ability to regenerate eye tissue: after an eye is lost, a new eye will develop to take its place within weeks. Eye regeneration in conch appears to occur rapidly compared to eye regeneration in other gastropods. Despite our knowledge of the complexity and regenerative abilities of …

The Impact Of A Peer-Teaching Instructional Approach On A Student’S Self-Confidence, John Corral

Theses and Dissertations

This study examines a problem of practice stemming from students not given the same opportunities to use and develop self-confidence and take on higher levels of responsibility in the classroom as they prepare for the 21st century workforce in our society. Recognizing a problem of practice evident in our high school with low-selfesteem in students, this paper studies the effects of implementing a peer-teaching instructional approach to help develop students’ self-confidence and emerging leadership skills (Lockie & Van Lanen, 2008). The central research question addressed in this study is: what is the impact of implementing a peer-teaching instructional approach on …

Changes Of Dispersal Ability In An Isolated Population, Connor Bacon

Theses and Dissertations

Dispersal is a core mechanism in the maintenance of metapopulations. It maintains genetic diversity by connecting subpopulations and generates new populations to replace those that die out. However, as populations become more isolated, as occurs in habitat fragmentation, dispersal becomes more difficult. This should lead to selective pressure against dispersive individuals, causing a reduction in dispersal traits. Over time, this can lead to variation in dispersal traits among populations. We examine this idea using an extreme case of isolation in Euphydryas gillettii, a population that has remained completely isolated for forty years. By comparing this population to a baseline established …

Semiparametric Regression In The Presence Of Measurement Error, Xiang Li

Theses and Dissertations

The error-in-covariates problem has received great attention among researchers who study semiparametric and nonparametric inference for regression models over the past two decades. Without correcting for the measurement error in covariates, estimators for covariate effect usually contain bias. To account for measurement error, much research have been done in mean regression (Liang et al., 1999; Fuller, 2009; Carroll et al., 2006) and quantile regression (He and Liang, 2000; Hardle et al., 2000; Wei and Carroll, 2009). In contrast, there is little research in mode regression and this motivates us to propose semiparametric methods to address this error-incovariates problem in Chapters …

The Effect Of Emphasizing Key Vocabulary On Student Achievement With English Learners, Sarah L. Owen

Theses and Dissertations

This action research seeks to determine the impact of teacher training and the use of specific instructional strategies, per the research-based SIOP® teaching model, on English language arts achievement of formally identified first grade English Learners. The study aims to discover if using strategic techniques to teach vocabulary positively affects language acquisition and comprehension. English Learners have historically lagged behind grade level peers in all academic areas. English Learners tend to have many outside variables that can affect their ability to focus on school. Issues with immigration, class, culture, and race are all relevant topics with this population. This study …

Examining Faith-Based Communities As Leverage Points For The Prevention Of Childhood And Adolescent Obesity, Caroline Glagola Dunn

Theses and Dissertations

Faith-based research and partnerships are becoming more popular as an approach to address the health of underserved populations including racial and ethnic minorities and rural populations. Despite growing interest in faith-health partnerships, little research is available assessing faith-based interventions as potential leverage points for the prevention of childhood and adolescent obesity and the promotion of healthy behaviors. This research aimed to examine the potential for such partnerships within the context of ongoing research partnerships in South Carolina. Specifically, the first study assessed current peer-reviewed literature to determine reporting of reach, effectiveness, adoption, implementation, and maintenance (RE-AIM elements) in faith-based nutrition …

Phylogeny, Ancestral Genome, And Disease Diagnoses Models Constructions Using Biological Data, Bing Feng

Theses and Dissertations

Studies of bioinformatics develop methods and software tools to analyze the biological data and provide insight of the mechanisms of biological process. Machine learning techniques have been widely used by researchers for disease prediction, disease diagnosis, and bio-marker identification. Using machine-learning algorithms to diagnose diseases has a couple of advantages. Besides solely relying on the doctors’ experiences and stereotyped formulas, researchers could use learning algorithms to analyze sophisticated, high-dimensional and multimodal biomedical data, and construct prediction/classification models to make decisions even when some information was incomplete, unknown, or contradictory. In this study, first of all, we built an automated computational …

Role Of Cox2 In Cellular Senescence, Mingxiao Feng

Theses and Dissertations

The survivors of childhood cancers are often associated with adverse late effects including hypoplasia, fatigue, and infertility, which are characteristics of aging. However, the underlying mechanisms of the late effects of chemotherapy are still elusive. We have found that transgenic cyclooxygenase-2 (COX2) expression causes premature aging phenotypes. Since COX2 expression is highly induced by various chemotherapeutic agents, we further investigated the possible link between COX2 and the late effects of chemotherapy. Treatment of juvenile wild-type mice with doxorubicin (DOX), an anthracycline-based chemotherapeutic agent, resulted in premature aging phenotypes and escalated cellular senescence level in various tissues. We found that tissue …

Classifying The Woody Vegetation Community Of Wade Ponds In Colleton County, South Carolina, Wade Biltoft

Theses and Dissertations

Wetland habitats are some of the most biodiverse ecosystems in the world, varying in both physical and biological characteristics. Geographically isolated wetlands are particularly important to the landscape due to their role in the hydrological cycle and as local centers for biodiversity. In parts of the coastal plain of South Carolina, there are geographically isolated wetlands found in concentrated groups with distinctive size, shape, and distribution. Despite their unique appearance and distribution, these wetlands have been scarcely researched or investigated. This project seeks to explore the plant communities that are contained within these ponds in order to understand their composition …

Bodies In Play: Female Athleticism In Nineteenth-Century Literature, Jillian Weber

Theses and Dissertations

My dissertation explores depictions of athletic female characters in nineteenth-century American literature. I argue that there is a rich literary tradition of athletic women, whose origin I date to the 1840s, that has not been explored. To date, scholars have considered athletic women in literature anomalies, a case of a gender-bending female found in a single text, but not part of a longer genealogy of this character type. By examining athletic women over a period of decades and across several genres of American literature we see how this character type has been shaped by authors and culture alike. I illustrate …

On The Scattering Of Topographically Modified Semidiurnal Kelvin Waves Into Internal Waves, Tianyi Zhang

Theses and Dissertations

Semidiurnal tides propagate along continental shelves as a Kelvin wave mode. On wide continental shelves, the shelf topography modifies the Kelvin wave mode into a hybrid Kelvin-edge wave (HKEW), with features of a zero mode edge wave. Its free surface structure and alongshore energy flux concentrate on the shelf, and are sensitive to changes in the shelf width. When a propagating HKEW encounters an alongshore change of shelf width, the adjustment of wave structures results in the scattering of the incident wave into other wave modes, including barotropic and baroclinic Poincaré waves radiating offshore. These dynamics are studied using the …